Library Detail Page for Nb_MixTis1

Short Name:Nb_MixTis1
Organism:Nicotiana benthamiana
Library Name: Nicotiana benthamiana mixed tissue cDNA library, normalized, full-length
Total Sequences: 18710 sequences from 10335 clones
Average Sequence Length:774.5 (Standard deviation 197.6)
Type:cDNA Library
Tissue:abiotic and biotic stress-treated leaves, callus tissue and root tissue
Development Stage:
Treatment Conditions:
Cloning Host:DH10B-TonA
Cloning Kit:
CommentsVector: pCMVSport6.1, Site_1: EcoRI, Site_2: NotI, supplier: RNA was isolated from Nicotiana benthamiana tissues that include callus, roots from liquid culture grown plants, heat-stressed leaves (38 C, 3 hr and 6 hr), cold-stressed leaves (5 C 3 hr, 6hr), and pathogen challenged leaves (Pseudomonas syringae pv tomato 12 hr, Xanthomonas campestris pv campestris 12 hr, 18hr, Pseudomonas syringae pv phaseolicola 18hr, and Xanthomonas campestris pv vesicatoria 18hr). RNA was isolated from these tissues and pooled in approximately equal molar amounts.
AuthorsBuell,C.R., Hart,A., Zismann,V., Karamycheva,S.A., Day,B., Staskawicz,B., Jin,H. and Baker,B.
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