The unigene_build ftp directory are divided in two parts: 1- single_species_assemblies, where there are one folder per single species used in the unigene build. Currenly the species used are: - Antirrhinum_majus - Capsicum_annuum - Coffea_canephora - Ipomoea_batatas - Nicotiana_benthamiana - Nicotiana_sylvestris - Nicotiana_tabacum - Petunia_hybrida - Solanum_lycopersicum - Solanum_melongena - Solanum_tuberosum 2- combined_species_assemblies, where there are unigene assemblies for species close related. Currently the files stored are: - all_sgn_unigenes - Hedyotis_species - tomato_species All these folders are organized in: 3 files, with the most recient unigene build version: .seq, with the unigene sequences in fasta format. .qual, with the qscores produced in the assembly in fasta format. .membermapping.tab with the mapping between unigenes and ests. It have 7 columns: -Unigene SGN accession, -EST SGN accession, -consensus_start_position, -consensus_end_position, -member_start_position, -member_end_position -orientation (+ => direct) (- => reverse) If there are more than one unigene_build version, there are also another file .versionmapping.tab with the mapping between different unigene build versions with the EST accessions in the first column and the unigenes accessions in the next. curr/ folder with a link to the most recient unigene build version old/ folder with the old unigene build versions Note1: We are reorganizing the ftp site, so if you find an error or you think that could be a good idea add new files related with the unigene_builds (as some annotation files), please contact with us at: sgn-feedback[at]sgn.cornell.edu Note2: We are working to add annotation files in tab format for these unigene builds. Note3: The protocols used to build these unigene_builds will be available soon too.