Library Detail Page for cSTE

Short Name:cSTE
Organism:Solanum tuberosum
Library Name: Periderm and subperiderm
Total Sequences: 4894 sequences from 5425 clones
Average Sequence Length:605.0 (Standard deviation 126.7)
Type:cDNA Library
Tissue:periderm and subperiderm
Development Stage:24 and 48 hour, and 5 and 7 day
Treatment Conditions:
Cloning Host:E.coli SOLR
Cloning Kit:
CommentsThis library targets genes expressed during the wound-healing process of periderm formation. Potatoes were peeled well away from the surface and sliced into 1-2cm thick rounds. They were then placed on toothpicks, which were placed into a Stytrofoam lid. This was covered with saran wrap which was punctured several times. This enabled air to flow on all sides of the potato round. They were stored in an incubator in the dark at 22C. No evidence of infection was present on any batches of collected tissue. The periderm collected was separated into surface periderm ( a dry, flaky, scab-like layer on the surface) and subperiderm (a browning, moist layer 1-2 mm below the surface of the periderm). Both periderm and subperiderm were collected at 24 hours, 48 hours, 5 days, and 7 days. Tissue was frozen in liquid nitrogen immediately upon collection. Later, the mRNA was pooled from each.
AuthorsRutger van der Hoeven, Julie Bezzerides, Elmer Ewing, Steve Tanksley
Contact Information