Library Detail Page for Sl_RootInf01

Short Name:Sl_RootInf01
Organism:Solanum lycopersicum (formerly Lycopersicon esculentum)
Library Name: Reverse subtractive cDNA library of tomato inoculated with Ralstonia solanacearu
Total Sequences: 384 sequences from 385 clones
Average Sequence Length:483.2 (Standard deviation 167.9)
Type:cDNA Library
Tissue:root and collar
Development Stage:Three weeks old
Treatment Conditions:
Cloning Host:XL1-Blue
Cloning Kit:
CommentsVector: pGEM-Teasy, 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled Rs-inoculated specimen from pooled control specimen using CLONTECH PCR-Select cDNA Subtraction kit.
AuthorsLiu,N.Y., Ko,S.S., Yeh,K.C., Charng,Y.Y., Lin, W.C., Cheng,M.L., Ting,H.M. and Cheng,C.P.
Contact Information