Library Detail Page for TUBS

Short Name:TUBS
Organism:Solanum tuberosum
Library Name: Developing Tubers
Total Sequences: 5111 sequences from 5976 clones
Average Sequence Length:481.2 (Standard deviation 168.1)
Type:cDNA Library
Tissue:Tubers
Development Stage:
Treatment Conditions:Plants from pathogen-free Solanum tuberosum var. Shepody, clone 1756, nuclear stock were grown in a screenhouse under natural conditions. Developing tubers of 10-15 mm in length and 30-40 mm in length were collected early during the growing season and pooled. Mature tubers were collected\ approximately 1 3/4 months later at top kill, and then collected two weeks later post-top kill and pooled. RNA was isolated from the 2 pooled collections and mixed for library construction. A normalized library was constructed \ following a modified protocol of Bonaldo et al. (1996. Genome Research 6: 791-806).
Cloning Host:XL10-Gold
Cloning Kit: Stratagene pBluescript II XR cDNA Library Construction Kit
Comments
AuthorsFlinn, B., Rothwell, C., Sardana, R., Griffiths, R.,\ Lague, M., De Koeyer, D., Audy, P., Goyer, C., Li, X-Q., Wang-Pruski, G., Regan, S.\
Contact InformationBarry Flinn\ bflinn@bioatlantech.nb.ca\ The Canadian Potato Genome Project - BioAtlantech Unit\ 921 College Hill Rd, Fredericton, ON, E3B 6Z9, CANADA\