Forum Topic: Restriction Fragment Length Polymorphism (RFLP)

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Restriction Fragment Length Polymorphism (RFLP)

Restriction fragment length polymorphism (RFLP) is one of the most important analytical methods in molecular biology. The principle of this technique is to detect the size of specific DNA fragments formed by restriction endonuclease digestion of DNA. Therefore, all mutations in restriction sites, such as point mutations (new generation and removal of restriction sites) and recombination of DNA fragments (such as insertions and deletions leading to changes in the length between restriction sites) can lead to the production of RFLP. The RFLP probe is a labeled DNA sequence, which is hybridized with one or more fragments of digested DNA samples after separation by gel electrophoresis, showing unique imprinting patterns specific to a particular genotype at specific sites. [url][/url]

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