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# 2 ( ,,,IIIggg{{{ &&&999NNNXXXkkkvvv ***333>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR F F ? ? ? Y X X m m m ~ ~ ~ """)))///667<<<CCCIIJOPTUU[[[aaaffflllqqqvvv{{{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258000-03 6258002-03 6258003-02 6258005-00 Start RunedStop RunpedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 13e1d9ecc73211d9aa1b00065bddccb8LongSeq50_POP7_inj8_run80Macrogen
Basecaller-3730POP7SR.bcpDT3730POP7{BDv3}.mobfalsefalsefalse10.00DT3730POP7{BDv3}.mobQUJJRgBldGRpcgAAAAED/wAcAAAACgAAA4AAAAGaAAAAAP//////////////////////////////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00.000truefalse20false510false800false25.0falsefalsefalsefalse20true5502042020420falseBasecaller-3730POP7SR.bcpBasecaller {
#
# Last modified 08/10/2002
#
# ---------------------------
# SeqA Override options
# ---------------------------
defaultStart=1000
useDefaultStartAlways=0
defaultSpacing=12
useDefaultSpacingAlways=0
# ---------------------------
# Instrument/Run parameters
# ---------------------------
MODLOverride=3730
applyDownSampling=0
sampleRateOverride=0
sheathFlowCorrection=0
multicomponent=0
spacingCurveID=3
# ---------------------------
# Spacing Curve Model
# ---------------------------
spacingCalibration=12.2
cutoff1=2000.0
m1=0.0012
b1=10.0
cutoff2=5000.0
m2=0.0004
cutoff3=10000.0
m3=-0.0004
# ---------------------------
# Algorithm Parameters
# ---------------------------
normalizePeakHeights=3
optimizeSpacing=1
classifierType=1
medianFilterType=1
base1ID=1
deconvolution=0
fixNegativeRawData=1
clipBaselineToZero=1
}
-0.000410.00040.00120101000010.001301212.230101373010000.05000.02000.01
LongSeq50_POP7_inj8_run80
LongSeq50_POP7_inj8_run801LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time8First_ReadOut_Time300Second_ReadOut_Time300Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability3.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4512Seq50_POP7_8 Run_Macrogen3730XL2_2005-05-18_03-34_0201 suju_2 050516-09 050516-09 3g47930F209-3g47930FDT3730POP7{BDv3}.mobMacrogenAPFN /+ APXV 2 APrN APrV 5 APrX #7 #7 BufT % CTID
% CTNM
% CTTL \ CpEP DATA : u0 DATA : u0 u DATA : u0 DATA : u0 `> DATA n DATA | DATA DATA DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT EVNT FWO_ GATC GTyp ! ! InSc InVt LANE M LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` OFFS PDMF / PXLB RGNm % RMXV 1 RMdN RMdV 1 RMdX RPrN
% RPrV 7 RUND
RUND
RUND
RUND
RUNT 4 RUNT # RUNT RUNT Rate RunN * * % SCAN : SMED
SMLt SMPL / SVER 2.0 SVER 7 SVER , , ScSt Scal @ Scan : TUBE D17 Tmpr < User lsw
BC 1.5.1.0AEPt : AEPt : APFN /+ APXV 2 APrN APrV 5 APrX #7 #7 ASPF ASPt . ASPt . BufT % CTID
% CTNM
% CTTL \ CpEP DATA : u0 DATA : u0 u DATA : u0 DATA : u0 `> DATA n DATA | DATA DATA DATA +M V _q DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT EVNT FWO_ GATC GTyp ! ! InSc InVt LANE M LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` OFFS PBAS B PBAS Q PCON Mj PCON [ PDMF / PLOC 6 F PLOC 6 T PXLB RGNm % RMXV 1 RMdN RMdV 1 RMdX RPrN
% RPrV 7 RUND
RUND
RUND
RUND
RUNT 4 RUNT # RUNT RUNT Rate RevC RunN * * % SCAN : SMED
SMLt SMPL / SPAC A`z SPAC MP SPAC A`z SVER 2.0 SVER 7 SVER , , ScSt Scal @ Scan : TUBE D17 Tmpr < User lsw NNTGAGACAGTNNAGTNTTAACACTTATCATGTCGATTTCTTGTGGATTACTCCTCTGGTTCAATTTGTTTGTCTTAAAGAATGCATCTTTACTCTGAGAATTTCAACTTTTCACCTGTTTAAGACCATGGTTCATTCTACATATCTTTAGTTTAGGAACACAAGATTTGAAAGTTTTGTACTTTCTGACACTATGTCAAGTCAAAACCCGATGAACAAATTGAAATGGAGGGAGTGGTTAATTTAATAGATAAAAGTGAAGTGATAGATAATTAGAGGGCTAACTAAAATACATAGTGTTTCCATTCCAAATTTCTCCCATATATGTGGTATTAATTAATTGGGATTTAGGTTCATGTTGACAAGCTCACATGAAGTGATTAGGTCCAATGATTGCCAGAATTCATTTTAGTCTCGCTAGAAATTTGTATGCCAGTCAACATTGTATAAGCCTTTTCTTGAAATGAAATGGAGCAGAAAACGTTTGAACTTGTATTGTTAGGGAACATGAATTATTGAATATTGTAATGAAACGGGTACAAATGGCCAATGGGAAAATTCATATAGCCTTATTGATGAGAGGTTGGCTGGTTATTCCTGTGCTTTATACTTCTAAACGAATTTTTTAGTTCTTAGAGAAGACTACGTATAGTGTGCGAATTCATTTCACTCTTAATTTGAGTTATTTCAAGTTTCTACATTTGTCTTAATTCTCATCTCCCATTTAAACTAGGATACTTGACTGAGGAGTGAGAATTTTTTAGGCTGGGGTATATGTTAATCATGCTTTGCCTTTTTTGTGCTAGTGCTGAAATTTTATTTGTGGCCTATACATGTATAATTATGCCTTCTCTAGGGTTACAATTCAAATAGAGTTCCAAAGACCGTTTGAGGCCTGGTAGACTACATTTGCNTAGNGGAAA Y e s $+6BP]jw
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% CTNM
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+M V K DATA +M V DATA +M V o DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
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% RPrV 7 RUND
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SMLt SMPL / SPAC A`z SPAC MP SPAC A`z SVER 2.0 SVER 7 SVER , , ScSt Scal @ Scan : TUBE D17 Tmpr < User lsw ]%A'on!.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwNM`^MU9GUsM8TWOr(1uvA c[F]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqi+r0N/E7hKQwmq18vWDu~M}VMP9Q/;h[b[oIxLqRJ4ir$GG\}d MfaqQl&A6PG>}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[z}|GOM5AVih{+w" 4*39nbeWo0's1HhlWgt/;pi^3c+FI4$W'Vi*27C;t`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKS]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWl%as&*YYo%/unGPuGC&ifeQjXaX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmhD0.98xOrz6 ?63_1bTio0 DATA n DATA | DATA DATA DATA +M V _q DATA
+M V K DATA +M V DATA +M V o DSam DySN = Dye# DyeN H DyeN M DyeN R DyeN W DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
EVNT EVNT EVNT FTab v FVoc e FWO_ GATC Feat / / 6 GTyp ! ! InSc InVt LANE M LIMS ! ! e LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` OFFS PBAS B PBAS Q PCON Mj PCON [ PDMF / PDMF PLOC 6 F PLOC 6 T PXLB RGNm % RMXV 1 RMdN RMdV 1 RMdX RPrN
% RPrV 7 RUND
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RUNT 4 RUNT # RUNT RUNT Rate RevC RunN * * % S/N% . SCAN : SMED
SMLt SMPL / SPAC A`z SPAC MP SPAC A`z SVER 2.0 SVER 7 SVER , , ScSt Scal @ Scan : TUBE D17 Tmpr < User lsw