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Targeted metabolomics is not only an important part of metabolomics research, but also an extension of total metabolome research. Compared with total metabolome analysis, targeted metabolome analysis has several characteristics such as strong specificity, high detection sensitivity and accurate quantification.

https://www.microbialtec.com/targeted-metabonomics.html
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Microbial fermentation is divided into aerobic fermentation and anaerobic fermentation according to the requirements of fermentation conditions. The microbes commonly used in industrial production are mainly bacteria, actinomycetes, fungi and yeasts. Due to the development of fermentation engineering itself and the intervention of genetic engineering, algae and viruses are gradually becoming microbes for industrial production.

https://www.microbialtec.com/microbial-fermentation.html
Voltage-gated chloride channels display a variety of important physiological and cellular roles that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Based on sequence homology the chloride channels can be subdivided into a number of groups.

https://acroscell.creative-bioarray.com/chloride-channels.html
Creative Biogene is a leading biotechnology company offering a suite of trusted services and solutions for identification, classification, quantitation and functional analysis of microbes in complex biological mixtures. Whether you are performing sequencing for discovery, characterization, or routine microbial identification, Creative Biogene technologies provide versatile approaches to meet your needs. Our company specializes in microbial identification as well as custom sequencing project services with highly competitive prices and fast turnaround times. With talented stuffs and over years of experience, we can totally satisfy your project requirements and budgets in the exploration of microbial biodiversity.

https://microbiosci.creative-biogene.com/dna-sequencing-16s-18s-its.html
Hi,
I am very confused browsing literature because I can not find a clear statement about the ploidy of N. benthamiana.
N. tabacum is clearly an allotetraploid, and tetraploid (2n = 4x = 48), with 4 copies of n=12. Genome size over 4.5 Gb, however,
N benthamiana it is reported to be an "ancient" allotetraploid, but currently it is n=19, and 38 total number of chromosomes, so it seems that from the ancient allotetraploid (48 chromosomes), genome fractionation has occurred and 38 remains, and that currently the genome is "diploid" (2n = 2x = 38). Is this correct?. Is N benthamiana diploid?.
Thank you very much.
Best regards,
Hi dears,
Is there any possibility that someone can share the cDNA library of benthamiana? Please let me know, if it is possible. Thanks.
Tumor immunology is a science that uses the theory and methods of immunology to study the antigenicity of tumors, the relationship between the body's immune function and tumors, the body's immune response to tumors and its anti-tumor immune mechanism, tumor immunodiagnosis and immune prevention and treatment.

https://histobiolab.com/immuno-oncology-services.html
Phospholipid fatty acids (PLFA) are key components of microbial cellular membranes from bacteria to plants and animals. PLFA analysis is a technique widely used for estimation of the total microbial biomass and community composition of the living microbiota in soils, drinking waters, groundwaters, sediments and biomats. Creative Biogene is a leading company offering the high-quality PLFA analysis services.

https://microbiosci.creative-biogene.com/phospholipid-fatty-acid-analysis.html
This topic discusses issues in developing controlled-vocabularies for describing Solanaceae phenotypes and traits.
Most should be covered by the 'Solanaceae Phenotype Ontology' (SPO)
(see SGN->Tools->Ontology browser) which is growing by demand of its users community.
Terms which exist in other ontologies (PO, PATO, etc) are mapped to SPO terms.
In some cases it will be advised to use other vocabularies, instead of providing new SPO terms.
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When designing IHC/ICC experiments, the choice of primary antibody is very important. All steps of the IHC/ICC experiment must be optimized in order to observe specific staining and minimize non-specific background staining. This includes conducting preliminary experiments to determine the appropriate incubation conditions for each primary antibody. In order to obtain reliable and specific signals, high-quality antibodies with minimal cross-reactivity should be used.

https://histobiolab.com/antibody-selection-and-optimization.html
Untargeted metabolomics, also known as discovery metabolomics, is mainly used to compare the metabolomes (all metabolites of a certain organism) of the control group and the experimental group to find the differences in their metabolites.

https://www.microbialtec.com/untargeted-metabonomics.html
I would like to know whether Triticum aestivum database is updated and is there any reference where I researchers have designed construct for VIGS in wheat
Multilocus Sequence Typing (MLST) is a molecular biological approach for typing based on multiple loci sequencing to fulfill the identification and classification of certain microbes. Since first proposed by Maiden in 1998 for the analysis of natural variation of Neisseria meningitides, MLST database has been developing and supplementing over the past two decades, this approach is widely used as a portable tool for the identifying clonal relationships among multiple pathogens, environmental bacteria or eukaryotes.

https://microbiosci.creative-biogene.com/multilocus-sequence-typing-service.html
RNA-seq can be used for unbiased strand specific identification of both common and novel transcripts. The wide dynamic range allows you to reliably identify high and low expression in microbial RNA-seq experiments. Multiple samples can be processed at once with a simplified workflow suitable for automation.

https://www.microbialtec.com/microbial-rna-sequencing.html
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In microbial DNA sequencing, because of the large number of species, traditional sequencing methods cannot be used to solve the microbial problems. Creative Biogene has many sequencing methods based on a professional high-throughput platform, and provides you with a full range of microbial DNA sequencing solutions.

https://www.microbialtec.com/microbial-dna-sequencing.html
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ATP-gated P2X receptor cation channel family , or simply P2X receptor family, consists of cation-permeable ligand-gated ion channels that open in response to the binding of extracellular adenosine 5'-triphosphate. They belong to a larger family of receptors known as the ENaC/P2X superfamily. ENaC and P2X receptors have similar 3-D structures and are homologous. P2X receptors are present in multiple organisms including humans, mouse, rat, rabbit, chicken, zebrafish, bullfrog, fluke, and amoeba.

https://acroscell.creative-bioarray.com/atp-gated-p2x-channels.html
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CRISPR/Cas9 Platform offers one-stop knockout cell line generation service for our clients. Our custom cell line service begins with free consultation in which we will run a feasibility analysis for each case. Once the project begins, we will work closely with you and regularly update the project progress.

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In situ hybridization is a technique for detecting the expression pattern of a particular gene sequence in cells or tissues using a complimentary sequence known as a probe. The probe is typically labeled with a fluorochrome or an enzyme, so it can be detected either by using a fluorescent microscope or with a bright field microscope.

https://histobiolab.com/in-situ-hybridization-ish.html
CRISPR/Cas9 Platform offers a one-stop plant genome editing service, including gene knockout, gene knockin and gene deletion. Various species are available for this service, including but not limited to Arabidopsis thaliana, Zea mays, rice and tobacco. With excellent platform, our experienced scientists are dedicated to providing a reliable and satisfied service for our customers.

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Dear Sol genomics network,

Shin-Han Shiu, Marja Timmermans, and I are organizing this summer's Cold Spring Harbor "Frontiers and Techniques in Plant Sciences" (June 27 - July 17).

The course provides foundational work in plant sciences as well as practical laboratory experience in techniques like RNA-seq, proteomics, mapping-by-sequencing, genome editing, and imaging. In recent years the course has stressed the quantitative nature of plant biology and students will be introduced to computational techniques required for analyzing large datasets.

As you may know, this course has been going strong for >30 years and has been taken by many successful plant scientists. I encourage you to let students, postdocs and faculty colleagues know about the course, which will be taught be a fantastic group of investigators http://meetings.cshl.edu/courses/2014/c-plan14.shtml.

CSH can provide limited financial support for US students to cover their tuition room and board through an NSF grant and other sources of funding. Thus, I strongly encourage you to consider sending your new students and/or post-docs to the course, which has had a very strong positive impact on the trajectory of participants.

APPLICATIONS ARE DUE APRIL 15.

Thank you,

Mark Johnson
Brown University
CRISPR/Cas9 Platform offers gene knockout mouse generation services for our clients. With experienced scientists and excellent gene editing platform, our staff has successfully generated various gene KO mice by using CRISPR/Cas9. We provide a one-stop KO mouse generation service that covers from sgRNA design and construct, pronuclear microinjection to Founders genotyping and breeding. We are dedicated to utilize the latest technology to service our customers for achieving their goals.

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Creative Bioarray has various organizations from various animal species. Our team has many years of comprehensive experience in TCR research, and has successfully optimized IHC analysis for a variety of new antibody types. Relying on an advanced histology technology platform and a professional technical team, we can provide you with all the services you need for TCR research.

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Musculoskeletal diseases include more than 150 confirmed conditions that affect the motor system. They range from short-lived problems (such as fractures, sprains, and strains) to lifelong conditions such as persistent pain and disability. The typical characteristics of musculoskeletal disorders are pain (usually persistent pain) and limited mobility and function.

https://histobiolab.com/musculoskeletal-pathology-services.html
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Voltage-gated potassium channel plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient.

https://acroscell.creative-bioarray.com/potassium-channels.html
CRISPR/Cas9 technology has been used as a genome editing tool for a variety of cell lines, including a variety of tumor cells in humans and mice. In addition, based on its easy operation and high accuracy, CRISPR/Cas9 technology have great potential for application in vitro disease models and disease treatment.

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Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Immunofluorescence (IF) all utilize antibodies to provide visual details about protein abundance, distribution, and localization.

https://histobiolab.com/ihc-icc-if-staining.html
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Creative Bioarray has various organizations from various animal species. Our team has many years of comprehensive experience in TCR research, and has successfully optimized IHC analysis for a variety of new antibody types. Relying on an advanced histology technology platform and a professional technical team, we can provide you with all the services you need for TCR research.

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Creative Biogene is a leading company offering the high-quality microbial identification services. Polyphasic approach for bacterial classification such as DNA sequencing(16S/18S/ITS) integrated with fatty acid analysis become important tools in microbial systematics. Occasionally fatty acid analysis or DNA sequencing(16S/18S/ITS) can’t individually produce a definitive identification. With a combined genotypic, chemotaxonomic and phenotypic approach, polyphasic analysis provides you with the most thorough and reliable results.

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When designing IHC/ICC experiments, the choice of primary antibody is very important. All steps of the IHC/ICC experiment must be optimized in order to observe specific staining and minimize non-specific background staining. This includes conducting preliminary experiments to determine the appropriate incubation conditions for each primary antibody. In order to obtain reliable and specific signals, high-quality antibodies with minimal cross-reactivity should be used.

https://histobiolab.com/antibody-selection-and-optimization.html
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CRISPR/Cas9 PlatformCB utilizes CRISPR/Cas9 technology to generate custom genome-editing animal models for customers. Various species are available, including mouse, rat, zebrafish, C.elegans. CRISPR/Cas9 PlatformCB brings customer a wide range of genetic alterations service, including knockout, point mutations, short insertions, gene knock-in and conditional knock-in. In terms of experienced scientists, skilled staff and excellent platform, we provide one-stop custom animal models generation service with high success ratio.

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Dear friends, I am about to begin an investigation of lycopene content in tomato species, as well as identification of microsatellites to identify the gene or genes responsible for the character under study, so I ask if I could help with information on the research topic . Thank you very much
Dear all,
I need your help here. Does anyone know how to convert ITAG gene tomato annotations to KEGG (or UniProt) ID?

Thanks in advance!

Irene
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Transient receptor potential channels (TRP) are non-selective calcium permeant cation channel located mostly on the plasma membrane of numerous animal cell types. They are involved in osmotic sensitivity and mechano-sensitivity. The exposure to hypotonicity within the physiological range induces some TRP channels activation exhibiting an outward rectification. TRP channels can also be activated by heat, low pH, citrate and phorbol esters.

https://acroscell.creative-bioarray.com/trp-channels.html
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As a molecular technology, PCR facilitates the amplification of specific nucleic acid sequences to produce a large number of amplified products that can be analyzed by other methods. Therefore, the PCR offers a very sensitive and specific method for the quantitative and qualitative analysis of target sequences. The development of various chemistries for primer and probe labeling has produced an extraordinary technology in terms of performance characteristics.

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Calcium channel have been well recognized as therapeutic targets for treating a number of diseases. However, the calcium channel class is considered to be underexploited due to the lack of reliable functional cell-based assays for high-throughput screening.

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Creative Biogene is a leading company offering the high-quality matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) services. Mass spectrometry (MS)-based technologies, particularly MALDI-TOF, has emerged as a potential tool for rapid, sensitive, accurate, and cost-effective microbial identification of common as well as esoteric bacteria and fungi. MALDI-TOF MS process is automated and high throughput, and higher taxonomic resolution than either 16S/18S rRNA gene sequence analysis. Our talented scientists have tremendous experience to offer reliable service aiming to characterize microbial species. We guarantee a quick turnaround-time, competitive pricing.

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Started by:
john binns
2021-09-16 06:14:04
 
This topic is for members of the SOL community from around the world to publish job openings. If interested, please reply directly to the poster. Job postings submitters please note that the postings will be removed by default 8 months after posting.
Started by:
Ava Davis
2021-09-13 22:21:25
 
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Started by:
Amir Tavassoli
2021-09-10 12:27:46
 
Dear Sir/Madam
Hi
I need all of tomato reference and integrated maps such as "Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589".
But when I choose the map, I see "Server error" message.
Please let me know how can I get these maps.
Regards.
Started by:
Sridhar Gutam
2021-09-08 11:37:17
 
Dear All,

I would like to work on phenotyping tomato and brinjal (egg plant) for abiotic stress tolerance traits. For that I would like to know what are the materials and methods needed.

Why I am asking is that if any one in the community had developed a set of protocols for low cost phenotyoing, i would like to use it.

Please connect me with people who are working on phenotyping tomato.

Thanks
Sridhar
Started by:
Isaak Tecle
2021-09-07 08:10:21
 
Started by:
mac ali
2021-09-04 15:06:12
 
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This topics is for the researchers of the SOL community around the world who need some specific materials for their experiments and express some request to those who could provide them with the expected materials or resources.
Started by:
Erwin Aragon
2021-09-03 06:14:24
 
Dears friends: I want to Know if Are there microsatellite or other molecular marker that can be used for identify differents species in tomato??? i mean between sculentum and
Started by:
Peng Wang
2021-09-01 03:04:31
 
Here we report a serious problem with tobacco gene models. We tried to map the gene models of Nitociana tabacum (TN90), which you provide in your ftp, to the TN90 genome assembly sequences. Unfortunately, a lot of gene models could not be mapped. Of the 122388 gene models you provided in the gene model file,only 53714 could be mapped to the genome assembly. We do not know what method you used to identify gene models. Could you check all the datasets of the three tobacco varieties(TN90, K326 and Bathma)? Thank you.
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2021-08-29 14:32:19
 
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Lukas Mueller
2021-08-18 07:53:22
 
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Peter Nilson
2021-08-18 05:36:15
 
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Started by:
Emilio Ortiz
2021-08-17 18:30:01
 
Hi,

Is there any way to download the 2860 COSII sequences? Please let me know.


Thank you,
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Peter Nilson
2021-08-13 16:45:41
 
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2021-08-13 16:41:19
 
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The plant biotechnology and molecular breeding laboratory at Mid-Florida Research & Education Center at the University of Florida is seeking a highly motivated scientist to conduct studies of the environmental effect on tomato seed development, which is sponsored by FFAR for three years. The objective of this project is to identify the small RNAs that regulate seed development in response to maternal temperatures. This position requires a person with horticultural practices in growing tomato plants and a strong background in small RNA sequencing and analysis. The majority of research activities will be completed at Mid-Florida Education and Research Center, which is about 15miles from Orlando downtown, Florida; occasional travel to other states such as California may be required.

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jamatrauon jain
2021-07-27 15:21:15
 
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Timothy Shafer
2021-07-27 10:38:43
 
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I would like to access the potato annotation file in iTAG nomenclature with their respective TAIR nomenclature codes equivalent to each gene. Is that possible? I can not find the annotation file that includes that information. I need to match the nomenclature accepted in DAVID (The Database for Annotation).

Example in PGSC annotation available in Phytozome

PGSC0003DMG400000001 = AT1G12600.1
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2021-07-21 09:12:34
 
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2021-07-20 12:27:36
 
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Is it safe to directly map the gene identifiers (Solyc00g005000.2) using the primary id (Solyc00g005000)?
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Started by:
Lukas Mueller
2021-05-18 10:57:36
 
This topic contains release notes for new SGN data releases.
Hi, I would like to know if there is a straightforward way to translate the one nomenclature to the other.
Both refer to genes/loci/protein, right... but if I have to compare to see coincidences from one list in one format and one list in the other (having hundreds of proteins/genes in each list), I can die doing searches and endless alignments, and still not accomplishing the job...
Please help.
Thank you very much.
Started by:
Eva Huala
2012-02-14 14:26:45
 
Started by:
Lukas Mueller
2011-12-14 10:49:32
 
In the next few years, hundreds of Solanaceae will be sequenced using next generation sequencing methods. If you intend to sequence a Solanaceae, please provide information on the species to avoid duplicate sequencing of accessions on the SGN SOL-100 page http://solgenomics.net/organism/sol100/view. This topic is for the discussion of SOL-100 related questions.
Started by:
Douglas Maxwell
2008-07-11 21:24:26
 
Started by:
Isaak Tecle
2008-06-03 15:53:50