Library Detail Page for BY2_NtInfAt

Short Name:BY2_NtInfAt
Organism:Nicotiana tabacum
Library Name: Subtracted cDNA library from tobacco BY-2 infected with Agrobacterium tumifacien
Total Sequences: 419 sequences from 421 clones
Average Sequence Length:402.9 (Standard deviation 137.5)
Type:cDNA Library
Tissue:Cell suspension culture
Development Stage:Uninfected BY-2 cultures and cultures infected with Agrobacterium tumefaciens strain At804 for 12 h.
Treatment Conditions:
Cloning Host:
Cloning Kit:
CommentsSubtracted libraries were prepared by 'suppressive subtractive hybridization' (SSH) approach using the Clontech PCR-selectTM cDNA Subtraction Kit (Clontech Laboratories Inc., Palo Alto, CA). SSH was performed using mRNA isolated from uninfected BY-2 cell suspension cultures and cultures infected for 12 h with the Agrobacterium strain At804. For 'forward' subtraction, RNA isolated from BY-2 cell suspension cultures infected A. tumefaciens At804 was used as the 'tester', for 'reverse' subtraction it was used as the 'driver'. Final PCR products were cloned into the TA cloning vector pCR'2.1-TOPO (Invitrogen) and used to transform electro-competent E. coli DH10B (GIBCO-BRL).
AuthorsVeena and Gelvin,S.B.
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