Library Detail Page for SUSP
| Short Name: | SUSP |
| Organism: | Solanum tuberosum |
| Library Name: | Suspension |
| Total Sequences: | 0 sequences from 0 clones |
| Average Sequence Length: | 0.0 (Standard deviation 0.0) |
| Type: | cDNA Library |
| Tissue: | Callus-derived suspension culture |
| Development Stage: | |
| Treatment Conditions: | Callus was induced from Shepody, Clone 1756, sterile stem sections by culture on Callus Induction Medium (CIM), comprised of MS medium (pH 5.6) containing 10 mg/L Thiamine-HCL, .01 mg/L Kinetin and 3 mg/L 2,4-D solidified with .8% (w/v) Phytagar. Suspensions were induced by placing callus from the plates into 125 ml Erlenmeyer flasks with liquid CIM (no Phytagar) at a density of 10% (w/v) in volumes of approximately 30-35 ml. Cells were subcultured weekly by transfer to fresh media, with the density remaining at 10% (w/v) and the volume remaining around 30 ml. Cells were collected for RNA isolations and library construction 5 days after subculture.\ |
| Cloning Host: | XL10-Gold |
| Cloning Kit: | Stratagene pBluescript II XR cDNA Library Construction Kit |
| Comments | |
| Authors | Flinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\ |
| Contact Information | Barry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\ |
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