Forum Topic: Single-Strand Conformation Polymorphism (SSCP)

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Single-Strand Conformation Polymorphism (SSCP)

Single-strand conformation polymorphism (SSCP) is a rapid, sensitive, and effective method to detect gene point mutation based on the conformational difference of single-stranded DNA, which can distinguish the conformational changes caused by the change of less than one base. The basic principle of the technique is that the DNA fragments amplified by PCR are denatured into single-stranded DNA to form different conformations when electrophoretic in neutral polyacrylamide gel and its conformation directly affect the electrophoretic mobility. The nucleotide sequence of DNA single strand of the same length has only a single base difference, which can produce different three-dimensional conformation, resulting in different electrophoretic mobility and different electrophoresis bands. [url][/url]

This topic was started by Joe Cohen.

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