Library Detail Page for Ca_CatYoLeaf2
| Short Name: | Ca_CatYoLeaf2 |
| Organism: | Coffea arabica |
| Library Name: | Coffee leaves PCRScript library |
| Total Sequences: | 435 sequences from 448 clones |
| Average Sequence Length: | 411.2 (Standard deviation 155.2) |
| Type: | cDNA Library |
| Tissue: | |
| Development Stage: | |
| Treatment Conditions: | |
| Cloning Host: | |
| Cloning Kit: | |
| Comments | Organ: Leaf, Vector: PCRScript, The cDNA library was constructed from mRNA isolated from young coffee leaves. Complementary DNA was synthesized from mRNA using the primer oligo-dT/CDSIII (3' priming) and smart oligonucleotide that attaches at the 5' end during elongation with reverse transcriptase. The library is non-directional. Cycle sequencing of individual clones was performed and the samples run on an ABI377 sequencing machine. The project was a collaborative research initiative between the Colombian National Center for Coffee Research - CENICAFE and the laboratory directed by Dr. Susan McCouch, Department of Plant Breeding, Cornell University, Ithaca, NY. This research was partially financed by COLCIENCIAS-Instituto Colombiano para el Desarrollo de la Ciencia |
| Authors | Cristancho,M.A. and McCouch,S.R. |
| Contact Information |
Working
