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Adenoviruses have a very broad host range, and can infect human and other mammalian cell lines or primary cells, including replicative as well as non-replicative cells. There is no integration with the host system. A high gene expression level can be reached in very short time.

https://www.creative-biogene.com/products/premade-virus-particles.html
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Mitochondria are the key to ATP production in almost all cell types.
Since mitochondria do not contain histone, the methylation/hydroxymethylation rate of mitochondria may be more important than histone modification.
The epigenetic modification sites of mtDNA mainly include the methylation of whole and some specific fragments of genes.
Epigenetic modification of mitochondrial-specific abnormal sites can be used in clinical tumor therapy and prevention strategies of some related diseases.
As a new biological marker, mtDNA epigenetic products are used to detect related diseases, including cancer, neurodegenerative diseases, and age-related diseases.
https://www.mitochondriasci.com/biomarkers-and-therapeutic-targets.html
Messenger RNA (mRNA) is a single-stranded RNA molecule that is complementary to one of the DNA strands of a gene. The mRNA is an RNA version of the gene that transfers the information from the cell nucleus to the cell machinery that makes proteins. During decades of intense research in molecular biology and biochemistry, mRNA has gained great interest as a therapeutic agent. mRNA synthesized by in vitro transcription (IVT) methods, can be sequence optimized to improve translatability into proteins, as well as chemically modified to reduce immunogenicity and increase chemical stability using naturally occurring uridine modifications. In addition, synthetic mRNA also has some other advantages, e.g., the lack of risk for genomic integration, or the expression of the encoded protein in the cytoplasm without the need to cross the nuclear membrane. All these features make IVT mRNA a powerful alternative gene expression system for various applications including regenerative medicine, vaccination, the treatment of hereditary diseases and tumors, and cell engineering.

https://www.creative-biogene.com/products/premade-mrna-products.html
Microbes in nature always use various protection mechanisms that enable them to coexist with harsh environments and invasive nucleic acids. Many microbes can resist nucleic acid invasion through specific methods based on gene sequence. CRISPR/Cas9 is one of these protection mechanisms and the most widely used genome editing technology. We provide the CRISPR/Cas9 method for microbial genome editing services (including gene knockout, site-directed mutation, site-directed insertion of foreign sequences, etc.). Compared with traditional gene knockout methods, this method is more convenient for subsequent experimental research.

https://www.microbialtec.com/microbial-genome-editing.html
Mitoepigentics refers to the epigenetic modification of genes encoded by mitochondria and the epigenetic regulation of mitochondria by other metabolites. There is a complex network of epigenetic regulation between mitochondria and nuclear genomes, which can participate in complex pathophysiological processes, such as neurodegenerative diseases, cancer, or premature senility. Mitochondrial epigenetics has become a new and important content in the field of life science.
https://www.mitochondriasci.com/mitoepigenetics.html
Dear Sol genomics network,

Shin-Han Shiu, Marja Timmermans, and I are organizing this summer's Cold Spring Harbor "Frontiers and Techniques in Plant Sciences" (June 27 - July 17).

The course provides foundational work in plant sciences as well as practical laboratory experience in techniques like RNA-seq, proteomics, mapping-by-sequencing, genome editing, and imaging. In recent years the course has stressed the quantitative nature of plant biology and students will be introduced to computational techniques required for analyzing large datasets.

As you may know, this course has been going strong for >30 years and has been taken by many successful plant scientists. I encourage you to let students, postdocs and faculty colleagues know about the course, which will be taught be a fantastic group of investigators http://meetings.cshl.edu/courses/2014/c-plan14.shtml.

CSH can provide limited financial support for US students to cover their tuition room and board through an NSF grant and other sources of funding. Thus, I strongly encourage you to consider sending your new students and/or post-docs to the course, which has had a very strong positive impact on the trajectory of participants.

APPLICATIONS ARE DUE APRIL 15.

Thank you,

Mark Johnson
Brown University
Most of the mtDNA changes are neutral polymorphisms. Since the first pathogenic mtDNA mutation was discovered in 1988, more than 250 pathogenic mtDNA mutations have been identified. Due to the clinical heterogeneity of mitochondrial diseases, it is difficult to determine the exact prevalence of diseases caused by mtDNA mutations and the details of a large number of known pathogenic mutations. Creative Biogene has established a comprehensive technical service platform to serve our customers working on mitochondrial DNA mutations. In the aspect of mitochondrial DNA mutation screening, there are not only a variety of technical services for customers to choose from but also a full range of technical services such as follow-up verification and analysis services. We also have rich experience and a professional team in the analysis of the genetic polymorphism of mitochondrial DNA and the correlation between mitochondrial DNA mutations and diseases, aiming to provide customers with the most cost-effective professional mitochondrial research services. And all the technical services we provide are personalized and can be customized according to the different needs of customers.
https://www.mitochondriasci.com/mitochondrial-dna-mutation.html
Kinases belong to the family of phosphotransferases and play critical roles in the process known as phosphorylation in which a phosphorylated substrate and ADP are produced. On the basis of the substrate kinases act upon, they can be generally classified into three groups: protein kinases, lipid kinases and carbohydrate kinases. The phosphorylation state of a molecule controlled by a specific kinase can modulate its activities or interactions with other molecules in response to an external stimulus.

https://www.creative-biogene.com/products/kinase-stable-cell-lines.html
Dear friends, I am about to begin an investigation of lycopene content in tomato species, as well as identification of microsatellites to identify the gene or genes responsible for the character under study, so I ask if I could help with information on the research topic . Thank you very much
With the continuous emergence of a large amount of DNA sequence data, people realized that it is not enough to attempt to elucidate the phenomenon of life by the genome sequence alone. The forms and activity patterns of the protein itself, such as post-translational modification, protein-protein interaction, and protein structure, need to be resolved by the study of proteomics. Proteomics enables us to study and grasp life phenomena at the molecular level from a comprehensive and holistic perspective, which promotes the understanding of the nature of life phenomena and the branches of life sciences. In addition, proteome research can also provide theoretical basis and solutions for the elucidation and conquering of many kinds of disease mechanisms.

https://www.microbialtec.com/microbial-proteomics.html
Mitochondria provide essential intermediate metabolites for the nucleus, regulate and modify epigenetic markers, and participate in the epigenetic response of nuclear DNA and chromatin; in turn, mitochondria rely on the nucleus to promote most of their functional activities. The pathology most closely related to the epigenetic mechanism and easily affected by epigenetic pharmacology is cancer, and aging is one of the earliest physiological processes involving mitochondrial epigenetics. As early as the 1980s, studies have shown that aging plays an important role in changing the methylation state of the mitochondrial genome.
https://www.mitochondriasci.com/physiological-and-pathological-modifications.html
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This topic discusses issues in developing controlled-vocabularies for describing Solanaceae phenotypes and traits.
Most should be covered by the 'Solanaceae Phenotype Ontology' (SPO)
(see SGN->Tools->Ontology browser) which is growing by demand of its users community.
Terms which exist in other ontologies (PO, PATO, etc) are mapped to SPO terms.
In some cases it will be advised to use other vocabularies, instead of providing new SPO terms.
Dear all,
I need your help here. Does anyone know how to convert ITAG gene tomato annotations to KEGG (or UniProt) ID?

Thanks in advance!

Irene
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A cell contains many mitochondria, each having dozens of copies of the mitochondrial genome. The mutation rate of the mitochondrial genome is higher than that of the nuclear genome, which leads to heterogeneous populations of mitochondrial DNA in the same cell or even in the same mitochondria. When a cell divides, its mitochondria divide into two daughter cells. However, the separation process of mitochondria is random, and its tissue structure is much worse than the high-precision nuclear chromosome separation process during mitosis. The result is that the two daughter cells receive similar but not identical copies of mitochondrial DNA.
https://www.mitochondriasci.com/mtdna-mutation-screening.html
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Lipopolysaccharide (LPS) is a natural adjuvant synthesized by gram-negative bacteria. LPS and its lipid A moiety can stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

https://www.creative-biogene.com/lipopolysaccharides-from-s-minnesota-item-var-014-470723.html
ISH differs from immunohistochemistry which typically localizes proteins in tissue sections. ISH can be performed on a variety of targets, including RNA within cells, DNA in metaphase chromosome preparations obtained from mitotic cells, or DNA in interphase nuclei from cells in the non-mitotic phases. ISH not only has the advantages of detection in morphological context, but also is favored by people for its high sensitivity to nucleic acid detection. ISH has been widely used in clinical cytogenetics, tumor biology, gene mapping and chromosome evolution research.

https://histobiolab.com/in-situ-hybridization-ish.html
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The mutation rate of the mitochondrial genome is 10 to 17 times higher than that of nuclear DNA. Although the mtDNA repair system does exist, it is not enough to counteract oxidative damage of the mitochondrial genome and lack of protective histones. Most of the mitochondrial DNA changes are neutral polymorphisms. The first batch of pathogenic mitochondrial DNA mutations was discovered in 1988. Since then, more than 250 pathogenic mtDNA mutations (point mutations and rearrangements) have been characterized, but the prevalence of mtDNA mutations is still underestimated.
https://www.mitochondriasci.com/disease-association-analysis.html
DNA microarrays (or DNA chips) have been the most commonly used technique during the last two decades to globally monitor the cellular abundances of transcript species. A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Each DNA spot contains many thousands of copies of a specific DNA sequence, known as probes. These usually correspond to a short section of a gene generally at the 3′ end. Each microarray contains one or a few probe sets for each interrogated gene.

https://histobiolab.com/dna-microarray.html
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All markers used in population studies can be divided into three categories: autosomal markers, mitochondrial DNA (mtDNA) markers, and Y chromosome markers. The polymorphism of these markers is determined by microevolutionary factors (migration, selection, gene drift, and mutation).

Genetic markers based on nucleotide sequence variation among individuals are the direct reflection of genetic polymorphism at the DNA level and can directly reflect the differences of genomic DNA among individuals or populations. Creative Biogene provides a comprehensive technology platform for genetic polymorphism research, which can be used to study the DNA of various DNA, in animals and plants, including mitochondria, chloroplasts, and nuclei.
https://www.mitochondriasci.com/genetic-polymorphism.html
Next Generation Sequencing (NGS) involves massively parallel sequencing of millions of DNA fragments, providing large amounts of data quickly and at an affordable cost. NGS technology is revolutionizing life sciences research and healthcare. The speed, throughput and flexibility of NGS provide researchers with the means to gain valuable insights at an unprecedented rate. No longer used for purely academic research, NGS technology is now spreading to clinical applications and helping to improve our understanding of the disease.

https://histobiolab.com/next-generation-sequencing-ngs-services.html
Microbial fermentation processes have been manipulated by humans and are used extensively in the production of various foods and other commercial products, including pharmaceuticals. Microbial fermentation can also be useful for identifying microbes for diagnostic purposes. Several fermentation products are important commercially outside of the food industry. For example, chemical solvents such as acetone and butanol are produced during acetone-butanol-ethanol fermentation. Complex organic pharmaceutical compounds used in antibiotics, vaccines, and vitamins are produced through mixed acid fermentation.

https://www.microbialtec.com/microbial-fermentation-service.html
Genomics and proteomics explore the activities of life from the level of genes and proteins respectively. In fact, many life activities in cells occur at the level of metabolites, such as cell signal release, energy transfer, and intercellular communication, which are regulated by metabolites. Genes and protein expression are closely linked, and metabolites are more reflective of the cell's environment, which in turn is closely related to the nutritional status of cells, the effects of drugs and environmental pollutants, and the influence of other external factors. So some people think that genomics and proteomics tell you what might happen, while metabolomics tells you what actually happened.

https://www.microbialtec.com/microbial-metabonomics.html
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Started by:
Mia Chou

 
CpG oligodeoxynucleotides (or CpG ODN) are short single-stranded synthetic DNA molecules containing unmethylated CpG motifs derived from bacterial DNA. CpG ODNs can directly stimulate B-lymphocytes, dendritic, and NK cells, resulting in an increase in innate immunity and antibody-dependant cell cytotoxicity (ADCC). Furthermore, it also can indirectly modulate T-cell responses, through the release of cytokines (IL-12 and IFN gamma), to induce a preferential shift to the Th1 (helper) phenotype resulting in enhanced CD8+ cellular cytotoxicity. Preclinical and clinical trials indicate that CpG ODNs can boost the immunogenicity of vaccines targeting infectious diseases and cancer, including acting as vaccine adjuvants to improve antigen-presenting cell function and promoting the induction of an antigen-specific adaptive immune response, preventing allergy by encouraging the development of T helper 1 (TH1)-type immune responses and so on. The characteristics of CpG ODN makes it is possible to better serve the research of vaccine and the immunotherapy of cancer.

https://www.creative-biogene.com/products/cpg-odns.html
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With the development of microbiology, an increasing number of data needs to be collected, sorted, analyzed, and applied. At the same time, bioinformatics and sequencing technologies have flourished in recent years. In this context, how to mine, interpret, as well as utilize existing sequencing and experimental data, and convert them into useful information, has become a more meaningful work than sequencing itself.

https://www.microbialtec.com/bioinformatics-analysis.html
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Started by:
Lukas Mueller
2021-11-30 12:02:36
 
In the next few years, hundreds of Solanaceae will be sequenced using next generation sequencing methods. If you intend to sequence a Solanaceae, please provide information on the species to avoid duplicate sequencing of accessions on the SGN SOL-100 page http://solgenomics.net/organism/sol100/view. This topic is for the discussion of SOL-100 related questions.
Started by:
wendy wilson
2021-11-29 06:42:02
 
Polymerase chain reaction (PCR) represents a rapid, sensitive, and specific method for in vitro amplification of nucleic acid sequences. By using specific oligodeoxynucleotide primers and DNA polymerases, the PCR is able to identify a target sequence and then amplify millions of copies of the target.

https://histobiolab.com/pcr-and-quantitative-pcr.html
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Started by:
Joe Cohen
2021-11-28 16:40:59
 
Mitochondrial whole-genome sequencing uses a high-throughput sequencing platform for mitochondrial whole-genome sequencing of different individuals or groups and bioinformatic analysis at the individual or population level. Mitochondrial whole-genome sequencing can fully explore the genetic variation of mtDNA level, and provide important information for screening disease pathogenic and susceptible genes, studying disease genetic mechanism, evolution, and population genetics.

https://www.mitochondriasci.com/mitochondrial-whole-genome-sequencing.html
Started by:
Isaak Tecle
2021-11-28 09:38:56
 
Started by:
Mia Chou
2021-11-27 18:33:34
 
Lipopolysaccharide (LPS) is a natural adjuvant synthesized by gram-negative bacteria. LPS and its lipid A moiety can stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

https://www.creative-biogene.com/lipopolysaccharides-from-e-coli-eh100-item-var-007-470716.html
The plant biotechnology and molecular breeding laboratory at Mid-Florida Research & Education Center at the University of Florida is seeking a highly motivated scientist to conduct studies of the environmental effect on tomato seed development, which is sponsored by FFAR for three years. The objective of this project is to identify the small RNAs that regulate seed development in response to maternal temperatures. This position requires a person with horticultural practices in growing tomato plants and a strong background in small RNA sequencing and analysis. The majority of research activities will be completed at Mid-Florida Education and Research Center, which is about 15miles from Orlando downtown, Florida; occasional travel to other states such as California may be required.

Applicants should receive a Ph.D. degree within the past 5 years with a solid background in bioinformatics and molecular biology/plant biotechnology, and have at least one first-authored publication in a peer-reviewed journal. Expertise in plant molecular techniques (small RNA delivery, siRNAs manipulation, CRISPR gene-editing) and research experiences in tomato or seed biology will be highly preferred. Candidates should have excellent skills in communications in oral and written English, skills in critical thinking, and are able to work independently in a culture-diverse environment. Applicants with legal visa status in the U.S. will be seriously considered. Salary is competitive and commensurate with background and experience($48000 plus medical benefit etc.). This position will be immediately available and remain open until filled. The term for this position is one year, and renewable contingent on academic performance for at least another 2 years. Qualified applicants should send a CV, and contact information for 3-4 references by email to hhuo@ufl.edu.
Started by:
wendy wilson
2021-11-21 11:23:35
 
Chromosome analysis is usually a helpful test to determine the genetic contribution to a constellation of medical and/or developmental problems. In addition, stem cells in culture can accumulate changes including chromosomal abnormalities. Alterations in the genome can lead to changes in gene expression and cellular functions, increasing the risk of stem cell tumorigenesis. Therefore, it is crucial to monitor a culture for any chromosomal abnormalities, particularly in stem cells intended for therapeutic use.

https://histobiolab.com/chromosome-analysis.html
Started by:
Peng Wang
2021-11-19 05:32:40
 
Here we report a serious problem with tobacco gene models. We tried to map the gene models of Nitociana tabacum (TN90), which you provide in your ftp, to the TN90 genome assembly sequences. Unfortunately, a lot of gene models could not be mapped. Of the 122388 gene models you provided in the gene model file,only 53714 could be mapped to the genome assembly. We do not know what method you used to identify gene models. Could you check all the datasets of the three tobacco varieties(TN90, K326 and Bathma)? Thank you.
Started by:
Mia Chou
2021-11-17 09:41:10
 
shRNA is a sequence of RNA which makes a tight hairpin turn that can be used to silence target gene expression via RNAi. Expression of shRNA in cells is typically accomplished by delivery of plasmids or through viral or bacterial vectors. Compared to siRNA, shRNA offers benefits in silencing longevity, delivery options, and cost. Expressed shRNA is transcribed in cells from a DNA template as a single-stranded RNA (ssRNA) molecule (~50 – 100 bases). Complementary regions spaced by a small 'loop' cause the transcript to fold back on itself forming a 'short hairpin' in a manner analogous to natural microRNA. Recognition and processing via the RNAi machinery convert the shRNA into the corresponding siRNA.

https://www.creative-biogene.com/products/shrna-clones.html
Started by:
john binns
2021-11-17 06:23:30
 
This topic is for members of the SOL community from around the world to publish job openings. If interested, please reply directly to the poster. Job postings submitters please note that the postings will be removed by default 8 months after posting.
This topics is for the researchers of the SOL community around the world who need some specific materials for their experiments and express some request to those who could provide them with the expected materials or resources.
Started by:
Mia Chou
2021-11-15 15:15:19
 
RNA interference (RNAi) is a conserved biological response to the presence of double-stranded RNA, which mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes. Due to its characteristics, researchers have used RNAi as a tool to selectively study the function of genes, and with the advent of genome-wide or gene set libraries of small interfering RNAs (siRNAs) targeting different organisms, its application is now possible in a truly systematic way.

https://www.creative-biogene.com/products/sirna-libraries.html
Started by:
Amir Tavassoli
2021-11-15 04:53:22
 
Dear Sir/Madam
Hi
I need all of tomato reference and integrated maps such as "Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589".
But when I choose the map, I see "Server error" message.
Please let me know how can I get these maps.
Regards.
Hi, I would like to know if there is a straightforward way to translate the one nomenclature to the other.
Both refer to genes/loci/protein, right... but if I have to compare to see coincidences from one list in one format and one list in the other (having hundreds of proteins/genes in each list), I can die doing searches and endless alignments, and still not accomplishing the job...
Please help.
Thank you very much.
Started by:
Douglas Maxwell
2021-11-10 00:43:07
 
Started by:
Erwin Aragon
2021-11-10 00:38:34
 
Dears friends: I want to Know if Are there microsatellite or other molecular marker that can be used for identify differents species in tomato??? i mean between sculentum and
Started by:
Mia Chou
2021-11-07 14:59:34
 
Coronaviruses (CoVs) are a large family of viruses that cause human diseases, including severe acute respiratory syndrome coronavirus (SARS-CoV) which causes severe acute respiratory syndrome (SARS), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), previously known as 2019 novel coronavirus (2019-nCoV) which leads to coronavirus disease 2019 (COVID-19).

Angiotensin converting enzyme 2, also known as ACE2, is an exopeptidase that catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 receptor may play roles in the regulation of renal and cardiovascular function, as well as fertility. It is mainly expressed in vascular endothelial cells of the heart and the kidneys. Studies show that ACE2 receptor is the entry point into human cells for some coronaviruses, including the SARS virus and SARS-CoV-2 (previously known as 2019-nCoV).

https://www.creative-biogene.com/products/coronavirus-receptor-stable-cell-lines.html
Started by:
optimus optimus
2021-11-07 14:58:46
 
Hi,
I am very confused browsing literature because I can not find a clear statement about the ploidy of N. benthamiana.
N. tabacum is clearly an allotetraploid, and tetraploid (2n = 4x = 48), with 4 copies of n=12. Genome size over 4.5 Gb, however,
N benthamiana it is reported to be an "ancient" allotetraploid, but currently it is n=19, and 38 total number of chromosomes, so it seems that from the ancient allotetraploid (48 chromosomes), genome fractionation has occurred and 38 remains, and that currently the genome is "diploid" (2n = 2x = 38). Is this correct?. Is N benthamiana diploid?.
Thank you very much.
Best regards,
Started by:
Mia Chou
2021-11-07 14:55:41
 
As useful tools for scientific research, reporter stable cell lines are those cells involved in expression of reporter protein(s) which are engineered through integrating the reporter expression cassettes into cell genomes mediated by plasmid or lentivirus vectors. In the studies of life science and biotechnology, the commonly used reporter genes are visually identifiable including green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, secreted alkaline phosphatase (SEAP) etc.

https://www.creative-biogene.com/products/reporter-stable-cell-lines.html
Is it safe to directly map the gene identifiers (Solyc00g005000.2) using the primary id (Solyc00g005000)?
Started by:
jonny sins
2021-10-21 14:37:08
 
Yes, essay writing services area unit completely legal to use. If you would like to shop for essays or get your assignment done by consultants, doing thus from an internet essay writing service is totally legal. These services are around for several years serving to students with their school courses.
I would like to know whether Triticum aestivum database is updated and is there any reference where I researchers have designed construct for VIGS in wheat
2021-10-01 05:19:15
 
Hi dears,
Is there any possibility that someone can share the cDNA library of benthamiana? Please let me know, if it is possible. Thanks.
Started by:
Sridhar Gutam
2021-09-08 11:37:17
 
Dear All,

I would like to work on phenotyping tomato and brinjal (egg plant) for abiotic stress tolerance traits. For that I would like to know what are the materials and methods needed.

Why I am asking is that if any one in the community had developed a set of protocols for low cost phenotyoing, i would like to use it.

Please connect me with people who are working on phenotyping tomato.

Thanks
Sridhar
Hello
I would like to access the potato annotation file in iTAG nomenclature with their respective TAIR nomenclature codes equivalent to each gene. Is that possible? I can not find the annotation file that includes that information. I need to match the nomenclature accepted in DAVID (The Database for Annotation).

Example in PGSC annotation available in Phytozome

PGSC0003DMG400000001 = AT1G12600.1
Started by:
Lukas Mueller
2021-05-18 10:57:36
 
This topic contains release notes for new SGN data releases.
Started by:
Emilio Ortiz
2014-01-19 23:11:32
 
Hi,

Is there any way to download the 2860 COSII sequences? Please let me know.


Thank you,
Started by:
Eva Huala
2012-02-14 14:26:45
 
Started by:
Lukas Mueller
2009-07-23 12:10:22
 
This topic contains release notes for new SGN website features.
Started by:
Isaak Tecle
2008-06-03 15:53:13