1. Files used for primer template: SL2.30chxx.repeats.softmasked.stringent.itag0 - genomic sequence. 2. Gaps greater than or equal to 20 base pairs were selected as targets. 3. Predicted PCR product sizes are between 60 to 10,000 bp. 4. Files ending in .p3out are primer3 output 5. Files ending in .list contain a list of primers predicted to flank gaps. -oripos refers to the gap position in the original genomic sequence file -allelepos refers to the gap position in the sequence fragment used for primer3 input.