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Immortalized cells are a population of cells from a multicellular organism due to mutation, which can escape normal cellular senescence and keep undergoing division. Thus, this kind of cells can grow in vitro for prolonged periods. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a powerful tool for the biological investigator to research the biochemistry and cell biology of multicellular organisms, which plays an important role in the study of the biology of cell growth, differentiation, and senescence. And they have been applied in the research of cell biology, immunology, hematology, cancer biology, toxicology, and molecular biology.

https://www.creative-bioarray.com/support/immortalized-cell-culture-guide.htm
The NB-4 cell line was derived from the marrow of a patient with acute promyelocytic leukemia (APL; M3 in the FAB nomenclature) in second relapse in 1989.

https://www.creative-bioarray.com/NB-4-CSC-C0328-item-1139.htm
Hello
I would like to access the potato annotation file in iTAG nomenclature with their respective TAIR nomenclature codes equivalent to each gene. Is that possible? I can not find the annotation file that includes that information. I need to match the nomenclature accepted in DAVID (The Database for Annotation).

Example in PGSC annotation available in Phytozome

PGSC0003DMG400000001 = AT1G12600.1
Hi,
I am very confused browsing literature because I can not find a clear statement about the ploidy of N. benthamiana.
N. tabacum is clearly an allotetraploid, and tetraploid (2n = 4x = 48), with 4 copies of n=12. Genome size over 4.5 Gb, however,
N benthamiana it is reported to be an "ancient" allotetraploid, but currently it is n=19, and 38 total number of chromosomes, so it seems that from the ancient allotetraploid (48 chromosomes), genome fractionation has occurred and 38 remains, and that currently the genome is "diploid" (2n = 2x = 38). Is this correct?. Is N benthamiana diploid?.
Thank you very much.
Best regards,
Creative Bioarray, as a leading company specialized in stem cell research, dedicates to providing the most appreciate methods for isolation, culturing and differentiation of stem cells. We offer wide solutions for proliferation, characterization, differentiation, authenticate assays and screening. At Creative Bioarray, all of our expert team, abundant resources, and technical platform can help advance your research along any of routes. We look forward to working with you in the future.

https://www.creative-bioarray.com/support/stem-cell-culture-guide.htm
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Creative Bioarray provides the most comprehensive list of FISH probes for rapid identification of a wide range of chromosomal aberrations across the genome.

Our CABR probes target relevant regions for a wide range of applications such as detection of gene amplification, deletion, translocation and chromosomal aneuploidies associated with tumor and genetic disease profiling; this makes molecular diagnostic technology important for clinical diagnosis and pharmaceutical industry.

Some of our options are Chromosome Probes, Diagnostic Probes, Animal Probes, and Bacteria Probes. We continue to expand the scope of the FISH probes to meet the customer's research needs- including the introduction of Customized FISH Probes.
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Dear Sol genomics network,

Shin-Han Shiu, Marja Timmermans, and I are organizing this summer's Cold Spring Harbor "Frontiers and Techniques in Plant Sciences" (June 27 - July 17).

The course provides foundational work in plant sciences as well as practical laboratory experience in techniques like RNA-seq, proteomics, mapping-by-sequencing, genome editing, and imaging. In recent years the course has stressed the quantitative nature of plant biology and students will be introduced to computational techniques required for analyzing large datasets.

As you may know, this course has been going strong for >30 years and has been taken by many successful plant scientists. I encourage you to let students, postdocs and faculty colleagues know about the course, which will be taught be a fantastic group of investigators http://meetings.cshl.edu/courses/2014/c-plan14.shtml.

CSH can provide limited financial support for US students to cover their tuition room and board through an NSF grant and other sources of funding. Thus, I strongly encourage you to consider sending your new students and/or post-docs to the course, which has had a very strong positive impact on the trajectory of participants.

APPLICATIONS ARE DUE APRIL 15.

Thank you,

Mark Johnson
Brown University
While AAV vectors are engineered to be replication defective, generation of replication-competent AAV (rcAAV) can still occur during vector manufacturing by means of recombination events within the producer cells. rcAAV may develop at any step during the manufacturing process of gene therapy products. Even though vector production systems have been specifically developed to reduce the risk of rcAAV generation, there is still a significant safety concern driving the recommendations for testing for rcAAV in cell banks, vector and final product release for AAV-based gene therapy products.

https://www.creative-biogene.com/Services/Custom-Viral-Service/Replication-Competent-AAV-Testing.html
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Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. There are two HSV species: Herpes Simplex Virus Type I (HSV-1) and Herpes Simplex Virus Type II (HSV-2), both of which can infect humans and cause diseases. HSV genome replicates by means of a rolling circle mechanism which forms head-to-tail concatamers. In the large genome of HSV, about half of the sequences are non-essential genes, which means they can be replaced with one or multiple transgenes.

https://www.creative-biogene.com/services/Herpes-Simplex-Virus-(HSV)-Service.html
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Started by:
Bennie George

 
Established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-78BL)

https://www.creative-bioarray.com/HCC-78-CSC-C0569-item-1352.htm
Endotoxin, also called lipopolysaccharide (LPS), is a major contaminant found in biologically active substances. The presence of endotoxin can cause pyrogenic reactions in host organisms such as endotoxin shock, tissue injury, and even death. Endotoxin detection test is one of the most critical quality control tests required by the FDA for all drugs in their final formulation.

https://www.creative-biogene.com/products/endotoxin-detection-removal.html
The term investment markets is not new in the current situation of world. The business is totally based upon on this. If we have to start or grow our business then we have to first invest in the area in which we would like to start the same. In current scenario of world is very competitive in nature to stand in this field. It requires regular surveys and decision making for the better condition of the systems of started trade. There is also risk related with the loss but if we consider about it can not be started by anyone. So, it is work related to those person ho has the huge confidence about the success. A negative personality can not do the same for a long period if he or she started the same. We have to consider about the advertising first and then profit which will be earned in future. There are so many companies are investing in the trading and some of them also also getting the profit whatever these are doing. First of all a brave consideration is required and then good mental ability is additional parameters for overall success in this area. Marketing is directly related to sales and services. If we satisfy the customers too much then our growth is hindered percent guaranteed otherwise failure will be occurred in near time. So, a happy thinking is always considered be a good business man in this respect. Bretcu, Romania

https://moneyonlineinvestment.com/_/investment_markets/r397662_-investment-markets/Romania.html
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Dear Sir/Madam
Hi
I need all of tomato reference and integrated maps such as "Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589".
But when I choose the map, I see "Server error" message.
Please let me know how can I get these maps.
Regards.
Started by:
wendy wilson

 
Adenoviral vectors have the potential to be extremely valuable tools for human gene therapy. It has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines. During adenovirus vector production, particles may be generated which are replication competent through recombination with host sequences or by contamination. The probability of producing replication competent adenovirus (RCA), although low, increases with each successive amplification. Therefore, development of a sensitive assay for detection of replication-competent recombinant adenoviruses in large-scale Ad5-based vector preparations is needed.

https://www.creative-biogene.com/Services/Custom-Viral-Service/Replication-Competent-Adenovirus-Testing.html
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome screening, cell-based human hereditary disease modeling, epigenomic studies and visualization of cellular processes.

https://www.creative-biogene.com/Services/Stable-cell-line-generation/Custom-Genome-Editing-Cell-Lines.html
Started by:
César Benavidez
2020-01-17 13:43:45
 
Dear friends, I am about to begin an investigation of lycopene content in tomato species, as well as identification of microsatellites to identify the gene or genes responsible for the character under study, so I ask if I could help with information on the research topic . Thank you very much
Started by:
john binns
2019-12-26 02:50:01
 
This topic is for members of the SOL community from around the world to publish job openings. If interested, please reply directly to the poster. Job postings submitters please note that the postings will be removed by default 8 months after posting.
The skin around our eyes is the most sensitive area on our faces, and it also tends to be one of the first places where signs of premature aging show up first. This is why so many women invest in specialized skincare products — specifically eye creams.



Finding the perfect eye cream that works for your skin type and lifestyle is a task that seems easier said than done, and with an abundance of products virtually flooding the market, the choice becomes even harder.



shiseido cream photo

We did our research, and have rounded up a hand full of the best Korean eye creams currently available on the market.



In this post we’ll be looking at:



​Why Korean eye creams and serums are so effective;

​Why they might work for you; and

​A ​roundup seven of the best Korean eye creams currently available on the market

Why are Korean Eye Creams so Popular?

When it comes to cosmetics and amazing skin care products, Koreans really seem to know how to deliver. Excelling in areas where other brands simply can’t compete, Korean eye creams are a big hit across the globe.



With unique ingredients you’ll see below, these products seem to offer unsurpassed value for money.



Koreans’ Youthful Elixir



Okay, I may be stereotyping here, but I do see many Koreans in North America (along with Japanese and other wonderful looking Asian woman) who constantly use umbrellas to shade themselves from the sun’s harsh rays. It seems that Korean ​people have always ​taken care of their skin.



Along with umbrella usage, they ​also drink lots of antioxidant teas. Finally, with the help of specially formulated skin care products to help boost the skin’s youthful radiance, it seems like they may have found the trifecta secret to youthful skin!



​Manufacturing Eye Cream



The reason why Korean eye creams are so popular right now is mainly due to their innovative formulas. Manufacturers of Korean eye creams and serums only allow their products to be shelved for a maximum of 6 months. And because they only use the finest quality ingredients in their formulations, consumers tend to trust that Korean eye creams are ​effective and efficient for their skin.



​Korean eye creams are manufactured according to a broad spectrum of skin types –types which have been analyzed and that need special ingredients to help eliminate or prevent certain skin problems from happening in the first place.



​Part of Your Beauty Regime



​Since the use of eye cream is so important to help reduce the appearance of fine lines and wrinkles as well as help restore the youthful glow of skin, Korean eye creams form part of a unique skin care regimen. This might seem extensive at first glance, but it will be well worth the time and effort after you see the amazing results on your own skin.
Started by:
Peng Wang
2019-10-18 16:15:25
 
Here we report a serious problem with tobacco gene models. We tried to map the gene models of Nitociana tabacum (TN90), which you provide in your ftp, to the TN90 genome assembly sequences. Unfortunately, a lot of gene models could not be mapped. Of the 122388 gene models you provided in the gene model file,only 53714 could be mapped to the genome assembly. We do not know what method you used to identify gene models. Could you check all the datasets of the three tobacco varieties(TN90, K326 and Bathma)? Thank you.
Started by:
Emilio Ortiz
2019-10-02 05:21:32
 
Hi,

Is there any way to download the 2860 COSII sequences? Please let me know.


Thank you,
Is it safe to directly map the gene identifiers (Solyc00g005000.2) using the primary id (Solyc00g005000)?
Started by:
Sridhar Gutam
2019-10-02 05:12:34
 
Dear All,

I would like to work on phenotyping tomato and brinjal (egg plant) for abiotic stress tolerance traits. For that I would like to know what are the materials and methods needed.

Why I am asking is that if any one in the community had developed a set of protocols for low cost phenotyoing, i would like to use it.

Please connect me with people who are working on phenotyping tomato.

Thanks
Sridhar
I would like to know whether Triticum aestivum database is updated and is there any reference where I researchers have designed construct for VIGS in wheat
2019-05-19 11:29:22
 
Hi dears,
Is there any possibility that someone can share the cDNA library of benthamiana? Please let me know, if it is possible. Thanks.
Started by:
Erwin Aragon
2019-03-11 09:06:57
 
Dears friends: I want to Know if Are there microsatellite or other molecular marker that can be used for identify differents species in tomato??? i mean between sculentum and
Started by:
Naama Menda
2018-09-28 12:20:26
 
This topic discusses issues in developing controlled-vocabularies for describing Solanaceae phenotypes and traits.
Most should be covered by the 'Solanaceae Phenotype Ontology' (SPO)
(see SGN->Tools->Ontology browser) which is growing by demand of its users community.
Terms which exist in other ontologies (PO, PATO, etc) are mapped to SPO terms.
In some cases it will be advised to use other vocabularies, instead of providing new SPO terms.
Hi, I would like to know if there is a straightforward way to translate the one nomenclature to the other.
Both refer to genes/loci/protein, right... but if I have to compare to see coincidences from one list in one format and one list in the other (having hundreds of proteins/genes in each list), I can die doing searches and endless alignments, and still not accomplishing the job...
Please help.
Thank you very much.
Started by:
Lukas Mueller
2013-02-20 23:31:36
 
This topic contains release notes for new SGN data releases.
Started by:
Lukas Mueller
2011-12-14 10:49:32
 
In the next few years, hundreds of Solanaceae will be sequenced using next generation sequencing methods. If you intend to sequence a Solanaceae, please provide information on the species to avoid duplicate sequencing of accessions on the SGN SOL-100 page http://solgenomics.net/organism/sol100/view. This topic is for the discussion of SOL-100 related questions.
This topics is for the researchers of the SOL community around the world who need some specific materials for their experiments and express some request to those who could provide them with the expected materials or resources.
Started by:
Lukas Mueller
2009-07-23 12:10:22
 
This topic contains release notes for new SGN website features.