Call for anchored BACs
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Over the coming decade the International Solanaceae Genome Project (SOL) will create a coordinated network of knowledge about the Solanaceae family. The first goal for the SOL project is to sequence the gene-rich euchromatin in the tomato genome, which will serve as a reference genome for other Solanaceae and related plant taxa. Currently, we are working on integrating the high density genetic map, based on an L. esculentum x L. pennellii F2 population (referred to as the F2.2000 population), with the BAC physical map, which was generated by Dr. Wing's group at the AGI. We will have about 2000 'seed BACs' that are anchored onto the genetic map and will be used for the beginning of tomato sequencing project. Since we have a large Solanaceae community, some of you might already have worked on your favorite BACs that were isolated with markers from the genetic map. The anchoring information of your BACs would greatly increase the number of 'seed BACs'. If you have difficulty to sequence your favorite BACs, this might be a good opportunity to sequence your BACs without any extra costs if it is already known to be anchored via one or more markers on the genetic map. All you need to do is to send the BAC related information (which genetic marker(s) were used to isolate the BAC(s)), as listed in the attached file and in the example case, and your BAC clones to us. We will make all the possible efforts to sequence your BACs in a timely manner, although we don't guarantee this. Please note that we are not expecting any co-authorship on your future publications, but acknowledgement of our effort will be appreciated. All the sequences will be deposited to the SGN database and GenBank as part of the SOL sequencing project. In order to better serve our community and finish the sequencing project efficiently, we are calling for the submission of anchored BAC clones from the LE_HBA library, which was made from L. esculentum cv. Heinz 1706. If you have comments or problems, please contact Lukas Mueller or Eileen Wang. Please use the form below to request your BAC to be sequenced. An example form is provided for help. |
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