GeneSeqer. Version of March 12, 2006. Date run: Mon Aug 28 21:31:01 2006 (Bayesian) Splice site model (species): Arabidopsis thaliana Fast search parameters: MinMatchLen 16, MinQualityHSP 30, MinQualityCHAIN 50. Total number of ESTs: 34829 Total sequence length: 35392868 Minimum sequence length: 63 Maximum sequence length: 5381 Length distribution (number of sequences of specified length): < 100: 4 < 200: 53 < 300: 143 < 400: 353 < 500: 791 < 600: 1674 < 700: 2583 < 800: 4023 < 900: 6481 < 1000: 5706 >=1000: 13018 Input file : /tmp/bac-submission-temp-SSV3m/C06HBa0016K14/C06HBa0016K14.seq.screen ________________________________________________________________________________ Sequence 1: C06HBa0016K14.1-1, from 1 to 3341, both strands analyzed. ... started at: Mon Aug 28 21:40:47 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 1 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 2 ******************************************************************************** EST sequence 3 -strand 911 n (File: SGN-U328267-) 1 TTTTTTTTTT CTCATAAACA TAAAGTACAC TAATATTATT ATTATAAAAT TCTCCAGTCT 61 TATACAAAAC AACATATGAC TTCACTTGAA CTATAATTAA AGAACAATAA AGGGATAATG 121 CACAAGTACC CCCTCAACCT ATGCCCGAAA TTCCAGAGAC ACACTTATAC TATACTAAGG 181 TCCTATTACC CCCCTGAACT TATTTTATAA GTAATTTTCT ACCCCTTTTT AGCCTACGTG 241 GCACTAGTTT AAAAAAAAAG TCAACAACCA TTGGGCCCAC AAGATAGTGC CACGTAGGTC 301 TAAAAGGGGT AAAAAATTAT TAATAAATAA GTTCAGGGGG TAATAAGATC TTAGTATGGT 361 ATAAGTGTAT CTCTGAGATT TTGGACATAG GCTAAGGGGG TACTTGGACA TTATCCCAAC 421 AATAAATAAA GGATATAACA TGATTCAAAA GACAACACGT GATAACCACT TCTACAACTT 481 GTGCATGATC AATTGAGCAC CATATTGAGG TTGAAGCAAC AATGGGTGAG GAGCATGAGC 541 ATAAGATGGA GAGAGTTCAA ATGCATAATG TTTTAGAATC ATGACCATTG CCATTTTTGC 601 CTCTAACATA GCAAAATTTT GCCCAATACA TATTCTTGGA CCCCAACTAA ATGGAAAAAA 661 TACAACTTGT CCTTTTGTTG CTTTTGATAT TCCTTCACTA AATCTCTCTG GCATAAACTC 721 CATTGCATCA TCTCCCCATA TTTCAGTATC ATGATGCACT AACATTGTTG CCAATATGAG 781 TTGGACCCCA GAGGGTAAAC ACAAATCCCC TAACTTTGTT TCTGTATTCA CCATGCGATT 841 AATCGCGTAT ACTGATGTAT ACAACCTTAA GACCTCGTTT AAGATCATTG TAACCACTTT 901 TAGTTGATTC A Predicted gene structure (within gDNA segment 1 to 3341): Exon 1 1100 1411 ( 312 n); cDNA 108 417 ( 310 n); score: 0.840 PPA cDNA 12 1 MATCH C06HBa0016K14.1-1+ SGN-U328267- 0.840 312 0.342 C PGS_C06HBa0016K14.1-1+_SGN-U328267- (1100 1411) Alignment (genomic DNA sequence = upper lines): TAAAAGAAAA AATGTACGAG TATCCCCTTA ATCTATGTCC AAAATTCCAG AGACACACTT 1159 |||| | | |||| || || || ||||| | | ||||| || ||||||||| |||||||||| TAAAGGGAT- AATGCACAAG TACCCCCTCA ACCTATGCCC GAAATTCCAG AGACACACTT 166 ATACTATACT AAGATCTTAT TA--CCCCTG AACTTATTTT ATAAGTAATT TTCTACCCCT 1217 |||||||||| ||| || ||| || |||||| |||||||||| |||||||||| |||||||||| ATACTATACT AAGGTCCTAT TACCCCCCTG AACTTATTTT ATAAGTAATT TTCTACCCCT 226 TTTGGGCCTA CGTGGCACTA GCTTGAAAAA AAGAATCAAC CAACGTTGCA CCCACAAGAT 1277 ||| ||||| |||||||||| | || ||||| || | ||||| | | ||| |||||||||| TTTTAGCCTA CGTGGCACTA G-TTTAAAAA AAAAGTCAAC AACCATTGGG CCCACAAGAT 285 AGTGTCACGT AGGCCGAAAA GGGGTAGAAA ATTATTAATA AAATAAGTTC AGGATTGTAA 1337 |||| ||||| ||| | |||| |||||| ||| ||||||||| |||||||||| ||| |||| AGTGCCACGT AGGTCTAAAA GGGGTAAAAA ATTATTAAT- AAATAAGTTC AGG-GGGTAA 343 TAAAACCTTA GTATAGTATA AGTGTTTCTC TGAAATTTCG GACATAGATT GAGGGGATAG 1397 ||| | |||| |||| ||||| ||||| |||| ||| |||| | ||||||| | ||||| || TAAGATCTTA GTATGGTATA AGTGTATCTC TGAGATTTTG GACATAGGCT AAGGGGGTAC 403 TTGTGCATTA TCCC 1411 ||| ||||| |||| TTGGACATTA TCCC 417 hqPGS_C06HBa0016K14.1-1+_SGN-U328267- (1100 1411) ******************************************************************************** EST sequence 1 +strand 911 n (File: SGN-U328267+) 1 TGAATCAACT AAAAGTGGTT ACAATGATCT TAAACGAGGT CTTAAGGTTG TATACATCAG 61 TATACGCGAT TAATCGCATG GTGAATACAG AAACAAAGTT AGGGGATTTG TGTTTACCCT 121 CTGGGGTCCA ACTCATATTG GCAACAATGT TAGTGCATCA TGATACTGAA ATATGGGGAG 181 ATGATGCAAT GGAGTTTATG CCAGAGAGAT TTAGTGAAGG AATATCAAAA GCAACAAAAG 241 GACAAGTTGT ATTTTTTCCA TTTAGTTGGG GTCCAAGAAT ATGTATTGGG CAAAATTTTG 301 CTATGTTAGA GGCAAAAATG GCAATGGTCA TGATTCTAAA ACATTATGCA TTTGAACTCT 361 CTCCATCTTA TGCTCATGCT CCTCACCCAT TGTTGCTTCA ACCTCAATAT GGTGCTCAAT 421 TGATCATGCA CAAGTTGTAG AAGTGGTTAT CACGTGTTGT CTTTTGAATC ATGTTATATC 481 CTTTATTTAT TGTTGGGATA ATGTCCAAGT ACCCCCTTAG CCTATGTCCA AAATCTCAGA 541 GATACACTTA TACCATACTA AGATCTTATT ACCCCCTGAA CTTATTTATT AATAATTTTT 601 TACCCCTTTT AGACCTACGT GGCACTATCT TGTGGGCCCA ATGGTTGTTG ACTTTTTTTT 661 TAAACTAGTG CCACGTAGGC TAAAAAGGGG TAGAAAATTA CTTATAAAAT AAGTTCAGGG 721 GGGTAATAGG ACCTTAGTAT AGTATAAGTG TGTCTCTGGA ATTTCGGGCA TAGGTTGAGG 781 GGGTACTTGT GCATTATCCC TTTATTGTTC TTTAATTATA GTTCAAGTGA AGTCATATGT 841 TGTTTTGTAT AAGACTGGAG AATTTTATAA TAATAATATT AGTGTACTTT ATGTTTATGA 901 GAAAAAAAAA A Predicted gene structure (within gDNA segment 1 to 3341): Exon 1 1110 1432 ( 323 n); cDNA 500 821 ( 322 n); score: 0.777 PPA cDNA 900 911 MATCH C06HBa0016K14.1-1+ SGN-U328267+ 0.777 323 0.355 C PGS_C06HBa0016K14.1-1+_SGN-U328267+ (1110 1432) Alignment (genomic DNA sequence = upper lines): AATGTACGAG TATCCCCTTA ATCTATGTCC AAAATTCCAG AGACACACTT ATACTATACT 1169 ||||| | || || ||||||| |||||||| ||||| ||| ||| |||||| |||| ||||| AATGTCCAAG TACCCCCTTA GCCTATGTCC AAAATCTCAG AGATACACTT ATACCATACT 559 AAGATCTTAT TA-CCCCTGA ACTTATTTTA TAAGTAATTT TCTACCCCTT TTGGGCCTAC 1228 |||||||||| || ||||||| ||||| |||| | | |||||| | |||||||| || | ||||| AAGATCTTAT TACCCCCTGA ACTTA-TTTA TTAATAATTT TTTACCCCTT TTAGACCTAC 618 GTGGCACTAG CTTGAAAAAA AGAATCAACC AACGTTGCAC CCACAAGATA GTGTCACGTA 1288 ||||||||| |||| ||| | || || ||| |||||| GTGGCACTAT CTTG-TGGGC CCAATGGTTG TTGACTTTTT TTTTAAACTA GTGCCACGTA 677 GGCCGAAAAG GGGTAGAAAA TTATTAATAA AATAAGTTCA GGATTGTAAT AAAACCTTAG 1348 ||| ||||| |||||||||| ||| | |||| |||||||||| || ||||| | ||||||| GGCTAAAAAG GGGTAGAAAA TTACTTATAA AATAAGTTCA GGGGGGTAAT AGGACCTTAG 737 TATAGTATAA GTGTTTCTCT GAAATTTCGG ACATAGATTG AGGGGATAGT TGTGCATTAT 1408 |||||||||| |||| ||||| | |||||||| ||||| ||| ||||| || | |||||||||| TATAGTATAA GTGTGTCTCT GGAATTTCGG GCATAGGTTG AGGGGGTACT TGTGCATTAT 797 CCCGTT-TTA TCTATCAATG TATAG 1432 ||| || || | | ||| ||||| CCCTTTATTG TTCTTTAAT- TATAG 821 hqPGS_C06HBa0016K14.1-1+_SGN-U328267+ (1110 1432) ******************************************************************************** EST sequence 2 -strand 606 n (File: SGN-U324267-) 1 TAAAAAAAAA AGGGAAGTTG AAACTAGAAA CTAATATGAT AATTATAGTG AAGGAGTACT 61 GAGTAGGATT GTACAATATT AAAGGTTTAA CGAATTTATT GAACCATATA TTAAAAAAAA 121 TAATTTATCG AAACATCAAC ATTAAAGGTT TAACGAATTT ATTGAACCAT ATATTGAAAA 181 AACCAATTTA TCGAAACATC AACCTATTTT TTTTTAGAAG ACCCACCGGA ATTTAGGCTT 241 TCGAGTTTTT CAAATTCTTT TGTTTGTCAT AGTCCACAAA ATTGTTTGGC ATTTGAAAAG 301 GTTGTTCACA ACATTGGAAC TATGATCATG GAAAAACTAC TAAAAAACAC ATCTAATATT 361 TTTATTATTT TTAATATTTC ATTTTATTTT TGAAAATCTT TAATACTTCT TATTTCTTTA 421 ATGTATTCGA GCCAGTATTT ACTGTAATTG AATTACATAT TATGTATTTG ATTTATTTTA 481 TCTGGTCCAT TTTATAATGT ATTCGAGCTA CATATTATGT ATTTAATTTA TATTATAATG 541 TATTCGAGAT ACTCTTTAAT GTATCTGAGC TACAAAAAAA AAAAAAAAAA ACTCGAGACT 601 AGTTCT Predicted gene structure (within gDNA segment 1 to 3341): Exon 1 2804 2923 ( 120 n); cDNA 332 452 ( 121 n); score: 0.704 Intron 1 2924 2953 ( 30 n); Pd: 0.000 (s: 0.70), Pa: 0.000 (s: 0.58) ?? Exon 2 2954 3062 ( 109 n); cDNA 453 562 ( 110 n); score: 0.771 PPA cDNA 572 591 MATCH C06HBa0016K14.1-1+ SGN-U324267- 0.736 229 0.378 C PGS_C06HBa0016K14.1-1+_SGN-U324267- (2804 2923,2954 3062) Alignment (genomic DNA sequence = upper lines): AAAAATTACT GAAATAAACG TCTTATGATT CTCGTAGTTT TAATCCTCCC TTCTATTTCT 2863 ||||| |||| ||| | || ||| || || | || ||| |||| | | || ||||| | AAAAACTACT AAAAAACACA TCTAATATTT TTATTATTTT TAATATTTCA TTTTATTTTT 391 AAAAACCTCT AAGATCCCTT ATTTCTTTAA TATATTCGAG CTACATATTA ATGT-ATTCA 2922 |||| || | || | ||| |||||||||| | |||||||| | | ||| ||| ||| | GAAAATCTTT AATACTTCTT ATTTCTTTAA TGTATTCGAG CCAGTATTTA CTGTAATTGA 451 AGCCAGTATT TAATGTATCC AAGCTACATA CTATGCATTC TATTTATTT- -TATAATGTA 2980 | | | ||| ||| ||||| | || | || A......... .......... .......... .T-TACATAT TATGTATTTG ATTTATTTTA 480 TTCGAGCTAG TTTTTAATGT ATTCGAGCTA CATATTATGT ACTCGATTTG TGTTATAATG 3040 | | | | ||| |||||| |||||||||| |||||||||| | | |||| | |||||||| TCTGGTCCAT TTTATAATGT ATTCGAGCTA CATATTATGT ATTTAATTTA TATTATAATG 540 TATTCGAGAT ACTCTTTAAT GT 3062 |||||||||| |||||||||| || TATTCGAGAT ACTCTTTAAT GT 562 hqPGS_C06HBa0016K14.1-1+_SGN-U324267- (2804 2923,2954 3062) Total number of EST alignments reported: 3 ________________________________________________________________________________ Predicted gene locations (2) in segment 1 to 3341: PGL 1 (+ strand): 1100 1432 AGS-1 (1100 1432) SCR (e 0.840) Exon 1 1100 1432 ( 333 n); score: 0.840 PGS (1100 1411) SGN-U328267- PGS (1110 1432) SGN-U328267+ 3-phase translation of AGS-1 (+strand): . . . . . . 1100 TAAAAGAAAAAATGTACGAGTATCCCCTTAATCTATGTCCAAAATTCCAGAGACACACTT - K K K C T S I P L I Y V Q N S R D T L K R K N V R V S P - S M S K I P E T H L K E K M Y E Y P L N L C P K F Q R H T . . . . . . 1160 ATACTATACTAAGATCTTATTACCCCTGAACTTATTTTATAAGTAATTTTCTACCCCTTT I L Y - D L I T P E L I L - V I F Y P F Y Y T K I L L P L N L F Y K - F S T P F Y T I L R S Y Y P - T Y F I S N F L P L . . . . . . 1220 TGGGCCTACGTGGCACTAGCTTGAAAAAAAGAATCAACCAACGTTGCACCCACAAGATAG W A Y V A L A - K K E S T N V A P T R - G P T W H - L E K K N Q P T L H P Q D S L G L R G T S L K K R I N Q R C T H K I . . . . . . 1280 TGTCACGTAGGCCGAAAAGGGGTAGAAAATTATTAATAAAATAAGTTCAGGATTGTAATA C H V G R K G V E N Y - - N K F R I V I V T - A E K G - K I I N K I S S G L - - V S R R P K R G R K L L I K - V Q D C N . . . . . . 1340 AAACCTTAGTATAGTATAAGTGTTTCTCTGAAATTTCGGACATAGATTGAGGGGATAGTT K P - Y S I S V S L K F R T - I E G I V N L S I V - V F L - N F G H R L R G - L K T L V - Y K C F S E I S D I D - G D S . . . . 1400 GTGCATTATCCCGTTTTATCTATCAATGTATAG V H Y P V L S I N V - C I I P F Y L S M Y C A L S R F I Y Q C I Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (-strand): . . . . . . 1432 CTATACATTGATAGATAAAACGGGATAATGCACAACTATCCCCTCAATCTATGTCCGAAA L Y I D R - N G I M H N Y P L N L C P K Y T L I D K T G - C T T I P S I Y V R N I H - - I K R D N A Q L S P Q S M S E . . . . . . 1372 TTTCAGAGAAACACTTATACTATACTAAGGTTTTATTACAATCCTGAACTTATTTTATTA F Q R N T Y T I L R F Y Y N P E L I L L F R E T L I L Y - G F I T I L N L F Y - I S E K H L Y Y T K V L L Q S - T Y F I . . . . . . 1312 ATAATTTTCTACCCCTTTTCGGCCTACGTGACACTATCTTGTGGGTGCAACGTTGGTTGA I I F Y P F S A Y V T L S C G C N V G - - F S T P F R P T - H Y L V G A T L V D N N F L P L F G L R D T I L W V Q R W L . . . . . . 1252 TTCTTTTTTTCAAGCTAGTGCCACGTAGGCCCAAAAGGGGTAGAAAATTACTTATAAAAT F F F S S - C H V G P K G V E N Y L - N S F F Q A S A T - A Q K G - K I T Y K I I L F F K L V P R R P K R G R K L L I K . . . . . . 1192 AAGTTCAGGGGTAATAAGATCTTAGTATAGTATAAGTGTGTCTCTGGAATTTTGGACATA K F R G N K I L V - Y K C V S G I L D I S S G V I R S - Y S I S V S L E F W T - - V Q G - - D L S I V - V C L W N F G H . . . . 1132 GATTAAGGGGATACTCGTACATTTTTTCTTTTA D - G D T R T F F L L I K G I L V H F F F R L R G Y S Y I F S F Maximal non-overlapping open reading frames (>= 64 codons): none PGL 2 (+ strand): 2804 3062 AGS-1 (2804 2923,2954 3062) SCR (e 0.704 d 0.000 a 0.000,e 0.771) Exon 1 2804 2923 ( 120 n); score: 0.704 Intron 1 2924 2953 ( 30 n); Pd: 0.000 Pa: 0.000 Exon 2 2954 3062 ( 109 n); score: 0.771 PGS (2804 2923,2954 3062) SGN-U324267- 3-phase translation of AGS-1 (+strand): . . . . . . 2804 AAAAATTACTGAAATAAACGTCTTATGATTCTCGTAGTTTTAATCCTCCCTTCTATTTCT K N Y - N K R L M I L V V L I L P S I S K I T E I N V L - F S - F - S S L L F L K L L K - T S Y D S R S F N P P F Y F . . . . . . : 2864 AAAAACCTCTAAGATCCCTTATTTCTTTAATATATTCGAGCTACATATTAATGTATTCAA : K N L - D P L F L - Y I R A T Y - C I Q : K T S K I P Y F F N I F E L H I N V F N : - K P L R S L I S L I Y S S Y I L M Y S : . . . . . . 2954 TATGCATTCTATTTATTTTATAATGTATTCGAGCTAGTTTTTAATGTATTCGAGCTACAT Y A F Y L F Y N V F E L V F N V F E L H M H S I Y F I M Y S S - F L M Y S S Y I I C I L F I L - C I R A S F - C I R A T . . . . . 3014 ATTATGTACTCGATTTGTGTTATAATGTATTCGAGATACTCTTTAATGT I M Y S I C V I M Y S R Y S L M L C T R F V L - C I R D T L - C Y Y V L D L C Y N V F E I L F N Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Mon Aug 28 21:40:55 2006 ________________________________________________________________________________ Sequence 2: C06HBa0016K14.1-2, from 1 to 5352, both strands analyzed. ... started at: Mon Aug 28 21:40:55 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 2 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 3 ******************************************************************************** EST sequence 5 +strand 762 n (File: SGN-U329579+) 1 TCAAGTGAAA GCACATGAGA TTCATCCGAA ATAAACTTCC GAAGCATAGA GACATGAAAA 61 ACAGGATGAA CTACAAGCTA CCTCTCCTAC TCGGCTCAAA ATCTCAAAAG GTCCAATGAA 121 TCTAGGGCTA AACTTGCCCT TTTTTCCAAA CCTCATCACA CCCTTCATGG GTGATACTCG 181 GAGCCAAACA TGATCACCCT CCATAAACAC TAAGGCTCTA ATTCCCTGAT CTGCATAACT 241 CTGTCGACTC TGAACTGTCA ATAATCTACT GAATCATACG GACTTGCTAC ATAGCATCTC 301 TAAGCAAGTT TGTATCCAAA GAGTCCATCT TCGCCGAATC AAACCAACCA ATCGGAGACC 361 TACACCGTCT TCCATACAAC GCCTCAAATG GGGCCATATG GATACTAGAG TGATAACTGT 421 TATTATAGCC AAACTCCGCT AAGGATAAAT GTTGATCCCA TCTAGCACCA AAATCGATCA 481 CACACGCTCG AAGCATATCT TCCAATACTT GCATGGTCCG CTCAGATTGA CCATCTGTTT 541 GAGGGTGAAA TGTTGTACTC ATATCTAACT GAGTACACAG ACCATGTTGT AATGCCTTCA 601 AGAATTGAGA TGTAAATAGT GAACCTCGAT CTGATATGAT AAAAATACGA ACTCCATGTA 661 TTCGAACAAT CTGACTGATA TATAGCTCGA CTAACTTTTC TGCCGTATAC TACATCCGAA 721 CCGGAATGAA GTGAGCAGAC TTGGTCAGCC TATCAACATA AC Predicted gene structure (within gDNA segment 2907 to 1): Exon 1 2208 2134 ( 75 n); cDNA 1 75 ( 75 n); score: 0.907 Intron 1 2133 2104 ( 30 n); Pd: 0.000 (s: 0.90), Pa: 0.000 (s: 0.89) ?? Exon 2 2103 1412 ( 692 n); cDNA 76 762 ( 687 n); score: 0.917 MATCH C06HBa0016K14.1-2- SGN-U329579+ 0.916 767 1.007 C PGS_C06HBa0016K14.1-2-_SGN-U329579+ (2208 2134,2103 1412) Alignment (genomic DNA sequence = upper lines): TCGAGTGAAA TCACATGAGA TTCATCCGGA ATATACTTCC GAAGCATAGA GACATGGAAA 2149 || ||||||| ||||||||| |||||||| | ||| |||||| |||||||||| |||||| ||| TCAAGTGAAA GCACATGAGA TTCATCCGAA ATAAACTTCC GAAGCATAGA GACATGAAAA 60 ACAGGATGAA CTGCCGACAA ACTAGGTGGC AAGGCCAACT TATAG-GCCA CCTCTCCCAC 2090 |||||||||| || | || | ||||||| || ACAGGATGAA CTACA..... .......... .......... .....AGCTA CCTCTCCTAC 90 TCGGCTCAAA ATCTCAAAAG GTCCAATGAA CCTAGGGCTA AGCTTGCCCT TCTTTCCAAA 2030 |||||||||| |||||||||| |||||||||| ||||||||| | |||||||| | |||||||| TCGGCTCAAA ATCTCAAAAG GTCCAATGAA TCTAGGGCTA AACTTGCCCT TTTTTCCAAA 150 CCTCATTACA CCCTTCATGG GTGATACTCG GAGCCAAACA TGATCACCCT CCATAAACAC 1970 |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCTCATCACA CCCTTCATGG GTGATACTCG GAGCCAAACA TGATCACCCT CCATAAACAC 210 CAAGGCTCTA ACTCTCCGAT CTGCATAACT CTTTTGTCGA CTCTGAGCTG TCAATAATCT 1910 ||||||||| | || | ||| |||||||||| | |||||| |||||| ||| ||||||||| TAAGGCTCTA ATTCCCTGAT CTGCATAACT C---TGTCGA CTCTGAACTG TCAATAATC- 266 ATACTGAATC ATACGAACTT GCTCCATAGC ATCTCTAAGC AAGTCTGTAT CCAAAGAGTC 1850 ||||||||| ||||| |||| ||| |||||| |||||||||| |||| ||||| |||||||||| -TACTGAATC ATACGGACTT GCTACATAGC ATCTCTAAGC AAGTTTGTAT CCAAAGAGTC 325 CATCTCCGCC GAATCAAACC AACCAATAGG AGATCTACAC CGTCTACCAT ATAATGCCTC 1790 ||||| |||| |||||||||| ||||||| || ||| |||||| ||||| |||| | || ||||| CATCTTCGCC GAATCAAACC AACCAATCGG AGACCTACAC CGTCTTCCAT ACAACGCCTC 385 AAATGGGGCC ATTTGGATAC TAGAGTGATA ACTGTTATTA TAGGCAAACT CCGCTAAGGG 1730 |||||||||| || ||||||| |||||||||| |||||||||| ||| |||||| ||||||||| AAATGGGGCC ATATGGATAC TAGAGTGATA ACTGTTATTA TAGCCAAACT CCGCTAAGGA 445 TAAATGTCGA TCCCATCTTG CACCAAAATC GATCACACAC GCTCGAAGCA TATCTTCCAA 1670 ||||||| || |||||||| | |||||||||| |||||||||| |||||||||| |||||||||| TAAATGTTGA TCCCATCTAG CACCAAAATC GATCACACAC GCTCGAAGCA TATCTTCCAA 505 TACCTGAATG GTCCGCTCAG ATTGACCATC TGTCTGAGGG TGAAATGCCG TACTCATATC 1610 ||| || ||| |||||||||| |||||||||| ||| |||||| ||||||| | |||||||||| TACTTGCATG GTCCGCTCAG ATTGACCATC TGTTTGAGGG TGAAATGTTG TACTCATATC 565 TAACTGAGTA CCCAGACCAT GTTGTAATGC CTTCCAGAAG TGAGAAGTAA ATAGTGAACC 1550 |||||||||| | |||||||| |||||||||| |||| |||| ||||| |||| |||||||||| TAACTGAGTA CACAGACCAT GTTGTAATGC CTTCAAGAAT TGAGATGTAA ATAGTGAACC 625 TCGATCTGAT ATGATAGAAA GAGGAACTCC ATGTAGTCGT ACAATCTGAC TGATATATAG 1490 |||||||||| |||||| ||| | ||||||| ||||| ||| |||||||||| |||||||||| TCGATCTGAT ATGATAAAAA TACGAACTCC ATGTATTCGA ACAATCTGAC TGATATATAG 685 TTCGGCTAAC TTTTCTGCTG TATACTTTAC CCGAACCGGG ATGAAGTGGG CAGACTTGGT 1430 ||| ||||| |||||||| | |||||| | ||||||||| |||||||| | |||||||||| CTCGACTAAC TTTTCTGCCG TATACTACAT CCGAACCGGA ATGAAGTGAG CAGACTTGGT 745 CAGTCTATCA ACAACAAC 1412 ||| |||||| || | ||| CAGCCTATCA AC-ATAAC 762 hqPGS_C06HBa0016K14.1-2-_SGN-U329579+ (2208 2134,2103 1412) ******************************************************************************** EST sequence 1 -strand 897 n (File: SGN-U342484-) 1 AATTTTAACG AGGTCCCCAG ATTATGTGGT AAAGCTTTCC AGAAATAAGA AGTAAATAGT 61 GACCCTCGAT CTGATATGAT AGAAATGGAA ACTCCAGGTA GTGGACCAAT GTGCTGAAAT 121 ATAGCTCAGC TACTTTTTCN ACCGTATACT TCACCGAACC CGGAATGAAG TGGGCAGATT 181 TGGTCAGCCT ATCATCACAA ACCCAAATAG AGTCATAACC ACCCACTGTG GTAGGCAAAC 241 CCACAACAAA GTCCATAGTA ATCCACTCCC ACGTCCAAGT AAGAATAGGC ATCCTTTGAG 301 ATACACCCCC AGGCCACTGG TGCTCACACT TGACCAACTG GCAAGTCAAA CACCTCAAAA 361 CAAAGTCTGT AATATCTCTC TTCATCCCAC ACCACCAGTA ATGCTGACTC AGATCATGAT 421 ACATTTTCGC CTCTCCCGGA TGCATAGAAT ATCGAGAACA ATGGGCCTCC TCAAGGATCA 481 ATCTAATCAA ATCGCCTGTC TTGGGCACAC AAATCCTGCC TCCGATCCTC AAGACACCAT 541 CAGAATCAAG GACAACCTCC TTAGCTTCCC CTCTCAATAC TTTGTCTCGA ATGAGACATA 601 ATTTTTCGTC ATCAAACTGG TGTGCACGAA TCTGCTTGAC TAAAGAAGAT CGAGCCTCAA 661 TAAAAGCAAT CATCCCATCA CTCTCCTCTG AGATCTGTAA TCGGACAAGA CTGTTAGCTA 721 ACAATTGAAC ATCTCTAGCC AATGGTCTTT CCTCAACACT AAGAGATGCA AGACTCCCCA 781 TGCTATGAGT CTTCCTACTC AAAGCATCGG CCACAACATT GGCCTTCCCC GGATGATACA 841 AAATCCTCGT GCCGAATTTC CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCGG Predicted gene structure (within gDNA segment 4014 to 1): Exon 1 1609 770 ( 840 n); cDNA 6 844 ( 839 n); score: 0.894 MATCH C06HBa0016K14.1-2- SGN-U342484- 0.894 840 0.936 C PGS_C06HBa0016K14.1-2-_SGN-U342484- (1609 770) Alignment (genomic DNA sequence = upper lines): TAACTGAGTA CCCAGACCAT GTTGTAATGC CTTCCAGAAG TGAGAAGTAA ATAGTGAACC 1550 |||| || |||||| || || |||| || |||||||| | |||||||| ||||||| || TAACGAGGTC CCCAGATTAT GTGGTAAAGC TTTCCAGAAA TAAGAAGTAA ATAGTGACCC 65 TCGATCTGAT ATGATAGAAA GAGGAACTCC ATGTAGTCGT ACAATCTGAC TGATATATAG 1490 |||||||||| |||||||||| | |||||| | ||||| | |||| || | ||| |||||| TCGATCTGAT ATGATAGAAA TGGAAACTCC AGGTAGTGGA CCAATGTG-C TGAAATATAG 124 TTCGGCTAAC TTTTCTGCTG TATACTTTAC CCGAACCGGG ATGAAGTGGG CAGACTTGGT 1430 || |||| ||||| | | ||||||| || | | |||| |||||||||| |||| ||||| CTCAGCTACT TTTTCNACCG TATACTTCAC CGAACCCGGA ATGAAGTGGG CAGATTTGGT 184 CAGTCTATCA ACAACAACCC AAATAGAGTC ATAACCACCC ACTGTGGTAG GCAAACCCAC 1370 ||| |||||| || ||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGCCTATCA TCACAAACCC AAATAGAGTC ATAACCACCC ACTGTGGTAG GCAAACCCAC 244 AACAAAGTCC ATAGTAATCC GCTCCCACTT CCAAGTAGGA ATAGGCATCC TCTGAGATAC 1310 |||||||||| |||||||||| ||||||| | ||||||| || |||||||||| | |||||||| AACAAAGTCC ATAGTAATCC ACTCCCACGT CCAAGTAAGA ATAGGCATCC TTTGAGATAC 304 ACCCCCTGGC CGCTGGTGCT CACATTTGAC CTGCTGGCAA GTCAAACACC TAGAAACAAA 1250 |||||| ||| | |||||||| |||| ||||| | ||||||| |||||||||| | ||||||| ACCCCCAGGC CACTGGTGCT CACACTTGAC CAACTGGCAA GTCAAACACC TCAAAACAAA 364 GTCTGAAATA TCTCTCTTCA TCCCACACCA CCAGTAATGC TGACTCAGAT CATGATACAT 1190 ||||| |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTGTAATA TCTCTCTTCA TCCCACACCA CCAGTAATGC TGACTCAGAT CATGATACAT 424 CTTTGCCGCT CCCGGATGGA TGGAATACCG AGTACAATGG GCCTCCTCAA GAATCAATCT 1130 || ||| || |||||||| | | ||||| || || ||||||| |||||||||| | |||||||| TTTCGCCTCT CCCGGATGCA TAGAATATCG AGAACAATGG GCCTCCTCAA GGATCAATCT 484 AATCAAATCG CCTGTCCTGG GCACACAAAT TCTGCCTCCG ATCCGCAAGA CACCATCAGA 1070 |||||||||| |||||| ||| |||||||||| ||||||||| |||| ||||| |||||||||| AATCAAATCG CCTGTCTTGG GCACACAAAT CCTGCCTCCG ATCCTCAAGA CACCATCAGA 544 ATCAAGGACA GCCTCCTTAG CTTCCCCTCT CAATACTCTG TCTCGAATGA GACATAATTT 1010 |||||||||| ||||||||| |||||||||| ||||||| || |||||||||| |||||||||| ATCAAGGACA ACCTCCTTAG CTTCCCCTCT CAATACTTTG TCTCGAATGA GACATAATTT 604 TTCGTCATCA AATTGGTGTG CACGAATTTG CTCGACTAAA GAAGACCGAG CCTCAATAAA 950 |||||||||| || ||||||| ||||||| || || ||||||| ||||| |||| |||||||||| TTCGTCATCA AACTGGTGTG CACGAATCTG CTTGACTAAA GAAGATCGAG CCTCAATAAA 664 AGCAATCATC CCCTCACTCT CTTCTGAAAT CTGCAAGCGG ACAAGACTGT TAGCTAATAT 890 |||||||||| || ||||||| | ||||| || ||| || ||| |||||||||| ||||||| | AGCAATCATC CCATCACTCT CCTCTGAGAT CTGTAATCGG ACAAGACTGT TAGCTAACAA 724 CTGCACATCT CTAGCCAATG GTCTCTCCAT AATACTAAGT GCTGCAAGAC TCCCCATGCT 830 || |||||| |||||||||| |||| ||| || |||||| | |||||||| |||||||||| TTGAACATCT CTAGCCAATG GTCTTTCCTC AACACTAAGA GATGCAAGAC TCCCCATGCT 784 AGGAGTCTTC CTACTCAGAG CATCGGCCAT AACATTGGCC TTTCCTGGAT GATACAGAAT 770 | |||||||| ||||||| || ||||||||| |||||||||| || || |||| |||||| ||| ATGAGTCTTC CTACTCAAAG CATCGGCCAC AACATTGGCC TTCCCCGGAT GATACAAAAT 844 hqPGS_C06HBa0016K14.1-2-_SGN-U342484- (1609 770) ******************************************************************************** EST sequence 4 +strand 854 n (File: SGN-U339313+) 1 ATTGAAACCA ATGCTGGAGC TCCACCGCGG TGGCGGTCGC TCTAGAACTA GTGGATCCCC 61 CGGGCTGCAG GAATTCGGCA CGAGGACAAA TTACATATCC TCAACAACAA CACTTCAACA 121 AATTACATAT CCTCAACAAG AATAGTTCAA CAAATTATAT ATCCGCCATA ACAAGCTCAA 181 TATTAATCAA TCACAAGTTC TGCAGAAAGG CAATAAGAAG ATCGAGAAAA TACGATATCA 241 AGTTCACTAA TGATAAGTGT GTAATGCAAT GGAATGCAAT GTCAAGTATA ATGATGCATG 301 TCTGACCTAG TGATATACAC CCGCTGTCTC TCAGTCCGGG ACCCTTGGGG GACATATCTG 361 TCCATGCATC TGTCGCGGCG CACGACACGA TCCTCGATAA TAGTAACCAT CACGGCGCGC 421 GATACGTTCC TCGAAATATA GTATCCATCG CGGCGCGCGA TACGTCCCTC GAAATGGTAC 481 ATCCTCTTAA GTACCCATTA TTTCTCAATG CACATAACAC TTGCCACAAC CAATGCCTCA 541 GAATAATGCA GATGACAAGT TCACACATAT AATGAGGAGA TGACCATTTT CATAACATCC 601 TACAGTTCAC AACCACACAA ACATCAAGTT ACATCAACAA TGATTCAACA AGCCTTCGAT 661 TCTTTCTCAA CACAACACAC ATAAACCATT AATCACATTG CCTTTTGTAT CCCCTTTCTT 721 TCATCATTAG AANTAATCAA TGTGGACAAG TCAACCCATA CCCCATCCCG TACTCCAACA 781 TATAACACAA GGACATACAC GCATTCATAC ACTTAGAAGA ATTAGAAAAT CCCTTGCCTC 841 AATAGTCCAC AAGG Predicted gene structure (within gDNA segment 5352 to 1): Exon 1 4024 4013 ( 12 n); cDNA 117 128 ( 12 n); score: 0.750 Intron 1 4012 3956 ( 57 n); Pd: 0.711 (s: 0), Pa: 0.000 (s: 0.58) Exon 2 3955 3565 ( 391 n); cDNA 129 515 ( 387 n); score: 0.777 Intron 2 3564 199 (3366 n); Pd: 0.000 (s: 0.60), Pa: 0.000 (s: 0) Exon 3 198 172 ( 27 n); cDNA 516 543 ( 28 n); score: 0.685 MATCH C06HBa0016K14.1-2- SGN-U339313+ 0.777 430 0.504 C PGS_C06HBa0016K14.1-2-_SGN-U339313+ (4024 4013,3955 3565,198 172) Alignment (genomic DNA sequence = upper lines): AACAAAATAC GGGTACTCCT TACACCCTAT CTAAACCTCC ACGCTACAGC CTAACAGTTT 3965 |||||| ||| AACAAATTAC AT........ .......... .......... .......... .......... 128 ACCAAACACA CCACTCAATA ACCACACTCT ATCAGTTTAC ACATTCTCAA TATCAA-CTC 3906 | | ||||| | | | | | | || ||| | || | | | || ||| ||| .........A TC-CTCAACA AGAATAGTTC AACAAATTAT ATATCCGCCA TAACAAGCTC 178 AATATTCAAC AATCACAGAC TTC-ACAGAG AAACAATCAC AAGATCAGCA AAACACATGT 3847 |||||| | | ||||||| | ||| |||| | |||| | |||||| | ||| || AATATTAATC AATCACA-AG TTCTGCAGAA AGGCAATAAG AAGATCGAGA AAATACGATA 237 TCAAGTTCAT CAATAATAAG ATGTGCAATG TCATGAGATG CAATGTCAAA TATCGTGATG 3787 ||||||||| ||| ||||| |||| |||| ||| ||| ||||||||| ||| ||||| TCAAGTTCAC TAATGATAAG -TGTGTAATG CAATGGAATG CAATGTCAAG TATAATGATG 296 CATGTCTTTC CTAACGATAC ACACCCGCTG TCTCTCAGTT CGGGACCCAT GGGGGACATA 3727 ||||||| | ||| |||| |||||||||| ||||||||| |||||||| | |||||||||| CATGTCTGAC CTAGTGATAT ACACCCGCTG TCTCTCAGTC CGGGACCCTT GGGGGACATA 356 TCTGTCCATG CATCTGTCGC GGCGCACGAC ACGACCCTCG ATAAGTAGTA ACCTTCGCGG 3667 |||||||||| |||||||||| |||||||||| |||| ||||| |||| ||||| ||| || ||| TCTGTCCATG CATCTGTCGC GGCGCACGAC ACGATCCTCG ATAA-TAGTA ACCATCACGG 415 CGCGTGATAC GTCCCTCGAA ATATAATATC CATCGCGGCG CGCGATACGA CCCTCGAAAA 3607 |||| ||||| || ||||||| ||||| |||| |||||||||| ||||||||| |||||| ||| CGCGCGATAC GTTCCTCGAA ATATAGTATC CATCGCGGCG CGCGATACGT CCCTCG-AAA 474 TAGTACACCA TCCT-CTTAC CACAACAATG TCTCAGATAC A-ATGCAAAT GACATGCTCA 3549 | ||||| | || | ||| | | | || ||||| || | | || TGGTACATCC TCTTAAGTAC C-C-ATTATT TCTCA-ATGC ACAT...... .......... 515 AATGAAAAGG GAGGAGATAA CCATTTGATA ACCAAACACA ATTTACAACA AGAAATACAA 3489 .......... .......... .......... .......... .......... .......... 515 CACCAACAAA TAAACAACAA GTCTCCAAAT CATTCTCAAC ATCACACAAG GAAATACACG 3429 .......... .......... .......... .......... .......... .......... 515 AATTTCGTAC GCTTAACAAG AGTTTAGAAA TCCACTTGCC TTAGTCACTT GAATAATCAC 3369 .......... .......... .......... .......... .......... .......... 515 TACGGGACTT GAGCCTTCCC TTTAAGTTGA GCTTCCAAAT CGATGCAATC TATTCAAATA 3309 .......... .......... .......... .......... .......... .......... 515 TGTATATTCA CAATAAGGTT TGGAACTAAC AACACCCACA TTATTATGTG TTTAACCTTG 3249 .......... .......... .......... .......... .......... .......... 515 ACATAAAACT TCACCCAAAT TTATAATCAA GTTTGTAATT CTTGATGTCA ATTAACATGT 3189 .......... .......... .......... .......... .......... .......... 515 GAACTCTCAT GTTTTCCAAA TTTAAGCTTA CGGTTAAATT TCAATTTCTC CAAATATCAT 3129 .......... .......... .......... .......... .......... .......... 515 AACTTTAACG ATATCTATAT AATATAATAT ATCTAATATT TAATTACCTA TCTCAATATA 3069 .......... .......... .......... .......... .......... .......... 515 TCACACTTGA ATCTTAATAT GATAATAATA AGAAAATTCT AAGAAACAGT GAAGCCTTAA 3009 .......... .......... .......... .......... .......... .......... 515 GTTACAGAGA AAATCACGAG GACAAAAAAA TATATTTTAA ATCCTCAAAT GGATACCCTA 2949 .......... .......... .......... .......... .......... .......... 515 TATTCCTATC TATTATTTTT CAATCATCTT GATGCAATTA TATTCCCAAC AATTATCACA 2889 .......... .......... .......... .......... .......... .......... 515 TGATTGGCAT GACCCTCTTT CTAAATTTCA AAATTACTTC ATACCAATAT ACCAACTTCC 2829 .......... .......... .......... .......... .......... .......... 515 AAATATTATT TCTTTATTCC ATATTCATTT TGCCATGTTT CACATTCCAA AGCACATATT 2769 .......... .......... .......... .......... .......... .......... 515 AAAGTTAACA TAAAAATTAT AATTGTACAT TAATTACAAG ACATATTCAG CAGCCTCAAT 2709 .......... .......... .......... .......... .......... .......... 515 GCAAAGTTTA AAATTATTAC CTGAATCAAG TGCACTTGTT CGACGGCGCT GCGAGTGTTT 2649 .......... .......... .......... .......... .......... .......... 515 TTCAGACTTT CTCCTCTTCT TTGTTTTTCG TCACACGTTT AGGTTAGTTT TGTTCCACAT 2589 .......... .......... .......... .......... .......... .......... 515 ATTTAATTAA ATAAAGGCTA AATAGTATAG GGTCTTAAAT AAATTATCAT TTTAGCCCAT 2529 .......... .......... .......... .......... .......... .......... 515 AAACTATCGA TTAATTAACT CAAGTTACAC TAATATTTAA AATTTCCAAA AATGACCTTC 2469 .......... .......... .......... .......... .......... .......... 515 CGGGTCATTA CATTATCCAC CACTAAAAAT CATGTTCGTC CTCGAACATA AAGTAAAAGA 2409 .......... .......... .......... .......... .......... .......... 515 AAGTACCTGA TGCTTCAAAA AGTTGAGGAT ATCTGGCACG CATGTCAGAC TCTGTCTCCC 2349 .......... .......... .......... .......... .......... .......... 515 AAGTTGCCTC TCTCACTGAT CGGTGCTTCC ATTGCACCTT CACTGAAGCA ATCTCTTTGG 2289 .......... .......... .......... .......... .......... .......... 515 TCCTAAGCTT TCGAATTTGC CTATCCAAAA TAGCTATAGG CTCCTCCTCA AATGTCAAGT 2229 .......... .......... .......... .......... .......... .......... 515 CTGGACCCAG CTCCACAGAA TCGAGTGAAA TCACATGAGA TTCATCCGGA ATATACTTCC 2169 .......... .......... .......... .......... .......... .......... 515 GAAGCATAGA GACATGGAAA ACAGGATGAA CTGCCGACAA ACTAGGTGGC AAGGCCAACT 2109 .......... .......... .......... .......... .......... .......... 515 TATAGGCCAC CTCTCCCACT CGGCTCAAAA TCTCAAAAGG TCCAATGAAC CTAGGGCTAA 2049 .......... .......... .......... .......... .......... .......... 515 GCTTGCCCTT CTTTCCAAAC CTCATTACAC CCTTCATGGG TGATACTCGG AGCCAAACAT 1989 .......... .......... .......... .......... .......... .......... 515 GATCACCCTC CATAAACACC AAGGCTCTAA CTCTCCGATC TGCATAACTC TTTTGTCGAC 1929 .......... .......... .......... .......... .......... .......... 515 TCTGAGCTGT CAATAATCTA TACTGAATCA TACGAACTTG CTCCATAGCA TCTCTAAGCA 1869 .......... .......... .......... .......... .......... .......... 515 AGTCTGTATC CAAAGAGTCC ATCTCCGCCG AATCAAACCA ACCAATAGGA GATCTACACC 1809 .......... .......... .......... .......... .......... .......... 515 GTCTACCATA TAATGCCTCA AATGGGGCCA TTTGGATACT AGAGTGATAA CTGTTATTAT 1749 .......... .......... .......... .......... .......... .......... 515 AGGCAAACTC CGCTAAGGGT AAATGTCGAT CCCATCTTGC ACCAAAATCG ATCACACACG 1689 .......... .......... .......... .......... .......... .......... 515 CTCGAAGCAT ATCTTCCAAT ACCTGAATGG TCCGCTCAGA TTGACCATCT GTCTGAGGGT 1629 .......... .......... .......... .......... .......... .......... 515 GAAATGCCGT ACTCATATCT AACTGAGTAC CCAGACCATG TTGTAATGCC TTCCAGAAGT 1569 .......... .......... .......... .......... .......... .......... 515 GAGAAGTAAA TAGTGAACCT CGATCTGATA TGATAGAAAG AGGAACTCCA TGTAGTCGTA 1509 .......... .......... .......... .......... .......... .......... 515 CAATCTGACT GATATATAGT TCGGCTAACT TTTCTGCTGT ATACTTTACC CGAACCGGGA 1449 .......... .......... .......... .......... .......... .......... 515 TGAAGTGGGC AGACTTGGTC AGTCTATCAA CAACAACCCA AATAGAGTCA TAACCACCCA 1389 .......... .......... .......... .......... .......... .......... 515 CTGTGGTAGG CAAACCCACA ACAAAGTCCA TAGTAATCCG CTCCCACTTC CAAGTAGGAA 1329 .......... .......... .......... .......... .......... .......... 515 TAGGCATCCT CTGAGATACA CCCCCTGGCC GCTGGTGCTC ACATTTGACC TGCTGGCAAG 1269 .......... .......... .......... .......... .......... .......... 515 TCAAACACCT AGAAACAAAG TCTGAAATAT CTCTCTTCAT CCCACACCAC CAGTAATGCT 1209 .......... .......... .......... .......... .......... .......... 515 GACTCAGATC ATGATACATC TTTGCCGCTC CCGGATGGAT GGAATACCGA GTACAATGGG 1149 .......... .......... .......... .......... .......... .......... 515 CCTCCTCAAG AATCAATCTA ATCAAATCGC CTGTCCTGGG CACACAAATT CTGCCTCCGA 1089 .......... .......... .......... .......... .......... .......... 515 TCCGCAAGAC ACCATCAGAA TCAAGGACAG CCTCCTTAGC TTCCCCTCTC AATACTCTGT 1029 .......... .......... .......... .......... .......... .......... 515 CTCGAATGAG ACATAATTTT TCGTCATCAA ATTGGTGTGC ACGAATTTGC TCGACTAAAG 969 .......... .......... .......... .......... .......... .......... 515 AAGACCGAGC CTCAATAAAA GCAATCATCC CCTCACTCTC TTCTGAAATC TGCAAGCGGA 909 .......... .......... .......... .......... .......... .......... 515 CAAGACTGTT AGCTAATATC TGCACATCTC TAGCCAATGG TCTCTCCATA ATACTAAGTG 849 .......... .......... .......... .......... .......... .......... 515 CTGCAAGACT CCCCATGCTA GGAGTCTTCC TACTCAGAGC ATCGGCCATA ACATTGGCCT 789 .......... .......... .......... .......... .......... .......... 515 TTCCTGGATG ATACAGAATG GTCACATCAT AGTCCTTCAA CAACTCAAGC CATCTCCGTT 729 .......... .......... .......... .......... .......... .......... 515 GCCTCAAGTT CAAATCCCTC TAGCTAAAGA TATATTGAAG ACTCCGATGA TCAGTGAAGA 669 .......... .......... .......... .......... .......... .......... 515 TCTCACAATG CACCCCATAC AAATAATGAC GCCATAACTT AAGTACAAAT ACCACAGCCG 609 .......... .......... .......... .......... .......... .......... 515 CCAACTCCAA ATCATGAGTA GGGTAGTTTT TCTCATGGGA TTTCAATTGC CTAGAAGCAT 549 .......... .......... .......... .......... .......... .......... 515 AAGCAATCAC TTTCCCCTTC TGCATCAACA CACCACCCAA ACCAACTCCT GAAGCATCAC 489 .......... .......... .......... .......... .......... .......... 515 AATACACAGT AAAGTCTACA CCTTCCTCAG GTAGAGTCAA CACAGGAGCA GAAGTCAACA 429 .......... .......... .......... .......... .......... .......... 515 AAGTCTTGAG TTTTTGAAAG CTCTCCTCAC ACTCATCAGA CCACTGAAAA CTCACATCCT 369 .......... .......... .......... .......... .......... .......... 515 GTCGAGTCAA TCTAGTCAAT GGAGCTGCAA TAGTAGAGAA ACTCTGAACA AATCGTCGAT 309 .......... .......... .......... .......... .......... .......... 515 AATAGCCTGC CAATCCCACA AAACTCCTAA TCTCAGTAGG TGAGGTAGGT CGCGTCCAGC 249 .......... .......... .......... .......... .......... .......... 515 CTCTAACTGC CTTAATTTTG GCTGGATCTA CTCTAATACC CTCCTTGGAC ACCACGTGCC 189 | ||| |||| .......... .......... .......... .......... .......... AACACTTGCC 525 CCAA-GAATG TCACAGAA 172 ||| |||| | ||||| ACAACCAATG CCTCAGAA 543 hqPGS_C06HBa0016K14.1-2-_SGN-U339313+ (4024 4013,3955 3565) ******************************************************************************** EST sequence 2 -strand 681 n (File: SGN-U328762-) 1 TTCAACAAAT TATATATCCT CAATAACAAG CTCAGTATTC ATCAATCACA AATTTCGCAG 61 AAATTCAATC ACAAGATCAG CAAAACACAA TATCAAGTTC ACCAATGATA AGTATGTGTA 121 ATGCATTGTC AAGTATAATG ATGCATGTCT GACCTAGTGA TACACACCCG CTGTCTCTCA 181 GTCTGGGAAC CATGGGGGAC ATATCTGTCC ATGCATCTGT CGCGGCGCAC GACACGACCC 241 TCGATAATAG TAACCATCGC GGCGCGCGAT ACGTCCCTCA AAATATAGTA TCCATCGCGG 301 CATGCGATAC GTCCCTCGAA ATATAGTATC CATCGCGGCG CGCGATACGT CCCTCGAAAT 361 TGTACATCCT CTTAAGTACT CATTCTTTCT CAATGCACAT AACACTTGTC ACAACCAATG 421 CCTCATAATA ATGCAGATGG CAAGTTCACA CAAATAATGA GGAGATGACC ATTTTCATAA 481 CATTGCACAA TTCAAAACCA CACAAACATG AAATCACATC AACAATGATT CAACAATCCT 541 TCATCCCTTT CTGAACACAT CACACTTAAA TATTTAATCA CATTGCCTTT TTTTTTATCC 601 TTTTTTTTTC ATCATTAAAT TAATCAATGT GGACAAGTCA ATCCATCACC AATCCCGTTA 661 CTCCCAAACA TATACCACAA G Predicted gene structure (within gDNA segment 5352 to 1): Exon 1 3932 3605 ( 328 n); cDNA 6 323 ( 318 n); score: 0.843 MATCH C06HBa0016K14.1-2- SGN-U328762- 0.843 328 0.482 C PGS_C06HBa0016K14.1-2-_SGN-U328762- (3932 3605) Alignment (genomic DNA sequence = upper lines): CAGTTTACAC ATTCTCAATA TCAA-CTCAA TATTCAACAA TCACAGACTT CACAGAGAAA 3874 || ||| | || ||||||| ||| |||| |||||| ||| ||||| | || | |||| | CAAATTATAT ATCCTCAATA ACAAGCTCAG TATTCATCAA TCACAAATTT CGCAGAAATT 65 CAATCACAAG ATCAGCAAAA CACATGTTCA AGTTCATCAA TAATAAGATG TGCAATGTCA 3814 |||||||||| |||||||||| |||| ||| |||||| ||| | || | | | | |||| CAATCACAAG ATCAGCAAAA CACAATATCA AGTTCACCAA TGAT-A-A-G T---ATGT-G 118 TGAGATGCAA TGTCAAATAT CGTGATGCAT GTCTTTCCTA ACGATACACA CCCGCTGTCT 3754 | | ||||| |||||| ||| |||||||| |||| |||| |||||||| |||||||||| T-A-ATGCAT TGTCAAGTAT AATGATGCAT GTCTGACCTA GTGATACACA CCCGCTGTCT 176 CTCAGTTCGG GACCCATGGG GGACATATCT GTCCATGCAT CTGTCGCGGC GCACGACACG 3694 |||||| || || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAGTCTGG GAACCATGGG GGACATATCT GTCCATGCAT CTGTCGCGGC GCACGACACG 236 ACCCTCGATA AGTAGTAACC TTCGCGGCGC GTGATACGTC CCTCGAAATA TAATATCCAT 3634 |||||||||| | |||||||| ||||||||| | |||||||| |||| ||||| || ||||||| ACCCTCGATA A-TAGTAACC ATCGCGGCGC GCGATACGTC CCTCAAAATA TAGTATCCAT 295 CGCGGCGCGC GATACGACCC TCGAAAATA 3605 |||||| || |||||| ||| ||| ||||| CGCGGCATGC GATACGTCCC TCG-AAATA 323 hqPGS_C06HBa0016K14.1-2-_SGN-U328762- (3932 3605) ******************************************************************************** EST sequence 3 +strand 904 n (File: SGN-U337987+) 1 GGGACCGGGC CCCCCCTCGA GTTTTTTTTT TTTTTTTTTG CAGAAATAAA ATATAAGTAA 61 CTTAATTAAA CCCCCAAGAC CTGGGTAGTC ACGTGTACAA GCCTCTAAAG TATTACAAGA 121 TTCAAAAGAA AAAAACTCAA GTCTCAAATG AACTTGTTTC TAGAATAGAA CAAGATCATA 181 ATTATGGAGA AGAAAGTCTG CTGAGATGGA AACAGCTACC TCACAAATCT CCATGAAGCC 241 TCGGAAAAGA AGAGACTGAC AAGTACAACA AAAATCCAGG CTCATAACCT ACAAAAATTT 301 GTAGAAGCAA GGGGTGAGTA CCAAACCACA CGGTACTCAG CAAGTAAACC TCTAAACATA 361 AGCTAAGGGG ATGAAATACG GGTACTCCTA CCACCCCCAA CCGAACCTCC ACAAATACAA 421 CCTGCATTAA AATCAACCCA ACCTAACAGC TCACAATTTA CATAGCACAT AGCTCAACAA 481 CAACACTTAG GCAAATTACA TATCCTCAAC AACAACACTT CAACAAATTA CATATCCTCA 541 ACAACAATAC TTCAACAAAT TACATATCCT CAACAACAAT ACTTCAACAA ATTACATATC 601 CGCAATAACA AGCTCAATAT TGATCAATCA CAAGTTCCGC AGAAAAGCAA TCAAAACATC 661 AGCGAAATAC GATATCAAGT TCACTAATGG TAAGTATGTG CAATGCAATG GAATGCAATG 721 TCAAGTATAA TGATGCATGT CTGACCTAGT GATACACACC CACTGTCTCT CAGTCCGGGA 781 CCCATGGGAG ACATATATGT CCATGCATCT GTCGCGGCGC ACGACACGAC CCTCGATTAT 841 AGTAACCATC GCAGCGCGCG GAACGTCCCT CGAATATAGT ATCCATCGCC GCGCGCGATA 901 TGTN Predicted gene structure (within gDNA segment 5352 to 2405): Exon 1 4311 3973 ( 339 n); cDNA 85 423 ( 339 n); score: 0.758 PPA cDNA 39 21 MATCH C06HBa0016K14.1-2- SGN-U337987+ 0.758 339 0.375 C PGS_C06HBa0016K14.1-2-_SGN-U337987+ (4311 3973) Alignment (genomic DNA sequence = upper lines): GTTGTCACGT GCACAAGCCT CTAATGTA-A AC-A-TTAGA ATTGAAACAA AATAGAAGTC 4255 || ||||||| | |||||||| |||| ||| || | | | | |||| || || ||||| GTAGTCACGT GTACAAGCCT CTAAAGTATT ACAAGATTCA AAAGAAA-AA AACTCAAGTC 143 TAAAATGAAG TTGTCTTTCA AGTAAAAACA AAATCATAA- ACTGGAGTAG GAAAGTTCGC 4196 | ||||||| |||| | | | | | |||| | ||||||| ||||| | |||||| || TCAAATGAAC TTGTTTCTAG AAT-AGAACA AGATCATAAT TATGGAG-AA GAAAGTCTGC 201 TGAGATGACA AACAGCTACC TCACAATCCT CCACAAGATG CCTCGAAAAC GAAGAGAATA 4136 ||||||| | |||||||||| |||||| || | || || | ||||| ||| ||||||| | TGAGATG-GA AACAGCTACC TCACAAATCT -C-CATGAAG CCTCGGAAAA GAAGAGACTG 258 GAAGGTATCA CAAAGATCCA GGCTCGTAAC CTACAAAAAT TTGTAGAAGC AAGGGGTGAG 4076 | ||| | |||| ||||| ||||| |||| |||||||||| |||||||||| |||||||||| ACAAGTACAA CAAAAATCCA GGCTCATAAC CTACAAAAAT TTGTAGAAGC AAGGGGTGAG 318 TACCAAACCA CGCGGTACCC AACAAGCAAA CCTCTAAACA CAAGTTAAGT GAACAAAATA 4016 |||||||||| | |||||| | | |||| ||| |||||||||| ||| |||| | | ||||| TACCAAACCA CACGGTACTC AGCAAGTAAA CCTCTAAACA TAAGCTAAGG GGATGAAATA 378 CGGGTACTCC TTACA-CCCT ATCTAAACCT CCAC-GCTAC AGCCT 3973 |||||||||| | || ||| | | ||||| |||| ||| | ||| CGGGTACTCC TACCACCCCC AACCGAACCT CCACAAATAC AACCT 423 hqPGS_C06HBa0016K14.1-2-_SGN-U337987+ (4311 3973) Total number of EST alignments reported: 5 ________________________________________________________________________________ Predicted gene locations (2) in segment 1 to 5352: PGL 1 (- strand): 2208 770 AGS-1 (2208 2134,2103 770) SCR (e 0.907 d 0.000 a 0.000,e 0.917) Exon 1 2208 2134 ( 75 n); score: 0.907 Intron 1 2133 2104 ( 30 n); Pd: 0.000 Pa: 0.000 Exon 2 2103 770 (1334 n); score: 0.917 PGS (1609 770) SGN-U342484- PGS (2208 2134,2103 1412) SGN-U329579+ 3-phase translation of AGS-1 (-strand): . . . . . . 2208 TCGAGTGAAATCACATGAGATTCATCCGGAATATACTTCCGAAGCATAGAGACATGGAAA S S E I T - D S S G I Y F R S I E T W K R V K S H E I H P E Y T S E A - R H G K E - N H M R F I R N I L P K H R D M E . . : . . . . 2148 ACAGGATGAACTGCC : GCCACCTCTCCCACTCGGCTCAAAATCTCAAAAGGTCCAATGAAC T G - T A : A T S P T R L K I S K G P M N Q D E L P : P P L P L G S K S Q K V Q - T N R M N C : R H L S H S A Q N L K R S N E . . . . . . 2058 CTAGGGCTAAGCTTGCCCTTCTTTCCAAACCTCATTACACCCTTCATGGGTGATACTCGG L G L S L P F F P N L I T P F M G D T R - G - A C P S F Q T S L H P S W V I L G P R A K L A L L S K P H Y T L H G - Y S . . . . . . 1998 AGCCAAACATGATCACCCTCCATAAACACCAAGGCTCTAACTCTCCGATCTGCATAACTC S Q T - S P S I N T K A L T L R S A - L A K H D H P P - T P R L - L S D L H N S E P N M I T L H K H Q G S N S P I C I T . . . . . . 1938 TTTTGTCGACTCTGAGCTGTCAATAATCTATACTGAATCATACGAACTTGCTCCATAGCA F C R L - A V N N L Y - I I R T C S I A F V D S E L S I I Y T E S Y E L A P - H L L S T L S C Q - S I L N H T N L L H S . . . . . . 1878 TCTCTAAGCAAGTCTGTATCCAAAGAGTCCATCTCCGCCGAATCAAACCAACCAATAGGA S L S K S V S K E S I S A E S N Q P I G L - A S L Y P K S P S P P N Q T N Q - E I S K Q V C I Q R V H L R R I K P T N R . . . . . . 1818 GATCTACACCGTCTACCATATAATGCCTCAAATGGGGCCATTTGGATACTAGAGTGATAA D L H R L P Y N A S N G A I W I L E - - I Y T V Y H I M P Q M G P F G Y - S D N R S T P S T I - C L K W G H L D T R V I . . . . . . 1758 CTGTTATTATAGGCAAACTCCGCTAAGGGTAAATGTCGATCCCATCTTGCACCAAAATCG L L L - A N S A K G K C R S H L A P K S C Y Y R Q T P L R V N V D P I L H Q N R T V I I G K L R - G - M S I P S C T K I . . . . . . 1698 ATCACACACGCTCGAAGCATATCTTCCAATACCTGAATGGTCCGCTCAGATTGACCATCT I T H A R S I S S N T - M V R S D - P S S H T L E A Y L P I P E W S A Q I D H L D H T R S K H I F Q Y L N G P L R L T I . . . . . . 1638 GTCTGAGGGTGAAATGCCGTACTCATATCTAACTGAGTACCCAGACCATGTTGTAATGCC V - G - N A V L I S N - V P R P C C N A S E G E M P Y S Y L T E Y P D H V V M P C L R V K C R T H I - L S T Q T M L - C . . . . . . 1578 TTCCAGAAGTGAGAAGTAAATAGTGAACCTCGATCTGATATGATAGAAAGAGGAACTCCA F Q K - E V N S E P R S D M I E R G T P S R S E K - I V N L D L I - - K E E L H L P E V R S K - - T S I - Y D R K R N S . . . . . . 1518 TGTAGTCGTACAATCTGACTGATATATAGTTCGGCTAACTTTTCTGCTGTATACTTTACC C S R T I - L I Y S S A N F S A V Y F T V V V Q S D - Y I V R L T F L L Y T L P M - S Y N L T D I - F G - L F C C I L Y . . . . . . 1458 CGAACCGGGATGAAGTGGGCAGACTTGGTCAGTCTATCAACAACAACCCAAATAGAGTCA R T G M K W A D L V S L S T T T Q I E S E P G - S G Q T W S V Y Q Q Q P K - S H P N R D E V G R L G Q S I N N N P N R V . . . . . . 1398 TAACCACCCACTGTGGTAGGCAAACCCACAACAAAGTCCATAGTAATCCGCTCCCACTTC - P P T V V G K P T T K S I V I R S H F N H P L W - A N P Q Q S P - - S A P T S I T T H C G R Q T H N K V H S N P L P L . . . . . . 1338 CAAGTAGGAATAGGCATCCTCTGAGATACACCCCCTGGCCGCTGGTGCTCACATTTGACC Q V G I G I L - D T P P G R W C S H L T K - E - A S S E I H P L A A G A H I - P P S R N R H P L R Y T P W P L V L T F D . . . . . . 1278 TGCTGGCAAGTCAAACACCTAGAAACAAAGTCTGAAATATCTCTCTTCATCCCACACCAC C W Q V K H L E T K S E I S L F I P H H A G K S N T - K Q S L K Y L S S S H T T L L A S Q T P R N K V - N I S L H P T P . . . . . . 1218 CAGTAATGCTGACTCAGATCATGATACATCTTTGCCGCTCCCGGATGGATGGAATACCGA Q - C - L R S - Y I F A A P G W M E Y R S N A D S D H D T S L P L P D G W N T E P V M L T Q I M I H L C R S R M D G I P . . . . . . 1158 GTACAATGGGCCTCCTCAAGAATCAATCTAATCAAATCGCCTGTCCTGGGCACACAAATT V Q W A S S R I N L I K S P V L G T Q I Y N G P P Q E S I - S N R L S W A H K F S T M G L L K N Q S N Q I A C P G H T N . . . . . . 1098 CTGCCTCCGATCCGCAAGACACCATCAGAATCAAGGACAGCCTCCTTAGCTTCCCCTCTC L P P I R K T P S E S R T A S L A S P L C L R S A R H H Q N Q G Q P P - L P L S S A S D P Q D T I R I K D S L L S F P S . . . . . . 1038 AATACTCTGTCTCGAATGAGACATAATTTTTCGTCATCAAATTGGTGTGCACGAATTTGC N T L S R M R H N F S S S N W C A R I C I L C L E - D I I F R H Q I G V H E F A Q Y S V S N E T - F F V I K L V C T N L . . . . . . 978 TCGACTAAAGAAGACCGAGCCTCAATAAAAGCAATCATCCCCTCACTCTCTTCTGAAATC S T K E D R A S I K A I I P S L S S E I R L K K T E P Q - K Q S S P H S L L K S L D - R R P S L N K S N H P L T L F - N . . . . . . 918 TGCAAGCGGACAAGACTGTTAGCTAATATCTGCACATCTCTAGCCAATGGTCTCTCCATA C K R T R L L A N I C T S L A N G L S I A S G Q D C - L I S A H L - P M V S P - L Q A D K T V S - Y L H I S S Q W S L H . . . . . . 858 ATACTAAGTGCTGCAAGACTCCCCATGCTAGGAGTCTTCCTACTCAGAGCATCGGCCATA I L S A A R L P M L G V F L L R A S A I Y - V L Q D S P C - E S S Y S E H R P - N T K C C K T P H A R S L P T Q S I G H . . . 798 ACATTGGCCTTTCCTGGATGATACAGAAT T L A F P G - Y R H W P F L D D T E N I G L S W M I Q N Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-2-_PGL-1_AGS-1_PPS_1 (1194 778) (frame '1'; 414 bp, 138 residues) 1 YIFAAPGWME YRVQWASSRI NLIKSPVLGT QILPPIRKTP SESRTASLAS PLNTLSRMRH 61 NFSSSNWCAR ICSTKEDRAS IKAIIPSLSS EICKRTRLLA NICTSLANGL SIILSAARLP 121 MLGVFLLRAS AITLAFPG- >C06HBa0016K14.1-2-_PGL-1_AGS-1_PPS_2 (1480 1244) (frame '0'; 234 bp, 78 residues) 1 LFCCILYPNR DEVGRLGQSI NNNPNRVITT HCGRQTHNKV HSNPLPLPSR NRHPLRYTPW 61 PLVLTFDLLA SQTPRNKV- >C06HBa0016K14.1-2-_PGL-1_AGS-1_PPS_3 (1766 1560) (frame '2'; 204 bp, 68 residues) 1 SDNCYYRQTP LRVNVDPILH QNRSHTLEAY LPIPEWSAQI DHLSEGEMPY SYLTEYPDHV 61 VMPSRSEK- PGL 2 (- strand): 4311 3565 AGS-1 (4024 4013,3955 3565) SCR (e 0.750 d 0.711 a 0.000,e 0.777) Exon 1 4024 4013 ( 12 n); score: 0.750 Intron 1 4012 3956 ( 57 n); Pd: 0.711 Pa: 0.000 Exon 2 3955 3565 ( 391 n); score: 0.777 PGS (4024 4013,3955 3565) SGN-U339313+ PGS (3932 3605) SGN-U328762- 3-phase translation of AGS-1 (-strand): . . : . . . . 4024 AACAAAATACGG : ACCACTCAATAACCACACTCTATCAGTTTACACATTCTCAATATCAAC N K I R : T T Q - P H S I S L H I L N I N T K Y G : P L N N H T L S V Y T F S I S T Q N T : D H S I T T L Y Q F T H S Q Y Q . . . . . . 3907 TCAATATTCAACAATCACAGACTTCACAGAGAAACAATCACAAGATCAGCAAAACACATG S I F N N H R L H R E T I T R S A K H M Q Y S T I T D F T E K Q S Q D Q Q N T C L N I Q Q S Q T S Q R N N H K I S K T H . . . . . . 3847 TTCAAGTTCATCAATAATAAGATGTGCAATGTCATGAGATGCAATGTCAAATATCGTGAT F K F I N N K M C N V M R C N V K Y R D S S S S I I R C A M S - D A M S N I V M V Q V H Q - - D V Q C H E M Q C Q I S - . . . . . . 3787 GCATGTCTTTCCTAACGATACACACCCGCTGTCTCTCAGTTCGGGACCCATGGGGGACAT A C L S - R Y T P A V S Q F G T H G G H H V F P N D T H P L S L S S G P M G D I C M S F L T I H T R C L S V R D P W G T . . . . . . 3727 ATCTGTCCATGCATCTGTCGCGGCGCACGACACGACCCTCGATAAGTAGTAACCTTCGCG I C P C I C R G A R H D P R - V V T F A S V H A S V A A H D T T L D K - - P S R Y L S M H L S R R T T R P S I S S N L R . . . . . . 3667 GCGCGTGATACGTCCCTCGAAATATAATATCCATCGCGGCGCGCGATACGACCCTCGAAA A R D T S L E I - Y P S R R A I R P S K R V I R P S K Y N I H R G A R Y D P R K G A - Y V P R N I I S I A A R D T T L E . . . . . 3607 ATAGTACACCATCCTCTTACCACAACAATGTCTCAGATACAAT I V H H P L T T T M S Q I Q - Y T I L L P Q Q C L R Y N N S T P S S Y H N N V S D T Maximal non-overlapping open reading frames (>= 64 codons): none AGS-2 (4311 3973) SCR (e 0.758) Exon 1 4311 3973 ( 339 n); score: 0.758 PGS (4311 3973) SGN-U337987+ 3-phase translation of AGS-2 (-strand): . . . . . . 4311 GTTGTCACGTGCACAAGCCTCTAATGTAAACATTAGAATTGAAACAAAATAGAAGTCTAA V V T C T S L - C K H - N - N K I E V - L S R A Q A S N V N I R I E T K - K S K C H V H K P L M - T L E L K Q N R S L . . . . . . 4251 AATGAAGTTGTCTTTCAAGTAAAAACAAAATCATAAACTGGAGTAGGAAAGTTCGCTGAG N E V V F Q V K T K S - T G V G K F A E M K L S F K - K Q N H K L E - E S S L R K - S C L S S K N K I I N W S R K V R - . . . . . . 4191 ATGACAAACAGCTACCTCACAATCCTCCACAAGATGCCTCGAAAACGAAGAGAATAGAAG M T N S Y L T I L H K M P R K R R E - K - Q T A T S Q S S T R C L E N E E N R R D D K Q L P H N P P Q D A S K T K R I E . . . . . . 4131 GTATCACAAAGATCCAGGCTCGTAACCTACAAAAATTTGTAGAAGCAAGGGGTGAGTACC V S Q R S R L V T Y K N L - K Q G V S T Y H K D P G S - P T K I C R S K G - V P G I T K I Q A R N L Q K F V E A R G E Y . . . . . . 4071 AAACCACGCGGTACCCAACAAGCAAACCTCTAAACACAAGTTAAGTGAACAAAATACGGG K P R G T Q Q A N L - T Q V K - T K Y G N H A V P N K Q T S K H K L S E Q N T G Q T T R Y P T S K P L N T S - V N K I R . . . . 4011 TACTCCTTACACCCTATCTAAACCTCCACGCTACAGCCT Y S L H P I - T S T L Q P T P Y T L S K P P R Y S V L L T P Y L N L H A T A Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-2 (+strand): . . . . . . 3973 AGGCTGTAGCGTGGAGGTTTAGATAGGGTGTAAGGAGTACCCGTATTTTGTTCACTTAAC R L - R G G L D R V - G V P V F C S L N G C S V E V - I G C K E Y P Y F V H L T A V A W R F R - G V R S T R I L F T - . . . . . . 4033 TTGTGTTTAGAGGTTTGCTTGTTGGGTACCGCGTGGTTTGGTACTCACCCCTTGCTTCTA L C L E V C L L G T A W F G T H P L L L C V - R F A C W V P R G L V L T P C F Y L V F R G L L V G Y R V V W Y S P L A S . . . . . . 4093 CAAATTTTTGTAGGTTACGAGCCTGGATCTTTGTGATACCTTCTATTCTCTTCGTTTTCG Q I F V G Y E P G S L - Y L L F S S F S K F L - V T S L D L C D T F Y S L R F R T N F C R L R A W I F V I P S I L F V F . . . . . . 4153 AGGCATCTTGTGGAGGATTGTGAGGTAGCTGTTTGTCATCTCAGCGAACTTTCCTACTCC R H L V E D C E V A V C H L S E L S Y S G I L W R I V R - L F V I S A N F P T P E A S C G G L - G S C L S S Q R T F L L . . . . . . 4213 AGTTTATGATTTTGTTTTTACTTGAAAGACAACTTCATTTTAGACTTCTATTTTGTTTCA S L - F C F Y L K D N F I L D F Y F V S V Y D F V F T - K T T S F - T S I L F Q Q F M I L F L L E R Q L H F R L L F C F . . . . 4273 ATTCTAATGTTTACATTAGAGGCTTGTGCACGTGACAAC I L M F T L E A C A R D N F - C L H - R L V H V T N S N V Y I R G L C T - Q Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Mon Aug 28 21:41:07 2006 ________________________________________________________________________________ Sequence 3: C06HBa0016K14.1-3, from 1 to 5314, both strands analyzed. ... started at: Mon Aug 28 21:41:07 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 1 ******************************************************************************** EST sequence 1 +strand 1295 n (File: SGN-U323476+) 1 AATCGGCCGA ATTGAAAGGG GGTTTCACTT CAAGCCCTTG CTGAGGATTT GCTTCTCCGT 61 TCGGGCGGCG CTCTCTTCAG GTCCGTTTTA CTGTTCTTGA GGATTGCTTA CCACTAGCAG 121 AGGAAACTTT CAGGCAAGCG ATAAAGAAAA ACTATTACAC CAGGAGCAAG TTCCGTTGCC 181 AATTATCCTC TGAACAGGTG AAGGACCTGT GCAAGCTTTT CAAGACTGCG GTCAAAGGGT 241 CCAGGTCCAA TGAGACACAG CTCAGACTTG AGACCAATGT GGTACCCAAA CGAGACAGAG 301 CTAAGAGGCA TGGTCTGGAT GAAAGTAGAC GTCCTGATCG CGCTCGACAA TTTGAGCAGG 361 TTGAAGATCG TCGGTACCGC GAGCAGGTTG AAGATCGTCG GTACCGCGAG CAGGCTGAAG 421 ATCGTCGGTA CCGCGAGCAG GCTGAAGATC GTCTGTACCG TGAGCAGGTT GAAGATCGTG 481 GGTACCGTGA ACATCCTCAT TTGCATGGAA GGGGCTTGAA TACATCTACT TTGATTCCAT 541 TAGCCCCTCT TCGACCTTTA CCACCACTGG TTCAATCTTA TGCATATGAC AGGACTTTGG 601 GAAGAGATCC ATATGGACGG GACACAGTGA TACCGCATAA TGATTCTTAC AGGCAGAGGC 661 GATTGGTGGA ACTAGCTGAT CCATATAGGC GGGACACTGT GATAGGCAAT CCGGATGTTC 721 ACAGAGGACG AGCATTGTTA GAGTCACATG ATCACTACAG ACGGGAGGGA ATTTCAGAGC 781 CTCGTGAGTA TCGTCAGCCT CTGAATTTGG AAACTAGACT CCAGGTTGCT GGTGGGATTA 841 ATGATTCATA TGTGCCATAC CGTGAGCGGC TATCATACCA TGATCCTCCT GTTAACTCAG 901 TGCGCTCCCA GCCCGAGTTT AACCCCCCTC CAGCTGGTTT GCGATCAGAA TACCTCCATG 961 GTGGTACATC TACTGTGTTC AGTTCTGCCA GAAGCATGCT TCCTGAGTAC CCTTCATCTT 1021 CAGCGGGCCC TCTGTACAGG CACCCTCATC GACGTGAACA TCGTTTTTAG GTTTACAAAA 1081 AGGTAACCAT CACATGTGGT TCTGGTAATC AGAATCATGC ACAGCTTAAA ACTTGAGACT 1141 ATCTAGAATG TTTTCAGAAT CCATTGGGGT TTCTGTTTTA TTTTGTTTAA ACTATATTAG 1201 ATATTACTTC TCCATTCTTG CTGTAAGCAA GAGATTTAAA AAAAAAAAAT TGAAAAAATT 1261 TTTGGGGGCG GGCCGGGATC CCATTTCCCC TTTTA Predicted gene structure (within gDNA segment 5314 to 3109): Exon 1 5314 4290 (1025 n); cDNA 213 1237 (1025 n); score: 0.994 MATCH C06HBa0016K14.1-3- SGN-U323476+ 0.994 1025 0.792 C PGS_C06HBa0016K14.1-3-_SGN-U323476+ (5314 4290) Alignment (genomic DNA sequence = upper lines): AAGCTTTTCA AGACTGCGGT CAAAGGGTCC AGGTCCAATG AGACACAGCT CAGACTTGAG 5255 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCTTTTCA AGACTGCGGT CAAAGGGTCC AGGTCCAATG AGACACAGCT CAGACTTGAG 272 ACCAATGTGG TACCCAAACG AGACAGAGCT AAGAGGCATG GTCTGGATGA AAGTAGACGT 5195 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAATGTGG TACCCAAACG AGACAGAGCT AAGAGGCATG GTCTGGATGA AAGTAGACGT 332 CCTGATCGCG CTCGACAATT TGAGCAGGTT GAAGATCGTC GGTACCGCGA GCAGGCTGAA 5135 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| |||| CCTGATCGCG CTCGACAATT TGAGCAGGTT GAAGATCGTC GGTACCGCGA GCAGGTTGAA 392 GATCGTCGGT ACCGCGAGCA GGCTGAAGAT CGTTGGTACC GCGAGCAGGC TGAAGATCGT 5075 |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| |||||||||| GATCGTCGGT ACCGCGAGCA GGCTGAAGAT CGTCGGTACC GCGAGCAGGC TGAAGATCGT 452 CTGTACCGTG AGCAGGTTGA AGATCGTGGG TACCGTGAAC ATCCTCATTT GCATGGAAGG 5015 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTACCGTG AGCAGGTTGA AGATCGTGGG TACCGTGAAC ATCCTCATTT GCATGGAAGG 512 GGCTTGAATA CATCTACTTT GATTCCATTA GCCCCTCTTC GACCTTTACC ACCACTTGTT 4955 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| ||| GGCTTGAATA CATCTACTTT GATTCCATTA GCCCCTCTTC GACCTTTACC ACCACTGGTT 572 CAATCTTATG CATATGACAG GACTTTGGGA AGAGATCCTT ATGGACGGGA CACAGTGATA 4895 |||||||||| |||||||||| |||||||||| |||||||| | |||||||||| |||||||||| CAATCTTATG CATATGACAG GACTTTGGGA AGAGATCCAT ATGGACGGGA CACAGTGATA 632 CCGCATAATG ATTCTTACAG GCAGAGGCGA TTGGTGGAAC TAGCTGATCC TTATAGGCGG 4835 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| CCGCATAATG ATTCTTACAG GCAGAGGCGA TTGGTGGAAC TAGCTGATCC ATATAGGCGG 692 GACACTGTGA TAGGCAATCC GGATGTTCAC AGAGGACGAG CATTGTTAGA GTCACATGAT 4775 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACACTGTGA TAGGCAATCC GGATGTTCAC AGAGGACGAG CATTGTTAGA GTCACATGAT 752 CACTACAGAC GGGAGGGAAT TTCAGAGCCT CGTGAGTATC GTCAGCCTCT GAATTTGGAA 4715 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACTACAGAC GGGAGGGAAT TTCAGAGCCT CGTGAGTATC GTCAGCCTCT GAATTTGGAA 812 ACTAGACTCC AGGTTGCTGG TGGGATTAAT GATTCATATG TGCCATACCG TGAGCGGCTA 4655 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTAGACTCC AGGTTGCTGG TGGGATTAAT GATTCATATG TGCCATACCG TGAGCGGCTA 872 TCATACCATG ATCTTCCTGT TAACTCAGTG CGCTCCCAGC CCGAGTTTAA CCCCCCTCCA 4595 |||||||||| ||| |||||| |||||||||| |||||||||| |||||||||| |||||||||| TCATACCATG ATCCTCCTGT TAACTCAGTG CGCTCCCAGC CCGAGTTTAA CCCCCCTCCA 932 GCTGGTTTGC GATCAGAATA CCTCCATGGT GGTACATCTA CTGTGTTCAG TTCTGCCAGA 4535 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTGGTTTGC GATCAGAATA CCTCCATGGT GGTACATCTA CTGTGTTCAG TTCTGCCAGA 992 AGCATGCTTC CTGAGTACCC TTCATCTTCA GCGGGCCCTC TGTACAGGCA CCCTCATCGA 4475 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCATGCTTC CTGAGTACCC TTCATCTTCA GCGGGCCCTC TGTACAGGCA CCCTCATCGA 1052 CGTGAACATC GTTTTTAGGT TTACAAAAAG GTAACCATCA CATGTGGTTC TGGTAATCAG 4415 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGTGAACATC GTTTTTAGGT TTACAAAAAG GTAACCATCA CATGTGGTTC TGGTAATCAG 1112 AATCATGCAC AGCTTAAAAC TTGAGACTAT CTAGAATGTT TTCAGAATCC ATTGGGGTTT 4355 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATCATGCAC AGCTTAAAAC TTGAGACTAT CTAGAATGTT TTCAGAATCC ATTGGGGTTT 1172 CTGTTTTATT TTGTTTAAAC TATATTAGAT ATTACTTCTC CATTCTTGCT GTAAGCAAGA 4295 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTTTATT TTGTTTAAAC TATATTAGAT ATTACTTCTC CATTCTTGCT GTAAGCAAGA 1232 GATTT 4290 ||||| GATTT 1237 hqPGS_C06HBa0016K14.1-3-_SGN-U323476+ (5314 4290) Total number of EST alignments reported: 1 ________________________________________________________________________________ Predicted gene locations (1) in segment 1 to 5314: PGL 1 (- strand): 5314 4290 AGS-1 (5314 4290) SCR (e 0.994) Exon 1 5314 4290 (1025 n); score: 0.994 PGS (5314 4290) SGN-U323476+ 3-phase translation of AGS-1 (-strand): . . . . . . 5314 AAGCTTTTCAAGACTGCGGTCAAAGGGTCCAGGTCCAATGAGACACAGCTCAGACTTGAG K L F K T A V K G S R S N E T Q L R L E S F S R L R S K G P G P M R H S S D L R A F Q D C G Q R V Q V Q - D T A Q T - . . . . . . 5254 ACCAATGTGGTACCCAAACGAGACAGAGCTAAGAGGCATGGTCTGGATGAAAGTAGACGT T N V V P K R D R A K R H G L D E S R R P M W Y P N E T E L R G M V W M K V D V D Q C G T Q T R Q S - E A W S G - K - T . . . . . . 5194 CCTGATCGCGCTCGACAATTTGAGCAGGTTGAAGATCGTCGGTACCGCGAGCAGGCTGAA P D R A R Q F E Q V E D R R Y R E Q A E L I A L D N L S R L K I V G T A S R L K S - S R S T I - A G - R S S V P R A G - . . . . . . 5134 GATCGTCGGTACCGCGAGCAGGCTGAAGATCGTTGGTACCGCGAGCAGGCTGAAGATCGT D R R Y R E Q A E D R W Y R E Q A E D R I V G T A S R L K I V G T A S R L K I V R S S V P R A G - R S L V P R A G - R S . . . . . . 5074 CTGTACCGTGAGCAGGTTGAAGATCGTGGGTACCGTGAACATCCTCATTTGCATGGAAGG L Y R E Q V E D R G Y R E H P H L H G R C T V S R L K I V G T V N I L I C M E G S V P - A G - R S W V P - T S S F A W K . . . . . . 5014 GGCTTGAATACATCTACTTTGATTCCATTAGCCCCTCTTCGACCTTTACCACCACTTGTT G L N T S T L I P L A P L R P L P P L V A - I H L L - F H - P L F D L Y H H L F G L E Y I Y F D S I S P S S T F T T T C . . . . . . 4954 CAATCTTATGCATATGACAGGACTTTGGGAAGAGATCCTTATGGACGGGACACAGTGATA Q S Y A Y D R T L G R D P Y G R D T V I N L M H M T G L W E E I L M D G T Q - Y S I L C I - Q D F G K R S L W T G H S D . . . . . . 4894 CCGCATAATGATTCTTACAGGCAGAGGCGATTGGTGGAACTAGCTGATCCTTATAGGCGG P H N D S Y R Q R R L V E L A D P Y R R R I M I L T G R G D W W N - L I L I G G T A - - F L Q A E A I G G T S - S L - A . . . . . . 4834 GACACTGTGATAGGCAATCCGGATGTTCACAGAGGACGAGCATTGTTAGAGTCACATGAT D T V I G N P D V H R G R A L L E S H D T L - - A I R M F T E D E H C - S H M I G H C D R Q S G C S Q R T S I V R V T - . . . . . . 4774 CACTACAGACGGGAGGGAATTTCAGAGCCTCGTGAGTATCGTCAGCCTCTGAATTTGGAA H Y R R E G I S E P R E Y R Q P L N L E T T D G R E F Q S L V S I V S L - I W K S L Q T G G N F R A S - V S S A S E F G . . . . . . 4714 ACTAGACTCCAGGTTGCTGGTGGGATTAATGATTCATATGTGCCATACCGTGAGCGGCTA T R L Q V A G G I N D S Y V P Y R E R L L D S R L L V G L M I H M C H T V S G Y N - T P G C W W D - - F I C A I P - A A . . . . . . 4654 TCATACCATGATCTTCCTGTTAACTCAGTGCGCTCCCAGCCCGAGTTTAACCCCCCTCCA S Y H D L P V N S V R S Q P E F N P P P H T M I F L L T Q C A P S P S L T P L Q I I P - S S C - L S A L P A R V - P P S . . . . . . 4594 GCTGGTTTGCGATCAGAATACCTCCATGGTGGTACATCTACTGTGTTCAGTTCTGCCAGA A G L R S E Y L H G G T S T V F S S A R L V C D Q N T S M V V H L L C S V L P E S W F A I R I P P W W Y I Y C V Q F C Q . . . . . . 4534 AGCATGCTTCCTGAGTACCCTTCATCTTCAGCGGGCCCTCTGTACAGGCACCCTCATCGA S M L P E Y P S S S A G P L Y R H P H R A C F L S T L H L Q R A L C T G T L I D K H A S - V P F I F S G P S V Q A P S S . . . . . . 4474 CGTGAACATCGTTTTTAGGTTTACAAAAAGGTAACCATCACATGTGGTTCTGGTAATCAG R E H R F - V Y K K V T I T C G S G N Q V N I V F R F T K R - P S H V V L V I R T - T S F L G L Q K G N H H M W F W - S . . . . . . 4414 AATCATGCACAGCTTAAAACTTGAGACTATCTAGAATGTTTTCAGAATCCATTGGGGTTT N H A Q L K T - D Y L E C F Q N P L G F I M H S L K L E T I - N V F R I H W G F E S C T A - N L R L S R M F S E S I G V . . . . . . 4354 CTGTTTTATTTTGTTTAAACTATATTAGATATTACTTCTCCATTCTTGCTGTAAGCAAGA L F Y F V - T I L D I T S P F L L - A R C F I L F K L Y - I L L L H S C C K Q E S V L F C L N Y I R Y Y F S I L A V S K . 4294 GATTT D I R F Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-3-_PGL-1_AGS-1_PPS_1 (5314 4457) (frame '1'; 855 bp, 285 residues) 1 KLFKTAVKGS RSNETQLRLE TNVVPKRDRA KRHGLDESRR PDRARQFEQV EDRRYREQAE 61 DRRYREQAED RWYREQAEDR LYREQVEDRG YREHPHLHGR GLNTSTLIPL APLRPLPPLV 121 QSYAYDRTLG RDPYGRDTVI PHNDSYRQRR LVELADPYRR DTVIGNPDVH RGRALLESHD 181 HYRREGISEP REYRQPLNLE TRLQVAGGIN DSYVPYRERL SYHDLPVNSV RSQPEFNPPP 241 AGLRSEYLHG GTSTVFSSAR SMLPEYPSSS AGPLYRHPHR REHRF- 3-phase translation of AGS-1 (+strand): . . . . . . 4290 AAATCTCTTGCTTACAGCAAGAATGGAGAAGTAATATCTAATATAGTTTAAACAAAATAA K S L A Y S K N G E V I S N I V - T K - N L L L T A R M E K - Y L I - F K Q N K I S C L Q Q E W R S N I - Y S L N K I . . . . . . 4350 AACAGAAACCCCAATGGATTCTGAAAACATTCTAGATAGTCTCAAGTTTTAAGCTGTGCA N R N P N G F - K H S R - S Q V L S C A T E T P M D S E N I L D S L K F - A V H K Q K P Q W I L K T F - I V S S F K L C . . . . . . 4410 TGATTCTGATTACCAGAACCACATGTGATGGTTACCTTTTTGTAAACCTAAAAACGATGT - F - L P E P H V M V T F L - T - K R C D S D Y Q N H M - W L P F C K P K N D V M I L I T R T T C D G Y L F V N L K T M . . . . . . 4470 TCACGTCGATGAGGGTGCCTGTACAGAGGGCCCGCTGAAGATGAAGGGTACTCAGGAAGC S R R - G C L Y R G P A E D E G Y S G S H V D E G A C T E G P L K M K G T Q E A F T S M R V P V Q R A R - R - R V L R K . . . . . . 4530 ATGCTTCTGGCAGAACTGAACACAGTAGATGTACCACCATGGAGGTATTCTGATCGCAAA M L L A E L N T V D V P P W R Y S D R K C F W Q N - T Q - M Y H H G G I L I A N H A S G R T E H S R C T T M E V F - S Q . . . . . . 4590 CCAGCTGGAGGGGGGTTAAACTCGGGCTGGGAGCGCACTGAGTTAACAGGAAGATCATGG P A G G G L N S G W E R T E L T G R S W Q L E G G - T R A G S A L S - Q E D H G T S W R G V K L G L G A H - V N R K I M . . . . . . 4650 TATGATAGCCGCTCACGGTATGGCACATATGAATCATTAATCCCACCAGCAACCTGGAGT Y D S R S R Y G T Y E S L I P P A T W S M I A A H G M A H M N H - S H Q Q P G V V - - P L T V W H I - I I N P T S N L E . . . . . . 4710 CTAGTTTCCAAATTCAGAGGCTGACGATACTCACGAGGCTCTGAAATTCCCTCCCGTCTG L V S K F R G - R Y S R G S E I P S R L - F P N S E A D D T H E A L K F P P V C S S F Q I Q R L T I L T R L - N S L P S . . . . . . 4770 TAGTGATCATGTGACTCTAACAATGCTCGTCCTCTGTGAACATCCGGATTGCCTATCACA - - S C D S N N A R P L - T S G L P I T S D H V T L T M L V L C E H P D C L S Q V V I M - L - Q C S S S V N I R I A Y H . . . . . . 4830 GTGTCCCGCCTATAAGGATCAGCTAGTTCCACCAATCGCCTCTGCCTGTAAGAATCATTA V S R L - G S A S S T N R L C L - E S L C P A Y K D Q L V P P I A S A C K N H Y S V P P I R I S - F H Q S P L P V R I I . . . . . . 4890 TGCGGTATCACTGTGTCCCGTCCATAAGGATCTCTTCCCAAAGTCCTGTCATATGCATAA C G I T V S R P - G S L P K V L S Y A - A V S L C P V H K D L F P K S C H M H K M R Y H C V P S I R I S S Q S P V I C I . . . . . . 4950 GATTGAACAAGTGGTGGTAAAGGTCGAAGAGGGGCTAATGGAATCAAAGTAGATGTATTC D - T S G G K G R R G A N G I K V D V F I E Q V V V K V E E G L M E S K - M Y S R L N K W W - R S K R G - W N Q S R C I . . . . . . 5010 AAGCCCCTTCCATGCAAATGAGGATGTTCACGGTACCCACGATCTTCAACCTGCTCACGG K P L P C K - G C S R Y P R S S T C S R S P F H A N E D V H G T H D L Q P A H G Q A P S M Q M R M F T V P T I F N L L T . . . . . . 5070 TACAGACGATCTTCAGCCTGCTCGCGGTACCAACGATCTTCAGCCTGCTCGCGGTACCGA Y R R S S A C S R Y Q R S S A C S R Y R T D D L Q P A R G T N D L Q P A R G T D V Q T I F S L L A V P T I F S L L A V P . . . . . . 5130 CGATCTTCAGCCTGCTCGCGGTACCGACGATCTTCAACCTGCTCAAATTGTCGAGCGCGA R S S A C S R Y R R S S T C S N C R A R D L Q P A R G T D D L Q P A Q I V E R D T I F S L L A V P T I F N L L K L S S A . . . . . . 5190 TCAGGACGTCTACTTTCATCCAGACCATGCCTCTTAGCTCTGTCTCGTTTGGGTACCACA S G R L L S S R P C L L A L S R L G T T Q D V Y F H P D H A S - L C L V W V P H I R T S T F I Q T M P L S S V S F G Y H . . . . . . 5250 TTGGTCTCAAGTCTGAGCTGTGTCTCATTGGACCTGGACCCTTTGACCGCAGTCTTGAAA L V S S L S C V S L D L D P L T A V L K W S Q V - A V S H W T W T L - P Q S - K I G L K S E L C L I G P G P F D R S L E . 5310 AGCTT S A K L Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-3+_PGL-1_AGS-1_PPS_1 (4988 5314) (frame '0'; 327 bp, 109 residues) 1 WNQSRCIQAP SMQMRMFTVP TIFNLLTVQT IFSLLAVPTI FSLLAVPTIF SLLAVPTIFN 61 LLKLSSAIRT STFIQTMPLS SVSFGYHIGL KSELCLIGPG PFDRSLEKL >C06HBa0016K14.1-3+_PGL-1_AGS-1_PPS_2 (4714 5001) (frame '2'; 285 bp, 95 residues) 1 FPNSEADDTH EALKFPPVCS DHVTLTMLVL CEHPDCLSQC PAYKDQLVPP IASACKNHYA 61 VSLCPVHKDL FPKSCHMHKI EQVVVKVEEG LMESK- >C06HBa0016K14.1-3+_PGL-1_AGS-1_PPS_3 (4482 4733) (frame '1'; 249 bp, 83 residues) 1 GCLYRGPAED EGYSGSMLLA ELNTVDVPPW RYSDRKPAGG GLNSGWERTE LTGRSWYDSR 61 SRYGTYESLI PPATWSLVSK FRG- ... finished at: Mon Aug 28 21:41:12 2006 ________________________________________________________________________________ Sequence 4: C06HBa0016K14.1-4, from 1 to 15924, both strands analyzed. ... started at: Mon Aug 28 21:41:12 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 1 ... matches indexed, elapsed seconds = 1 HitsTableSize = 8 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 7 ******************************************************************************** EST sequence 7 +strand 1909 n (File: SGN-U319927+) 1 TCTTTCACTA CCCTACATTT AACATTTTCC TATAACCACT GCTTTAGATA AGTCAAATTT 61 AGCTCTTTGT TTTGATCTCT GTTTCAAAAG AAAACACCTA TTAAGCAGCC ATCATCTTTC 121 TTATCTTTTC CAAAACCAAA ACTACTGACT TTTCTTGAAA AAAGAAGAGG TGGGGTGCTT 181 TCTTTTCTTC AAAAACCTTC TCTTTGTTCT TGAAAAAACA GGACTCATTC ATTTTTTTTT 241 GTGTGTTTCT TTCAGAAGAA ATAACAAAGA CCCTTTCTCT GTTTTCTTCA TATTTCAGCT 301 TTGAGCTACT TGGATCTGTT TTTTTTTTTT TGAATATACA AGTAGTTTGT GTGTTCTGGG 361 GTCTACAGAA GAAGGAGAAG CTAAAAGGGG TGATTTTGTT TTTTGTTTGT TGTTGTTCTA 421 ATGGGGATAT GTTTGAGTGC TAGAATTAAA GCTGAAAGCC CATTTCATAC AGGGCTAAAT 481 TCAAGATCTG TTAGCATAGA TGGTGGTGAT AGTAATTCAA GCTCCAGGGT TCCATCAACT 541 ACTAGAAGTG AAGGAGAGAT ATTGCAGTCT CCCAATTTGA AGAGCTTTAG TTTTTCTGAT 601 CTCAGAACAG CCACTAGGAA CTTTCGTCCC GATAGTGTGC TGGGAGAAGG TGGATTTGGA 661 TCAGTTTTTA AAGGGTGGAT CGATGAGAAC ACGTTCGCAG CTACCAAGCC TGGGACAGGT 721 GTGATCATTG CTGTGAAAAG GCTTAATCAA GAAGGTTTTC AAGGTCACAG GGAATGGCTG 781 GCAGAAGTTA ACTATTTGGG GCAGTTTTTT CATCCTCATC TTGTAAAGCT GATTGGCTAC 841 TGCTTGGAGG ACGAACATCG TCTTTTGGTT TATGAGTTCA TGCCTCGGGG CAGCTTGGAA 901 AACCATTTGT TTAGGAGAGG TTCTTATTTC CAGCCTCTTT CTTGGAAACT ACGCTTGAAG 961 GTTGCTCTAG GAGCTGCAAA AGGGCTTGCT TTTCTCCACA GTGCTGAGAC TAAAGTGATA 1021 TATCGAGACT TCAAAACCTC GAATATTTTG CTTGATTCGA ACTACACTGC CAAACTCTCA 1081 GACTTTGGAT TAGCCAAGGA TGGGCCAACA GGCGATAAAA GCCATGTCTC TACAAGGGTT 1141 ATGGGGACCT ATGGATATGC CGCTCCAGAA TACATGGCTA CTGGTCATTT AACTAGCAAG 1201 AGTGATGTGT ATAGTTTTGG AGTTGTCCTC CTTGAAATGC TGTCAGGTCG TAGGGCAATC 1261 GACAAGAATA GACCATCCGG AGAACACAAT CTAGTGGAGT GGGCGAAACC TTACCTAGGT 1321 AACAAACGTA AGGTCTTCCG TGTTCTGGAT ACCCGTCTTG AAGGCCAGTA TTCAATGGAG 1381 GTGGCGAGTA AAGTAGCTAA CCTTGCCCTG CGGTGCCTAT CCAAAGATCC TCGGTTTAGA 1441 CCAAGCATGA GTGACATTGT TAAAGAACTG GAGCAACTTT ATCAACAATC TAAAGATTCA 1501 GGAAATACTC GCAGCCACGC TAGCAACCGA CCTAGACCAC GTAGACGAAG TGCTGGTGAT 1561 GTTGCTAATA GAAATCCTTC AGTTGCTTAT CCGAGACCGT CTGCTTCTCC CCTTTACGCT 1621 AAATAATTAA ACAAATGATT GAAAGATGCC TTAATTTCTT GTTTTTTTTG TCTTATATGT 1681 GTTTATCAAG CTTGATAAGT AGTTGAGGCT GATGAAGAAC CTCTGCACAT TTGCAGTATC 1741 AGCTGGCTGA TTGTACAGTT GTTTTTGGCT AATGTATTGA ATCTATACTT TCTGTAGGTC 1801 CTGAATGTGT TTGTAACTTT GAGTTTTGCT CACAGCTCAC TATATGTTCA CTTTTTGACA 1861 CTAAAGGAAT GCATACTACC CTTTCATACT GAAAAAAAAA AAAAAAAAA Predicted gene structure (within gDNA segment 1208 to 5891): Exon 1 1808 2279 ( 472 n); cDNA 1 472 ( 472 n); score: 1.000 Intron 1 2280 2657 ( 378 n); Pd: 0.992 (s: 1.00), Pa: 0.943 (s: 1.00) Exon 2 2658 2965 ( 308 n); cDNA 473 780 ( 308 n); score: 1.000 Intron 2 2966 3353 ( 388 n); Pd: 0.944 (s: 1.00), Pa: 0.981 (s: 0.98) Exon 3 3354 3489 ( 136 n); cDNA 781 916 ( 136 n); score: 0.993 Intron 3 3490 3574 ( 85 n); Pd: 0.964 (s: 1.00), Pa: 0.967 (s: 1.00) Exon 4 3575 3717 ( 143 n); cDNA 917 1059 ( 143 n); score: 1.000 Intron 4 3718 3817 ( 100 n); Pd: 0.846 (s: 1.00), Pa: 0.970 (s: 1.00) Exon 5 3818 3941 ( 124 n); cDNA 1060 1183 ( 124 n); score: 1.000 Intron 5 3942 4384 ( 443 n); Pd: 0.999 (s: 1.00), Pa: 0.969 (s: 1.00) Exon 6 4385 5091 ( 707 n); cDNA 1184 1890 ( 707 n); score: 1.000 PPA cDNA 1892 1909 MATCH C06HBa0016K14.1-4+ SGN-U319927+ 0.999 1890 0.990 C PGS_C06HBa0016K14.1-4+_SGN-U319927+ (1808 2279,2658 2965,3354 3489,3575 3717,3818 3941,4385 5091) Alignment (genomic DNA sequence = upper lines): TCTTTCACTA CCCTACATTT AACATTTTCC TATAACCACT GCTTTAGATA AGTCAAATTT 1867 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTTCACTA CCCTACATTT AACATTTTCC TATAACCACT GCTTTAGATA AGTCAAATTT 60 AGCTCTTTGT TTTGATCTCT GTTTCAAAAG AAAACACCTA TTAAGCAGCC ATCATCTTTC 1927 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTCTTTGT TTTGATCTCT GTTTCAAAAG AAAACACCTA TTAAGCAGCC ATCATCTTTC 120 TTATCTTTTC CAAAACCAAA ACTACTGACT TTTCTTGAAA AAAGAAGAGG TGGGGTGCTT 1987 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTATCTTTTC CAAAACCAAA ACTACTGACT TTTCTTGAAA AAAGAAGAGG TGGGGTGCTT 180 TCTTTTCTTC AAAAACCTTC TCTTTGTTCT TGAAAAAACA GGACTCATTC ATTTTTTTTT 2047 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTTTCTTC AAAAACCTTC TCTTTGTTCT TGAAAAAACA GGACTCATTC ATTTTTTTTT 240 GTGTGTTTCT TTCAGAAGAA ATAACAAAGA CCCTTTCTCT GTTTTCTTCA TATTTCAGCT 2107 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGTGTTTCT TTCAGAAGAA ATAACAAAGA CCCTTTCTCT GTTTTCTTCA TATTTCAGCT 300 TTGAGCTACT TGGATCTGTT TTTTTTTTTT TGAATATACA AGTAGTTTGT GTGTTCTGGG 2167 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGAGCTACT TGGATCTGTT TTTTTTTTTT TGAATATACA AGTAGTTTGT GTGTTCTGGG 360 GTCTACAGAA GAAGGAGAAG CTAAAAGGGG TGATTTTGTT TTTTGTTTGT TGTTGTTCTA 2227 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTACAGAA GAAGGAGAAG CTAAAAGGGG TGATTTTGTT TTTTGTTTGT TGTTGTTCTA 420 ATGGGGATAT GTTTGAGTGC TAGAATTAAA GCTGAAAGCC CATTTCATAC AGGTATTTGA 2287 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || ATGGGGATAT GTTTGAGTGC TAGAATTAAA GCTGAAAGCC CATTTCATAC AG........ 472 TATAAGACTA TGAACTCTTT TTTTTCTTCT TGTTTACTTG CACAATTTTT GCAAATTTGA 2347 .......... .......... .......... .......... .......... .......... 472 GTCTTTTTGG AGGAGGGGTT CTGTAGAATG GTTTTGTTTG TTTGTTTTCT AATTTTCATT 2407 .......... .......... .......... .......... .......... .......... 472 CATATTAGGA TGATCATGCC TTTGTTCATG TAAAGTGGCT AGTTTGAGTT CTTGCTAATT 2467 .......... .......... .......... .......... .......... .......... 472 TTTTAGTAGT CTCTATTTGG TAATAGTAGT TATCCTGATT AATATATCTT GTTGATAAAA 2527 .......... .......... .......... .......... .......... .......... 472 AAAAATTGGG GGTAGGGGTA GCAAAGGGGA TTATAAGTTA GTTAATTGAG CTACTAAGAT 2587 .......... .......... .......... .......... .......... .......... 472 TTAAGATGTT TTGTTGTGTT CTAATGGGGT TAATTGATGG TAATCTAATA AATTGAGAGA 2647 .......... .......... .......... .......... .......... .......... 472 ATTATTGCAG GGCTAAATTC AAGATCTGTT AGCATAGATG GTGGTGATAG TAATTCAAGC 2707 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... GGCTAAATTC AAGATCTGTT AGCATAGATG GTGGTGATAG TAATTCAAGC 522 TCCAGGGTTC CATCAACTAC TAGAAGTGAA GGAGAGATAT TGCAGTCTCC CAATTTGAAG 2767 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCAGGGTTC CATCAACTAC TAGAAGTGAA GGAGAGATAT TGCAGTCTCC CAATTTGAAG 582 AGCTTTAGTT TTTCTGATCT CAGAACAGCC ACTAGGAACT TTCGTCCCGA TAGTGTGCTG 2827 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTTTAGTT TTTCTGATCT CAGAACAGCC ACTAGGAACT TTCGTCCCGA TAGTGTGCTG 642 GGAGAAGGTG GATTTGGATC AGTTTTTAAA GGGTGGATCG ATGAGAACAC GTTCGCAGCT 2887 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAGAAGGTG GATTTGGATC AGTTTTTAAA GGGTGGATCG ATGAGAACAC GTTCGCAGCT 702 ACCAAGCCTG GGACAGGTGT GATCATTGCT GTGAAAAGGC TTAATCAAGA AGGTTTTCAA 2947 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAAGCCTG GGACAGGTGT GATCATTGCT GTGAAAAGGC TTAATCAAGA AGGTTTTCAA 762 GGTCACAGGG AATGGCTGGT GAGTTTTTTT TTTTTATGTT TGATATGGAG ATGTTCGAGT 3007 |||||||||| |||||||| GGTCACAGGG AATGGCTG.. .......... .......... .......... .......... 780 CGGTTTAAGC TTAAAGTGCA TTGATTATAC CATGAACTCA GAGTAAGCGG AACTAGATCA 3067 .......... .......... .......... .......... .......... .......... 780 TTTGCTATTG GTTTGGACGA AGCCAGTAGC TTTTGCTTAG TCCCTGTATT TGTATTAGGA 3127 .......... .......... .......... .......... .......... .......... 780 AATTCATTAA ATATGTATGA AACGTTTTAT TGCGAAACCT ACTAAAGAAG ATGAATTATG 3187 .......... .......... .......... .......... .......... .......... 780 AGATTTGAGG CAAGTTCAGA ACCCATAAAC TTCAAATCTT GATTCTGCCT CTACAAGAAT 3247 .......... .......... .......... .......... .......... .......... 780 TGTCGTTGTT ACTTATTGAG TGCTTTATTA AGTGCTAATA CTTTGTTATT TTGAGTGTCA 3307 .......... .......... .......... .......... .......... .......... 780 TAAGGTTGTC TGAGTTCTGA TTTGTATTAT GCTTCTATGT TTTTAGGCAG AAGTTAACTA 3367 |||| |||||||||| .......... .......... .......... .......... ......GCAG AAGTTAACTA 794 TTTGGGGCAG TTTTCTCATC CTCATCTTGT AAAGCTGATT GGCTACTGCT TGGAGGACGA 3427 |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGGGGCAG TTTTTTCATC CTCATCTTGT AAAGCTGATT GGCTACTGCT TGGAGGACGA 854 ACATCGTCTT TTGGTTTATG AGTTCATGCC TCGGGGCAGC TTGGAAAACC ATTTGTTTAG 3487 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATCGTCTT TTGGTTTATG AGTTCATGCC TCGGGGCAGC TTGGAAAACC ATTTGTTTAG 914 GAGTGAGTAC TTTGCCCCTA CCATCTTAAT TTCAACTCTA TGATCATCGA CACCTTTGGA 3547 || GA........ .......... .......... .......... .......... .......... 916 GTAATAGTGT TTGTTGTTCT GTAACAGGAG GTTCTTATTT CCAGCCTCTT TCTTGGAAAC 3607 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......GAG GTTCTTATTT CCAGCCTCTT TCTTGGAAAC 949 TACGCTTGAA GGTTGCTCTA GGAGCTGCAA AAGGGCTTGC TTTTCTCCAC AGTGCTGAGA 3667 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACGCTTGAA GGTTGCTCTA GGAGCTGCAA AAGGGCTTGC TTTTCTCCAC AGTGCTGAGA 1009 CTAAAGTGAT ATATCGAGAC TTCAAAACCT CGAATATTTT GCTTGATTCG GTATGTGTCC 3727 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTAAAGTGAT ATATCGAGAC TTCAAAACCT CGAATATTTT GCTTGATTCG .......... 1059 CCTATGAATT AGATCTTTGT GCAATTGCTT GCTTATTACG AACCAGCTAA CGTGTGCTTA 3787 .......... .......... .......... .......... .......... .......... 1059 CTTGGTGCTG TTTCTCTTTT ATCGTTATAG AACTACACTG CCAAACTCTC AGACTTTGGA 3847 |||||||||| |||||||||| |||||||||| .......... .......... .......... AACTACACTG CCAAACTCTC AGACTTTGGA 1089 TTAGCCAAGG ATGGGCCAAC AGGCGATAAA AGCCATGTCT CTACAAGGGT TATGGGGACC 3907 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAGCCAAGG ATGGGCCAAC AGGCGATAAA AGCCATGTCT CTACAAGGGT TATGGGGACC 1149 TATGGATATG CCGCTCCAGA ATACATGGCT ACTGGTATGG TTTCTTTTCC CCCCTGACTC 3967 |||||||||| |||||||||| |||||||||| |||| TATGGATATG CCGCTCCAGA ATACATGGCT ACTG...... .......... .......... 1183 ATGTGTTAGT TTTCTCTCTT GTTCCAACGT GGCCCGACAT CCATAGAGTA TCCAAATTTA 4027 .......... .......... .......... .......... .......... .......... 1183 GAATATTAGG TACTGATCAG GACATCTTTA CGTCTAAGAT GGAACATTTT TTCTTATCCA 4087 .......... .......... .......... .......... .......... .......... 1183 AGATCCATCT AACATGCAGT TTTCTCTTTT CTTTTTAACT CGCATTCTTA TTGTCAAGAA 4147 .......... .......... .......... .......... .......... .......... 1183 CCTCATTAGT CATTCACCTA TGATGCATTT GTACTGCACT CCATCCCCTG GGCAACAATT 4207 .......... .......... .......... .......... .......... .......... 1183 AGTTACTGCT CTTTCTGGGA TGGACTTTGA TATCTTCATG TTGAGTCTTT AATCAGCCGA 4267 .......... .......... .......... .......... .......... .......... 1183 TACAAACTTG TACTCTTGCC GACTTCCCCT GTTGCGTTCA ACCGTTGTCA TAATTTACTT 4327 .......... .......... .......... .......... .......... .......... 1183 GTACCTTGAA TTATCTATTA ATAACTCAAC TCCCTTTGAT CCATCTGTTT GATACAGGTC 4387 ||| .......... .......... .......... .......... .......... .......GTC 1186 ATTTAACTAG CAAGAGTGAT GTGTATAGTT TTGGAGTTGT CCTCCTTGAA ATGCTGTCAG 4447 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTAACTAG CAAGAGTGAT GTGTATAGTT TTGGAGTTGT CCTCCTTGAA ATGCTGTCAG 1246 GTCGTAGGGC AATCGACAAG AATAGACCAT CCGGAGAACA CAATCTAGTG GAGTGGGCGA 4507 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCGTAGGGC AATCGACAAG AATAGACCAT CCGGAGAACA CAATCTAGTG GAGTGGGCGA 1306 AACCTTACCT AGGTAACAAA CGTAAGGTCT TCCGTGTTCT GGATACCCGT CTTGAAGGCC 4567 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACCTTACCT AGGTAACAAA CGTAAGGTCT TCCGTGTTCT GGATACCCGT CTTGAAGGCC 1366 AGTATTCAAT GGAGGTGGCG AGTAAAGTAG CTAACCTTGC CCTGCGGTGC CTATCCAAAG 4627 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATTCAAT GGAGGTGGCG AGTAAAGTAG CTAACCTTGC CCTGCGGTGC CTATCCAAAG 1426 ATCCTCGGTT TAGACCAAGC ATGAGTGACA TTGTTAAAGA ACTGGAGCAA CTTTATCAAC 4687 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCCTCGGTT TAGACCAAGC ATGAGTGACA TTGTTAAAGA ACTGGAGCAA CTTTATCAAC 1486 AATCTAAAGA TTCAGGAAAT ACTCGCAGCC ACGCTAGCAA CCGACCTAGA CCACGTAGAC 4747 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATCTAAAGA TTCAGGAAAT ACTCGCAGCC ACGCTAGCAA CCGACCTAGA CCACGTAGAC 1546 GAAGTGCTGG TGATGTTGCT AATAGAAATC CTTCAGTTGC TTATCCGAGA CCGTCTGCTT 4807 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGCTGG TGATGTTGCT AATAGAAATC CTTCAGTTGC TTATCCGAGA CCGTCTGCTT 1606 CTCCCCTTTA CGCTAAATAA TTAAACAAAT GATTGAAAGA TGCCTTAATT TCTTGTTTTT 4867 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCCCTTTA CGCTAAATAA TTAAACAAAT GATTGAAAGA TGCCTTAATT TCTTGTTTTT 1666 TTTGTCTTAT ATGTGTTTAT CAAGCTTGAT AAGTAGTTGA GGCTGATGAA GAACCTCTGC 4927 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGTCTTAT ATGTGTTTAT CAAGCTTGAT AAGTAGTTGA GGCTGATGAA GAACCTCTGC 1726 ACATTTGCAG TATCAGCTGG CTGATTGTAC AGTTGTTTTT GGCTAATGTA TTGAATCTAT 4987 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATTTGCAG TATCAGCTGG CTGATTGTAC AGTTGTTTTT GGCTAATGTA TTGAATCTAT 1786 ACTTTCTGTA GGTCCTGAAT GTGTTTGTAA CTTTGAGTTT TGCTCACAGC TCACTATATG 5047 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTTTCTGTA GGTCCTGAAT GTGTTTGTAA CTTTGAGTTT TGCTCACAGC TCACTATATG 1846 TTCACTTTTT GACACTAAAG GAATGCATAC TACCCTTTCA TACT 5091 |||||||||| |||||||||| |||||||||| |||||||||| |||| TTCACTTTTT GACACTAAAG GAATGCATAC TACCCTTTCA TACT 1890 hqPGS_C06HBa0016K14.1-4+_SGN-U319927+ (1808 2279,2658 2965,3354 3489,3575 3717,3818 3941,4385 5091) ******************************************************************************** EST sequence 8 +strand 731 n (File: SGN-U319928+) 1 CTTTCTTATC TTTTCCAAAA CCAAAACTAC TGACTTTTCT TGAAAAAAGA AGAGGTGGGG 61 TGCTTTCTTT TCTTCAAAAA CCTTCTCTTT GTTCTTGAAA AAACAGGACT CATTCATTTT 121 TTTTTGTGTG TTTCTTTCAG AAGAAATAAC AAAGACCCTT TCTCTGTTTT CTTCATATTT 181 CAGCTTTGAG CTACTTGGAT CTGTTTTTTT TTTTTTAAAA AAAAAAAAAT TTTGGGGGTT 241 CGGGGGCCTA CAAAAAAAGG AAAACCTAAA AGGGGGGATT TTGTTTTTTG TTTGTTGTTG 301 TTCAAAGGGG AAAATGTTTG AGGGCTAAAA TTAAACCTAA AACCCCTTTT CATACAGGGC 361 TAAATTCAAA ATCTGTTACC ATAAATGGGG GGGAAAAAAA TTCAAGCTCC AGGGTTCCTT 421 CAACTACTAA AAGTGAAGGA AAAATATTGC AGTCTCCCAA TTTAAAAACC TTTATTTTTT 481 CTAATCTCAA AACAGCCACT AGGAATTTTC TTCCCAATAG TGTGCTGGAA AAAGGGGAAT 541 TTGAATCATT TTTTAAAGGG GGAATCAATA AAAACACTTT CCCACCTACC AACCCTGGAA 601 CAGGTGTGAT CTTTGCTGTA AAAAGGCTTA ATCAAAAAGG TTTTCAAGGC CACAGGGAAT 661 GGTTGGCAAA ATTTAACTAT TTGGGGCAGT TTTTTCATCC CCATCTTGAA AAGCTAATTG 721 GCTACTGCTT G Predicted gene structure (within gDNA segment 1323 to 7898): Exon 1 1923 2279 ( 357 n); cDNA 1 357 ( 357 n); score: 0.927 Intron 1 2280 2657 ( 378 n); Pd: 0.992 (s: 0.84), Pa: 0.943 (s: 0.84) Exon 2 2658 2965 ( 308 n); cDNA 358 665 ( 308 n); score: 0.864 Intron 2 2966 3353 ( 388 n); Pd: 0.944 (s: 0.92), Pa: 0.981 (s: 0.90) Exon 3 3354 3419 ( 66 n); cDNA 666 731 ( 66 n); score: 0.909 MATCH C06HBa0016K14.1-4+ SGN-U319928+ 0.899 731 1.000 C PGS_C06HBa0016K14.1-4+_SGN-U319928+ (1923 2279,2658 2965,3354 3419) Alignment (genomic DNA sequence = upper lines): CTTTCTTATC TTTTCCAAAA CCAAAACTAC TGACTTTTCT TGAAAAAAGA AGAGGTGGGG 1982 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTTCTTATC TTTTCCAAAA CCAAAACTAC TGACTTTTCT TGAAAAAAGA AGAGGTGGGG 60 TGCTTTCTTT TCTTCAAAAA CCTTCTCTTT GTTCTTGAAA AAACAGGACT CATTCATTTT 2042 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTTTCTTT TCTTCAAAAA CCTTCTCTTT GTTCTTGAAA AAACAGGACT CATTCATTTT 120 TTTTTGTGTG TTTCTTTCAG AAGAAATAAC AAAGACCCTT TCTCTGTTTT CTTCATATTT 2102 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTGTGTG TTTCTTTCAG AAGAAATAAC AAAGACCCTT TCTCTGTTTT CTTCATATTT 180 CAGCTTTGAG CTACTTGGAT CTGTTTTTTT TTTTTTGAAT ATACAAGTAG TTTGTGTGTT 2162 |||||||||| |||||||||| |||||||||| |||||| || | | || | |||| | ||| CAGCTTTGAG CTACTTGGAT CTGTTTTTTT TTTTTTAAAA AAAAAAAAAT TTTGGGGGTT 240 CTGGGGTCTA CAGAAGAAGG AGAAGCTAAA AGGGGTGATT TTGTTTTTTG TTTGTTGTTG 2222 | |||| ||| || || |||| | || ||||| ||||| |||| |||||||||| |||||||||| CGGGGGCCTA CAAAAAAAGG AAAACCTAAA AGGGGGGATT TTGTTTTTTG TTTGTTGTTG 300 TTCTAATGGG GATATGTTTG AGTGCTAGAA TTAAAGCTGA AAGCCCATTT CATACAGGTA 2282 ||| || ||| | ||||||| || |||| || ||||| || | || ||| ||| ||||||| TTCAAAGGGG AAAATGTTTG AGGGCTAAAA TTAAACCTAA AACCCCTTTT CATACAG... 357 TTTGATATAA GACTATGAAC TCTTTTTTTT CTTCTTGTTT ACTTGCACAA TTTTTGCAAA 2342 .......... .......... .......... .......... .......... .......... 357 TTTGAGTCTT TTTGGAGGAG GGGTTCTGTA GAATGGTTTT GTTTGTTTGT TTTCTAATTT 2402 .......... .......... .......... .......... .......... .......... 357 TCATTCATAT TAGGATGATC ATGCCTTTGT TCATGTAAAG TGGCTAGTTT GAGTTCTTGC 2462 .......... .......... .......... .......... .......... .......... 357 TAATTTTTTA GTAGTCTCTA TTTGGTAATA GTAGTTATCC TGATTAATAT ATCTTGTTGA 2522 .......... .......... .......... .......... .......... .......... 357 TAAAAAAAAA TTGGGGGTAG GGGTAGCAAA GGGGATTATA AGTTAGTTAA TTGAGCTACT 2582 .......... .......... .......... .......... .......... .......... 357 AAGATTTAAG ATGTTTTGTT GTGTTCTAAT GGGGTTAATT GATGGTAATC TAATAAATTG 2642 .......... .......... .......... .......... .......... .......... 357 AGAGAATTAT TGCAGGGCTA AATTCAAGAT CTGTTAGCAT AGATGGTGGT GATAGTAATT 2702 ||||| ||||||| || |||||| ||| | |||| || || | |||| .......... .....GGCTA AATTCAAAAT CTGTTACCAT AAATGGGGGG GAAAAAAATT 402 CAAGCTCCAG GGTTCCATCA ACTACTAGAA GTGAAGGAGA GATATTGCAG TCTCCCAATT 2762 |||||||||| |||||| ||| ||||||| || |||||||| | ||||||||| |||||||||| CAAGCTCCAG GGTTCCTTCA ACTACTAAAA GTGAAGGAAA AATATTGCAG TCTCCCAATT 462 TGAAGAGCTT TAGTTTTTCT GATCTCAGAA CAGCCACTAG GAACTTTCGT CCCGATAGTG 2822 | || | ||| || ||||||| |||||| || |||||||||| ||| |||| | ||| |||||| TAAAAACCTT TATTTTTTCT AATCTCAAAA CAGCCACTAG GAATTTTCTT CCCAATAGTG 522 TGCTGGGAGA AGGTGGATTT GGATCAGTTT TTAAAGGGTG GATCGATGAG AACACGTTCG 2882 |||||| | | ||| | |||| | |||| ||| |||||||| | ||| || | ||||| ||| TGCTGGAAAA AGGGGAATTT GAATCATTTT TTAAAGGGGG AATCAATAAA AACACTTTCC 582 CAGCTACCAA GCCTGGGACA GGTGTGATCA TTGCTGTGAA AAGGCTTAAT CAAGAAGGTT 2942 || ||||||| ||||| ||| ||||||||| ||||||| || |||||||||| ||| |||||| CACCTACCAA CCCTGGAACA GGTGTGATCT TTGCTGTAAA AAGGCTTAAT CAAAAAGGTT 642 TTCAAGGTCA CAGGGAATGG CTGGTGAGTT TTTTTTTTTT ATGTTTGATA TGGAGATGTT 3002 ||||||| || |||||||||| || TTCAAGGCCA CAGGGAATGG TTG....... .......... .......... .......... 665 CGAGTCGGTT TAAGCTTAAA GTGCATTGAT TATACCATGA ACTCAGAGTA AGCGGAACTA 3062 .......... .......... .......... .......... .......... .......... 665 GATCATTTGC TATTGGTTTG GACGAAGCCA GTAGCTTTTG CTTAGTCCCT GTATTTGTAT 3122 .......... .......... .......... .......... .......... .......... 665 TAGGAAATTC ATTAAATATG TATGAAACGT TTTATTGCGA AACCTACTAA AGAAGATGAA 3182 .......... .......... .......... .......... .......... .......... 665 TTATGAGATT TGAGGCAAGT TCAGAACCCA TAAACTTCAA ATCTTGATTC TGCCTCTACA 3242 .......... .......... .......... .......... .......... .......... 665 AGAATTGTCG TTGTTACTTA TTGAGTGCTT TATTAAGTGC TAATACTTTG TTATTTTGAG 3302 .......... .......... .......... .......... .......... .......... 665 TGTCATAAGG TTGTCTGAGT TCTGATTTGT ATTATGCTTC TATGTTTTTA GGCAGAAGTT 3362 ||| || || .......... .......... .......... .......... .......... .GCAAAATTT 674 AACTATTTGG GGCAGTTTTC TCATCCTCAT CTTGTAAAGC TGATTGGCTA CTGCTTG 3419 |||||||||| ||||||||| |||||| ||| |||| ||||| | |||||||| ||||||| AACTATTTGG GGCAGTTTTT TCATCCCCAT CTTGAAAAGC TAATTGGCTA CTGCTTG 731 hqPGS_C06HBa0016K14.1-4+_SGN-U319928+ (1923 2279,2658 2965,3354 3419) ******************************************************************************** EST sequence 2 +strand 833 n (File: SGN-U336971+) 1 ATGAAAGCGG CCCCGCGCCC CGNCCAAATG ATGACATCTA TGAGACTCAT GCTGGAGCTC 61 CACCGGGTGG CGGACGCTCT AGAACTAGTG GATCCCCCGG GCTGCAGGCC AAAACCAAAA 121 CTACTGACTT TTCTTGAAAA AAGAAGAGGT GGGGTGCTTT CTTTTCTTCA AAAACCTTCT 181 CTTTGTTCTT GAAAGAACAG GACTCATTCA TTTTTTTTGT GTGTTTCTTT CAGAAGAAAT 241 AACAAAGACC CTTTCTCTGT TAACTTCATA TTTCAGCTTT GAGCTACTTG GATCTGTTTT 301 TTTTTTTTTT GAATATACAA CTACATCGTG TGTTCTGGGG TCTACAGAAA AAGGAGAATC 361 TCAAAGGGGT GATTGTGATT TTTGATTGTT GTTGCTCTAA TGGGGATATG TCTGAGTGCT 421 ATAATTAAAG CTGAAAGCCC ATTTCATACA CGGCTAAATT CAACATCTGT TGCATAAATG 481 GCGGCGATAG TAATTCAAGC TCCTGGGTTC CATCCACTAC TAGAAGCGAA CGAGAGATGA 541 TGCTGACTCT CCATTTGATG AGCTTTAGTC TATCTGATCT CAGACACTCA CTACGAACTA 601 TCTTTCCGTA GTGTGCTGTG AGACGGCGGA TTGGATCACT TATAGAGGGT GGATCCATGA 661 GACACGACGC CCCTACTCGG CTTGTGCCAG GTGGATCATT GTATGACAAG TTAATCAAGA 721 CGCNTTAACG CGACAGGAAT GCTGGCAGAC TTACCTATTG TGCAGGTTTC ATACTCTCTT 781 GTAACTGATG GATCTGCTGG AGGACAAATC NCTTTGCTAT GAGTATCCTT GGT Predicted gene structure (within gDNA segment 257 to 6718): Exon 1 1937 2279 ( 343 n); cDNA 109 451 ( 343 n); score: 0.940 Intron 1 2280 2657 ( 378 n); Pd: 0.992 (s: 0.94), Pa: 0.943 (s: 0.90) Exon 2 2658 2965 ( 308 n); cDNA 452 744 ( 293 n); score: 0.799 Intron 2 2966 3353 ( 388 n); Pd: 0.944 (s: 0.70), Pa: 0.981 (s: 0) Exon 3 3354 3358 ( 5 n); cDNA 745 749 ( 5 n); score: 1.000 MATCH C06HBa0016K14.1-4+ SGN-U336971+ 0.873 656 0.788 C PGS_C06HBa0016K14.1-4+_SGN-U336971+ (1937 2279,2658 2965,3354 3358) Alignment (genomic DNA sequence = upper lines): CCAAAACCAA AACTACTGAC TTTTCTTGAA AAAAGAAGAG GTGGGGTGCT TTCTTTTCTT 1996 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCAAAACCAA AACTACTGAC TTTTCTTGAA AAAAGAAGAG GTGGGGTGCT TTCTTTTCTT 168 CAAAAACCTT CTCTTTGTTC TTGAAAAAAC AGGACTCATT CATTTTTTTT TGTGTGTTTC 2056 |||||||||| |||||||||| |||||| ||| |||||||||| || ||||||| |||||||||| CAAAAACCTT CTCTTTGTTC TTGAAAGAAC AGGACTCATT CA-TTTTTTT TGTGTGTTTC 227 TTTCAGAAGA AATAACAAAG ACCCTTTCTC TGTTTTCTTC ATATTTCAGC TTTGAGCTAC 2116 |||||||||| |||||||||| |||||||||| |||| |||| |||||||||| |||||||||| TTTCAGAAGA AATAACAAAG ACCCTTTCTC TGTTAACTTC ATATTTCAGC TTTGAGCTAC 287 TTGGATCTG- TTTTTTTTTT TTTGAATATA CAAGTAGTTT GTGTGTTCTG GGGTCTACAG 2175 ||||||||| |||||||||| |||||||||| ||| || | |||||||||| |||||||||| TTGGATCTGT TTTTTTTTTT TTTGAATATA CAACTACATC GTGTGTTCTG GGGTCTACAG 347 AAGAAGGAGA AGCTAAAAGG GGTGATTTTG TTTTTTGTTT GTTGTTGTTC TAATGGGGAT 2235 || ||||||| | || ||||| ||||||| || |||||| || ||||||| || |||||||||| AAAAAGGAGA ATCTCAAAGG GGTGATTGTG ATTTTTGATT GTTGTTGCTC TAATGGGGAT 407 ATGTTTGAGT GCTAGAATTA AAGCTGAAAG CCCATTTCAT ACAGGTATTT GATATAAGAC 2295 |||| ||||| |||| ||||| |||||||||| |||||||||| ||| ATGTCTGAGT GCTATAATTA AAGCTGAAAG CCCATTTCAT ACAC...... .......... 451 TATGAACTCT TTTTTTTCTT CTTGTTTACT TGCACAATTT TTGCAAATTT GAGTCTTTTT 2355 .......... .......... .......... .......... .......... .......... 451 GGAGGAGGGG TTCTGTAGAA TGGTTTTGTT TGTTTGTTTT CTAATTTTCA TTCATATTAG 2415 .......... .......... .......... .......... .......... .......... 451 GATGATCATG CCTTTGTTCA TGTAAAGTGG CTAGTTTGAG TTCTTGCTAA TTTTTTAGTA 2475 .......... .......... .......... .......... .......... .......... 451 GTCTCTATTT GGTAATAGTA GTTATCCTGA TTAATATATC TTGTTGATAA AAAAAAATTG 2535 .......... .......... .......... .......... .......... .......... 451 GGGGTAGGGG TAGCAAAGGG GATTATAAGT TAGTTAATTG AGCTACTAAG ATTTAAGATG 2595 .......... .......... .......... .......... .......... .......... 451 TTTTGTTGTG TTCTAATGGG GTTAATTGAT GGTAATCTAA TAAATTGAGA GAATTATTGC 2655 .......... .......... .......... .......... .......... .......... 451 AGGGCTAAAT TCAAGATCTG TTAGCATAGA TGGTGGTGAT AGTAATTCAA GCTCCAGGGT 2715 |||||||| |||| ||||| || ||||| | ||| || ||| |||||||||| ||||| |||| ..GGCTAAAT TCAACATCTG TT-GCATAAA TGGCGGCGAT AGTAATTCAA GCTCCTGGGT 508 TCCATCAACT ACTAGAAGTG AAGGAGAGAT ATTGCAGTCT CCCAATTTGA AGAGCTTTAG 2775 |||||| ||| |||||||| | || ||||||| ||| | || | | |||||| ||||||||| TCCATCCACT ACTAGAAGCG AACGAGAGAT GATGCTGACT CTCCATTTGA TGAGCTTTAG 568 TTTTTCTGAT CTCAGAACAG CCACTAGGAA CTTTCGTCCC GATAGTGTGC TGGGAGAAGG 2835 | | |||||| ||||| ||| ||||| ||| || || | || | |||||||| || |||| || TCTATCTGAT CTCAG-ACAC TCACTACGAA CTATCTTTCC G-TAGTGTGC TGTGAGACGG 626 TGGATTTGGA TCAGTTTTTA AAGGGTGGAT CGATGAGAAC ACGTTCGCAG CTACCAAGCC 2895 ||| ||||| ||| || || ||||||||| | ||||| || ||| ||| |||| || CGGA-TTGGA TCA-CTTATA GAGGGTGGAT CCATGAG-AC ACG-ACGCCC CTACTCGGCT 682 TGGGACAGGT GTGATCATTG CTGTGAAAAG GCTTAATCAA GAAGGTTTTC AAGGTCACAG 2955 || | ||||| | |||||||| | ||| || | |||||||| | | | || || | ||| TGTGCCAGGT G-GATCATTG -TATGACAA- G-TTAATCAA G-ACGCNTT- AACGCGACA- 735 GGAATGGCTG GTGAGTTTTT TTTTTTTATG TTTGATATGG AGATGTTCGA GTCGGTTTAA 3015 ||||| |||| GGAAT-GCTG .......... .......... .......... .......... .......... 744 GCTTAAAGTG CATTGATTAT ACCATGAACT CAGAGTAAGC GGAACTAGAT CATTTGCTAT 3075 .......... .......... .......... .......... .......... .......... 744 TGGTTTGGAC GAAGCCAGTA GCTTTTGCTT AGTCCCTGTA TTTGTATTAG GAAATTCATT 3135 .......... .......... .......... .......... .......... .......... 744 AAATATGTAT GAAACGTTTT ATTGCGAAAC CTACTAAAGA AGATGAATTA TGAGATTTGA 3195 .......... .......... .......... .......... .......... .......... 744 GGCAAGTTCA GAACCCATAA ACTTCAAATC TTGATTCTGC CTCTACAAGA ATTGTCGTTG 3255 .......... .......... .......... .......... .......... .......... 744 TTACTTATTG AGTGCTTTAT TAAGTGCTAA TACTTTGTTA TTTTGAGTGT CATAAGGTTG 3315 .......... .......... .......... .......... .......... .......... 744 TCTGAGTTCT GATTTGTATT ATGCTTCTAT GTTTTTAGGC AGA 3358 || ||| .......... .......... .......... ........GC AGA 749 hqPGS_C06HBa0016K14.1-4+_SGN-U336971+ (1937 2279,2658 2965) ******************************************************************************** EST sequence 3 +strand 1290 n (File: SGN-U336972+) 1 TGTGAACAAC GGGACTCCCC GNGGCGGCCG TNCTAAAACT TTGGTNCCCC CGGGCTGCAG 61 GAATTCGGCA CGAGGCTGAC TTTTCTTGAA AAAAGAAGAG GTGGGGTGCT TTCTTTTCTT 121 CAAAAACCTT CTCTTTGTTC TTGAAAAAAC AGGACTCATT CATTTTTTTT TGTGTGTTTC 181 TTTCAGAAGA AATAACAAAG ACCCTTTCTC TGTTTTCTTC ATATTTCAGC TTTGAGCTAC 241 TTGGATCTGT TTTTTTTTTT TTGGAATATA CAATTATTTG GGGGGTCCGG GGGTTTACAA 301 AAAAAGGAAA ACCTAAAGGG GGGGATTTGG TTTTTTGTTT GTGGGGGTTC TAAGGGGGAT 361 ATGTTGGAGG CCCAAAATTA AACCTGAAGC CCCTTTTTTA CGGGGCAAAA TAAAAAATTT 421 GTAACCACCA CCGGGGGGGG AGTTATTTTT TTCTCCGGGT TCCTTTATTT ATTAGAAGTG 481 AAGGAGAGAT TTCCAAAACC CCCAAAAAAA AACCTTTGTT TCTTTGGAGG GGGACCCCCC 541 CCCCCAAATT TTGTCCCCAG AGGGGCTTTT ATAGGGGTTC CCCACCCCCT TCCAGGGGTG 601 GGGGGGAAAA CCCCCAATTT TCCCCCCCCT TGGGCAGGAA AAAAACCCCC CCATAATTTG 661 GGAAGAAAAT TTTATGGCCC CCCACCCTGT GGAAAAAGGT TTTTTTGGCG GGGGGCCTCC 721 CCCGTTATTA AAAAAAAAAA AAATGGGCTG GGGGGGCAAA AAAAACCCAA ATTTTGGGGT 781 TGGAAGCCCC CCCAAACCCC TTTTTCAAAC ACCTGGGGAA AGAAAAGCTT TTTTTCTCCC 841 TTTAAAAAAA AACACCCCGG TTTTGTNAAA AAAAGGGGGG GGAAAACTCT TTTTTTCCAA 901 AGAGTGTTGG ACCCCCCGGG GCTTGAGTAA TTAACAAAAA AAATTATGTG TTTTTTTCCC 961 CAAACGCCCC CTTTTTTTTA TAAAAGAAAA AACGGGGCCC GCCCCAAGAA GTCCCCCCCC 1021 CCCCCCCCCC CGGGGGAGGG GTTAATTATT TTTTTCCCCC CTTAAAACAG TTTCCCCCCC 1081 TTTAAAGGGG AGGAGGGTGG AAAATTCTCC CTATACCCCC ATAAAAACCG TTCCCCCCCC 1141 GCTCCCCAAA AAATCCCACA AGGTTTTTTT TGGTGGGGAA AAAACAAAAC CGGGGGGAAG 1201 GGGGGTTCTT CTTCTTTTTC TCCCCGGGGG GGGGCCCCCG GGAGGATACA CCCCCATCTC 1261 CCCCCGCGGG GTATCCCATA TGGGGCCCCT Predicted gene structure (within gDNA segment 602 to 13028): Exon 1 1953 2279 ( 327 n); cDNA 77 403 ( 327 n); score: 0.901 Intron 1 2280 2657 ( 378 n); Pd: 0.992 (s: 0.78), Pa: 0.943 (s: 0.54) Exon 2 2658 2745 ( 88 n); cDNA 404 490 ( 87 n); score: 0.670 MATCH C06HBa0016K14.1-4+ SGN-U336972+ 0.852 415 0.322 C PGS_C06HBa0016K14.1-4+_SGN-U336972+ (1953 2279,2658 2745) Alignment (genomic DNA sequence = upper lines): TGACTTTTCT TGAAAAAAGA AGAGGTGGGG TGCTTTCTTT TCTTCAAAAA CCTTCTCTTT 2012 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGACTTTTCT TGAAAAAAGA AGAGGTGGGG TGCTTTCTTT TCTTCAAAAA CCTTCTCTTT 136 GTTCTTGAAA AAACAGGACT CATTCATTTT TTTTTGTGTG TTTCTTTCAG AAGAAATAAC 2072 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTCTTGAAA AAACAGGACT CATTCATTTT TTTTTGTGTG TTTCTTTCAG AAGAAATAAC 196 AAAGACCCTT TCTCTGTTTT CTTCATATTT CAGCTTTGAG CTACTTGGAT CTGTTTTTTT 2132 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAGACCCTT TCTCTGTTTT CTTCATATTT CAGCTTTGAG CTACTTGGAT CTGTTTTTTT 256 TTTTTT-GAA TATACAAGTA GTTTGTGTGT TCTGGGGTCT ACAGAAGAAG GAGAAGCTAA 2191 |||||| ||| ||||||| || || | | || | ||||| | ||| || ||| || || |||| TTTTTTGGAA TATACAATTA TTTGGGGGGT CCGGGGGTTT ACAAAAAAAG GAAAACCTAA 316 AAGGGGTGAT TTTGTTTTTT GTTTGTTGTT GTTCTAATGG GGATATGTTT GAGTGCTAGA 2251 | |||| ||| || ||||||| |||||| | ||||||| || ||||||||| ||| | | | AGGGGGGGAT TTGGTTTTTT GTTTGTGGGG GTTCTAAGGG GGATATGTTG GAGGCCCAAA 376 ATTAAAGCTG AAAGCCCATT TCATACAGGT ATTTGATATA AGACTATGAA CTCTTTTTTT 2311 |||||| ||| |||||| || | ||| | ATTAAACCTG -AAGCCCCTT TTTTACGG.. .......... .......... .......... 403 TCTTCTTGTT TACTTGCACA ATTTTTGCAA ATTTGAGTCT TTTTGGAGGA GGGGTTCTGT 2371 .......... .......... .......... .......... .......... .......... 403 AGAATGGTTT TGTTTGTTTG TTTTCTAATT TTCATTCATA TTAGGATGAT CATGCCTTTG 2431 .......... .......... .......... .......... .......... .......... 403 TTCATGTAAA GTGGCTAGTT TGAGTTCTTG CTAATTTTTT AGTAGTCTCT ATTTGGTAAT 2491 .......... .......... .......... .......... .......... .......... 403 AGTAGTTATC CTGATTAATA TATCTTGTTG ATAAAAAAAA ATTGGGGGTA GGGGTAGCAA 2551 .......... .......... .......... .......... .......... .......... 403 AGGGGATTAT AAGTTAGTTA ATTGAGCTAC TAAGATTTAA GATGTTTTGT TGTGTTCTAA 2611 .......... .......... .......... .......... .......... .......... 403 TGGGGTTAAT TGATGGTAAT CTAATAAATT GAGAGAATTA TTGCAGGGCT AAATTCAAGA 2671 ||| |||| || | .......... .......... .......... .......... ......GGCA AAATAAAAAA 417 TCTGTTAGCA TAGATGGTGG TGATAGTAAT TCAAGCTCCA GGGTTCCATC AACTACTAGA 2731 | ||| | || || || | || | | |||| ||||||| | | || |||| TTTGTAACCA CCACCGGGGG GGGAGTTATT TTTTTCTCC- GGGTTCCTTT ATTTATTAGA 476 AGTGAAGGAG AGAT 2745 |||||||||| |||| AGTGAAGGAG AGAT 490 hqPGS_C06HBa0016K14.1-4+_SGN-U336972+ (1953 2279) ******************************************************************************** EST sequence 12 -strand 854 n (File: SGN-U339313-) 1 CCTTGTGGAC TATTGAGGCA AGGGATTTTC TAATTCTTCT AAGTGTATGA ATGCGTGTAT 61 GTCCTTGTGT TATATGTTGG AGTACGGGAT GGGGTATGGG TTGACTTGTC CACATTGATT 121 ANTTCTAATG ATGAAAGAAA GGGGATACAA AAGGCAATGT GATTAATGGT TTATGTGTGT 181 TGTGTTGAGA AAGAATCGAA GGCTTGTTGA ATCATTGTTG ATGTAACTTG ATGTTTGTGT 241 GGTTGTGAAC TGTAGGATGT TATGAAAATG GTCATCTCCT CATTATATGT GTGAACTTGT 301 CATCTGCATT ATTCTGAGGC ATTGGTTGTG GCAAGTGTTA TGTGCATTGA GAAATAATGG 361 GTACTTAAGA GGATGTACCA TTTCGAGGGA CGTATCGCGC GCCGCGATGG ATACTATATT 421 TCGAGGAACG TATCGCGCGC CGTGATGGTT ACTATTATCG AGGATCGTGT CGTGCGCCGC 481 GACAGATGCA TGGACAGATA TGTCCCCCAA GGGTCCCGGA CTGAGAGACA GCGGGTGTAT 541 ATCACTAGGT CAGACATGCA TCATTATACT TGACATTGCA TTCCATTGCA TTACACACTT 601 ATCATTAGTG AACTTGATAT CGTATTTTCT CGATCTTCTT ATTGCCTTTC TGCAGAACTT 661 GTGATTGATT AATATTGAGC TTGTTATGGC GGATATATAA TTTGTTGAAC TATTCTTGTT 721 GAGGATATGT AATTTGTTGA AGTGTTGTTG TTGAGGATAT GTAATTTGTC CTCGTGCCGA 781 ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGACC GCCACCGCGG TGGAGCTCCA 841 GCATTGGTTT CAAT Predicted gene structure (within gDNA segment 2514 to 11597): Exon 1 3932 3941 ( 10 n); cDNA 357 366 ( 10 n); score: 0.800 Intron 1 3942 7663 (3722 n); Pd: 0.999 (s: 0), Pa: 0.502 (s: 0.86) Exon 2 7664 8008 ( 345 n); cDNA 367 708 ( 342 n); score: 0.814 Intron 2 8009 10521 (2513 n); Pd: 0.000 (s: 0.70), Pa: 0.413 (s: 0.63) Exon 3 10522 10562 ( 41 n); cDNA 709 747 ( 39 n); score: 0.634 MATCH C06HBa0016K14.1-4+ SGN-U339313- 0.814 396 0.464 C PGS_C06HBa0016K14.1-4+_SGN-U339313- (3932 3941,7664 8008,10522 10562) Alignment (genomic DNA sequence = upper lines): ATGGCTACTG GTATGGTTTC TTTTCCCCCC TGACTCATGT GTTAGTTTTC TCTCTTGTTC 3991 |||| |||| ATGGGTACTT .......... .......... .......... .......... .......... 366 CAACGTGGCC CGACATCCAT AGAGTATCCA AATTTAGAAT ATTAGGTACT GATCAGGACA 4051 .......... .......... .......... .......... .......... .......... 366 TCTTTACGTC TAAGATGGAA CATTTTTTCT TATCCAAGAT CCATCTAACA TGCAGTTTTC 4111 .......... .......... .......... .......... .......... .......... 366 TCTTTTCTTT TTAACTCGCA TTCTTATTGT CAAGAACCTC ATTAGTCATT CACCTATGAT 4171 .......... .......... .......... .......... .......... .......... 366 GCATTTGTAC TGCACTCCAT CCCCTGGGCA ACAATTAGTT ACTGCTCTTT CTGGGATGGA 4231 .......... .......... .......... .......... .......... .......... 366 CTTTGATATC TTCATGTTGA GTCTTTAATC AGCCGATACA AACTTGTACT CTTGCCGACT 4291 .......... .......... .......... .......... .......... .......... 366 TCCCCTGTTG CGTTCAACCG TTGTCATAAT TTACTTGTAC CTTGAATTAT CTATTAATAA 4351 .......... .......... .......... .......... .......... .......... 366 CTCAACTCCC TTTGATCCAT CTGTTTGATA CAGGTCATTT AACTAGCAAG AGTGATGTGT 4411 .......... .......... .......... .......... .......... .......... 366 ATAGTTTTGG AGTTGTCCTC CTTGAAATGC TGTCAGGTCG TAGGGCAATC GACAAGAATA 4471 .......... .......... .......... .......... .......... .......... 366 GACCATCCGG AGAACACAAT CTAGTGGAGT GGGCGAAACC TTACCTAGGT AACAAACGTA 4531 .......... .......... .......... .......... .......... .......... 366 AGGTCTTCCG TGTTCTGGAT ACCCGTCTTG AAGGCCAGTA TTCAATGGAG GTGGCGAGTA 4591 .......... .......... .......... .......... .......... .......... 366 AAGTAGCTAA CCTTGCCCTG CGGTGCCTAT CCAAAGATCC TCGGTTTAGA CCAAGCATGA 4651 .......... .......... .......... .......... .......... .......... 366 GTGACATTGT TAAAGAACTG GAGCAACTTT ATCAACAATC TAAAGATTCA GGAAATACTC 4711 .......... .......... .......... .......... .......... .......... 366 GCAGCCACGC TAGCAACCGA CCTAGACCAC GTAGACGAAG TGCTGGTGAT GTTGCTAATA 4771 .......... .......... .......... .......... .......... .......... 366 GAAATCCTTC AGTTGCTTAT CCGAGACCGT CTGCTTCTCC CCTTTACGCT AAATAATTAA 4831 .......... .......... .......... .......... .......... .......... 366 ACAAATGATT GAAAGATGCC TTAATTTCTT GTTTTTTTTG TCTTATATGT GTTTATCAAG 4891 .......... .......... .......... .......... .......... .......... 366 CTTGATAAGT AGTTGAGGCT GATGAAGAAC CTCTGCACAT TTGCAGTATC AGCTGGCTGA 4951 .......... .......... .......... .......... .......... .......... 366 TTGTACAGTT GTTTTTGGCT AATGTATTGA ATCTATACTT TCTGTAGGTC CTGAATGTGT 5011 .......... .......... .......... .......... .......... .......... 366 TTGTAACTTT GAGTTTTGCT CACAGCTCAC TATATGTTCA CTTTTTGACA CTAAAGGAAT 5071 .......... .......... .......... .......... .......... .......... 366 GCATACTACC CTTTCATACT TAGTAGTTTC TTGGTCTTCG ATTTTTGTTA TCATCTCTTG 5131 .......... .......... .......... .......... .......... .......... 366 TTTCTTGTAC TTAATTTTGT AGTAATAATT TTTTTAGAAT GCTTTATAAT CTTATCTTTA 5191 .......... .......... .......... .......... .......... .......... 366 GTATTTTTAT CTTGGCCTTT TTACCTTCAT ATACCTTTTC TTGAGTTGTA GGGTCTATCG 5251 .......... .......... .......... .......... .......... .......... 366 AAAACTACCT CTTTTATCTT CAAGGCGGGA GTAACTTTGC TTACAAACTA CCCTTCTTTA 5311 .......... .......... .......... .......... .......... .......... 366 ACCTTACTTG TGAAATTATA CTAGGTATGT TATTATTACG AGGGAGTGCA AAGTGCTATT 5371 .......... .......... .......... .......... .......... .......... 366 TGCATAATTG ATGTTGGCAA CCTTTTCACC CTATGAAAAG TTAAAACTTT TTAAGTCAAA 5431 .......... .......... .......... .......... .......... .......... 366 AGTACTTGTG TTATGTCTAT TTCAGCTTTT CTATATGTTA CATCCCCTTT TTAGAATCTG 5491 .......... .......... .......... .......... .......... .......... 366 TGGGATACAT TGGGCTTTTC TTTTTATCCT TTAGTGTAAA GTATATAATA GCAAGATGGG 5551 .......... .......... .......... .......... .......... .......... 366 AAGATTCAAA AAAAGTTGGT AGTGGAAAAA ATAATGTTAT CCATAAGAGT GATGTAAAGA 5611 .......... .......... .......... .......... .......... .......... 366 GAAGTAGTCA CAATAGGATT GTAGAAAGAT GCAAATCCAT TTCTTCTTTC CAATATTATA 5671 .......... .......... .......... .......... .......... .......... 366 GGCCATTTGA GGGGTCCTCC AAAAAAAATA AAATATCATT AGTGCTATAT TCTAAAGTGG 5731 .......... .......... .......... .......... .......... .......... 366 CCATATCTTA GGTATCTTTT GAATATCAAA TTGTTGGAGA ATGACACAAA GTGGTGAAGC 5791 .......... .......... .......... .......... .......... .......... 366 TTAAGACAAA AATAAGGATA ATGTTATCAA GGATATTTTG TCGATAGTTG TTACTGACGT 5851 .......... .......... .......... .......... .......... .......... 366 ATTTTTCAAG ATTCAACAAG CTCAAGATGA AACAAAGAGT TTCAATATTT ATTTATTTTT 5911 .......... .......... .......... .......... .......... .......... 366 ATAAAGGAAC AAGGAGTTTC GTCTTTTTTT CTTTTTAACA AATGAACAAA GAAGGTTAGC 5971 .......... .......... .......... .......... .......... .......... 366 CCGTTTTAAC CGTTCAATTA TTTTTATTTT GGAGAACTAT TCATTTTTAT GTTTTTTGAG 6031 .......... .......... .......... .......... .......... .......... 366 TCATCGGAGT CTGTCTCAAA TGATTATTTA ATTCTTTATA ATTGAGGATT TGGAGATATT 6091 .......... .......... .......... .......... .......... .......... 366 TGTCATATAT ATTATTCATG AATAATATAT AAATATTTTG TTAAATTTTA AGTCTTAAAG 6151 .......... .......... .......... .......... .......... .......... 366 TTTATCATTT TCTCAAGTCC AACATGTAAA TTTTAGTTCA TCTATTATAA TCATAAGTCC 6211 .......... .......... .......... .......... .......... .......... 366 AAGTCCATCA AAATTCATTA CTTTAGTCTT TACTTATAAG ATAGTTACAC CTTCTATTAA 6271 .......... .......... .......... .......... .......... .......... 366 TTGAATCAAA CAAAGAGTTT CAATATTTAT TTATTTTTAC AAAAGAACAA AGAATTTTGT 6331 .......... .......... .......... .......... .......... .......... 366 CTTTTTTTCT TTTAACAAAT GAACAAAGAA GGTTAGCCCA TTTTGACCGT TCAATTATTT 6391 .......... .......... .......... .......... .......... .......... 366 TTATTTTGGA GAACTATTTA TTTTTATGTT TTTTGAGTGA TCGGAGTCTG TCTTAAATGA 6451 .......... .......... .......... .......... .......... .......... 366 TTATTTAATT CTTTATATTT GAGGATTTGG AGATATTAGT CATATATATT ATTCATAAAT 6511 .......... .......... .......... .......... .......... .......... 366 AATATATAAA TATTTTTGTA ATGACCCGGA AGGTCATTTT TGGAAATTTT AAATATTAGT 6571 .......... .......... .......... .......... .......... .......... 366 GTAACTTGAG TTAATTAATC GATAGTTTAT GGGCTAAAAT GATAATTTAT TTAAGACCCT 6631 .......... .......... .......... .......... .......... .......... 366 ATACTATTTA GCCTTTATTT AATTAAATAT GTGGAACAAA ACTAACCTAA ACGTGTGACG 6691 .......... .......... .......... .......... .......... .......... 366 AAAAACAAAG AAGAGGAGAA AGTCTGAAAA ACACTCGCAG CGCCGTCGAA CAAGTGCACT 6751 .......... .......... .......... .......... .......... .......... 366 TGATTCAGGT AATAATTTTA AACTTTGCAT TGAGGCTGCT GAATATGTCT TGTAATTAAT 6811 .......... .......... .......... .......... .......... .......... 366 GTACAATTAT AATTTTTATG TTAACTTTAA TATGTGCTTT GGAATGTGAA ACATGGCAAA 6871 .......... .......... .......... .......... .......... .......... 366 ATGAATATGG AATAAAGAAA TAATATTTGG AAGTTGGTAT ATTGGTATGA AGTAATTTTG 6931 .......... .......... .......... .......... .......... .......... 366 AAATTTAGAA AGAGGGTCAT GCCAATCATG TGATAATTGT TGGGAATATA ATTGCATCAA 6991 .......... .......... .......... .......... .......... .......... 366 GATGATTGAA AAATAATAGA TAGGAATATA GGGTATCCAT TTGAGGATTT AAAATATATT 7051 .......... .......... .......... .......... .......... .......... 366 TTTTTGTCCT CGTGATTTTC TCTGTAACTT AAGGCTTCAC TGTTTCTTAG AATTTTCTTA 7111 .......... .......... .......... .......... .......... .......... 366 TTATTATCAT ATTAAGATTC AAGTGTGATA TATTGAGATA GGTAATTAAA TATTAGATAT 7171 .......... .......... .......... .......... .......... .......... 366 ATTATATTAT ATAGATATCG TTAAAGTTAT GATATTTGGA GAAATTGAAA TTTAACCGTA 7231 .......... .......... .......... .......... .......... .......... 366 AGCTTAAATT TGGAAAACAT GAGAGTTCAC ATGTTAATTG ACATCAAGAA TTACAAACTT 7291 .......... .......... .......... .......... .......... .......... 366 GATTATAAAT TTGGGTGAAG TTTTATGTCA AGGTTAAACA CATAATAATG TGGGTGTTGT 7351 .......... .......... .......... .......... .......... .......... 366 TAGTTCCAAA CCTTATTGTG AATATACATA TTTGAATAGA TTGCATCGAT TTGGAAGCTC 7411 .......... .......... .......... .......... .......... .......... 366 AACTTAAAGG GAAGGCTCAA GTCCCGTAGT GATTATTCAA GTGACTAAGG CAAGTGGATT 7471 .......... .......... .......... .......... .......... .......... 366 TCTAAACTCT TGTTAAGCGT ACGAAATTCG TGTATTTCCT TGTGTGATGT TGAGAATGAT 7531 .......... .......... .......... .......... .......... .......... 366 TTGGAGACTT GTTGTTTATT TGTTGGTGTT GTATTTCTTG TTGTAAATTG TGTTTGGTTA 7591 .......... .......... .......... .......... .......... .......... 366 TCAAATGGTT ATCTCCTCCC TTTTCATTTG AGCATGTCAT TTGCATTGTA TCTGAGACAT 7651 .......... .......... .......... .......... .......... .......... 366 TGTTGTGGTA AGAGGATGGT GTACTATTTT CGAGGGTCGT ATCGCGCGCC GCGATGGATA 7711 | || | | |||| | ||| |||||| ||| |||||||||| |||||||||| .......... ..AAGAGGAT GTACCA-TTT CGAGGGACGT ATCGCGCGCC GCGATGGATA 413 TTATATTTCG AGGGACGTAT CGCGCGCCGC GAAGGTTACT ACTTATCGAG GGTCGTGTCG 7771 ||||||||| ||| |||||| ||||||||| || ||||||| | |||||||| | |||||||| CTATATTTCG AGGAACGTAT CGCGCGCCGT GATGGTTACT A-TTATCGAG GATCGTGTCG 472 TGCGCCGCGA CAGATGCATG GACAGATATG TCCCCCATGG GTCCCGAACT GAGAGACAGC 7831 |||||||||| |||||||||| |||||||||| ||||||| || |||||| ||| |||||||||| TGCGCCGCGA CAGATGCATG GACAGATATG TCCCCCAAGG GTCCCGGACT GAGAGACAGC 532 GGGTGTGTAT CGTTAGGAAA GACATGCATC ACGATATTTG ACATTGCATC TCATGACATT 7891 |||||| ||| | |||| | |||||||||| | ||| ||| ||||||||| ||| |||| GGGTGTATAT CACTAGGTCA GACATGCATC ATTATACTTG ACATTGCATT CCATTGCATT 592 GCACATCTTA TTATTGATGA ACTTGAACAT GTGTTTTGCT GATCTTGTGA TTGTTTCTCT 7951 |||| |||| | ||| ||| |||||| || |||| |||||| | | ||| | ||| ACACA-CTTA TCATTAGTGA ACTTGATATC GTATTTTCTC GATCTTCTTA TTGCCTTTCT 651 G-TGAAGTCT GTGATTGTTG AATATTGAG- TTGATATTGA GAATGTGTAA ACTGATAGAG 8009 | ||| | | ||||||| | ||||||||| ||| ||| | | || | ||| || | || GCAGAACT-T GTGATTGATT AATATTGAGC TTGTTATGGC GGATATATAA TTTG-TTGA. 708 TGTGGTTATT GAGTGGTGTG TTTGGTAAAC TGTTAGGCTG TAGCGTGGAG ATTTAGATAG 8069 .......... .......... .......... .......... .......... .......... 708 GGTGTAAGGA GTACCCGTAT TTTGTTCACT TAACTTGTGT TTAGAGGTTT GCTTGTTGGG 8129 .......... .......... .......... .......... .......... .......... 708 TACCGCGTGG TTTGGTACTC ACCCCTTGCT TCTACAAATT TTTGTAGGTT ACGAGCCTGG 8189 .......... .......... .......... .......... .......... .......... 708 ATCTTTGTGA TACATTCTAT TCTCTTCGTT TTCGAGGCAT CTTGTGGAGG ATTGTGAGGT 8249 .......... .......... .......... .......... .......... .......... 708 AGCTGTTTGT CATCTCAGCG AACTTTCCTA CTCCAGTTTA TGATTTTATT TTTACTTGAA 8309 .......... .......... .......... .......... .......... .......... 708 AGACAACTTC ATTTTAGACT TCTATTTTGT TTCAATTCTA ATGTTTACAT TAGAGGCTTG 8369 .......... .......... .......... .......... .......... .......... 708 TGCACGTGAC AACCTGGTTT TGGGGATTGA ATTTATATGA CTTGGTTTCA TACTAGAAAT 8429 .......... .......... .......... .......... .......... .......... 708 TGTTGTTGGA TTGTCATTTA CTTCCGCACT TTGTTAGATA TTGGGTTTTA GGCTGACTTG 8489 .......... .......... .......... .......... .......... .......... 708 TCTTGGTGGG ATAAGACAAG TGCCATCACA CCTATTTTTG GGTCGTGACA AAATGGTATC 8549 .......... .......... .......... .......... .......... .......... 708 AGAGCCCCAG GTTCATAGGT CTCACGTGTA TACAAGCCGA GTCTACGTAG AGTCTCAAGG 8609 .......... .......... .......... .......... .......... .......... 708 ATCAGTACGG AGACGTCTGT ACTCATCTTT GAGAGGCTAT AAAGATTACT AGGAAACTTC 8669 .......... .......... .......... .......... .......... .......... 708 CTTTTGTTCA TTCTTTCTCA TCGTGCATAG TTACCTTCGT TAGTACTGAG TTGTTGTATA 8729 .......... .......... .......... .......... .......... .......... 708 CTCTTTTGAC AGATGACGAG GACTAGAACT AATGTTACTG GTGGTAGAGG GGAGGCACTT 8789 .......... .......... .......... .......... .......... .......... 708 CCCGAGGCAG TTATTGAGGC CCCAGCTAGA GGTAGGGGTA GATCTCGAGC TAGAGGTCGT 8849 .......... .......... .......... .......... .......... .......... 708 GCTAGTAGTG CGACAGTAAC CAGAGGTCGT GGACGTGGAG CAGCACCAGT GAGAGGTCGT 8909 .......... .......... .......... .......... .......... .......... 708 GCTAGAGAGG TCTCTACCGA ACCACAGATT GATGACAGAG AGGACCAGGT TCCTCCAGAT 8969 .......... .......... .......... .......... .......... .......... 708 CCCGTAGTCA CACCTTTGCT TCAGGATACA CTATTGAGGG TGTTAAGTGT GCTAGAGGGC 9029 .......... .......... .......... .......... .......... .......... 708 TTTTCTCAGG GTGGTGGTGC GACTACCACA CCACATGACT CTCGTACTAG AGAGGGGGCT 9089 .......... .......... .......... .......... .......... .......... 708 CAGACCCAAG AGCAACAACA AGCCCCAGTT TTTCAGGATG CGGTGGGGCA ACTACCAGTA 9149 .......... .......... .......... .......... .......... .......... 708 GATCCCGCGG TTCATAATGA TATTGCACCA GCAATTGGGG GTCAAGTTGC ATCGATGGTT 9209 .......... .......... .......... .......... .......... .......... 708 GTTCTGACAG AGGATGAGCA GCGTAGGTAT GAGAGATTTC GAAAGATGGA CCCACCTCAG 9269 .......... .......... .......... .......... .......... .......... 708 TTTCAGGGTG GGAAGAGCGA GGACGCTCAT GAGTTTCTAA CTACCTGCCG AGAGTTACTA 9329 .......... .......... .......... .......... .......... .......... 708 GAGGTGGTTG GATTAGCTGA GTCACATGGG GTTAGATATG CTACACTCCA GCTTCGTGGA 9389 .......... .......... .......... .......... .......... .......... 708 CCAGCGAGAG ACTGGTGGAG GACTTATTCG GGATGTTTGC CAGTTGGATC TCCTCCAGTG 9449 .......... .......... .......... .......... .......... .......... 708 ACTTGGGAGC AGTTTGCTAG CGCATTCCAA GATCGTTTTA TCCCATGGAG CGTGAGAGAG 9509 .......... .......... .......... .......... .......... .......... 708 GAGAGTCGCT TGAGGTTTGA GAGTCTGAGA CAGGATGGTT TATCGGTTAC AGAGTATGAG 9569 .......... .......... .......... .......... .......... .......... 708 GCGCGTTTTT GCCAGTTGTC TAGGCATGCG TTGGCCATTA TTCCAAATGA GACAGAGAGG 9629 .......... .......... .......... .......... .......... .......... 708 ATCCGCAGAT TCGTGAGGGG ATTGACTTTC TCTATCAGGT CAGCTGTATT TCGGACATCT 9689 .......... .......... .......... .......... .......... .......... 708 AGGGAGGGGA CTTCTTTCCA GTCCATTGTG AGCGCCGCCA AAGAGGCGGA ATTGATGGAG 9749 .......... .......... .......... .......... .......... .......... 708 AGAGAAGAGT TTGGGGACCC TAAAAGAGCT CGTATATCAG GCCAGTTTCA GGGTGCCTCA 9809 .......... .......... .......... .......... .......... .......... 708 TCTGGAGGTA GGGGATCACA GAGAGTGAGT GGTTCTTTTC AGCAGCGGGG ACCTATTCAT 9869 .......... .......... .......... .......... .......... .......... 708 GCATCTATGC CGACATTTGA GGGTGGCCAG ACATCTAGGG GTTCTTATGG CCCAGGTCAG 9929 .......... .......... .......... .......... .......... .......... 708 GGCTCGTACG GTTCACAACA GCGACCTACA GGGCGAGGAA ATTATAGTGG GTTTTCAGGG 9989 .......... .......... .......... .......... .......... .......... 708 TCCACACAAC AGTTCCCAGG TCAGAGATTT TGTTTTACTT GTGGAGATCC CGATCATCTA 10049 .......... .......... .......... .......... .......... .......... 708 ATGCGGCAGT GTACTTCTCA AAGGGGTCGT GGTGGGCCTC GACCAAATTC TTCATTCCAG 10109 .......... .......... .......... .......... .......... .......... 708 ACTAGACCAC CAGCACCACA GGGTAGAGGT CGTGGCAGAG TTCAGTCAGG TAGAGGTGAT 10169 .......... .......... .......... .......... .......... .......... 708 AGAGTTTCCA GTAGTGGTGT TGCAGCTCAG CAGAGTGGGG GTAGAGGTAC CACCCAGGAT 10229 .......... .......... .......... .......... .......... .......... 708 GGAGGTGGAC GAGGAGGCCA CTGCTATGCT TTCCCCGGGA GACCTGAGGC GGAGACCTCA 10289 .......... .......... .......... .......... .......... .......... 708 GATGCGGTTA TTACAGGTAT CATCCCAGTA TGCCATCGAC CTGCGTCTGT GTTGTTTGAT 10349 .......... .......... .......... .......... .......... .......... 708 CCAGGTTCTA CATTCTCTTA TGTGTCTACG TATTTTGCTG CTAAATTTGA TATGATATGT 10409 .......... .......... .......... .......... .......... .......... 708 GATAGCATGA CTGTACCTAT TCGTGTTTCT ACACCCGTGG GTAAGCCCCT AGTGGTGGAT 10469 .......... .......... .......... .......... .......... .......... 708 CGAGTGTATC GATCCTGTCT TGTTTCTTTG GCTGGGTATG ACACTTGGGT AGACTTAATC 10529 || || || .......... .......... .......... .......... .......... ..AC-TATTC 715 ATTCTGGGGA TGGTGGACTT TGATGTTATC TTG 10562 | || ||| | || | || || || | ||| TTGTTGAGGA T-ATGTAATT TGTTGAAGTG TTG 747 hqPGS_C06HBa0016K14.1-4+_SGN-U339313- (7664 8008) ******************************************************************************** EST sequence 5 +strand 681 n (File: SGN-U328762+) 1 CTTGTGGTAT ATGTTTGGGA GTAACGGGAT TGGTGATGGA TTGACTTGTC CACATTGATT 61 AATTTAATGA TGAAAAAAAA AGGATAAAAA AAAAGGCAAT GTGATTAAAT ATTTAAGTGT 121 GATGTGTTCA GAAAGGGATG AAGGATTGTT GAATCATTGT TGATGTGATT TCATGTTTGT 181 GTGGTTTTGA ATTGTGCAAT GTTATGAAAA TGGTCATCTC CTCATTATTT GTGTGAACTT 241 GCCATCTGCA TTATTATGAG GCATTGGTTG TGACAAGTGT TATGTGCATT GAGAAAGAAT 301 GAGTACTTAA GAGGATGTAC AATTTCGAGG GACGTATCGC GCGCCGCGAT GGATACTATA 361 TTTCGAGGGA CGTATCGCAT GCCGCGATGG ATACTATATT TTGAGGGACG TATCGCGCGC 421 CGCGATGGTT ACTATTATCG AGGGTCGTGT CGTGCGCCGC GACAGATGCA TGGACAGATA 481 TGTCCCCCAT GGTTCCCAGA CTGAGAGACA GCGGGTGTGT ATCACTAGGT CAGACATGCA 541 TCATTATACT TGACAATGCA TTACACATAC TTATCATTGG TGAACTTGAT ATTGTGTTTT 601 GCTGATCTTG TGATTGAATT TCTGCGAAAT TTGTGATTGA TGAATACTGA GCTTGTTATT 661 GAGGATATAT AATTTGTTGA A Predicted gene structure (within gDNA segment 3844 to 10032): Exon 1 7666 8003 ( 338 n); cDNA 348 676 ( 329 n); score: 0.840 MATCH C06HBa0016K14.1-4+ SGN-U328762+ 0.840 338 0.496 C PGS_C06HBa0016K14.1-4+_SGN-U328762+ (7666 8003) Alignment (genomic DNA sequence = upper lines): GATGGTGTAC TAT-TTTCGA GGGTCGTATC GCGCGCCGCG ATGGATATTA TATTTCGAGG 7724 ||||| ||| ||| |||||| ||| |||||| || |||||| ||||||| || ||||| |||| GATGG-ATAC TATATTTCGA GGGACGTATC GCATGCCGCG ATGGATACTA TATTTTGAGG 406 GACGTATCGC GCGCCGCGAA GGTTACTACT TATCGAGGGT CGTGTCGTGC GCCGCGACAG 7784 |||||||||| ||||||||| |||||||| | |||||||||| |||||||||| |||||||||| GACGTATCGC GCGCCGCGAT GGTTACTA-T TATCGAGGGT CGTGTCGTGC GCCGCGACAG 465 ATGCATGGAC AGATATGTCC CCCATGGGTC CCGAACTGAG AGACAGCGGG TGTGTATCGT 7844 |||||||||| |||||||||| ||||||| || || |||||| |||||||||| |||||||| ATGCATGGAC AGATATGTCC CCCATGGTTC CCAGACTGAG AGACAGCGGG TGTGTATCAC 525 TAGGAAAGAC ATGCATCACG ATATTTGACA TTGCATCTCA TGACATTGCA CATCTTATTA 7904 |||| |||| |||||||| ||| |||||| |||| | | ||| || | ||||| | TAGGTCAGAC ATGCATCATT ATACTTGACA ATGCA--T-- T-ACA---CA TA-CTTATCA 576 TTGATGAACT TGAACATGTG TTTTGCTGAT CTTGTGATTG TTTCTCTGTG AAGTCTGTGA 7964 ||| |||||| ||| |||| |||||||||| |||||||||| | |||| | || | ||||| TTGGTGAACT TGATATTGTG TTTTGCTGAT CTTGTGATTG AATTTCTGCG AAATTTGTGA 636 TTGTTGAATA TTGAG-TTGA TATTGAGAAT GTGTAAACTG 8003 ||| |||||| |||| ||| ||||||| || | ||| || TTGATGAATA CTGAGCTTGT TATTGAGGAT ATATAATTTG 676 hqPGS_C06HBa0016K14.1-4+_SGN-U328762+ (7666 8003) ******************************************************************************** EST sequence 10 -strand 904 n (File: SGN-U337987-) 1 NACATATCGC GCGCGGCGAT GGATACTATA TTCGAGGGAC GTTCCGCGCG CTGCGATGGT 61 TACTATAATC GAGGGTCGTG TCGTGCGCCG CGACAGATGC ATGGACATAT ATGTCTCCCA 121 TGGGTCCCGG ACTGAGAGAC AGTGGGTGTG TATCACTAGG TCAGACATGC ATCATTATAC 181 TTGACATTGC ATTCCATTGC ATTGCACATA CTTACCATTA GTGAACTTGA TATCGTATTT 241 CGCTGATGTT TTGATTGCTT TTCTGCGGAA CTTGTGATTG ATCAATATTG AGCTTGTTAT 301 TGCGGATATG TAATTTGTTG AAGTATTGTT GTTGAGGATA TGTAATTTGT TGAAGTATTG 361 TTGTTGAGGA TATGTAATTT GTTGAAGTGT TGTTGTTGAG GATATGTAAT TTGCCTAAGT 421 GTTGTTGTTG AGCTATGTGC TATGTAAATT GTGAGCTGTT AGGTTGGGTT GATTTTAATG 481 CAGGTTGTAT TTGTGGAGGT TCGGTTGGGG GTGGTAGGAG TACCCGTATT TCATCCCCTT 541 AGCTTATGTT TAGAGGTTTA CTTGCTGAGT ACCGTGTGGT TTGGTACTCA CCCCTTGCTT 601 CTACAAATTT TTGTAGGTTA TGAGCCTGGA TTTTTGTTGT ACTTGTCAGT CTCTTCTTTT 661 CCGAGGCTTC ATGGAGATTT GTGAGGTAGC TGTTTCCATC TCAGCAGACT TTCTTCTCCA 721 TAATTATGAT CTTGTTCTAT TCTAGAAACA AGTTCATTTG AGACTTGAGT TTTTTTCTTT 781 TGAATCTTGT AATACTTTAG AGGCTTGTAC ACGTGACTAC CCAGGTCTTG GGGGTTTAAT 841 TAAGTTACTT ATATTTTATT TCTGCAAAAA AAAAAAAAAA AAACTCGAGG GGGGGCCCGG 901 TCCC Predicted gene structure (within gDNA segment 6476 to 12220): Exon 1 7691 8030 ( 340 n); cDNA 5 343 ( 339 n); score: 0.809 Intron 1 8031 11065 (3035 n); Pd: 0.000 (s: 0.70), Pa: 0.623 (s: 0) Exon 2 11066 11078 ( 13 n); cDNA 344 355 ( 12 n); score: 0.615 PPA cDNA 866 884 MATCH C06HBa0016K14.1-4+ SGN-U337987- 0.809 353 0.390 C PGS_C06HBa0016K14.1-4+_SGN-U337987- (7691 8030,11066 11078) Alignment (genomic DNA sequence = upper lines): TATCGCGCGC CGCGATGGAT ATTATATTTC GAGGGACGTA TCGCGCGCCG CGAAGGTTAC 7750 |||||||||| ||||||||| | |||| ||| ||||||||| ||||||| | ||| |||||| TATCGCGCGC GGCGATGGAT ACTATA-TTC GAGGGACGTT CCGCGCGCTG CGATGGTTAC 63 TACTTATCGA GGGTCGTGTC GTGCGCCGCG ACAGATGCAT GGACAGATAT GTCCCCCATG 7810 || | ||||| |||||||||| |||||||||| |||||||||| ||||| |||| ||| |||||| TA-TAATCGA GGGTCGTGTC GTGCGCCGCG ACAGATGCAT GGACATATAT GTCTCCCATG 122 GGTCCCGAAC TGAGAGACAG CGGGTGTGTA TCGTTAGGAA AGACATGCAT CACGATATTT 7870 ||||||| || |||||||||| ||||||||| || |||| |||||||||| || ||| || GGTCCCGGAC TGAGAGACAG TGGGTGTGTA TCACTAGGTC AGACATGCAT CATTATACTT 182 GACATTGCAT CTCATGACAT TGCACAT-CT TATTATTGAT GAACTTGAAC ATGTGTTTTG 7929 |||||||||| ||| ||| ||||||| || || ||| | |||||||| || ||| | GACATTGCAT TCCATTGCAT TGCACATACT TACCATTAGT GAACTTGATA TCGTATTTCG 242 CTGATCTTGT GATTGTTTCT CTGTGAAGTC TGTGATTGTT GAATATTGAG -TTGATATTG 7988 ||||| || | ||||| || | ||| | | |||||||| | ||||||||| ||| ||||| CTGATGTTTT GATTGCTTTT CTGCGGAACT TGTGATTGAT CAATATTGAG CTTGTTATTG 302 AGAATGTGTA AACTGATAGA GTGTGGTTAT TGAGTGGTGT GTTTGGTAAA CTGTTAGGCT 8048 | || |||| | || | | || | ||| | |||| | | | || CGGATATGTA ATTTGTTGAA GTATTGTTGT TGAG-GATAT GT........ .......... 343 GTAGCGTGGA GATTTAGATA GGGTGTAAGG AGTACCCGTA TTTTGTTCAC TTAACTTGTG 8108 .......... .......... .......... .......... .......... .......... 343 TTTAGAGGTT TGCTTGTTGG GTACCGCGTG GTTTGGTACT CACCCCTTGC TTCTACAAAT 8168 .......... .......... .......... .......... .......... .......... 343 TTTTGTAGGT TACGAGCCTG GATCTTTGTG ATACATTCTA TTCTCTTCGT TTTCGAGGCA 8228 .......... .......... .......... .......... .......... .......... 343 TCTTGTGGAG GATTGTGAGG TAGCTGTTTG TCATCTCAGC GAACTTTCCT ACTCCAGTTT 8288 .......... .......... .......... .......... .......... .......... 343 ATGATTTTAT TTTTACTTGA AAGACAACTT CATTTTAGAC TTCTATTTTG TTTCAATTCT 8348 .......... .......... .......... .......... .......... .......... 343 AATGTTTACA TTAGAGGCTT GTGCACGTGA CAACCTGGTT TTGGGGATTG AATTTATATG 8408 .......... .......... .......... .......... .......... .......... 343 ACTTGGTTTC ATACTAGAAA TTGTTGTTGG ATTGTCATTT ACTTCCGCAC TTTGTTAGAT 8468 .......... .......... .......... .......... .......... .......... 343 ATTGGGTTTT AGGCTGACTT GTCTTGGTGG GATAAGACAA GTGCCATCAC ACCTATTTTT 8528 .......... .......... .......... .......... .......... .......... 343 GGGTCGTGAC AAAATGGTAT CAGAGCCCCA GGTTCATAGG TCTCACGTGT ATACAAGCCG 8588 .......... .......... .......... .......... .......... .......... 343 AGTCTACGTA GAGTCTCAAG GATCAGTACG GAGACGTCTG TACTCATCTT TGAGAGGCTA 8648 .......... .......... .......... .......... .......... .......... 343 TAAAGATTAC TAGGAAACTT CCTTTTGTTC ATTCTTTCTC ATCGTGCATA GTTACCTTCG 8708 .......... .......... .......... .......... .......... .......... 343 TTAGTACTGA GTTGTTGTAT ACTCTTTTGA CAGATGACGA GGACTAGAAC TAATGTTACT 8768 .......... .......... .......... .......... .......... .......... 343 GGTGGTAGAG GGGAGGCACT TCCCGAGGCA GTTATTGAGG CCCCAGCTAG AGGTAGGGGT 8828 .......... .......... .......... .......... .......... .......... 343 AGATCTCGAG CTAGAGGTCG TGCTAGTAGT GCGACAGTAA CCAGAGGTCG TGGACGTGGA 8888 .......... .......... .......... .......... .......... .......... 343 GCAGCACCAG TGAGAGGTCG TGCTAGAGAG GTCTCTACCG AACCACAGAT TGATGACAGA 8948 .......... .......... .......... .......... .......... .......... 343 GAGGACCAGG TTCCTCCAGA TCCCGTAGTC ACACCTTTGC TTCAGGATAC ACTATTGAGG 9008 .......... .......... .......... .......... .......... .......... 343 GTGTTAAGTG TGCTAGAGGG CTTTTCTCAG GGTGGTGGTG CGACTACCAC ACCACATGAC 9068 .......... .......... .......... .......... .......... .......... 343 TCTCGTACTA GAGAGGGGGC TCAGACCCAA GAGCAACAAC AAGCCCCAGT TTTTCAGGAT 9128 .......... .......... .......... .......... .......... .......... 343 GCGGTGGGGC AACTACCAGT AGATCCCGCG GTTCATAATG ATATTGCACC AGCAATTGGG 9188 .......... .......... .......... .......... .......... .......... 343 GGTCAAGTTG CATCGATGGT TGTTCTGACA GAGGATGAGC AGCGTAGGTA TGAGAGATTT 9248 .......... .......... .......... .......... .......... .......... 343 CGAAAGATGG ACCCACCTCA GTTTCAGGGT GGGAAGAGCG AGGACGCTCA TGAGTTTCTA 9308 .......... .......... .......... .......... .......... .......... 343 ACTACCTGCC GAGAGTTACT AGAGGTGGTT GGATTAGCTG AGTCACATGG GGTTAGATAT 9368 .......... .......... .......... .......... .......... .......... 343 GCTACACTCC AGCTTCGTGG ACCAGCGAGA GACTGGTGGA GGACTTATTC GGGATGTTTG 9428 .......... .......... .......... .......... .......... .......... 343 CCAGTTGGAT CTCCTCCAGT GACTTGGGAG CAGTTTGCTA GCGCATTCCA AGATCGTTTT 9488 .......... .......... .......... .......... .......... .......... 343 ATCCCATGGA GCGTGAGAGA GGAGAGTCGC TTGAGGTTTG AGAGTCTGAG ACAGGATGGT 9548 .......... .......... .......... .......... .......... .......... 343 TTATCGGTTA CAGAGTATGA GGCGCGTTTT TGCCAGTTGT CTAGGCATGC GTTGGCCATT 9608 .......... .......... .......... .......... .......... .......... 343 ATTCCAAATG AGACAGAGAG GATCCGCAGA TTCGTGAGGG GATTGACTTT CTCTATCAGG 9668 .......... .......... .......... .......... .......... .......... 343 TCAGCTGTAT TTCGGACATC TAGGGAGGGG ACTTCTTTCC AGTCCATTGT GAGCGCCGCC 9728 .......... .......... .......... .......... .......... .......... 343 AAAGAGGCGG AATTGATGGA GAGAGAAGAG TTTGGGGACC CTAAAAGAGC TCGTATATCA 9788 .......... .......... .......... .......... .......... .......... 343 GGCCAGTTTC AGGGTGCCTC ATCTGGAGGT AGGGGATCAC AGAGAGTGAG TGGTTCTTTT 9848 .......... .......... .......... .......... .......... .......... 343 CAGCAGCGGG GACCTATTCA TGCATCTATG CCGACATTTG AGGGTGGCCA GACATCTAGG 9908 .......... .......... .......... .......... .......... .......... 343 GGTTCTTATG GCCCAGGTCA GGGCTCGTAC GGTTCACAAC AGCGACCTAC AGGGCGAGGA 9968 .......... .......... .......... .......... .......... .......... 343 AATTATAGTG GGTTTTCAGG GTCCACACAA CAGTTCCCAG GTCAGAGATT TTGTTTTACT 10028 .......... .......... .......... .......... .......... .......... 343 TGTGGAGATC CCGATCATCT AATGCGGCAG TGTACTTCTC AAAGGGGTCG TGGTGGGCCT 10088 .......... .......... .......... .......... .......... .......... 343 CGACCAAATT CTTCATTCCA GACTAGACCA CCAGCACCAC AGGGTAGAGG TCGTGGCAGA 10148 .......... .......... .......... .......... .......... .......... 343 GTTCAGTCAG GTAGAGGTGA TAGAGTTTCC AGTAGTGGTG TTGCAGCTCA GCAGAGTGGG 10208 .......... .......... .......... .......... .......... .......... 343 GGTAGAGGTA CCACCCAGGA TGGAGGTGGA CGAGGAGGCC ACTGCTATGC TTTCCCCGGG 10268 .......... .......... .......... .......... .......... .......... 343 AGACCTGAGG CGGAGACCTC AGATGCGGTT ATTACAGGTA TCATCCCAGT ATGCCATCGA 10328 .......... .......... .......... .......... .......... .......... 343 CCTGCGTCTG TGTTGTTTGA TCCAGGTTCT ACATTCTCTT ATGTGTCTAC GTATTTTGCT 10388 .......... .......... .......... .......... .......... .......... 343 GCTAAATTTG ATATGATATG TGATAGCATG ACTGTACCTA TTCGTGTTTC TACACCCGTG 10448 .......... .......... .......... .......... .......... .......... 343 GGTAAGCCCC TAGTGGTGGA TCGAGTGTAT CGATCCTGTC TTGTTTCTTT GGCTGGGTAT 10508 .......... .......... .......... .......... .......... .......... 343 GACACTTGGG TAGACTTAAT CATTCTGGGG ATGGTGGACT TTGATGTTAT CTTGGGTATG 10568 .......... .......... .......... .......... .......... .......... 343 GATTGGCTTT CTCCTTATCA TGCTGTCCTT GATTGTAATG CTAAGACTGT GACTTTAGCA 10628 .......... .......... .......... .......... .......... .......... 343 ATGCCTGGTG TTCCGAGGGT TGAGTGGAAG AGTGTTAGTG GTTCTTATCC TAGAAAGGTT 10688 .......... .......... .......... .......... .......... .......... 343 ATCTCTTTTA TCCGTGCACA GAGATTGGTG GAGAGGGGGT GTTTGTCTTA CTTAGCTTTT 10748 .......... .......... .......... .......... .......... .......... 343 ATTCGGGATA CTAGTGTTGA ACCACCTTCC ATGGACTCTG TTCCCGTGGT TCAGGAGTTT 10808 .......... .......... .......... .......... .......... .......... 343 CTCGATGTAT TTCCTTCTGA TCTTCCAGGT GTTCCTCCCG ATAGGGACAT TGATTTTGCT 10868 .......... .......... .......... .......... .......... .......... 343 ATTGATTTGG AGCCAGGCAC TAAGCCTATT TCTATACCTC CATATCGTAT GGCCCCAGCA 10928 .......... .......... .......... .......... .......... .......... 343 GAGTTGAAAG AATTAAAGGA TCAGTTGCAA GATTTATTGA GTAAGGGTTT TATTCGCCCT 10988 .......... .......... .......... .......... .......... .......... 343 AGTGTATCAC CTTGGGGTGC CCCTGTATTG TTTGTGAAGA AAAAGGATGG GACTATGAGA 11048 .......... .......... .......... .......... .......... .......... 343 ATGTGTATTG ATTACAGACA GTTGAACAAG 11078 | | ||| ||| .......... .......A-A TTTGTTGAAG 355 hqPGS_C06HBa0016K14.1-4+_SGN-U337987- (7691 8030) ******************************************************************************** EST sequence 9 -strand 805 n (File: SGN-U344270-) 1 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 61 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 121 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 181 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTGCC ATTCGCCATT 241 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 301 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC 361 ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGG 421 TACCGGGCCC CCCCTCGAGG CAGTTGTTGA GGCCCCAGTT ATGGGTAGGG GTAGGTCTCG 481 AGCTAGATGT CGTTCTAGTA GTACGATAGT AGCTAGAGGT CGTGGACGTG GAGCAGCACC 541 AGTGAGAGGT CGTGCTAGAG AGGTCTCTAC AAAACCTCAA ATTGATGGCA GAGGTGACCA 601 GGTTCCTCCA GATCCTGTAG TCACACCTTT GCTTCAGGAT ACACTTTTTA GGGTGTTAAG 661 TGTTCTAGAG GGCTTTTATC AGGGTGGTGG TGCGACTACC ACACCACATG ACTCTCGTAC 721 TAGAGAGGGT GCTCCGACCC AACCTGCAGC CCGGGGGATC CACTAGTTCT AGAGCGGCCG 781 CAACCGCGGT GGAGCTCCAG CTTAT Predicted gene structure (within gDNA segment 3121 to 10445): Exon 1 8792 9098 ( 307 n); cDNA 436 742 ( 307 n); score: 0.919 MATCH C06HBa0016K14.1-4+ SGN-U344270- 0.919 307 0.381 C PGS_C06HBa0016K14.1-4+_SGN-U344270- (8792 9098) Alignment (genomic DNA sequence = upper lines): CGAGGCAGTT ATTGAGGCCC CAGCTAGAGG TAGGGGTAGA TCTCGAGCTA GAGGTCGTGC 8851 |||||||||| ||||||||| ||| || || ||||||||| |||||||||| || ||||| | CGAGGCAGTT GTTGAGGCCC CAGTTATGGG TAGGGGTAGG TCTCGAGCTA GATGTCGTTC 495 TAGTAGTGCG ACAGTAACCA GAGGTCGTGG ACGTGGAGCA GCACCAGTGA GAGGTCGTGC 8911 ||||||| || | |||| | | |||||||||| |||||||||| |||||||||| |||||||||| TAGTAGTACG ATAGTAGCTA GAGGTCGTGG ACGTGGAGCA GCACCAGTGA GAGGTCGTGC 555 TAGAGAGGTC TCTACCGAAC CACAGATTGA TGACAGAGAG GACCAGGTTC CTCCAGATCC 8971 |||||||||| ||||| ||| | || ||||| || ||||| |||||||||| |||||||||| TAGAGAGGTC TCTACAAAAC CTCAAATTGA TGGCAGAGGT GACCAGGTTC CTCCAGATCC 615 CGTAGTCACA CCTTTGCTTC AGGATACACT ATTGAGGGTG TTAAGTGTGC TAGAGGGCTT 9031 ||||||||| |||||||||| |||||||||| || |||||| |||||||| | |||||||||| TGTAGTCACA CCTTTGCTTC AGGATACACT TTTTAGGGTG TTAAGTGTTC TAGAGGGCTT 675 TTCTCAGGGT GGTGGTGCGA CTACCACACC ACATGACTCT CGTACTAGAG AGGGGGCTCA 9091 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| |||| TTATCAGGGT GGTGGTGCGA CTACCACACC ACATGACTCT CGTACTAGAG AGGGTGCTCC 735 GACCCAA 9098 ||||||| GACCCAA 742 hqPGS_C06HBa0016K14.1-4+_SGN-U344270- (8792 9098) ******************************************************************************** EST sequence 6 +strand 885 n (File: SGN-U339521+) 1 TGGTGATGAA TGATGACAAT CGTTGGAGCT CCCGCGCCTG TAGGTCGACA CTAGTGGATC 61 CAAAGAATTC GGCACGAGTT GAGAGTCTGA GACAGGATGG TTTATCGGTT ACAGAGTGTA 121 AGGCGCGTTT TTGCCAGTTG TCTAGGCATG CGTTGGCCAT TATTCCAAAT GAAACAGAGA 181 GGATCCGTAG ATTTGTGAGG GGATTGACTT TCTCTATCAG GTCAGCTGTG TTTCGGACAT 241 CTAGGGGGGA CTTCTTTCCA GTCTATTGTG AGCGCCGCCA AAGAGGCAGA ATTGATGGAG 301 AGAGAGGAGT TTGGGGACCC TAAAAGAGCT CGTATATCAG GTCAATTTCA GGGTGCCTCA 361 TCTGGAGGTA GGGGATCACA GAGAGTGAGT GGTTCTTTTC AGCAGCGGGG ACCTATTCAT 421 GCATCTATGC CGACATTTGA GGGTGGCCAG ACATCTAAGG GTTCTTATGG CCCGGGTCAT 481 GGCTCGTACG GTTCACAGCA GCGACCTTCA GGGCGAGGCA ACTTTTGTGG GTTTTTAGGG 541 TTCACACAGT TGTTTTCCAT GTCAAAAATT TTGCTCTACT TGGTGGAGAT CCCGATTATA 601 TTATTGCTGG CTATATGTCT TTCTCAAAGG AGTTCTAGAT GGAGCCTTCT ATCTATAATA 661 CTTTATTTTT CATATCTTTA TACACCGTAG TTCTCCGCAT GGTAATAAGT CTATTTGTCC 721 ATAATTTCTC GTTATGTTAC AATGTGTATT GAGTCATCTT ATCTTGTTGT ATGATACATA 781 TTTATACGTG ATGTTTGAGT CTATGTTATC TATCTCAATT TAAAGTGGAT GGATAGATGA 841 TTATGTATCT CTATATTATA TACTACTATG GTGTTTAGTA ATGGC Predicted gene structure (within gDNA segment 8146 to 14796): Exon 1 9526 10081 ( 556 n); cDNA 79 638 ( 560 n); score: 0.896 Intron 1 10082 12849 (2768 n); Pd: 0.000 (s: 0.57), Pa: 0.344 (s: 0) Exon 2 12850 12855 ( 6 n); cDNA 639 644 ( 6 n); score: 0.833 MATCH C06HBa0016K14.1-4+ SGN-U339521+ 0.896 562 0.635 C PGS_C06HBa0016K14.1-4+_SGN-U339521+ (9526 10081,12850 12855) Alignment (genomic DNA sequence = upper lines): TTGAGAGTCT GAGACAGGAT GGTTTATCGG TTACAGAGTA TGAGGCGCGT TTTTGCCAGT 9585 |||||||||| |||||||||| |||||||||| ||||||||| | |||||||| |||||||||| TTGAGAGTCT GAGACAGGAT GGTTTATCGG TTACAGAGTG TAAGGCGCGT TTTTGCCAGT 138 TGTCTAGGCA TGCGTTGGCC ATTATTCCAA ATGAGACAGA GAGGATCCGC AGATTCGTGA 9645 |||||||||| |||||||||| |||||||||| |||| ||||| ||||||||| ||||| |||| TGTCTAGGCA TGCGTTGGCC ATTATTCCAA ATGAAACAGA GAGGATCCGT AGATTTGTGA 198 GGGGATTGAC TTTCTCTATC AGGTCAGCTG TATTTCGGAC ATCTAGGGAG GGGACTTCTT 9705 |||||||||| |||||||||| |||||||||| | |||||||| ||||| || | |||||||||| GGGGATTGAC TTTCTCTATC AGGTCAGCTG TGTTTCGGAC ATCTA-GG-G GGGACTTCTT 256 TCCAGTCCAT TGTGAGCGCC GCCAAAGAGG CGGAATTGAT GGAGAGAGAA GAGTTTGGGG 9765 ||||||| || |||||||||| |||||||||| | |||||||| ||||||||| |||||||||| TCCAGTCTAT TGTGAGCGCC GCCAAAGAGG CAGAATTGAT GGAGAGAGAG GAGTTTGGGG 316 ACCCTAAAAG AGCTCGTATA TCAGGCCAGT TTCAGGGTGC CTCATCTGGA GGTAGGGGAT 9825 |||||||||| |||||||||| ||||| || | |||||||||| |||||||||| |||||||||| ACCCTAAAAG AGCTCGTATA TCAGGTCAAT TTCAGGGTGC CTCATCTGGA GGTAGGGGAT 376 CACAGAGAGT GAGTGGTTCT TTTCAGCAGC GGGGACCTAT TCATGCATCT ATGCCGACAT 9885 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACAGAGAGT GAGTGGTTCT TTTCAGCAGC GGGGACCTAT TCATGCATCT ATGCCGACAT 436 TTGAGGGTGG CCAGACATCT AGGGGTTCTT ATGGCCCAGG TCAGGGCTCG TACGGTTCAC 9945 |||||||||| |||||||||| | |||||||| ||||||| || ||| |||||| |||||||||| TTGAGGGTGG CCAGACATCT AAGGGTTCTT ATGGCCCGGG TCATGGCTCG TACGGTTCAC 496 AACAGCGACC TACAGGGCGA GGAAATTATA GTGGGTTTTC AGGGTCCACA CAACAG-TTC 10004 | |||||||| | |||||||| || || | | ||||||||| ||||| |||| || | || AGCAGCGACC TTCAGGGCGA GGCAACTTTT GTGGGTTTTT AGGGTTCACA CAGTTGTTTT 556 CCAGGTCAGA GATTTTGTTT TACTT-GTGG AGATCCCGAT CAT-CTAATG C-GGC-A-GT 10059 ||| |||| | |||||| | ||||| |||| |||||||||| || || || | ||| | | CCATGTCAAA AATTTTGCTC TACTTGGTGG AGATCCCGAT TATATTATTG CTGGCTATAT 616 GTACTTCTCA AAGGGGTCGT GGTGGGCCTC GACCAAATTC TTCATTCCAG ACTAGACCAC 10119 || |||||| |||| || | | GTCTTTCTCA AAGGAGTTCT AG........ .......... .......... .......... 638 CAGCACCACA GGGTAGAGGT CGTGGCAGAG TTCAGTCAGG TAGAGGTGAT AGAGTTTCCA 10179 .......... .......... .......... .......... .......... .......... 638 GTAGTGGTGT TGCAGCTCAG CAGAGTGGGG GTAGAGGTAC CACCCAGGAT GGAGGTGGAC 10239 .......... .......... .......... .......... .......... .......... 638 GAGGAGGCCA CTGCTATGCT TTCCCCGGGA GACCTGAGGC GGAGACCTCA GATGCGGTTA 10299 .......... .......... .......... .......... .......... .......... 638 TTACAGGTAT CATCCCAGTA TGCCATCGAC CTGCGTCTGT GTTGTTTGAT CCAGGTTCTA 10359 .......... .......... .......... .......... .......... .......... 638 CATTCTCTTA TGTGTCTACG TATTTTGCTG CTAAATTTGA TATGATATGT GATAGCATGA 10419 .......... .......... .......... .......... .......... .......... 638 CTGTACCTAT TCGTGTTTCT ACACCCGTGG GTAAGCCCCT AGTGGTGGAT CGAGTGTATC 10479 .......... .......... .......... .......... .......... .......... 638 GATCCTGTCT TGTTTCTTTG GCTGGGTATG ACACTTGGGT AGACTTAATC ATTCTGGGGA 10539 .......... .......... .......... .......... .......... .......... 638 TGGTGGACTT TGATGTTATC TTGGGTATGG ATTGGCTTTC TCCTTATCAT GCTGTCCTTG 10599 .......... .......... .......... .......... .......... .......... 638 ATTGTAATGC TAAGACTGTG ACTTTAGCAA TGCCTGGTGT TCCGAGGGTT GAGTGGAAGA 10659 .......... .......... .......... .......... .......... .......... 638 GTGTTAGTGG TTCTTATCCT AGAAAGGTTA TCTCTTTTAT CCGTGCACAG AGATTGGTGG 10719 .......... .......... .......... .......... .......... .......... 638 AGAGGGGGTG TTTGTCTTAC TTAGCTTTTA TTCGGGATAC TAGTGTTGAA CCACCTTCCA 10779 .......... .......... .......... .......... .......... .......... 638 TGGACTCTGT TCCCGTGGTT CAGGAGTTTC TCGATGTATT TCCTTCTGAT CTTCCAGGTG 10839 .......... .......... .......... .......... .......... .......... 638 TTCCTCCCGA TAGGGACATT GATTTTGCTA TTGATTTGGA GCCAGGCACT AAGCCTATTT 10899 .......... .......... .......... .......... .......... .......... 638 CTATACCTCC ATATCGTATG GCCCCAGCAG AGTTGAAAGA ATTAAAGGAT CAGTTGCAAG 10959 .......... .......... .......... .......... .......... .......... 638 ATTTATTGAG TAAGGGTTTT ATTCGCCCTA GTGTATCACC TTGGGGTGCC CCTGTATTGT 11019 .......... .......... .......... .......... .......... .......... 638 TTGTGAAGAA AAAGGATGGG ACTATGAGAA TGTGTATTGA TTACAGACAG TTGAACAAGG 11079 .......... .......... .......... .......... .......... .......... 638 TAACAATTAA GAATAAGTAT CCTCTTCCGC GTATTGATGA CTTATTTGAT CAGTTACAGG 11139 .......... .......... .......... .......... .......... .......... 638 GAGCATCATT GTTCTCTAAG ATTGATTTGA GGTCTGGGTA TCATCAGTTG AAAATTAGGG 11199 .......... .......... .......... .......... .......... .......... 638 CATCAGATAT CCCTAAGACA GCTTTTCGGA CCCGGTATGG GCATTATGAG TTTCTAGTGA 11259 .......... .......... .......... .......... .......... .......... 638 TGTCCTTCGG ATTGACTAAT GCCCCTGCAG CATTCATGGA GTTGATGAAT GGGGTGTTTC 11319 .......... .......... .......... .......... .......... .......... 638 GACCATACCT TGATTCTTTT GTGATTGTTT TCATCGATGA CATCTTGGTT TACTCTAAGA 11379 .......... .......... .......... .......... .......... .......... 638 CTGAGGAGGA CCATGTCCGA CACCTGAGGA TTGTACTCCA GAGGTTGAGA GAAGAGAAGT 11439 .......... .......... .......... .......... .......... .......... 638 TGTATGCCAA GTTCTCAAAG TGTGAGTTCT GGCTTACTTC TGTGACATTC TTGGGGCACG 11499 .......... .......... .......... .......... .......... .......... 638 TGGTGTCCAA GGAGGGTATT AGAGTAGATC CAGCCAAAAT TAAGGCAGTT AGAGGCTGGA 11559 .......... .......... .......... .......... .......... .......... 638 CGCGACCTAC CTCACCTACT GAGATTAGGA GTTTTGTGGG ATTGGCAGGC TATTATCGAC 11619 .......... .......... .......... .......... .......... .......... 638 GATTTGTTCA GAGTTTCTCT ACTATTGCAG CTCCATTGAC TAGATTGACT CGACAGGATG 11679 .......... .......... .......... .......... .......... .......... 638 TGAGTTTTCA GTGGTCTGAT GAGTGTGAGG AGAGCTTTCA AAAACTCAAG ACTTTGTTGA 11739 .......... .......... .......... .......... .......... .......... 638 CTTCTGCTCC TGTGTTGACT CTACCTGAGG AAGGTGTAGA CTTTACTGTG TATTGTGATG 11799 .......... .......... .......... .......... .......... .......... 638 CTTCAGGAGT TGGTTTGGGT GGTGTGTTGA TGCAGAAGGG GAAAGTGATT GCTTATGCTT 11859 .......... .......... .......... .......... .......... .......... 638 CTAGGCAATT GAAATCCCAT GAGAAAAACT ACCCTACTCA TGATTTGGAG TTGGCGGCTG 11919 .......... .......... .......... .......... .......... .......... 638 TGGTATTTGT ACTTAAGTTA TGGCGTCATT ATTTGTATGG GGTGCATTGT GAGATCTTCA 11979 .......... .......... .......... .......... .......... .......... 638 CTGATCATCG GAGTCTTCAA TATATCTTTA GCTAGAGGGA TTTGAACTTG AGGCAACGGA 12039 .......... .......... .......... .......... .......... .......... 638 GATGGCTTGA GTTGTTGAAG GACTATGATG TGACCATTCT GTATCATCCA GGAAAGGCCA 12099 .......... .......... .......... .......... .......... .......... 638 ATGTTATGGC CGATGCTCTG AGTAGGAAGA CTCCTAGCAT GGGGAGTCTT GCAGCACTTA 12159 .......... .......... .......... .......... .......... .......... 638 GTATTATGGA GAGACCATTG GCTAGAGATG TGCAGATATT AGCTAACAGT CTTGTCCGCT 12219 .......... .......... .......... .......... .......... .......... 638 TGCAGATTTC AGAAGAGAGT GAGGGGATGA TTGCTTTTAT TGAGGCTCGG TCTTCTTTAG 12279 .......... .......... .......... .......... .......... .......... 638 TCGAGCAAAT TCGTGCACAC CAATTTGATG ACGAAAAATT ATGTCTCATT CGAGACAGAG 12339 .......... .......... .......... .......... .......... .......... 638 TATTGAGAGG GGAAGCTAAG GAGGCTGTCC TTGATTCTGA TGGTGTCTTG CGGATCGGAG 12399 .......... .......... .......... .......... .......... .......... 638 GCAGAATTTG TGTGCCCAGG ACAGGCGATT TGATTAGATT GATTCTTGAG GAGGCCCATT 12459 .......... .......... .......... .......... .......... .......... 638 GTACTCGGTA TTCCATCCAT CCGGGAGCGG CAAAGATGTA TCATGATCTG AGTCAGCATT 12519 .......... .......... .......... .......... .......... .......... 638 ACTGGTGGTG TGGGATGAAG AGAGATATTT CAGACTTTGT TTCTAGGTGT TTGACTTGCC 12579 .......... .......... .......... .......... .......... .......... 638 AGCAGGTCAA ATGTGAGCAC CAGCGGCCAG GGGGTGTATC TCAGAGGATG CCTATTCCTA 12639 .......... .......... .......... .......... .......... .......... 638 CTTGGAAGTG GGAGCGGATT ACTATGGACT TTGTTGTGGG TTTGCCTACC ACAGTGGGTG 12699 .......... .......... .......... .......... .......... .......... 638 GTTATGACTC TATTTGGGTT GTTGTTGATA GACTGACCAA GTCTGCCCAC TTCATCCCGG 12759 .......... .......... .......... .......... .......... .......... 638 TTCGGGTAAA GTATACAGCA GAAAAGTTAG CCGAACTATA TATCAGTCAG ATTGTACGAC 12819 .......... .......... .......... .......... .......... .......... 638 TACATGGAGT TCCTCTTTCT ATCATATCAG ATCGAG 12855 || ||| .......... .......... .......... ATGGAG 644 hqPGS_C06HBa0016K14.1-4+_SGN-U339521+ (9526 10081) ******************************************************************************** EST sequence 14 -strand 852 n (File: SGN-U339520-) 1 AGCTGATTTA ACAAAAATTT AACGCGAATT TAACAAAATA TAAACGCTTA CAATTTGCAT 61 TCGCCATTCA GGCTGCGCAA CTGTGGGAAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA 121 CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT 181 TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC 241 GAATTGGGTA CCGGGCCCCC CCTCGAGTTT TTTTTCCAGT CCATTATGAG CGCCTCCAAA 301 GAGTTGGAAT TGATGAAGAG AGGGAGGAGT TTGGGGACCC TATAAGGGCC CGTATATCAA 361 GTCAGTTTCA TGGTGATTCA TCTGGAGGTA GGGGATCACA GAGAGTGAGT GGTTCTTTTC 421 AGCAGCGGGG ACCTATTCAT GCATCTAAGA CAATATTTGA GGGTGGCCAA ACATCTAGGG 481 GTTCTTATGT CCTGGTTCAG GGCTCGTACG GTTCACAGCA GCGACCTTCA GGGCGAGGCA 541 ATTATTGTGG GTTTTCAGGG TCCACACAGC AGTTCCCAGG TTAGAGATTT TGTTTTACTT 601 GTGGAGATCC CGATCATCTA ATGCGGCAGT GTACTTCTTA AAGGGGTCGT GGTGGGCCTC 661 GACCTAATTC TTCATTCCAT ACTAGACCAC TAGCTCTACA GGGTAGAGGT CGTTGCAGAG 721 TACAGTCAGG TAGGGTGATA GAGTTTCTAG TAGTGGTGTT GGAGCTCAGC AAAGTTGGGG 781 TAGAGGTACC ACCCAACTGC AGCCCGGGGG ATCCACTAGT TAGTATAGCG GCCGCCACCG 841 CGGGGAGCTC CA Predicted gene structure (within gDNA segment 5427 to 12573): Exon 1 9701 10225 ( 525 n); cDNA 270 795 ( 526 n); score: 0.909 MATCH C06HBa0016K14.1-4+ SGN-U339520- 0.909 525 0.616 C PGS_C06HBa0016K14.1-4+_SGN-U339520- (9701 10225) Alignment (genomic DNA sequence = upper lines): TTCTTTCCAG TCCATTGTGA GCGCCGCCAA AGAGGCGGAA TTGATG--GA GAGAGAAGAG 9758 || ||||||| |||||| ||| ||||| |||| |||| |||| |||||| || ||| || ||| TTTTTTCCAG TCCATTATGA GCGCCTCCAA AGAGTTGGAA TTGATGAAGA GAGGGAGGAG 329 TTTGGGGACC CTAAAAGAGC TCGTATATCA GGCCAGTTTC AGGGTGCCTC ATCTGGAGGT 9818 |||||||||| ||| ||| || ||||||||| | ||||||| | |||| || |||||||||| TTTGGGGACC CTATAAGGGC CCGTATATCA AGTCAGTTTC ATGGTGATTC ATCTGGAGGT 389 AGGGGATCAC AGAGAGTGAG TGGTTCTTTT CAGCAGCGGG GACCTATTCA TGCATCTATG 9878 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| | AGGGGATCAC AGAGAGTGAG TGGTTCTTTT CAGCAGCGGG GACCTATTCA TGCATCTAAG 449 CCGACATTTG AGGGTGGCCA GACATCTAGG GGTTCTTATG GCCCAGGTCA GGGCTCGTAC 9938 | | ||||| |||||||||| ||||||||| |||||||||| || | ||| |||||||||| ACAATATTTG AGGGTGGCCA AACATCTAGG GGTTCTTATG TCCTGGTTCA GGGCTCGTAC 509 GGTTCACAAC AGCGACCTAC AGGGCGAGGA AATTATAGTG GGTTTTCAGG GTCCACACAA 9998 |||||||| | |||||||| | ||||||||| |||||| ||| |||||||||| ||||||||| GGTTCACAGC AGCGACCTTC AGGGCGAGGC AATTATTGTG GGTTTTCAGG GTCCACACAG 569 CAGTTCCCAG GTCAGAGATT TTGTTTTACT TGTGGAGATC CCGATCATCT AATGCGGCAG 10058 |||||||||| || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGTTCCCAG GTTAGAGATT TTGTTTTACT TGTGGAGATC CCGATCATCT AATGCGGCAG 629 TGTACTTCTC AAAGGGGTCG TGGTGGGCCT CGACCAAATT CTTCATTCCA GACTAGACCA 10118 ||||||||| |||||||||| |||||||||| ||||| |||| |||||||||| ||||||||| TGTACTTCTT AAAGGGGTCG TGGTGGGCCT CGACCTAATT CTTCATTCCA TACTAGACCA 689 CCAGCACCAC AGGGTAGAGG TCGTGGCAGA GTTCAGTCAG GTAGAGGTGA TAGAGTTTCC 10178 | ||| | || |||||||||| |||| ||||| || ||||||| |||| ||||| ||||||||| CTAGCTCTAC AGGGTAGAGG TCGTTGCAGA GTACAGTCAG GTAG-GGTGA TAGAGTTTCT 748 AGTAGTGGTG TTGCAGCTCA GCAGAGTGGG GGTAGAGGTA CCACCCA 10225 |||||||||| ||| |||||| ||| ||| || |||||||||| ||||||| AGTAGTGGTG TTGGAGCTCA GCAAAGTTGG GGTAGAGGTA CCACCCA 795 hqPGS_C06HBa0016K14.1-4+_SGN-U339520- (9701 10225) ******************************************************************************** EST sequence 15 +strand 762 n (File: SGN-U329579+) 1 TCAAGTGAAA GCACATGAGA TTCATCCGAA ATAAACTTCC GAAGCATAGA GACATGAAAA 61 ACAGGATGAA CTACAAGCTA CCTCTCCTAC TCGGCTCAAA ATCTCAAAAG GTCCAATGAA 121 TCTAGGGCTA AACTTGCCCT TTTTTCCAAA CCTCATCACA CCCTTCATGG GTGATACTCG 181 GAGCCAAACA TGATCACCCT CCATAAACAC TAAGGCTCTA ATTCCCTGAT CTGCATAACT 241 CTGTCGACTC TGAACTGTCA ATAATCTACT GAATCATACG GACTTGCTAC ATAGCATCTC 301 TAAGCAAGTT TGTATCCAAA GAGTCCATCT TCGCCGAATC AAACCAACCA ATCGGAGACC 361 TACACCGTCT TCCATACAAC GCCTCAAATG GGGCCATATG GATACTAGAG TGATAACTGT 421 TATTATAGCC AAACTCCGCT AAGGATAAAT GTTGATCCCA TCTAGCACCA AAATCGATCA 481 CACACGCTCG AAGCATATCT TCCAATACTT GCATGGTCCG CTCAGATTGA CCATCTGTTT 541 GAGGGTGAAA TGTTGTACTC ATATCTAACT GAGTACACAG ACCATGTTGT AATGCCTTCA 601 AGAATTGAGA TGTAAATAGT GAACCTCGAT CTGATATGAT AAAAATACGA ACTCCATGTA 661 TTCGAACAAT CTGACTGATA TATAGCTCGA CTAACTTTTC TGCCGTATAC TACATCCGAA 721 CCGGAATGAA GTGAGCAGAC TTGGTCAGCC TATCAACATA AC Predicted gene structure (within gDNA segment 14212 to 11019): Exon 1 13513 13439 ( 75 n); cDNA 1 75 ( 75 n); score: 0.907 Intron 1 13438 13409 ( 30 n); Pd: 0.000 (s: 0.90), Pa: 0.000 (s: 0.89) ?? Exon 2 13408 12717 ( 692 n); cDNA 76 762 ( 687 n); score: 0.915 MATCH C06HBa0016K14.1-4- SGN-U329579+ 0.915 767 1.007 C PGS_C06HBa0016K14.1-4-_SGN-U329579+ (13513 13439,13408 12717) Alignment (genomic DNA sequence = upper lines): TCGAGTGAAA TCACATGAGA TTCATCCGGA ATATACTTCC GAAGCATAGA GACATGGAAA 13454 || ||||||| ||||||||| |||||||| | ||| |||||| |||||||||| |||||| ||| TCAAGTGAAA GCACATGAGA TTCATCCGAA ATAAACTTCC GAAGCATAGA GACATGAAAA 60 ACAGGATGAA CTGCCGACAA ACTAGGTGGC AAGGCCAACT TATAG-GCCA CCTCTCCCAC 13395 |||||||||| || | || | ||||||| || ACAGGATGAA CTACA..... .......... .......... .....AGCTA CCTCTCCTAC 90 TCGGCTCAAA ATCTCAAAAG GTCCAATGAA CCTAGGGCTA AGCTTGCCCT TCTTTCCAAA 13335 |||||||||| |||||||||| |||||||||| ||||||||| | |||||||| | |||||||| TCGGCTCAAA ATCTCAAAAG GTCCAATGAA TCTAGGGCTA AACTTGCCCT TTTTTCCAAA 150 CCTCATTACA CCCTTCATGG GTGATACTCG GAGCCAAACA TGATCACCCT CCATAAACAC 13275 |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCTCATCACA CCCTTCATGG GTGATACTCG GAGCCAAACA TGATCACCCT CCATAAACAC 210 CAAGGCTCTA ACTCTCCGGT CTGCATAACT CTTTTGTCGA CTCTGAGCTG TCAATAATCT 13215 ||||||||| | || | | | |||||||||| | |||||| |||||| ||| ||||||||| TAAGGCTCTA ATTCCCTGAT CTGCATAACT C---TGTCGA CTCTGAACTG TCAATAATC- 266 ATACTGAATC ATACGAACTT GCTCCATAGC ATCTCTAAGC AAGTCTGTAT CCAAAGAGTC 13155 ||||||||| ||||| |||| ||| |||||| |||||||||| |||| ||||| |||||||||| -TACTGAATC ATACGGACTT GCTACATAGC ATCTCTAAGC AAGTTTGTAT CCAAAGAGTC 325 CATCTCCGCC GAATCAAACC AACCAATAGG AGATCTACAC CGTCTACCAT ATAATGCCTC 13095 ||||| |||| |||||||||| ||||||| || ||| |||||| ||||| |||| | || ||||| CATCTTCGCC GAATCAAACC AACCAATCGG AGACCTACAC CGTCTTCCAT ACAACGCCTC 385 AAATGGGGCC ATTTGGATAC TAGAGTGATA ACTGTTATTA TAGGCAAACT CCGCTAAGGG 13035 |||||||||| || ||||||| |||||||||| |||||||||| ||| |||||| ||||||||| AAATGGGGCC ATATGGATAC TAGAGTGATA ACTGTTATTA TAGCCAAACT CCGCTAAGGA 445 TAAATGTCGA TCCCATCTTG CACCAAAATC GATCACACAC GCTCGAAGCA TATCTTCCAA 12975 ||||||| || |||||||| | |||||||||| |||||||||| |||||||||| |||||||||| TAAATGTTGA TCCCATCTAG CACCAAAATC GATCACACAC GCTCGAAGCA TATCTTCCAA 505 TACCTGAATG GTCCGCTCAG ATTGACCATC TGTCTGAGGG TGAAATGCCG TACTCATATC 12915 ||| || ||| |||||||||| |||||||||| ||| |||||| ||||||| | |||||||||| TACTTGCATG GTCCGCTCAG ATTGACCATC TGTTTGAGGG TGAAATGTTG TACTCATATC 565 TAACTGAGTA CCCAGACCAT GTTGTAATGC CTTCCAGAAG TGAGAAGTAA ATAGTGAACC 12855 |||||||||| | |||||||| |||||||||| |||| |||| ||||| |||| |||||||||| TAACTGAGTA CACAGACCAT GTTGTAATGC CTTCAAGAAT TGAGATGTAA ATAGTGAACC 625 TCGATCTGAT ATGATAGAAA GAGGAACTCC ATGTAGTCGT ACAATCTGAC TGATATATAG 12795 |||||||||| |||||| ||| | ||||||| ||||| ||| |||||||||| |||||||||| TCGATCTGAT ATGATAAAAA TACGAACTCC ATGTATTCGA ACAATCTGAC TGATATATAG 685 TTCGGCTAAC TTTTCTGCTG TATACTTTAC CCGAACCGGG ATGAAGTGGG CAGACTTGGT 12735 ||| ||||| |||||||| | |||||| | ||||||||| |||||||| | |||||||||| CTCGACTAAC TTTTCTGCCG TATACTACAT CCGAACCGGA ATGAAGTGAG CAGACTTGGT 745 CAGTCTATCA ACAACAAC 12717 ||| |||||| || | ||| CAGCCTATCA AC-ATAAC 762 hqPGS_C06HBa0016K14.1-4-_SGN-U329579+ (13513 13439,13408 12717) ******************************************************************************** EST sequence 1 -strand 897 n (File: SGN-U342484-) 1 AATTTTAACG AGGTCCCCAG ATTATGTGGT AAAGCTTTCC AGAAATAAGA AGTAAATAGT 61 GACCCTCGAT CTGATATGAT AGAAATGGAA ACTCCAGGTA GTGGACCAAT GTGCTGAAAT 121 ATAGCTCAGC TACTTTTTCN ACCGTATACT TCACCGAACC CGGAATGAAG TGGGCAGATT 181 TGGTCAGCCT ATCATCACAA ACCCAAATAG AGTCATAACC ACCCACTGTG GTAGGCAAAC 241 CCACAACAAA GTCCATAGTA ATCCACTCCC ACGTCCAAGT AAGAATAGGC ATCCTTTGAG 301 ATACACCCCC AGGCCACTGG TGCTCACACT TGACCAACTG GCAAGTCAAA CACCTCAAAA 361 CAAAGTCTGT AATATCTCTC TTCATCCCAC ACCACCAGTA ATGCTGACTC AGATCATGAT 421 ACATTTTCGC CTCTCCCGGA TGCATAGAAT ATCGAGAACA ATGGGCCTCC TCAAGGATCA 481 ATCTAATCAA ATCGCCTGTC TTGGGCACAC AAATCCTGCC TCCGATCCTC AAGACACCAT 541 CAGAATCAAG GACAACCTCC TTAGCTTCCC CTCTCAATAC TTTGTCTCGA ATGAGACATA 601 ATTTTTCGTC ATCAAACTGG TGTGCACGAA TCTGCTTGAC TAAAGAAGAT CGAGCCTCAA 661 TAAAAGCAAT CATCCCATCA CTCTCCTCTG AGATCTGTAA TCGGACAAGA CTGTTAGCTA 721 ACAATTGAAC ATCTCTAGCC AATGGTCTTT CCTCAACACT AAGAGATGCA AGACTCCCCA 781 TGCTATGAGT CTTCCTACTC AAAGCATCGG CCACAACATT GGCCTTCCCC GGATGATACA 841 AAATCCTCGT GCCGAATTTC CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCGG Predicted gene structure (within gDNA segment 15319 to 10414): Exon 1 12914 12075 ( 840 n); cDNA 6 844 ( 839 n); score: 0.894 MATCH C06HBa0016K14.1-4- SGN-U342484- 0.894 840 0.936 C PGS_C06HBa0016K14.1-4-_SGN-U342484- (12914 12075) Alignment (genomic DNA sequence = upper lines): TAACTGAGTA CCCAGACCAT GTTGTAATGC CTTCCAGAAG TGAGAAGTAA ATAGTGAACC 12855 |||| || |||||| || || |||| || |||||||| | |||||||| ||||||| || TAACGAGGTC CCCAGATTAT GTGGTAAAGC TTTCCAGAAA TAAGAAGTAA ATAGTGACCC 65 TCGATCTGAT ATGATAGAAA GAGGAACTCC ATGTAGTCGT ACAATCTGAC TGATATATAG 12795 |||||||||| |||||||||| | |||||| | ||||| | |||| || | ||| |||||| TCGATCTGAT ATGATAGAAA TGGAAACTCC AGGTAGTGGA CCAATGTG-C TGAAATATAG 124 TTCGGCTAAC TTTTCTGCTG TATACTTTAC CCGAACCGGG ATGAAGTGGG CAGACTTGGT 12735 || |||| ||||| | | ||||||| || | | |||| |||||||||| |||| ||||| CTCAGCTACT TTTTCNACCG TATACTTCAC CGAACCCGGA ATGAAGTGGG CAGATTTGGT 184 CAGTCTATCA ACAACAACCC AAATAGAGTC ATAACCACCC ACTGTGGTAG GCAAACCCAC 12675 ||| |||||| || ||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGCCTATCA TCACAAACCC AAATAGAGTC ATAACCACCC ACTGTGGTAG GCAAACCCAC 244 AACAAAGTCC ATAGTAATCC GCTCCCACTT CCAAGTAGGA ATAGGCATCC TCTGAGATAC 12615 |||||||||| |||||||||| ||||||| | ||||||| || |||||||||| | |||||||| AACAAAGTCC ATAGTAATCC ACTCCCACGT CCAAGTAAGA ATAGGCATCC TTTGAGATAC 304 ACCCCCTGGC CGCTGGTGCT CACATTTGAC CTGCTGGCAA GTCAAACACC TAGAAACAAA 12555 |||||| ||| | |||||||| |||| ||||| | ||||||| |||||||||| | ||||||| ACCCCCAGGC CACTGGTGCT CACACTTGAC CAACTGGCAA GTCAAACACC TCAAAACAAA 364 GTCTGAAATA TCTCTCTTCA TCCCACACCA CCAGTAATGC TGACTCAGAT CATGATACAT 12495 ||||| |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTGTAATA TCTCTCTTCA TCCCACACCA CCAGTAATGC TGACTCAGAT CATGATACAT 424 CTTTGCCGCT CCCGGATGGA TGGAATACCG AGTACAATGG GCCTCCTCAA GAATCAATCT 12435 || ||| || |||||||| | | ||||| || || ||||||| |||||||||| | |||||||| TTTCGCCTCT CCCGGATGCA TAGAATATCG AGAACAATGG GCCTCCTCAA GGATCAATCT 484 AATCAAATCG CCTGTCCTGG GCACACAAAT TCTGCCTCCG ATCCGCAAGA CACCATCAGA 12375 |||||||||| |||||| ||| |||||||||| ||||||||| |||| ||||| |||||||||| AATCAAATCG CCTGTCTTGG GCACACAAAT CCTGCCTCCG ATCCTCAAGA CACCATCAGA 544 ATCAAGGACA GCCTCCTTAG CTTCCCCTCT CAATACTCTG TCTCGAATGA GACATAATTT 12315 |||||||||| ||||||||| |||||||||| ||||||| || |||||||||| |||||||||| ATCAAGGACA ACCTCCTTAG CTTCCCCTCT CAATACTTTG TCTCGAATGA GACATAATTT 604 TTCGTCATCA AATTGGTGTG CACGAATTTG CTCGACTAAA GAAGACCGAG CCTCAATAAA 12255 |||||||||| || ||||||| ||||||| || || ||||||| ||||| |||| |||||||||| TTCGTCATCA AACTGGTGTG CACGAATCTG CTTGACTAAA GAAGATCGAG CCTCAATAAA 664 AGCAATCATC CCCTCACTCT CTTCTGAAAT CTGCAAGCGG ACAAGACTGT TAGCTAATAT 12195 |||||||||| || ||||||| | ||||| || ||| || ||| |||||||||| ||||||| | AGCAATCATC CCATCACTCT CCTCTGAGAT CTGTAATCGG ACAAGACTGT TAGCTAACAA 724 CTGCACATCT CTAGCCAATG GTCTCTCCAT AATACTAAGT GCTGCAAGAC TCCCCATGCT 12135 || |||||| |||||||||| |||| ||| || |||||| | |||||||| |||||||||| TTGAACATCT CTAGCCAATG GTCTTTCCTC AACACTAAGA GATGCAAGAC TCCCCATGCT 784 AGGAGTCTTC CTACTCAGAG CATCGGCCAT AACATTGGCC TTTCCTGGAT GATACAGAAT 12075 | |||||||| ||||||| || ||||||||| |||||||||| || || |||| |||||| ||| ATGAGTCTTC CTACTCAAAG CATCGGCCAC AACATTGGCC TTCCCCGGAT GATACAAAAT 844 hqPGS_C06HBa0016K14.1-4-_SGN-U342484- (12914 12075) ******************************************************************************** EST sequence 13 -strand 854 n (File: SGN-U339313-) 1 CCTTGTGGAC TATTGAGGCA AGGGATTTTC TAATTCTTCT AAGTGTATGA ATGCGTGTAT 61 GTCCTTGTGT TATATGTTGG AGTACGGGAT GGGGTATGGG TTGACTTGTC CACATTGATT 121 ANTTCTAATG ATGAAAGAAA GGGGATACAA AAGGCAATGT GATTAATGGT TTATGTGTGT 181 TGTGTTGAGA AAGAATCGAA GGCTTGTTGA ATCATTGTTG ATGTAACTTG ATGTTTGTGT 241 GGTTGTGAAC TGTAGGATGT TATGAAAATG GTCATCTCCT CATTATATGT GTGAACTTGT 301 CATCTGCATT ATTCTGAGGC ATTGGTTGTG GCAAGTGTTA TGTGCATTGA GAAATAATGG 361 GTACTTAAGA GGATGTACCA TTTCGAGGGA CGTATCGCGC GCCGCGATGG ATACTATATT 421 TCGAGGAACG TATCGCGCGC CGTGATGGTT ACTATTATCG AGGATCGTGT CGTGCGCCGC 481 GACAGATGCA TGGACAGATA TGTCCCCCAA GGGTCCCGGA CTGAGAGACA GCGGGTGTAT 541 ATCACTAGGT CAGACATGCA TCATTATACT TGACATTGCA TTCCATTGCA TTACACACTT 601 ATCATTAGTG AACTTGATAT CGTATTTTCT CGATCTTCTT ATTGCCTTTC TGCAGAACTT 661 GTGATTGATT AATATTGAGC TTGTTATGGC GGATATATAA TTTGTTGAAC TATTCTTGTT 721 GAGGATATGT AATTTGTTGA AGTGTTGTTG TTGAGGATAT GTAATTTGTC CTCGTGCCGA 781 ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGACC GCCACCGCGG TGGAGCTCCA 841 GCATTGGTTT CAAT Predicted gene structure (within gDNA segment 9748 to 15924): Exon 1 11809 11853 ( 45 n); cDNA 322 366 ( 45 n); score: 0.567 Intron 1 11854 14897 (3044 n); Pd: 0.000 (s: 0.57), Pa: 0.502 (s: 0.86) Exon 2 14898 15255 ( 358 n); cDNA 367 722 ( 356 n); score: 0.804 MATCH C06HBa0016K14.1-4+ SGN-U339313- 0.804 403 0.472 C PGS_C06HBa0016K14.1-4+_SGN-U339313- (11809 11853,14898 15255) Alignment (genomic DNA sequence = upper lines): TTGGTT-TGG GTGGTGTGTT GATGCAGAAG GGGAAAGTGA TTGCTTATGC TTCTAGGCAA 11867 |||||| ||| |||| | | |||| | | | || | ||| TTGGTTGTGG CAAGTGTTAT G-TGCATTGA GAAATAATGG GTACTT.... .......... 366 TTGAAATCCC ATGAGAAAAA CTACCCTACT CATGATTTGG AGTTGGCGGC TGTGGTATTT 11927 .......... .......... .......... .......... .......... .......... 366 GTACTTAAGT TATGGCGTCA TTATTTGTAT GGGGTGCATT GTGAGATCTT CACTGATCAT 11987 .......... .......... .......... .......... .......... .......... 366 CGGAGTCTTC AATATATCTT TAGCTAGAGG GATTTGAACT TGAGGCAACG GAGATGGCTT 12047 .......... .......... .......... .......... .......... .......... 366 GAGTTGTTGA AGGACTATGA TGTGACCATT CTGTATCATC CAGGAAAGGC CAATGTTATG 12107 .......... .......... .......... .......... .......... .......... 366 GCCGATGCTC TGAGTAGGAA GACTCCTAGC ATGGGGAGTC TTGCAGCACT TAGTATTATG 12167 .......... .......... .......... .......... .......... .......... 366 GAGAGACCAT TGGCTAGAGA TGTGCAGATA TTAGCTAACA GTCTTGTCCG CTTGCAGATT 12227 .......... .......... .......... .......... .......... .......... 366 TCAGAAGAGA GTGAGGGGAT GATTGCTTTT ATTGAGGCTC GGTCTTCTTT AGTCGAGCAA 12287 .......... .......... .......... .......... .......... .......... 366 ATTCGTGCAC ACCAATTTGA TGACGAAAAA TTATGTCTCA TTCGAGACAG AGTATTGAGA 12347 .......... .......... .......... .......... .......... .......... 366 GGGGAAGCTA AGGAGGCTGT CCTTGATTCT GATGGTGTCT TGCGGATCGG AGGCAGAATT 12407 .......... .......... .......... .......... .......... .......... 366 TGTGTGCCCA GGACAGGCGA TTTGATTAGA TTGATTCTTG AGGAGGCCCA TTGTACTCGG 12467 .......... .......... .......... .......... .......... .......... 366 TATTCCATCC ATCCGGGAGC GGCAAAGATG TATCATGATC TGAGTCAGCA TTACTGGTGG 12527 .......... .......... .......... .......... .......... .......... 366 TGTGGGATGA AGAGAGATAT TTCAGACTTT GTTTCTAGGT GTTTGACTTG CCAGCAGGTC 12587 .......... .......... .......... .......... .......... .......... 366 AAATGTGAGC ACCAGCGGCC AGGGGGTGTA TCTCAGAGGA TGCCTATTCC TACTTGGAAG 12647 .......... .......... .......... .......... .......... .......... 366 TGGGAGCGGA TTACTATGGA CTTTGTTGTG GGTTTGCCTA CCACAGTGGG TGGTTATGAC 12707 .......... .......... .......... .......... .......... .......... 366 TCTATTTGGG TTGTTGTTGA TAGACTGACC AAGTCTGCCC ACTTCATCCC GGTTCGGGTA 12767 .......... .......... .......... .......... .......... .......... 366 AAGTATACAG CAGAAAAGTT AGCCGAACTA TATATCAGTC AGATTGTACG ACTACATGGA 12827 .......... .......... .......... .......... .......... .......... 366 GTTCCTCTTT CTATCATATC AGATCGAGGT TCACTATTTA CTTCTCACTT CTGGAAGGCA 12887 .......... .......... .......... .......... .......... .......... 366 TTACAACATG GTCTGGGTAC TCAGTTAGAT ATGAGTACGG CATTTCACCC TCAGACAGAT 12947 .......... .......... .......... .......... .......... .......... 366 GGTCAATCTG AGCGGACCAT TCAGGTATTG GAAGATATGC TTCGAGCGTG TGTGATCGAT 13007 .......... .......... .......... .......... .......... .......... 366 TTTGGTGCAA GATGGGATCG ACATTTACCC TTAGCGGAGT TTGCCTATAA TAACAGTTAT 13067 .......... .......... .......... .......... .......... .......... 366 CACTCTAGTA TCCAAATGGC CCCATTTGAG GCATTATATG GTAGACGGTG TAGATCTCCT 13127 .......... .......... .......... .......... .......... .......... 366 ATTGGTTGGT TTGATTCGGC GGAGATGGAC TCTTTGGATA CAGACTTGCT TAGAGATGCT 13187 .......... .......... .......... .......... .......... .......... 366 ATGGAGCAAG TTCGTATGAT TCAGTATAGA TTATTGACAG CTCAGAGTCG ACAAAAGAGT 13247 .......... .......... .......... .......... .......... .......... 366 TATGCAGACC GGAGAGTTAG AGCCTTGGTG TTTATGGAGG GTGATCATGT TTGGCTCCGA 13307 .......... .......... .......... .......... .......... .......... 366 GTATCACCCA TGAAGGGTGT AATGAGGTTT GGAAAGAAGG GCAAGCTTAG CCCTAGGTTC 13367 .......... .......... .......... .......... .......... .......... 366 ATTGGACCTT TTGAGATTTT GAGCCGAGTG GGAGAGGTGG CCTATAAGTT GGCCTTGCCA 13427 .......... .......... .......... .......... .......... .......... 366 CCTAGTTTGT CGGCAGTTCA TCCTGTTTTC CATGTCTCTA TGCTTCGGAA GTATATTCCG 13487 .......... .......... .......... .......... .......... .......... 366 GATGAATCTC ATGTGATTTC ACTCGATTCT GTGGAGCTGG GTCCAGACTT GACATTTGAG 13547 .......... .......... .......... .......... .......... .......... 366 GAGGAGCCTA TAGCTATTTT GGATAGGCAA ATTCGAAAGC TTAGGACCAA AGAGATTGCT 13607 .......... .......... .......... .......... .......... .......... 366 TCAGTGAAGG TGCAATGGAA GCACCGATCA GTGAGAGAGG CAACTTGGGA GACAGAGTCT 13667 .......... .......... .......... .......... .......... .......... 366 GACATGCGTG CCAGATATCC TCAACTTTTT GAAGCATCAG GTACTTTCTT TTACTTTATG 13727 .......... .......... .......... .......... .......... .......... 366 TTCGAGGACG AACATGATTT TTAGTGGTGG ATAATGTAAT GACCCGGAAG GTCATTTTTG 13787 .......... .......... .......... .......... .......... .......... 366 GAAATTTTAA ATATTAGTGT AACTTGAGTT AATTAATCGA TAGTTTATGG GCTAAAATGA 13847 .......... .......... .......... .......... .......... .......... 366 TAATTTATTT AAGACCCTAT ACTATTTAGC CTTTATTTAA TTAAATATGT GGAACAAAAC 13907 .......... .......... .......... .......... .......... .......... 366 TAACCTAAAC GTGTGACGAA AAACAAAGAA GAGGAGAAAG TCTGAAAAAC ACTCGCAGCG 13967 .......... .......... .......... .......... .......... .......... 366 CCGTCGAACA AGTGCACTTG ATTCAGGTAA TAATTTTAAA CTTTGCATTG AGGCTGCTGA 14027 .......... .......... .......... .......... .......... .......... 366 ATATGTCTTG TAATTAATGT ACAATTATAA TTTTTATGTT AACTTTAATA TGTGCTTTGG 14087 .......... .......... .......... .......... .......... .......... 366 AATGTGAAAC ATGGCAAAAT GAATATGGAA TAAAGAAATA ATATTTGGAA GTTGGTATAT 14147 .......... .......... .......... .......... .......... .......... 366 TGGTATGAAG TAATTTTGAA ATTTAGAAAG AGGGTCATGC CAATCATGTG ATAATTGTTG 14207 .......... .......... .......... .......... .......... .......... 366 GGAATATAAT TGCATCAAGA TGATTGAAAA ATAATAGATA GGAATATAGG GTATCCATTT 14267 .......... .......... .......... .......... .......... .......... 366 GAGGATTTAA AATATATTTT TTTGTCCTCG TGATTTTCTC TGTAACTTAA GGCTTCACTG 14327 .......... .......... .......... .......... .......... .......... 366 TTTCTTAGAA TTTTCTTATT ATTATCATAT TAAGATTCAA GTGTGATATA TTGAGATAGG 14387 .......... .......... .......... .......... .......... .......... 366 TAATTAAATA TTAGATATAT TATATTATAT AGATATCGTT AAAGTTATGA TATTTGGAGA 14447 .......... .......... .......... .......... .......... .......... 366 AATTGAAATT TAACCGTAAG CTTAAATTTG GAAAACATGA GAGTTCACAT GTTAATTGAC 14507 .......... .......... .......... .......... .......... .......... 366 ATCAAGAATT ACAAACTTGA TTATAAATTT GGGTGAAGTT TTATGTCAAG GTTAAACACA 14567 .......... .......... .......... .......... .......... .......... 366 TAATAATGTG GGTGTTGTTA GTTCCAAACC TTATTGTGAA TATACATATT TGAATAGATT 14627 .......... .......... .......... .......... .......... .......... 366 GCATCGATTT GGAAGCTCAA CTTAAAGGGA AGGCTCAAGT CCCGTAGTGA TTATTCAAGT 14687 .......... .......... .......... .......... .......... .......... 366 GACTAAGGCA AGTGGATTTC TAAACTCTTG TTAAGCGTAC GAAATTCGTG TATTTCCTTG 14747 .......... .......... .......... .......... .......... .......... 366 TGTGATGTTG AGAATGATTT GGAGACTTGT TGTTTATTTG TTGGTGTTGT ATTTCTTGTT 14807 .......... .......... .......... .......... .......... .......... 366 GTAAATTGTG TTTGGTTATC AAATGGTTAT CTCCTCCCTT TTCATTTGAG CATGTCATTT 14867 .......... .......... .......... .......... .......... .......... 366 GCATTGTATC TGAGACATTG TTGTGGTAAG AGGATGGTGT ACTATTTTCG AGGGTCGTAT 14927 | || | ||| || | ||||| |||| ||||| .......... .......... .......... AAGAGGATGT ACCA-TTTCG AGGGACGTAT 395 CGCGCGCCGC GATGGATATT ATATTTCGAG GGACGTATCG CGCGCCGCGA AGGTTACTAC 14987 |||||||||| |||||||| | |||||||||| | |||||||| ||||||| || |||||||| CGCGCGCCGC GATGGATACT ATATTTCGAG GAACGTATCG CGCGCCGTGA TGGTTACTA- 454 TTATCGAGGG TCGTGTCGTG CGCCGCGACA GATGCATGGA CAGATATGTC CCCCATGGGT 15047 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| |||| TTATCGAGGA TCGTGTCGTG CGCCGCGACA GATGCATGGA CAGATATGTC CCCCAAGGGT 514 CCCGAACTGA GAGACAGCGG GTGTGTATCG TTAGGAAAGA CATGCATCAC GATATTTGAC 15107 |||| ||||| |||||||||| |||| |||| |||| ||| ||||||||| ||| ||||| CCCGGACTGA GAGACAGCGG GTGTATATCA CTAGGTCAGA CATGCATCAT TATACTTGAC 574 ATTGCATCTC ATGACATTGC ACATCTTATT ATTGATGAAC TTGAACATGT GTTTTGCTGA 15167 ||||||| | || |||| | ||| ||||| ||| ||||| |||| || |||| || ATTGCATTCC ATTGCATTAC ACA-CTTATC ATTAGTGAAC TTGATATCGT ATTTTCTCGA 633 TCTTGTGATT GTTTCTCTG- TGAAGTCTGT GATTGTTGAA TATTGAG-TT GATATTGAGA 15225 |||| | ||| | | |||| ||| | ||| ||||| | || ||||||| || | ||| | | TCTTCTTATT GCCTTTCTGC AGAACT-TGT GATTGATTAA TATTGAGCTT GTTATGGCGG 692 ATGTGTAAAC TGATAGAGTG TGGTTATTGA 15255 || | ||| || | | | | || |||| ATATATAATT TGTTGAACTA TTCTTGTTGA 722 hqPGS_C06HBa0016K14.1-4+_SGN-U339313- (14898 15255) ******************************************************************************** EST sequence 4 +strand 681 n (File: SGN-U328762+) 1 CTTGTGGTAT ATGTTTGGGA GTAACGGGAT TGGTGATGGA TTGACTTGTC CACATTGATT 61 AATTTAATGA TGAAAAAAAA AGGATAAAAA AAAAGGCAAT GTGATTAAAT ATTTAAGTGT 121 GATGTGTTCA GAAAGGGATG AAGGATTGTT GAATCATTGT TGATGTGATT TCATGTTTGT 181 GTGGTTTTGA ATTGTGCAAT GTTATGAAAA TGGTCATCTC CTCATTATTT GTGTGAACTT 241 GCCATCTGCA TTATTATGAG GCATTGGTTG TGACAAGTGT TATGTGCATT GAGAAAGAAT 301 GAGTACTTAA GAGGATGTAC AATTTCGAGG GACGTATCGC GCGCCGCGAT GGATACTATA 361 TTTCGAGGGA CGTATCGCAT GCCGCGATGG ATACTATATT TTGAGGGACG TATCGCGCGC 421 CGCGATGGTT ACTATTATCG AGGGTCGTGT CGTGCGCCGC GACAGATGCA TGGACAGATA 481 TGTCCCCCAT GGTTCCCAGA CTGAGAGACA GCGGGTGTGT ATCACTAGGT CAGACATGCA 541 TCATTATACT TGACAATGCA TTACACATAC TTATCATTGG TGAACTTGAT ATTGTGTTTT 601 GCTGATCTTG TGATTGAATT TCTGCGAAAT TTGTGATTGA TGAATACTGA GCTTGTTATT 661 GAGGATATAT AATTTGTTGA A Predicted gene structure (within gDNA segment 11078 to 15924): Exon 1 14900 15237 ( 338 n); cDNA 348 676 ( 329 n); score: 0.840 MATCH C06HBa0016K14.1-4+ SGN-U328762+ 0.840 338 0.496 C PGS_C06HBa0016K14.1-4+_SGN-U328762+ (14900 15237) Alignment (genomic DNA sequence = upper lines): GATGGTGTAC TAT-TTTCGA GGGTCGTATC GCGCGCCGCG ATGGATATTA TATTTCGAGG 14958 ||||| ||| ||| |||||| ||| |||||| || |||||| ||||||| || ||||| |||| GATGG-ATAC TATATTTCGA GGGACGTATC GCATGCCGCG ATGGATACTA TATTTTGAGG 406 GACGTATCGC GCGCCGCGAA GGTTACTACT TATCGAGGGT CGTGTCGTGC GCCGCGACAG 15018 |||||||||| ||||||||| |||||||| | |||||||||| |||||||||| |||||||||| GACGTATCGC GCGCCGCGAT GGTTACTA-T TATCGAGGGT CGTGTCGTGC GCCGCGACAG 465 ATGCATGGAC AGATATGTCC CCCATGGGTC CCGAACTGAG AGACAGCGGG TGTGTATCGT 15078 |||||||||| |||||||||| ||||||| || || |||||| |||||||||| |||||||| ATGCATGGAC AGATATGTCC CCCATGGTTC CCAGACTGAG AGACAGCGGG TGTGTATCAC 525 TAGGAAAGAC ATGCATCACG ATATTTGACA TTGCATCTCA TGACATTGCA CATCTTATTA 15138 |||| |||| |||||||| ||| |||||| |||| | | ||| || | ||||| | TAGGTCAGAC ATGCATCATT ATACTTGACA ATGCA--T-- T-ACA---CA TA-CTTATCA 576 TTGATGAACT TGAACATGTG TTTTGCTGAT CTTGTGATTG TTTCTCTGTG AAGTCTGTGA 15198 ||| |||||| ||| |||| |||||||||| |||||||||| | |||| | || | ||||| TTGGTGAACT TGATATTGTG TTTTGCTGAT CTTGTGATTG AATTTCTGCG AAATTTGTGA 636 TTGTTGAATA TTGAG-TTGA TATTGAGAAT GTGTAAACTG 15237 ||| |||||| |||| ||| ||||||| || | ||| || TTGATGAATA CTGAGCTTGT TATTGAGGAT ATATAATTTG 676 hqPGS_C06HBa0016K14.1-4+_SGN-U328762+ (14900 15237) ******************************************************************************** EST sequence 11 -strand 904 n (File: SGN-U337987-) 1 NACATATCGC GCGCGGCGAT GGATACTATA TTCGAGGGAC GTTCCGCGCG CTGCGATGGT 61 TACTATAATC GAGGGTCGTG TCGTGCGCCG CGACAGATGC ATGGACATAT ATGTCTCCCA 121 TGGGTCCCGG ACTGAGAGAC AGTGGGTGTG TATCACTAGG TCAGACATGC ATCATTATAC 181 TTGACATTGC ATTCCATTGC ATTGCACATA CTTACCATTA GTGAACTTGA TATCGTATTT 241 CGCTGATGTT TTGATTGCTT TTCTGCGGAA CTTGTGATTG ATCAATATTG AGCTTGTTAT 301 TGCGGATATG TAATTTGTTG AAGTATTGTT GTTGAGGATA TGTAATTTGT TGAAGTATTG 361 TTGTTGAGGA TATGTAATTT GTTGAAGTGT TGTTGTTGAG GATATGTAAT TTGCCTAAGT 421 GTTGTTGTTG AGCTATGTGC TATGTAAATT GTGAGCTGTT AGGTTGGGTT GATTTTAATG 481 CAGGTTGTAT TTGTGGAGGT TCGGTTGGGG GTGGTAGGAG TACCCGTATT TCATCCCCTT 541 AGCTTATGTT TAGAGGTTTA CTTGCTGAGT ACCGTGTGGT TTGGTACTCA CCCCTTGCTT 601 CTACAAATTT TTGTAGGTTA TGAGCCTGGA TTTTTGTTGT ACTTGTCAGT CTCTTCTTTT 661 CCGAGGCTTC ATGGAGATTT GTGAGGTAGC TGTTTCCATC TCAGCAGACT TTCTTCTCCA 721 TAATTATGAT CTTGTTCTAT TCTAGAAACA AGTTCATTTG AGACTTGAGT TTTTTTCTTT 781 TGAATCTTGT AATACTTTAG AGGCTTGTAC ACGTGACTAC CCAGGTCTTG GGGGTTTAAT 841 TAAGTTACTT ATATTTTATT TCTGCAAAAA AAAAAAAAAA AAACTCGAGG GGGGGCCCGG 901 TCCC Predicted gene structure (within gDNA segment 13710 to 15924): Exon 1 14925 15255 ( 331 n); cDNA 5 335 ( 331 n); score: 0.813 PPA cDNA 866 884 MATCH C06HBa0016K14.1-4+ SGN-U337987- 0.813 331 0.366 C PGS_C06HBa0016K14.1-4+_SGN-U337987- (14925 15255) Alignment (genomic DNA sequence = upper lines): TATCGCGCGC CGCGATGGAT ATTATATTTC GAGGGACGTA TCGCGCGCCG CGAAGGTTAC 14984 |||||||||| ||||||||| | |||| ||| ||||||||| ||||||| | ||| |||||| TATCGCGCGC GGCGATGGAT ACTATA-TTC GAGGGACGTT CCGCGCGCTG CGATGGTTAC 63 TACTTATCGA GGGTCGTGTC GTGCGCCGCG ACAGATGCAT GGACAGATAT GTCCCCCATG 15044 || | ||||| |||||||||| |||||||||| |||||||||| ||||| |||| ||| |||||| TA-TAATCGA GGGTCGTGTC GTGCGCCGCG ACAGATGCAT GGACATATAT GTCTCCCATG 122 GGTCCCGAAC TGAGAGACAG CGGGTGTGTA TCGTTAGGAA AGACATGCAT CACGATATTT 15104 ||||||| || |||||||||| ||||||||| || |||| |||||||||| || ||| || GGTCCCGGAC TGAGAGACAG TGGGTGTGTA TCACTAGGTC AGACATGCAT CATTATACTT 182 GACATTGCAT CTCATGACAT TGCACAT-CT TATTATTGAT GAACTTGAAC ATGTGTTTTG 15163 |||||||||| ||| ||| ||||||| || || ||| | |||||||| || ||| | GACATTGCAT TCCATTGCAT TGCACATACT TACCATTAGT GAACTTGATA TCGTATTTCG 242 CTGATCTTGT GATTGTTTCT CTGTGAAGTC TGTGATTGTT GAATATTGAG -TTGATATTG 15222 ||||| || | ||||| || | ||| | | |||||||| | ||||||||| ||| ||||| CTGATGTTTT GATTGCTTTT CTGCGGAACT TGTGATTGAT CAATATTGAG CTTGTTATTG 302 AGAATGTGTA AACTGATAGA GTGTGGTTAT TGA 15255 | || |||| | || | | || | ||| | ||| CGGATATGTA ATTTGTTGAA GTATTGTTGT TGA 335 hqPGS_C06HBa0016K14.1-4+_SGN-U337987- (14925 15255) Total number of EST alignments reported: 15 ________________________________________________________________________________ Predicted gene locations (6) in segment 1 to 15924: PGL 1 (+ strand): 1808 5091 AGS-1 (1808 2279,2658 2965,3354 3489,3575 3717,3818 3941,4385 5091) SCR (e 1.000 d 0.992 a 0.943,e 1.000 d 0.944 a 0.981,e 0.993 d 0.964 a 0.967,e 1.000 d 0.846 a 0.970,e 1.000 d 0.999 a 0.969,e 1.000) Exon 1 1808 2279 ( 472 n); score: 1.000 Intron 1 2280 2657 ( 378 n); Pd: 0.992 Pa: 0.943 Exon 2 2658 2965 ( 308 n); score: 1.000 Intron 2 2966 3353 ( 388 n); Pd: 0.944 Pa: 0.981 Exon 3 3354 3489 ( 136 n); score: 0.993 Intron 3 3490 3574 ( 85 n); Pd: 0.964 Pa: 0.967 Exon 4 3575 3717 ( 143 n); score: 1.000 Intron 4 3718 3817 ( 100 n); Pd: 0.846 Pa: 0.970 Exon 5 3818 3941 ( 124 n); score: 1.000 Intron 5 3942 4384 ( 443 n); Pd: 0.999 Pa: 0.969 Exon 6 4385 5091 ( 707 n); score: 1.000 PGS (1808 2279,2658 2965,3354 3489,3575 3717,3818 3941,4385 5091) SGN-U319927+ PGS (1923 2279,2658 2965,3354 3419) SGN-U319928+ PGS (1937 2279,2658 2965) SGN-U336971+ PGS (1953 2279) SGN-U336972+ 3-phase translation of AGS-1 (+strand): . . . . . . 1808 TCTTTCACTACCCTACATTTAACATTTTCCTATAACCACTGCTTTAGATAAGTCAAATTT S F T T L H L T F S Y N H C F R - V K F L S L P Y I - H F P I T T A L D K S N L F H Y P T F N I F L - P L L - I S Q I . . . . . . 1868 AGCTCTTTGTTTTGATCTCTGTTTCAAAAGAAAACACCTATTAAGCAGCCATCATCTTTC S S L F - S L F Q K K T P I K Q P S S F A L C F D L C F K R K H L L S S H H L S - L F V L I S V S K E N T Y - A A I I F . . . . . . 1928 TTATCTTTTCCAAAACCAAAACTACTGACTTTTCTTGAAAAAAGAAGAGGTGGGGTGCTT L S F P K P K L L T F L E K R R G G V L Y L F Q N Q N Y - L F L K K E E V G C F L I F S K T K T T D F S - K K K R W G A . . . . . . 1988 TCTTTTCTTCAAAAACCTTCTCTTTGTTCTTGAAAAAACAGGACTCATTCATTTTTTTTT S F L Q K P S L C S - K N R T H S F F F L F F K N L L F V L E K T G L I H F F L F F S S K T F S L F L K K Q D S F I F F . . . . . . 2048 GTGTGTTTCTTTCAGAAGAAATAACAAAGACCCTTTCTCTGTTTTCTTCATATTTCAGCT V C F F Q K K - Q R P F L C F L H I S A C V S F R R N N K D P F S V F F I F Q L C V F L S E E I T K T L S L F S S Y F S . . . . . . 2108 TTGAGCTACTTGGATCTGTTTTTTTTTTTTTGAATATACAAGTAGTTTGTGTGTTCTGGG L S Y L D L F F F F - I Y K - F V C S G - A T W I C F F F F E Y T S S L C V L G F E L L G S V F F F L N I Q V V C V F W . . . . . . 2168 GTCTACAGAAGAAGGAGAAGCTAAAAGGGGTGATTTTGTTTTTTGTTTGTTGTTGTTCTA V Y R R R R S - K G - F C F L F V V V L S T E E G E A K R G D F V F C L L L F - G L Q K K E K L K G V I L F F V C C C S . . . . . . : 2228 ATGGGGATATGTTTGAGTGCTAGAATTAAAGCTGAAAGCCCATTTCATACAG : GGCTAAAT M G I C L S A R I K A E S P F H T : G L N W G Y V - V L E L K L K A H F I Q : G - I N G D M F E C - N - S - K P I S Y R : A K . . . . . . 2666 TCAAGATCTGTTAGCATAGATGGTGGTGATAGTAATTCAAGCTCCAGGGTTCCATCAACT S R S V S I D G G D S N S S S R V P S T Q D L L A - M V V I V I Q A P G F H Q L F K I C - H R W W - - - F K L Q G S I N . . . . . . 2726 ACTAGAAGTGAAGGAGAGATATTGCAGTCTCCCAATTTGAAGAGCTTTAGTTTTTCTGAT T R S E G E I L Q S P N L K S F S F S D L E V K E R Y C S L P I - R A L V F L I Y - K - R R D I A V S Q F E E L - F F - . . . . . . 2786 CTCAGAACAGCCACTAGGAACTTTCGTCCCGATAGTGTGCTGGGAGAAGGTGGATTTGGA L R T A T R N F R P D S V L G E G G F G S E Q P L G T F V P I V C W E K V D L D S Q N S H - E L S S R - C A G R R W I W . . . . . . 2846 TCAGTTTTTAAAGGGTGGATCGATGAGAACACGTTCGCAGCTACCAAGCCTGGGACAGGT S V F K G W I D E N T F A A T K P G T G Q F L K G G S M R T R S Q L P S L G Q V I S F - R V D R - E H V R S Y Q A W D R . . . . . . : 2906 GTGATCATTGCTGTGAAAAGGCTTAATCAAGAAGGTTTTCAAGGTCACAGGGAATGGCTG : V I I A V K R L N Q E G F Q G H R E W L : - S L L - K G L I K K V F K V T G N G W : C D H C C E K A - S R R F S R S Q G M A : . . . . . . 3354 GCAGAAGTTAACTATTTGGGGCAGTTTTCTCATCCTCATCTTGTAAAGCTGATTGGCTAC A E V N Y L G Q F S H P H L V K L I G Y Q K L T I W G S F L I L I L - S - L A T G R S - L F G A V F S S S S C K A D W L . . . . . . 3414 TGCTTGGAGGACGAACATCGTCTTTTGGTTTATGAGTTCATGCCTCGGGGCAGCTTGGAA C L E D E H R L L V Y E F M P R G S L E A W R T N I V F W F M S S C L G A A W K L L G G R T S S F G L - V H A S G Q L G . . : . . . . 3474 AACCATTTGTTTAGGA : GAGGTTCTTATTTCCAGCCTCTTTCTTGGAAACTACGCTTGAAG N H L F R : R G S Y F Q P L S W K L R L K T I C L G : E V L I S S L F L G N Y A - R K P F V - E : R F L F P A S F L E T T L E . . . . . . 3619 GTTGCTCTAGGAGCTGCAAAAGGGCTTGCTTTTCTCCACAGTGCTGAGACTAAAGTGATA V A L G A A K G L A F L H S A E T K V I L L - E L Q K G L L F S T V L R L K - Y G C S R S C K R A C F S P Q C - D - S D . . . . : . . 3679 TATCGAGACTTCAAAACCTCGAATATTTTGCTTGATTCG : AACTACACTGCCAAACTCTCA Y R D F K T S N I L L D S : N Y T A K L S I E T S K P R I F C L I R : T T L P N S Q I S R L Q N L E Y F A - F : E L H C Q T L . . . . . . 3839 GACTTTGGATTAGCCAAGGATGGGCCAACAGGCGATAAAAGCCATGTCTCTACAAGGGTT D F G L A K D G P T G D K S H V S T R V T L D - P R M G Q Q A I K A M S L Q G L R L W I S Q G W A N R R - K P C L Y K G . . . . . : . 3899 ATGGGGACCTATGGATATGCCGCTCCAGAATACATGGCTACTG : GTCATTTAACTAGCAAG M G T Y G Y A A P E Y M A T : G H L T S K W G P M D M P L Q N T W L L : V I - L A R Y G D L W I C R S R I H G Y W : S F N - Q . . . . . . 4402 AGTGATGTGTATAGTTTTGGAGTTGTCCTCCTTGAAATGCTGTCAGGTCGTAGGGCAATC S D V Y S F G V V L L E M L S G R R A I V M C I V L E L S S L K C C Q V V G Q S E - C V - F W S C P P - N A V R S - G N . . . . . . 4462 GACAAGAATAGACCATCCGGAGAACACAATCTAGTGGAGTGGGCGAAACCTTACCTAGGT D K N R P S G E H N L V E W A K P Y L G T R I D H P E N T I - W S G R N L T - V R Q E - T I R R T Q S S G V G E T L P R . . . . . . 4522 AACAAACGTAAGGTCTTCCGTGTTCTGGATACCCGTCTTGAAGGCCAGTATTCAATGGAG N K R K V F R V L D T R L E G Q Y S M E T N V R S S V F W I P V L K A S I Q W R - Q T - G L P C S G Y P S - R P V F N G . . . . . . 4582 GTGGCGAGTAAAGTAGCTAACCTTGCCCTGCGGTGCCTATCCAAAGATCCTCGGTTTAGA V A S K V A N L A L R C L S K D P R F R W R V K - L T L P C G A Y P K I L G L D G G E - S S - P C P A V P I Q R S S V - . . . . . . 4642 CCAAGCATGAGTGACATTGTTAAAGAACTGGAGCAACTTTATCAACAATCTAAAGATTCA P S M S D I V K E L E Q L Y Q Q S K D S Q A - V T L L K N W S N F I N N L K I Q T K H E - H C - R T G A T L S T I - R F . . . . . . 4702 GGAAATACTCGCAGCCACGCTAGCAACCGACCTAGACCACGTAGACGAAGTGCTGGTGAT G N T R S H A S N R P R P R R R S A G D E I L A A T L A T D L D H V D E V L V M R K Y S Q P R - Q P T - T T - T K C W - . . . . . . 4762 GTTGCTAATAGAAATCCTTCAGTTGCTTATCCGAGACCGTCTGCTTCTCCCCTTTACGCT V A N R N P S V A Y P R P S A S P L Y A L L I E I L Q L L I R D R L L L P F T L C C - - K S F S C L S E T V C F S P L R . . . . . . 4822 AAATAATTAAACAAATGATTGAAAGATGCCTTAATTTCTTGTTTTTTTTGTCTTATATGT K - L N K - L K D A L I S C F F C L I C N N - T N D - K M P - F L V F F V L Y V - I I K Q M I E R C L N F L F F L S Y M . . . . . . 4882 GTTTATCAAGCTTGATAAGTAGTTGAGGCTGATGAAGAACCTCTGCACATTTGCAGTATC V Y Q A - - V V E A D E E P L H I C S I F I K L D K - L R L M K N L C T F A V S C L S S L I S S - G - - R T S A H L Q Y . . . . . . 4942 AGCTGGCTGATTGTACAGTTGTTTTTGGCTAATGTATTGAATCTATACTTTCTGTAGGTC S W L I V Q L F L A N V L N L Y F L - V A G - L Y S C F W L M Y - I Y T F C R S Q L A D C T V V F G - C I E S I L S V G . . . . . . 5002 CTGAATGTGTTTGTAACTTTGAGTTTTGCTCACAGCTCACTATATGTTCACTTTTTGACA L N V F V T L S F A H S S L Y V H F L T - M C L - L - V L L T A H Y M F T F - H P E C V C N F E F C S Q L T I C S L F D . . . 5062 CTAAAGGAATGCATACTACCCTTTCATACT L K E C I L P F H T - R N A Y Y P F I T K G M H T T L S Y Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4+_PGL-1_AGS-1_PPS_1 (2201 2279,2658 2965,3354 3489,3575 3717,3818 3941,4385 4827) (frame '1'; 1230 bp, 410 residues) 1 FCFLFVVVLM GICLSARIKA ESPFHTGLNS RSVSIDGGDS NSSSRVPSTT RSEGEILQSP 61 NLKSFSFSDL RTATRNFRPD SVLGEGGFGS VFKGWIDENT FAATKPGTGV IIAVKRLNQE 121 GFQGHREWLA EVNYLGQFSH PHLVKLIGYC LEDEHRLLVY EFMPRGSLEN HLFRRGSYFQ 181 PLSWKLRLKV ALGAAKGLAF LHSAETKVIY RDFKTSNILL DSNYTAKLSD FGLAKDGPTG 241 DKSHVSTRVM GTYGYAAPEY MATGHLTSKS DVYSFGVVLL EMLSGRRAID KNRPSGEHNL 301 VEWAKPYLGN KRKVFRVLDT RLEGQYSMEV ASKVANLALR CLSKDPRFRP SMSDIVKELE 361 QLYQQSKDSG NTRSHASNRP RPRRRSAGDV ANRNPSVAYP RPSASPLYAK - PGL 2 (+ strand): 7664 8030 AGS-1 (7664 8030) SCR (e 0.814) Exon 1 7664 8030 ( 367 n); score: 0.814 PGS (7664 8008) SGN-U339313- PGS (7666 8003) SGN-U328762+ PGS (7691 8030) SGN-U337987- 3-phase translation of AGS-1 (+strand): . . . . . . 7664 AGGATGGTGTACTATTTTCGAGGGTCGTATCGCGCGCCGCGATGGATATTATATTTCGAG R M V Y Y F R G S Y R A P R W I L Y F E G W C T I F E G R I A R R D G Y Y I S R D G V L F S R V V S R A A M D I I F R . . . . . . 7724 GGACGTATCGCGCGCCGCGAAGGTTACTACTTATCGAGGGTCGTGTCGTGCGCCGCGACA G R I A R R E G Y Y L S R V V S C A A T D V S R A A K V T T Y R G S C R A P R Q G T Y R A P R R L L L I E G R V V R R D . . . . . . 7784 GATGCATGGACAGATATGTCCCCCATGGGTCCCGAACTGAGAGACAGCGGGTGTGTATCG D A W T D M S P M G P E L R D S G C V S M H G Q I C P P W V P N - E T A G V Y R R C M D R Y V P H G S R T E R Q R V C I . . . . . . 7844 TTAGGAAAGACATGCATCACGATATTTGACATTGCATCTCATGACATTGCACATCTTATT L G K T C I T I F D I A S H D I A H L I - E R H A S R Y L T L H L M T L H I L L V R K D M H H D I - H C I S - H C T S Y . . . . . . 7904 ATTGATGAACTTGAACATGTGTTTTGCTGATCTTGTGATTGTTTCTCTGTGAAGTCTGTG I D E L E H V F C - S C D C F S V K S V L M N L N M C F A D L V I V S L - S L - Y - - T - T C V L L I L - L F L C E V C . . . . . . 7964 ATTGTTGAATATTGAGTTGATATTGAGAATGTGTAAACTGATAGAGTGTGGTTATTGAGT I V E Y - V D I E N V - T D R V W L L S L L N I E L I L R M C K L I E C G Y - V D C - I L S - Y - E C V N - - S V V I E . 8024 GGTGTGT G V V C W C Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4+_PGL-2_AGS-1_PPS_1 (7664 7933) (frame '1'; 267 bp, 89 residues) 1 RMVYYFRGSY RAPRWILYFE GRIARREGYY LSRVVSCAAT DAWTDMSPMG PELRDSGCVS 61 LGKTCITIFD IASHDIAHLI IDELEHVFC- 3-phase translation of AGS-1 (-strand): . . . . . . 8030 ACACACCACTCAATAACCACACTCTATCAGTTTACACATTCTCAATATCAACTCAATATT T H H S I T T L Y Q F T H S Q Y Q L N I H T T Q - P H S I S L H I L N I N S I F T P L N N H T L S V Y T F S I S T Q Y . . . . . . 7970 CAACAATCACAGACTTCACAGAGAAACAATCACAAGATCAGCAAAACACATGTTCAAGTT Q Q S Q T S Q R N N H K I S K T H V Q V N N H R L H R E T I T R S A K H M F K F S T I T D F T E K Q S Q D Q Q N T C S S . . . . . . 7910 CATCAATAATAAGATGTGCAATGTCATGAGATGCAATGTCAAATATCGTGATGCATGTCT H Q - - D V Q C H E M Q C Q I S - C M S I N N K M C N V M R C N V K Y R D A C L S S I I R C A M S - D A M S N I V M H V . . . . . . 7850 TTCCTAACGATACACACCCGCTGTCTCTCAGTTCGGGACCCATGGGGGACATATCTGTCC F L T I H T R C L S V R D P W G T Y L S S - R Y T P A V S Q F G T H G G H I C P F P N D T H P L S L S S G P M G D I S V . . . . . . 7790 ATGCATCTGTCGCGGCGCACGACACGACCCTCGATAAGTAGTAACCTTCGCGGCGCGCGA M H L S R R T T R P S I S S N L R G A R C I C R G A R H D P R - V V T F A A R D H A S V A A H D T T L D K - - P S R R A . . . . . . 7730 TACGTCCCTCGAAATATAATATCCATCGCGGCGCGCGATACGACCCTCGAAAATAGTACA Y V P R N I I S I A A R D T T L E N S T T S L E I - Y P S R R A I R P S K I V H I R P S K Y N I H R G A R Y D P R K - Y . 7670 CCATCCT P S H P T I Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4-_PGL-2_AGS-1_PPS_1 (7859 7665) (frame '1'; 195 bp, 65 residues) 1 CMSFLTIHTR CLSVRDPWGT YLSMHLSRRT TRPSISSNLR GARYVPRNII SIAARDTTLE 61 NSTPS PGL 3 (+ strand): 8792 9098 AGS-1 (8792 9098) SCR (e 0.919) Exon 1 8792 9098 ( 307 n); score: 0.919 PGS (8792 9098) SGN-U344270- 3-phase translation of AGS-1 (+strand): . . . . . . 8792 CGAGGCAGTTATTGAGGCCCCAGCTAGAGGTAGGGGTAGATCTCGAGCTAGAGGTCGTGC R G S Y - G P S - R - G - I S S - R S C E A V I E A P A R G R G R S R A R G R A R Q L L R P Q L E V G V D L E L E V V . . . . . . 8852 TAGTAGTGCGACAGTAACCAGAGGTCGTGGACGTGGAGCAGCACCAGTGAGAGGTCGTGC - - C D S N Q R S W T W S S T S E R S C S S A T V T R G R G R G A A P V R G R A L V V R Q - P E V V D V E Q H Q - E V V . . . . . . 8912 TAGAGAGGTCTCTACCGAACCACAGATTGATGACAGAGAGGACCAGGTTCCTCCAGATCC - R G L Y R T T D - - Q R G P G S S R S R E V S T E P Q I D D R E D Q V P P D P L E R S L P N H R L M T E R T R F L Q I . . . . . . 8972 CGTAGTCACACCTTTGCTTCAGGATACACTATTGAGGGTGTTAAGTGTGCTAGAGGGCTT R S H T F A S G Y T I E G V K C A R G L V V T P L L Q D T L L R V L S V L E G F P - S H L C F R I H Y - G C - V C - R A . . . . . . 9032 TTCTCAGGGTGGTGGTGCGACTACCACACCACATGACTCTCGTACTAGAGAGGGGGCTCA F S G W W C D Y H T T - L S Y - R G G S S Q G G G A T T T P H D S R T R E G A Q F L R V V V R L P H H M T L V L E R G L . 9092 GACCCAA D P T Q R P Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4+_PGL-3_AGS-1_PPS_1 (8793 9098) (frame '2'; 306 bp, 102 residues) 1 EAVIEAPARG RGRSRARGRA SSATVTRGRG RGAAPVRGRA REVSTEPQID DREDQVPPDP 61 VVTPLLQDTL LRVLSVLEGF SQGGGATTTP HDSRTREGAQ TQ 3-phase translation of AGS-1 (-strand): . . . . . . 9098 TTGGGTCTGAGCCCCCTCTCTAGTACGAGAGTCATGTGGTGTGGTAGTCGCACCACCACC L G L S P L S S T R V M W C G S R T T T W V - A P S L V R E S C G V V V A P P P G S E P P L - Y E S H V V W - S H H H . . . . . . 9038 CTGAGAAAAGCCCTCTAGCACACTTAACACCCTCAATAGTGTATCCTGAAGCAAAGGTGT L R K A L - H T - H P Q - C I L K Q R C - E K P S S T L N T L N S V S - S K G V P E K S P L A H L T P S I V Y P E A K V . . . . . . 8978 GACTACGGGATCTGGAGGAACCTGGTCCTCTCTGTCATCAATCTGTGGTTCGGTAGAGAC D Y G I W R N L V L S V I N L W F G R D T T G S G G T W S S L S S I C G S V E T - L R D L E E P G P L C H Q S V V R - R . . . . . . 8918 CTCTCTAGCACGACCTCTCACTGGTGCTGCTCCACGTCCACGACCTCTGGTTACTGTCGC L S S T T S H W C C S T S T T S G Y C R S L A R P L T G A A P R P R P L V T V A P L - H D L S L V L L H V H D L W L L S . . . . . . 8858 ACTACTAGCACGACCTCTAGCTCGAGATCTACCCCTACCTCTAGCTGGGGCCTCAATAAC T T S T T S S S R S T P T S S W G L N N L L A R P L A R D L P L P L A G A S I T H Y - H D L - L E I Y P Y L - L G P Q - . 8798 TGCCTCG C L A S L P Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4-_PGL-3_AGS-1_PPS_1 (8999 8793) (frame '1'; 207 bp, 69 residues) 1 CILKQRCDYG IWRNLVLSVI NLWFGRDLSS TTSHWCCSTS TTSGYCRTTS TTSSSRSTPT 61 SSWGLNNCL >C06HBa0016K14.1-4-_PGL-3_AGS-1_PPS_2 (8989 8792) (frame '2'; 198 bp, 66 residues) 1 SKGVTTGSGG TWSSLSSICG SVETSLARPL TGAAPRPRPL VTVALLARPL ARDLPLPLAG 61 ASITAS PGL 4 (+ strand): 9526 10225 AGS-1 (9526 10225) SCR (e 0.896) Exon 1 9526 10225 ( 700 n); score: 0.896 PGS (9526 10081) SGN-U339521+ PGS (9701 10225) SGN-U339520- 3-phase translation of AGS-1 (+strand): . . . . . . 9526 TTGAGAGTCTGAGACAGGATGGTTTATCGGTTACAGAGTATGAGGCGCGTTTTTGCCAGT L R V - D R M V Y R L Q S M R R V F A S - E S E T G W F I G Y R V - G A F L P V E S L R Q D G L S V T E Y E A R F C Q . . . . . . 9586 TGTCTAGGCATGCGTTGGCCATTATTCCAAATGAGACAGAGAGGATCCGCAGATTCGTGA C L G M R W P L F Q M R Q R G S A D S - V - A C V G H Y S K - D R E D P Q I R E L S R H A L A I I P N E T E R I R R F V . . . . . . 9646 GGGGATTGACTTTCTCTATCAGGTCAGCTGTATTTCGGACATCTAGGGAGGGGACTTCTT G D - L S L S G Q L Y F G H L G R G L L G I D F L Y Q V S C I S D I - G G D F F R G L T F S I R S A V F R T S R E G T S . . . . . . 9706 TCCAGTCCATTGTGAGCGCCGCCAAAGAGGCGGAATTGATGGAGAGAGAAGAGTTTGGGG S S P L - A P P K R R N - W R E K S L G P V H C E R R Q R G G I D G E R R V W G F Q S I V S A A K E A E L M E R E E F G . . . . . . 9766 ACCCTAAAAGAGCTCGTATATCAGGCCAGTTTCAGGGTGCCTCATCTGGAGGTAGGGGAT T L K E L V Y Q A S F R V P H L E V G D P - K S S Y I R P V S G C L I W R - G I D P K R A R I S G Q F Q G A S S G G R G . . . . . . 9826 CACAGAGAGTGAGTGGTTCTTTTCAGCAGCGGGGACCTATTCATGCATCTATGCCGACAT H R E - V V L F S S G D L F M H L C R H T E S E W F F S A A G T Y S C I Y A D I S Q R V S G S F Q Q R G P I H A S M P T . . . . . . 9886 TTGAGGGTGGCCAGACATCTAGGGGTTCTTATGGCCCAGGTCAGGGCTCGTACGGTTCAC L R V A R H L G V L M A Q V R A R T V H - G W P D I - G F L W P R S G L V R F T F E G G Q T S R G S Y G P G Q G S Y G S . . . . . . 9946 AACAGCGACCTACAGGGCGAGGAAATTATAGTGGGTTTTCAGGGTCCACACAACAGTTCC N S D L Q G E E I I V G F Q G P H N S S T A T Y R A R K L - W V F R V H T T V P Q Q R P T G R G N Y S G F S G S T Q Q F . . . . . . 10006 CAGGTCAGAGATTTTGTTTTACTTGTGGAGATCCCGATCATCTAATGCGGCAGTGTACTT Q V R D F V L L V E I P I I - C G S V L R S E I L F Y L W R S R S S N A A V Y F P G Q R F C F T C G D P D H L M R Q C T . . . . . . 10066 CTCAAAGGGGTCGTGGTGGGCCTCGACCAAATTCTTCATTCCAGACTAGACCACCAGCAC L K G V V V G L D Q I L H S R L D H Q H S K G S W W A S T K F F I P D - T T S T S Q R G R G G P R P N S S F Q T R P P A . . . . . . 10126 CACAGGGTAGAGGTCGTGGCAGAGTTCAGTCAGGTAGAGGTGATAGAGTTTCCAGTAGTG H R V E V V A E F S Q V E V I E F P V V T G - R S W Q S S V R - R - - S F Q - W P Q G R G R G R V Q S G R G D R V S S S . . . . 10186 GTGTTGCAGCTCAGCAGAGTGGGGGTAGAGGTACCACCCA V L Q L S R V G V E V P P C C S S A E W G - R Y H P G V A A Q Q S G G R G T T Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4+_PGL-4_AGS-1_PPS_1 (9528 10223) (frame '0'; 696 bp, 232 residues) 1 ESLRQDGLSV TEYEARFCQL SRHALAIIPN ETERIRRFVR GLTFSIRSAV FRTSREGTSF 61 QSIVSAAKEA ELMEREEFGD PKRARISGQF QGASSGGRGS QRVSGSFQQR GPIHASMPTF 121 EGGQTSRGSY GPGQGSYGSQ QRPTGRGNYS GFSGSTQQFP GQRFCFTCGD PDHLMRQCTS 181 QRGRGGPRPN SSFQTRPPAP QGRGRGRVQS GRGDRVSSSG VAAQQSGGRG TT 3-phase translation of AGS-1 (-strand): . . . . . . 10225 TGGGTGGTACCTCTACCCCCACTCTGCTGAGCTGCAACACCACTACTGGAAACTCTATCA W V V P L P P L C - A A T P L L E T L S G W Y L Y P H S A E L Q H H Y W K L Y H G G T S T P T L L S C N T T T G N S I . . . . . . 10165 CCTCTACCTGACTGAACTCTGCCACGACCTCTACCCTGTGGTGCTGGTGGTCTAGTCTGG P L P D - T L P R P L P C G A G G L V W L Y L T E L C H D L Y P V V L V V - S G T S T - L N S A T T S T L W C W W S S L . . . . . . 10105 AATGAAGAATTTGGTCGAGGCCCACCACGACCCCTTTGAGAAGTACACTGCCGCATTAGA N E E F G R G P P R P L - E V H C R I R M K N L V E A H H D P F E K Y T A A L D E - R I W S R P T T T P L R S T L P H - . . . . . . 10045 TGATCGGGATCTCCACAAGTAAAACAAAATCTCTGACCTGGGAACTGTTGTGTGGACCCT - S G S P Q V K Q N L - P G N C C V D P D R D L H K - N K I S D L G T V V W T L M I G I S T S K T K S L T W E L L C G P . . . . . . 9985 GAAAACCCACTATAATTTCCTCGCCCTGTAGGTCGCTGTTGTGAACCGTACGAGCCCTGA E N P L - F P R P V G R C C E P Y E P - K T H Y N F L A L - V A V V N R T S P D - K P T I I S S P C R S L L - T V R A L . . . . . . 9925 CCTGGGCCATAAGAACCCCTAGATGTCTGGCCACCCTCAAATGTCGGCATAGATGCATGA P G P - E P L D V W P P S N V G I D A - L G H K N P - M S G H P Q M S A - M H E T W A I R T P R C L A T L K C R H R C M . . . . . . 9865 ATAGGTCCCCGCTGCTGAAAAGAACCACTCACTCTCTGTGATCCCCTACCTCCAGATGAG I G P R C - K E P L T L C D P L P P D E - V P A A E K N H S L S V I P Y L Q M R N R S P L L K R T T H S L - S P T S R - . . . . . . 9805 GCACCCTGAAACTGGCCTGATATACGAGCTCTTTTAGGGTCCCCAAACTCTTCTCTCTCC A P - N W P D I R A L L G S P N S S L S H P E T G L I Y E L F - G P Q T L L S P G T L K L A - Y T S S F R V P K L F S L . . . . . . 9745 ATCAATTCCGCCTCTTTGGCGGCGCTCACAATGGACTGGAAAGAAGTCCCCTCCCTAGAT I N S A S L A A L T M D W K E V P S L D S I P P L W R R S Q W T G K K S P P - M H Q F R L F G G A H N G L E R S P L P R . . . . . . 9685 GTCCGAAATACAGCTGACCTGATAGAGAAAGTCAATCCCCTCACGAATCTGCGGATCCTC V R N T A D L I E K V N P L T N L R I L S E I Q L T - - R K S I P S R I C G S S C P K Y S - P D R E S Q S P H E S A D P . . . . . . 9625 TCTGTCTCATTTGGAATAATGGCCAACGCATGCCTAGACAACTGGCAAAAACGCGCCTCA S V S F G I M A N A C L D N W Q K R A S L S H L E - W P T H A - T T G K N A P H L C L I W N N G Q R M P R Q L A K T R L . . . . 9565 TACTCTGTAACCGATAAACCATCCTGTCTCAGACTCTCAA Y S V T D K P S C L R L S T L - P I N H P V S D S Q I L C N R - T I L S Q T L Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4-_PGL-4_AGS-1_PPS_1 (9796 9527) (frame '1'; 270 bp, 90 residues) 1 NWPDIRALLG SPNSSLSINS ASLAALTMDW KEVPSLDVRN TADLIEKVNP LTNLRILSVS 61 FGIMANACLD NWQKRASYSV TDKPSCLRLS PGL 5 (- strand): 13513 12075 AGS-1 (13513 13439,13408 12075) SCR (e 0.907 d 0.000 a 0.000,e 0.915) Exon 1 13513 13439 ( 75 n); score: 0.907 Intron 1 13438 13409 ( 30 n); Pd: 0.000 Pa: 0.000 Exon 2 13408 12075 (1334 n); score: 0.915 PGS (12914 12075) SGN-U342484- PGS (13513 13439,13408 12717) SGN-U329579+ 3-phase translation of AGS-1 (-strand): . . . . . . 13513 TCGAGTGAAATCACATGAGATTCATCCGGAATATACTTCCGAAGCATAGAGACATGGAAA S S E I T - D S S G I Y F R S I E T W K R V K S H E I H P E Y T S E A - R H G K E - N H M R F I R N I L P K H R D M E . . : . . . . 13453 ACAGGATGAACTGCC : GCCACCTCTCCCACTCGGCTCAAAATCTCAAAAGGTCCAATGAAC T G - T A : A T S P T R L K I S K G P M N Q D E L P : P P L P L G S K S Q K V Q - T N R M N C : R H L S H S A Q N L K R S N E . . . . . . 13363 CTAGGGCTAAGCTTGCCCTTCTTTCCAAACCTCATTACACCCTTCATGGGTGATACTCGG L G L S L P F F P N L I T P F M G D T R - G - A C P S F Q T S L H P S W V I L G P R A K L A L L S K P H Y T L H G - Y S . . . . . . 13303 AGCCAAACATGATCACCCTCCATAAACACCAAGGCTCTAACTCTCCGGTCTGCATAACTC S Q T - S P S I N T K A L T L R S A - L A K H D H P P - T P R L - L S G L H N S E P N M I T L H K H Q G S N S P V C I T . . . . . . 13243 TTTTGTCGACTCTGAGCTGTCAATAATCTATACTGAATCATACGAACTTGCTCCATAGCA F C R L - A V N N L Y - I I R T C S I A F V D S E L S I I Y T E S Y E L A P - H L L S T L S C Q - S I L N H T N L L H S . . . . . . 13183 TCTCTAAGCAAGTCTGTATCCAAAGAGTCCATCTCCGCCGAATCAAACCAACCAATAGGA S L S K S V S K E S I S A E S N Q P I G L - A S L Y P K S P S P P N Q T N Q - E I S K Q V C I Q R V H L R R I K P T N R . . . . . . 13123 GATCTACACCGTCTACCATATAATGCCTCAAATGGGGCCATTTGGATACTAGAGTGATAA D L H R L P Y N A S N G A I W I L E - - I Y T V Y H I M P Q M G P F G Y - S D N R S T P S T I - C L K W G H L D T R V I . . . . . . 13063 CTGTTATTATAGGCAAACTCCGCTAAGGGTAAATGTCGATCCCATCTTGCACCAAAATCG L L L - A N S A K G K C R S H L A P K S C Y Y R Q T P L R V N V D P I L H Q N R T V I I G K L R - G - M S I P S C T K I . . . . . . 13003 ATCACACACGCTCGAAGCATATCTTCCAATACCTGAATGGTCCGCTCAGATTGACCATCT I T H A R S I S S N T - M V R S D - P S S H T L E A Y L P I P E W S A Q I D H L D H T R S K H I F Q Y L N G P L R L T I . . . . . . 12943 GTCTGAGGGTGAAATGCCGTACTCATATCTAACTGAGTACCCAGACCATGTTGTAATGCC V - G - N A V L I S N - V P R P C C N A S E G E M P Y S Y L T E Y P D H V V M P C L R V K C R T H I - L S T Q T M L - C . . . . . . 12883 TTCCAGAAGTGAGAAGTAAATAGTGAACCTCGATCTGATATGATAGAAAGAGGAACTCCA F Q K - E V N S E P R S D M I E R G T P S R S E K - I V N L D L I - - K E E L H L P E V R S K - - T S I - Y D R K R N S . . . . . . 12823 TGTAGTCGTACAATCTGACTGATATATAGTTCGGCTAACTTTTCTGCTGTATACTTTACC C S R T I - L I Y S S A N F S A V Y F T V V V Q S D - Y I V R L T F L L Y T L P M - S Y N L T D I - F G - L F C C I L Y . . . . . . 12763 CGAACCGGGATGAAGTGGGCAGACTTGGTCAGTCTATCAACAACAACCCAAATAGAGTCA R T G M K W A D L V S L S T T T Q I E S E P G - S G Q T W S V Y Q Q Q P K - S H P N R D E V G R L G Q S I N N N P N R V . . . . . . 12703 TAACCACCCACTGTGGTAGGCAAACCCACAACAAAGTCCATAGTAATCCGCTCCCACTTC - P P T V V G K P T T K S I V I R S H F N H P L W - A N P Q Q S P - - S A P T S I T T H C G R Q T H N K V H S N P L P L . . . . . . 12643 CAAGTAGGAATAGGCATCCTCTGAGATACACCCCCTGGCCGCTGGTGCTCACATTTGACC Q V G I G I L - D T P P G R W C S H L T K - E - A S S E I H P L A A G A H I - P P S R N R H P L R Y T P W P L V L T F D . . . . . . 12583 TGCTGGCAAGTCAAACACCTAGAAACAAAGTCTGAAATATCTCTCTTCATCCCACACCAC C W Q V K H L E T K S E I S L F I P H H A G K S N T - K Q S L K Y L S S S H T T L L A S Q T P R N K V - N I S L H P T P . . . . . . 12523 CAGTAATGCTGACTCAGATCATGATACATCTTTGCCGCTCCCGGATGGATGGAATACCGA Q - C - L R S - Y I F A A P G W M E Y R S N A D S D H D T S L P L P D G W N T E P V M L T Q I M I H L C R S R M D G I P . . . . . . 12463 GTACAATGGGCCTCCTCAAGAATCAATCTAATCAAATCGCCTGTCCTGGGCACACAAATT V Q W A S S R I N L I K S P V L G T Q I Y N G P P Q E S I - S N R L S W A H K F S T M G L L K N Q S N Q I A C P G H T N . . . . . . 12403 CTGCCTCCGATCCGCAAGACACCATCAGAATCAAGGACAGCCTCCTTAGCTTCCCCTCTC L P P I R K T P S E S R T A S L A S P L C L R S A R H H Q N Q G Q P P - L P L S S A S D P Q D T I R I K D S L L S F P S . . . . . . 12343 AATACTCTGTCTCGAATGAGACATAATTTTTCGTCATCAAATTGGTGTGCACGAATTTGC N T L S R M R H N F S S S N W C A R I C I L C L E - D I I F R H Q I G V H E F A Q Y S V S N E T - F F V I K L V C T N L . . . . . . 12283 TCGACTAAAGAAGACCGAGCCTCAATAAAAGCAATCATCCCCTCACTCTCTTCTGAAATC S T K E D R A S I K A I I P S L S S E I R L K K T E P Q - K Q S S P H S L L K S L D - R R P S L N K S N H P L T L F - N . . . . . . 12223 TGCAAGCGGACAAGACTGTTAGCTAATATCTGCACATCTCTAGCCAATGGTCTCTCCATA C K R T R L L A N I C T S L A N G L S I A S G Q D C - L I S A H L - P M V S P - L Q A D K T V S - Y L H I S S Q W S L H . . . . . . 12163 ATACTAAGTGCTGCAAGACTCCCCATGCTAGGAGTCTTCCTACTCAGAGCATCGGCCATA I L S A A R L P M L G V F L L R A S A I Y - V L Q D S P C - E S S Y S E H R P - N T K C C K T P H A R S L P T Q S I G H . . . 12103 ACATTGGCCTTTCCTGGATGATACAGAAT T L A F P G - Y R H W P F L D D T E N I G L S W M I Q N Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4-_PGL-5_AGS-1_PPS_1 (12499 12083) (frame '1'; 414 bp, 138 residues) 1 YIFAAPGWME YRVQWASSRI NLIKSPVLGT QILPPIRKTP SESRTASLAS PLNTLSRMRH 61 NFSSSNWCAR ICSTKEDRAS IKAIIPSLSS EICKRTRLLA NICTSLANGL SIILSAARLP 121 MLGVFLLRAS AITLAFPG- >C06HBa0016K14.1-4-_PGL-5_AGS-1_PPS_2 (12785 12549) (frame '0'; 234 bp, 78 residues) 1 LFCCILYPNR DEVGRLGQSI NNNPNRVITT HCGRQTHNKV HSNPLPLPSR NRHPLRYTPW 61 PLVLTFDLLA SQTPRNKV- >C06HBa0016K14.1-4-_PGL-5_AGS-1_PPS_3 (13071 12865) (frame '2'; 204 bp, 68 residues) 1 SDNCYYRQTP LRVNVDPILH QNRSHTLEAY LPIPEWSAQI DHLSEGEMPY SYLTEYPDHV 61 VMPSRSEK- PGL 6 (+ strand): 14898 15255 AGS-1 (14898 15255) SCR (e 0.813) Exon 1 14898 15255 ( 358 n); score: 0.813 PGS (14898 15255) SGN-U339313- PGS (14900 15237) SGN-U328762+ PGS (14925 15255) SGN-U337987- 3-phase translation of AGS-1 (+strand): . . . . . . 14898 AGGATGGTGTACTATTTTCGAGGGTCGTATCGCGCGCCGCGATGGATATTATATTTCGAG R M V Y Y F R G S Y R A P R W I L Y F E G W C T I F E G R I A R R D G Y Y I S R D G V L F S R V V S R A A M D I I F R . . . . . . 14958 GGACGTATCGCGCGCCGCGAAGGTTACTACTTATCGAGGGTCGTGTCGTGCGCCGCGACA G R I A R R E G Y Y L S R V V S C A A T D V S R A A K V T T Y R G S C R A P R Q G T Y R A P R R L L L I E G R V V R R D . . . . . . 15018 GATGCATGGACAGATATGTCCCCCATGGGTCCCGAACTGAGAGACAGCGGGTGTGTATCG D A W T D M S P M G P E L R D S G C V S M H G Q I C P P W V P N - E T A G V Y R R C M D R Y V P H G S R T E R Q R V C I . . . . . . 15078 TTAGGAAAGACATGCATCACGATATTTGACATTGCATCTCATGACATTGCACATCTTATT L G K T C I T I F D I A S H D I A H L I - E R H A S R Y L T L H L M T L H I L L V R K D M H H D I - H C I S - H C T S Y . . . . . . 15138 ATTGATGAACTTGAACATGTGTTTTGCTGATCTTGTGATTGTTTCTCTGTGAAGTCTGTG I D E L E H V F C - S C D C F S V K S V L M N L N M C F A D L V I V S L - S L - Y - - T - T C V L L I L - L F L C E V C . . . . . . 15198 ATTGTTGAATATTGAGTTGATATTGAGAATGTGTAAACTGATAGAGTGTGGTTATTGA I V E Y - V D I E N V - T D R V W L L L L N I E L I L R M C K L I E C G Y - D C - I L S - Y - E C V N - - S V V I Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4+_PGL-6_AGS-1_PPS_1 (14898 15167) (frame '1'; 267 bp, 89 residues) 1 RMVYYFRGSY RAPRWILYFE GRIARREGYY LSRVVSCAAT DAWTDMSPMG PELRDSGCVS 61 LGKTCITIFD IASHDIAHLI IDELEHVFC- 3-phase translation of AGS-1 (-strand): . . . . . . 15255 TCAATAACCACACTCTATCAGTTTACACATTCTCAATATCAACTCAATATTCAACAATCA S I T T L Y Q F T H S Q Y Q L N I Q Q S Q - P H S I S L H I L N I N S I F N N H N N H T L S V Y T F S I S T Q Y S T I . . . . . . 15195 CAGACTTCACAGAGAAACAATCACAAGATCAGCAAAACACATGTTCAAGTTCATCAATAA Q T S Q R N N H K I S K T H V Q V H Q - R L H R E T I T R S A K H M F K F I N N T D F T E K Q S Q D Q Q N T C S S S S I . . . . . . 15135 TAAGATGTGCAATGTCATGAGATGCAATGTCAAATATCGTGATGCATGTCTTTCCTAACG - D V Q C H E M Q C Q I S - C M S F L T K M C N V M R C N V K Y R D A C L S - R I R C A M S - D A M S N I V M H V F P N . . . . . . 15075 ATACACACCCGCTGTCTCTCAGTTCGGGACCCATGGGGGACATATCTGTCCATGCATCTG I H T R C L S V R D P W G T Y L S M H L Y T P A V S Q F G T H G G H I C P C I C D T H P L S L S S G P M G D I S V H A S . . . . . . 15015 TCGCGGCGCACGACACGACCCTCGATAAGTAGTAACCTTCGCGGCGCGCGATACGTCCCT S R R T T R P S I S S N L R G A R Y V P R G A R H D P R - V V T F A A R D T S L V A A H D T T L D K - - P S R R A I R P . . . . . . 14955 CGAAATATAATATCCATCGCGGCGCGCGATACGACCCTCGAAAATAGTACACCATCCT R N I I S I A A R D T T L E N S T P S E I - Y P S R R A I R P S K I V H H P S K Y N I H R G A R Y D P R K - Y T I Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0016K14.1-4-_PGL-6_AGS-1_PPS_1 (15093 14899) (frame '1'; 195 bp, 65 residues) 1 CMSFLTIHTR CLSVRDPWGT YLSMHLSRRT TRPSISSNLR GARYVPRNII SIAARDTTLE 61 NSTPS ... finished at: Mon Aug 28 21:41:49 2006 ________________________________________________________________________________ Sequence 5: C06HBa0016K14.1-5, from 1 to 2745, both strands analyzed. ... started at: Mon Aug 28 21:41:49 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 1 ... matches indexed, elapsed seconds = 1 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 0 No significant EST matches were found. ... finished at: Mon Aug 28 21:41:52 2006 ________________________________________________________________________________ Sequence 6: C06HBa0016K14.1-6, from 1 to 3099, both strands analyzed. ... started at: Mon Aug 28 21:41:52 2006 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 1 ... matches indexed, elapsed seconds = 1 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-XHInCs/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 0 No significant EST matches were found. ... finished at: Mon Aug 28 21:41:55 2006