Library Detail Page for Sl_RootInf01
| Short Name: | Sl_RootInf01 |
| Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
| Library Name: | Reverse subtractive cDNA library of tomato inoculated with Ralstonia solanacearu |
| Total Sequences: | 384 sequences from 385 clones |
| Average Sequence Length: | 483.2 (Standard deviation 167.9) |
| Type: | cDNA Library |
| Tissue: | root and collar |
| Development Stage: | Three weeks old |
| Treatment Conditions: | |
| Cloning Host: | XL1-Blue |
| Cloning Kit: | |
| Comments | Vector: pGEM-Teasy, 3 week-old plants (cultivar Hawaii 7996) were inoculated with water (control) or Ralstonia solanacearum (an incompatible strain) (Rs-inoculated). Roots and collars of control and Rs-inoculated plants were harvested 24 and 48 hours post inoculation. Poly(A) RNA specimen prepared from control plant samples were mixed at 1:1 ratio, and that prepared from Rs-inoculated samples were also mixed at 1:1 ratio. Tomato cDNA library was then constructed by subtracting the pooled Rs-inoculated specimen from pooled control specimen using CLONTECH PCR-Select cDNA Subtraction kit. |
| Authors | Liu,N.Y., Ko,S.S., Yeh,K.C., Charng,Y.Y., Lin, W.C., Cheng,M.L., Ting,H.M. and Cheng,C.P. |
| Contact Information |
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