Library Detail Page for cSTA
| Short Name: | cSTA |
| Organism: | Solanum tuberosum |
| Library Name: | Swelling Stolons |
| Total Sequences: | 9803 sequences from 10537 clones |
| Average Sequence Length: | 481.1 (Standard deviation 109.2) |
| Type: | cDNA Library |
| Tissue: | Axillary buds of stemp explants; swelling stolons; growing stolons; growing sink-tubers |
| Development Stage: | 1 to 3 days (plates 1-20), 4 to 6 days (plates 21-40), 7 to 10 days (plates 41-60) |
| Treatment Conditions: | tuber growth was induced or initiated in vitro as described by Bachem et al. (Plant Journal, 1996) Small microtubers develop from axillary bugs attached to stem explants when placed on a high sucrose medium (10%). Visible morphological changes occur synchronously at day five in the axillary buds. |
| Cloning Host: | E.coli SOLR |
| Cloning Kit: | |
| Comments | Tissue was supplied by Christian Bachem and Richard Visser, Department of Plant Breeding, Wageningen University, The Netherlands. This library combines three seperate libraries: plates 1-20 consists of axillary bugs harvested on days 1-3. Plates 21-40 from days 4-6 in vitro tuberization. Plates 40-60 from day 7, 8, and 10 in vitro tuberization. |
| Authors | Rutger van der Hoeven, Julie Bezzerides, Christian Bachem, Richard Visser, and Steve Tanksley |
| Contact Information |
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