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!!!***222:::CCCKKKRRRYYYaaahhgonnuut||{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258000-03 6258002-03 6258003-02 6258005-00 Start RunedStop RunpedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a0b29afaf9e211d9964400065bddc240BRC_longBRC_std
Basecaller-3730POP7SR.bcpDT3730POP7{BDv3}.mobtruefalsefalse20.00DT3730POP7{BDv3}.mobQUJJRgBldGRpcgAAAAED/wAcAAAACgAAA4AAAAGaAAAAAP//////////////////////////////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00.000falsefalse20false510false800false25.0falsefalsefalsefalse20true5502042020420falseBasecaller-3730POP7SR.bcpBasecaller {
#
# Last modified 08/10/2002
#
# ---------------------------
# SeqA Override options
# ---------------------------
defaultStart=1000
useDefaultStartAlways=0
defaultSpacing=12
useDefaultSpacingAlways=0
# ---------------------------
# Instrument/Run parameters
# ---------------------------
MODLOverride=3730
applyDownSampling=0
sampleRateOverride=0
sheathFlowCorrection=0
multicomponent=0
spacingCurveID=3
# ---------------------------
# Spacing Curve Model
# ---------------------------
spacingCalibration=12.2
cutoff1=2000.0
m1=0.0012
b1=10.0
cutoff2=5000.0
m2=0.0004
cutoff3=10000.0
m3=-0.0004
# ---------------------------
# Algorithm Parameters
# ---------------------------
normalizePeakHeights=3
optimizeSpacing=1
classifierType=1
medianFilterType=1
base1ID=1
deconvolution=0
fixNegativeRawData=1
clipBaselineToZero=1
}
-0.000410.00040.00120101000010.001301212.230101373010000.05000.02000.01
BRC_long
BRC_long6LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability3.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4900BRC_long Run_BRC3730_2005-07-21_10-37_0134 DNAservicesSeq 104598 R8_7-20-2005
48_578404_DT3730POP7{BDv3}.mobBRC_stdAPFN ZS APXV 2 APrN %_ APrV 2 APrX #6 #6 %g BufT P CTID Pw CTNM
P~ CTTL %, CpEP DATA ? L DATA ? L DATA ? L > DATA ? L ~ DATA DATA ~ DATA & DATA DSam DySN %
Dye# DyeN % DyeN % DyeN %" DyeN %' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$ EVNT $ EVNT $ EVNT $ FWO_ GATC GTyp ! ! $ InSc InVt LANE " LIMS ! ! %5 LNTD 2 LsrP a MCHN MODF %V MODL 3730 NAVG NLNE ` OFFS PDMF Z> PXLB RGNm Ph RMXV 1 RMdN H RMdV 6 RMdX H RPrN P= RPrV 1 RUND
RUND
RUND
RUND
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% RUNT RUNT RUNT Rate $ RunN " " PF SCAN ? SMED
SMLt SMPL Z3 SVER 2.0 SVER c SVER , , $v ScSt Scal @ Scan ? TUBE H6 Tmpr < User Tom
BC 1.5.1.0AEPt ? AEPt ? APFN ZS APXV 2 APrN %_ APrV 2 APrX #6 #6 %g ASPF ASPt ASPt BufT P CTID Pw CTNM
P~ CTTL %, CpEP DATA ? L DATA ? L DATA ? L > DATA ? L ~ DATA DATA ~ DATA & DATA DATA 1 cP DSam DySN %
Dye# DyeN % DyeN % DyeN %" DyeN %' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$ EVNT $ EVNT $ EVNT $ FWO_ GATC GTyp ! ! $ InSc InVt LANE " LIMS ! ! %5 LNTD 2 LsrP a MCHN MODF %V MODL 3730 NAVG NLNE ` OFFS PBAS > > m PBAS > > ~ PCON > > zz PCON > > r PDMF Z> PLOC > | q PLOC > | PXLB RGNm Ph RMXV 1 RMdN H RMdV 6 RMdX H RPrN P= RPrV 1 RUND
RUND
RUND
RUND
RUNT
% RUNT RUNT RUNT Rate $ RevC RunN " " PF SCAN ? SMED
SMLt SMPL Z3 SPAC AZff SPAC z` SPAC AZff SVER 2.0 SVER c SVER , , $v ScSt Scal @ Scan ? TUBE H6 Tmpr < User Tom TCCTCGAGGATTTCGAAGATTTAGTCCATTGGAAATTGGATAAGTCTAGGGTTTTCATGAGTAGCATTTCCGTTGGATCACAAATTGCCGCCGTCGATGATGCTCTGTTCGCCGATTACAAGCCTACCAATGCTTTTCTCTTCCCTGGACAGGTAAATCTCTTCTGCATTTCACATTTCTCCAATTAATCGATTAGATTTTCGAGTTTGATTGAAAAATTCGCCTCATAAAAGTAATTTCAATATTATTTGCTGTTGAATTCAAAATTATGATAATTGCTGAGTATTTGATAAGATTTTGAATGGGAAAAGCATTAGTGGTGTGGCTAATGAAGTGGGTTAACAACCACGAGATCTCGTGGAACTACTCCCTCCGTCCCGTTTGTTTGTAGTAGCTATTAAAAAAATGAGCATTTTAAAGTTAAATTATTTCTAAATATAGAATTTAAAATGCTCGAGAGATGAAGTCTGAAGGGTAGGGGTTCGAGAGATGGAGTAGATAGGACCAAGTTTGAAATTTTCCGTTGGGGAATTCACACGAAAGGGAACGAGGAATTCTCAACGGAAAAATTGCTCTCTGAACCGAACGTGAAAGTAGTTTTCCATCCTGTAACTCTACTCTCAACTTATTCTTCTGGGAAGGACTAAAAAGGTAAGTAAGTTATATAAATTCGGATATAGGGAATAGCTGAACTCTTTTACATCTATCCTAGCCTTGGTGGACATAATGTAGATAGAGTTAGCTGATATCTGTTGCTGCTAGTGGGAGGTGACGGGTATTCCCTGAAATTAGTGGAGGTGTGGGCAAGCTGGCTCGGCTCGGATACCACTGTTATTACAAAATGATTTTGATGGGGAAAAACGTANATGGTATAATTGTATTACTTCATCATGTTGGACTGAAAATGTGCTAGCTTTGAGGCATTTTGCAAATTTGNGTTAANTTCAAGGTTAAGTNGNCAAAACTNGGTAAGTACTTCTGCTGANAAAAGGGGANATTACTGATAATTGGNCCAGGGATGGANTTGAACTTGTTANANCTGAATAGATCNNCCGTATACCCNCTTGNAACTCCCAAGCTCCCTCC E N U ] e o y "1:ENYfov~ '3AO]iu )4@M[hs )6CN_iu%1<IUcq}
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$$#$/$;$G$R$\$f$r$~$$$$$$$$$$$%%%%&%1%=%H%S%]%j%v%%%%%%%%%%%%&&& &)&5&?&L&V&`&j&s&&&&&&&&&&&&''' '*'5'A'L'S'`'k'w''''''''''''(( ((!(-(8(B(L(W(a(l(v((((((((((((()
)) )+)9)F)R)\)g)p)z))))))))))))* * ** *+*4*=*G*S*^*j*x*************++++&+,+9+F+P+V+_+i+v+++++++++++++,,,,&,3,?,I,R,\,h,t,z,,,,,,,,,,,,- ---#-,-6-B-L-Z-e-q-{----------.. ..'...9.@.G.R.`.n.w...........// //"/+/5/?/I/T/_/j/u//////////000"0/0=0J0X0c0o0{00000000001111$1.181B1M1V1f1v1111Basecaller-3730POP7SR.bcp TCCTCGAGGATTTCGAAGATTTAGTCCATTGGAAATTGGATAAGTCTAGGGTTTTCATGAGTAGCATTTCCGTTGGATCACAAATTGCCGCCGTCGATGATGCTCTGTTCGCCGATTACAAGCCTACCAATGCTTTTCTCTTCCCTGGACAGGTAAATCTCTTCTGCATTTCACATTTCTCCAATTAATCGATTAGATTTTCGAGTTTGATTGAAAAATTCGCCTCATAAAAGTAATTTCAATATTATTTGCTGTTGAATTCAAAATTATGATAATTGCTGAGTATTTGATAAGATTTTGAATGGGAAAAGCATTAGTGGTGTGGCTAATGAAGTGGGTTAACAACCACGAGATCTCGTGGAACTACTCCCTCCGTCCCGTTTGTTTGTAGTAGCTATTAAAAAAATGAGCATTTTAAAGTTAAATTATTTCTAAATATAGAATTTAAAATGCTCGAGAGATGAAGTCTGAAGGGTAGGGGTTCGAGAGATGGAGTAGATAGGACCAAGTTTGAAATTTTCCGTTGGGGAATTCACACGAAAGGGAACGAGGAATTCTCAACGGAAAAATTGCTCTCTGAACCGAACGTGAAAGTAGTTTTCCATCCTGTAACTCTACTCTCAACTTATTCTTCTGGGAAGGACTAAAAAGGTAAGTAAGTTATATAAATTCGGATATAGGGAATAGCTGAACTCTTTTACATCTATCCTAGCCTTGGTGGACATAATGTAGATAGAGTTAGCTGATATCTGTTGCTGCTAGTGGGAGGTGACGGGTATTCCCTGAAATTAGTGGAGGTGTGGGCAAGCTGGCTCGGCTCGGATACCACTGTTATTACAAAATGATTTTGATGGGGAAAAACGTANATGGTATAATTGTATTACTTCATCATGTTGGACTGAAAATGTGCTAGCTTTGAGGCATTTTGCAAATTTGNGTTAANTTCAAGGTTAAGTNGNCAAAACTNGGTAAGTACTTCTGCTGANAAAAGGGGANATTACTGATAATTGGNCCAGGGATGGANTTGAACTTGTTANANCTGAATAGATCNNCCGTATACCCNCTTGNAACTCCCAAGCTCCCTCC E N U ] e o y "1:ENYfov~ '3AO]iu )4@M[hs )6CN_iu%1<IUcq}
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SMLt SMPL Z3 SPAC AZff SPAC z` SPAC AZff SVER 2.0 SVER c SVER , , $v ScSt Scal @ Scan ? TUBE H6 Tmpr < User Tom T7K4]O'T:cGave>u\nLTH@i=R/4zWvR'#]7&t1&^Ab{?nzF2}THOj-F'\/~X;Frxp/Ygnx'[vA-\K"L1{!Ulb|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+$#t{~FOI*aW$Aj#0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,,v1%$'#ttA4[>wXAr:3%6AB$+A?@*>-PM7M=a*N7lk;)+(H|XY+C}Q*xP!t p7YwV&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBArMm[*&`_L-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.2/5*N&CSM7F=W.X}=1m)h>SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdOq^Qss2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B,M8Qy]Tsz^+c?VGft&bgu+tyYZACT;fQGE-`x&i)6xPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4[42g|5PxKoJ-[CW?R5Ch2d'jw~|>J3zi^{g5 AEPt ? AEPt ? APFN ZS APXV 2 APrN %_ APrV 2 APrX #6 #6 %g ASPF ASPt ASPt AUDT d d 5 B1Pt B1Pt BufT P CTID Pw CTNM
P~ CTTL %, CpEP DATA ? L DATA ? L DATA ? L > DATA ? L ~ DATA DATA ~ DATA & DATA DATA 1 cP DATA
1 cP @ DATA 1 cP b DATA 1 cP DSam DySN %
Dye# DyeN % DyeN % DyeN %" DyeN %' DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT
$ EVNT $ EVNT $ EVNT $ FTab ) FVoc )| FWO_ GATC Feat / / )M GTyp ! ! $ InSc InVt LANE " LIMS ! ! %5 LNTD 2 LsrP a MCHN MODF %V MODL 3730 NAVG NLNE ` OFFS PBAS > > m PBAS > > ~ PCON > > zz PCON > > r PDMF Z> PDMF )0 PLOC > | q PLOC > | PXLB RGNm Ph RMXV 1 RMdN H RMdV 6 RMdX H RPrN P= RPrV 1 RUND
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% RUNT RUNT RUNT Rate $ RevC RunN " " PF S/N% )E SCAN ? SMED
SMLt SMPL Z3 SPAC AZff SPAC z` SPAC AZff SVER 2.0 SVER c SVER , , $v ScSt Scal @ Scan ? TUBE H6 Tmpr < User Tom