GeneSeqer. Version of March 12, 2006. Date run: Mon Aug 28 21:15:14 2006 (Bayesian) Splice site model (species): Arabidopsis thaliana Fast search parameters: MinMatchLen 16, MinQualityHSP 30, MinQualityCHAIN 50. Total number of ESTs: 175665 Total sequence length: 93213537 Minimum sequence length: 89 Maximum sequence length: 1082 Length distribution (number of sequences of specified length): < 100: 1 < 200: 2188 < 300: 8544 < 400: 20465 < 500: 39499 < 600: 49432 < 700: 32872 < 800: 19308 < 900: 3155 < 1000: 193 >=1000: 8 Input file : /tmp/bac-submission-temp-rL2EY/C06HBa0046G10/C06HBa0046G10.seq.screen ________________________________________________________________________________ Sequence 1: C06HBa0046G10.1-1, from 1 to 22710, both strands analyzed. ... started at: Mon Aug 28 21:40:17 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 5 HitsTableSize = 5 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 11 ******************************************************************************** EST sequence 15 +strand 647 n (File: SGN-E320889+) 1 TCTAATGTTG AGATTGATTT GTCACTTCCA ATTTCAACTT ATGTGCGAGG TATTCTTGGT 61 TTTAATTCTA TTAACCGTGA TGATTTTGAA GAATATCTTA ATCAATATTT AGAAATATTG 121 GAAATCAAAG ATGGCAACAA AGATTCATTA GGATGGTGGA GTAGGCGAAC CGATGCATTT 181 CCAACTTTGA GCAAAATGGT TCGTGATGTT CTATCTATTC AAGCTTCATC AGTTGCATCA 241 GAGGCTGCTC TCAGTGCGGC AAGATTTCAA ATTAGTTATC ATAGACATTC ATTGGCGGAA 301 GACAGTTTGG AAACAACAGT TTTATTTAGA GACTGGTCCA ATGTCGGAAG GAGGAATAAC 361 AATTTGCCAA AGTTAAGTAA AAAACAAGCA AGTTGGATCA AAACACTAAT TGAATGCAGT 421 GATTATGAAT TAGAGACACA AGAATTTCTA GCAAGTTTGC TAACACCGGA GGATATCACC 481 ATCGATATGA TGCGACAATT AGATAAATTT TAAAGGAGAA AACAAATATT TTTAGATTAC 541 ATTCGAGAAC TAATTGAAAC AGAACCCTTC TAGAAGGTGG CTGCGTANNA TTTAGTACTC 601 ATCTAATATC TGTAACAATA TTAGTTTTAC GTATAAAACT TATTGAA Predicted gene structure (within gDNA segment 16046 to 12048): Exon 1 13888 13593 ( 296 n); cDNA 1 296 ( 296 n); score: 0.856 Intron 1 13592 13520 ( 73 n); Pd: 0.000 (s: 0.84), Pa: 0.972 (s: 0) Exon 2 13519 13492 ( 28 n); cDNA 297 322 ( 26 n); score: 0.643 MATCH C06HBa0046G10.1-1- SGN-E320889+ 0.856 324 0.501 C PGS_C06HBa0046G10.1-1-_SGN-E320889+ (13888 13593,13519 13492) Alignment (genomic DNA sequence = upper lines): TCTAATCAAG ATATTGATTT TGAAGTTCGA ATTTCAACTT ATATGAGAGG TTTTCTTTGT 13829 |||||| | | |||||||| | ||| | |||||||||| || || |||| | ||||| || TCTAATGTTG AGATTGATTT GTCACTTCCA ATTTCAACTT ATGTGCGAGG TATTCTTGGT 60 CTTAATTCTA CTAACCGTGA TGATTTAGAA GAATATATTA ATCAATCTTT AGAAGTATTT 13769 ||||||||| ||||||||| |||||| ||| |||||| ||| |||||| ||| |||| |||| TTTAATTCTA TTAACCGTGA TGATTTTGAA GAATATCTTA ATCAATATTT AGAAATATTG 120 GAAATCAAAG ATGGCAATGA AGATTTATTA GGAT-GTGTA GTAGGCGAAC CGTTGCATTT 13710 |||||||||| ||||||| | ||||| |||| |||| ||| | |||||||||| || ||||||| GAAATCAAAG ATGGCAACAA AGATTCATTA GGATGGTGGA GTAGGCGAAC CGATGCATTT 180 CCAACTTTGA GCAAAATGAT TTGTGATGTT CTAGTTATTC AAGCTTCATC AGTTGCATTG 13650 |||||||||| |||||||| | | |||||||| ||| ||||| |||||||||| |||||||| CCAACTTTGA GCAAAATGGT TCGTGATGTT CTATCTATTC AAGCTTCATC AGTTGCATCA 240 GATGCTGCTT TCAGTGCGGC AAGGTTTCAA ATTGGTGATC ATCGTCATTC AATTTGCATT 13590 || |||||| |||||||||| ||| |||||| ||| || ||| || | ||||| ||| || GAGGCTGCTC TCAGTGCGGC AAGATTTCAA ATTAGTTATC ATAGACATTC -ATTGGC... 296 TTGATCATAA ACAACAATTT TCGAATTGGT AAATACAAGC AAGTTGGATC AAAATGCAAA 13530 .......... .......... .......... .......... .......... .......... 296 TTGAATGCAG TGATGATAAA TTAGAGTCAC AAGAGTTT 13492 || || | || | | || || ||||| .......... GGAAGA-CAG TTTG-GAAAC AACAGTTT 322 hqPGS_C06HBa0046G10.1-1-_SGN-E320889+ (13888 13593,13519 13492) ******************************************************************************** EST sequence 4 -strand 353 n (File: SGN-E392585-) 1 TTGCAGTGAT GATGAATTAG AGGCACAAGA ATTTCTAACA AGTTTGCCAA CACCGGAGGA 61 CATCACCATC GATATGATGC GACAATTAGA ATTAAATTTT AAAGGAGAAA AAAGATATTA 121 TTAGATTACA TTCGAGAACT AATTGAAACA GAACCCTTCC TAGAAGGTGG CTGCGTAAAA 181 TTAGTTACTC ATCTAATATC TGTAACAAAT ATTAGTTTTA CGAATGAGAA CTTATTGAAA 241 CAGAACCCTT CCTAGAAGGT GGCTGCGTAG ATTAGTTACT TTACTAATAT TTGTTAATTG 301 TAAGTTGTAA CTTATAAGTT CTATTGAATA AATTTTTTTA TACAAAAAAA AAA Predicted gene structure (within gDNA segment 14477 to 12419): Exon 1 13524 13217 ( 308 n); cDNA 2 310 ( 309 n); score: 0.911 PPA cDNA 344 353 MATCH C06HBa0046G10.1-1- SGN-E392585- 0.911 308 0.873 C PGS_C06HBa0046G10.1-1-_SGN-E392585- (13524 13217) Alignment (genomic DNA sequence = upper lines): TGCAGTGATG ATAAATTAGA GTCACAAGAG TTTCGGGCAA GTTTGCCAAC ACCGAAGGAT 13465 |||||||||| || ||||||| | ||||||| |||| ||| |||||||||| |||| |||| TGCAGTGATG ATGAATTAGA GGCACAAGAA TTTCTAACAA GTTTGCCAAC ACCGGAGGAC 61 ATCACCATCG ATATGATGCG ACAATTAGAA TTAAATTTTA AAGGAGAAAA AAGATATT-T 13406 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| | ATCACCATCG ATATGATGCG ACAATTAGAA TTAAATTTTA AAGGAGAAAA AAGATATTAT 121 TAGATTACAT AAGAGAACTA ATTGAAAAAA AACCCTTCCT AGAAGGTGGC TGCGTAAAAT 13346 |||||||||| |||||||| ||||||| | |||||||||| |||||||||| |||||||||| TAGATTACAT TCGAGAACTA ATTGAAACAG AACCCTTCCT AGAAGGTGGC TGCGTAAAAT 181 TAGTTACTCA CCTAATTTTT TTAACAGATA TTAGTTTTAA GTATTAAAAC TTATTGAAAC 13286 |||||||||| ||||| | | ||||| ||| ||||||||| | || | ||| |||||||||| TAGTTACTCA TCTAATATCT GTAACAAATA TTAGTTTTAC GAATGAGAAC TTATTGAAAC 241 AGAACCCTTC CTAGAAGGTG GCTGCGTAGA TTAGTTACTC CACTAATATT TGTTAATTGT 13226 |||||||||| |||||||||| |||||||||| ||||||||| ||||||||| |||||||||| AGAACCCTTC CTAGAAGGTG GCTGCGTAGA TTAGTTACTT TACTAATATT TGTTAATTGT 301 AAATTCTAA 13217 || || ||| AAGTTGTAA 310 hqPGS_C06HBa0046G10.1-1-_SGN-E392585- (13524 13217) ******************************************************************************** EST sequence 3 -strand 305 n (File: SGN-E392580-) 1 AACACAGGAG GACATCACCT TCGATATGAT GCGACAATTA GAATTAAATT TTAAAGGAGA 61 AAAAAGATAT TATTAGATTA CATTCGAGAA CTAATTGAAA CAGAACCCTT CCTAGAAGGT 121 GGCTGCGTAA AATTAGTTAC TCATTTAATA TCTGTAACAA ATATTAGTTT TACGAATGAG 181 AACTTATTGA AACAGAACCC TTCCTAGAAG GTGGCTGCGT AGATTAGTTA CTTTACTAAT 241 ATTTGTTAAT TGTAAGTTGT AACTTATAAG TTCTATTGAA TAAATTTTTT TATACAAAAA 301 AAAAA Predicted gene structure (within gDNA segment 14267 to 12419): Exon 1 13477 13217 ( 261 n); cDNA 1 262 ( 262 n); score: 0.906 PPA cDNA 296 305 MATCH C06HBa0046G10.1-1- SGN-E392580- 0.906 261 0.856 C PGS_C06HBa0046G10.1-1-_SGN-E392580- (13477 13217) Alignment (genomic DNA sequence = upper lines): AACACCGAAG GATATCACCA TCGATATGAT GCGACAATTA GAATTAAATT TTAAAGGAGA 13418 ||||| | || || |||||| |||||||||| |||||||||| |||||||||| |||||||||| AACACAGGAG GACATCACCT TCGATATGAT GCGACAATTA GAATTAAATT TTAAAGGAGA 60 AAAAAGATAT T-TTAGATTA CATAAGAGAA CTAATTGAAA AAAAACCCTT CCTAGAAGGT 13359 |||||||||| | |||||||| ||| ||||| |||||||||| | ||||||| |||||||||| AAAAAGATAT TATTAGATTA CATTCGAGAA CTAATTGAAA CAGAACCCTT CCTAGAAGGT 120 GGCTGCGTAA AATTAGTTAC TCACCTAATT TTTTTAACAG ATATTAGTTT TAAGTATTAA 13299 |||||||||| |||||||||| ||| |||| | | ||||| |||||||||| || | || | GGCTGCGTAA AATTAGTTAC TCATTTAATA TCTGTAACAA ATATTAGTTT TACGAATGAG 180 AACTTATTGA AACAGAACCC TTCCTAGAAG GTGGCTGCGT AGATTAGTTA CTCCACTAAT 13239 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || |||||| AACTTATTGA AACAGAACCC TTCCTAGAAG GTGGCTGCGT AGATTAGTTA CTTTACTAAT 240 ATTTGTTAAT TGTAAATTCT AA 13217 |||||||||| ||||| || | || ATTTGTTAAT TGTAAGTTGT AA 262 hqPGS_C06HBa0046G10.1-1-_SGN-E392580- (13477 13217) ******************************************************************************** EST sequence 6 +strand 602 n (File: SGN-E251178+) 1 TCAATTCAAT TTTCTAAATA TAAAAAAATA GATCACTTTA GAGTTGCAAT TGAAGGTATG 61 ATTGAAAAAT TTAAAAAATA TTTTTTTCCT ATTCCTCAAG TTTTTTTAAC GGCTACTTTA 121 CTTCATCCTG CTTATAAATT ACAAGGTGTG AAAGGCCTTG TCGACACTTT TTATGAAACT 181 TTAGAAGTTT TACCGGAAGA AACTCCAGAT TGTGAAACGT GTAAGTATAG CATAAAAATA 241 GAAGCAAAAA TTTTGTATGA AAAATATAGA ACTAGTGAAA ATTTTGAAGG AGAAGTTGCT 301 CAAACTTCTA ATGTTGAGAT TGATTTGTCA GTTCCAATTT CATGTTATAT GCGAGGTTTT 361 CTTGGTCTTA ATTCTACTAA CCGTGATGAT TTTGAAGAAT ATCTTAATCA ATCTTTAGAA 421 AACTTGGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT GGTGGAGTAG ACGAAGCGAT 481 GCATTTCCAA CTTTGAGCAA AATGGTTCGT GATATTCTAG CTATTCAAGC TTCATCAGTT 541 GCATCAGAGG CTGCTTTCAA TGCGGCAAGG TTTCAAATCG GTGATCATAG ACATTCATTG 601 GC Predicted gene structure (within gDNA segment 14804 to 12768): Exon 1 14194 13593 ( 602 n); cDNA 2 602 ( 601 n); score: 0.893 MATCH C06HBa0046G10.1-1- SGN-E251178+ 0.893 602 1.000 C PGS_C06HBa0046G10.1-1-_SGN-E251178+ (14194 13593) Alignment (genomic DNA sequence = upper lines): CAATTCAATT TTCTAAATAT AAAAAAATAG ATAAATTTAA AGTTGCAATT GAAGGTATGA 14135 |||||||||| |||||||||| |||||||||| || | |||| |||||||||| |||||||||| CAATTCAATT TTCTAAATAT AAAAAAATAG ATCACTTTAG AGTTGCAATT GAAGGTATGA 61 TTGAAAAATT AAAAAAATAT TTTTTTCTTA TTCCTCAAAT TTATTTAACT GCTACTTTAC 14075 |||||||||| ||||||||| ||||||| || |||||||| | || |||||| |||||||||| TTGAAAAATT TAAAAAATAT TTTTTTCCTA TTCCTCAAGT TTTTTTAACG GCTACTTTAC 121 TTTCATCCTG ATTATAAATT ACGAGGTGTG CAGGGCCTTA TTGACACTTT TTGTGAAACT 14015 ||||||||| ||||||||| || ||||||| | |||||| | |||||||| || ||||||| -TTCATCCTG CTTATAAATT ACAAGGTGTG AAAGGCCTTG TCGACACTTT TTATGAAACT 180 TAAGAAATTT TACCTAAAGA AATTCCAAAT TGTGAAACGT GTAAGTCTAG CATAAAAGTA 13955 | |||| ||| |||| |||| || |||| || |||||||||| |||||| ||| ||||||| || TTAGAAGTTT TACCGGAAGA AACTCCAGAT TGTGAAACGT GTAAGTATAG CATAAAAATA 240 GAAGCAAAAA TTTTGTATAA AAAATATAGA ACTAGTGAAA ATATTGAAGG AGAAGTTGGT 13895 |||||||||| |||||||| | |||||||||| |||||||||| || ||||||| |||||||| | GAAGCAAAAA TTTTGTATGA AAAATATAGA ACTAGTGAAA ATTTTGAAGG AGAAGTTGCT 300 CAAACTTCTA ATCAAGATAT TGATTTTGAA GTTCGAATTT CAACTTATAT GAGAGGTTTT 13835 |||||||||| || || || |||||| | |||| ||||| || |||||| | |||||||| CAAACTTCTA ATGTTGAGAT TGATTTGTCA GTTCCAATTT CATGTTATAT GCGAGGTTTT 360 CTTTGTCTTA ATTCTACTAA CCGTGATGAT TTAGAAGAAT ATATTAATCA ATCTTTAGAA 13775 ||| |||||| |||||||||| |||||||||| || ||||||| || ||||||| |||||||||| CTTGGTCTTA ATTCTACTAA CCGTGATGAT TTTGAAGAAT ATCTTAATCA ATCTTTAGAA 420 GTATTTGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT -GTGTAGTAG GCGAACCGTT 13716 || |||| |||||||||| |||||||||| |||||||||| ||| ||||| |||| || | AACTTGGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT GGTGGAGTAG ACGAAGCGAT 480 GCATTTCCAA CTTTGAGCAA AATGATTTGT GATGTTCTAG TTATTCAAGC TTCATCAGTT 13656 |||||||||| |||||||||| |||| || || ||| |||||| ||||||||| |||||||||| GCATTTCCAA CTTTGAGCAA AATGGTTCGT GATATTCTAG CTATTCAAGC TTCATCAGTT 540 GCATTGGATG CTGCTTTCAG TGCGGCAAGG TTTCAAATTG GTGATCATCG TCATTCAATT 13596 |||| || | ||||||||| |||||||||| |||||||| | |||||||| | ||||| ||| GCATCAGAGG CTGCTTTCAA TGCGGCAAGG TTTCAAATCG GTGATCATAG ACATTC-ATT 599 TGC 13593 || GGC 602 hqPGS_C06HBa0046G10.1-1-_SGN-E251178+ (14194 13593) ******************************************************************************** EST sequence 8 +strand 625 n (File: SGN-E262684+) 1 TATTATCCTA CTATTTCATC CGTATTAATA AATATTTGTG CTCTTTCAAT TCAATTTTCT 61 AAATATAAAA AAATAGATCA CTTTAGAGTT GCAATTGAAG GTATGATTGA AAAATTTAAA 121 AAATATTTTT TTCCTATTCC TCAAGTTTTT TTAACGGCTA CTTTACTTCA TCCTGCTTAT 181 AAATTACAAG GTGTGAAAGG CCTTGTCGAC ACTTTTTATG AAACTTTAGA AGTTTTACCG 241 GAAGAAACTC CAGATTGTGA AACGTGTAAG TATAGCATAA AAATAGAAGC AAAAATTTTG 301 TATGAAAAAT ATAGAACTAG TGAAAATTTT GAAGGAGAAG TTGCTCAAAC TTCTAATGTT 361 GAGATTGATT TGTCAGTTCC AATTTCATGT TATATGCGAG GTTTTCTTGG TCTTAATTCT 421 ACTAACCGTG ATGATTTTGA AGAATATCTT AATCAATCTT TAGAAAACTT GGAAATCAAA 481 GATGGCAATG AAGATTTATT AGGATGGTGG AGTAGACGAA GCGATGCATT TCCAACTTTG 541 AGCAAAATGG TTCGTGATAT TCTAGCTATT CAAGCTTCAT CAGTTGCATC AGAGGCTGCT 601 TTCAGTGCGG CAAGGTTTCA AATCG Predicted gene structure (within gDNA segment 14840 to 12988): Exon 1 14240 13618 ( 623 n); cDNA 1 623 ( 623 n); score: 0.901 MATCH C06HBa0046G10.1-1- SGN-E262684+ 0.901 623 0.997 C PGS_C06HBa0046G10.1-1-_SGN-E262684+ (14240 13618) Alignment (genomic DNA sequence = upper lines): TATTATCCTA CTATTTTATC AGTATTAATA AATATTTGTG CTCTTACAAT TCAATTTTCT 14181 |||||||||| |||||| ||| ||||||||| |||||||||| ||||| |||| |||||||||| TATTATCCTA CTATTTCATC CGTATTAATA AATATTTGTG CTCTTTCAAT TCAATTTTCT 60 AAATATAAAA AAATAGATAA ATTTAAAGTT GCAATTGAAG GTATGATTGA AAAATTAAAA 14121 |||||||||| |||||||| | |||| |||| |||||||||| |||||||||| |||||| ||| AAATATAAAA AAATAGATCA CTTTAGAGTT GCAATTGAAG GTATGATTGA AAAATTTAAA 120 AAATATTTTT TTCTTATTCC TCAAATTTAT TTAACTGCTA CTTTACTTTC ATCCTGATTA 14061 |||||||||| ||| |||||| |||| ||| | ||||| |||| |||||| ||| |||||| ||| AAATATTTTT TTCCTATTCC TCAAGTTTTT TTAACGGCTA CTTTAC-TTC ATCCTGCTTA 179 TAAATTACGA GGTGTGCAGG GCCTTATTGA CACTTTTTGT GAAACTTAAG AAATTTTACC 14001 |||||||| | |||||| | | ||||| | || |||||||| | ||||||| || || ||||||| TAAATTACAA GGTGTGAAAG GCCTTGTCGA CACTTTTTAT GAAACTTTAG AAGTTTTACC 239 TAAAGAAATT CCAAATTGTG AAACGTGTAA GTCTAGCATA AAAGTAGAAG CAAAAATTTT 13941 |||||| | ||| |||||| |||||||||| || ||||||| ||| |||||| |||||||||| GGAAGAAACT CCAGATTGTG AAACGTGTAA GTATAGCATA AAAATAGAAG CAAAAATTTT 299 GTATAAAAAA TATAGAACTA GTGAAAATAT TGAAGGAGAA GTTGGTCAAA CTTCTAATCA 13881 |||| ||||| |||||||||| |||||||| | |||||||||| |||| ||||| |||||||| GTATGAAAAA TATAGAACTA GTGAAAATTT TGAAGGAGAA GTTGCTCAAA CTTCTAATGT 359 AGATATTGAT TTTGAAGTTC GAATTTCAAC TTATATGAGA GGTTTTCTTT GTCTTAATTC 13821 || |||||| || ||||| ||||||| ||||||| || ||||||||| |||||||||| TGAGATTGAT TTGTCAGTTC CAATTTCATG TTATATGCGA GGTTTTCTTG GTCTTAATTC 419 TACTAACCGT GATGATTTAG AAGAATATAT TAATCAATCT TTAGAAGTAT TTGAAATCAA 13761 |||||||||| |||||||| | |||||||| | |||||||||| |||||| | | |||||||| TACTAACCGT GATGATTTTG AAGAATATCT TAATCAATCT TTAGAAAACT TGGAAATCAA 479 AGATGGCAAT GAAGATTTAT TAGGAT-GTG TAGTAGGCGA ACCGTTGCAT TTCCAACTTT 13702 |||||||||| |||||||||| |||||| ||| ||||| ||| | || ||||| |||||||||| AGATGGCAAT GAAGATTTAT TAGGATGGTG GAGTAGACGA AGCGATGCAT TTCCAACTTT 539 GAGCAAAATG ATTTGTGATG TTCTAGTTAT TCAAGCTTCA TCAGTTGCAT TGGATGCTGC 13642 |||||||||| || ||||| |||||| ||| |||||||||| |||||||||| || ||||| GAGCAAAATG GTTCGTGATA TTCTAGCTAT TCAAGCTTCA TCAGTTGCAT CAGAGGCTGC 599 TTTCAGTGCG GCAAGGTTTC AAAT 13618 |||||||||| |||||||||| |||| TTTCAGTGCG GCAAGGTTTC AAAT 623 hqPGS_C06HBa0046G10.1-1-_SGN-E262684+ (14240 13618) ******************************************************************************** EST sequence 7 +strand 545 n (File: SGN-E251179+) 1 TCAATTCAAT TTTCTAAATA TAAAAAAATA GATCACTTTA GAGTTGCAAT TGAAGGTATG 61 ATTGAAAAAT TTAAAAAATA TTTTTTTCCT ATTCCTCAAG TTTTTTTAAC GGCTACTTTA 121 CTTCATCCTG CTTATAAATT ACAAGGTGTG AAAGGCCTTG TCGACACTTT TTATGAAACT 181 TTAGAAGTTT TACCGGAAGA AACTCCAGAT TGTGAAACGT GTAAGTATAG CATAAAAATA 241 GAAGCAAAAA TTTTGTATGA AAAATATAGA ACTAGTGAAA ATTTTGAAGG AGAAGTTGCT 301 CAAACTTCTA ATGTTGAGAT TGATTTGTCA GTTCCAATTT CATGTTATAT GCGAGGTTTT 361 CTTGGTCTTA ATTCTACTAA CCGTGATGAT TTTGAAGAAT ATCTTAATCA ATCTTTAGAA 421 AACTTGGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT GGTGGAGTAG ACGAAGCGAT 481 GCATTTCCAA CTTTGAGCAA AATGGTTCGT GATATTCTAG CTATTCAAGC TTCATCAGTT 541 GCATC Predicted gene structure (within gDNA segment 14804 to 13032): Exon 1 14194 13652 ( 543 n); cDNA 2 544 ( 543 n); score: 0.898 MATCH C06HBa0046G10.1-1- SGN-E251179+ 0.898 543 0.996 C PGS_C06HBa0046G10.1-1-_SGN-E251179+ (14194 13652) Alignment (genomic DNA sequence = upper lines): CAATTCAATT TTCTAAATAT AAAAAAATAG ATAAATTTAA AGTTGCAATT GAAGGTATGA 14135 |||||||||| |||||||||| |||||||||| || | |||| |||||||||| |||||||||| CAATTCAATT TTCTAAATAT AAAAAAATAG ATCACTTTAG AGTTGCAATT GAAGGTATGA 61 TTGAAAAATT AAAAAAATAT TTTTTTCTTA TTCCTCAAAT TTATTTAACT GCTACTTTAC 14075 |||||||||| ||||||||| ||||||| || |||||||| | || |||||| |||||||||| TTGAAAAATT TAAAAAATAT TTTTTTCCTA TTCCTCAAGT TTTTTTAACG GCTACTTTAC 121 TTTCATCCTG ATTATAAATT ACGAGGTGTG CAGGGCCTTA TTGACACTTT TTGTGAAACT 14015 ||||||||| ||||||||| || ||||||| | |||||| | |||||||| || ||||||| -TTCATCCTG CTTATAAATT ACAAGGTGTG AAAGGCCTTG TCGACACTTT TTATGAAACT 180 TAAGAAATTT TACCTAAAGA AATTCCAAAT TGTGAAACGT GTAAGTCTAG CATAAAAGTA 13955 | |||| ||| |||| |||| || |||| || |||||||||| |||||| ||| ||||||| || TTAGAAGTTT TACCGGAAGA AACTCCAGAT TGTGAAACGT GTAAGTATAG CATAAAAATA 240 GAAGCAAAAA TTTTGTATAA AAAATATAGA ACTAGTGAAA ATATTGAAGG AGAAGTTGGT 13895 |||||||||| |||||||| | |||||||||| |||||||||| || ||||||| |||||||| | GAAGCAAAAA TTTTGTATGA AAAATATAGA ACTAGTGAAA ATTTTGAAGG AGAAGTTGCT 300 CAAACTTCTA ATCAAGATAT TGATTTTGAA GTTCGAATTT CAACTTATAT GAGAGGTTTT 13835 |||||||||| || || || |||||| | |||| ||||| || |||||| | |||||||| CAAACTTCTA ATGTTGAGAT TGATTTGTCA GTTCCAATTT CATGTTATAT GCGAGGTTTT 360 CTTTGTCTTA ATTCTACTAA CCGTGATGAT TTAGAAGAAT ATATTAATCA ATCTTTAGAA 13775 ||| |||||| |||||||||| |||||||||| || ||||||| || ||||||| |||||||||| CTTGGTCTTA ATTCTACTAA CCGTGATGAT TTTGAAGAAT ATCTTAATCA ATCTTTAGAA 420 GTATTTGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT -GTGTAGTAG GCGAACCGTT 13716 || |||| |||||||||| |||||||||| |||||||||| ||| ||||| |||| || | AACTTGGAAA TCAAAGATGG CAATGAAGAT TTATTAGGAT GGTGGAGTAG ACGAAGCGAT 480 GCATTTCCAA CTTTGAGCAA AATGATTTGT GATGTTCTAG TTATTCAAGC TTCATCAGTT 13656 |||||||||| |||||||||| |||| || || ||| |||||| ||||||||| |||||||||| GCATTTCCAA CTTTGAGCAA AATGGTTCGT GATATTCTAG CTATTCAAGC TTCATCAGTT 540 GCAT 13652 |||| GCAT 544 hqPGS_C06HBa0046G10.1-1-_SGN-E251179+ (14194 13652) ******************************************************************************** EST sequence 16 +strand 561 n (File: SGN-E238157+) 1 TGGAATTCTC TTTACGAAAT GCTTTCGGTT GCTTATAAAT ATAGAAGACC CATACAAATG 61 GTTTTTAATG CCATAATGCT GACCCATTAG ATAGAATTTG TGATGAAGAT TGGGAAGAAA 121 CTAAAGAACG ATTACAATTT TTAAGACTTT TTTATGATGC TACTACCATG TTTTCGGGAA 181 TTTATTATCC TACTATTTCA TCCGTATTAA TAAATATTTG TGCTCTTTCA ATTCGATTTT 241 GTAAATATAA AAAAATAGAT AAATTTAGAG TTGCAATTGA AGGTATGATT GAAACATTTT 301 AAAAATATTT TTTTCCTATT CCTCAAGCTT TTTTAACGGC TACTTTACTT CATCCTGCTT 361 ATAAATTACA AGGTGTGCAA GGCCTTGTTG ACACTTTTTA TAAAAAGTTA GAAATTTTAC 421 CGGAAGAAAT TTTAAATTGT CAAGCGTGTA GTCTAGCATA AAAGTAGAAG CAAAAGTTTT 481 GTATGAAAAA TATAGAACTA TTGAAAATTT TCAAGGAGAA GTTGGTCAAA CATCTAATGT 541 TGAGATTGAT TCGTCACTTC C Predicted gene structure (within gDNA segment 15346 to 10849): Exon 1 14422 13861 ( 562 n); cDNA 1 560 ( 560 n); score: 0.889 MATCH C06HBa0046G10.1-1- SGN-E238157+ 0.889 562 1.002 C PGS_C06HBa0046G10.1-1-_SGN-E238157+ (14422 13861) Alignment (genomic DNA sequence = upper lines): TGGAATTCAT TTTACGAAAT GTTTTCAGTT GCTTACAAAT ATAGAAGACC CATACAAAT- 14364 |||||||| |||||||||| | |||| ||| ||||| |||| |||||||||| ||||||||| TGGAATTCTC TTTACGAAAT GCTTTCGGTT GCTTATAAAT ATAGAAGACC CATACAAATG 60 GTGTTTAATG TTCATAATGC TGACCCATTA GATAGAATTT GTGATGAAGA TTGGGAAGAA 14304 || ||||||| |||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTTAATG -CCATAATGC TGACCCATTA GATAGAATTT GTGATGAAGA TTGGGAAGAA 119 ACTAAAGACC TATTACAATT TTTAAGACTT TTTTATGATG CAACAATCAT GTTTTCTGGA 14244 |||||||| | ||||||||| |||||||||| |||||||||| | || | ||| |||||| ||| ACTAAAGAAC GATTACAATT TTTAAGACTT TTTTATGATG CTACTACCAT GTTTTCGGGA 179 ATTTATTATC CTACTATTTT ATCAGTATTA ATAAATATTT GTGCTCTTAC AATTCAATTT 14184 |||||||||| ||||||||| ||| |||||| |||||||||| |||||||| | ||||| |||| ATTTATTATC CTACTATTTC ATCCGTATTA ATAAATATTT GTGCTCTTTC AATTCGATTT 239 TCTAAATATA AAAAAATAGA TAAATTTAAA GTTGCAATTG AAGGTATGAT TGAAAAATTA 14124 | |||||||| |||||||||| |||||||| | |||||||||| |||||||||| ||||| ||| TGTAAATATA AAAAAATAGA TAAATTTAGA GTTGCAATTG AAGGTATGAT TGAAACATTT 299 AAAAAATATT TTTTTCTTAT TCCTCAAATT TATTTAACTG CTACTTTACT TTCATCCTGA 14064 ||||||||| |||||| ||| ||||||| | | |||||| | ||||||||| ||||||||| TAAAAATATT TTTTTCCTAT TCCTCAAGCT TTTTTAACGG CTACTTTAC- TTCATCCTGC 358 TTATAAATTA CGAGGTGTGC AGGGCCTTAT TGACACTTTT TGTGAAACTT AAGAAATTTT 14004 |||||||||| | |||||||| | |||||| | |||||||||| | | ||| | ||||||||| TTATAAATTA CAAGGTGTGC AAGGCCTTGT TGACACTTTT TATAAAAAGT TAGAAATTTT 418 ACCTAAAGAA ATTCCAAATT GTGAAACGTG TAAGTCTAGC ATAAAAGTAG AAGCAAAAAT 13944 ||| ||||| ||| ||||| || || |||| | |||||||| |||||||||| |||||||| | ACCGGAAGAA ATTTTAAATT GTCAAGCGTG T-AGTCTAGC ATAAAAGTAG AAGCAAAAGT 477 TTTGTATAAA AAATATAGAA CTAGTGAAAA TATTGAAGGA GAAGTTGGTC AAACTTCTAA 13884 ||||||| || |||||||||| ||| |||||| | || ||||| |||||||||| |||| ||||| TTTGTATGAA AAATATAGAA CTATTGAAAA TTTTCAAGGA GAAGTTGGTC AAACATCTAA 537 TCAAGATATT GATTTTGAAG TTC 13861 | || ||| |||| | ||| TGTTGAGATT GATTCGTCAC TTC 560 hqPGS_C06HBa0046G10.1-1-_SGN-E238157+ (14422 13861) ******************************************************************************** EST sequence 12 +strand 444 n (File: SGN-E279072+) 1 CAAATGTTTT TTAATGCTTA TAATGCTGAC CAGTTAGATA GAATTTGTGA TGAAGACTGG 61 GAAGAAACTA AAGAACTATT ACAGTTTTTA AGACTTTTTT ATGATGCTAC TACCATGTTT 121 TCTAGAATTT ATTATCCTAC TATTTCATCC GTATTAATAA ATATTTGTGC TCTTACAATT 181 CAATTTTGTA AATATAAAAA AATAGATAAA TTTAGAGTTG CAATTGAAGG TATGGTTGAA 241 AAATTTTAAA AATATTTTTT TACTATTCGT CAAGTTTTTT TTACCGGCTA CTTTACTTCA 301 TCCCGCTTAT AAATTACAAG GTTTGCAAGA CCTTGTTGAC ACATTTTATG AAACTTAAGA 361 AATTTTACCG GAAGAAATTT CAAATTGTCA AACGTGTTGA GTCTAGCATA ACAGTAGAAG 421 CAAAGCTGAA AAATATAAAA CTAC Predicted gene structure (within gDNA segment 15266 to 11426): Exon 1 14368 13931 ( 438 n); cDNA 1 440 ( 440 n); score: 0.869 MATCH C06HBa0046G10.1-1- SGN-E279072+ 0.869 438 0.986 C PGS_C06HBa0046G10.1-1-_SGN-E279072+ (14368 13931) Alignment (genomic DNA sequence = upper lines): CAAATG-TGT TTAATGTTCA TAATGCTGAC CCATTAGATA GAATTTGTGA TGAAGATTGG 14310 |||||| | | |||||| | | |||||||||| | ||||||| |||||||||| |||||| ||| CAAATGTTTT TTAATGCTTA TAATGCTGAC CAGTTAGATA GAATTTGTGA TGAAGACTGG 60 GAAGAAACTA AAGACCTATT ACAATTTTTA AGACTTTTTT ATGATGCAAC AATCATGTTT 14250 |||||||||| |||| ||||| ||| |||||| |||||||||| ||||||| || | ||||||| GAAGAAACTA AAGAACTATT ACAGTTTTTA AGACTTTTTT ATGATGCTAC TACCATGTTT 120 TCTGGAATTT ATTATCCTAC TATTTTATCA GTATTAATAA ATATTTGTGC TCTTACAATT 14190 ||| |||||| |||||||||| ||||| ||| |||||||||| |||||||||| |||||||||| TCTAGAATTT ATTATCCTAC TATTTCATCC GTATTAATAA ATATTTGTGC TCTTACAATT 180 CAATTTTCTA AATATAAAAA AATAGATAAA TTTAAAGTTG CAATTGAAGG TATGATTGAA 14130 ||||||| || |||||||||| |||||||||| |||| ||||| |||||||||| |||| ||||| CAATTTTGTA AATATAAAAA AATAGATAAA TTTAGAGTTG CAATTGAAGG TATGGTTGAA 240 AAATTAAAAA AATATTTTTT TCTTATTCCT CAA-ATTTAT TTAACTGCTA CTTTACTTTC 14071 ||||| ||| |||||||||| | ||||| | ||| ||| | ||| | |||| |||||| ||| AAATTTTAAA AATATTTTTT TACTATTCGT CAAGTTTTTT TTACCGGCTA CTTTAC-TTC 299 ATCCTGATTA TAAATTACGA GGTGTGCAGG GCCTTATTGA CACTTTTTGT GAAACTTAAG 14011 |||| | ||| |||||||| | ||| |||| | |||| |||| ||| |||| | |||||||||| ATCCCGCTTA TAAATTACAA GGTTTGCAAG ACCTTGTTGA CACATTTTAT GAAACTTAAG 359 AAATTTTACC TAAAGAAATT CCAAATTGTG AAACGTG-TA AGTCTAGCAT AAAAGTAGAA 13952 |||||||||| |||||||| |||||||| ||||||| | |||||||||| || ||||||| AAATTTTACC GGAAGAAATT TCAAATTGTC AAACGTGTTG AGTCTAGCAT AACAGTAGAA 419 GCAAAAATTT TGTATAAAAA A 13931 ||||| | ||| ||| | GCAAAGCTGA AAAATATAAA A 440 hqPGS_C06HBa0046G10.1-1-_SGN-E279072+ (14368 13931) ******************************************************************************** EST sequence 5 +strand 745 n (File: SGN-E349085+) 1 AAATGCTTAT TTGATCCCCC CGTTCCACCT TGACCCCCCC ACCTTGTTTA TGTTTAAAAG 61 GTTGCTTACA CTTATTACAG ATAGCAACAC TATTTTCTTT ATCAAAAGTC ATAAATTGCC 121 ACACAATAGA AGTTTTAAGT CGTTGATACT TCACCTTATC CCTTGTGGGA GGTATAAGGG 181 GTGGATGCTC AAAATTTTGC TCCGGTAAAT TGATAGTGTC ATTTGAAACT TCTGTCACCG 241 GACTAGTAGG TGTTTCATAT AACTCTTCTT CTCCGAAATG AGTTTCTACT TCTTCCCCTG 301 AATTTTCATC AACAATAGGA TTAATATTAC CATAACGTCG TTCTAAACTT TCGTGATCGA 361 GTTGAACATT TGAATCAATA TTATAAGGAG TATTATCCAC ATAAGTAGAA TCATTTATGT 421 TAAATCTAAC TTTACTCGTT GATTTAGATG AAGTACCACC ACTTCTACTA CTCTTTTTTT 481 CTTTACTATT TTTTTTTATC AAAATTAAAT AACTTAAAAA GTCTACTTTC TTCGTTATCT 541 TGTGCTTTCT CTTTTCCTTA ACCACTATTT TTTTGCTAGA ACTCATACTT AATTTTATAT 601 ATATATATAT ATATATATAT ATATATATAT ATATATATAT ATAAAGGGTA ATATAAAAAT 661 AATAATAACA CAANAGTAAA GACGAATATT TGAAACGAAT GTACCAAACA TCTGCGTTAA 721 TAGCAGACGC TAAACCGATT GTTGA Predicted gene structure (within gDNA segment 14386 to 16772): Exon 1 15304 15918 ( 615 n); cDNA 1 617 ( 617 n); score: 0.861 Intron 1 15919 16083 ( 165 n); Pd: 0.000 (s: 0.86), Pa: 0.000 (s: 0.86) Exon 2 16084 16126 ( 43 n); cDNA 618 660 ( 43 n); score: 0.860 MATCH C06HBa0046G10.1-1+ SGN-E349085+ 0.861 658 0.883 C PGS_C06HBa0046G10.1-1+_SGN-E349085+ (15304 15918,16084 16126) Alignment (genomic DNA sequence = upper lines): AAATGTCTAT TTAAT-CCCC CGTCCCACCT TGA-CC-CCC ACCTTGTTTA TGTTTAAAAG 15360 ||||| ||| || || |||| ||| |||||| ||| || ||| |||||||||| |||||||||| AAATGCTTAT TTGATCCCCC CGTTCCACCT TGACCCCCCC ACCTTGTTTA TGTTTAAAAG 60 GTTGCTTACA CTTATTACAA AATAGCAACA CTATTTTCTT TATCGAAAGT CATAAATTGC 15420 |||||||||| |||||||| | ||||||||| |||||||||| |||| ||||| |||||||||| GTTGCTTACA CTTATTAC-A GATAGCAACA CTATTTTCTT TATCAAAAGT CATAAATTGC 119 CACACAATAT AAGTTTTAGG GCGTTGATAC TTCACCTTCT CCCTTGTGAG AGGTATAAGG 15480 ||||||||| |||||||| | ||||||||| |||||||| | |||||||| | |||||||||| CACACAATAG AAGTTTTAAG TCGTTGATAC TTCACCTTAT CCCTTGTGGG AGGTATAAGG 179 AGTGGACGTC CACAATTTTG CTCCGTCGAA TTTATAGTGT CATTTGGAAC TTCTGTCACC 15540 ||||| | || ||||||| ||||| || || ||||||| |||||| ||| |||||||||| GGTGGATGCT CAAAATTTTG CTCCGGTAAA TTGATAGTGT CATTTGAAAC TTCTGTCACC 239 GGACTAGTAG GTGTATCTTC TAAATCCTTT TCTCACAAAT CAGCTTCTAC TTCTTCACCT 15600 |||||||||| |||| || | ||| || | | |||| |||| || |||||| |||||| ||| GGACTAGTAG GTGTTTCATA TAACTCTTCT TCTCCGAAAT GAGTTTCTAC TTCTTCCCCT 299 GAATTTTCAT CAACATTAGG ATTAATATTA CCATAACTTC GTTCTAAACT TTGATGATCG 15660 |||||||||| ||||| |||| |||||||||| ||||||| || |||||||||| || |||||| GAATTTTCAT CAACAATAGG ATTAATATTA CCATAACGTC GTTCTAAACT TTCGTGATCG 359 AGTTGAACAT TTGAATTAAT ATCATAAGGA G---TATCAA CATAAGTAGA ATCATTTGGG 15717 |||||||||| |||||| ||| || ||||||| | |||| | |||||||||| ||||||| | AGTTGAACAT TTGAATCAAT ATTATAAGGA GTATTATCCA CATAAGTAGA ATCATTTATG 419 TTAAATCTAA CTCTACACGT TGATTTATAT GAAGTACCAC CACTTTTACT ACTCTTTTTT 15777 |||||||||| || ||| ||| ||||||| || |||||||||| ||||| |||| |||||||||| TTAAATCTAA CTTTACTCGT TGATTTAGAT GAAGTACCAC CACTTCTACT ACTCTTTTTT 479 TCTTTATTA- TTTTTTTTAC TAAAATTAAA TATTTTAAAA ATTCCACTTT GTTCGTTATC 15836 |||||| || ||||||||| ||||||||| || |||||| | || ||||| ||||||||| TCTTTACTAT TTTTTTTTAT CAAAATTAAA TAACTTAAAA AGTCTACTTT CTTCGTTATC 539 TTGTTCTTTT TCTTTTCCTT TACCGATATT TTTTTTTGCT AGAACTCATA CTTAATTATA 15896 |||| |||| |||||||||| ||| | | |||||||||| |||||||||| ||||||| || TTGTGCTTTC TCTTTTCCTT AACC-A-CTA TTTTTTTGCT AGAACTCATA CTTAATTTTA 597 TATAAAATGT AATATAAAAA TAATAATAAC ACAAAAAATA AAGACGAAAA ATAAAATATA 15956 ||| | || | ||||| | | || TAT-ATATAT -ATATATATA TA........ .......... .......... .......... 617 AATATTATGA AACGAATGTA CCAAACGTCT GCGTTAATAG CAGACGTTAA ATCGATTCTT 16016 .......... .......... .......... .......... .......... .......... 617 GAAGCTTATG ATTTGTTGTA ACTTGTAATT TGAAACTCCA ACCAATACTT GATAACAAAT 16076 .......... .......... .......... .......... .......... .......... 617 ATGAGATTTT ATATATATAT ATATATATAT ATATAAAATA ATATGAAAAT 16126 | | |||||||||| |||||||||| ||| | || |||| ||||| .......TAT ATATATATAT ATATATATAT ATAAAGGGTA ATATAAAAAT 660 hqPGS_C06HBa0046G10.1-1+_SGN-E349085+ (15304 15918,16084 16126) ******************************************************************************** EST sequence 1 +strand 738 n (File: SGN-E350209+) 1 AAATGCTTAT TTGATCCCCC CGTTCCACCT TGACCCCCCC ACCTTGTTTA TGTTTAAAAG 61 GTTGCTTACA CTTATTACAG ATAGCAACAC TATTTTCTTT ATCAAAAGTC ATAAATTGCC 121 ACACAATAGA AGTTTTAAGT CGTTGATACT TCACCTTATC CCTTGTGGGA GGTATAAGGG 181 GTGGATGCTC AAAATTTTGC TCCGGTAAAT TGATAGTGTC ATTTGAAACT TCTGTCACCG 241 GACTAGTAGG TGTTTCATAT AACTCTTCTT CTCCGAAATG AGTTTCTACT TCTTCCCCTG 301 AATTTTCATC AACAATAGGA TTAATATTAC CATAACGTCG TTCTAAACTT TCGTGATCGA 361 GTTGAACATT TGAATCAATA TTATAAGGAG TATTATCCAC ATAAGTAGAA TCATTTATGT 421 TAAATCTAAC TTTACTCGTT GATTTAGATG AAGTACCACC ACTTCTACTA CTCTTTTTTT 481 CTTTACTATT TTTTTTTATC AAAATTAAAT AACTTAAAAA GTCTACTTTC TTCGTTATCT 541 TGTGCTTTCT CTTTTCCTTA ACCACTATTT TTTTGCTAGA ACTCATACTT AATTTTATAT 601 ATATATATAT ATATATATAT ATATATATAT ATATATATAT ANTAAGTGGT ATATAAAAAT 661 AATAATAACA CAAAAGTNAA GACGAATATT TGAAACGAAT GTACCAAACA TCTGCGTTAA 721 TAGCAGACGC TAAACCGA Predicted gene structure (within gDNA segment 14386 to 17302): Exon 1 15304 15918 ( 615 n); cDNA 1 617 ( 617 n); score: 0.861 Intron 1 15919 16085 ( 167 n); Pd: 0.000 (s: 0.86), Pa: 0.000 (s: 0) Exon 2 16086 16124 ( 39 n); cDNA 618 656 ( 39 n); score: 0.821 MATCH C06HBa0046G10.1-1+ SGN-E350209+ 0.861 654 0.886 C PGS_C06HBa0046G10.1-1+_SGN-E350209+ (15304 15918,16086 16124) Alignment (genomic DNA sequence = upper lines): AAATGTCTAT TTAAT-CCCC CGTCCCACCT TGA-CC-CCC ACCTTGTTTA TGTTTAAAAG 15360 ||||| ||| || || |||| ||| |||||| ||| || ||| |||||||||| |||||||||| AAATGCTTAT TTGATCCCCC CGTTCCACCT TGACCCCCCC ACCTTGTTTA TGTTTAAAAG 60 GTTGCTTACA CTTATTACAA AATAGCAACA CTATTTTCTT TATCGAAAGT CATAAATTGC 15420 |||||||||| |||||||| | ||||||||| |||||||||| |||| ||||| |||||||||| GTTGCTTACA CTTATTAC-A GATAGCAACA CTATTTTCTT TATCAAAAGT CATAAATTGC 119 CACACAATAT AAGTTTTAGG GCGTTGATAC TTCACCTTCT CCCTTGTGAG AGGTATAAGG 15480 ||||||||| |||||||| | ||||||||| |||||||| | |||||||| | |||||||||| CACACAATAG AAGTTTTAAG TCGTTGATAC TTCACCTTAT CCCTTGTGGG AGGTATAAGG 179 AGTGGACGTC CACAATTTTG CTCCGTCGAA TTTATAGTGT CATTTGGAAC TTCTGTCACC 15540 ||||| | || ||||||| ||||| || || ||||||| |||||| ||| |||||||||| GGTGGATGCT CAAAATTTTG CTCCGGTAAA TTGATAGTGT CATTTGAAAC TTCTGTCACC 239 GGACTAGTAG GTGTATCTTC TAAATCCTTT TCTCACAAAT CAGCTTCTAC TTCTTCACCT 15600 |||||||||| |||| || | ||| || | | |||| |||| || |||||| |||||| ||| GGACTAGTAG GTGTTTCATA TAACTCTTCT TCTCCGAAAT GAGTTTCTAC TTCTTCCCCT 299 GAATTTTCAT CAACATTAGG ATTAATATTA CCATAACTTC GTTCTAAACT TTGATGATCG 15660 |||||||||| ||||| |||| |||||||||| ||||||| || |||||||||| || |||||| GAATTTTCAT CAACAATAGG ATTAATATTA CCATAACGTC GTTCTAAACT TTCGTGATCG 359 AGTTGAACAT TTGAATTAAT ATCATAAGGA G---TATCAA CATAAGTAGA ATCATTTGGG 15717 |||||||||| |||||| ||| || ||||||| | |||| | |||||||||| ||||||| | AGTTGAACAT TTGAATCAAT ATTATAAGGA GTATTATCCA CATAAGTAGA ATCATTTATG 419 TTAAATCTAA CTCTACACGT TGATTTATAT GAAGTACCAC CACTTTTACT ACTCTTTTTT 15777 |||||||||| || ||| ||| ||||||| || |||||||||| ||||| |||| |||||||||| TTAAATCTAA CTTTACTCGT TGATTTAGAT GAAGTACCAC CACTTCTACT ACTCTTTTTT 479 TCTTTATTA- TTTTTTTTAC TAAAATTAAA TATTTTAAAA ATTCCACTTT GTTCGTTATC 15836 |||||| || ||||||||| ||||||||| || |||||| | || ||||| ||||||||| TCTTTACTAT TTTTTTTTAT CAAAATTAAA TAACTTAAAA AGTCTACTTT CTTCGTTATC 539 TTGTTCTTTT TCTTTTCCTT TACCGATATT TTTTTTTGCT AGAACTCATA CTTAATTATA 15896 |||| |||| |||||||||| ||| | | |||||||||| |||||||||| ||||||| || TTGTGCTTTC TCTTTTCCTT AACC-A-CTA TTTTTTTGCT AGAACTCATA CTTAATTTTA 597 TATAAAATGT AATATAAAAA TAATAATAAC ACAAAAAATA AAGACGAAAA ATAAAATATA 15956 ||| | || | ||||| | | || TAT-ATATAT -ATATATATA TA........ .......... .......... .......... 617 AATATTATGA AACGAATGTA CCAAACGTCT GCGTTAATAG CAGACGTTAA ATCGATTCTT 16016 .......... .......... .......... .......... .......... .......... 617 GAAGCTTATG ATTTGTTGTA ACTTGTAATT TGAAACTCCA ACCAATACTT GATAACAAAT 16076 .......... .......... .......... .......... .......... .......... 617 ATGAGATTTT ATATATATAT ATATATATAT ATATAAAATA ATATGAAA 16124 | |||||||||| |||||||||| ||| || | ||| || .........T ATATATATAT ATATATATAT ATANTAAGTG GTATATAA 656 hqPGS_C06HBa0046G10.1-1+_SGN-E350209+ (15304 15918,16086 16124) ******************************************************************************** EST sequence 9 -strand 777 n (File: SGN-E378787-) 1 GCAGCATTTG TATTATTAGA TGCATTATCT AATGCGACAG ACATTATTTT ATCTATAATC 61 ATGTAATAAT CTAAAACACT TAAAATATTT GAAGCTAAAT ATGCACCAGT TTTTTTCTCT 121 ATACATAGTT TATAACATAT AATTCTTTTT TGCAAATTCC AATTATGATC AATCCAATGT 181 GCAGTTACTG TTAAATGATC ATTTCCATTA ACACTACGAC CCATGTCCGA AGTAATAGAC 241 ACTCTACTAT CTACTATGCT AAATAGACAA CGAAGAAATT GACAATGTTT ACCTTGATGT 301 TCAAAAACAT CAGTTTTGAC AGTATTTCTT GAAAAGCCTC TATAATCCGG ATTATAAGTT 361 TGTTGAATAT ATTCGACAAA ACCGGGATTT GAAGGAAAAC TATAAGGCAA ACCACAAACA 421 GAAACCATTT TAGCTAAATT TTCCCGATCT AATTCCTTAT TATATTTACG TTGTATTATT 481 GGACCAGCGG GGTTAAAAGG ATCTAATGTT CCTTGAACCG TTAGAAGCCG CTCTCGATGC 541 ATTACCGGTA ATATCTAATT CATTAATTGA AATTCCCTTT TTTCTATTGG CTAATGCTTT 601 AGCTTCTTTA TATTGAATCG GCACACAAGA TAACAAATGT CTATTTAATC CCCCCGTTCC 661 ACCTTGACCC CCACCTTGTT TATGTTTAAA AGCTTGCTTA CACTTATTAC AAATAGCAAC 721 ACTATTTTCT TTATCGTAAG TCATAAATTG CCACACAATA GAAGTTTTAG GTCGTTG Predicted gene structure (within gDNA segment 13529 to 16199): Exon 1 14672 15446 ( 775 n); cDNA 3 777 ( 775 n); score: 0.905 MATCH C06HBa0046G10.1-1+ SGN-E378787- 0.905 775 0.997 C PGS_C06HBa0046G10.1-1+_SGN-E378787- (14672 15446) Alignment (genomic DNA sequence = upper lines): AGCGTTTGTA TTGTTAGATG CATTATCTAA TGCAACAGAC ATTACTTTAT CTGTAACCAT 14731 ||| |||||| || ||||||| |||||||||| ||| |||||| |||| ||||| || ||| ||| AGCATTTGTA TTATTAGATG CATTATCTAA TGCGACAGAC ATTATTTTAT CTATAATCAT 62 GTAATAATCT AAAACACTTA AAATTTTTGA AGCTAAGTAT GCACCAG-TT TTTTCTCTAC 14790 |||||||||| |||||||||| |||| ||||| |||||| ||| ||||||| || ||||||||| GTAATAATCT AAAACACTTA AAATATTTGA AGCTAAATAT GCACCAGTTT TTTTCTCTAT 122 ACATAGTGTA TAACTTAATA TTCTTTTTTG CAAATTCCAA TTATGATCAA TCCAATGAGT 14850 ||||||| || |||| || | |||||||||| |||||||||| |||||||||| ||||||| | ACATAGTTTA TAACATATAA TTCTTTTTTG CAAATTCCAA TTATGATCAA TCCAATGTGC 182 TGAAACAGTT AAATAATCAT TTCCATTGAC ACTACGACCC ATATCCGTAG TTATAGACAC 14910 | || ||| |||| ||||| ||||||| || |||||||||| || |||| || | |||||||| AGTTACTGTT AAATGATCAT TTCCATTAAC ACTACGACCC ATGTCCGAAG TAATAGACAC 242 TCTACTATAT AATATGCTAA ATATACAACG AAGAAATTAA CAATGTTTAC CTTGAAATTC 14970 |||||||| | | |||||||| ||| |||||| |||||||| | |||||||||| ||||| ||| TCTACTATCT ACTATGCTAA ATAGACAACG AAGAAATTGA CAATGTTTAC CTTGATGTTC 302 AAAAACATCA TGTTTAACAG TATTTCTTGA AAA-CCTCTA TAATTCGGAT TATAAGTTTG 15029 |||||||||| ||| |||| |||||||||| ||| |||||| |||| ||||| |||||||||| AAAAACATCA GTTTTGACAG TATTTCTTGA AAAGCCTCTA TAATCCGGAT TATAAGTTTG 362 TTAAATTTAT TCAATAAAAT CGGGATATGA AGTAAAACTA TAAGGCAAAC TACAAACAGA 15089 || ||| ||| || | |||| |||||| ||| || ||||||| |||||||||| ||||||||| TTGAATATAT TCGACAAAAC CGGGATTTGA AGGAAAACTA TAAGGCAAAC CACAAACAGA 422 AACCATTTTA GCTAAATTTT CGCGATCAAA TTCCTTATTA TATTTACGTT GTGTTATTGG 15149 |||||||||| |||||||||| | ||||| || |||||||||| |||||||||| || ||||||| AACCATTTTA GCTAAATTTT CCCGATCTAA TTCCTTATTA TATTTACGTT GTATTATTGG 482 ACCACCGGGG CTAGAAAGAT CTAATGTTCC TTGAACTATG TGAGAAGCCC CTACCGATGC 15209 |||| ||||| || || ||| |||||||||| |||||| | | ||||||| || |||||| ACCAGCGGGG TTAAAAGGAT CTAATGTTCC TTGAAC--CG TTAGAAGCCG CTCTCGATGC 540 ACTACCGGTA ATATCAAATT CATCAATTGA AATTCCTTTT TTTCTATTGG CTAATGCTTT 15269 | |||||||| ||||| |||| ||| |||||| |||||| ||| |||||||||| |||||||||| ATTACCGGTA ATATCTAATT CATTAATTGA AATTCCCTTT TTTCTATTGG CTAATGCTTT 600 AGCTTCTTTA TATTGAATCG GCACACAAGA TAACAAATGT CTATTTAAT- CCCCCGTCCC 15328 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ||||||| || AGCTTCTTTA TATTGAATCG GCACACAAGA TAACAAATGT CTATTTAATC CCCCCGTTCC 660 ACCTTGACCC CCACCTTGTT TATGTTTAAA AGGTTGCTTA CACTTATTAC AAAATAGCAA 15388 |||||||||| |||||||||| |||||||||| || ||||||| |||||||||| ||||||||| ACCTTGACCC CCACCTTGTT TATGTTTAAA AGCTTGCTTA CACTTATTAC -AAATAGCAA 719 CACTATTTTC TTTATCGAAA GTCATAAATT GCCACACAAT ATAAGTTTTA GGGCGTTG 15446 |||||||||| ||||||| || |||||||||| |||||||||| | |||||||| || ||||| CACTATTTTC TTTATCGTAA GTCATAAATT GCCACACAAT AGAAGTTTTA GGTCGTTG 777 hqPGS_C06HBa0046G10.1-1+_SGN-E378787- (14672 15446) ******************************************************************************** EST sequence 11 -strand 640 n (File: SGN-E392586-) 1 TATGATCAAT CCAATGTGCA GTTACTGTTA AATGATCATT TCCATTAACA CTACGACCCA 61 TGTCCGAAGT AATAGACACT CTACTATTTA CTATGCTAAA TAGACAACGA AGAAATTGAC 121 AATGTTTACC TTGATGTTCA AAAACATCAG TTTTGACAGT ATTTCTTGAA AAGCCTCTAT 181 AATCCGGATT ATAAGTTTGT TGAATATATT CGACAAAACC GGGATTTGAA GGAAAACTAT 241 AAGGCAAACC ACAAACAGAA ACCATTTTAG CTAAATTTTC CCGATCTAAT TCCTTATTAT 301 ATTTACGTTG TATTATTGGA CCAGCGGGGT TAAAAGGATC TAATGTTCCT TGAACCGTTA 361 GAAGCCGCTC TCGATGCATT ACCGGTAATA TCTAATTCAT TAATTGAAAT TCCCTTTTTT 421 CTATTGGCTA ATGCTTTAGC TTCTTTATAT TGAATCGGCA CACAAGATAA CAAATGTCTA 481 TTTAATCCCC CCGTTCCACC TTGACCCCCA CCTTGTTTAT GTTTAAAAGC TTGCTTACAC 541 TTATTACAAA TAGCAACACT ATTTTCTTTA TCGTAAGTCA TAAATTGCCA CACAATAGAA 601 GTTTTAGGTC GTTGATACTT CACCTTCTCC CTTGTGGGAG Predicted gene structure (within gDNA segment 12827 to 16108): Exon 1 14832 15472 ( 641 n); cDNA 1 640 ( 640 n); score: 0.908 MATCH C06HBa0046G10.1-1+ SGN-E392586- 0.908 641 1.002 C PGS_C06HBa0046G10.1-1+_SGN-E392586- (14832 15472) Alignment (genomic DNA sequence = upper lines): TATGATCAAT CCAATGAGTT GAAACAGTTA AATAATCATT TCCATTGACA CTACGACCCA 14891 |||||||||| |||||| | | || |||| ||| |||||| |||||| ||| |||||||||| TATGATCAAT CCAATGTGCA GTTACTGTTA AATGATCATT TCCATTAACA CTACGACCCA 60 TATCCGTAGT TATAGACACT CTACTATATA ATATGCTAAA TATACAACGA AGAAATTAAC 14951 | |||| ||| ||||||||| ||||||| || ||||||||| || ||||||| ||||||| || TGTCCGAAGT AATAGACACT CTACTATTTA CTATGCTAAA TAGACAACGA AGAAATTGAC 120 AATGTTTACC TTGAAATTCA AAAACATCAT GTTTAACAGT ATTTCTTGAA AA-CCTCTAT 15010 |||||||||| |||| |||| ||||||||| ||| ||||| |||||||||| || ||||||| AATGTTTACC TTGATGTTCA AAAACATCAG TTTTGACAGT ATTTCTTGAA AAGCCTCTAT 180 AATTCGGATT ATAAGTTTGT TAAATTTATT CAATAAAATC GGGATATGAA GTAAAACTAT 15070 ||| |||||| |||||||||| | ||| |||| | | |||| | ||||| |||| | |||||||| AATCCGGATT ATAAGTTTGT TGAATATATT CGACAAAACC GGGATTTGAA GGAAAACTAT 240 AAGGCAAACT ACAAACAGAA ACCATTTTAG CTAAATTTTC GCGATCAAAT TCCTTATTAT 15130 ||||||||| |||||||||| |||||||||| |||||||||| ||||| ||| |||||||||| AAGGCAAACC ACAAACAGAA ACCATTTTAG CTAAATTTTC CCGATCTAAT TCCTTATTAT 300 ATTTACGTTG TGTTATTGGA CCACCGGGGC TAGAAAGATC TAATGTTCCT TGAACTATGT 15190 |||||||||| | |||||||| ||| ||||| || || |||| |||||||||| ||||| || ATTTACGTTG TATTATTGGA CCAGCGGGGT TAAAAGGATC TAATGTTCCT TGAAC--CGT 358 GAGAAGCCCC TACCGATGCA CTACCGGTAA TATCAAATTC ATCAATTGAA ATTCCTTTTT 15250 ||||||| | | ||||||| ||||||||| |||| ||||| || ||||||| ||||| |||| TAGAAGCCGC TCTCGATGCA TTACCGGTAA TATCTAATTC ATTAATTGAA ATTCCCTTTT 418 TTCTATTGGC TAATGCTTTA GCTTCTTTAT ATTGAATCGG CACACAAGAT AACAAATGTC 15310 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCTATTGGC TAATGCTTTA GCTTCTTTAT ATTGAATCGG CACACAAGAT AACAAATGTC 478 TATTTAAT-C CCCCGTCCCA CCTTGACCCC CACCTTGTTT ATGTTTAAAA GGTTGCTTAC 15369 |||||||| | |||||| ||| |||||||||| |||||||||| |||||||||| | |||||||| TATTTAATCC CCCCGTTCCA CCTTGACCCC CACCTTGTTT ATGTTTAAAA GCTTGCTTAC 538 ACTTATTACA AAATAGCAAC ACTATTTTCT TTATCGAAAG TCATAAATTG CCACACAATA 15429 ||||||||| |||||||||| |||||||||| |||||| ||| |||||||||| |||||||||| ACTTATTAC- AAATAGCAAC ACTATTTTCT TTATCGTAAG TCATAAATTG CCACACAATA 597 TAAGTTTTAG GGCGTTGATA CTTCACCTTC TCCCTTGTGA GAG 15472 ||||||||| | |||||||| |||||||||| ||||||||| ||| GAAGTTTTAG GTCGTTGATA CTTCACCTTC TCCCTTGTGG GAG 640 hqPGS_C06HBa0046G10.1-1+_SGN-E392586- (14832 15472) ******************************************************************************** EST sequence 10 -strand 586 n (File: SGN-E392581-) 1 GACCCATGTC CGAAGTAATA GACACTTTAC TATCTATTAT GCTAAATAGA CAACGAAGAA 61 ATTGACAATG TTTACCTTGA TGTTCAAAAA CATCAGTTTT GACAGTATTT CTTGAAAAGC 121 CTCTATAATC CGGATTATAA GTTTGTTGAA TATATTCGAC AAAACCGGGA TTTGAAGGAA 181 AACTATAAGG CAAACCACAA ACAGAAACCA TTTTAGCTAA ATTTTCCCGA TCTAATTCCT 241 TATTATATTT ACGTTGTATT ATTGGACCAG CGGGGTTAAA AGGATCTAAT GTTCCTTGAA 301 CCGTTAGAAG CCGCTCTCGA TGCATTACCG GTAATATCTA ATTCATTAAT TGAAATTCCC 361 TTTTTTCTAT TGGCTAATGC TTTAGCTTCT TTATATTGAA TCGGCACACA AGATAACAAA 421 TGTCTATTTA ATCCCCCCGT TCCACCTTGA CCCCCACCTT GTTTATGTTT AAAAGCTTGC 481 TTACACTTAT TACAAATAGC AACACTATTT TCTTTATCGT AAGTCATAAA TTGCCACACA 541 ATAGAAGTTT TAGGTCGTTG ATACTTCACC TTCTCCCTTG TGGGAG Predicted gene structure (within gDNA segment 13367 to 16108): Exon 1 14886 15472 ( 587 n); cDNA 1 586 ( 586 n); score: 0.911 MATCH C06HBa0046G10.1-1+ SGN-E392581- 0.911 587 1.002 C PGS_C06HBa0046G10.1-1+_SGN-E392581- (14886 15472) Alignment (genomic DNA sequence = upper lines): GACCCATATC CGTAGTTATA GACACTCTAC TATATAATAT GCTAAATATA CAACGAAGAA 14945 ||||||| || || ||| ||| |||||| ||| ||| || ||| |||||||| | |||||||||| GACCCATGTC CGAAGTAATA GACACTTTAC TATCTATTAT GCTAAATAGA CAACGAAGAA 60 ATTAACAATG TTTACCTTGA AATTCAAAAA CATCATGTTT AACAGTATTT CTTGAAAA-C 15004 ||| |||||| |||||||||| |||||||| ||||| ||| ||||||||| |||||||| | ATTGACAATG TTTACCTTGA TGTTCAAAAA CATCAGTTTT GACAGTATTT CTTGAAAAGC 120 CTCTATAATT CGGATTATAA GTTTGTTAAA TTTATTCAAT AAAATCGGGA TATGAAGTAA 15064 ||||||||| |||||||||| ||||||| || | ||||| | |||| ||||| | ||||| || CTCTATAATC CGGATTATAA GTTTGTTGAA TATATTCGAC AAAACCGGGA TTTGAAGGAA 180 AACTATAAGG CAAACTACAA ACAGAAACCA TTTTAGCTAA ATTTTCGCGA TCAAATTCCT 15124 |||||||||| ||||| |||| |||||||||| |||||||||| |||||| ||| || ||||||| AACTATAAGG CAAACCACAA ACAGAAACCA TTTTAGCTAA ATTTTCCCGA TCTAATTCCT 240 TATTATATTT ACGTTGTGTT ATTGGACCAC CGGGGCTAGA AAGATCTAAT GTTCCTTGAA 15184 |||||||||| ||||||| || ||||||||| ||||| || | | |||||||| |||||||||| TATTATATTT ACGTTGTATT ATTGGACCAG CGGGGTTAAA AGGATCTAAT GTTCCTTGAA 300 CTATGTGAGA AGCCCCTACC GATGCACTAC CGGTAATATC AAATTCATCA ATTGAAATTC 15244 | || ||| |||| || | |||||| ||| |||||||||| ||||||| | |||||||||| C--CGTTAGA AGCCGCTCTC GATGCATTAC CGGTAATATC TAATTCATTA ATTGAAATTC 358 CTTTTTTTCT ATTGGCTAAT GCTTTAGCTT CTTTATATTG AATCGGCACA CAAGATAACA 15304 | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCTTTTTTCT ATTGGCTAAT GCTTTAGCTT CTTTATATTG AATCGGCACA CAAGATAACA 418 AATGTCTATT TAAT-CCCCC GTCCCACCTT GACCCCCACC TTGTTTATGT TTAAAAGGTT 15363 |||||||||| |||| ||||| || ||||||| |||||||||| |||||||||| ||||||| || AATGTCTATT TAATCCCCCC GTTCCACCTT GACCCCCACC TTGTTTATGT TTAAAAGCTT 478 GCTTACACTT ATTACAAAAT AGCAACACTA TTTTCTTTAT CGAAAGTCAT AAATTGCCAC 15423 |||||||||| ||||| |||| |||||||||| |||||||||| || ||||||| |||||||||| GCTTACACTT ATTAC-AAAT AGCAACACTA TTTTCTTTAT CGTAAGTCAT AAATTGCCAC 537 ACAATATAAG TTTTAGGGCG TTGATACTTC ACCTTCTCCC TTGTGAGAG 15472 |||||| ||| ||||||| || |||||||||| |||||||||| ||||| ||| ACAATAGAAG TTTTAGGTCG TTGATACTTC ACCTTCTCCC TTGTGGGAG 586 hqPGS_C06HBa0046G10.1-1+_SGN-E392581- (14886 15472) ******************************************************************************** EST sequence 14 -strand 273 n (File: SGN-E274195-) 1 GCTCTCGATG CATTACCGGT AATATCTAAT TCATTAATTG AAATTCCCTT TTTTCTATTG 61 GATAATGCTT CAGCTTCTTT ATATTGAATC GGCACACAAG ATAACAAATG TCTATTTAAT 121 CCCCCCGTTC CACCTTGACC CCCACCTTGT TTATGTTTAA AAGCATGCTT ACACTTATTA 181 CAAATAGCAA CACTATTTTC TTTATCGTAA GTCATAAATT GCCACACAAT AGAAGTTTTA 241 GGTCGTGGAT ACTTCACCTT CTCCCTTGTG GGA Predicted gene structure (within gDNA segment 13950 to 16098): Exon 1 15200 15471 ( 272 n); cDNA 2 273 ( 272 n); score: 0.928 MATCH C06HBa0046G10.1-1+ SGN-E274195- 0.928 272 0.996 C PGS_C06HBa0046G10.1-1+_SGN-E274195- (15200 15471) Alignment (genomic DNA sequence = upper lines): CTACCGATGC ACTACCGGTA ATATCAAATT CATCAATTGA AATTCCTTTT TTTCTATTGG 15259 || |||||| | |||||||| ||||| |||| ||| |||||| |||||| ||| |||||||||| CTCTCGATGC ATTACCGGTA ATATCTAATT CATTAATTGA AATTCCCTTT TTTCTATTGG 61 CTAATGCTTT AGCTTCTTTA TATTGAATCG GCACACAAGA TAACAAATGT CTATTTAAT- 15318 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ATAATGCTTC AGCTTCTTTA TATTGAATCG GCACACAAGA TAACAAATGT CTATTTAATC 121 CCCCCGTCCC ACCTTGACCC CCACCTTGTT TATGTTTAAA AGGTTGCTTA CACTTATTAC 15378 ||||||| || |||||||||| |||||||||| |||||||||| || |||||| |||||||||| CCCCCGTTCC ACCTTGACCC CCACCTTGTT TATGTTTAAA AGCATGCTTA CACTTATTAC 181 AAAATAGCAA CACTATTTTC TTTATCGAAA GTCATAAATT GCCACACAAT ATAAGTTTTA 15438 ||||||||| |||||||||| ||||||| || |||||||||| |||||||||| | |||||||| -AAATAGCAA CACTATTTTC TTTATCGTAA GTCATAAATT GCCACACAAT AGAAGTTTTA 240 GGGCGTTGAT ACTTCACCTT CTCCCTTGTG AGA 15471 || ||| ||| |||||||||| |||||||||| || GGTCGTGGAT ACTTCACCTT CTCCCTTGTG GGA 273 hqPGS_C06HBa0046G10.1-1+_SGN-E274195- (15200 15471) ******************************************************************************** EST sequence 13 -strand 263 n (File: SGN-E274025-) 1 CATTACCGGT AATATCTAAT TCATTAATTG AAATTCCCTT TTTTCTATTG GCTAATGCTT 61 TAGCTTCTTT ATATTGAATC GGCACACAAG ATAACAAATG TCTATTTAAT CCCCCCGTTC 121 CACCTTGACC CCCACCTTGT TTATGTTTAA AAGCTTGCTT ACACTTATTA CAAATAGCAA 181 CACTATTTTC TTTATCGTAA GTCATAAATT GCCACACAAT AGAAGTTTTA GGTCGTTGAT 241 ACTTCACCTT CTCCCTTGTG GGA Predicted gene structure (within gDNA segment 14257 to 16098): Exon 1 15209 15471 ( 263 n); cDNA 1 263 ( 263 n); score: 0.949 MATCH C06HBa0046G10.1-1+ SGN-E274025- 0.949 263 1.000 C PGS_C06HBa0046G10.1-1+_SGN-E274025- (15209 15471) Alignment (genomic DNA sequence = upper lines): CACTACCGGT AATATCAAAT TCATCAATTG AAATTCCTTT TTTTCTATTG GCTAATGCTT 15268 || ||||||| |||||| ||| |||| ||||| ||||||| || |||||||||| |||||||||| CATTACCGGT AATATCTAAT TCATTAATTG AAATTCCCTT TTTTCTATTG GCTAATGCTT 60 TAGCTTCTTT ATATTGAATC GGCACACAAG ATAACAAATG TCTATTTAAT -CCCCCGTCC 15327 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| | TAGCTTCTTT ATATTGAATC GGCACACAAG ATAACAAATG TCTATTTAAT CCCCCCGTTC 120 CACCTTGACC CCCACCTTGT TTATGTTTAA AAGGTTGCTT ACACTTATTA CAAAATAGCA 15387 |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| | |||||||| CACCTTGACC CCCACCTTGT TTATGTTTAA AAGCTTGCTT ACACTTATTA C-AAATAGCA 179 ACACTATTTT CTTTATCGAA AGTCATAAAT TGCCACACAA TATAAGTTTT AGGGCGTTGA 15447 |||||||||| |||||||| | |||||||||| |||||||||| || ||||||| ||| |||||| ACACTATTTT CTTTATCGTA AGTCATAAAT TGCCACACAA TAGAAGTTTT AGGTCGTTGA 239 TACTTCACCT TCTCCCTTGT GAGA 15471 |||||||||| |||||||||| | || TACTTCACCT TCTCCCTTGT GGGA 263 hqPGS_C06HBa0046G10.1-1+_SGN-E274025- (15209 15471) ******************************************************************************** EST sequence 2 -strand 756 n (File: SGN-E339247-) 1 CTGCAGAACG TCTCTCCCAA ATATATTGCC CTGCAGATCA CAAATTGTAA AGGGGAAAAC 61 AGCAATAACT AATCACAACA AATCCACCAT ATAAGCAGTG ATTGCACATC TCCCATTATT 121 TTTTGTTCGA ATATATGCAC AAAATTTTTG ACAACATATA CTCCATTTCA AAAAGAATAA 181 CGGATATCAG AGTACGAAGG CTCAAACCAT TCGATCTCAT TGCAATTTGT AGATCACTTC 241 TTTCAAATTT TAAAAATGAA ATGTCCACAT TTAAAAACTG CATAAACAGT AGTAATACTA 301 GAAGTTTCAA CTTTTGTAAT TTTAAATTAT TACAAATTTA CAAGAAGAAT CTATTGGACC 361 TCAAAAAAGA AATACTACAA GAACAACAAC AATCCCAGTG TGATCCCACA AGTGTTGGTG 421 AGGAGGTAGG ATGTAGGATG TATGCAGACC TTACCCCTAC TTTGGGGTAG AGAGGTTGTT 481 TCTGATTGGC TCGAAAATGT GAAAGAATGA CAAAAGTCCC ACATTGGTGG TTAATAAGAT 541 GGGTGGACTC TTTATAAGGC TTGAGTTAGC TTTTGGGGTG TGAGTTAGGC CTAAGACCTA 601 GTAGACCCAA AATCAAACAT TGCTCACACA CCAGATGCTA AGCACTGGCA TGAGGTGGTG 661 TGTTAAAGAA TGACAAAAGT CTCACATCGG TGGTTAATGA GATGGGTGAA CTCCTTATAA 721 GACTTGGACA ATTATCCTCC CTTTGAGCTA GCTTTT Predicted gene structure (within gDNA segment 22710 to 16078): Exon 1 21813 21726 ( 88 n); cDNA 382 466 ( 85 n); score: 0.676 Intron 1 21725 20622 (1104 n); Pd: 0.355 (s: 0.66), Pa: 0.788 (s: 0) Exon 2 20621 20606 ( 16 n); cDNA 467 482 ( 16 n); score: 0.562 Intron 2 20605 19441 (1165 n); Pd: 0.502 (s: 0), Pa: 0.000 (s: 0) Exon 3 19440 19428 ( 13 n); cDNA 483 495 ( 13 n); score: 0.615 Intron 3 19427 17605 (1823 n); Pd: 0.958 (s: 0), Pa: 0.000 (s: 0.83) Exon 4 17604 17541 ( 64 n); cDNA 496 560 ( 65 n); score: 0.836 Intron 4 17540 17348 ( 193 n); Pd: 0.000 (s: 0.85), Pa: 0.000 (s: 0.98) Exon 5 17347 17308 ( 40 n); cDNA 561 600 ( 40 n); score: 0.975 Intron 5 17307 17266 ( 42 n); Pd: 0.000 (s: 0.98), Pa: 0.000 (s: 0.81) Exon 6 17265 17111 ( 155 n); cDNA 601 756 ( 156 n); score: 0.852 MATCH C06HBa0046G10.1-1- SGN-E339247- 0.798 376 0.497 C PGS_C06HBa0046G10.1-1-_SGN-E339247- (21813 21726,20621 20606,19440 19428,17604 17541,17347 17308,17265 17111) Alignment (genomic DNA sequence = upper lines): AACAACAACA TATCCAATAT AATCTCACAA GTGGCATCTT AGAAGGGTAG AGTCTAGTGC 21754 |||||||||| ||| | | ||| ||||| ||| | || | ||||| | ||| AACAACAACA ATCCCAGTGT GATCCCACAA GTG-TTGGTG AGGA-GGTAG GATGTAGGAT 439 ATACGCAGAC CTTACCC-TA CCTTGGGAAG TAGATGCATC TTCTTGATAC CATCTACGAA 21695 || |||||| ||||||| || | ||||| GTATGCAGAC CTTACCCCTA CTTTGGG--. .......... .......... .......... 466 ATTCTGGTTC CATTAATGAA CTTATTTATT TTTTGTTATG ATGAAGCAAG AGAATTTGAC 21635 .......... .......... .......... .......... .......... .......... 466 ACCATCTATG AGATTCTCTT ACTTCATCAA AACAAAAAAT TATACCATAA AAAGAAATTG 21575 .......... .......... .......... .......... .......... .......... 466 AAAAAATTAT AGTTGATGGA AATATGATAG ATAGTGAAAA GCTGAACATG AGGTATTCTC 21515 .......... .......... .......... .......... .......... .......... 466 ACTTGCAACA AGAATTGTAC ATGTGGTCGA TGAGACAGAT ACCTACTCTC AATGGTGCGG 21455 .......... .......... .......... .......... .......... .......... 466 TTTTAGTAGA TTCCAGAATA TCAAAGGTGT AAGTAAATTA AGTTATCATT TGGAGGAGGA 21395 .......... .......... .......... .......... .......... .......... 466 AGTCTATGAC CCAAGGATAA AAGCCACAAG GCAGTTTTTA TTGCTTTATT CTTGAAAATG 21335 .......... .......... .......... .......... .......... .......... 466 AAAGAGTTCT AAATATCAAA AACTGTGACC TATTAGCTTT GATGGATGCT TTTATAAACA 21275 .......... .......... .......... .......... .......... .......... 466 CATTGCCAAG GTCATCAATG ACATATGACA CAAAAATGCT TACGGTGAAG GAGGAAATTC 21215 .......... .......... .......... .......... .......... .......... 466 TCGACGTTTT CAAAGGGAAA ATTAGGGTAT TAACTGAAGA TGTGTGGAAA TAAAATATTA 21155 .......... .......... .......... .......... .......... .......... 466 GGATTAGACG ATAGTCTTTA CAACATTGCG GCCTAGATGA TTGATCAATG CTATGCTTCC 21095 .......... .......... .......... .......... .......... .......... 466 ATTTTGAAAC AGCAAAGTAT TGTATTGATC CAAGAATTAC AGGAATCTCT CAAAGTGCTA 21035 .......... .......... .......... .......... .......... .......... 466 TGAAAGTTGA GACCAGAAGC CATTTGGATA GAGAGAGAGA AAGAGAGAGA CAGAGACAGA 20975 .......... .......... .......... .......... .......... .......... 466 GAGAGAGACA GAGAGAGAGA GGATTCTTGA ATATACAAAT ATCATTTTTT TGATAAGGTA 20915 .......... .......... .......... .......... .......... .......... 466 TTCTCGAATA TAATATTACC TGTCATAAAT AATTACTGTG TCAGCAGTCG CAAGATTAAT 20855 .......... .......... .......... .......... .......... .......... 466 TCCTAAACCT CCAGCCCTAG TGGATAGAAG GAAGCAGAAT CTTGAAGAAT TCTTTGCATT 20795 .......... .......... .......... .......... .......... .......... 466 AAATCTGTCA ATTCGGATTT GTCGTTCTGC CCCAGGAACT TTTCCATCAA TCCGTTCGTA 20735 .......... .......... .......... .......... .......... .......... 466 GTGCCATTTC TAAAAACATT AACAGGTAAA AATGCATCAT ATTTTGAGAT AATAAAGATG 20675 .......... .......... .......... .......... .......... .......... 466 AATACCAAAT CAGGTTTAAA ATAATATAAT GAACTAGTAT CTTTACCTTA TAGGTACAGT 20615 ||| || .......... .......... .......... .......... .......... ...GTAGAGA 473 AGTCTTCTAG CAAGTCGAGC ATATGCTGAA ATTGTGAATA TATAAGAACT CTGTGACCTT 20555 || | | GGTTGTTTC. .......... .......... .......... .......... .......... 482 GGTCTTTAAG CTTAACCATC ATCTTATCTA GAAGTTGTAA TTTGCCAGAA GATTCCAGCA 20495 .......... .......... .......... .......... .......... .......... 482 GCTGTCTGCC AAATCAAAAT GATCCACAGT GCAGATAAGA TAGGGTAGTT GAAAGAACTC 20435 .......... .......... .......... .......... .......... .......... 482 AAGAATAAAC ATTTTTTACT TCAAAAAAAG CTCTAAGCAC ACCCTTCTTG CAGGCAGGAT 20375 .......... .......... .......... .......... .......... .......... 482 CATCAACGAT CTTACTAGGC AACATTAGTG CTTAGGAAAT TCATAAAACT CCAGTCTCCT 20315 .......... .......... .......... .......... .......... .......... 482 AAAAGAATTT TCTCACTTTA GGCCCTAATG ATCATAAGAA ACTCCTTTGA CTGCAATTTC 20255 .......... .......... .......... .......... .......... .......... 482 CAGATTAAAA CAAAAGATTC TCTCCAAATA AAATGGTATC ATAAAAAAAA TGAAGAATCG 20195 .......... .......... .......... .......... .......... .......... 482 TGCTAAACAT TTTCTTCTAG GGAAATATAT CACTTTGACG TGGAGACATT AAAAGAAAAG 20135 .......... .......... .......... .......... .......... .......... 482 TTGAAAAGGG AGCAACATGT TGATATTGCG GACTCTTCAA AAATATTGTT GGTCGAGTGT 20075 .......... .......... .......... .......... .......... .......... 482 TGGATCCTCC CAAAGTAGTG CATTTTTTGA GGATCCAACA CGGGTGCAAC AATTTGGGGA 20015 .......... .......... .......... .......... .......... .......... 482 ATCCAAGCAA CATAGCAAAA CGCTACTTAT TTAACAAGAC ATCGCTCCGC CACCATCATG 19955 .......... .......... .......... .......... .......... .......... 482 AAAGGGAGAA GAGAACCACT CTCACTTAAC ATATCGCCCC TCCATCCAAC ATTAATGGCC 19895 .......... .......... .......... .......... .......... .......... 482 CTACTCAGAG TAGCACCACT ACCACCTTCC CTTAGCCTCA TAGCCCTTAT CATATTCTCA 19835 .......... .......... .......... .......... .......... .......... 482 CAACTGCCAA CCCCTGTCCT TTTCCCTGAA CAACTTCTCC CCTTCTCATT GGCTTGGTCA 19775 .......... .......... .......... .......... .......... .......... 482 CAGCTCTCAC TAACACACCT CTTCGACCCA CAATATGCTG TTCTTCTACC TGCTATAGTT 19715 .......... .......... .......... .......... .......... .......... 482 GCTGACAATG TTTCCGAATT TTATTAGTGA AAAGATGGGA AACCACCCAC CTTTCTCTGC 19655 .......... .......... .......... .......... .......... .......... 482 CTCCATCTCG GTCACAGATA ACAACAACCC CCACCCTAAT TGTACCCCAG ATTCCCTTCA 19595 .......... .......... .......... .......... .......... .......... 482 TAAATCATGT ATCTCATATT CCACCCCTAC CAGAGATGGT GGCCCCTTCA CCCTCTGCAT 19535 .......... .......... .......... .......... .......... .......... 482 GCTATATTCC TTCGTTAACA AAGAAAAAAG ATAAGACTTC AAACATACCT TCTTAATTAT 19475 .......... .......... .......... .......... .......... .......... 482 TTTTTATTTT ATTCTTTTCT TTTAAAAAAA AACTTGATTT TCTGCAGGCA AGAAATCAAT 19415 ||||| || | .......... .......... .......... ....TGATTG GCTCGAA... .......... 495 GAAATTTATT ATAAGATAAG AAGTGTACTT GGTAAACTCG TTAGTATCCT CTGGTTCAAC 19355 .......... .......... .......... .......... .......... .......... 495 TCCTTCTAAC ATAAATGGAT GACAGCAGAG CTTGCGCAGT TCCATAACCA CATTTATGAG 19295 .......... .......... .......... .......... .......... .......... 495 GGAAATCTGA TAGGCAAGTA TCAACCAAGG TCAGAATTCC TGTTACAGAA CAATAAGCAT 19235 .......... .......... .......... .......... .......... .......... 495 ATATCAAACA AGAAGAAGCT GGAGCACCTG AGCACCTCCC TTGCGAGCCA GTATCTGGAA 19175 .......... .......... .......... .......... .......... .......... 495 GTTACGTGTC AAAATTGCTT TGTAATATTC TTTCTGCTTG CTACTCAATT CCACTCGCAA 19115 .......... .......... .......... .......... .......... .......... 495 AATGAGCTCC TTTTTTGGAG GCAGCTCCTT CATGACATCT TTCTTGACCC CTGCAGCCAA 19055 .......... .......... .......... .......... .......... .......... 495 CAAAACACAC TTACACCAAA GCTCAAGAGT TCCGCATAAA GATAAACACA ATCATGTTTC 18995 .......... .......... .......... .......... .......... .......... 495 TTGAAGCACT GCCCTAGCCC ATAACTATAA GCTTCACTCT GGAACTGCAA GTACATGTAA 18935 .......... .......... .......... .......... .......... .......... 495 TTTGAAAGAA GTACATAAAT TTCAGAAACT TAATCCATTT GACCCGGTAC AGCATTGTAC 18875 .......... .......... .......... .......... .......... .......... 495 TATGTACCTA TGAGTTACAC AGGAATAAGC ATGCATGTGG GGCTTCTGGA GAACGATTAC 18815 .......... .......... .......... .......... .......... .......... 495 ATTCATCTAA TGATGGTGTG GTTGATTTGA GAGGAGGACA ACAAGAAGAA TTTCCAACAA 18755 .......... .......... .......... .......... .......... .......... 495 CAAATGATAT AAGGAGAACT TGGCATCTAC TTCTTGCTTG TTTGACCACC CATCCTCAAA 18695 .......... .......... .......... .......... .......... .......... 495 GGTGTACAAG ATAAAGTATT TAATGGAAAA ACGAACAATA AAAATAAGTT TAATATGATA 18635 .......... .......... .......... .......... .......... .......... 495 TAACTATGGC ATCTGTAATG CGGTTAGCAA AGGCTTGCTT AACAGAGACC TACAATTTAG 18575 .......... .......... .......... .......... .......... .......... 495 GAATCAAACA AAGATGTATG AGTAGCATAG TGAAACACCC TGATAGTGCC TACTTCCACA 18515 .......... .......... .......... .......... .......... .......... 495 AATCAATGTA AACAATAACT ACTTATACAT CTACTGACAA ATCACGAAAT AAAAGCTTGA 18455 .......... .......... .......... .......... .......... .......... 495 GATTTGTACT TCTCAGCAGA TGGGGTGCCA GCATTTTATG AAGTCTTGAA ATCTGTTCTT 18395 .......... .......... .......... .......... .......... .......... 495 CCTGACTAAT ATCCTCAAAT TCCTGTTGGA ATTCCTCCAA GCTTCCAAAC TACAATCAAG 18335 .......... .......... .......... .......... .......... .......... 495 TTCCATATAA AAATTTCACA TCATAACAAA TCAACACGTA AATTGGTTAG AGACGAATGC 18275 .......... .......... .......... .......... .......... .......... 495 TTCAAAAAAT CTGTGGTAGC ACTAACCTTC CCAGCATCAA GGAAGTGCAT AAGCATGAAA 18215 .......... .......... .......... .......... .......... .......... 495 AGTTCATCCA GATTGTTCTG AAAGATGGGG AGAAAAGGAG TGTCATAACC AATAGTTTGC 18155 .......... .......... .......... .......... .......... .......... 495 AGATCACTGT TAGTGGCAGA TAACAGATGA TGGTGATGAG ATGACAATAG ACGATACTCA 18095 .......... .......... .......... .......... .......... .......... 495 TGTTAATAAC AATTATTATT AATATCATAA TAATTAGTTC TACCACTAGT ACTAGTATTA 18035 .......... .......... .......... .......... .......... .......... 495 TTATTATCAG TACAACTACG ACTACTATCA TTGGCACTAA CATCATTATT ACGACAATCC 17975 .......... .......... .......... .......... .......... .......... 495 TAATCATCCT GTTTTTATTA TTGCTGTAAC AATATTAATA TGAGAAGTGC TGTAGGTGAG 17915 .......... .......... .......... .......... .......... .......... 495 TTAAATGTTT TAGATAAATG ACAAAATTCT ACATTCCAAT TATCAGATAC CCTGAAAGGA 17855 .......... .......... .......... .......... .......... .......... 495 TGATAAATGA CAATATTAAT TTTTTTTTAT ACAGGTAAAA TTAATGAATG ACTGTATTCT 17795 .......... .......... .......... .......... .......... .......... 495 CTTTGGAGAG AAACAAAAGA CCAAGTGACC CGCCTACATC ACTACTACCA GTCAATTGGC 17735 .......... .......... .......... .......... .......... .......... 495 AATAAGTTAT GATTGAGATG TTTGCTTTTC ACTTTATTGA TACTTGGGAG CATCTTCAGC 17675 .......... .......... .......... .......... .......... .......... 495 TACCTTGGCA TATGGGAAGT TAATGTTGGA CATTCTGTGA AGGGCCTTTA AAGTCAAATT 17615 .......... .......... .......... .......... .......... .......... 495 CGGGGTGCTT TATGTTAAAG AATGGCAAAA GTCCCACATC GATGGTTAAT GAGATGGGT- 17556 |||| |||| |||| ||||| ||||||||| | |||||||| |||||||| .......... AATGTGAAAG AATGACAAAA GTCCCACATT GGTGGTTAAT AAGATGGGTG 545 AACTCCTTAT AAGGCCGACC TAATTTCACA TGGTATCAGA GCAGGGCCCG TCTCACCCGA 17496 |||| |||| ||||| GACTCTTTAT AAGGC..... .......... .......... .......... .......... 560 TGTTGGGATC CCCAAAAATC AAAAATGTCC ACGCACCAAA TACTAAGTAC TGGCCGCGAG 17436 .......... .......... .......... .......... .......... .......... 560 GTGGGCTGTT AAAAAATGAC AAAAGTCCCA CATAAGAAAT TAATGAGATG GATGGACTCC 17376 .......... .......... .......... .......... .......... .......... 560 TTATAAGGTT TGGGCAATCC TCATCCCTTT GAGCTAGCTT TTGGGGTGTG AGTTAGGCCT 17316 || ||| |||||| |||||||||| |||||||||| .......... .......... ........TT GAGTTAGCTT TTGGGGTGTG AGTTAGGCCT 592 AAGACCTAAC TTCACATGGT ACCAAAGCAG GACCCGTCTC ACCCTATGTT GGAACCCCAA 17256 |||||||| | | ||||| AAGACCTA.. .......... .......... .......... .......... GTAGACCCAA 610 AATCAAA-AT TGTCCACGCA CCAGATGCTA AGCACTGGGC GTGATGTGGG GTGTTAAAGA 17197 ||||||| || || ||| || |||||||||| |||||| ||| ||| |||| |||||||||| AATCAAACAT TGCTCACACA CCAGATGCTA AGCACT-GGC ATGAGGTGGT GTGTTAAAGA 669 ATGACAAAAG TCTCACATCG GTGGTTAATG AGATGGGTGG AAAACTTATA AGGCTTGGGC 17137 |||||||||| |||||||||| |||||||||| ||||||||| | |||||| || ||||| | ATGACAAAAG TCTCACATCG GTGGTTAATG AGATGGGTGA ACTCCTTATA AGACTTGGAC 729 AA-TCTCCTC CCTTTGAGTT AGCTTTT 17111 || | ||||| |||||||| | ||||||| AATTATCCTC CCTTTGAGCT AGCTTTT 756 hqPGS_C06HBa0046G10.1-1-_SGN-E339247- (17604 17541,17347 17308,17265 17111) Total number of EST alignments reported: 16 ________________________________________________________________________________ Predicted gene locations (3) in segment 1 to 22710: PGL 1 (- strand): 14422 13217 AGS-1 (14422 13593,13519 13217) SCR (e 0.901 d 0.000 a 0.972,e 0.911) Exon 1 14422 13593 ( 830 n); score: 0.901 Intron 1 13592 13520 ( 73 n); Pd: 0.000 Pa: 0.972 Exon 2 13519 13217 ( 303 n); score: 0.911 PGS (13524 13217) SGN-E392585- PGS (13477 13217) SGN-E392580- PGS (13888 13593,13519 13492) SGN-E320889+ PGS (14194 13593) SGN-E251178+ PGS (14240 13618) SGN-E262684+ PGS (14194 13652) SGN-E251179+ PGS (14422 13861) SGN-E238157+ PGS (14368 13931) SGN-E279072+ 3-phase translation of AGS-1 (-strand): . . . . . . 14422 TGGAATTCATTTTACGAAATGTTTTCAGTTGCTTACAAATATAGAAGACCCATACAAATG W N S F Y E M F S V A Y K Y R R P I Q M G I H F T K C F Q L L T N I E D P Y K C E F I L R N V F S C L Q I - K T H T N . . . . . . 14362 TGTTTAATGTTCATAATGCTGACCCATTAGATAGAATTTGTGATGAAGATTGGGAAGAAA C L M F I M L T H - I E F V M K I G K K V - C S - C - P I R - N L - - R L G R N V F N V H N A D P L D R I C D E D W E E . . . . . . 14302 CTAAAGACCTATTACAATTTTTAAGACTTTTTTATGATGCAACAATCATGTTTTCTGGAA L K T Y Y N F - D F F M M Q Q S C F L E - R P I T I F K T F L - C N N H V F W N T K D L L Q F L R L F Y D A T I M F S G . . . . . . 14242 TTTATTATCCTACTATTTTATCAGTATTAATAAATATTTGTGCTCTTACAATTCAATTTT F I I L L F Y Q Y - - I F V L L Q F N F L L S Y Y F I S I N K Y L C S Y N S I F I Y Y P T I L S V L I N I C A L T I Q F . . . . . . 14182 CTAAATATAAAAAAATAGATAAATTTAAAGTTGCAATTGAAGGTATGATTGAAAAATTAA L N I K K - I N L K L Q L K V - L K N - - I - K N R - I - S C N - R Y D - K I K S K Y K K I D K F K V A I E G M I E K L . . . . . . 14122 AAAAATATTTTTTTCTTATTCCTCAAATTTATTTAACTGCTACTTTACTTTCATCCTGAT K N I F F L F L K F I - L L L Y F H P D K I F F S Y S S N L F N C Y F T F I L I K K Y F F L I P Q I Y L T A T L L S S - . . . . . . 14062 TATAAATTACGAGGTGTGCAGGGCCTTATTGACACTTTTTGTGAAACTTAAGAAATTTTA Y K L R G V Q G L I D T F C E T - E I L I N Y E V C R A L L T L F V K L K K F Y L - I T R C A G P Y - H F L - N L R N F . . . . . . 14002 CCTAAAGAAATTCCAAATTGTGAAACGTGTAAGTCTAGCATAAAAGTAGAAGCAAAAATT P K E I P N C E T C K S S I K V E A K I L K K F Q I V K R V S L A - K - K Q K F T - R N S K L - N V - V - H K S R S K N . . . . . . 13942 TTGTATAAAAAATATAGAACTAGTGAAAATATTGAAGGAGAAGTTGGTCAAACTTCTAAT L Y K K Y R T S E N I E G E V G Q T S N C I K N I E L V K I L K E K L V K L L I F V - K I - N - - K Y - R R S W S N F - . . . . . . 13882 CAAGATATTGATTTTGAAGTTCGAATTTCAACTTATATGAGAGGTTTTCTTTGTCTTAAT Q D I D F E V R I S T Y M R G F L C L N K I L I L K F E F Q L I - E V F F V L I S R Y - F - S S N F N L Y E R F S L S - . . . . . . 13822 TCTACTAACCGTGATGATTTAGAAGAATATATTAATCAATCTTTAGAAGTATTTGAAATC S T N R D D L E E Y I N Q S L E V F E I L L T V M I - K N I L I N L - K Y L K S F Y - P - - F R R I Y - S I F R S I - N . . . . . . 13762 AAAGATGGCAATGAAGATTTATTAGGATGTGTAGTAGGCGAACCGTTGCATTTCCAACTT K D G N E D L L G C V V G E P L H F Q L K M A M K I Y - D V - - A N R C I S N F Q R W Q - R F I R M C S R R T V A F P T . . . . . . 13702 TGAGCAAAATGATTTGTGATGTTCTAGTTATTCAAGCTTCATCAGTTGCATTGGATGCTG - A K - F V M F - L F K L H Q L H W M L E Q N D L - C S S Y S S F I S C I G C C L S K M I C D V L V I Q A S S V A L D A . . . . . : . 13642 CTTTCAGTGCGGCAAGGTTTCAAATTGGTGATCATCGTCATTCAATTTGC : TGATGATAAA L S V R Q G F K L V I I V I Q F A : D D K F Q C G K V S N W - S S S F N L : L M I N A F S A A R F Q I G D H R H S I C : - - - . . . . . . 13509 TTAGAGTCACAAGAGTTTCGGGCAAGTTTGCCAACACCGAAGGATATCACCATCGATATG L E S Q E F R A S L P T P K D I T I D M - S H K S F G Q V C Q H R R I S P S I - I R V T R V S G K F A N T E G Y H H R Y . . . . . . 13449 ATGCGACAATTAGAATTAAATTTTAAAGGAGAAAAAAGATATTTTAGATTACATAAGAGA M R Q L E L N F K G E K R Y F R L H K R C D N - N - I L K E K K D I L D Y I R E D A T I R I K F - R R K K I F - I T - E . . . . . . 13389 ACTAATTGAAAAAAAACCCTTCCTAGAAGGTGGCTGCGTAAAATTAGTTACTCACCTAAT T N - K K T L P R R W L R K I S Y S P N L I E K K P F L E G G C V K L V T H L I N - L K K N P S - K V A A - N - L L T - . . . . . . 13329 TTTTTTAACAGATATTAGTTTTAAGTATTAAAACTTATTGAAACAGAACCCTTCCTAGAA F F N R Y - F - V L K L I E T E P F L E F L T D I S F K Y - N L L K Q N P S - K F F - Q I L V L S I K T Y - N R T L P R . . . . . . 13269 GGTGGCTGCGTAGATTAGTTACTCCACTAATATTTGTTAATTGTAAATTCTAA G G C V D - L L H - Y L L I V N S V A A - I S Y S T N I C - L - I L R W L R R L V T P L I F V N C K F - Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-1-_PGL-1_AGS-1_PPS_1 (14378 14064) (frame '0'; 312 bp, 104 residues) 1 KTHTNVFNVH NADPLDRICD EDWEETKDLL QFLRLFYDAT IMFSGIYYPT ILSVLINICA 61 LTIQFSKYKK IDKFKVAIEG MIEKLKKYFF LIPQIYLTAT LLSS- >C06HBa0046G10.1-1-_PGL-1_AGS-1_PPS_2 (14011 13700) (frame '1'; 309 bp, 103 residues) 1 EILPKEIPNC ETCKSSIKVE AKILYKKYRT SENIEGEVGQ TSNQDIDFEV RISTYMRGFL 61 CLNSTNRDDL EEYINQSLEV FEIKDGNEDL LGCVVGEPLH FQL- >C06HBa0046G10.1-1-_PGL-1_AGS-1_PPS_3 (13675 13593,13519 13381) (frame '1'; 219 bp, 73 residues) 1 LFKLHQLHWM LLSVRQGFKL VIIVIQFADD KLESQEFRAS LPTPKDITID MMRQLELNFK 61 GEKRYFRLHK RTN- PGL 2 (+ strand): 14672 16126 AGS-1 (14672 15918,16084 16126) SCR (e 0.905 d 0.000 a 0.000,e 0.860) Exon 1 14672 15918 (1247 n); score: 0.905 Intron 1 15919 16083 ( 165 n); Pd: 0.000 Pa: 0.000 Exon 2 16084 16126 ( 43 n); score: 0.860 PGS (14672 15446) SGN-E378787- PGS (14832 15472) SGN-E392586- PGS (14886 15472) SGN-E392581- PGS (15200 15471) SGN-E274195- PGS (15209 15471) SGN-E274025- PGS (15304 15918,16084 16126) SGN-E349085+ 3-phase translation of AGS-1 (+strand): . . . . . . 14672 AGCGTTTGTATTGTTAGATGCATTATCTAATGCAACAGACATTACTTTATCTGTAACCAT S V C I V R C I I - C N R H Y F I C N H A F V L L D A L S N A T D I T L S V T M R L Y C - M H Y L M Q Q T L L Y L - P . . . . . . 14732 GTAATAATCTAAAACACTTAAAATTTTTGAAGCTAAGTATGCACCAGTTTTTTCTCTACA V I I - N T - N F - S - V C T S F F S T - - S K T L K I F E A K Y A P V F S L H C N N L K H L K F L K L S M H Q F F L Y . . . . . . 14792 CATAGTGTATAACTTAATATTCTTTTTTGCAAATTCCAATTATGATCAATCCAATGAGTT H S V - L N I L F C K F Q L - S I Q - V I V Y N L I F F F A N S N Y D Q S N E L T - C I T - Y S F L Q I P I M I N P M S . . . . . . 14852 GAAACAGTTAAATAATCATTTCCATTGACACTACGACCCATATCCGTAGTTATAGACACT E T V K - S F P L T L R P I S V V I D T K Q L N N H F H - H Y D P Y P - L - T L - N S - I I I S I D T T T H I R S Y R H . . . . . . 14912 CTACTATATAATATGCTAAATATACAACGAAGAAATTAACAATGTTTACCTTGAAATTCA L L Y N M L N I Q R R N - Q C L P - N S Y Y I I C - I Y N E E I N N V Y L E I Q S T I - Y A K Y T T K K L T M F T L K F . . . . . . 14972 AAAACATCATGTTTAACAGTATTTCTTGAAAACCTCTATAATTCGGATTATAAGTTTGTT K T S C L T V F L E N L Y N S D Y K F V K H H V - Q Y F L K T S I I R I I S L L K N I M F N S I S - K P L - F G L - V C . . . . . . 15032 AAATTTATTCAATAAAATCGGGATATGAAGTAAAACTATAAGGCAAACTACAAACAGAAA K F I Q - N R D M K - N Y K A N Y K Q K N L F N K I G I - S K T I R Q T T N R N - I Y S I K S G Y E V K L - G K L Q T E . . . . . . 15092 CCATTTTAGCTAAATTTTCGCGATCAAATTCCTTATTATATTTACGTTGTGTTATTGGAC P F - L N F R D Q I P Y Y I Y V V L L D H F S - I F A I K F L I I F T L C Y W T T I L A K F S R S N S L L Y L R C V I G . . . . . . 15152 CACCGGGGCTAGAAAGATCTAATGTTCCTTGAACTATGTGAGAAGCCCCTACCGATGCAC H R G - K D L M F L E L C E K P L P M H T G A R K I - C S L N Y V R S P Y R C T P P G L E R S N V P - T M - E A P T D A . . . . . . 15212 TACCGGTAATATCAAATTCATCAATTGAAATTCCTTTTTTTCTATTGGCTAATGCTTTAG Y R - Y Q I H Q L K F L F F Y W L M L - T G N I K F I N - N S F F S I G - C F S L P V I S N S S I E I P F F L L A N A L . . . . . . 15272 CTTCTTTATATTGAATCGGCACACAAGATAACAAATGTCTATTTAATCCCCCGTCCCACC L L Y I E S A H K I T N V Y L I P R P T F F I L N R H T R - Q M S I - S P V P P A S L Y - I G T Q D N K C L F N P P S H . . . . . . 15332 TTGACCCCCACCTTGTTTATGTTTAAAAGGTTGCTTACACTTATTACAAAATAGCAACAC L T P T L F M F K R L L T L I T K - Q H - P P P C L C L K G C L H L L Q N S N T L D P H L V Y V - K V A Y T Y Y K I A T . . . . . . 15392 TATTTTCTTTATCGAAAGTCATAAATTGCCACACAATATAAGTTTTAGGGCGTTGATACT Y F L Y R K S - I A T Q Y K F - G V D T I F F I E S H K L P H N I S F R A L I L L F S L S K V I N C H T I - V L G R - Y . . . . . . 15452 TCACCTTCTCCCTTGTGAGAGGTATAAGGAGTGGACGTCCACAATTTTGCTCCGTCGAAT S P S P L - E V - G V D V H N F A P S N H L L P C E R Y K E W T S T I L L R R I F T F S L V R G I R S G R P Q F C S V E . . . . . . 15512 TTATAGTGTCATTTGGAACTTCTGTCACCGGACTAGTAGGTGTATCTTCTAAATCCTTTT L - C H L E L L S P D - - V Y L L N P F Y S V I W N F C H R T S R C I F - I L F F I V S F G T S V T G L V G V S S K S F . . . . . . 15572 CTCACAAATCAGCTTCTACTTCTTCACCTGAATTTTCATCAACATTAGGATTAATATTAC L T N Q L L L L H L N F H Q H - D - Y Y S Q I S F Y F F T - I F I N I R I N I T S H K S A S T S S P E F S S T L G L I L . . . . . . 15632 CATAACTTCGTTCTAAACTTTGATGATCGAGTTGAACATTTGAATTAATATCATAAGGAG H N F V L N F D D R V E H L N - Y H K E I T S F - T L M I E L N I - I N I I R S P - L R S K L - - S S - T F E L I S - G . . . . . . 15692 TATCAACATAAGTAGAATCATTTGGGTTAAATCTAACTCTACACGTTGATTTATATGAAG Y Q H K - N H L G - I - L Y T L I Y M K I N I S R I I W V K S N S T R - F I - S V S T - V E S F G L N L T L H V D L Y E . . . . . . 15752 TACCACCACTTTTACTACTCTTTTTTTCTTTATTATTTTTTTTACTAAAATTAAATATTT Y H H F Y Y S F F L Y Y F F Y - N - I F T T T F T T L F F F I I F F T K I K Y F V P P L L L L F F S L L F F L L K L N I . . . . . . 15812 TAAAAATTCCACTTTGTTCGTTATCTTGTTCTTTTTCTTTTCCTTTACCGATATTTTTTT - K F H F V R Y L V L F L F L Y R Y F F K N S T L F V I L F F F F S F T D I F F L K I P L C S L S C S F S F P L P I F F . . . . . : . 15872 TTGCTAGAACTCATACTTAATTATATATAAAATGTAATATAAAAATA : TTTATATATATAT L L E L I L N Y I - N V I - K Y : L Y I Y C - N S Y L I I Y K M - Y K N : I Y I Y I F A R T H T - L Y I K C N I K I : F I Y I . . . 16097 ATATATATATATATAAAATAATATGAAAAT I Y I Y I K - Y E N Y I Y I - N N M K Y I Y I Y K I I - K Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-1+_PGL-2_AGS-1_PPS_1 (15336 15563) (frame '2'; 225 bp, 75 residues) 1 PPPCLCLKGC LHLLQNSNTI FFIESHKLPH NISFRALILH LLPCERYKEW TSTILLRRIY 61 SVIWNFCHRT SRCIF- AGS-2 (15304 15918,16086 16124) SCR (e 0.861 d 0.000 a 0.000,e 0.821) Exon 1 15304 15918 ( 615 n); score: 0.861 Intron 1 15919 16085 ( 167 n); Pd: 0.000 Pa: 0.000 Exon 2 16086 16124 ( 39 n); score: 0.821 PGS (15304 15918,16086 16124) SGN-E350209+ 3-phase translation of AGS-2 (+strand): . . . . . . 15304 AAATGTCTATTTAATCCCCCGTCCCACCTTGACCCCCACCTTGTTTATGTTTAAAAGGTT K C L F N P P S H L D P H L V Y V - K V N V Y L I P R P T L T P T L F M F K R L M S I - S P V P P - P P P C L C L K G . . . . . . 15364 GCTTACACTTATTACAAAATAGCAACACTATTTTCTTTATCGAAAGTCATAAATTGCCAC A Y T Y Y K I A T L F S L S K V I N C H L T L I T K - Q H Y F L Y R K S - I A T C L H L L Q N S N T I F F I E S H K L P . . . . . . 15424 ACAATATAAGTTTTAGGGCGTTGATACTTCACCTTCTCCCTTGTGAGAGGTATAAGGAGT T I - V L G R - Y F T F S L V R G I R S Q Y K F - G V D T S P S P L - E V - G V H N I S F R A L I L H L L P C E R Y K E . . . . . . 15484 GGACGTCCACAATTTTGCTCCGTCGAATTTATAGTGTCATTTGGAACTTCTGTCACCGGA G R P Q F C S V E F I V S F G T S V T G D V H N F A P S N L - C H L E L L S P D W T S T I L L R R I Y S V I W N F C H R . . . . . . 15544 CTAGTAGGTGTATCTTCTAAATCCTTTTCTCACAAATCAGCTTCTACTTCTTCACCTGAA L V G V S S K S F S H K S A S T S S P E - - V Y L L N P F L T N Q L L L L H L N T S R C I F - I L F S Q I S F Y F F T - . . . . . . 15604 TTTTCATCAACATTAGGATTAATATTACCATAACTTCGTTCTAAACTTTGATGATCGAGT F S S T L G L I L P - L R S K L - - S S F H Q H - D - Y Y H N F V L N F D D R V I F I N I R I N I T I T S F - T L M I E . . . . . . 15664 TGAACATTTGAATTAATATCATAAGGAGTATCAACATAAGTAGAATCATTTGGGTTAAAT - T F E L I S - G V S T - V E S F G L N E H L N - Y H K E Y Q H K - N H L G - I L N I - I N I I R S I N I S R I I W V K . . . . . . 15724 CTAACTCTACACGTTGATTTATATGAAGTACCACCACTTTTACTACTCTTTTTTTCTTTA L T L H V D L Y E V P P L L L L F F S L - L Y T L I Y M K Y H H F Y Y S F F L Y S N S T R - F I - S T T T F T T L F F F . . . . . . 15784 TTATTTTTTTTACTAAAATTAAATATTTTAAAAATTCCACTTTGTTCGTTATCTTGTTCT L F F L L K L N I L K I P L C S L S C S Y F F Y - N - I F - K F H F V R Y L V L I I F F T K I K Y F K N S T L F V I L F . . . . . . 15844 TTTTCTTTTCCTTTACCGATATTTTTTTTTGCTAGAACTCATACTTAATTATATATAAAA F S F P L P I F F F A R T H T - L Y I K F L F L Y R Y F F L L E L I L N Y I - N F F F S F T D I F F C - N S Y L I I Y K . . : . . . . 15904 TGTAATATAAAAATA : TATATATATATATATATATATATATAAAATAATATGAAA C N I K I : Y I Y I Y I Y I - N N M K V I - K Y : I Y I Y I Y I Y K I I - M - Y K N : I Y I Y I Y I Y I K - Y E Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-1+_PGL-2_AGS-2_PPS_1 (15336 15563) (frame '0'; 225 bp, 75 residues) 1 PPPCLCLKGC LHLLQNSNTI FFIESHKLPH NISFRALILH LLPCERYKEW TSTILLRRIY 61 SVIWNFCHRT SRCIF- PGL 3 (- strand): 17604 17111 AGS-1 (17604 17541,17347 17308,17265 17111) SCR (e 0.836 d 0.000 a 0.000,e 0.975 d 0.000 a 0.000,e 0.852) Exon 1 17604 17541 ( 64 n); score: 0.836 Intron 1 17540 17348 ( 193 n); Pd: 0.000 Pa: 0.000 Exon 2 17347 17308 ( 40 n); score: 0.975 Intron 2 17307 17266 ( 42 n); Pd: 0.000 Pa: 0.000 Exon 3 17265 17111 ( 155 n); score: 0.852 PGS (17604 17541,17347 17308,17265 17111) SGN-E339247- 3-phase translation of AGS-1 (-strand): . . . . . . 17604 TATGTTAAAGAATGGCAAAAGTCCCACATCGATGGTTAATGAGATGGGTAACTCCTTATA Y V K E W Q K S H I D G - - D G - L L I M L K N G K S P T S M V N E M G N S L - C - R M A K V P H R W L M R W V T P Y . : . . . . : . 17544 AGGC : TTGAGCTAGCTTTTGGGGTGTGAGTTAGGCCTAAGACCTA : GGAACCCCAAAATCAA R : L E L A F G V - V R P K T - : E P Q N Q G : L S - L L G C E L G L R P : R N P K I K K A : - A S F W G V S - A - D L : G T P K S . . . . . . 17249 AATTGTCCACGCACCAGATGCTAAGCACTGGGCGTGATGTGGGGTGTTAAAGAATGACAA N C P R T R C - A L G V M W G V K E - Q I V H A P D A K H W A - C G V L K N D K K L S T H Q M L S T G R D V G C - R M T . . . . . . 17189 AAGTCTCACATCGGTGGTTAATGAGATGGGTGGAAAACTTATAAGGCTTGGGCAATCTCC K S H I G G - - D G W K T Y K A W A I S S L T S V V N E M G G K L I R L G Q S P K V S H R W L M R W V E N L - G L G N L . . 17129 TCCCTTTGAGTTAGCTTTT S L - V S F P F E L A F L P L S - L Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Mon Aug 28 21:40:40 2006 ________________________________________________________________________________ Sequence 2: C06HBa0046G10.1-2, from 1 to 6980, both strands analyzed. ... started at: Mon Aug 28 21:40:40 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 5 ... matches indexed, elapsed seconds = 5 HitsTableSize = 1 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 0 ******************************************************************************** EST sequence 1 +strand 599 n (File: SGN-E345050+) 1 AAAAGCTCAC TTGTTCTTCT AGGGTTTCAC AAGATCTCTT TTGCCTCTGC AAAACACGAG 61 CGAATTGCGA TTTCTAGGGT TACGATTTGT GTTTGTTCGG GTGTTTGATG GTCCGAGGAG 121 TGATTTGAGG GTATAAATCT TGATTTTTTC TGGAAGATTT TGAAGAGAGA AAGGAAACGA 181 TGGCGCCTTT TGACAAAGAG CTTGAAGAGC AGATTGCTGT TGCTGGGAAC AAGCTGATTG 241 AGCCTCCTTC TTCACTTGAA GAACTCCTCC GTTTGCTTGA CCAAGTAGAG TCTTCCCTGT 301 CTAAAGTGGA GCAGTCACCA GCGAAATCAA TGCATGATGC ACTTTCTCCA TTGATGAAGG 361 CATTGGTTGC AAATGATCTC TTGAGGCATT CTGATGTTGA TGTGAAGGTC GCAGTTGCTT 421 CGTGTATTAG TGAGATAACT AGAATTACTG CACCAGACGC TCCATATGAT GACGACAAAA 481 TGAAGGATAT CTTTCAATTA ATTGTGTCGT CTTTTGAGAA TCTGGATGAC CAGTCTAGCA 541 GATCTTATAA TAAGAGGGTG ATGATCCTTG AGACAGTGGC CAAGGTCAGG TCATGTGTT Predicted gene structure (within gDNA segment 545 to 5783): Exon 1 1145 1425 ( 281 n); cDNA 1 281 ( 281 n); score: 1.000 Intron 1 1426 4782 (3357 n); Pd: 0.996 (s: 1.00), Pa: 0.863 (s: 1.00) Exon 2 4783 4986 ( 204 n); cDNA 282 485 ( 204 n); score: 1.000 Intron 2 4987 5059 ( 73 n); Pd: 0.999 (s: 1.00), Pa: 0.932 (s: 1.00) Exon 3 5060 5173 ( 114 n); cDNA 486 599 ( 114 n); score: 1.000 MATCH C06HBa0046G10.1-2+ SGN-E345050+ 1.000 599 1.000 C PGS_C06HBa0046G10.1-2+_SGN-E345050+ (1145 1425,4783 4986,5060 5173) Alignment (genomic DNA sequence = upper lines): AAAAGCTCAC TTGTTCTTCT AGGGTTTCAC AAGATCTCTT TTGCCTCTGC AAAACACGAG 1204 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAAGCTCAC TTGTTCTTCT AGGGTTTCAC AAGATCTCTT TTGCCTCTGC AAAACACGAG 60 CGAATTGCGA TTTCTAGGGT TACGATTTGT GTTTGTTCGG GTGTTTGATG GTCCGAGGAG 1264 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGAATTGCGA TTTCTAGGGT TACGATTTGT GTTTGTTCGG GTGTTTGATG GTCCGAGGAG 120 TGATTTGAGG GTATAAATCT TGATTTTTTC TGGAAGATTT TGAAGAGAGA AAGGAAACGA 1324 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATTTGAGG GTATAAATCT TGATTTTTTC TGGAAGATTT TGAAGAGAGA AAGGAAACGA 180 TGGCGCCTTT TGACAAAGAG CTTGAAGAGC AGATTGCTGT TGCTGGGAAC AAGCTGATTG 1384 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGCGCCTTT TGACAAAGAG CTTGAAGAGC AGATTGCTGT TGCTGGGAAC AAGCTGATTG 240 AGCCTCCTTC TTCACTTGAA GAACTCCTCC GTTTGCTTGA CGTAAGCTTT TTTGTTTATT 1444 |||||||||| |||||||||| |||||||||| |||||||||| | AGCCTCCTTC TTCACTTGAA GAACTCCTCC GTTTGCTTGA C......... .......... 281 TTGGTTGTGA ATTCTGTTTT TGTATAATCG GTATGATATT TTTTTTAGAT TTTTATGTTT 1504 .......... .......... .......... .......... .......... .......... 281 AGGTTTTTTT TTTGTTATCT TGAACTATTT CATACCATTT TTGGGCTACT TCGAATTGTT 1564 .......... .......... .......... .......... .......... .......... 281 TTAGATGTTT GTTTAAGTAA TTCTCGGGTT GAGTTTCTGC TTTTGTATGA TGCTCCGCTT 1624 .......... .......... .......... .......... .......... .......... 281 TACTTTAGAG CTAATTAGGG CTAGATTCAA AGAATAAAAC TTGGAAGCCT ACAAAATAAT 1684 .......... .......... .......... .......... .......... .......... 281 TGAACTATGT GTTGACTCCA GGGCATTGAA CCTGAGCATC CCACATCTGG GAGATATGTG 1744 .......... .......... .......... .......... .......... .......... 281 TACACCCTTG ACTGGTGACC TCTGCTCTAG GTTTTGTCAA AGGGTGTTCA AAATTCTATA 1804 .......... .......... .......... .......... .......... .......... 281 TATACAAATA AAAACTAGTA TTTAAGGCTA ATGATGTTCG GCTGATTACC CTAGTACCGC 1864 .......... .......... .......... .......... .......... .......... 281 CGCAGCTGTG CCCTTGTTGA TTACCCTAGC ACCGCCATAG CTATGCCCTT ATTGATTACC 1924 .......... .......... .......... .......... .......... .......... 281 CTAGCACTGC TGTAGCTCTG CCCTTGTTGA TTACCCTAGC ACCGCCGTAG CTATGCCCTT 1984 .......... .......... .......... .......... .......... .......... 281 GTTGATTACC CTAGCACCGC TGTAGCTCTG CCCTTGTTGA TTACCCTAGA TCTGCCCTTG 2044 .......... .......... .......... .......... .......... .......... 281 TTGAACACTG TTATCAACTT CTAATTTATT TTGATAAGGA AGTAAACATT ATAAACTATC 2104 .......... .......... .......... .......... .......... .......... 281 TTTTGGTTGA AAGCTTAACT TTTATTCAGC CAGTATGGAA ATAGTACACC AGTTAGCTAT 2164 .......... .......... .......... .......... .......... .......... 281 TCTAATTTCT CACTCTTTTA CAATCCTCAT TAAGCTAGCT ATTCTATCAG TTTCTACTCT 2224 .......... .......... .......... .......... .......... .......... 281 ACAATTCTAG CTATCTGTCT AATCTTTGAA GCCCCCCCCC CCCCCACCAC ACCACACCAC 2284 .......... .......... .......... .......... .......... .......... 281 ACACTTACAC TTGGTCTGGT GTTGTCCTTT TGTGTGAAGT TGGGGATGAT TCCTCTGGAT 2344 .......... .......... .......... .......... .......... .......... 281 CTATGCTTGC ATTCCTGCTG GTGTTGTTGA AATATTCTAG CATTCTGCTC AATCCAGACA 2404 .......... .......... .......... .......... .......... .......... 281 TGGTAAATGC ATGCATTGAA CAGCCAGCCT AGCATGTGTA ATTCTGCTTT CCTTTGTTTC 2464 .......... .......... .......... .......... .......... .......... 281 ACTCTGTGCG TCAACTATTG GATCTCAGCC TCCCAATTTT AGTGATTCAA AATATGATTT 2524 .......... .......... .......... .......... .......... .......... 281 AAAAAAGCCC TTCTTGATTG GTAACAGCCT TACCAATATC TTCACAAGTT AGTGGTAGGT 2584 .......... .......... .......... .......... .......... .......... 281 CTACAGACAC ACCACCCTCC CTAGACCCAA CTTGTGGGAT TACAGTGGTA ATGTTGTTGT 2644 .......... .......... .......... .......... .......... .......... 281 CGTTTCTCTT TCTTGTGATG TATACGATGT TATCCCCCAG GACAGTATTT TGATTCTTAC 2704 .......... .......... .......... .......... .......... .......... 281 TCTATATAGA GCCCTTGAAT ACTGTTATTA GCTTTTGATT TGTTTTGATA GTGAGGTAGA 2764 .......... .......... .......... .......... .......... .......... 281 AACTGACTTC TTTTAATTAT TCTAAATATG ATTAAAAATC TTTCTTTATT TGTTATCCTC 2824 .......... .......... .......... .......... .......... .......... 281 CACCCTGGAT GATTGCCATA TGTTCCCTCT CCTCTTGTTT GATGCAAGTC CTTTACTATT 2884 .......... .......... .......... .......... .......... .......... 281 CTTCAAATTT AATTCTTAAT GGCCTACCCG CTGTTTCAGT TTGCATTGAG TTGAATTATC 2944 .......... .......... .......... .......... .......... .......... 281 CTGCTTAATG AAAGTATTGC TTTTAAAATT CATGCTTTTC CTAATGTTTC TTTTAAATTT 3004 .......... .......... .......... .......... .......... .......... 281 ATTTTGATAT TTGTTGTCTC TGCCTATTTG GAAATATTTT GGCATGTCTT TTTTATAAGT 3064 .......... .......... .......... .......... .......... .......... 281 AATTTCTGTA TTCTGGTAAT TTTTCATTTT TTCCTGTTAA CTGGTGGTCT CTGTTTAGAG 3124 .......... .......... .......... .......... .......... .......... 281 TCTGGTTTAA ACTATCTTGA AGCATTATGG TTTGCAATTG ATGGGCATTG CCACTAGGGC 3184 .......... .......... .......... .......... .......... .......... 281 CACTCTTTTT TCATGACTCC CCTGTGGGAT GTGGAATATT ACTGGGTATG TTGTTGCATG 3244 .......... .......... .......... .......... .......... .......... 281 TGATGTATAC ATTAGTCGCA ACTTTTTGTT TGGGTCCTAG AGTTTTGTGG TTTGCTAGTG 3304 .......... .......... .......... .......... .......... .......... 281 AGACATATGA GCATGTCCAT AGTTAAATAA TTTGAACAGG ATGTAGTCTG TGTAATCTTG 3364 .......... .......... .......... .......... .......... .......... 281 ACATCTCCTG GCGGAGCTTT TTATTTTTGA TAATTGTTTC AAATGATGGT ATCTGAAAAT 3424 .......... .......... .......... .......... .......... .......... 281 TCCAGAGAGA TAATTGGGTT GGCGGGTAAT AAGTACACCA TTCTTTTTAT TTGATAAGTG 3484 .......... .......... .......... .......... .......... .......... 281 CCTGACAATT TGTAGGAAAG GGAAAATGAA AAAAATGGAA GAAGTAGTAT ATACTCTTGG 3544 .......... .......... .......... .......... .......... .......... 281 AATCTCTGGG TATTAAATAA CAGTCAATAT TTATGCTGAA AGATAGCAAG GCTGTGGGCA 3604 .......... .......... .......... .......... .......... .......... 281 TGCTGTGTGT TTCCCCTGTG AGGCTCTTCT AGTTGTACTT GAAAATCCCG TTGGCTTTTC 3664 .......... .......... .......... .......... .......... .......... 281 CTGGAGGGTT TCTCAAGGAG CAGAGTATGG GCAATATTAG TTTTCTTTCT GAAGTGGTGA 3724 .......... .......... .......... .......... .......... .......... 281 TTTTCTTATG CAAATGGTTT ATTGATTTAA CCCCCCATCT TAATTGAGAA GCCACAATGA 3784 .......... .......... .......... .......... .......... .......... 281 AATAAGTTTC CTTTTGTGGG TGTCTTCCCC TAGTCAAAGT TAAAATGCAT AGATTTGTGT 3844 .......... .......... .......... .......... .......... .......... 281 TTAACAATCT CATAAATTAA TTGTCATTTT ACTTTTTAGA GAAAATCTGA CTGTGCATCT 3904 .......... .......... .......... .......... .......... .......... 281 TCGTATTGCT GGTTAAAGAA CGATAAACAG TGACAGATAC TAATTTGGAA CCATGAGTTT 3964 .......... .......... .......... .......... .......... .......... 281 CCTGGTATTT CCTTGATAAC AAGCTGATGC AGACCACCTG TTTTTGGTGA TAAAATTGAT 4024 .......... .......... .......... .......... .......... .......... 281 ACTTTATGAA TACGTCCTTT AGATTATTGT TTAGAAGATT TGGTTTGTGT GATTTTATGC 4084 .......... .......... .......... .......... .......... .......... 281 TGCCATAATT CTTTTGGGAA AATACAAAAA CCTTTAGCCT GTATGGATAA AATTGGGGCC 4144 .......... .......... .......... .......... .......... .......... 281 AGCTGATTGT CATCACTTAA TCGATGTGCG AGACTCTGAG GTACCTTAAC ATGATCTGTA 4204 .......... .......... .......... .......... .......... .......... 281 GTAAAAAGGA TCAAGTTGAC TGTTATTCCC CTTTTTCTAA TCAATGTGCG AGACTCTGAG 4264 .......... .......... .......... .......... .......... .......... 281 GTACCTTAAC ATGATCTGTA GTAAAAAGGA TCAAGTTGGC TGTTATTCCC CTTTTTCTTT 4324 .......... .......... .......... .......... .......... .......... 281 AAAGTGGGTC ATTGACTTCT AGATAGGAGA TCTTTTTTCT TCTATAAATT TGTTTGGAAT 4384 .......... .......... .......... .......... .......... .......... 281 TTAACCCTGC AGTAGTATGG TTGGTTACAT GGAGAAAAGG AAAAGCTAGA GTTTTTGAAC 4444 .......... .......... .......... .......... .......... .......... 281 TTAAGGAAGG ACAGTGTAAT TAACCATGGG TCGGAGTTTA TATTCTTGCT TAGTTTTAGT 4504 .......... .......... .......... .......... .......... .......... 281 GGTGGAGACT TGGTTGACTT AGCAGGTCCT TTACACTTGC TGACTTTTTT TTTTGATGTA 4564 .......... .......... .......... .......... .......... .......... 281 CTCTTTTCCC ATCATTGCAG ATATTTCATA CATTACCCAT ATATCAATAG ATACTGTTGG 4624 .......... .......... .......... .......... .......... .......... 281 TATAACAAAA CAGAAGTTAT TTTGAACCGT TTGTTTTGGC AGATTTTTGG TTTCACTGTT 4684 .......... .......... .......... .......... .......... .......... 281 AATTTTGCAA TTAACATATA ATGTTTCATT TGCTGAAACT CATGCACGGT CTTTTTCTTG 4744 .......... .......... .......... .......... .......... .......... 281 CCTAATCTTA ATATACTTTT GGTTTTTTCT GCTTTAAGCA AGTAGAGTCT TCCCTGTCTA 4804 || |||||||||| |||||||||| .......... .......... .......... ........CA AGTAGAGTCT TCCCTGTCTA 303 AAGTGGAGCA GTCACCAGCG AAATCAATGC ATGATGCACT TTCTCCATTG ATGAAGGCAT 4864 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGGAGCA GTCACCAGCG AAATCAATGC ATGATGCACT TTCTCCATTG ATGAAGGCAT 363 TGGTTGCAAA TGATCTCTTG AGGCATTCTG ATGTTGATGT GAAGGTCGCA GTTGCTTCGT 4924 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTGCAAA TGATCTCTTG AGGCATTCTG ATGTTGATGT GAAGGTCGCA GTTGCTTCGT 423 GTATTAGTGA GATAACTAGA ATTACTGCAC CAGACGCTCC ATATGATGAC GACAAAATGA 4984 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTATTAGTGA GATAACTAGA ATTACTGCAC CAGACGCTCC ATATGATGAC GACAAAATGA 483 AGGTATGAGA AATCTGCTTT CTCATTCTCC TTCCTTCTAT CAGTCCTTCA ATAACATTCC 5044 || AG........ .......... .......... .......... .......... .......... 485 ATTTATTTGA TACAGGATAT CTTTCAATTA ATTGTGTCGT CTTTTGAGAA TCTGGATGAC 5104 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GATAT CTTTCAATTA ATTGTGTCGT CTTTTGAGAA TCTGGATGAC 530 CAGTCTAGCA GATCTTATAA TAAGAGGGTG ATGATCCTTG AGACAGTGGC CAAGGTCAGG 5164 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGTCTAGCA GATCTTATAA TAAGAGGGTG ATGATCCTTG AGACAGTGGC CAAGGTCAGG 590 TCATGTGTT 5173 ||||||||| TCATGTGTT 599 hqPGS_C06HBa0046G10.1-2+_SGN-E345050+ (1145 1425,4783 4986,5060 5173) Total number of EST alignments reported: 1 ________________________________________________________________________________ Predicted gene locations (1) in segment 1 to 6980: PGL 1 (+ strand): 1145 5173 AGS-1 (1145 1425,4783 4986,5060 5173) SCR (e 1.000 d 0.996 a 0.863,e 1.000 d 0.999 a 0.932,e 1.000) Exon 1 1145 1425 ( 281 n); score: 1.000 Intron 1 1426 4782 (3357 n); Pd: 0.996 Pa: 0.863 Exon 2 4783 4986 ( 204 n); score: 1.000 Intron 2 4987 5059 ( 73 n); Pd: 0.999 Pa: 0.932 Exon 3 5060 5173 ( 114 n); score: 1.000 PGS (1145 1425,4783 4986,5060 5173) SGN-E345050+ 3-phase translation of AGS-1 (+strand): . . . . . . 1145 AAAAGCTCACTTGTTCTTCTAGGGTTTCACAAGATCTCTTTTGCCTCTGCAAAACACGAG K S S L V L L G F H K I S F A S A K H E K A H L F F - G F T R S L L P L Q N T S K L T C S S R V S Q D L F C L C K T R . . . . . . 1205 CGAATTGCGATTTCTAGGGTTACGATTTGTGTTTGTTCGGGTGTTTGATGGTCCGAGGAG R I A I S R V T I C V C S G V - W S E E E L R F L G L R F V F V R V F D G P R S A N C D F - G Y D L C L F G C L M V R G . . . . . . 1265 TGATTTGAGGGTATAAATCTTGATTTTTTCTGGAAGATTTTGAAGAGAGAAAGGAAACGA - F E G I N L D F F W K I L K R E R K R D L R V - I L I F S G R F - R E K G N D V I - G Y K S - F F L E D F E E R K E T . . . . . . 1325 TGGCGCCTTTTGACAAAGAGCTTGAAGAGCAGATTGCTGTTGCTGGGAACAAGCTGATTG W R L L T K S L K S R L L L L G T S - L G A F - Q R A - R A D C C C W E Q A D - M A P F D K E L E E Q I A V A G N K L I . . . . . : . 1385 AGCCTCCTTCTTCACTTGAAGAACTCCTCCGTTTGCTTGAC : CAAGTAGAGTCTTCCCTGT S L L L H L K N S S V C L T : K - S L P C A S F F T - R T P P F A - : P S R V F P V E P P S S L E E L L R L L D : Q V E S S L . . . . . . 4802 CTAAAGTGGAGCAGTCACCAGCGAAATCAATGCATGATGCACTTTCTCCATTGATGAAGG L K W S S H Q R N Q C M M H F L H - - R - S G A V T S E I N A - C T F S I D E G S K V E Q S P A K S M H D A L S P L M K . . . . . . 4862 CATTGGTTGCAAATGATCTCTTGAGGCATTCTGATGTTGATGTGAAGGTCGCAGTTGCTT H W L Q M I S - G I L M L M - R S Q L L I G C K - S L E A F - C - C E G R S C F A L V A N D L L R H S D V D V K V A V A . . . . . . 4922 CGTGTATTAGTGAGATAACTAGAATTACTGCACCAGACGCTCCATATGATGACGACAAAA R V L V R - L E L L H Q T L H M M T T K V Y - - D N - N Y C T R R S I - - R Q N S C I S E I T R I T A P D A P Y D D D K . : . . . . . 4982 TGAAG : GATATCTTTCAATTAATTGTGTCGTCTTTTGAGAATCTGGATGACCAGTCTAGCA - R : I S F N - L C R L L R I W M T S L A E : G Y L S I N C V V F - E S G - P V - Q M K : D I F Q L I V S S F E N L D D Q S S . . . . . . 5115 GATCTTATAATAAGAGGGTGATGATCCTTGAGACAGTGGCCAAGGTCAGGTCATGTGTT D L I I R G - - S L R Q W P R S G H V I L - - E G D D P - D S G Q G Q V M C R S Y N K R V M I L E T V A K V R S C V Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-2+_PGL-1_AGS-1_PPS_1 (1288 1425,4783 4986,5060 5173) (frame '0'; 456 bp, 152 residues) 1 FFLEDFEERK ETMAPFDKEL EEQIAVAGNK LIEPPSSLEE LLRLLDQVES SLSKVEQSPA 61 KSMHDALSPL MKALVANDLL RHSDVDVKVA VASCISEITR ITAPDAPYDD DKMKDIFQLI 121 VSSFENLDDQ SSRSYNKRVM ILETVAKVRS CV ... finished at: Mon Aug 28 21:40:50 2006 ________________________________________________________________________________ Sequence 3: C06HBa0046G10.1-3, from 1 to 7638, both strands analyzed. ... started at: Mon Aug 28 21:40:50 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 7 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 4 ******************************************************************************** EST sequence 2 +strand 667 n (File: SGN-E389613+) 1 TACATTACTG CAATCTCCAA ATCCAGCCAT AGATCCTTAG CATTGGACCA TTGGTAAAAG 61 TATTTAGTTA CAGATCCTTT TTTGCCTTTG GCAGCACTAA CTTATATACC TAATTAATTT 121 TGCCCCACAA TATGAAATGA GATAGCTTGC ATGCAACGCG TACCTGCTAC CTTCCACCAT 181 CACAAATCCA TAACCTGATA GTAATGAGTC AATGGAACCT TGACGGTGTT AGGACCAAAA 241 ATAAGCAGGT GTAAACGCGG AAGCTAGCAA AGCAAACCTC AAAAGACCAC GAGTAAGAAG 301 ACAACGAGAA ATATACCAAA AGATACAGAG ATTTAACGTG GTTCGGTCAA TTGACCTACG 361 TCCACAAAGG AGATGAGCAA TCCATTATAA ATATGAGAAT ACAAAATACA GAGAGAAACA 421 ACCTCAACCA AATTCACTCA GAATACATGA GAGATTCACA CAAGTGATAA CGTATCAAGA 481 AGCTTGTGAC TCATACCTCA ACGCATTATG TATGTGCAAA TCACAGGAGG AATGTACATT 541 CATGATCAGT TAAAAGTTAT AATAGAACAG GCAGAATAAA AGCAAAATAG AACATGATAA 601 TAATTTGTTA GAAACATTCT TGTACTCATC CAAATTAGTT GCAATGATTA CCTATTAGTA 661 ATATGAG Predicted gene structure (within gDNA segment 1442 to 7638): Exon 1 3494 3535 ( 42 n); cDNA 184 220 ( 37 n); score: 0.762 Intron 1 3536 4959 (1424 n); Pd: 0.000 (s: 0.76), Pa: 0.431 (s: 0.86) Exon 2 4960 5218 ( 259 n); cDNA 221 478 ( 258 n); score: 0.898 MATCH C06HBa0046G10.1-3+ SGN-E389613+ 0.898 301 0.451 C PGS_C06HBa0046G10.1-3+_SGN-E389613+ (3494 3535,4960 5218) Alignment (genomic DNA sequence = upper lines): AACTCCATAG CCCTTAAAGT AATTTAGAGT CATAAGGAAC CTCTAACACC AAGCGAATTT 3553 || |||||| ||| | ||| ||| |||| || | ||||| || AAATCCATAA -CCTGATAGT AAT---GAGT CA-ATGGAAC CT........ .......... 220 TTCATTAGTA TCGTTATTTT TGAAGTGACT GTGTTCTCCA TGCGGCCCAT AACATGGGGG 3613 .......... .......... .......... .......... .......... .......... 220 CACTATGCTT AACCAATTTT TCAGTAGCCT CACTATTTTT CGAAGTGGAT CTGCTCTCTA 3673 .......... .......... .......... .......... .......... .......... 220 TGTGACCCGT AGCGTGGACG CACGTGCCAA GCAAATTTTT GAATAACTCT GCTATTTTTA 3733 .......... .......... .......... .......... .......... .......... 220 AATGGTTGTG CGTTGCGCTT AGCATATAGT GTGTGCGTAC CGCTCCAAGT CAAGGGTCTA 3793 .......... .......... .......... .......... .......... .......... 220 GATATTAAGA TTCTCTATTC ATTAAGTTAA GTCAATGTAT CGCTATAAAG TTTATCTTAG 3853 .......... .......... .......... .......... .......... .......... 220 ACGACCTATG ATTTATTTTT ATCTCAAATT ATCTCCTTTC CTTCAAGTCC GACTCCAAGA 3913 .......... .......... .......... .......... .......... .......... 220 CATGTTTCTT ATATCTAGCT TAAATCTAAA CAATTTCGAT CTCTAAGCAC GAATTCTTAA 3973 .......... .......... .......... .......... .......... .......... 220 CAAGATTTTA ACATCAAATC TCTCTCATAT CTAATGAAAA TAAGAATTCA TCCTACAGTT 4033 .......... .......... .......... .......... .......... .......... 220 CAAAAAAGGG ACTTACAGAG TTCCTATTTG AAGTTAGAGT TTCAGTTAGA GTGGTTCAAA 4093 .......... .......... .......... .......... .......... .......... 220 ATAAATAAAG CTACTCATTA TGATGGACTA CGTTCTTCCT CAAAATTTTA CCGAATTTTT 4153 .......... .......... .......... .......... .......... .......... 220 CATGAATTTC AAATTGATCT TTATTCCTTG TTGTTGAATT CCAATTCTTA TCATTGAGAT 4213 .......... .......... .......... .......... .......... .......... 220 TATAGAACTT CATTTTCATT CTTAAATTGT TGTCATGCTT ATGGCATCAC ATGAACCCTA 4273 .......... .......... .......... .......... .......... .......... 220 TTTAGTTGAG AATTGTTGAA AGTTTTTGCA TAATTACTAT CGTGCGCTAA CTTTTTATTT 4333 .......... .......... .......... .......... .......... .......... 220 AAAGTAAGTT TAAGTGTTTT AAGTTATCAT TGAGAAGAGT TTTTTAGAGA ATACTAATGA 4393 .......... .......... .......... .......... .......... .......... 220 TTCGAAATTC ACTATTTTGT ATTAAGAAAT ATTGTTAATT ATAGAAAAGT ATAATTAATT 4453 .......... .......... .......... .......... .......... .......... 220 TCAAGAGAAG TTTCAATCAG TAAAGCAAGA TCAGTTATTC CTCAAAAGGA TTTTTTTAGT 4513 .......... .......... .......... .......... .......... .......... 220 ATTGATTCAA TCTAGTAAGT ATTACCATAT GAGAATTAAT ATGTATGTTT GGAGTAGTAT 4573 .......... .......... .......... .......... .......... .......... 220 TGAACATCAT TTTGGATAAA AGTTAAGATA ACTTGAACCC CATTAATTAC ATTGTCAGCG 4633 .......... .......... .......... .......... .......... .......... 220 TAGATAGGAT CATATCGAAA TCTGAGTGAA AGTTCACGTG CCTCATTAAA GCTTTTAGTG 4693 .......... .......... .......... .......... .......... .......... 220 GATTCATAAG TTAGGTTGTG TTATATATTC CGACAAGGAA AAATTTTATT TTGAGCGAGG 4753 .......... .......... .......... .......... .......... .......... 220 TACAACCAAC AATAGTTGTA CGAGGGTCCA TTCATTTTTA ACACGTGAAT ATGTGGGGTC 4813 .......... .......... .......... .......... .......... .......... 220 AATTTGTTCT TTAAAGTCCG CCAAAGAAAT AAGAGGAGAG AGAATATAAA TTTTAATTGA 4873 .......... .......... .......... .......... .......... .......... 220 AAATTAAAAA GAAAACGTGG GATCCATGCC ATTTTCAATA TATATATATA TAATTAATTA 4933 .......... .......... .......... .......... .......... .......... 220 ATTACATTTT TCGTACAAAA AAGAAGTGAC CAATTGTTAG GACCGGAAAT AAGCAGGTGT 4993 ||| | |||||| |||| |||| |||||||||| .......... .......... ......TGA- C-GGTGTTAG GACCAAAAAT AAGCAGGTGT 252 AAATGCGGAA GCTAGCAAAA TAAATCTCGA AAGATCACGA GTAAAAAAAC AACGAGAAAT 5053 ||| |||||| ||||||||| ||| ||| | |||| ||||| |||| || || |||||||||| AAACGCGGAA GCTAGCAAAG CAAACCTCAA AAGACCACGA GTAAGAAGAC AACGAGAAAT 312 ATACCAAAAA ACACAAAGAT TTAACGTGGT TCGGTCAATC GACCTACGTC CACAAAGGAG 5113 ||||||||| | ||| |||| |||||||||| ||||||||| |||||||||| |||||||||| ATACCAAAAG ATACAGAGAT TTAACGTGGT TCGGTCAATT GACCTACGTC CACAAAGGAG 372 ATGAGCAATC CATTATAAAT ATGAGAGTAC AAAATACAGA AGGAAACAAC CTCAA-CTAA 5172 |||||||||| |||||||||| |||||| ||| |||||||||| |||||||| ||||| | || ATGAGCAATC CATTATAAAT ATGAGAATAC AAAATACAGA GAGAAACAAC CTCAACCAAA 432 TTCACTCAGA ATACATGGGA GGTTCACACA AGTGATAACG TATCAA 5218 |||||||||| ||||||| || | |||||||| |||||||||| |||||| TTCACTCAGA ATACATGAGA GATTCACACA AGTGATAACG TATCAA 478 hqPGS_C06HBa0046G10.1-3+_SGN-E389613+ (3494 3535,4960 5218) ******************************************************************************** EST sequence 11 -strand 780 n (File: SGN-E543331-) 1 CAACTACAAT AACAATGTTT TCGCGTTTAA ATTGTTAGAA CCGGAAATAA GCAGGGTAAA 61 CGCGGAAAGC TAGCAAAGCA AACCTCAAAA GACCACGAGT ATGAAAGACA ACGAGAAATA 121 TACCAAAAGA CACAAAGATT TAACGTGGTT CGGTCAATCG ACCTACGTCC ACAATGGAGA 181 TGAGCAATCC ACTATTAATA TGAGAGTACA AAATACAGAG AGAAACAACC TCAACCAATT 241 CACTAAGAAT ACACGGGAAC ATTTTCCTAC CAGAAAAAGA ATTAGTCAAT TCAAAACTTT 301 TTTCCTAAAA GAAAAACCTA TTTATGATAA GAAATTTAGG ACAAAATAAA ATCCAACATA 361 AATGATGTAA TTATACAATT CATATCATCA GACAATGGTT CATGGATCCA AAATCTTGAA 421 TGATTCCATT TTGGTTTGAG CTCAAACATC ACCATCTATA TAACTAAAAC TCGTCGAAAT 481 TGACTCAACC TGCTCATCTG ACACTTGAAA ATTCGAGACT CAAATTTAAT CATTAAGATG 541 AATTTTGTGA ATTTAAATAC CTTTTGCACG AAATGTACGC TGATAAAGCA TTTTAAGTGT 601 TTTTGAGGAG TAGCAGACGA AGCAATAGCG GATGGTAATG GTGATGGACA ATCATTGATC 661 CTTAACGAAG TTGCTCGAAT TCCTTCAGAA ATTTCCTCCA TAGTAAATTG TGATTTGATT 721 AATTATTGGC TCAAATAAAC AAGAAAATGG ATGAACAAGA ACTAATTAAG CATGTTTATG Predicted gene structure (within gDNA segment 3362 to 7638): Exon 1 4975 5191 ( 217 n); cDNA 40 257 ( 218 n); score: 0.894 Intron 1 5192 5327 ( 136 n); Pd: 0.000 (s: 0.90), Pa: 0.000 (s: 0.87) Exon 2 5328 5438 ( 111 n); cDNA 258 371 ( 114 n); score: 0.806 MATCH C06HBa0046G10.1-3+ SGN-E543331- 0.864 328 0.421 C PGS_C06HBa0046G10.1-3+_SGN-E543331- (4975 5191,5328 5438) Alignment (genomic DNA sequence = upper lines): ACCGGAAATA AGCAGGTGTA AATGCGG-AA GCTAGCAAAA TAAATCTCGA AAGATCACGA 5033 |||||||||| |||||| ||| || |||| || ||||||||| ||| ||| | |||| ||||| ACCGGAAATA AGCAGG-GTA AACGCGGAAA GCTAGCAAAG CAAACCTCAA AAGACCACGA 98 GTA-AAAAAA CAACGAGAAA TATACCAAAA AACACAAAGA TTTAACGTGG TTCGGTCAAT 5092 ||| ||| | |||||||||| |||||||||| ||||||||| |||||||||| |||||||||| GTATGAAAGA CAACGAGAAA TATACCAAAA GACACAAAGA TTTAACGTGG TTCGGTCAAT 158 CGACCTACGT CCACAAAGGA GATGAGCAAT CCATTATAAA TATGAGAGTA CAAAATACAG 5152 |||||||||| |||||| ||| |||||||||| ||| ||| || |||||||||| |||||||||| CGACCTACGT CCACAATGGA GATGAGCAAT CCACTATTAA TATGAGAGTA CAAAATACAG 218 AAGGAAACAA CCTCAACTAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTGATAACG 5212 | ||||||| ||||||| || |||||| ||| ||||| ||| AGAGAAACAA CCTCAACCAA TTCACTAAGA ATACACGGG. .......... .......... 257 TATCAAGCTT GTGACCCATA AATTCTCCCC CTAACCAAAA CTCTCAAAGC CATTAAGACT 5272 .......... .......... .......... .......... .......... .......... 257 ACATTGTGAA TGCTGGTTAA GTTAGAAGGA ACATTCCTCT ATTTATAGAA TTCTAAACAT 5332 ||||| .......... .......... .......... .......... .......... .....AACAT 262 TTTCCTACAA GAAAATGATT TAGTCAATCC AAAA-TTTTT TCCTAAAA-A GAAACCTATT 5390 |||||||| | ||||| || | |||||||| | |||| ||||| |||||||| | ||||||||| TTTCCTACCA GAAAAAGAAT TAGTCAATTC AAAACTTTTT TCCTAAAAGA AAAACCTATT 322 TATGATAAGA AATTTAGGGC -AAATAAAAC TCAACACCAA TAAGTGAAT 5438 |||||||||| |||||||| | |||||||| ||||| || | | ||| TATGATAAGA AATTTAGGAC AAAATAAAAT CCAACATAAA TGATGTAAT 371 hqPGS_C06HBa0046G10.1-3+_SGN-E543331- (4975 5191,5328 5438) ******************************************************************************** EST sequence 8 -strand 747 n (File: SGN-E211090-) 1 CCCATATCAA CGGCTGGATT CAAGCTCTGC CCACAGTCGT AAATAATATC AGATTGCAAA 61 ATGGTAGTCT CTCCGGTCAG GATATCAATC TCCACCTCAC TGACAGCAGC ACCAAAGTTC 121 AAATAACTCG TAAAATCAGA TTCTGGTACA TAATAAGAAT TTGCTGCTAA GTTTACTGAT 181 TCCATTTGTG CCTGCAAAAA GAAGGGGGTG GAGGGACAGA ACTGCAAACT CAGATATGTT 241 AGGACCGAAA ATAAGCAGGT GTAAACGCGG AAGCTAGCAA TACAAACCTC GAAAGACCAC 301 GAGTAAGAAG ACAACGAGAA ATATATCAAA AGACACAAAG ATTTAACGTG GTTCGGTCAA 361 TCAACCTACG TCCACAAAGG AGATGAGCAA TCCACTATAA ATATAAGAGT ACAAAATACA 421 GAGAGAAACA ACCTCAACCA ATTCACTCAG AATACATGGG AGGTTCACAC AAGTGATAAC 481 ATATCAAGCT TGTGACCCAC AGATTCTCCC TCTAACCAAA ACTCTCAAAG CCTGTAAGAC 541 TACATTGTGA ATGCTGATTA AGTTAAAAGG AATATTCATC TATTTATAGA GTCCTAAACC 601 TTTTCCTACA AGAAAAGGAT TAGTCAATTC AAAACCTTTT CCTAAAAGGA AAACCTATTT 661 ATGGTAAGAA ATCAGGGCAA ATAAAATCCA ACAAGATACT TAAAAGATGA GCAAGAGTGG 721 CTAATGGATT TAAATTAACA AATTGTT Predicted gene structure (within gDNA segment 1134 to 7638): Exon 1 4957 5425 ( 469 n); cDNA 227 693 ( 467 n); score: 0.897 MATCH C06HBa0046G10.1-3+ SGN-E211090- 0.897 469 0.628 C PGS_C06HBa0046G10.1-3+_SGN-E211090- (4957 5425) Alignment (genomic DNA sequence = upper lines): AAGTGACCAA TTGTTAGGAC CGGAAATAAG CAGGTGTAAA TGCGGAAGCT AGCAAAATAA 5016 || | | | ||||||||| || ||||||| |||||||||| ||||||||| ||||| | || AACTCA-GAT ATGTTAGGAC CGAAAATAAG CAGGTGTAAA CGCGGAAGCT AGCAATACAA 285 ATCTCGAAAG ATCACGAGTA AAAAAACAAC GAGAAATATA CCAAAAAACA CAAAGATTTA 5076 | |||||||| | |||||||| | || ||||| |||||||||| ||||| ||| |||||||||| ACCTCGAAAG ACCACGAGTA AGAAGACAAC GAGAAATATA TCAAAAGACA CAAAGATTTA 345 ACGTGGTTCG GTCAATCGAC CTACGTCCAC AAAGGAGATG AGCAATCCAT TATAAATATG 5136 |||||||||| ||||||| || |||||||||| |||||||||| ||||||||| ||||||||| ACGTGGTTCG GTCAATCAAC CTACGTCCAC AAAGGAGATG AGCAATCCAC TATAAATATA 405 AGAGTACAAA ATACAGAAGG AAACAACCTC AACTAATTCA CTCAGAATAC ATGGGAGGTT 5196 |||||||||| ||||||| | |||||||||| ||| |||||| |||||||||| |||||||||| AGAGTACAAA ATACAGAGAG AAACAACCTC AACCAATTCA CTCAGAATAC ATGGGAGGTT 465 CACACAAGTG ATAACGTATC AAGCTTGTGA CCCATAAATT CTCCCCCTAA CCAAAACTCT 5256 |||||||||| ||||| |||| |||||||||| |||| | ||| ||||| |||| |||||||||| CACACAAGTG ATAACATATC AAGCTTGTGA CCCACAGATT CTCCCTCTAA CCAAAACTCT 525 CAAAGCCATT AAGACTACAT TGTGAATGCT GGTTAAGTTA GAAGGAACAT TCCTCTATTT 5316 ||||||| | |||||||||| |||||||||| | |||||||| |||||| || || ||||||| CAAAGCCTGT AAGACTACAT TGTGAATGCT GATTAAGTTA AAAGGAATAT TCATCTATTT 585 ATAGAATTCT AAACATTTTC CTACAAGAAA ATGATTTAGT CAATCCAAAA TTTTTTCCTA 5376 ||||| | || |||| ||||| |||||||||| | || ||||| |||| ||||| |||||||| ATAGAGTCCT AAACCTTTTC CTACAAGAAA AGGA-TTAGT CAATTCAAAA CCTTTTCCTA 644 AAAAG-AAAC CTATTTATGA TAAGAAATTT AGGGCAAATA AAACTCAACA 5425 ||| | |||| ||||||||| ||||||| | |||||||||| ||| ||||| AAAGGAAAAC CTATTTATGG TAAGAAA-TC AGGGCAAATA AAATCCAACA 693 hqPGS_C06HBa0046G10.1-3+_SGN-E211090- (4957 5425) ******************************************************************************** EST sequence 1 +strand 302 n (File: SGN-E370622+) 1 TTTTTTTTTT TGATAACCTA GAAATATGCC TATGACCCGC CCCTTTGGAC TAATCACAAC 61 CTTCTAAACT CGGTGGATAA TGTTAGGACC GAAAATAAGC AGGTGTAAAC GCGGAAGCTA 121 GCAACGCAAA CCTCAAAATA CCACGAGTAA GACGACAACG AGAAATATAC CAAAAGACAC 181 AAAGATTTAA CGTGGTTCGG TCAATCGACC TACGTCCACA AAGGAGATGA GCAATCTACT 241 ATAAATATGA GAGTACAAAA TACAGAGAGA AACAACCTCA ACCAATTCAC TCGGAATACA 301 TG Predicted gene structure (within gDNA segment 2902 to 6123): Exon 1 4960 5189 ( 230 n); cDNA 74 302 ( 229 n); score: 0.900 PPA cDNA 11 1 MATCH C06HBa0046G10.1-3+ SGN-E370622+ 0.900 230 0.762 C PGS_C06HBa0046G10.1-3+_SGN-E370622+ (4960 5189) Alignment (genomic DNA sequence = upper lines): TGACCAATTG TTAGGACCGG AAATAAGCAG GTGTAAATGC GGAAGCTAGC AAAATAAATC 5019 || || || ||||||||| |||||||||| ||||||| || |||||||||| || ||| | TGGATAA-TG TTAGGACCGA AAATAAGCAG GTGTAAACGC GGAAGCTAGC AACGCAAACC 132 TCGAAAGATC ACGAGTAAAA AAACAACGAG AAATATACCA AAAAACACAA AGATTTAACG 5079 || ||| | | |||||||| | |||||||| |||||||||| ||| |||||| |||||||||| TCAAAATACC ACGAGTAAGA CGACAACGAG AAATATACCA AAAGACACAA AGATTTAACG 192 TGGTTCGGTC AATCGACCTA CGTCCACAAA GGAGATGAGC AATCCATTAT AAATATGAGA 5139 |||||||||| |||||||||| |||||||||| |||||||||| |||| | ||| |||||||||| TGGTTCGGTC AATCGACCTA CGTCCACAAA GGAGATGAGC AATCTACTAT AAATATGAGA 252 GTACAAAATA CAGAAGGAAA CAACCTCAAC TAATTCACTC AGAATACATG 5189 |||||||||| |||| |||| |||||||||| ||||||||| ||||||||| GTACAAAATA CAGAGAGAAA CAACCTCAAC CAATTCACTC GGAATACATG 302 hqPGS_C06HBa0046G10.1-3+_SGN-E370622+ (4960 5189) ******************************************************************************** EST sequence 3 +strand 595 n (File: SGN-E271645+) 1 GATTAGTCAA TCCAAAATTT TTCCCTAAAA GGAAAACCTA TTTATGTTAA GAAATTATGG 61 TAAATAAAAT CCAACAAATC TCCTCCCCCT TGGCCTGTAT TTCTGACCAA AATAAATTTC 121 TCCACATTCT TCATTTAATC TTCAACAACT TGCTTCTCTT CTTCTTAATC TCCTTTGTAA 181 AATTTATGTC TCAACCATAG AAAACCTCTC TGAAACAATT TCTCCAACAA AATCTTCATT 241 ACTGTCAAAA AGATTGCGGC TAGAACCTAC CACCTGTCAA GATGAACCAC CACCCTCTTT 301 CTAACCTGGT CCAATAATCG ATTATCGAAC CACTGAACCT GACCTCTGTC ATTAAATGGC 361 TCTAATACCC ACTTGTTAGG ATCGAAATAA GCAGGTGTAA ATGCGGAAGC TAGCAAAGCA 421 AACTTCGAAA GACCACGAGT AAGAAGACAA CGAGAAATAT ACCAAAAGAC ACCAAAGATT 481 TAACGTGGTT CGGTCAATCG ACCTACGTTC ACAAAGGAGA TGAGCAATCC ACTATAAATA 541 TGAGAGTACA ATATACAGAG AGAAACAACA TCAACCAATT CATTCGGAAT ATATG Predicted gene structure (within gDNA segment 549 to 6195): Exon 1 4963 5189 ( 227 n); cDNA 369 595 ( 227 n); score: 0.896 MATCH C06HBa0046G10.1-3+ SGN-E271645+ 0.896 227 0.382 C PGS_C06HBa0046G10.1-3+_SGN-E271645+ (4963 5189) Alignment (genomic DNA sequence = upper lines): CCAATTGTTA GGACCGGAAA TAAGCAGGTG TAAATGCGGA AGCTAGCAAA ATAAATCTCG 5022 ||| |||||| ||| | |||| |||||||||| |||||||||| |||||||||| ||| ||| CCACTTGTTA GGATC-GAAA TAAGCAGGTG TAAATGCGGA AGCTAGCAAA GCAAACTTCG 427 AAAGATCACG AGTAAAAAAA CAACGAGAAA TATACCAAAA AACA-CAAAG ATTTAACGTG 5081 ||||| |||| ||||| || | |||||||||| |||||||||| ||| ||||| |||||||||| AAAGACCACG AGTAAGAAGA CAACGAGAAA TATACCAAAA GACACCAAAG ATTTAACGTG 487 GTTCGGTCAA TCGACCTACG TCCACAAAGG AGATGAGCAA TCCATTATAA ATATGAGAGT 5141 |||||||||| |||||||||| | |||||||| |||||||||| |||| ||||| |||||||||| GTTCGGTCAA TCGACCTACG TTCACAAAGG AGATGAGCAA TCCACTATAA ATATGAGAGT 547 ACAAAATACA GAAGGAAACA ACCTCAACTA ATTCACTCAG AATACATG 5189 |||| ||||| || |||||| || ||||| | ||||| || | |||| ||| ACAATATACA GAGAGAAACA ACATCAACCA ATTCATTCGG AATATATG 595 hqPGS_C06HBa0046G10.1-3+_SGN-E271645+ (4963 5189) ******************************************************************************** EST sequence 4 +strand 574 n (File: SGN-E206285+) 1 AACTAAGTAG AGCTATGTAT ATTTTGTCTG CAGAAGCAAG GGCTTAATTT ACAACTATAG 61 GTGGCGTACG TATAATGCAT GGACCGAAAA ATAAGCAGGT GTAAACGCGG AAGCTAGCAA 121 TGCTAACATC GAAAGACCAC GAGTAAGAAG ACAACGAGAA ATATACCAAA AGACACAAAG 181 ATTTAACGTG GTTCGGTCAA TCGACCTATG TCCACAAAGG AGATGAGCAA TCCACTATAA 241 ATATGAGAGT ACAAAATACA GAGGGAAACA ATCTCAACCA ATTCACTCAG AATACATGGG 301 AGGTTCACAC AAGTAATAAC GTATCAAGCT TGTGACCCAC AAATTCTCCC TCTAACCAAA 361 ACTCTCAAAG CCCTTAAGAC TACATTGTGA ATGTTAACTA AGTTAAAAGG AACATGCCTC 421 TATTTATAGA ATCCTAAACC TTTCCCTACA AGAAAAGGAT TAGTCAATCC AAAACCTTTT 481 CCTACAAGGA AAATCTATTT ATGGTAAGAA ATTTAGAGCA AATAAAACCC AACAAACATC 541 TATGATACGT GAACTTGCTT GGCCCTATAT GTTT Predicted gene structure (within gDNA segment 2942 to 7638): Exon 1 4974 5425 ( 452 n); cDNA 82 534 ( 453 n); score: 0.900 MATCH C06HBa0046G10.1-3+ SGN-E206285+ 0.900 452 0.787 C PGS_C06HBa0046G10.1-3+_SGN-E206285+ (4974 5425) Alignment (genomic DNA sequence = upper lines): GACCG-GAAA TAAGCAGGTG TAAATGCGGA AGCTAGCAAA ATAAATCTCG AAAGATCACG 5032 ||||| ||| |||||||||| |||| ||||| ||||||||| || ||| ||||| |||| GACCGAAAAA TAAGCAGGTG TAAACGCGGA AGCTAGCAAT GCTAACATCG AAAGACCACG 141 AGTAAAAAAA CAACGAGAAA TATACCAAAA AACACAAAGA TTTAACGTGG TTCGGTCAAT 5092 ||||| || | |||||||||| |||||||||| ||||||||| |||||||||| |||||||||| AGTAAGAAGA CAACGAGAAA TATACCAAAA GACACAAAGA TTTAACGTGG TTCGGTCAAT 201 CGACCTACGT CCACAAAGGA GATGAGCAAT CCATTATAAA TATGAGAGTA CAAAATACAG 5152 ||||||| || |||||||||| |||||||||| ||| |||||| |||||||||| |||||||||| CGACCTATGT CCACAAAGGA GATGAGCAAT CCACTATAAA TATGAGAGTA CAAAATACAG 261 AAGGAAACAA CCTCAACTAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTGATAACG 5212 | |||||||| |||||| || |||||||||| |||||||||| |||||||||| ||| |||||| AGGGAAACAA TCTCAACCAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTAATAACG 321 TATCAAGCTT GTGACCCATA AATTCTCCCC CTAACCAAAA CTCTCAAAGC CATTAAGACT 5272 |||||||||| |||||||| | ||||||||| |||||||||| |||||||||| | |||||||| TATCAAGCTT GTGACCCACA AATTCTCCCT CTAACCAAAA CTCTCAAAGC CCTTAAGACT 381 ACATTGTGAA TGCTGGTTAA GTTAGAAGGA ACATTCCTCT ATTTATAGAA TTCTAAACAT 5332 |||||||||| || | ||| |||| ||||| |||| ||||| |||||||||| | |||||| | ACATTGTGAA TGTTAACTAA GTTAAAAGGA ACATGCCTCT ATTTATAGAA TCCTAAACCT 441 TTTCCTACAA GAAAATGATT TAGTCAATCC AAAATTTTTT CCTAAAAAG- AAACCTATTT 5391 || ||||||| ||||| || | |||||||||| |||| |||| |||| || | ||| |||||| TTCCCTACAA GAAAAGGA-T TAGTCAATCC AAAACCTTTT CCTACAAGGA AAATCTATTT 500 ATGATAAGAA ATTTAGGGCA AATAAAACTC AACA 5425 ||| |||||| |||||| ||| |||||||| | |||| ATGGTAAGAA ATTTAGAGCA AATAAAACCC AACA 534 hqPGS_C06HBa0046G10.1-3+_SGN-E206285+ (4974 5425) ******************************************************************************** EST sequence 5 +strand 344 n (File: SGN-E206496+) 1 AACTAAGTAG AGCTATGTAT ATTTTGTCTG CAGAAGCAAT GGCTTAATTT ACAACTATAG 61 GTGGCGTACG TATAATGCAT GGACCGAAAA ATAAGCAGGT GTAAACGCGG AAGCTAGCAA 121 TGCTAACATC GAAAGACCAC GAGTAAGAAG ACAACGAGAA ATATACCAAA AGACACAAAG 181 ATTTAACGTG GTTCGGTCAA TCGACCTATG TCCACAAAGG AGATGAGCAA TCCACTATAA 241 ATATGAGAGT ACAAAATACA GAGGGAAACA ATCTCAACCA ATTCACTCAG AATACATGGG 301 AGGTTCACAC AAGTAATAAC GTATCAAGCT TGTGACCCAC AAAT Predicted gene structure (within gDNA segment 2942 to 5890): Exon 1 4974 5235 ( 262 n); cDNA 82 344 ( 263 n); score: 0.918 MATCH C06HBa0046G10.1-3+ SGN-E206496+ 0.918 262 0.762 C PGS_C06HBa0046G10.1-3+_SGN-E206496+ (4974 5235) Alignment (genomic DNA sequence = upper lines): GACCG-GAAA TAAGCAGGTG TAAATGCGGA AGCTAGCAAA ATAAATCTCG AAAGATCACG 5032 ||||| ||| |||||||||| |||| ||||| ||||||||| || ||| ||||| |||| GACCGAAAAA TAAGCAGGTG TAAACGCGGA AGCTAGCAAT GCTAACATCG AAAGACCACG 141 AGTAAAAAAA CAACGAGAAA TATACCAAAA AACACAAAGA TTTAACGTGG TTCGGTCAAT 5092 ||||| || | |||||||||| |||||||||| ||||||||| |||||||||| |||||||||| AGTAAGAAGA CAACGAGAAA TATACCAAAA GACACAAAGA TTTAACGTGG TTCGGTCAAT 201 CGACCTACGT CCACAAAGGA GATGAGCAAT CCATTATAAA TATGAGAGTA CAAAATACAG 5152 ||||||| || |||||||||| |||||||||| ||| |||||| |||||||||| |||||||||| CGACCTATGT CCACAAAGGA GATGAGCAAT CCACTATAAA TATGAGAGTA CAAAATACAG 261 AAGGAAACAA CCTCAACTAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTGATAACG 5212 | |||||||| |||||| || |||||||||| |||||||||| |||||||||| ||| |||||| AGGGAAACAA TCTCAACCAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTAATAACG 321 TATCAAGCTT GTGACCCATA AAT 5235 |||||||||| |||||||| | ||| TATCAAGCTT GTGACCCACA AAT 344 hqPGS_C06HBa0046G10.1-3+_SGN-E206496+ (4974 5235) ******************************************************************************** EST sequence 7 +strand 349 n (File: SGN-E207598+) 1 GAAAAGAAAC TAAGTAGAGC TATGTATATT TTGTCTGCAG AAGCAATGGC TTAATTTACA 61 ACTATAGGTG GCGTACGTAT AATGCATGGA CCGAAAAATA AGCAGGTGTA AACGCGGAAG 121 CTAGCAATGC TAACATCGAA AGACCACGAG TTAGAAGACA ACGAGAAATA TACCAAAAGA 181 CACAAAGATT TAACGTGGGT CGGTCAATCG ACCTATGTCC ACAAAGGAGA TGAGCAATCC 241 ACTATTAATA TGAGAGTACA AAATACAGAG GGAAACAATC TCAACCAATT CACTCAGAAT 301 ACATGGGAGG GTCACACAAG TAATAACGTA TCAAGCTTGT GACCCACAA Predicted gene structure (within gDNA segment 2872 to 5870): Exon 1 4974 5233 ( 260 n); cDNA 89 349 ( 261 n); score: 0.902 MATCH C06HBa0046G10.1-3+ SGN-E207598+ 0.902 260 0.745 C PGS_C06HBa0046G10.1-3+_SGN-E207598+ (4974 5233) Alignment (genomic DNA sequence = upper lines): GACCG-GAAA TAAGCAGGTG TAAATGCGGA AGCTAGCAAA ATAAATCTCG AAAGATCACG 5032 ||||| ||| |||||||||| |||| ||||| ||||||||| || ||| ||||| |||| GACCGAAAAA TAAGCAGGTG TAAACGCGGA AGCTAGCAAT GCTAACATCG AAAGACCACG 148 AGTAAAAAAA CAACGAGAAA TATACCAAAA AACACAAAGA TTTAACGTGG TTCGGTCAAT 5092 ||| | || | |||||||||| |||||||||| ||||||||| |||||||||| ||||||||| AGTTAGAAGA CAACGAGAAA TATACCAAAA GACACAAAGA TTTAACGTGG GTCGGTCAAT 208 CGACCTACGT CCACAAAGGA GATGAGCAAT CCATTATAAA TATGAGAGTA CAAAATACAG 5152 ||||||| || |||||||||| |||||||||| ||| ||| || |||||||||| |||||||||| CGACCTATGT CCACAAAGGA GATGAGCAAT CCACTATTAA TATGAGAGTA CAAAATACAG 268 AAGGAAACAA CCTCAACTAA TTCACTCAGA ATACATGGGA GGTTCACACA AGTGATAACG 5212 | |||||||| |||||| || |||||||||| |||||||||| || ||||||| ||| |||||| AGGGAAACAA TCTCAACCAA TTCACTCAGA ATACATGGGA GGGTCACACA AGTAATAACG 328 TATCAAGCTT GTGACCCATA A 5233 |||||||||| |||||||| | | TATCAAGCTT GTGACCCACA A 349 hqPGS_C06HBa0046G10.1-3+_SGN-E207598+ (4974 5233) ******************************************************************************** EST sequence 9 -strand 490 n (File: SGN-E205772-) 1 GGTGTAAACG CGGAAGCTAG CAATACAAAC CTCGAAAGAC CACGAGTAAG AAGACAACGA 61 GAAATATATC AAAAGACACA AAGATTTAAC GTGGTTCGGT CAATCAACCT ACGTCCACAA 121 AGGAGATGAG CAATCCACTA TAAATATAAG AGTACAAAAT ACAGAGAGAA TCAACCTCAA 181 CCAATTCACT CAGAATACAT GGGAGGTTCA CACAAGTGAT AACATATCAA GCTTGTGACC 241 CACAGATTCT CCCTCTAACC AAAACTCTCA AAGCCTGTAA GAGTACATTG TGAATGCTGA 301 TTAAGTTAAA AGGAATATTC ATCTATTTAT AGAGTCCTAA ACCTTTTCCT ACAAGAAAAG 361 GATTAGTCAA TTCAAAACCT TTTCCTAAAA GGAAAACCTA TTTATGGTAA GAAATCAGGG 421 CAAATAAAAT CCAACAAGAT ACTTAAAAGA TGAGCAAGAG TGGCTAATGG ATTTAAATTA 481 ACAAATTGTT Predicted gene structure (within gDNA segment 3704 to 7638): Exon 1 4989 5425 ( 437 n); cDNA 1 436 ( 436 n); score: 0.900 MATCH C06HBa0046G10.1-3+ SGN-E205772- 0.900 437 0.892 C PGS_C06HBa0046G10.1-3+_SGN-E205772- (4989 5425) Alignment (genomic DNA sequence = upper lines): GGTGTAAATG CGGAAGCTAG CAAAATAAAT CTCGAAAGAT CACGAGTAAA AAAACAACGA 5048 |||||||| | |||||||||| ||| | ||| ||||||||| ||||||||| || ||||||| GGTGTAAACG CGGAAGCTAG CAATACAAAC CTCGAAAGAC CACGAGTAAG AAGACAACGA 60 GAAATATACC AAAAAACACA AAGATTTAAC GTGGTTCGGT CAATCGACCT ACGTCCACAA 5108 |||||||| | |||| ||||| |||||||||| |||||||||| ||||| |||| |||||||||| GAAATATATC AAAAGACACA AAGATTTAAC GTGGTTCGGT CAATCAACCT ACGTCCACAA 120 AGGAGATGAG CAATCCATTA TAAATATGAG AGTACAAAAT ACAGAAGGAA ACAACCTCAA 5168 |||||||||| ||||||| || ||||||| || |||||||||| ||||| ||| ||||||||| AGGAGATGAG CAATCCACTA TAAATATAAG AGTACAAAAT ACAGAGAGAA TCAACCTCAA 180 CTAATTCACT CAGAATACAT GGGAGGTTCA CACAAGTGAT AACGTATCAA GCTTGTGACC 5228 | |||||||| |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| CCAATTCACT CAGAATACAT GGGAGGTTCA CACAAGTGAT AACATATCAA GCTTGTGACC 240 CATAAATTCT CCCCCTAACC AAAACTCTCA AAGCCATTAA GACTACATTG TGAATGCTGG 5288 || | ||||| ||| |||||| |||||||||| ||||| ||| || ||||||| ||||||||| CACAGATTCT CCCTCTAACC AAAACTCTCA AAGCCTGTAA GAGTACATTG TGAATGCTGA 300 TTAAGTTAGA AGGAACATTC CTCTATTTAT AGAATTCTAA ACATTTTCCT ACAAGAAAAT 5348 |||||||| | ||||| |||| ||||||||| ||| | |||| || ||||||| ||||||||| TTAAGTTAAA AGGAATATTC ATCTATTTAT AGAGTCCTAA ACCTTTTCCT ACAAGAAAAG 360 GATTTAGTCA ATCCAAAATT TTTTCCTAAA AAG-AAACCT ATTTATGATA AGAAATTTAG 5407 || ||||||| || ||||| |||||||||| | | |||||| ||||||| || ||||| | || GA-TTAGTCA ATTCAAAACC TTTTCCTAAA AGGAAAACCT ATTTATGGTA AGAAA-TCAG 418 GGCAAATAAA ACTCAACA 5425 |||||||||| | ||||| GGCAAATAAA ATCCAACA 436 hqPGS_C06HBa0046G10.1-3+_SGN-E205772- (4989 5425) ******************************************************************************** EST sequence 10 -strand 456 n (File: SGN-E205773-) 1 AAAGACCACG AGTAAGAAGC CATCGAGAAA TATATCAAAA GACACAAAGA TTTAACGTGG 61 TTCGGTCAAT CAACCTACGT CCACAAAGTA GATGAGCAAT CCACTATAAA TATAAGAGTA 121 CAAAATACAG AGAGAAACAG CTTCAACCAA TTCACTCAGA ATACATGGTA GGTTCACACA 181 AGTGATAACA TATCAAGCTT GTGACCCACA GATTCTCCCT CTAACCAAAA CTCTCAAAGC 241 CTGTAAGACT ACATTGTGAA TGCTGATTAA GTTAAAAGGA ATATTCATCT ATTTATAGAG 301 TCCTAAACCT TTTCCTACAA GAAAAGGATT AGTCAATTCA AAACCTTTTC CTAAAAGGAA 361 AACCTATTTA TGGTAAGAAA TCAGGGCAAA TAAAATCCAA CAAGATACTT AAAAGATGAG 421 CAAGAGTGGC TAATGGATTT AAATTAACTA ATTGTT Predicted gene structure (within gDNA segment 4044 to 7638): Exon 1 5023 5425 ( 403 n); cDNA 1 402 ( 402 n); score: 0.892 MATCH C06HBa0046G10.1-3+ SGN-E205773- 0.892 403 0.884 C PGS_C06HBa0046G10.1-3+_SGN-E205773- (5023 5425) Alignment (genomic DNA sequence = upper lines): AAAGATCACG AGTAAAAAAA CAACGAGAAA TATACCAAAA AACACAAAGA TTTAACGTGG 5082 ||||| |||| ||||| || || ||||||| |||| ||||| ||||||||| |||||||||| AAAGACCACG AGTAAGAAGC CATCGAGAAA TATATCAAAA GACACAAAGA TTTAACGTGG 60 TTCGGTCAAT CGACCTACGT CCACAAAGGA GATGAGCAAT CCATTATAAA TATGAGAGTA 5142 |||||||||| | |||||||| |||||||| | |||||||||| ||| |||||| ||| |||||| TTCGGTCAAT CAACCTACGT CCACAAAGTA GATGAGCAAT CCACTATAAA TATAAGAGTA 120 CAAAATACAG AAGGAAACAA CCTCAACTAA TTCACTCAGA ATACATGGGA GGTTCACACA 5202 |||||||||| | |||||| | ||||| || |||||||||| |||||||| | |||||||||| CAAAATACAG AGAGAAACAG CTTCAACCAA TTCACTCAGA ATACATGGTA GGTTCACACA 180 AGTGATAACG TATCAAGCTT GTGACCCATA AATTCTCCCC CTAACCAAAA CTCTCAAAGC 5262 ||||||||| |||||||||| |||||||| | |||||||| |||||||||| |||||||||| AGTGATAACA TATCAAGCTT GTGACCCACA GATTCTCCCT CTAACCAAAA CTCTCAAAGC 240 CATTAAGACT ACATTGTGAA TGCTGGTTAA GTTAGAAGGA ACATTCCTCT ATTTATAGAA 5322 | ||||||| |||||||||| ||||| |||| |||| ||||| | |||| ||| ||||||||| CTGTAAGACT ACATTGTGAA TGCTGATTAA GTTAAAAGGA ATATTCATCT ATTTATAGAG 300 TTCTAAACAT TTTCCTACAA GAAAATGATT TAGTCAATCC AAAATTTTTT CCTAAAAAG- 5381 | |||||| | |||||||||| ||||| || | |||||||| | |||| |||| ||||||| | TCCTAAACCT TTTCCTACAA GAAAAGGA-T TAGTCAATTC AAAACCTTTT CCTAAAAGGA 359 AAACCTATTT ATGATAAGAA ATTTAGGGCA AATAAAACTC AACA 5425 |||||||||| ||| |||||| | | |||||| ||||||| | |||| AAACCTATTT ATGGTAAGAA A-TCAGGGCA AATAAAATCC AACA 402 hqPGS_C06HBa0046G10.1-3+_SGN-E205773- (5023 5425) ******************************************************************************** EST sequence 6 +strand 535 n (File: SGN-E243194+) 1 AAAAGACATA AAGATTTAAC GTGGTTCGGT CAATCGACCT ACGTCCACAA AGGAGATGAG 61 CAATCCACTA TAAATATGAG AGTACAAAAT ACAGAGGGAA ACAACCTCAA CCAATTCACT 121 CAGAATACAT GGGAGGTTCA CACAAGTGAT AACGTATCAA GCTTGTGACC CACAAATTCT 181 CCCTCTAACC AAAACTCTCA AAGCACTTAA GACTACATTT TGAATGCTGA TTAAGTTAGA 241 AGAAAAATGC CTCTATTTAT GGAGTCCTAA ACCTTTTCCT ACAAGAAAAG GATTAGTCAA 301 TCCAAAACCT TTTCCTACAA GAAAAGGATT AGTCAATCCA AAACCTTTTC CTACAAGGAA 361 AACCTATTTA TGGTAAGAAA TTTAGGGCAA ATAAAACCCA ACAAGTCTCC CCCTTGGCCT 421 GAATTTCTGA CAAATAAACT TGTCCACCTT CTTCACTTAA TCTTCAACAA CTTGCTTCTC 481 CTCTCCATAA TCTCCTTTGC AAAATTTATG TCTCAACACA AAGAATCTCT CTGAA Predicted gene structure (within gDNA segment 4378 to 7638): Exon 1 5059 5390 ( 332 n); cDNA 1 330 ( 330 n); score: 0.919 MATCH C06HBa0046G10.1-3+ SGN-E243194+ 0.919 332 0.621 C PGS_C06HBa0046G10.1-3+_SGN-E243194+ (5059 5390) Alignment (genomic DNA sequence = upper lines): AAAAAACACA AAGATTTAAC GTGGTTCGGT CAATCGACCT ACGTCCACAA AGGAGATGAG 5118 |||| ||| | |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAAGACATA AAGATTTAAC GTGGTTCGGT CAATCGACCT ACGTCCACAA AGGAGATGAG 60 CAATCCATTA TAAATATGAG AGTACAAAAT ACAGAAGGAA ACAACCTCAA CTAATTCACT 5178 ||||||| || |||||||||| |||||||||| ||||| |||| |||||||||| | |||||||| CAATCCACTA TAAATATGAG AGTACAAAAT ACAGAGGGAA ACAACCTCAA CCAATTCACT 120 CAGAATACAT GGGAGGTTCA CACAAGTGAT AACGTATCAA GCTTGTGACC CATAAATTCT 5238 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || ||||||| CAGAATACAT GGGAGGTTCA CACAAGTGAT AACGTATCAA GCTTGTGACC CACAAATTCT 180 CCCCCTAACC AAAACTCTCA AAGCCATTAA GACTACATTG TGAATGCTGG TTAAGTTAGA 5298 ||| |||||| |||||||||| |||| |||| ||||||||| ||||||||| |||||||||| CCCTCTAACC AAAACTCTCA AAGCACTTAA GACTACATTT TGAATGCTGA TTAAGTTAGA 240 AGGAACATTC CTCTATTTAT AGAATTCTAA ACATTTTCCT ACAAGAAAAT GATTTAGTCA 5358 || || || | |||||||||| || | |||| || ||||||| ||||||||| || ||||||| AGAAAAATGC CTCTATTTAT GGAGTCCTAA ACCTTTTCCT ACAAGAAAAG GA-TTAGTCA 299 ATCCAAAATT TTTTCCTAAA AAGAAACCTA TT 5390 |||||||| ||||||| | |||||| | || ATCCAAAACC TTTTCCT-AC AAGAAAAGGA TT 330 hqPGS_C06HBa0046G10.1-3+_SGN-E243194+ (5059 5390) Total number of EST alignments reported: 11 ________________________________________________________________________________ Predicted gene locations (1) in segment 1 to 7638: PGL 1 (+ strand): 3494 5438 AGS-1 (3494 3535,4960 5425) SCR (e 0.762 d 0.000 a 0.431,e 0.900) Exon 1 3494 3535 ( 42 n); score: 0.762 Intron 1 3536 4959 (1424 n); Pd: 0.000 Pa: 0.431 Exon 2 4960 5425 ( 466 n); score: 0.900 PGS (3494 3535,4960 5218) SGN-E389613+ PGS (4957 5425) SGN-E211090- PGS (4960 5189) SGN-E370622+ PGS (4963 5189) SGN-E271645+ PGS (4974 5425) SGN-E206285+ PGS (4974 5235) SGN-E206496+ PGS (4974 5233) SGN-E207598+ PGS (4989 5425) SGN-E205772- PGS (5023 5425) SGN-E205773- PGS (5059 5390) SGN-E243194+ 3-phase translation of AGS-1 (+strand): . . . . . : . 3494 AACTCCATAGCCCTTAAAGTAATTTAGAGTCATAAGGAACCT : TGACCAATTGTTAGGACC N S I A L K V I - S H K E P : - P I V R T T P - P L K - F R V I R N L : D Q L L G P L H S P - S N L E S - G T : L T N C - D . . . . . . 4978 GGAAATAAGCAGGTGTAAATGCGGAAGCTAGCAAAATAAATCTCGAAAGATCACGAGTAA G N K Q V - M R K L A K - I S K D H E - E I S R C K C G S - Q N K S R K I T S K R K - A G V N A E A S K I N L E R S R V . . . . . . 5038 AAAAACAACGAGAAATATACCAAAAAACACAAAGATTTAACGTGGTTCGGTCAATCGACC K N N E K Y T K K H K D L T W F G Q S T K T T R N I P K N T K I - R G S V N R P K K Q R E I Y Q K T Q R F N V V R S I D . . . . . . 5098 TACGTCCACAAAGGAGATGAGCAATCCATTATAAATATGAGAGTACAAAATACAGAAGGA Y V H K G D E Q S I I N M R V Q N T E G T S T K E M S N P L - I - E Y K I Q K E L R P Q R R - A I H Y K Y E S T K Y R R . . . . . . 5158 AACAACCTCAACTAATTCACTCAGAATACATGGGAGGTTCACACAAGTGATAACGTATCA N N L N - F T Q N T W E V H T S D N V S T T S T N S L R I H G R F T Q V I T Y Q K Q P Q L I H S E Y M G G S H K - - R I . . . . . . 5218 AGCTTGTGACCCATAAATTCTCCCCCTAACCAAAACTCTCAAAGCCATTAAGACTACATT S L - P I N S P P N Q N S Q S H - D Y I A C D P - I L P L T K T L K A I K T T L K L V T H K F S P - P K L S K P L R L H . . . . . . 5278 GTGAATGCTGGTTAAGTTAGAAGGAACATTCCTCTATTTATAGAATTCTAAACATTTTCC V N A G - V R R N I P L F I E F - T F S - M L V K L E G T F L Y L - N S K H F P C E C W L S - K E H S S I Y R I L N I F . . . . . . 5338 TACAAGAAAATGATTTAGTCAATCCAAAATTTTTTCCTAAAAAGAAACCTATTTATGATA Y K K M I - S I Q N F F L K R N L F M I T R K - F S Q S K I F S - K E T Y L - - L Q E N D L V N P K F F P K K K P I Y D . . . 5398 AGAAATTTAGGGCAAATAAAACTCAACA R N L G Q I K L N E I - G K - N S T K K F R A N K T Q Maximal non-overlapping open reading frames (>= 64 codons): none AGS-2 (4975 5191,5328 5438) SCR (e 0.894 d 0.000 a 0.000,e 0.806) Exon 1 4975 5191 ( 217 n); score: 0.894 Intron 1 5192 5327 ( 136 n); Pd: 0.000 Pa: 0.000 Exon 2 5328 5438 ( 111 n); score: 0.806 PGS (4975 5191,5328 5438) SGN-E543331- 3-phase translation of AGS-2 (+strand): . . . . . . 4975 ACCGGAAATAAGCAGGTGTAAATGCGGAAGCTAGCAAAATAAATCTCGAAAGATCACGAG T G N K Q V - M R K L A K - I S K D H E P E I S R C K C G S - Q N K S R K I T S R K - A G V N A E A S K I N L E R S R . . . . . . 5035 TAAAAAAACAACGAGAAATATACCAAAAAACACAAAGATTTAACGTGGTTCGGTCAATCG - K N N E K Y T K K H K D L T W F G Q S K K T T R N I P K N T K I - R G S V N R V K K Q R E I Y Q K T Q R F N V V R S I . . . . . . 5095 ACCTACGTCCACAAAGGAGATGAGCAATCCATTATAAATATGAGAGTACAAAATACAGAA T Y V H K G D E Q S I I N M R V Q N T E P T S T K E M S N P L - I - E Y K I Q K D L R P Q R R - A I H Y K Y E S T K Y R . . . . : . . 5155 GGAAACAACCTCAACTAATTCACTCAGAATACATGGG : AACATTTTCCTACAAGAAAATGA G N N L N - F T Q N T W : E H F P T R K - E T T S T N S L R I H G : N I F L Q E N D R K Q P Q L I H S E Y M G : T F S Y K K M . . . . . . 5351 TTTAGTCAATCCAAAATTTTTTCCTAAAAAGAAACCTATTTATGATAAGAAATTTAGGGC F S Q S K I F S - K E T Y L - - E I - G L V N P K F F P K K K P I Y D K K F R A I - S I Q N F F L K R N L F M I R N L G . . . 5411 AAATAAAACTCAACACCAATAAGTGAAT K - N S T P I S E N K T Q H Q - V N Q I K L N T N K - Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Mon Aug 28 21:41:11 2006 ________________________________________________________________________________ Sequence 4: C06HBa0046G10.1-4, from 1 to 25558, both strands analyzed. ... started at: Mon Aug 28 21:41:11 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 7 ... matches indexed, elapsed seconds = 7 HitsTableSize = 18 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 11 ******************************************************************************** EST sequence 12 +strand 631 n (File: SGN-E542618+) 1 AAAAAATATG AAACAAAACA CCTTCCATAA ACAAAGTGAC GACGACGTCG GATTAAGTCC 61 CTCGCAGAAA CCCCAAATCG AAATTCTCGG TCCTATAAAC CCCAGGTGTT TTCTTCTTCT 121 TCTTCCCAAT TCTCAAAATC TTACCATTTC TCGCCATGGA TGAAGAAGAG ATTGAGAAAG 181 CAGTCGTAGC GTATTTGAAG AAGAAGGGTT TCAAGCAAAC GGAGATTGCT TTTCAGGAAG 241 AACAGCAAGT GAATAAAAAC AGTTCTTCTT CAATCCATAA TAACAACAAC TCAGCAAATG 301 TCTCAGATTG ACCCCGACTT AACCAAAAAA ATCCTTTCTT TCTCTGAATT TGAGACCAGT 361 CCCCAACGAT ACCAAGAAGA ATATAGCATA CTTCAGTCAT GGGCCTATAG TTCACTGGAT 421 CTGTATAAGC ATGAGTTGCT TCGAGTATTA TATCCTGTAT TTATTCATTG CTTCATGGAA 481 CTTGTCGCTA GAGGTTATAT TCAAGAAGCA CGAGCCTTTT TCAATAGTTA CCGTGAAGAC 541 CATGAGATGA CGCATCTGCG TGATCTTCAG AAGTTAGAAG GCGTCCTCTC ACCTTCCCAT 601 CTAGAGGAAA CGGAATTTGC TCATTCTATA C Predicted gene structure (within gDNA segment 2443 to 7831): Exon 1 3133 3477 ( 345 n); cDNA 1 347 ( 347 n); score: 0.974 Intron 1 3478 4650 (1173 n); Pd: 0.998 (s: 0.95), Pa: 0.999 (s: 0.94) Exon 2 4651 4732 ( 82 n); cDNA 348 429 ( 82 n); score: 0.963 Intron 2 4733 6726 (1994 n); Pd: 0.815 (s: 0.96), Pa: 0.328 (s: 0.94) Exon 3 6727 6805 ( 79 n); cDNA 430 508 ( 79 n); score: 0.962 Intron 3 6806 6882 ( 77 n); Pd: 0.900 (s: 0.98), Pa: 0.975 (s: 1.00) Exon 4 6883 6980 ( 98 n); cDNA 509 606 ( 98 n); score: 1.000 Intron 4 6981 7065 ( 85 n); Pd: 0.979 (s: 1.00), Pa: 0.985 (s: 0) Exon 5 7066 7090 ( 25 n); cDNA 607 631 ( 25 n); score: 0.960 MATCH C06HBa0046G10.1-4+ SGN-E542618+ 0.975 629 0.997 C PGS_C06HBa0046G10.1-4+_SGN-E542618+ (3133 3477,4651 4732,6727 6805,6883 6980,7066 7090) Alignment (genomic DNA sequence = upper lines): AAAAAATATA AAACAAAACA CCTTCCATAA ACAAAGTGAC GACGACGTCG TATTAAGTCC 3192 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AAAAAATATG AAACAAAACA CCTTCCATAA ACAAAGTGAC GACGACGTCG GATTAAGTCC 60 CTC-CAGAAA CCCCAAATCA AAATTCTCGG TCCTATAAAC CCCAGGTGTT TTCTTCTTCT 3251 ||| |||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCGCAGAAA CCCCAAATCG AAATTCTCGG TCCTATAAAC CCCAGGTGTT TTCTTCTTCT 120 TCTTCCCAAT TCTCAAAATC TTACCATTTC TCGCCATGGA TGAAGAAGAG ATTGAGAAAG 3311 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTCCCAAT TCTCAAAATC TTACCATTTC TCGCCATGGA TGAAGAAGAG ATTGAGAAAG 180 CAGTCGTAGC GTATTTGAAG AAGAAGGGTT TCAAGCAAAC GGAGATTGCT TTTCAAGAAG 3371 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| |||| CAGTCGTAGC GTATTTGAAG AAGAAGGGTT TCAAGCAAAC GGAGATTGCT TTTCAGGAAG 240 AACAGCAAGT GAATAAAAAC AGTTCTTCTT CAATCCATAA TAACAACAAC TCAGCAAAT- 3430 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AACAGCAAGT GAATAAAAAC AGTTCTTCTT CAATCCATAA TAACAACAAC TCAGCAAATG 300 TCTCAGATTG ACCCCGACTT AACCAAAAAA ATCCTTTCTT TCTCTGAGTA AGTTTCTTTT 3490 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TCTCAGATTG ACCCCGACTT AACCAAAAAA ATCCTTTCTT TCTCTGA... .......... 347 TTCCACTTAA TTTGATGTAA AAATTACTGT TTGGCTAAGA GGATTAAGTT CTAAAATCAA 3550 .......... .......... .......... .......... .......... .......... 347 TTTACCTTGA AAGATAGTTT AATTTCAATG AAACACGTTT AAGTAGAGAA GGATTGAGTA 3610 .......... .......... .......... .......... .......... .......... 347 CAGAAGATCT GAAAACTAGT TTAGGGTTGA GAGGTGACTT GGTTGTTGGA AGAAAAGCAG 3670 .......... .......... .......... .......... .......... .......... 347 GAGCAATATA ATTGAAGCAG TTTTTCTGCA CCCTCCAGTA GCATTTCTGC AAATGCCCAA 3730 .......... .......... .......... .......... .......... .......... 347 ATTGACCCTG AACCGTCAGA CAAAAATAAT CTGTTCTCTG TAGATAACAT AAATTTAATT 3790 .......... .......... .......... .......... .......... .......... 347 ACTGGACCCA TTCTAAAGAG GGAGAAATCA GCACTACTTG GTGAAATAGG TTCTTTTTGA 3850 .......... .......... .......... .......... .......... .......... 347 ATCAATTGTC CTCTTCTCCT GTATTTTAAA TGCAGAGTGC TTAGCCATGA GGTTGCGGTT 3910 .......... .......... .......... .......... .......... .......... 347 CTAAATAGCA TTGACAATGC CCAGAACCAC TTTTGAGGTA CTAGCACACT GTCTAGGAAT 3970 .......... .......... .......... .......... .......... .......... 347 TGGAAAGAGA GGATCAAAGG AAATTTGGCG GAAGAATATC CCTGTTTGTA TCTGGTGGAC 4030 .......... .......... .......... .......... .......... .......... 347 GTTATGAAAA AAAAAGGAAT GAGAGATATT ATGAAGGGAA GGGTAGCAGC ACACAGAAGA 4090 .......... .......... .......... .......... .......... .......... 347 TCAAGATGAG ATCTCTTAGT ATTATATTTT TTTGGTGTAA GCAAGATTTA GTGGGGACAA 4150 .......... .......... .......... .......... .......... .......... 347 TAGATAGCTT AGTGGAGATT ATAGGCCAAC TGTAAATTTT TGAAAGTTTG TTTTTGGATT 4210 .......... .......... .......... .......... .......... .......... 347 TTGAGTATGA TGGCATCCCC TGGGGTTTGT AAATTCCTCC TCATCAGTCT GATGAGTTCT 4270 .......... .......... .......... .......... .......... .......... 347 TTTGTGCGGA TTTAAGTTAC CTATTTCAAA ATAAATAAAT AAATAGAGGG GATGTAGAGG 4330 .......... .......... .......... .......... .......... .......... 347 AGGATTTATG AGCTGACCCA AAGTCTCAAA CTGTTTAGGA TTGAGGCTTC ATTAGTTGTT 4390 .......... .......... .......... .......... .......... .......... 347 TCAAGAAGGG AAGCAACAAT AATTGTGTGT TTGGTATGGA GGAAGCTTTG GTGTTATTTT 4450 .......... .......... .......... .......... .......... .......... 347 ACATTCTATT AGTGACATTA CACTTTGGAG CTTCCTACTT TTAGAATATT ACAGATATAG 4510 .......... .......... .......... .......... .......... .......... 347 ATGGATAGAT GTTATGGCTC CGTTTTCACA CCGCCTTACA CTAGCTGGAA GTCTTGACAG 4570 .......... .......... .......... .......... .......... .......... 347 CTGTATAACG CATACTTTCT GGTTATTTTC TTAGCATGCA TCTTTGATAT CATTCTGAAT 4630 .......... .......... .......... .......... .......... .......... 347 CTAAATTTTT ATGTTTTCAG ATTTGAAACC AGTCCCCAAC GATACCAAGA AGAATATAGC 4690 |||||| ||| |||||||||| |||||||||| |||||||||| .......... .......... ATTTGAGACC AGTCCCCAAC GATACCAAGA AGAATATAGC 387 AAACTTCGGT CATGGGCCTA TAGTTCACTG GATCTGTATA AGGTTGGTCC CCTGACTTGT 4750 | ||||| || |||||||||| |||||||||| |||||||||| || ATACTTCAGT CATGGGCCTA TAGTTCACTG GATCTGTATA AG........ .......... 429 GCTAACAGTA ACATGAGAAG TAGTTTATGC AGTATCATGA TGCATAGCTC CTCATGACAT 4810 .......... .......... .......... .......... .......... .......... 429 GTAATGCACT AATAATAGGT CTGCCACCAG TAACACCATA GGAAATAAAC ATAAATGGAA 4870 .......... .......... .......... .......... .......... .......... 429 AGGAAAGGCT TTCATTTTCT CTTTTATTTA GCCCTTTTTA ATCCTTAACA TAATGTATTA 4930 .......... .......... .......... .......... .......... .......... 429 GTGATGTGCA CCTTGACCTA TTAATACTTC TGCTGCTGTC TTTCTGGCAG TGACAAGGTC 4990 .......... .......... .......... .......... .......... .......... 429 TTCAAAATTG AGTTCTCATA TTTGTGATTC TCAACTCTAG TTCATTGACT TATGCTATAA 5050 .......... .......... .......... .......... .......... .......... 429 ATGTAGCACC AGTGATGGAT TTAGTACTGT CTTCTACCAT TGCGATCATA ATAGCGAGCC 5110 .......... .......... .......... .......... .......... .......... 429 AAAAATAGCT CTTCATAACA TAAGCATGAC AAGCGTGCCG TCTTCATACA AGTTAGGTTG 5170 .......... .......... .......... .......... .......... .......... 429 TGTGATTATA CAAGCCAGAT GAAAAAGTGA TGAAAATGGT TCCATATGTT TGGTAGGTTC 5230 .......... .......... .......... .......... .......... .......... 429 AAGTTGGTCC CTTAATACTC TTGAACAATT TTGGTCCTTT AAGTTCACCA AAAGCGAAAA 5290 .......... .......... .......... .......... .......... .......... 429 CACCCTTTGG GGGGGGGGGC AGTGGTTTGG GCTTGGGGCT TCCATTTTGG AGGTTTCAAG 5350 .......... .......... .......... .......... .......... .......... 429 TTCGAAACCC CTTGCTAGCA AAAGCAAGGG TCCAGTTTAG AAAATCAAGA GATAATTTAC 5410 .......... .......... .......... .......... .......... .......... 429 CTTTTGTGTC CTTTGTTATT AAATACTATT TATCATCTAA CACATTTTCC AGAGCATTAA 5470 .......... .......... .......... .......... .......... .......... 429 ATTTAATAAA GTCAAAGGAT AAAATAGTAA TGTTACCGCT ATTATTTATT GTTTCTTAAG 5530 .......... .......... .......... .......... .......... .......... 429 GGTGTGCTAA GTCAATAGTA GACAACTATT GTTGGACCAA GGGAGTAACA TCTATAGTTT 5590 .......... .......... .......... .......... .......... .......... 429 CAACGTTTGC CAGCTTGCTA ATTTTGGTTT ATTTTTGGAG GATGGTGCAG CTTTTGAAGT 5650 .......... .......... .......... .......... .......... .......... 429 GCAATTTTGC CATCTTTTAA TATCTATTGT GTGGTATATG GTTCAATTTT TGGTGTTGCA 5710 .......... .......... .......... .......... .......... .......... 429 ATTTTCAAGA TAGGACAGGA CTTTTTGGTG TTTTTGGTTG GATAGTTATT TTGACCATTC 5770 .......... .......... .......... .......... .......... .......... 429 CGATGAAGTC TACCCATTCA AGTTCTGTTT AAAAAAAAAA AGGGTCCAAC CACTTGAGTT 5830 .......... .......... .......... .......... .......... .......... 429 CTGTTTCAAA AAAAAAAGTC TTAACCATCC GAGTTTGTTA AATATTTGTC AGAGACCAAA 5890 .......... .......... .......... .......... .......... .......... 429 TGCTCACTTT TGGCAAACTT AGAAAACCCA AATTGTTGGG GGGTATATTG AAGGACCACC 5950 .......... .......... .......... .......... .......... .......... 429 TTGAACCTAT ACTCAAACAT AAGGAGCCAT TTTTTCTCTC TCTCCAAGTT AGACACTTCC 6010 .......... .......... .......... .......... .......... .......... 429 TTTTGACTTA CTCTTTAATC TTCAAGATTT TGTGGTTTCC TGTAATAGCT GAAGCTACCT 6070 .......... .......... .......... .......... .......... .......... 429 TCCATTTATT TTTGTCCATT TCTGTCTACC TTGTCTCAGT CCATTCCAGA TTGCCTCTCT 6130 .......... .......... .......... .......... .......... .......... 429 CTTTCTATTG GAATTGAAGG ATGTGCTGTG TGCATCTGGA TTTTCTCCAA TTCCAACATA 6190 .......... .......... .......... .......... .......... .......... 429 ATCTGATATT GATTGAAAAG AGGGAAGTTA AGCTAGCTAA CGAAGGGGTT GGGATCCGCC 6250 .......... .......... .......... .......... .......... .......... 429 TCGCCCTTAA AGATCCGCCC CTCGTTTCAC TCTGAAAATT TATTATCAGC TCCTTCTGTT 6310 .......... .......... .......... .......... .......... .......... 429 ATTTAGCCAT CTCTCTCCAT AAAATTCTTT CACGTACATG AAAGTTATTT CATATCCTAG 6370 .......... .......... .......... .......... .......... .......... 429 AGAGTGCGGT TGTATTGTCT CTCTAAAGAA TCGTAGGATC CATTGGAGAT AAAATGAAAC 6430 .......... .......... .......... .......... .......... .......... 429 AATTTTAAAT GCCGAGTGTT CTGTTTCCTT TTTTTCCTTT GGTCGTTTCT CTTTCTGCAG 6490 .......... .......... .......... .......... .......... .......... 429 TGCTGTTACT GCTTAGAATA CCTTTCGGTT CGCAGAAATG ATGTGCAAAC TTTGTTGTTT 6550 .......... .......... .......... .......... .......... .......... 429 CCAACAGTTT GGCCATTGTA TATATTGGGG TTGTTAAGTC TTTCTCTTTT ATAGTATGCG 6610 .......... .......... .......... .......... .......... .......... 429 TGACTCATTT AAAATCATCT TATATTATGT TGATTATTGT TATCCTCTTT TATTTGTTAG 6670 .......... .......... .......... .......... .......... .......... 429 ATGAGATTAT TGTTATTATT GATTCATCTG AACATATATA TATTTCAATC TTGCAGCATG 6730 |||| .......... .......... .......... .......... .......... ......CATG 433 AGTTGCTTCG TGTTTTATAT CCTGTATTTA TTCATTGTTT CATGGAACTT GTCGCTAGAG 6790 |||||||||| || |||||| |||||||||| ||||||| || |||||||||| |||||||||| AGTTGCTTCG AGTATTATAT CCTGTATTTA TTCATTGCTT CATGGAACTT GTCGCTAGAG 493 GTTATATTCA AGAAGGTATA CTTTGTCAGT TATGTTTTGG TTGTATTTTG ATATTTGTGA 6850 |||||||||| ||||| GTTATATTCA AGAAG..... .......... .......... .......... .......... 508 GAGAATTTTT AACACAATTC TTTTATTTCC AGCACGAGCC TTTTTCAATA GTTACCGTGA 6910 |||||||| |||||||||| |||||||||| .......... .......... .......... ..CACGAGCC TTTTTCAATA GTTACCGTGA 536 AGACCATGAG ATGACGCATC TGCGTGATCT TCAGAAGTTA GAAGGCGTCC TCTCACCTTC 6970 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGACCATGAG ATGACGCATC TGCGTGATCT TCAGAAGTTA GAAGGCGTCC TCTCACCTTC 596 CCATCTAGAG GTACATCAAT ATCCTAGCAA CTGTGTGGAT GACCTTTTCC ATGCTTAAGT 7030 |||||||||| CCATCTAGAG .......... .......... .......... .......... .......... 606 GTGTCATAGA TCACTAAACT TATTCCGAAA TGCAGGAAAT GGAATTTGCT CATTCTATAC 7090 |||| |||||||||| |||||||||| .......... .......... .......... .....GAAAC GGAATTTGCT CATTCTATAC 631 hqPGS_C06HBa0046G10.1-4+_SGN-E542618+ (3133 3477,4651 4732,6727 6805,6883 6980,7066 7090) ******************************************************************************** EST sequence 13 +strand 561 n (File: SGN-E542658+) 1 AAAAAATATA AAACAAAACA CCTTCCATAA ACAAAGTGAC GACTACGTCT TATTAAGTCC 61 CTCCAGAAAC CCCAAATCAA AATTCTCGGT CCTATAAACC CCAGGTGTTT TCTTCTTCTT 121 CTTCCCAATT CTCAAAATCT TACCATTTCT CGCCATGGAT GAAGAAGAGA TTGAGAAAGC 181 AGTCGTAGCG TATTTGAAGA AGAAGGGTTT CAAGCAAACG GAGATTGCTT TTCAAGAAGA 241 ACAGCAAGTG AATAAAAACA GTTCTTCTTC AATCCATAAT AACAACAACT CAGCAAATTC 301 TCAGATTGAC CCCGACTTAA CCAAAAAAAT CCTTTCTTTC TCTGAATTTG AAACCAGTCC 361 CCTACGATAC CAAGAAGAAT ATAGCATACT TCAGTCATGG GCCTATAGTT CACTGGATCT 421 GTATAAGCAT GAGTTGCTTC GAGTATTATA TCCTGTATTT ATTCATTGCT TCATGGAACT 481 TGTCGCTAGA GGTTATATTC TAGAAGCACG AGCCTTTTTC AATAGTTACC GTGAAGACCA 541 TGAGATGACG CATCTGCGTG G Predicted gene structure (within gDNA segment 2533 to 7556): Exon 1 3133 3477 ( 345 n); cDNA 1 345 ( 345 n); score: 0.994 Intron 1 3478 4650 (1173 n); Pd: 0.998 (s: 1.00), Pa: 0.999 (s: 0.94) Exon 2 4651 4732 ( 82 n); cDNA 346 427 ( 82 n); score: 0.963 Intron 2 4733 6726 (1994 n); Pd: 0.815 (s: 0.96), Pa: 0.328 (s: 0.94) Exon 3 6727 6805 ( 79 n); cDNA 428 506 ( 79 n); score: 0.949 Intron 3 6806 6882 ( 77 n); Pd: 0.900 (s: 0.96), Pa: 0.975 (s: 1.00) Exon 4 6883 6936 ( 54 n); cDNA 507 560 ( 54 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E542658+ 0.984 560 0.998 C PGS_C06HBa0046G10.1-4+_SGN-E542658+ (3133 3477,4651 4732,6727 6805,6883 6936) Alignment (genomic DNA sequence = upper lines): AAAAAATATA AAACAAAACA CCTTCCATAA ACAAAGTGAC GACGACGTCG TATTAAGTCC 3192 |||||||||| |||||||||| |||||||||| |||||||||| ||| ||||| |||||||||| AAAAAATATA AAACAAAACA CCTTCCATAA ACAAAGTGAC GACTACGTCT TATTAAGTCC 60 CTCCAGAAAC CCCAAATCAA AATTCTCGGT CCTATAAACC CCAGGTGTTT TCTTCTTCTT 3252 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCAGAAAC CCCAAATCAA AATTCTCGGT CCTATAAACC CCAGGTGTTT TCTTCTTCTT 120 CTTCCCAATT CTCAAAATCT TACCATTTCT CGCCATGGAT GAAGAAGAGA TTGAGAAAGC 3312 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCCAATT CTCAAAATCT TACCATTTCT CGCCATGGAT GAAGAAGAGA TTGAGAAAGC 180 AGTCGTAGCG TATTTGAAGA AGAAGGGTTT CAAGCAAACG GAGATTGCTT TTCAAGAAGA 3372 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTCGTAGCG TATTTGAAGA AGAAGGGTTT CAAGCAAACG GAGATTGCTT TTCAAGAAGA 240 ACAGCAAGTG AATAAAAACA GTTCTTCTTC AATCCATAAT AACAACAACT CAGCAAATTC 3432 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACAGCAAGTG AATAAAAACA GTTCTTCTTC AATCCATAAT AACAACAACT CAGCAAATTC 300 TCAGATTGAC CCCGACTTAA CCAAAAAAAT CCTTTCTTTC TCTGAGTAAG TTTCTTTTTT 3492 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TCAGATTGAC CCCGACTTAA CCAAAAAAAT CCTTTCTTTC TCTGA..... .......... 345 CCACTTAATT TGATGTAAAA ATTACTGTTT GGCTAAGAGG ATTAAGTTCT AAAATCAATT 3552 .......... .......... .......... .......... .......... .......... 345 TACCTTGAAA GATAGTTTAA TTTCAATGAA ACACGTTTAA GTAGAGAAGG ATTGAGTACA 3612 .......... .......... .......... .......... .......... .......... 345 GAAGATCTGA AAACTAGTTT AGGGTTGAGA GGTGACTTGG TTGTTGGAAG AAAAGCAGGA 3672 .......... .......... .......... .......... .......... .......... 345 GCAATATAAT TGAAGCAGTT TTTCTGCACC CTCCAGTAGC ATTTCTGCAA ATGCCCAAAT 3732 .......... .......... .......... .......... .......... .......... 345 TGACCCTGAA CCGTCAGACA AAAATAATCT GTTCTCTGTA GATAACATAA ATTTAATTAC 3792 .......... .......... .......... .......... .......... .......... 345 TGGACCCATT CTAAAGAGGG AGAAATCAGC ACTACTTGGT GAAATAGGTT CTTTTTGAAT 3852 .......... .......... .......... .......... .......... .......... 345 CAATTGTCCT CTTCTCCTGT ATTTTAAATG CAGAGTGCTT AGCCATGAGG TTGCGGTTCT 3912 .......... .......... .......... .......... .......... .......... 345 AAATAGCATT GACAATGCCC AGAACCACTT TTGAGGTACT AGCACACTGT CTAGGAATTG 3972 .......... .......... .......... .......... .......... .......... 345 GAAAGAGAGG ATCAAAGGAA ATTTGGCGGA AGAATATCCC TGTTTGTATC TGGTGGACGT 4032 .......... .......... .......... .......... .......... .......... 345 TATGAAAAAA AAAGGAATGA GAGATATTAT GAAGGGAAGG GTAGCAGCAC ACAGAAGATC 4092 .......... .......... .......... .......... .......... .......... 345 AAGATGAGAT CTCTTAGTAT TATATTTTTT TGGTGTAAGC AAGATTTAGT GGGGACAATA 4152 .......... .......... .......... .......... .......... .......... 345 GATAGCTTAG TGGAGATTAT AGGCCAACTG TAAATTTTTG AAAGTTTGTT TTTGGATTTT 4212 .......... .......... .......... .......... .......... .......... 345 GAGTATGATG GCATCCCCTG GGGTTTGTAA ATTCCTCCTC ATCAGTCTGA TGAGTTCTTT 4272 .......... .......... .......... .......... .......... .......... 345 TGTGCGGATT TAAGTTACCT ATTTCAAAAT AAATAAATAA ATAGAGGGGA TGTAGAGGAG 4332 .......... .......... .......... .......... .......... .......... 345 GATTTATGAG CTGACCCAAA GTCTCAAACT GTTTAGGATT GAGGCTTCAT TAGTTGTTTC 4392 .......... .......... .......... .......... .......... .......... 345 AAGAAGGGAA GCAACAATAA TTGTGTGTTT GGTATGGAGG AAGCTTTGGT GTTATTTTAC 4452 .......... .......... .......... .......... .......... .......... 345 ATTCTATTAG TGACATTACA CTTTGGAGCT TCCTACTTTT AGAATATTAC AGATATAGAT 4512 .......... .......... .......... .......... .......... .......... 345 GGATAGATGT TATGGCTCCG TTTTCACACC GCCTTACACT AGCTGGAAGT CTTGACAGCT 4572 .......... .......... .......... .......... .......... .......... 345 GTATAACGCA TACTTTCTGG TTATTTTCTT AGCATGCATC TTTGATATCA TTCTGAATCT 4632 .......... .......... .......... .......... .......... .......... 345 AAATTTTTAT GTTTTCAGAT TTGAAACCAG TCCCCAACGA TACCAAGAAG AATATAGCAA 4692 || |||||||||| ||||| |||| |||||||||| ||||||||| .......... ........AT TTGAAACCAG TCCCCTACGA TACCAAGAAG AATATAGCAT 387 ACTTCGGTCA TGGGCCTATA GTTCACTGGA TCTGTATAAG GTTGGTCCCC TGACTTGTGC 4752 ||||| |||| |||||||||| |||||||||| |||||||||| ACTTCAGTCA TGGGCCTATA GTTCACTGGA TCTGTATAAG .......... .......... 427 TAACAGTAAC ATGAGAAGTA GTTTATGCAG TATCATGATG CATAGCTCCT CATGACATGT 4812 .......... .......... .......... .......... .......... .......... 427 AATGCACTAA TAATAGGTCT GCCACCAGTA ACACCATAGG AAATAAACAT AAATGGAAAG 4872 .......... .......... .......... .......... .......... .......... 427 GAAAGGCTTT CATTTTCTCT TTTATTTAGC CCTTTTTAAT CCTTAACATA ATGTATTAGT 4932 .......... .......... .......... .......... .......... .......... 427 GATGTGCACC TTGACCTATT AATACTTCTG CTGCTGTCTT TCTGGCAGTG ACAAGGTCTT 4992 .......... .......... .......... .......... .......... .......... 427 CAAAATTGAG TTCTCATATT TGTGATTCTC AACTCTAGTT CATTGACTTA TGCTATAAAT 5052 .......... .......... .......... .......... .......... .......... 427 GTAGCACCAG TGATGGATTT AGTACTGTCT TCTACCATTG CGATCATAAT AGCGAGCCAA 5112 .......... .......... .......... .......... .......... .......... 427 AAATAGCTCT TCATAACATA AGCATGACAA GCGTGCCGTC TTCATACAAG TTAGGTTGTG 5172 .......... .......... .......... .......... .......... .......... 427 TGATTATACA AGCCAGATGA AAAAGTGATG AAAATGGTTC CATATGTTTG GTAGGTTCAA 5232 .......... .......... .......... .......... .......... .......... 427 GTTGGTCCCT TAATACTCTT GAACAATTTT GGTCCTTTAA GTTCACCAAA AGCGAAAACA 5292 .......... .......... .......... .......... .......... .......... 427 CCCTTTGGGG GGGGGGGCAG TGGTTTGGGC TTGGGGCTTC CATTTTGGAG GTTTCAAGTT 5352 .......... .......... .......... .......... .......... .......... 427 CGAAACCCCT TGCTAGCAAA AGCAAGGGTC CAGTTTAGAA AATCAAGAGA TAATTTACCT 5412 .......... .......... .......... .......... .......... .......... 427 TTTGTGTCCT TTGTTATTAA ATACTATTTA TCATCTAACA CATTTTCCAG AGCATTAAAT 5472 .......... .......... .......... .......... .......... .......... 427 TTAATAAAGT CAAAGGATAA AATAGTAATG TTACCGCTAT TATTTATTGT TTCTTAAGGG 5532 .......... .......... .......... .......... .......... .......... 427 TGTGCTAAGT CAATAGTAGA CAACTATTGT TGGACCAAGG GAGTAACATC TATAGTTTCA 5592 .......... .......... .......... .......... .......... .......... 427 ACGTTTGCCA GCTTGCTAAT TTTGGTTTAT TTTTGGAGGA TGGTGCAGCT TTTGAAGTGC 5652 .......... .......... .......... .......... .......... .......... 427 AATTTTGCCA TCTTTTAATA TCTATTGTGT GGTATATGGT TCAATTTTTG GTGTTGCAAT 5712 .......... .......... .......... .......... .......... .......... 427 TTTCAAGATA GGACAGGACT TTTTGGTGTT TTTGGTTGGA TAGTTATTTT GACCATTCCG 5772 .......... .......... .......... .......... .......... .......... 427 ATGAAGTCTA CCCATTCAAG TTCTGTTTAA AAAAAAAAAG GGTCCAACCA CTTGAGTTCT 5832 .......... .......... .......... .......... .......... .......... 427 GTTTCAAAAA AAAAAGTCTT AACCATCCGA GTTTGTTAAA TATTTGTCAG AGACCAAATG 5892 .......... .......... .......... .......... .......... .......... 427 CTCACTTTTG GCAAACTTAG AAAACCCAAA TTGTTGGGGG GTATATTGAA GGACCACCTT 5952 .......... .......... .......... .......... .......... .......... 427 GAACCTATAC TCAAACATAA GGAGCCATTT TTTCTCTCTC TCCAAGTTAG ACACTTCCTT 6012 .......... .......... .......... .......... .......... .......... 427 TTGACTTACT CTTTAATCTT CAAGATTTTG TGGTTTCCTG TAATAGCTGA AGCTACCTTC 6072 .......... .......... .......... .......... .......... .......... 427 CATTTATTTT TGTCCATTTC TGTCTACCTT GTCTCAGTCC ATTCCAGATT GCCTCTCTCT 6132 .......... .......... .......... .......... .......... .......... 427 TTCTATTGGA ATTGAAGGAT GTGCTGTGTG CATCTGGATT TTCTCCAATT CCAACATAAT 6192 .......... .......... .......... .......... .......... .......... 427 CTGATATTGA TTGAAAAGAG GGAAGTTAAG CTAGCTAACG AAGGGGTTGG GATCCGCCTC 6252 .......... .......... .......... .......... .......... .......... 427 GCCCTTAAAG ATCCGCCCCT CGTTTCACTC TGAAAATTTA TTATCAGCTC CTTCTGTTAT 6312 .......... .......... .......... .......... .......... .......... 427 TTAGCCATCT CTCTCCATAA AATTCTTTCA CGTACATGAA AGTTATTTCA TATCCTAGAG 6372 .......... .......... .......... .......... .......... .......... 427 AGTGCGGTTG TATTGTCTCT CTAAAGAATC GTAGGATCCA TTGGAGATAA AATGAAACAA 6432 .......... .......... .......... .......... .......... .......... 427 TTTTAAATGC CGAGTGTTCT GTTTCCTTTT TTTCCTTTGG TCGTTTCTCT TTCTGCAGTG 6492 .......... .......... .......... .......... .......... .......... 427 CTGTTACTGC TTAGAATACC TTTCGGTTCG CAGAAATGAT GTGCAAACTT TGTTGTTTCC 6552 .......... .......... .......... .......... .......... .......... 427 AACAGTTTGG CCATTGTATA TATTGGGGTT GTTAAGTCTT TCTCTTTTAT AGTATGCGTG 6612 .......... .......... .......... .......... .......... .......... 427 ACTCATTTAA AATCATCTTA TATTATGTTG ATTATTGTTA TCCTCTTTTA TTTGTTAGAT 6672 .......... .......... .......... .......... .......... .......... 427 GAGATTATTG TTATTATTGA TTCATCTGAA CATATATATA TTTCAATCTT GCAGCATGAG 6732 |||||| .......... .......... .......... .......... .......... ....CATGAG 433 TTGCTTCGTG TTTTATATCC TGTATTTATT CATTGTTTCA TGGAACTTGT CGCTAGAGGT 6792 |||||||| | | |||||||| |||||||||| ||||| |||| |||||||||| |||||||||| TTGCTTCGAG TATTATATCC TGTATTTATT CATTGCTTCA TGGAACTTGT CGCTAGAGGT 493 TATATTCAAG AAGGTATACT TTGTCAGTTA TGTTTTGGTT GTATTTTGAT ATTTGTGAGA 6852 ||||||| || ||| TATATTCTAG AAG....... .......... .......... .......... .......... 506 GAATTTTTAA CACAATTCTT TTATTTCCAG CACGAGCCTT TTTCAATAGT TACCGTGAAG 6912 |||||||||| |||||||||| |||||||||| .......... .......... .......... CACGAGCCTT TTTCAATAGT TACCGTGAAG 536 ACCATGAGAT GACGCATCTG CGTG 6936 |||||||||| |||||||||| |||| ACCATGAGAT GACGCATCTG CGTG 560 hqPGS_C06HBa0046G10.1-4+_SGN-E542658+ (3133 3477,4651 4732,6727 6805,6883 6936) ******************************************************************************** EST sequence 7 +strand 527 n (File: SGN-E301411+) 1 AATCTTACCA TTTCTCGCCA TGGATGAAGA AGAGATTGAG AAAGCAGTCG TAGCGTATTT 61 GAAGAAGAAG GGTTTCAAGC AAACGGAGAT TGCTTTTCAA GAAGAACAGC AAGTGAATAA 121 AAACAGTTCT TCTTCAATCC ATAATAACAA CAACTCAGCA AATTCTCAGA TTGACCCCGA 181 CTTAACCAAA AAAATCCTTT CTTTCTCTGA ATTTGAAACC AGTCCCCAAC GATACCAAGA 241 AGAATATAGC AAACTTCGGT CATGGGCCTA TAGTTCACTG GATCTGTATA AGCATGAGTT 301 GCTTCGTGTT TTATATCCTG TATTTATTCA TTGTTTCATG GAACTTGTCG CTAGAGGTTA 361 TATTCAAGAA GCACGAGCCT TTTTCAATAG TTACCGTGAA GACCATGAGA TGACGCATCT 421 GCGTGATCTT CAGAAGTTAG AAGGCGTCCT CTCACCTTCC CATCTAGAGG AAATGGAATT 481 TGCTCATTCT ATACGGGTGA GCAAAGTGAA CATTAAGATG TGTCAGT Predicted gene structure (within gDNA segment 2668 to 7742): Exon 1 3268 3477 ( 210 n); cDNA 1 210 ( 210 n); score: 1.000 Intron 1 3478 4650 (1173 n); Pd: 0.998 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 4651 4732 ( 82 n); cDNA 211 292 ( 82 n); score: 1.000 Intron 2 4733 6726 (1994 n); Pd: 0.815 (s: 1.00), Pa: 0.328 (s: 1.00) Exon 3 6727 6805 ( 79 n); cDNA 293 371 ( 79 n); score: 1.000 Intron 3 6806 6882 ( 77 n); Pd: 0.900 (s: 1.00), Pa: 0.975 (s: 1.00) Exon 4 6883 6980 ( 98 n); cDNA 372 469 ( 98 n); score: 1.000 Intron 4 6981 7065 ( 85 n); Pd: 0.979 (s: 1.00), Pa: 0.985 (s: 1.00) Exon 5 7066 7122 ( 57 n); cDNA 470 526 ( 57 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E301411+ 1.000 526 0.998 C PGS_C06HBa0046G10.1-4+_SGN-E301411+ (3268 3477,4651 4732,6727 6805,6883 6980,7066 7122) Alignment (genomic DNA sequence = upper lines): AATCTTACCA TTTCTCGCCA TGGATGAAGA AGAGATTGAG AAAGCAGTCG TAGCGTATTT 3327 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATCTTACCA TTTCTCGCCA TGGATGAAGA AGAGATTGAG AAAGCAGTCG TAGCGTATTT 60 GAAGAAGAAG GGTTTCAAGC AAACGGAGAT TGCTTTTCAA GAAGAACAGC AAGTGAATAA 3387 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGAAGAAG GGTTTCAAGC AAACGGAGAT TGCTTTTCAA GAAGAACAGC AAGTGAATAA 120 AAACAGTTCT TCTTCAATCC ATAATAACAA CAACTCAGCA AATTCTCAGA TTGACCCCGA 3447 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACAGTTCT TCTTCAATCC ATAATAACAA CAACTCAGCA AATTCTCAGA TTGACCCCGA 180 CTTAACCAAA AAAATCCTTT CTTTCTCTGA GTAAGTTTCT TTTTTCCACT TAATTTGATG 3507 |||||||||| |||||||||| |||||||||| CTTAACCAAA AAAATCCTTT CTTTCTCTGA .......... .......... .......... 210 TAAAAATTAC TGTTTGGCTA AGAGGATTAA GTTCTAAAAT CAATTTACCT TGAAAGATAG 3567 .......... .......... .......... .......... .......... .......... 210 TTTAATTTCA ATGAAACACG TTTAAGTAGA GAAGGATTGA GTACAGAAGA TCTGAAAACT 3627 .......... .......... .......... .......... .......... .......... 210 AGTTTAGGGT TGAGAGGTGA CTTGGTTGTT GGAAGAAAAG CAGGAGCAAT ATAATTGAAG 3687 .......... .......... .......... .......... .......... .......... 210 CAGTTTTTCT GCACCCTCCA GTAGCATTTC TGCAAATGCC CAAATTGACC CTGAACCGTC 3747 .......... .......... .......... .......... .......... .......... 210 AGACAAAAAT AATCTGTTCT CTGTAGATAA CATAAATTTA ATTACTGGAC CCATTCTAAA 3807 .......... .......... .......... .......... .......... .......... 210 GAGGGAGAAA TCAGCACTAC TTGGTGAAAT AGGTTCTTTT TGAATCAATT GTCCTCTTCT 3867 .......... .......... .......... .......... .......... .......... 210 CCTGTATTTT AAATGCAGAG TGCTTAGCCA TGAGGTTGCG GTTCTAAATA GCATTGACAA 3927 .......... .......... .......... .......... .......... .......... 210 TGCCCAGAAC CACTTTTGAG GTACTAGCAC ACTGTCTAGG AATTGGAAAG AGAGGATCAA 3987 .......... .......... .......... .......... .......... .......... 210 AGGAAATTTG GCGGAAGAAT ATCCCTGTTT GTATCTGGTG GACGTTATGA AAAAAAAAGG 4047 .......... .......... .......... .......... .......... .......... 210 AATGAGAGAT ATTATGAAGG GAAGGGTAGC AGCACACAGA AGATCAAGAT GAGATCTCTT 4107 .......... .......... .......... .......... .......... .......... 210 AGTATTATAT TTTTTTGGTG TAAGCAAGAT TTAGTGGGGA CAATAGATAG CTTAGTGGAG 4167 .......... .......... .......... .......... .......... .......... 210 ATTATAGGCC AACTGTAAAT TTTTGAAAGT TTGTTTTTGG ATTTTGAGTA TGATGGCATC 4227 .......... .......... .......... .......... .......... .......... 210 CCCTGGGGTT TGTAAATTCC TCCTCATCAG TCTGATGAGT TCTTTTGTGC GGATTTAAGT 4287 .......... .......... .......... .......... .......... .......... 210 TACCTATTTC AAAATAAATA AATAAATAGA GGGGATGTAG AGGAGGATTT ATGAGCTGAC 4347 .......... .......... .......... .......... .......... .......... 210 CCAAAGTCTC AAACTGTTTA GGATTGAGGC TTCATTAGTT GTTTCAAGAA GGGAAGCAAC 4407 .......... .......... .......... .......... .......... .......... 210 AATAATTGTG TGTTTGGTAT GGAGGAAGCT TTGGTGTTAT TTTACATTCT ATTAGTGACA 4467 .......... .......... .......... .......... .......... .......... 210 TTACACTTTG GAGCTTCCTA CTTTTAGAAT ATTACAGATA TAGATGGATA GATGTTATGG 4527 .......... .......... .......... .......... .......... .......... 210 CTCCGTTTTC ACACCGCCTT ACACTAGCTG GAAGTCTTGA CAGCTGTATA ACGCATACTT 4587 .......... .......... .......... .......... .......... .......... 210 TCTGGTTATT TTCTTAGCAT GCATCTTTGA TATCATTCTG AATCTAAATT TTTATGTTTT 4647 .......... .......... .......... .......... .......... .......... 210 CAGATTTGAA ACCAGTCCCC AACGATACCA AGAAGAATAT AGCAAACTTC GGTCATGGGC 4707 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...ATTTGAA ACCAGTCCCC AACGATACCA AGAAGAATAT AGCAAACTTC GGTCATGGGC 267 CTATAGTTCA CTGGATCTGT ATAAGGTTGG TCCCCTGACT TGTGCTAACA GTAACATGAG 4767 |||||||||| |||||||||| ||||| CTATAGTTCA CTGGATCTGT ATAAG..... .......... .......... .......... 292 AAGTAGTTTA TGCAGTATCA TGATGCATAG CTCCTCATGA CATGTAATGC ACTAATAATA 4827 .......... .......... .......... .......... .......... .......... 292 GGTCTGCCAC CAGTAACACC ATAGGAAATA AACATAAATG GAAAGGAAAG GCTTTCATTT 4887 .......... .......... .......... .......... .......... .......... 292 TCTCTTTTAT TTAGCCCTTT TTAATCCTTA ACATAATGTA TTAGTGATGT GCACCTTGAC 4947 .......... .......... .......... .......... .......... .......... 292 CTATTAATAC TTCTGCTGCT GTCTTTCTGG CAGTGACAAG GTCTTCAAAA TTGAGTTCTC 5007 .......... .......... .......... .......... .......... .......... 292 ATATTTGTGA TTCTCAACTC TAGTTCATTG ACTTATGCTA TAAATGTAGC ACCAGTGATG 5067 .......... .......... .......... .......... .......... .......... 292 GATTTAGTAC TGTCTTCTAC CATTGCGATC ATAATAGCGA GCCAAAAATA GCTCTTCATA 5127 .......... .......... .......... .......... .......... .......... 292 ACATAAGCAT GACAAGCGTG CCGTCTTCAT ACAAGTTAGG TTGTGTGATT ATACAAGCCA 5187 .......... .......... .......... .......... .......... .......... 292 GATGAAAAAG TGATGAAAAT GGTTCCATAT GTTTGGTAGG TTCAAGTTGG TCCCTTAATA 5247 .......... .......... .......... .......... .......... .......... 292 CTCTTGAACA ATTTTGGTCC TTTAAGTTCA CCAAAAGCGA AAACACCCTT TGGGGGGGGG 5307 .......... .......... .......... .......... .......... .......... 292 GGCAGTGGTT TGGGCTTGGG GCTTCCATTT TGGAGGTTTC AAGTTCGAAA CCCCTTGCTA 5367 .......... .......... .......... .......... .......... .......... 292 GCAAAAGCAA GGGTCCAGTT TAGAAAATCA AGAGATAATT TACCTTTTGT GTCCTTTGTT 5427 .......... .......... .......... .......... .......... .......... 292 ATTAAATACT ATTTATCATC TAACACATTT TCCAGAGCAT TAAATTTAAT AAAGTCAAAG 5487 .......... .......... .......... .......... .......... .......... 292 GATAAAATAG TAATGTTACC GCTATTATTT ATTGTTTCTT AAGGGTGTGC TAAGTCAATA 5547 .......... .......... .......... .......... .......... .......... 292 GTAGACAACT ATTGTTGGAC CAAGGGAGTA ACATCTATAG TTTCAACGTT TGCCAGCTTG 5607 .......... .......... .......... .......... .......... .......... 292 CTAATTTTGG TTTATTTTTG GAGGATGGTG CAGCTTTTGA AGTGCAATTT TGCCATCTTT 5667 .......... .......... .......... .......... .......... .......... 292 TAATATCTAT TGTGTGGTAT ATGGTTCAAT TTTTGGTGTT GCAATTTTCA AGATAGGACA 5727 .......... .......... .......... .......... .......... .......... 292 GGACTTTTTG GTGTTTTTGG TTGGATAGTT ATTTTGACCA TTCCGATGAA GTCTACCCAT 5787 .......... .......... .......... .......... .......... .......... 292 TCAAGTTCTG TTTAAAAAAA AAAAGGGTCC AACCACTTGA GTTCTGTTTC AAAAAAAAAA 5847 .......... .......... .......... .......... .......... .......... 292 GTCTTAACCA TCCGAGTTTG TTAAATATTT GTCAGAGACC AAATGCTCAC TTTTGGCAAA 5907 .......... .......... .......... .......... .......... .......... 292 CTTAGAAAAC CCAAATTGTT GGGGGGTATA TTGAAGGACC ACCTTGAACC TATACTCAAA 5967 .......... .......... .......... .......... .......... .......... 292 CATAAGGAGC CATTTTTTCT CTCTCTCCAA GTTAGACACT TCCTTTTGAC TTACTCTTTA 6027 .......... .......... .......... .......... .......... .......... 292 ATCTTCAAGA TTTTGTGGTT TCCTGTAATA GCTGAAGCTA CCTTCCATTT ATTTTTGTCC 6087 .......... .......... .......... .......... .......... .......... 292 ATTTCTGTCT ACCTTGTCTC AGTCCATTCC AGATTGCCTC TCTCTTTCTA TTGGAATTGA 6147 .......... .......... .......... .......... .......... .......... 292 AGGATGTGCT GTGTGCATCT GGATTTTCTC CAATTCCAAC ATAATCTGAT ATTGATTGAA 6207 .......... .......... .......... .......... .......... .......... 292 AAGAGGGAAG TTAAGCTAGC TAACGAAGGG GTTGGGATCC GCCTCGCCCT TAAAGATCCG 6267 .......... .......... .......... .......... .......... .......... 292 CCCCTCGTTT CACTCTGAAA ATTTATTATC AGCTCCTTCT GTTATTTAGC CATCTCTCTC 6327 .......... .......... .......... .......... .......... .......... 292 CATAAAATTC TTTCACGTAC ATGAAAGTTA TTTCATATCC TAGAGAGTGC GGTTGTATTG 6387 .......... .......... .......... .......... .......... .......... 292 TCTCTCTAAA GAATCGTAGG ATCCATTGGA GATAAAATGA AACAATTTTA AATGCCGAGT 6447 .......... .......... .......... .......... .......... .......... 292 GTTCTGTTTC CTTTTTTTCC TTTGGTCGTT TCTCTTTCTG CAGTGCTGTT ACTGCTTAGA 6507 .......... .......... .......... .......... .......... .......... 292 ATACCTTTCG GTTCGCAGAA ATGATGTGCA AACTTTGTTG TTTCCAACAG TTTGGCCATT 6567 .......... .......... .......... .......... .......... .......... 292 GTATATATTG GGGTTGTTAA GTCTTTCTCT TTTATAGTAT GCGTGACTCA TTTAAAATCA 6627 .......... .......... .......... .......... .......... .......... 292 TCTTATATTA TGTTGATTAT TGTTATCCTC TTTTATTTGT TAGATGAGAT TATTGTTATT 6687 .......... .......... .......... .......... .......... .......... 292 ATTGATTCAT CTGAACATAT ATATATTTCA ATCTTGCAGC ATGAGTTGCT TCGTGTTTTA 6747 | |||||||||| |||||||||| .......... .......... .......... .........C ATGAGTTGCT TCGTGTTTTA 313 TATCCTGTAT TTATTCATTG TTTCATGGAA CTTGTCGCTA GAGGTTATAT TCAAGAAGGT 6807 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TATCCTGTAT TTATTCATTG TTTCATGGAA CTTGTCGCTA GAGGTTATAT TCAAGAAG.. 371 ATACTTTGTC AGTTATGTTT TGGTTGTATT TTGATATTTG TGAGAGAATT TTTAACACAA 6867 .......... .......... .......... .......... .......... .......... 371 TTCTTTTATT TCCAGCACGA GCCTTTTTCA ATAGTTACCG TGAAGACCAT GAGATGACGC 6927 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....CACGA GCCTTTTTCA ATAGTTACCG TGAAGACCAT GAGATGACGC 416 ATCTGCGTGA TCTTCAGAAG TTAGAAGGCG TCCTCTCACC TTCCCATCTA GAGGTACATC 6987 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| ATCTGCGTGA TCTTCAGAAG TTAGAAGGCG TCCTCTCACC TTCCCATCTA GAG....... 469 AATATCCTAG CAACTGTGTG GATGACCTTT TCCATGCTTA AGTGTGTCAT AGATCACTAA 7047 .......... .......... .......... .......... .......... .......... 469 ACTTATTCCG AAATGCAGGA AATGGAATTT GCTCATTCTA TACGGGTGAG CAAAGTGAAC 7107 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........GA AATGGAATTT GCTCATTCTA TACGGGTGAG CAAAGTGAAC 511 ATTAAGATGT GTCAG 7122 |||||||||| ||||| ATTAAGATGT GTCAG 526 hqPGS_C06HBa0046G10.1-4+_SGN-E301411+ (3268 3477,4651 4732,6727 6805,6883 6980,7066 7122) ******************************************************************************** EST sequence 9 +strand 594 n (File: SGN-E323609+) 1 CCATGGATGA AGAAGAGATT GAGAAAGCAG TCGTAGCGTA TTTGAAGAAG AAGGGTTTCA 61 AGCAAACGGA GATTGCTTTT CAAGAAGAAC AGCAAGTGAA TAAAAACAGT TCTTCTTCAA 121 TCCATAATAA CAACAACTCA GCAAATTCTC AGATTGACCC CGACTTAACC AAAAAAATCC 181 TTTCTTTCTC TGAATTTGAA ACCAGTCCCC AACGATACCA AGAAGAATAT AGCAAACTTC 241 GGTCATGGGC CTATAGTTCA CTGGATCTGT ATAAGCATGA GTTGCTTCGT GTTTTATATC 301 CTGTATTTAT TCATTGTTTC ATGGAACTTG TCGCTAGAGG TTATATTCAA GAAGCACGAG 361 CCTTTTTCAA TAGTTACCGT GAAGACCATG AGATGACGCA TCTGCGTGAT CTTCAGAAGT 421 TAGAAGGCGT CCTCTCACCT TCCCATCTAG AGGAAATGGA ATTTGCTCAT TCTATACGGG 481 TGAGCAAAGT GAACATTAAG ATGTGTCAGT ACTCCTATGA CCTTCTGTTG CAATACTTGC 541 ACAAGACAGA ATCTATTACA ATGCTTGGGA TCATCAATGA GCGCATTAAT TTTC Predicted gene structure (within gDNA segment 2685 to 8391): Exon 1 3285 3477 ( 193 n); cDNA 1 193 ( 193 n); score: 1.000 Intron 1 3478 4650 (1173 n); Pd: 0.998 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 4651 4732 ( 82 n); cDNA 194 275 ( 82 n); score: 1.000 Intron 2 4733 6726 (1994 n); Pd: 0.815 (s: 1.00), Pa: 0.328 (s: 1.00) Exon 3 6727 6805 ( 79 n); cDNA 276 354 ( 79 n); score: 1.000 Intron 3 6806 6882 ( 77 n); Pd: 0.900 (s: 1.00), Pa: 0.975 (s: 1.00) Exon 4 6883 6980 ( 98 n); cDNA 355 452 ( 98 n); score: 1.000 Intron 4 6981 7065 ( 85 n); Pd: 0.979 (s: 1.00), Pa: 0.985 (s: 1.00) Exon 5 7066 7122 ( 57 n); cDNA 453 509 ( 57 n); score: 1.000 Intron 5 7123 7696 ( 574 n); Pd: 0.985 (s: 1.00), Pa: 0.648 (s: 1.00) Exon 6 7697 7781 ( 85 n); cDNA 510 594 ( 85 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E323609+ 1.000 594 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E323609+ (3285 3477,4651 4732,6727 6805,6883 6980,7066 7122,7697 7781) Alignment (genomic DNA sequence = upper lines): CCATGGATGA AGAAGAGATT GAGAAAGCAG TCGTAGCGTA TTTGAAGAAG AAGGGTTTCA 3344 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCATGGATGA AGAAGAGATT GAGAAAGCAG TCGTAGCGTA TTTGAAGAAG AAGGGTTTCA 60 AGCAAACGGA GATTGCTTTT CAAGAAGAAC AGCAAGTGAA TAAAAACAGT TCTTCTTCAA 3404 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCAAACGGA GATTGCTTTT CAAGAAGAAC AGCAAGTGAA TAAAAACAGT TCTTCTTCAA 120 TCCATAATAA CAACAACTCA GCAAATTCTC AGATTGACCC CGACTTAACC AAAAAAATCC 3464 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCATAATAA CAACAACTCA GCAAATTCTC AGATTGACCC CGACTTAACC AAAAAAATCC 180 TTTCTTTCTC TGAGTAAGTT TCTTTTTTCC ACTTAATTTG ATGTAAAAAT TACTGTTTGG 3524 |||||||||| ||| TTTCTTTCTC TGA....... .......... .......... .......... .......... 193 CTAAGAGGAT TAAGTTCTAA AATCAATTTA CCTTGAAAGA TAGTTTAATT TCAATGAAAC 3584 .......... .......... .......... .......... .......... .......... 193 ACGTTTAAGT AGAGAAGGAT TGAGTACAGA AGATCTGAAA ACTAGTTTAG GGTTGAGAGG 3644 .......... .......... .......... .......... .......... .......... 193 TGACTTGGTT GTTGGAAGAA AAGCAGGAGC AATATAATTG AAGCAGTTTT TCTGCACCCT 3704 .......... .......... .......... .......... .......... .......... 193 CCAGTAGCAT TTCTGCAAAT GCCCAAATTG ACCCTGAACC GTCAGACAAA AATAATCTGT 3764 .......... .......... .......... .......... .......... .......... 193 TCTCTGTAGA TAACATAAAT TTAATTACTG GACCCATTCT AAAGAGGGAG AAATCAGCAC 3824 .......... .......... .......... .......... .......... .......... 193 TACTTGGTGA AATAGGTTCT TTTTGAATCA ATTGTCCTCT TCTCCTGTAT TTTAAATGCA 3884 .......... .......... .......... .......... .......... .......... 193 GAGTGCTTAG CCATGAGGTT GCGGTTCTAA ATAGCATTGA CAATGCCCAG AACCACTTTT 3944 .......... .......... .......... .......... .......... .......... 193 GAGGTACTAG CACACTGTCT AGGAATTGGA AAGAGAGGAT CAAAGGAAAT TTGGCGGAAG 4004 .......... .......... .......... .......... .......... .......... 193 AATATCCCTG TTTGTATCTG GTGGACGTTA TGAAAAAAAA AGGAATGAGA GATATTATGA 4064 .......... .......... .......... .......... .......... .......... 193 AGGGAAGGGT AGCAGCACAC AGAAGATCAA GATGAGATCT CTTAGTATTA TATTTTTTTG 4124 .......... .......... .......... .......... .......... .......... 193 GTGTAAGCAA GATTTAGTGG GGACAATAGA TAGCTTAGTG GAGATTATAG GCCAACTGTA 4184 .......... .......... .......... .......... .......... .......... 193 AATTTTTGAA AGTTTGTTTT TGGATTTTGA GTATGATGGC ATCCCCTGGG GTTTGTAAAT 4244 .......... .......... .......... .......... .......... .......... 193 TCCTCCTCAT CAGTCTGATG AGTTCTTTTG TGCGGATTTA AGTTACCTAT TTCAAAATAA 4304 .......... .......... .......... .......... .......... .......... 193 ATAAATAAAT AGAGGGGATG TAGAGGAGGA TTTATGAGCT GACCCAAAGT CTCAAACTGT 4364 .......... .......... .......... .......... .......... .......... 193 TTAGGATTGA GGCTTCATTA GTTGTTTCAA GAAGGGAAGC AACAATAATT GTGTGTTTGG 4424 .......... .......... .......... .......... .......... .......... 193 TATGGAGGAA GCTTTGGTGT TATTTTACAT TCTATTAGTG ACATTACACT TTGGAGCTTC 4484 .......... .......... .......... .......... .......... .......... 193 CTACTTTTAG AATATTACAG ATATAGATGG ATAGATGTTA TGGCTCCGTT TTCACACCGC 4544 .......... .......... .......... .......... .......... .......... 193 CTTACACTAG CTGGAAGTCT TGACAGCTGT ATAACGCATA CTTTCTGGTT ATTTTCTTAG 4604 .......... .......... .......... .......... .......... .......... 193 CATGCATCTT TGATATCATT CTGAATCTAA ATTTTTATGT TTTCAGATTT GAAACCAGTC 4664 |||| |||||||||| .......... .......... .......... .......... ......ATTT GAAACCAGTC 207 CCCAACGATA CCAAGAAGAA TATAGCAAAC TTCGGTCATG GGCCTATAGT TCACTGGATC 4724 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCAACGATA CCAAGAAGAA TATAGCAAAC TTCGGTCATG GGCCTATAGT TCACTGGATC 267 TGTATAAGGT TGGTCCCCTG ACTTGTGCTA ACAGTAACAT GAGAAGTAGT TTATGCAGTA 4784 |||||||| TGTATAAG.. .......... .......... .......... .......... .......... 275 TCATGATGCA TAGCTCCTCA TGACATGTAA TGCACTAATA ATAGGTCTGC CACCAGTAAC 4844 .......... .......... .......... .......... .......... .......... 275 ACCATAGGAA ATAAACATAA ATGGAAAGGA AAGGCTTTCA TTTTCTCTTT TATTTAGCCC 4904 .......... .......... .......... .......... .......... .......... 275 TTTTTAATCC TTAACATAAT GTATTAGTGA TGTGCACCTT GACCTATTAA TACTTCTGCT 4964 .......... .......... .......... .......... .......... .......... 275 GCTGTCTTTC TGGCAGTGAC AAGGTCTTCA AAATTGAGTT CTCATATTTG TGATTCTCAA 5024 .......... .......... .......... .......... .......... .......... 275 CTCTAGTTCA TTGACTTATG CTATAAATGT AGCACCAGTG ATGGATTTAG TACTGTCTTC 5084 .......... .......... .......... .......... .......... .......... 275 TACCATTGCG ATCATAATAG CGAGCCAAAA ATAGCTCTTC ATAACATAAG CATGACAAGC 5144 .......... .......... .......... .......... .......... .......... 275 GTGCCGTCTT CATACAAGTT AGGTTGTGTG ATTATACAAG CCAGATGAAA AAGTGATGAA 5204 .......... .......... .......... .......... .......... .......... 275 AATGGTTCCA TATGTTTGGT AGGTTCAAGT TGGTCCCTTA ATACTCTTGA ACAATTTTGG 5264 .......... .......... .......... .......... .......... .......... 275 TCCTTTAAGT TCACCAAAAG CGAAAACACC CTTTGGGGGG GGGGGCAGTG GTTTGGGCTT 5324 .......... .......... .......... .......... .......... .......... 275 GGGGCTTCCA TTTTGGAGGT TTCAAGTTCG AAACCCCTTG CTAGCAAAAG CAAGGGTCCA 5384 .......... .......... .......... .......... .......... .......... 275 GTTTAGAAAA TCAAGAGATA ATTTACCTTT TGTGTCCTTT GTTATTAAAT ACTATTTATC 5444 .......... .......... .......... .......... .......... .......... 275 ATCTAACACA TTTTCCAGAG CATTAAATTT AATAAAGTCA AAGGATAAAA TAGTAATGTT 5504 .......... .......... .......... .......... .......... .......... 275 ACCGCTATTA TTTATTGTTT CTTAAGGGTG TGCTAAGTCA ATAGTAGACA ACTATTGTTG 5564 .......... .......... .......... .......... .......... .......... 275 GACCAAGGGA GTAACATCTA TAGTTTCAAC GTTTGCCAGC TTGCTAATTT TGGTTTATTT 5624 .......... .......... .......... .......... .......... .......... 275 TTGGAGGATG GTGCAGCTTT TGAAGTGCAA TTTTGCCATC TTTTAATATC TATTGTGTGG 5684 .......... .......... .......... .......... .......... .......... 275 TATATGGTTC AATTTTTGGT GTTGCAATTT TCAAGATAGG ACAGGACTTT TTGGTGTTTT 5744 .......... .......... .......... .......... .......... .......... 275 TGGTTGGATA GTTATTTTGA CCATTCCGAT GAAGTCTACC CATTCAAGTT CTGTTTAAAA 5804 .......... .......... .......... .......... .......... .......... 275 AAAAAAAGGG TCCAACCACT TGAGTTCTGT TTCAAAAAAA AAAGTCTTAA CCATCCGAGT 5864 .......... .......... .......... .......... .......... .......... 275 TTGTTAAATA TTTGTCAGAG ACCAAATGCT CACTTTTGGC AAACTTAGAA AACCCAAATT 5924 .......... .......... .......... .......... .......... .......... 275 GTTGGGGGGT ATATTGAAGG ACCACCTTGA ACCTATACTC AAACATAAGG AGCCATTTTT 5984 .......... .......... .......... .......... .......... .......... 275 TCTCTCTCTC CAAGTTAGAC ACTTCCTTTT GACTTACTCT TTAATCTTCA AGATTTTGTG 6044 .......... .......... .......... .......... .......... .......... 275 GTTTCCTGTA ATAGCTGAAG CTACCTTCCA TTTATTTTTG TCCATTTCTG TCTACCTTGT 6104 .......... .......... .......... .......... .......... .......... 275 CTCAGTCCAT TCCAGATTGC CTCTCTCTTT CTATTGGAAT TGAAGGATGT GCTGTGTGCA 6164 .......... .......... .......... .......... .......... .......... 275 TCTGGATTTT CTCCAATTCC AACATAATCT GATATTGATT GAAAAGAGGG AAGTTAAGCT 6224 .......... .......... .......... .......... .......... .......... 275 AGCTAACGAA GGGGTTGGGA TCCGCCTCGC CCTTAAAGAT CCGCCCCTCG TTTCACTCTG 6284 .......... .......... .......... .......... .......... .......... 275 AAAATTTATT ATCAGCTCCT TCTGTTATTT AGCCATCTCT CTCCATAAAA TTCTTTCACG 6344 .......... .......... .......... .......... .......... .......... 275 TACATGAAAG TTATTTCATA TCCTAGAGAG TGCGGTTGTA TTGTCTCTCT AAAGAATCGT 6404 .......... .......... .......... .......... .......... .......... 275 AGGATCCATT GGAGATAAAA TGAAACAATT TTAAATGCCG AGTGTTCTGT TTCCTTTTTT 6464 .......... .......... .......... .......... .......... .......... 275 TCCTTTGGTC GTTTCTCTTT CTGCAGTGCT GTTACTGCTT AGAATACCTT TCGGTTCGCA 6524 .......... .......... .......... .......... .......... .......... 275 GAAATGATGT GCAAACTTTG TTGTTTCCAA CAGTTTGGCC ATTGTATATA TTGGGGTTGT 6584 .......... .......... .......... .......... .......... .......... 275 TAAGTCTTTC TCTTTTATAG TATGCGTGAC TCATTTAAAA TCATCTTATA TTATGTTGAT 6644 .......... .......... .......... .......... .......... .......... 275 TATTGTTATC CTCTTTTATT TGTTAGATGA GATTATTGTT ATTATTGATT CATCTGAACA 6704 .......... .......... .......... .......... .......... .......... 275 TATATATATT TCAATCTTGC AGCATGAGTT GCTTCGTGTT TTATATCCTG TATTTATTCA 6764 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..CATGAGTT GCTTCGTGTT TTATATCCTG TATTTATTCA 313 TTGTTTCATG GAACTTGTCG CTAGAGGTTA TATTCAAGAA GGTATACTTT GTCAGTTATG 6824 |||||||||| |||||||||| |||||||||| |||||||||| | TTGTTTCATG GAACTTGTCG CTAGAGGTTA TATTCAAGAA G......... .......... 354 TTTTGGTTGT ATTTTGATAT TTGTGAGAGA ATTTTTAACA CAATTCTTTT ATTTCCAGCA 6884 || .......... .......... .......... .......... .......... ........CA 356 CGAGCCTTTT TCAATAGTTA CCGTGAAGAC CATGAGATGA CGCATCTGCG TGATCTTCAG 6944 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGAGCCTTTT TCAATAGTTA CCGTGAAGAC CATGAGATGA CGCATCTGCG TGATCTTCAG 416 AAGTTAGAAG GCGTCCTCTC ACCTTCCCAT CTAGAGGTAC ATCAATATCC TAGCAACTGT 7004 |||||||||| |||||||||| |||||||||| |||||| AAGTTAGAAG GCGTCCTCTC ACCTTCCCAT CTAGAG.... .......... .......... 452 GTGGATGACC TTTTCCATGC TTAAGTGTGT CATAGATCAC TAAACTTATT CCGAAATGCA 7064 .......... .......... .......... .......... .......... .......... 452 GGAAATGGAA TTTGCTCATT CTATACGGGT GAGCAAAGTG AACATTAAGA TGTGTCAGGT 7124 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| .GAAATGGAA TTTGCTCATT CTATACGGGT GAGCAAAGTG AACATTAAGA TGTGTCAG.. 509 ATTCTCTTGA TCCTTTTGCT TCCTCTGGTT GAATTTTTCT GCTACTATAG TGCACACTAA 7184 .......... .......... .......... .......... .......... .......... 509 TGTCTCAACT GTGAAAGCCA AGTGTGTCGG GGTTTGTTAG CAATTGGTGA CAAATGCATA 7244 .......... .......... .......... .......... .......... .......... 509 TCGAGTTAGT AGTTACAAAG GAGGAAAAGA AACTAATGTG ATATTTCACT TTAGAGTGCA 7304 .......... .......... .......... .......... .......... .......... 509 TTATCTTTCA TTTCCTTCTC TTGACCACTT ATTTTGCTGT GGAACTTCAA CTTTTTGACA 7364 .......... .......... .......... .......... .......... .......... 509 AAAAATCCAC CTCTTTCCAA ATCTTTTCTT TTGAAGCTGA ACTTCTATTA GTTATGTTGA 7424 .......... .......... .......... .......... .......... .......... 509 TGAAATAGCA AACACTGGTT CATTTTGTAG GTTTGTTGGA ATTAACTACA ACATTTAGTC 7484 .......... .......... .......... .......... .......... .......... 509 TATTGTTCTC ATGCCATGGA TCTTATCACC ATGTATATTA AAGGAACAAT CATTTGTCTA 7544 .......... .......... .......... .......... .......... .......... 509 ATGGTTTTTC TAGTTTGGTG GATTTACGTG CAGTTTGATT ACTGTTTTTT GGTCAATATA 7604 .......... .......... .......... .......... .......... .......... 509 CATGTCTGGC ACCATTTGAT TACTTTTTAC CACGCATACC TTTAGACTTA ACTGGATTAG 7664 .......... .......... .......... .......... .......... .......... 509 CCTTTTGATT TGCTTATTTT ACTCTGAAAC AGTACTCCTA TGACCTTCTG TTGCAATACT 7724 |||||||| |||||||||| |||||||||| .......... .......... .......... ..TACTCCTA TGACCTTCTG TTGCAATACT 537 TGCACAAGAC AGAATCTATT ACAATGCTTG GGATCATCAA TGAGCGCATT AATTTTC 7781 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TGCACAAGAC AGAATCTATT ACAATGCTTG GGATCATCAA TGAGCGCATT AATTTTC 594 hqPGS_C06HBa0046G10.1-4+_SGN-E323609+ (3285 3477,4651 4732,6727 6805,6883 6980,7066 7122,7697 7781) ******************************************************************************** EST sequence 26 -strand 708 n (File: SGN-E542617-) 1 ATTTGCTCAT TCTATACGGG TGAGCAAAGT GAACATTAAG ATGTGTCAGT ACTCCTATGA 61 CCTTCTGTTG CAATACTTGC ACAAGACAGA ATCTCTTACA ATGCTTGGGA TCATCAATGA 121 GCGCATTATT TTCAAGTTTC TGCTGGACAG CCTGGCTCTA TATCTGGTGA TACGGAAGTT 181 GTAACTCTTG TTACAAGTGG CCATGATGCT AGTCTCATAA ATCAGAAAGA AGTTCTTTGG 241 GGGATGCTTG AAGATTCTTT AGAAGACCGG TTAGAAAAGA CAAGAGGTTC TGTTCCAGAT 301 TCGGAAAAGT TTGATGGAGA TCCGAAGGAA GGAGAAGCGG AAGAAAACAA GAAGAAGTCA 361 ATTGAAGGTG GTAAACATGC TGCACCTCTT AAAAAACTAA AGAAGGACAA AGTTGGGATT 421 GCAACAGGGA AATCTTCTCG TACAGAGAGC AGCACAGTGA CAACTGCACC ACGTGTCAAA 481 CCGGAACTTG CTTTACCTGC TATGTACTCC TCAAACCTAT TATCTTACTT ACCAAAAGAA 541 AAAGAGAGGG CCCTATTGTG TTTTTCTCTC ACCACGACAT CACTCCCTGT ACTTGGAAAA 601 GTCATTCTTG TTGGATGTCA TAATTTTATT TGAAATACAG TCATCATGAT CCATTAGAAA 661 ACATCTAAGT GAAACGGTCT ATACAAAGAA GTTGCATAAA AAAAAAAA Predicted gene structure (within gDNA segment 6464 to 12914): Exon 1 7074 7122 ( 49 n); cDNA 1 49 ( 49 n); score: 1.000 Intron 1 7123 7696 ( 574 n); Pd: 0.985 (s: 1.00), Pa: 0.648 (s: 0.98) Exon 2 7697 7784 ( 88 n); cDNA 50 136 ( 87 n); score: 0.977 Intron 2 7785 9491 (1707 n); Pd: 0.915 (s: 0.96), Pa: 0.976 (s: 0.98) Exon 3 9492 9598 ( 107 n); cDNA 137 243 ( 107 n); score: 0.981 Intron 3 9599 11629 (2031 n); Pd: 0.997 (s: 1.00), Pa: 0.997 (s: 0.94) Exon 4 11630 11737 ( 108 n); cDNA 244 351 ( 108 n); score: 0.963 Intron 4 11738 11916 ( 179 n); Pd: 0.999 (s: 0.98), Pa: 0.912 (s: 0.98) Exon 5 11917 12269 ( 353 n); cDNA 352 708 ( 357 n); score: 0.963 MATCH C06HBa0046G10.1-4+ SGN-E542617- 0.968 705 0.996 C PGS_C06HBa0046G10.1-4+_SGN-E542617- (7074 7122,7697 7784,9492 9598,11630 11737,11917 12269) Alignment (genomic DNA sequence = upper lines): ATTTGCTCAT TCTATACGGG TGAGCAAAGT GAACATTAAG ATGTGTCAGG TATTCTCTTG 7133 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ATTTGCTCAT TCTATACGGG TGAGCAAAGT GAACATTAAG ATGTGTCAG. .......... 49 ATCCTTTTGC TTCCTCTGGT TGAATTTTTC TGCTACTATA GTGCACACTA ATGTCTCAAC 7193 .......... .......... .......... .......... .......... .......... 49 TGTGAAAGCC AAGTGTGTCG GGGTTTGTTA GCAATTGGTG ACAAATGCAT ATCGAGTTAG 7253 .......... .......... .......... .......... .......... .......... 49 TAGTTACAAA GGAGGAAAAG AAACTAATGT GATATTTCAC TTTAGAGTGC ATTATCTTTC 7313 .......... .......... .......... .......... .......... .......... 49 ATTTCCTTCT CTTGACCACT TATTTTGCTG TGGAACTTCA ACTTTTTGAC AAAAAATCCA 7373 .......... .......... .......... .......... .......... .......... 49 CCTCTTTCCA AATCTTTTCT TTTGAAGCTG AACTTCTATT AGTTATGTTG ATGAAATAGC 7433 .......... .......... .......... .......... .......... .......... 49 AAACACTGGT TCATTTTGTA GGTTTGTTGG AATTAACTAC AACATTTAGT CTATTGTTCT 7493 .......... .......... .......... .......... .......... .......... 49 CATGCCATGG ATCTTATCAC CATGTATATT AAAGGAACAA TCATTTGTCT AATGGTTTTT 7553 .......... .......... .......... .......... .......... .......... 49 CTAGTTTGGT GGATTTACGT GCAGTTTGAT TACTGTTTTT TGGTCAATAT ACATGTCTGG 7613 .......... .......... .......... .......... .......... .......... 49 CACCATTTGA TTACTTTTTA CCACGCATAC CTTTAGACTT AACTGGATTA GCCTTTTGAT 7673 .......... .......... .......... .......... .......... .......... 49 TTGCTTATTT TACTCTGAAA CAGTACTCCT ATGACCTTCT GTTGCAATAC TTGCACAAGA 7733 ||||||| |||||||||| |||||||||| |||||||||| .......... .......... ...TACTCCT ATGACCTTCT GTTGCAATAC TTGCACAAGA 86 CAGAATCTAT TACAATGCTT GGGATCATCA ATGAGCGCAT TAATTTTCAA GGTGCACTTC 7793 |||||||| | |||||||||| |||||||||| |||||||||| | |||||||| | CAGAATCTCT TACAATGCTT GGGATCATCA ATGAGCGCAT T-ATTTTCAA G......... 136 CCATTTTCGC AGTTGCTTTC AACCTTTCAA TTTGCAACTA TGTTAAATTG ACCATTTTAA 7853 .......... .......... .......... .......... .......... .......... 136 TTTAGCGTAA TCTTTTTTGA TTAAGTAAAG TATTTCATTA AGAAAACAAG GCCGAGATGA 7913 .......... .......... .......... .......... .......... .......... 136 CTGTAAACAA GAGATATACC CTAAAAGAAG AAACCTACAA AAATATGGTT CACTCTAAAA 7973 .......... .......... .......... .......... .......... .......... 136 TACACCCAAT CCTCTATACT AATAGGAACG ACATGGTGCA CCAAAAGAAA ATAGGGAGTG 8033 .......... .......... .......... .......... .......... .......... 136 GAGACTGCTA CTTAGTTGAG CAAGGCTCAT CTCCATTCCT TCAAAAGCCC CCATATTTCT 8093 .......... .......... .......... .......... .......... .......... 136 CTCTCCAAAT GACCCACGTT AAAGCAAGAG AAGTAACATT CCAAGCCTTT TGCCTTCTTC 8153 .......... .......... .......... .......... .......... .......... 136 TCCTGGCACC AGTTGTCGAA CTAAACATCA ACTCTTTCAC GGATCTTGGC ATCAGCCCTG 8213 .......... .......... .......... .......... .......... .......... 136 AAATGCCGAA CCATCTAAAG ATACCCTACC ATAACCTCAT TGCTAGATTG CAATGTAGCA 8273 .......... .......... .......... .......... .......... .......... 136 TAATATGATC CACGATCTCA CCTGCCTCCT TACTAAGGAA ACACCAACTA ATACAGACAA 8333 .......... .......... .......... .......... .......... .......... 136 CTTTCCATTT CCTAACATTC TCCGCCGTCA AAATCACCCC TCTATAAACT AACCAAGTGA 8393 .......... .......... .......... .......... .......... .......... 136 AGAAGCACCC CTTCCTTGGA ATCCTAGGAA TATCGTAAAT ATTTATTCCA CCATGAAGTG 8453 .......... .......... .......... .......... .......... .......... 136 TTTAGGAAGA AGCAGTTACA TCTTTCCATT TATATTGGTA TGATAGTGAA ACCATATGAG 8513 .......... .......... .......... .......... .......... .......... 136 AAGGAAGAAG AGAAATGTCT TTTCCTTGAC CATTGTCCTT TAGAACAAAT GCTCATCATT 8573 .......... .......... .......... .......... .......... .......... 136 ACATCCACTG AATAAAGACG GGGAAAGGAA AAGCGTTCCA ATTTAAATTC AACTTCTTTC 8633 .......... .......... .......... .......... .......... .......... 136 TGTCAGATCA TTGTTTATTT TGTCCCTCCA TGATCACTTA ACCATCTCAA TCCTCTGGAA 8693 .......... .......... .......... .......... .......... .......... 136 CCAAATTACA TGACTAGAGG GGAACTGTCA TGTTTATTAT AAATAGTGGT GAATTTTACC 8753 .......... .......... .......... .......... .......... .......... 136 ATAATTACTT CCTTTATATT GCTTGGTTCT TCCATAGGTG TGCTCTCTAG CTTTTGTTTC 8813 .......... .......... .......... .......... .......... .......... 136 TTGGTTCAAG GCTTGTTACT AACTCGAACA AACTCCAATA CATTTTCCTT GGTAGCTAAA 8873 .......... .......... .......... .......... .......... .......... 136 ATTTATCAAG TTACAGGGAG AAAAAGAAAT GAGAAAAGAA GTCTTTGATA AGATATTGCA 8933 .......... .......... .......... .......... .......... .......... 136 GAGGTGGCTG TTTTTGTTGC TTAATTGTCA TGTTATCGTG GGAGAACATG GTCACATAGG 8993 .......... .......... .......... .......... .......... .......... 136 AGGTGTAACA AGTGGTGGTT TAACTAGCTA TAGTGACTAT GTGCCTTCAC TGCTGCCTCT 9053 .......... .......... .......... .......... .......... .......... 136 ATTGGGTTCT GACCAAGGGA GGTTGCTAAA ATTAAGGTCA AAGATTTATT GGAATGAAGA 9113 .......... .......... .......... .......... .......... .......... 136 ACAGTAATAA GAGTGAGTTA TAGATGCAGA GGGTATTCCA GATCTATTTA TAGGCCTTGA 9173 .......... .......... .......... .......... .......... .......... 136 ATTCTTTTCC TCTTTATCTC TTTCATTCGG ATTCCACTTT TGCTTGTGCC AAGAAATGCC 9233 .......... .......... .......... .......... .......... .......... 136 AAAGTCACTA GATGCACATG ATTTACTCAA TTAAGTTCAT GGTATGTTTG GTGCAAGGCC 9293 .......... .......... .......... .......... .......... .......... 136 GAAGACATGT TCTTCCCAGG TAGTCTGGCA ATGTTCTAGC AAAAAATTGT CCTAAATGCA 9353 .......... .......... .......... .......... .......... .......... 136 ACCCTATGAA TAGTTTAGTG GCCCATGATC CCCCTGTGCC TTGCCTATTA CTCATTATAT 9413 .......... .......... .......... .......... .......... .......... 136 AATCATATGG TATAATTTTA GAGCTATGTA TTTATTAAAT TTAAGTACAA CATGAAGCTA 9473 .......... .......... .......... .......... .......... .......... 136 ATTTGTATCA AATGACAGTT TCTCCTGGAC AGCCTGGCTC TATATCTGGT GATACGGAAG 9533 || ||| |||||| |||||||||| |||||||||| |||||||||| .......... ........TT TCTGCTGGAC AGCCTGGCTC TATATCTGGT GATACGGAAG 178 TTGTAACTCT TGTTGCAAGT GGCCATGATG CTAGTCTCAT AAATCAGAAA GAAGTTCTTT 9593 |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTAACTCT TGTTACAAGT GGCCATGATG CTAGTCTCAT AAATCAGAAA GAAGTTCTTT 238 GGGGGGTAAG CAAACAGTCA ATACCAATAA TTTAATCTTT ATGTTTTTGG ATTGGATTGT 9653 ||||| GGGGG..... .......... .......... .......... .......... .......... 243 GTAGTAAATA TCAGCTTTTG ACCACTTTAC CTGTCAATTT CTTGAGCTAT TTGATGAATA 9713 .......... .......... .......... .......... .......... .......... 243 TTCTTGTTTG TTGGTTAACT GACCATGAAA CAATTAAATT GGCCAGCTGG AAGTCTTCCC 9773 .......... .......... .......... .......... .......... .......... 243 ATGCCAACGT ATGGAAGAAT GGATACCCCC CCCCCCCCCC CAAAAAAAAA AACAAGAAAC 9833 .......... .......... .......... .......... .......... .......... 243 AATATAACCA ATTCCTTTAA CAGAACGTAG TGCAGTGCAT TGAGTTCTTG AGAGGTAGGG 9893 .......... .......... .......... .......... .......... .......... 243 TAATTAGTGG GAAAATAACA ATTCTTTGGA TTGCATTACC TTTGAAGTAT GTGTTTCAGA 9953 .......... .......... .......... .......... .......... .......... 243 GTATTGAGGA TAAAATCACA TTATGATCTA AAATTGTTCT ATCAAGATCG GTTTTAGGAA 10013 .......... .......... .......... .......... .......... .......... 243 AAAGAAAAGG TGGCCCTATA CAACATTACC AGTTCCCAAA AAAGAAGAAG GGAAAAGGGC 10073 .......... .......... .......... .......... .......... .......... 243 CCTGCAGTTC ATCAAAGTAA TTGGAGACCA TCTGGGTTGG GTCTGGGACT AGTTCACCAC 10133 .......... .......... .......... .......... .......... .......... 243 TATAAAACAT TTCTCTTATT CTATGTTTTA TAGCTATGGC TTATGGGTAT GGCAAAGCGG 10193 .......... .......... .......... .......... .......... .......... 243 TCAATAAAGT GGGTAAACTT ATGGGAGATC AGGGTTCAAG TTCCTGTAGA AACAAAGGCG 10253 .......... .......... .......... .......... .......... .......... 243 CTAGGTGATT TTTTCTCGTC TACCTAAGCT TTGGTGGGCA GATACTGTGT ATATACTGGT 10313 .......... .......... .......... .......... .......... .......... 243 GGAAGGTAGT AGATACAGAT TCATGCAAGT TTACTGAACA ACCCATATTA AATTGGAAAA 10373 .......... .......... .......... .......... .......... .......... 243 AAAAATAAAA CGAAGAGAAT GTGGAGCTGC AAGTTATAGA CAAGTGATAA GCTTACTCCT 10433 .......... .......... .......... .......... .......... .......... 243 TCGTGTATAA ACTAATATTA TCTAAGGAGA GTTTATCACT ATCAATCCTT TGGGGTGGCC 10493 .......... .......... .......... .......... .......... .......... 243 CAGTGGTTTG AGTTTGTGAC TTCCATGTTG GAGGTCTCAA GTTCGAAACC CCTTGCCAGC 10553 .......... .......... .......... .......... .......... .......... 243 GAAAGCAAGG AGTTTGCCTT CTGGGTCGAG CTCGTCGCAC CAGGTTTGTC TAGTGCGTGT 10613 .......... .......... .......... .......... .......... .......... 243 TACCTCTCAT ATGTGGTTTG CGAGCTATTG CATAGGAGCG GGGTTTTTAC CCTGTGCGCA 10673 .......... .......... .......... .......... .......... .......... 243 CCCAAAGGGT AGCGGCTGTG GGTTTCCCTT GTCATAAAAA AAGAAGAAGA AGTATATCAC 10733 .......... .......... .......... .......... .......... .......... 243 TATCAACTAG TTTTAGCCAT TCTAGGTTCC ATGTTTTGTA AGGATTGCTT GGGTTATGCT 10793 .......... .......... .......... .......... .......... .......... 243 CTTGATCACA CTTCCTCAAT GTGGGTGGAA TACATGTTGG ATTTGATTAA AATAAGATGG 10853 .......... .......... .......... .......... .......... .......... 243 GTAATTAAAT GGGTGCAAAC CTAGGAGACA AGTCAAAAAT ACTAGGTGAT TTCTTCTCAT 10913 .......... .......... .......... .......... .......... .......... 243 CTGCCCAAGC TTGGTAGTTG ATTTATCCTG TAACTGTGTT GGTGGGAGAA AGCAAGTACC 10973 .......... .......... .......... .......... .......... .......... 243 TAACGGAATA CTCAAGGTGC ACACAAGTTG TCATAAAACA CCACCATTTT CAAGTTAAAA 11033 .......... .......... .......... .......... .......... .......... 243 AAAATGTATT ATTTGACTTT CATATGGCGA ATGAAGAGGT CAGAGAGACT AGAGTCAAAA 11093 .......... .......... .......... .......... .......... .......... 243 GATAAAGAAT GTCGTGATGG AGTTGTCCTT TCTTTCGCCT TAATCTTCAT CCTCTTTCTT 11153 .......... .......... .......... .......... .......... .......... 243 TTCTTGTAAC TGAGGGTGAG GTGATGCATG GGTTTTCGAA AATTCTTTCG TTCATTATTT 11213 .......... .......... .......... .......... .......... .......... 243 CATCTTCACT TTAGGGAGTG GGCTGAATAT AAAATGGGCT GAATATATAA TACTACAAAA 11273 .......... .......... .......... .......... .......... .......... 243 ATATTGTATT AGGCCTGATG AAATATAGTG GTAATATAAC TTACCTTTCG CGGTGGAACA 11333 .......... .......... .......... .......... .......... .......... 243 GGCCAATCTT ACATTCCATG TGTGTGTGTA TTGGAGCGGA CCAATCCATA TTACAAAGCA 11393 .......... .......... .......... .......... .......... .......... 243 TATTAATGAA TAAAAAACTG AGGTGAAATG ATTATTTCCA TTTTTATTTA GAATCCCCTT 11453 .......... .......... .......... .......... .......... .......... 243 AGCTGTGTTT GATATTCTAT TTGTACCCAC ATATCCTAGA CAGAATTTAA TGAGTCTGAG 11513 .......... .......... .......... .......... .......... .......... 243 TGTCTGACAC TTCCGACACG GATCATACCC CCCGATTTTA GAAGAGTCTG TGTAATGTAG 11573 .......... .......... .......... .......... .......... .......... 243 ATCTTCCGTG TTTTAAAATT TATCCCCAGG AGATGTGTTA ATAATTTATT TTGCAGATGC 11633 |||| .......... .......... .......... .......... .......... ......ATGC 247 TTGAAGATTC TTTAGAAGAA CGGTTAGAAA AGACTGGAGG TTCTGTTCCA GATTCGGAAA 11693 |||||||||| ||||||||| |||||||||| |||| |||| |||||||||| |||||||||| TTGAAGATTC TTTAGAAGAC CGGTTAGAAA AGACAAGAGG TTCTGTTCCA GATTCGGAAA 307 AGTTTGATGG AGATCCGAAG GAAGGAGAAG TGGAAGAAAA CAAGGTATTG AATTTCTATC 11753 |||||||||| |||||||||| |||||||||| ||||||||| |||| AGTTTGATGG AGATCCGAAG GAAGGAGAAG CGGAAGAAAA CAAG...... .......... 351 AGTTCATTTC CCACTTGATC ATATTCATAA ATATTTCGCA CTTAATTATC TCATCTTTCC 11813 .......... .......... .......... .......... .......... .......... 351 TTAGTGGTGT AAATTTTTTT TGGTGGACAT CCTTCCACAT TTACTTTTGT CGAAAAAGTG 11873 .......... .......... .......... .......... .......... .......... 351 ACATCCTTCC ACATTTATTG TTTTTATTTT GCCCTTTTTT CAGAAGAAGT CAATTGAAGG 11933 ||||||| |||||||||| .......... .......... .......... .......... ...AAGAAGT CAATTGAAGG 368 TGGTAAACAT GGTGCACCTC TTAAAAAACT AAAGAAGGAC AAAGTTGGGA TTGCAACAGG 11993 |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTAAACAT GCTGCACCTC TTAAAAAACT AAAGAAGGAC AAAGTTGGGA TTGCAACAGG 428 GAAATCTTCT CGTACAGAGA GCAGCACAGT GACAACTGCA CCACGTGTCA AACCGGAACT 12053 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAATCTTCT CGTACAGAGA GCAGCACAGT GACAACTGCA CCACGTGTCA AACCGGAACT 488 TGCTTTACCT GCTATGTACT CCTCAAACCT ATTATCTTAC TTACCAAAAG AAAAAGAGAG 12113 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTTTACCT GCTATGTACT CCTCAAACCT ATTATCTTAC TTACCAAAAG AAAAAGAGAG 548 GGCCCTATTG TGTTTTTCTC TC-CCACGAC ATCA-TCCCT GTACTTGGAA AAGTCATTCT 12171 |||||||||| |||||||||| || ||||||| |||| ||||| |||||||||| |||||||||| GGCCCTATTG TGTTTTTCTC TCACCACGAC ATCACTCCCT GTACTTGGAA AAGTCATTCT 608 TGTTGGATGT CATAATTTTA TTTGAAATAC AGTCATCATG ATCCATTAGA AAACATC-AA 12230 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || TGTTGGATGT CATAATTTTA TTTGAAATAC AGTCATCATG ATCCATTAGA AAACATCTAA 668 GTGAAACGG- CTATACAAAG AAGTTGCATA AAAAAGGAAA 12269 ||||||||| |||||||||| |||||||||| ||||| ||| GTGAAACGGT CTATACAAAG AAGTTGCATA AAAAAAAAAA 708 hqPGS_C06HBa0046G10.1-4+_SGN-E542617- (7074 7122,7697 7784,9492 9598,11630 11737,11917 12269) ******************************************************************************** EST sequence 27 -strand 531 n (File: SGN-E542657-) 1 ATGATGCTAG TCTCATAAAT CAGAAAGAAG TTCTTTGGGG GATGCTTGAA GATTCTTTAG 61 AAGACCGGTT AGAAAAGACA GGAGGTTCTG TTCCAGATTC GGAAAAGTTT GATGGAGATC 121 CGAAGGAAGG AGAAGTGGAA GAAAACAAGA AGAAGTCAAT TGAAGGTGGT AAACATGGTG 181 CACCTCTTAA AAAACTAAAG AAGGACAAAG TTGGGATTGC AACAGGGAAA TCTTCTCGTA 241 CAGAGAGCAG CACAGTGACA ACTGCACCAC GTGTCAAACC GGAACTTGCT TTACCTGCTA 301 TGTACTCCTC AAACCTATTA TCTTACTTAC CAAAAGAAAA AGAGAGGGCC CTATTGTGTT 361 TTTCTCTCCC ACGACATCAT CCCTGTACTT GGAAAAGTCA TTCTTGTTGG ATGTCATAAT 421 TTTATTTGAA ATACAGTCAT CATGATCCAT TAGAAAACAT CAAGTGAAAC GGCTATACAA 481 AGAAGTTGCA TAAAAAAAAA AAAAAAAAAA CTCGAGGGGG GCCCGTACCC A Predicted gene structure (within gDNA segment 8948 to 13204): Exon 1 9558 9598 ( 41 n); cDNA 1 41 ( 41 n); score: 1.000 Intron 1 9599 11629 (2031 n); Pd: 0.997 (s: 1.00), Pa: 0.997 (s: 0.96) Exon 2 11630 11737 ( 108 n); cDNA 42 149 ( 108 n); score: 0.981 Intron 2 11738 11916 ( 179 n); Pd: 0.999 (s: 1.00), Pa: 0.912 (s: 1.00) Exon 3 11917 12269 ( 353 n); cDNA 150 502 ( 353 n); score: 0.994 MATCH C06HBa0046G10.1-4+ SGN-E542657- 0.991 502 0.945 C PGS_C06HBa0046G10.1-4+_SGN-E542657- (9558 9598,11630 11737,11917 12269) Alignment (genomic DNA sequence = upper lines): ATGATGCTAG TCTCATAAAT CAGAAAGAAG TTCTTTGGGG GGTAAGCAAA CAGTCAATAC 9617 |||||||||| |||||||||| |||||||||| |||||||||| | ATGATGCTAG TCTCATAAAT CAGAAAGAAG TTCTTTGGGG G......... .......... 41 CAATAATTTA ATCTTTATGT TTTTGGATTG GATTGTGTAG TAAATATCAG CTTTTGACCA 9677 .......... .......... .......... .......... .......... .......... 41 CTTTACCTGT CAATTTCTTG AGCTATTTGA TGAATATTCT TGTTTGTTGG TTAACTGACC 9737 .......... .......... .......... .......... .......... .......... 41 ATGAAACAAT TAAATTGGCC AGCTGGAAGT CTTCCCATGC CAACGTATGG AAGAATGGAT 9797 .......... .......... .......... .......... .......... .......... 41 ACCCCCCCCC CCCCCCCAAA AAAAAAAACA AGAAACAATA TAACCAATTC CTTTAACAGA 9857 .......... .......... .......... .......... .......... .......... 41 ACGTAGTGCA GTGCATTGAG TTCTTGAGAG GTAGGGTAAT TAGTGGGAAA ATAACAATTC 9917 .......... .......... .......... .......... .......... .......... 41 TTTGGATTGC ATTACCTTTG AAGTATGTGT TTCAGAGTAT TGAGGATAAA ATCACATTAT 9977 .......... .......... .......... .......... .......... .......... 41 GATCTAAAAT TGTTCTATCA AGATCGGTTT TAGGAAAAAG AAAAGGTGGC CCTATACAAC 10037 .......... .......... .......... .......... .......... .......... 41 ATTACCAGTT CCCAAAAAAG AAGAAGGGAA AAGGGCCCTG CAGTTCATCA AAGTAATTGG 10097 .......... .......... .......... .......... .......... .......... 41 AGACCATCTG GGTTGGGTCT GGGACTAGTT CACCACTATA AAACATTTCT CTTATTCTAT 10157 .......... .......... .......... .......... .......... .......... 41 GTTTTATAGC TATGGCTTAT GGGTATGGCA AAGCGGTCAA TAAAGTGGGT AAACTTATGG 10217 .......... .......... .......... .......... .......... .......... 41 GAGATCAGGG TTCAAGTTCC TGTAGAAACA AAGGCGCTAG GTGATTTTTT CTCGTCTACC 10277 .......... .......... .......... .......... .......... .......... 41 TAAGCTTTGG TGGGCAGATA CTGTGTATAT ACTGGTGGAA GGTAGTAGAT ACAGATTCAT 10337 .......... .......... .......... .......... .......... .......... 41 GCAAGTTTAC TGAACAACCC ATATTAAATT GGAAAAAAAA ATAAAACGAA GAGAATGTGG 10397 .......... .......... .......... .......... .......... .......... 41 AGCTGCAAGT TATAGACAAG TGATAAGCTT ACTCCTTCGT GTATAAACTA ATATTATCTA 10457 .......... .......... .......... .......... .......... .......... 41 AGGAGAGTTT ATCACTATCA ATCCTTTGGG GTGGCCCAGT GGTTTGAGTT TGTGACTTCC 10517 .......... .......... .......... .......... .......... .......... 41 ATGTTGGAGG TCTCAAGTTC GAAACCCCTT GCCAGCGAAA GCAAGGAGTT TGCCTTCTGG 10577 .......... .......... .......... .......... .......... .......... 41 GTCGAGCTCG TCGCACCAGG TTTGTCTAGT GCGTGTTACC TCTCATATGT GGTTTGCGAG 10637 .......... .......... .......... .......... .......... .......... 41 CTATTGCATA GGAGCGGGGT TTTTACCCTG TGCGCACCCA AAGGGTAGCG GCTGTGGGTT 10697 .......... .......... .......... .......... .......... .......... 41 TCCCTTGTCA TAAAAAAAGA AGAAGAAGTA TATCACTATC AACTAGTTTT AGCCATTCTA 10757 .......... .......... .......... .......... .......... .......... 41 GGTTCCATGT TTTGTAAGGA TTGCTTGGGT TATGCTCTTG ATCACACTTC CTCAATGTGG 10817 .......... .......... .......... .......... .......... .......... 41 GTGGAATACA TGTTGGATTT GATTAAAATA AGATGGGTAA TTAAATGGGT GCAAACCTAG 10877 .......... .......... .......... .......... .......... .......... 41 GAGACAAGTC AAAAATACTA GGTGATTTCT TCTCATCTGC CCAAGCTTGG TAGTTGATTT 10937 .......... .......... .......... .......... .......... .......... 41 ATCCTGTAAC TGTGTTGGTG GGAGAAAGCA AGTACCTAAC GGAATACTCA AGGTGCACAC 10997 .......... .......... .......... .......... .......... .......... 41 AAGTTGTCAT AAAACACCAC CATTTTCAAG TTAAAAAAAA TGTATTATTT GACTTTCATA 11057 .......... .......... .......... .......... .......... .......... 41 TGGCGAATGA AGAGGTCAGA GAGACTAGAG TCAAAAGATA AAGAATGTCG TGATGGAGTT 11117 .......... .......... .......... .......... .......... .......... 41 GTCCTTTCTT TCGCCTTAAT CTTCATCCTC TTTCTTTTCT TGTAACTGAG GGTGAGGTGA 11177 .......... .......... .......... .......... .......... .......... 41 TGCATGGGTT TTCGAAAATT CTTTCGTTCA TTATTTCATC TTCACTTTAG GGAGTGGGCT 11237 .......... .......... .......... .......... .......... .......... 41 GAATATAAAA TGGGCTGAAT ATATAATACT ACAAAAATAT TGTATTAGGC CTGATGAAAT 11297 .......... .......... .......... .......... .......... .......... 41 ATAGTGGTAA TATAACTTAC CTTTCGCGGT GGAACAGGCC AATCTTACAT TCCATGTGTG 11357 .......... .......... .......... .......... .......... .......... 41 TGTGTATTGG AGCGGACCAA TCCATATTAC AAAGCATATT AATGAATAAA AAACTGAGGT 11417 .......... .......... .......... .......... .......... .......... 41 GAAATGATTA TTTCCATTTT TATTTAGAAT CCCCTTAGCT GTGTTTGATA TTCTATTTGT 11477 .......... .......... .......... .......... .......... .......... 41 ACCCACATAT CCTAGACAGA ATTTAATGAG TCTGAGTGTC TGACACTTCC GACACGGATC 11537 .......... .......... .......... .......... .......... .......... 41 ATACCCCCCG ATTTTAGAAG AGTCTGTGTA ATGTAGATCT TCCGTGTTTT AAAATTTATC 11597 .......... .......... .......... .......... .......... .......... 41 CCCAGGAGAT GTGTTAATAA TTTATTTTGC AGATGCTTGA AGATTCTTTA GAAGAACGGT 11657 |||||||| |||||||||| ||||| |||| .......... .......... .......... ..ATGCTTGA AGATTCTTTA GAAGACCGGT 69 TAGAAAAGAC TGGAGGTTCT GTTCCAGATT CGGAAAAGTT TGATGGAGAT CCGAAGGAAG 11717 |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGAAAAGAC AGGAGGTTCT GTTCCAGATT CGGAAAAGTT TGATGGAGAT CCGAAGGAAG 129 GAGAAGTGGA AGAAAACAAG GTATTGAATT TCTATCAGTT CATTTCCCAC TTGATCATAT 11777 |||||||||| |||||||||| GAGAAGTGGA AGAAAACAAG .......... .......... .......... .......... 149 TCATAAATAT TTCGCACTTA ATTATCTCAT CTTTCCTTAG TGGTGTAAAT TTTTTTTGGT 11837 .......... .......... .......... .......... .......... .......... 149 GGACATCCTT CCACATTTAC TTTTGTCGAA AAAGTGACAT CCTTCCACAT TTATTGTTTT 11897 .......... .......... .......... .......... .......... .......... 149 TATTTTGCCC TTTTTTCAGA AGAAGTCAAT TGAAGGTGGT AAACATGGTG CACCTCTTAA 11957 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........A AGAAGTCAAT TGAAGGTGGT AAACATGGTG CACCTCTTAA 190 AAAACTAAAG AAGGACAAAG TTGGGATTGC AACAGGGAAA TCTTCTCGTA CAGAGAGCAG 12017 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAACTAAAG AAGGACAAAG TTGGGATTGC AACAGGGAAA TCTTCTCGTA CAGAGAGCAG 250 CACAGTGACA ACTGCACCAC GTGTCAAACC GGAACTTGCT TTACCTGCTA TGTACTCCTC 12077 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACAGTGACA ACTGCACCAC GTGTCAAACC GGAACTTGCT TTACCTGCTA TGTACTCCTC 310 AAACCTATTA TCTTACTTAC CAAAAGAAAA AGAGAGGGCC CTATTGTGTT TTTCTCTCCC 12137 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACCTATTA TCTTACTTAC CAAAAGAAAA AGAGAGGGCC CTATTGTGTT TTTCTCTCCC 370 ACGACATCAT CCCTGTACTT GGAAAAGTCA TTCTTGTTGG ATGTCATAAT TTTATTTGAA 12197 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGACATCAT CCCTGTACTT GGAAAAGTCA TTCTTGTTGG ATGTCATAAT TTTATTTGAA 430 ATACAGTCAT CATGATCCAT TAGAAAACAT CAAGTGAAAC GGCTATACAA AGAAGTTGCA 12257 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATACAGTCAT CATGATCCAT TAGAAAACAT CAAGTGAAAC GGCTATACAA AGAAGTTGCA 490 TAAAAAAGGA AA 12269 ||||||| | || TAAAAAAAAA AA 502 hqPGS_C06HBa0046G10.1-4+_SGN-E542657- (9558 9598,11630 11737,11917 12269) ******************************************************************************** EST sequence 29 -strand 661 n (File: SGN-E322543-) 1 AAGAAAAAGT ATACTGGATC CACCAAAGAT GAAGGAGATA GTNGTATCAG TTATAACATG 61 CATGATCTAG AGTAACGATG TGAAAGTGAG ACATTCAAAT CACTTTACCA GATACTAACA 121 TAACGCTAAC AGGTAAAGTT AAATTGAGGA GTAGTCCATT AATGTCGTTA ACCTTGCAAG 181 CAGCTGTCAC GCGTATTCTA TTTTGCCTCT TCATACTCGG TCACCCTTTT CAGAACTAAC 241 TCTTACCGGT TTTTAACTGC ACATTACTTA CTTGTCCAGC ATCAACTTGT GAAGTTACGG 301 ATGGAGGTGG TTCAGAACAT GCCCTCAAGA CAGCTTCATT TATATAAAGC TAAGAGAATG 361 TTACCCTTCG GGGTGGCCCA GTGGTTTGAG CTTGGGACTT CCATGTAGGA GGTCTCAAGT 421 TCGAAACCCC TTGCCAGTGA AAGCAAGGGG TTTGCCTTTT GATCGAGCTC GTCGCACCAA 481 GCTTGCCTAG TGCGGGTTAC CTCTCCTATG TGGTTTGCGA GCTATTGCAT AGGAGCAGGG 541 ATTTTACTCT GTGCGCATCC AAAGGGTAGC GGCTGCGGGT TTCCCTTGTC GTCAAAAAAG 601 GAAAAAAAAA AGCTAAGAGA ATGTTCTCAT TTTACAGTTC AGAGGTTAGA TTTGAAGGAG 661 A Predicted gene structure (within gDNA segment 5585 to 12502): Exon 1 6727 6746 ( 20 n); cDNA 326 343 ( 18 n); score: 0.650 Intron 1 6747 10377 (3631 n); Pd: 0.900 (s: 0), Pa: 0.000 (s: 0) Exon 2 10378 10401 ( 24 n); cDNA 344 367 ( 24 n); score: 0.750 Intron 2 10402 10482 ( 81 n); Pd: 0.427 (s: 0), Pa: 0.000 (s: 0.92) Exon 3 10483 10724 ( 242 n); cDNA 368 608 ( 241 n); score: 0.901 MATCH C06HBa0046G10.1-4+ SGN-E322543- 0.901 286 0.433 C PGS_C06HBa0046G10.1-4+_SGN-E322543- (6727 6746,10378 10401,10483 10724) Alignment (genomic DNA sequence = upper lines): CATGAGTTGC TTCGTGTTTT ATATCCTGTA TTTATTCATT GTTTCATGGA ACTTGTCGCT 6786 || || || ||| | || | CAAGA-CAGC TTCAT-TTAT .......... .......... .......... .......... 343 AGAGGTTATA TTCAAGAAGG TATACTTTGT CAGTTATGTT TTGGTTGTAT TTTGATATTT 6846 .......... .......... .......... .......... .......... .......... 343 GTGAGAGAAT TTTTAACACA ATTCTTTTAT TTCCAGCACG AGCCTTTTTC AATAGTTACC 6906 .......... .......... .......... .......... .......... .......... 343 GTGAAGACCA TGAGATGACG CATCTGCGTG ATCTTCAGAA GTTAGAAGGC GTCCTCTCAC 6966 .......... .......... .......... .......... .......... .......... 343 CTTCCCATCT AGAGGTACAT CAATATCCTA GCAACTGTGT GGATGACCTT TTCCATGCTT 7026 .......... .......... .......... .......... .......... .......... 343 AAGTGTGTCA TAGATCACTA AACTTATTCC GAAATGCAGG AAATGGAATT TGCTCATTCT 7086 .......... .......... .......... .......... .......... .......... 343 ATACGGGTGA GCAAAGTGAA CATTAAGATG TGTCAGGTAT TCTCTTGATC CTTTTGCTTC 7146 .......... .......... .......... .......... .......... .......... 343 CTCTGGTTGA ATTTTTCTGC TACTATAGTG CACACTAATG TCTCAACTGT GAAAGCCAAG 7206 .......... .......... .......... .......... .......... .......... 343 TGTGTCGGGG TTTGTTAGCA ATTGGTGACA AATGCATATC GAGTTAGTAG TTACAAAGGA 7266 .......... .......... .......... .......... .......... .......... 343 GGAAAAGAAA CTAATGTGAT ATTTCACTTT AGAGTGCATT ATCTTTCATT TCCTTCTCTT 7326 .......... .......... .......... .......... .......... .......... 343 GACCACTTAT TTTGCTGTGG AACTTCAACT TTTTGACAAA AAATCCACCT CTTTCCAAAT 7386 .......... .......... .......... .......... .......... .......... 343 CTTTTCTTTT GAAGCTGAAC TTCTATTAGT TATGTTGATG AAATAGCAAA CACTGGTTCA 7446 .......... .......... .......... .......... .......... .......... 343 TTTTGTAGGT TTGTTGGAAT TAACTACAAC ATTTAGTCTA TTGTTCTCAT GCCATGGATC 7506 .......... .......... .......... .......... .......... .......... 343 TTATCACCAT GTATATTAAA GGAACAATCA TTTGTCTAAT GGTTTTTCTA GTTTGGTGGA 7566 .......... .......... .......... .......... .......... .......... 343 TTTACGTGCA GTTTGATTAC TGTTTTTTGG TCAATATACA TGTCTGGCAC CATTTGATTA 7626 .......... .......... .......... .......... .......... .......... 343 CTTTTTACCA CGCATACCTT TAGACTTAAC TGGATTAGCC TTTTGATTTG CTTATTTTAC 7686 .......... .......... .......... .......... .......... .......... 343 TCTGAAACAG TACTCCTATG ACCTTCTGTT GCAATACTTG CACAAGACAG AATCTATTAC 7746 .......... .......... .......... .......... .......... .......... 343 AATGCTTGGG ATCATCAATG AGCGCATTAA TTTTCAAGGT GCACTTCCCA TTTTCGCAGT 7806 .......... .......... .......... .......... .......... .......... 343 TGCTTTCAAC CTTTCAATTT GCAACTATGT TAAATTGACC ATTTTAATTT AGCGTAATCT 7866 .......... .......... .......... .......... .......... .......... 343 TTTTTGATTA AGTAAAGTAT TTCATTAAGA AAACAAGGCC GAGATGACTG TAAACAAGAG 7926 .......... .......... .......... .......... .......... .......... 343 ATATACCCTA AAAGAAGAAA CCTACAAAAA TATGGTTCAC TCTAAAATAC ACCCAATCCT 7986 .......... .......... .......... .......... .......... .......... 343 CTATACTAAT AGGAACGACA TGGTGCACCA AAAGAAAATA GGGAGTGGAG ACTGCTACTT 8046 .......... .......... .......... .......... .......... .......... 343 AGTTGAGCAA GGCTCATCTC CATTCCTTCA AAAGCCCCCA TATTTCTCTC TCCAAATGAC 8106 .......... .......... .......... .......... .......... .......... 343 CCACGTTAAA GCAAGAGAAG TAACATTCCA AGCCTTTTGC CTTCTTCTCC TGGCACCAGT 8166 .......... .......... .......... .......... .......... .......... 343 TGTCGAACTA AACATCAACT CTTTCACGGA TCTTGGCATC AGCCCTGAAA TGCCGAACCA 8226 .......... .......... .......... .......... .......... .......... 343 TCTAAAGATA CCCTACCATA ACCTCATTGC TAGATTGCAA TGTAGCATAA TATGATCCAC 8286 .......... .......... .......... .......... .......... .......... 343 GATCTCACCT GCCTCCTTAC TAAGGAAACA CCAACTAATA CAGACAACTT TCCATTTCCT 8346 .......... .......... .......... .......... .......... .......... 343 AACATTCTCC GCCGTCAAAA TCACCCCTCT ATAAACTAAC CAAGTGAAGA AGCACCCCTT 8406 .......... .......... .......... .......... .......... .......... 343 CCTTGGAATC CTAGGAATAT CGTAAATATT TATTCCACCA TGAAGTGTTT AGGAAGAAGC 8466 .......... .......... .......... .......... .......... .......... 343 AGTTACATCT TTCCATTTAT ATTGGTATGA TAGTGAAACC ATATGAGAAG GAAGAAGAGA 8526 .......... .......... .......... .......... .......... .......... 343 AATGTCTTTT CCTTGACCAT TGTCCTTTAG AACAAATGCT CATCATTACA TCCACTGAAT 8586 .......... .......... .......... .......... .......... .......... 343 AAAGACGGGG AAAGGAAAAG CGTTCCAATT TAAATTCAAC TTCTTTCTGT CAGATCATTG 8646 .......... .......... .......... .......... .......... .......... 343 TTTATTTTGT CCCTCCATGA TCACTTAACC ATCTCAATCC TCTGGAACCA AATTACATGA 8706 .......... .......... .......... .......... .......... .......... 343 CTAGAGGGGA ACTGTCATGT TTATTATAAA TAGTGGTGAA TTTTACCATA ATTACTTCCT 8766 .......... .......... .......... .......... .......... .......... 343 TTATATTGCT TGGTTCTTCC ATAGGTGTGC TCTCTAGCTT TTGTTTCTTG GTTCAAGGCT 8826 .......... .......... .......... .......... .......... .......... 343 TGTTACTAAC TCGAACAAAC TCCAATACAT TTTCCTTGGT AGCTAAAATT TATCAAGTTA 8886 .......... .......... .......... .......... .......... .......... 343 CAGGGAGAAA AAGAAATGAG AAAAGAAGTC TTTGATAAGA TATTGCAGAG GTGGCTGTTT 8946 .......... .......... .......... .......... .......... .......... 343 TTGTTGCTTA ATTGTCATGT TATCGTGGGA GAACATGGTC ACATAGGAGG TGTAACAAGT 9006 .......... .......... .......... .......... .......... .......... 343 GGTGGTTTAA CTAGCTATAG TGACTATGTG CCTTCACTGC TGCCTCTATT GGGTTCTGAC 9066 .......... .......... .......... .......... .......... .......... 343 CAAGGGAGGT TGCTAAAATT AAGGTCAAAG ATTTATTGGA ATGAAGAACA GTAATAAGAG 9126 .......... .......... .......... .......... .......... .......... 343 TGAGTTATAG ATGCAGAGGG TATTCCAGAT CTATTTATAG GCCTTGAATT CTTTTCCTCT 9186 .......... .......... .......... .......... .......... .......... 343 TTATCTCTTT CATTCGGATT CCACTTTTGC TTGTGCCAAG AAATGCCAAA GTCACTAGAT 9246 .......... .......... .......... .......... .......... .......... 343 GCACATGATT TACTCAATTA AGTTCATGGT ATGTTTGGTG CAAGGCCGAA GACATGTTCT 9306 .......... .......... .......... .......... .......... .......... 343 TCCCAGGTAG TCTGGCAATG TTCTAGCAAA AAATTGTCCT AAATGCAACC CTATGAATAG 9366 .......... .......... .......... .......... .......... .......... 343 TTTAGTGGCC CATGATCCCC CTGTGCCTTG CCTATTACTC ATTATATAAT CATATGGTAT 9426 .......... .......... .......... .......... .......... .......... 343 AATTTTAGAG CTATGTATTT ATTAAATTTA AGTACAACAT GAAGCTAATT TGTATCAAAT 9486 .......... .......... .......... .......... .......... .......... 343 GACAGTTTCT CCTGGACAGC CTGGCTCTAT ATCTGGTGAT ACGGAAGTTG TAACTCTTGT 9546 .......... .......... .......... .......... .......... .......... 343 TGCAAGTGGC CATGATGCTA GTCTCATAAA TCAGAAAGAA GTTCTTTGGG GGGTAAGCAA 9606 .......... .......... .......... .......... .......... .......... 343 ACAGTCAATA CCAATAATTT AATCTTTATG TTTTTGGATT GGATTGTGTA GTAAATATCA 9666 .......... .......... .......... .......... .......... .......... 343 GCTTTTGACC ACTTTACCTG TCAATTTCTT GAGCTATTTG ATGAATATTC TTGTTTGTTG 9726 .......... .......... .......... .......... .......... .......... 343 GTTAACTGAC CATGAAACAA TTAAATTGGC CAGCTGGAAG TCTTCCCATG CCAACGTATG 9786 .......... .......... .......... .......... .......... .......... 343 GAAGAATGGA TACCCCCCCC CCCCCCCCAA AAAAAAAAAC AAGAAACAAT ATAACCAATT 9846 .......... .......... .......... .......... .......... .......... 343 CCTTTAACAG AACGTAGTGC AGTGCATTGA GTTCTTGAGA GGTAGGGTAA TTAGTGGGAA 9906 .......... .......... .......... .......... .......... .......... 343 AATAACAATT CTTTGGATTG CATTACCTTT GAAGTATGTG TTTCAGAGTA TTGAGGATAA 9966 .......... .......... .......... .......... .......... .......... 343 AATCACATTA TGATCTAAAA TTGTTCTATC AAGATCGGTT TTAGGAAAAA GAAAAGGTGG 10026 .......... .......... .......... .......... .......... .......... 343 CCCTATACAA CATTACCAGT TCCCAAAAAA GAAGAAGGGA AAAGGGCCCT GCAGTTCATC 10086 .......... .......... .......... .......... .......... .......... 343 AAAGTAATTG GAGACCATCT GGGTTGGGTC TGGGACTAGT TCACCACTAT AAAACATTTC 10146 .......... .......... .......... .......... .......... .......... 343 TCTTATTCTA TGTTTTATAG CTATGGCTTA TGGGTATGGC AAAGCGGTCA ATAAAGTGGG 10206 .......... .......... .......... .......... .......... .......... 343 TAAACTTATG GGAGATCAGG GTTCAAGTTC CTGTAGAAAC AAAGGCGCTA GGTGATTTTT 10266 .......... .......... .......... .......... .......... .......... 343 TCTCGTCTAC CTAAGCTTTG GTGGGCAGAT ACTGTGTATA TACTGGTGGA AGGTAGTAGA 10326 .......... .......... .......... .......... .......... .......... 343 TACAGATTCA TGCAAGTTTA CTGAACAACC CATATTAAAT TGGAAAAAAA AATAAAACGA 10386 ||||| | | .......... .......... .......... .......... .......... .ATAAAGCTA 352 AGAGAATGTG GAGCTGCAAG TTATAGACAA GTGATAAGCT TACTCCTTCG TGTATAAACT 10446 ||||||||| || AGAGAATGTT ACCCT..... .......... .......... .......... .......... 367 AATATTATCT AAGGAGAGTT TATCACTATC AATCCTTTGG GGTGGCCCAG TGGTTTGAGT 10506 | || |||||||||| ||||||||| .......... .......... .......... ......TCGG GGTGGCCCAG TGGTTTGAGC 391 TTGTGACTTC CATGTTGGAG GTCTCAAGTT CGAAACCCCT TGCCAGCGAA AGCAAGGAGT 10566 ||| |||||| ||||| |||| |||||||||| |||||||||| |||||| ||| ||||||| || TTGGGACTTC CATGTAGGAG GTCTCAAGTT CGAAACCCCT TGCCAGTGAA AGCAAGGGGT 451 TTGCCTTCTG GGTCGAGCTC GTCGCACCAG GTTTGTCTAG TGCGTGTTAC CTCTCATATG 10626 ||||||| | | |||||||| ||||||||| | ||| |||| |||| ||||| ||||| |||| TTGCCTT-TT GATCGAGCTC GTCGCACCAA GCTTGCCTAG TGCGGGTTAC CTCTCCTATG 510 TGGTTTGCGA GCTATTGCAT AGGAGCGGGG TTTTTACCCT GTGCGCACCC AAAGGGTAGC 10686 |||||||||| |||||||||| |||||| ||| |||||| || ||||||| || |||||||||| TGGTTTGCGA GCTATTGCAT AGGAGCAGGG ATTTTACTCT GTGCGCATCC AAAGGGTAGC 570 GGCTGTGGGT TTCCCTTGTC ATAAAAAAAG AAGAAGAA 10724 ||||| |||| |||||||||| | ||||||| | || || GGCTGCGGGT TTCCCTTGTC GTCAAAAAAG GAAAAAAA 608 hqPGS_C06HBa0046G10.1-4+_SGN-E322543- (10378 10401,10483 10724) ******************************************************************************** EST sequence 3 +strand 717 n (File: SGN-E380042+) 1 AACGATCATT TTTTCTTTTG ATGATATGCT GAATTATTTT AGTTGAAATG ATGATTTTAG 61 GGAAGTTTGC TCTGCTTAAT TATGATGAAC CCTTCGGGGT GGCCCAGTGG TTTGAGTTTG 121 GATTTTCCAT GCGGGAGGTC TCAAGTTCGA AACTCCTTGC CAGGGAAAGC AAGGGGTTTG 181 CCTTTTGGGT CGAGCTTGTC GCTCCAGGCT TGTCTAGTGC GGTTACCTCT CCTATGTGGT 241 TACCTCTCCT ATGTGGTTTG CGAGTTATTG CATAGGAGTG GGGGTTCTAC CCTGTGCGCA 301 TACAAAGGGT AGCGGCTGCG GGTTTCCCTT GTCATAAAAA TAAATAAATT ATGATGATAC 361 AGTTCTCTTC TATGTATCCT GTCAGGCATT AGACCTTGGA AGAGGTGTGA ATCCAGGAAG 421 ATATATTGTT GGAGAGATCT GGGAAGAGCT TGCTAAAGCA AAGTACATGG AGTGGGAGCA 481 TGAATCTACA AGGCGCTCCT GGGAGCTTCA GAACTTGAAA GAATCATGTG AGTCAGCTCT 541 CAAGGAGAGA CATATGCTTG ACAGTTCTCA GACAGAAGGG TTCAAAGATG AAAAATCAAC 601 GGCGCTTTTT AAGCAACTGA AAGCTCTAGG TAAGGTTTTC GTGAAAGCTG CAGCAGATNG 661 ATACTCCACT GAGGTTCCCG ATTACTTGTG TTGTAAAATT AGTCTTGATA TTTTTCG Predicted gene structure (within gDNA segment 8935 to 15693): Exon 1 10465 10724 ( 260 n); cDNA 76 349 ( 274 n); score: 0.719 Intron 1 10725 14996 (4272 n); Pd: 0.762 (s: 0.86), Pa: 0.976 (s: 0) Exon 2 14997 15001 ( 5 n); cDNA 350 354 ( 5 n); score: 0.600 PPA cDNA 19 9 MATCH C06HBa0046G10.1-4+ SGN-E380042+ 0.719 265 0.370 C PGS_C06HBa0046G10.1-4+_SGN-E380042+ (10465 10724,14997 15001) Alignment (genomic DNA sequence = upper lines): TTTATCACTA TCAATCCTTT GGGGTGGCCC AGTGGTTTGA GTTTGTGACT TCCATGTTGG 10524 || || | | | || |||| |||||||||| |||||||||| ||||| | |||||| || TTAATTATGA TGAACCCTTC GGGGTGGCCC AGTGGTTTGA GTTTGGATTT TCCATGCGGG 135 AGGTCTCAAG TTCGAAACCC CTTGCCAGCG AAAGCAAGGA GTTTGCCTTC TGGGTCGAGC 10584 |||||||||| |||||||| | |||||||| | ||||||||| ||||||||| |||||||||| AGGTCTCAAG TTCGAAACTC CTTGCCAGGG AAAGCAAGGG GTTTGCCTTT TGGGTCGAGC 195 TCGTCGCACC AGGTTTGTCT AGTGC-G--- ---------T ---GTTACCT CTCATATGTG 10628 | ||||| || ||| |||||| ||||| | | ||||||| ||| |||||| TTGTCGCTCC AGGCTTGTCT AGTGCGGTTA CCTCTCCTAT GTGGTTACCT CTCCTATGTG 255 GTTTGCGAGC TATTGCATAG GAGCGGGGTT TTTACCCTGT GCGCACCCAA AGGGTAGCGG 10688 ||||||||| |||||||||| ||| |||| | | |||||||| ||||| ||| |||||||||| GTTTGCGAGT TATTGCATAG GAGTGGGGGT TCTACCCTGT GCGCATACAA AGGGTAGCGG 315 CTGTGGGTTT CCCTTGTCAT AAAAAAAGAA GAAGAAGTAT ATCACTATCA ACTAGTTTTA 10748 ||| |||||| |||||||||| ||||| | || || | CTGCGGGTTT CCCTTGTCAT AAAAATA-AA TAA-AT.... .......... .......... 349 GCCATTCTAG GTTCCATGTT TTGTAAGGAT TGCTTGGGTT ATGCTCTTGA TCACACTTCC 10808 .......... .......... .......... .......... .......... .......... 349 TCAATGTGGG TGGAATACAT GTTGGATTTG ATTAAAATAA GATGGGTAAT TAAATGGGTG 10868 .......... .......... .......... .......... .......... .......... 349 CAAACCTAGG AGACAAGTCA AAAATACTAG GTGATTTCTT CTCATCTGCC CAAGCTTGGT 10928 .......... .......... .......... .......... .......... .......... 349 AGTTGATTTA TCCTGTAACT GTGTTGGTGG GAGAAAGCAA GTACCTAACG GAATACTCAA 10988 .......... .......... .......... .......... .......... .......... 349 GGTGCACACA AGTTGTCATA AAACACCACC ATTTTCAAGT TAAAAAAAAT GTATTATTTG 11048 .......... .......... .......... .......... .......... .......... 349 ACTTTCATAT GGCGAATGAA GAGGTCAGAG AGACTAGAGT CAAAAGATAA AGAATGTCGT 11108 .......... .......... .......... .......... .......... .......... 349 GATGGAGTTG TCCTTTCTTT CGCCTTAATC TTCATCCTCT TTCTTTTCTT GTAACTGAGG 11168 .......... .......... .......... .......... .......... .......... 349 GTGAGGTGAT GCATGGGTTT TCGAAAATTC TTTCGTTCAT TATTTCATCT TCACTTTAGG 11228 .......... .......... .......... .......... .......... .......... 349 GAGTGGGCTG AATATAAAAT GGGCTGAATA TATAATACTA CAAAAATATT GTATTAGGCC 11288 .......... .......... .......... .......... .......... .......... 349 TGATGAAATA TAGTGGTAAT ATAACTTACC TTTCGCGGTG GAACAGGCCA ATCTTACATT 11348 .......... .......... .......... .......... .......... .......... 349 CCATGTGTGT GTGTATTGGA GCGGACCAAT CCATATTACA AAGCATATTA ATGAATAAAA 11408 .......... .......... .......... .......... .......... .......... 349 AACTGAGGTG AAATGATTAT TTCCATTTTT ATTTAGAATC CCCTTAGCTG TGTTTGATAT 11468 .......... .......... .......... .......... .......... .......... 349 TCTATTTGTA CCCACATATC CTAGACAGAA TTTAATGAGT CTGAGTGTCT GACACTTCCG 11528 .......... .......... .......... .......... .......... .......... 349 ACACGGATCA TACCCCCCGA TTTTAGAAGA GTCTGTGTAA TGTAGATCTT CCGTGTTTTA 11588 .......... .......... .......... .......... .......... .......... 349 AAATTTATCC CCAGGAGATG TGTTAATAAT TTATTTTGCA GATGCTTGAA GATTCTTTAG 11648 .......... .......... .......... .......... .......... .......... 349 AAGAACGGTT AGAAAAGACT GGAGGTTCTG TTCCAGATTC GGAAAAGTTT GATGGAGATC 11708 .......... .......... .......... .......... .......... .......... 349 CGAAGGAAGG AGAAGTGGAA GAAAACAAGG TATTGAATTT CTATCAGTTC ATTTCCCACT 11768 .......... .......... .......... .......... .......... .......... 349 TGATCATATT CATAAATATT TCGCACTTAA TTATCTCATC TTTCCTTAGT GGTGTAAATT 11828 .......... .......... .......... .......... .......... .......... 349 TTTTTTGGTG GACATCCTTC CACATTTACT TTTGTCGAAA AAGTGACATC CTTCCACATT 11888 .......... .......... .......... .......... .......... .......... 349 TATTGTTTTT ATTTTGCCCT TTTTTCAGAA GAAGTCAATT GAAGGTGGTA AACATGGTGC 11948 .......... .......... .......... .......... .......... .......... 349 ACCTCTTAAA AAACTAAAGA AGGACAAAGT TGGGATTGCA ACAGGGAAAT CTTCTCGTAC 12008 .......... .......... .......... .......... .......... .......... 349 AGAGAGCAGC ACAGTGACAA CTGCACCACG TGTCAAACCG GAACTTGCTT TACCTGCTAT 12068 .......... .......... .......... .......... .......... .......... 349 GTACTCCTCA AACCTATTAT CTTACTTACC AAAAGAAAAA GAGAGGGCCC TATTGTGTTT 12128 .......... .......... .......... .......... .......... .......... 349 TTCTCTCCCA CGACATCATC CCTGTACTTG GAAAAGTCAT TCTTGTTGGA TGTCATAATT 12188 .......... .......... .......... .......... .......... .......... 349 TTATTTGAAA TACAGTCATC ATGATCCATT AGAAAACATC AAGTGAAACG GCTATACAAA 12248 .......... .......... .......... .......... .......... .......... 349 GAAGTTGCAT AAAAAAGGAA AATTATGAAG GATGAGGAAA ATATGATTGA GCTCAATCCT 12308 .......... .......... .......... .......... .......... .......... 349 TTCTGTGGTG TCATACTCAT AGTACTCCCT CCATTTCAAT TTATTTGACC TTTTTTCCTC 12368 .......... .......... .......... .......... .......... .......... 349 TCTAGTCCAT TGAAAAAAGA ATGACCCTTT CCTTTTTATA GCAACTTCTA AATTTTACTT 12428 .......... .......... .......... .......... .......... .......... 349 CCCAAGTTGT ATGTTTAAGA CTACAAGAAT AAAGGGCATT TCGGTACATT TGACAGATCT 12488 .......... .......... .......... .......... .......... .......... 349 TTGATTTTAA ACCACAAGAT TTAAAGATCT TTATTTTATT AAACTCTGTG CCAAGTCAAA 12548 .......... .......... .......... .......... .......... .......... 349 ACAAGAATTT GAAATGGAGG GAGTGTATTT TTTTAGATAA TTAAATATCT TTAATAAAGT 12608 .......... .......... .......... .......... .......... .......... 349 CATACTCGGT ATATCTTCTT ACATTCATCC ATATATTGCA TTATGTTTCT TATCTCTCTG 12668 .......... .......... .......... .......... .......... .......... 349 TGTGTGTGGG GGGGGGGGGG TTGGATCTTG GATCATCTTA ACTGAAAGAA AATTTGTCAC 12728 .......... .......... .......... .......... .......... .......... 349 AGACCTGTAG AGGTAGAACT TTCTATTCTG GAAGACTTGA GGAACCGCGT ACAGTTGAGT 12788 .......... .......... .......... .......... .......... .......... 349 AATGCATCAT TGCCCTCTGT TAGCTTCTAT ACATTTGTTA ATACACACAA TGGGTAAGTT 12848 .......... .......... .......... .......... .......... .......... 349 TTCATGATCT TGAATCTTTT AAGTGTGGAA TTCGTATTCA GGAACATATC CTAATCTCAG 12908 .......... .......... .......... .......... .......... .......... 349 TGAGTTAATC TGTAAATGTC TGTAGACTAA ACTGTGCATC AATATCACAT GATGGATCAC 12968 .......... .......... .......... .......... .......... .......... 349 TAGTGGCTGG TGGATTCTCA GATTCATCTG TGAAGGTGCT TGTAGCTTCT GAATGACGTT 13028 .......... .......... .......... .......... .......... .......... 349 GAAATTATGT TGTCACAAAT TACAGTATCT GTTGATCCTT GATCATCTTC TTATCTTTTT 13088 .......... .......... .......... .......... .......... .......... 349 TTTGGAACTG GTAACTTTGA TCATCTTCTA ATCTAAGTCC CCCATTTTAC AGGTCTGGGA 13148 .......... .......... .......... .......... .......... .......... 349 CATGGCAAAG CTTGGGGAAC AAACTGGTCC ATGTAAGTCC AATATGATGG TCCTCAGCTC 13208 .......... .......... .......... .......... .......... .......... 349 CTTTTCAATG TATTGTTATT TCATGTTTCA AGATGTTTTA GTGAATAGAG GTTGAAAAGG 13268 .......... .......... .......... .......... .......... .......... 349 TCCCTTTTAA TACTACTCCT AGTCACAGTA TATCTCGCCA GGACAGCAAG AAAGATTTAT 13328 .......... .......... .......... .......... .......... .......... 349 TCTTTGCTGC TTTAATTTAT TTGATAAGTA GATAAATTTT ATTTCACTAA GAAACTGCAC 13388 .......... .......... .......... .......... .......... .......... 349 TAGGGTTGTG AAGTTTGTTA CATCAGAAAT TTGCTTAGCA GAAACATGAT TAGTTATGAA 13448 .......... .......... .......... .......... .......... .......... 349 GGTTTCTCGA CTGAACATAT AAATGCAGCT TTACATTTCT GATGGAAGTA TTTCTTGAAC 13508 .......... .......... .......... .......... .......... .......... 349 TCCTTGCATT TTTCAATTAG GTAAATAATT TTAATCGAAA AGTCTCCTGT ATACAATGCA 13568 .......... .......... .......... .......... .......... .......... 349 GTACACAAAA AATAACTATA TCAGAATATG ATTCTAACAA ACAATATCCT ATTGTTTTTT 13628 .......... .......... .......... .......... .......... .......... 349 TTTTGGAGAA AGTATCAGAA TATGATTCTA ACAAACAATA TCCTACTGTT TTTTGTTTTT 13688 .......... .......... .......... .......... .......... .......... 349 TTTGAGAAAG TAACATTTGT ATTAAAGAAA CTTGGTAAAA AGGTTGTGCT GGTAACCATG 13748 .......... .......... .......... .......... .......... .......... 349 TGCAGCTTCC AAAAGTATTG GGAATCCTTT TACTTCTCAC TAGGGATCTA TCATGTCATA 13808 .......... .......... .......... .......... .......... .......... 349 TTGGATTCTA CATCATTTAC ATAATCTCTT TGCACCAAAA ACATAAACAA CACAAAACAA 13868 .......... .......... .......... .......... .......... .......... 349 TTCATCTCTA TCATTTGAAT GAGGCTATTG CTGCCTTCAA AACATCTAGG GTTCCTTTCC 13928 .......... .......... .......... .......... .......... .......... 349 TTCAAAGCTG TCAACCAAAT GCTGGAGTGA GATCTCCCTG ATTGTGCCAA CATCTCAGTA 13988 .......... .......... .......... .......... .......... .......... 349 AATCAGCTGT AGTTCTTGGC ACTGTCCATC CGAGTCCAAC TATGCTTGGG AAGAGTTGCT 14048 .......... .......... .......... .......... .......... .......... 349 ACAATTGAGC AGTTACACCA AGATGTAAGA ACAGGTGTCT GTTAGACTCT TTAAGTCTGC 14108 .......... .......... .......... .......... .......... .......... 349 ACAGTATTAC TAACATCTGG TGCTCAACTG GAATCCTCTC TTCTTTAAAT TTATCGAGTT 14168 .......... .......... .......... .......... .......... .......... 349 AGACTGGCCT ATAAGAACTT CACTAATGCA CCAAAAAGAA TCAAGGGATT GAAAAGCTAT 14228 .......... .......... .......... .......... .......... .......... 349 TCCTTGAGTC TACATACTCG TTTTCTATCT CTTAAAAAGT CTTGAACCAC TCCTATAATG 14288 .......... .......... .......... .......... .......... .......... 349 TTGAAGTGAT CAATAATTGA GAATTTTGTC ACATGTCTTG TTATGACGCT CATCATGTAT 14348 .......... .......... .......... .......... .......... .......... 349 GCTAGTGGTT TAGATACTCC TAAGGAAAAG GCAAACATTT TGAATCCAAA TGTTAACATG 14408 .......... .......... .......... .......... .......... .......... 349 AGGTTTTTTT TTGAGGATAG TAACACTAAT CTAAATATTC ACAGCTAGAC TACATCCAAA 14468 .......... .......... .......... .......... .......... .......... 349 ATATGTAGTC CCCTTCAAAA GGTATTGCAG TCTAGTAATA TTGCCGTCTA TCACCTATAT 14528 .......... .......... .......... .......... .......... .......... 349 TTCTTACAAA TAATCCAAGA CATCCCAGAT ATCTTGCGCT TGGACTAAAA AGTTCTCTTT 14588 .......... .......... .......... .......... .......... .......... 349 TCACCAAAAA TAAAATAAAG CTAGAGAATT CATCTTGAAG CTCTGAAGTC CATTCTGCTT 14648 .......... .......... .......... .......... .......... .......... 349 GTCTTCAAAG CACCTCTGGT TAGAGTTTTC TCAAAAGGCC TGCATATCCA CTATGGTTCA 14708 .......... .......... .......... .......... .......... .......... 349 GGGCCGATCA TAATCCCTAT GATGTGATAA TTCCGTTGAA TAAGGTGGAA TGTAAGTAAA 14768 .......... .......... .......... .......... .......... .......... 349 AGAAATTCAT TTATAAATAC CAATTAAGTG CCAAACAATG CGTAGATGTC CATGTTACTT 14828 .......... .......... .......... .......... .......... .......... 349 GGAAAAGTTT CCATATGATG CTATATTTCA TGTTCTTTAT TCATTCTTTG TTATTTCGTA 14888 .......... .......... .......... .......... .......... .......... 349 TTAATTATTT ACTTCTTATT ACAGCCTTTT ATATAGTTGA GCTTGTGCTA AGTTGTTTAT 14948 .......... .......... .......... .......... .......... .......... 349 TCTTTTGCAC CATCAGTAAT ATTCTGAATG TTGCATGTGA TGTATCAGGT TGA 15001 ||| .......... .......... .......... .......... ........TA TGA 354 hqPGS_C06HBa0046G10.1-4+_SGN-E380042+ (10465 10724) ******************************************************************************** EST sequence 17 +strand 567 n (File: SGN-E209785+) 1 AACGATCATT TTTTCTTTTG ATGATATGCT GAATTATTTT AGTTGAAATG ATGATTTTAG 61 GGAAGTTTGC TCTGCTTAAT TATGATGAAC CCTTCGGGGT GGCCCAGTGG TTTGAGTTTG 121 GATTTTCCAT GCGGGAGGTC TCAAGTTCGA AACTCCTTGC CAGGGAAAGC AAGGGGTTTG 181 CCTTTTGGGT CGAGCTTGTC GCTCCAGGCT TGTCTAGTGC GGTTACCTCT CCTATGTGGT 241 TACCTCTCCT ATGTGGTTTG CGAGTTATTG CATAGGAGTG GGGGTTCTAC CCTGTGCGCA 301 TACAAAGGGT AGCGGCTGCG GGTTTCCCTT GTCATAAAAA TAAATAAATT ATGATGATAC 361 AGTTCTCTTC TATGTATCCT GTCAGGCATT AGACCTTGGA AGAGGTGTGA ATCCAGGAAG 421 ATATATTGTT GGAGAGATCT GGGAAGAGCT TGCTAAAGCA AAGTACATGG AGTGGGAGCA 481 TGAATCTACA AGGCGCTCCT GGGAGCTTCA GAACTTGAAA GAATCATGTG AGTCAGCTCT 541 CAAGGAGAGA CATATGCTTG ACAGTTC Predicted gene structure (within gDNA segment 8935 to 13593): Exon 1 10465 10721 ( 257 n); cDNA 76 348 ( 273 n); score: 0.720 PPA cDNA 19 9 MATCH C06HBa0046G10.1-4+ SGN-E209785+ 0.720 257 0.453 C PGS_C06HBa0046G10.1-4+_SGN-E209785+ (10465 10721) Alignment (genomic DNA sequence = upper lines): TTTATCACTA TCAATCCTTT GGGGTGGCCC AGTGGTTTGA GTTTGTGACT TCCATGTTGG 10524 || || | | | || |||| |||||||||| |||||||||| ||||| | |||||| || TTAATTATGA TGAACCCTTC GGGGTGGCCC AGTGGTTTGA GTTTGGATTT TCCATGCGGG 135 AGGTCTCAAG TTCGAAACCC CTTGCCAGCG AAAGCAAGGA GTTTGCCTTC TGGGTCGAGC 10584 |||||||||| |||||||| | |||||||| | ||||||||| ||||||||| |||||||||| AGGTCTCAAG TTCGAAACTC CTTGCCAGGG AAAGCAAGGG GTTTGCCTTT TGGGTCGAGC 195 TCGTCGCACC AGGTTTGTCT AGTGC-G--- ---------T ---GTTACCT CTCATATGTG 10628 | ||||| || ||| |||||| ||||| | | ||||||| ||| |||||| TTGTCGCTCC AGGCTTGTCT AGTGCGGTTA CCTCTCCTAT GTGGTTACCT CTCCTATGTG 255 GTTTGCGAGC TATTGCATAG GAGCGGGGTT TTTACCCTGT GCGCACCCAA AGGGTAGCGG 10688 ||||||||| |||||||||| ||| |||| | | |||||||| ||||| ||| |||||||||| GTTTGCGAGT TATTGCATAG GAGTGGGGGT TCTACCCTGT GCGCATACAA AGGGTAGCGG 315 CTGTGGGTTT CCCTTGTCAT AAAAAAAGAA GAA 10721 ||| |||||| |||||||||| ||||| | | || CTGCGGGTTT CCCTTGTCAT AAAAATAAAT AAA 348 hqPGS_C06HBa0046G10.1-4+_SGN-E209785+ (10465 10721) ******************************************************************************** EST sequence 1 +strand 799 n (File: SGN-E348402+) 1 GAAGTTATGT TTTCTTTTTT ATTTAATTAA TATAGGGTGA AAATCATTTT ATCTCTTCTA 61 CATGACTGAG TCTTTCAATG CTTTTGTGGT AAAAGCTAGA GAGAAATTGT TCTAAATTAT 121 TGGAGTAGAT TAGAAGAAAA ACTACGAGCA GATTTTAACA GAGGTATGAA ATAGCTTCTG 181 GTTTGCACAC TCTGAATCCA CCAAAAGTAA GACAAAGAGT TACTCAAAAT CAGGGGGAGG 241 GAATCCTTCG GGGTGGCCCA GTGGTTTGAG CTTGAAACTT CCATGTTGGA GGTCTCAAGT 301 TCGAAAACCC CTGTCAGCAA AAGCAAGGGG TTCTGGGTCG AGCTCGTTGG ACCATGCTTG 361 CCTTGTGTGG GTTACCTCGC CTATGTGGTT TGTGAGCTAT TGCATAGGAG CGGGGTTTTA 421 CCCTGTGCAC AAACTCATAT TAGAGAGGAT CCTACCAAAT TTGTTCATCC TCTACTCAAT 481 GAAGACTGCT ATGTGAAGAC TTATTCTAGA AAGATCAATC CAATACTGGA AGAGAGCAAT 541 GGCCAGTTGT AGATCACACN TGTTGTGCAC CCTAATAAGG AAATGGAGGA GAGGTCGAAA 601 ACCAATTACA TGGAGAAGAG AATTAGATGA ACAACCAGCC AAAAAGAGAG CAATTCATTG 661 CACTTGCAAA ATATGCCATC AAATTGGGCA TTAGTAGTGG GGAAGAAAAC ATCAAGTAAA 721 AACGCACTTC TGCTCATTNG CACGTTGCAT ACCTTGATCC ACTGCTGTAT GGNNTAATGT 781 TTGCTGGCTG CAGTTTGAG Predicted gene structure (within gDNA segment 7395 to 17281): Exon 1 10477 10670 ( 194 n); cDNA 242 428 ( 187 n); score: 0.866 Intron 1 10671 11372 ( 702 n); Pd: 0.000 (s: 0.94), Pa: 0.000 (s: 0) Exon 2 11373 11398 ( 26 n); cDNA 429 454 ( 26 n); score: 0.692 Intron 2 11399 11492 ( 94 n); Pd: 0.000 (s: 0), Pa: 0.497 (s: 0) Exon 3 11493 11506 ( 14 n); cDNA 455 467 ( 13 n); score: 0.643 Intron 3 11507 12203 ( 697 n); Pd: 0.000 (s: 0), Pa: 0.977 (s: 0) Exon 4 12204 12208 ( 5 n); cDNA 468 472 ( 5 n); score: 0.800 Intron 4 12209 13569 (1361 n); Pd: 0.000 (s: 0), Pa: 0.113 (s: 0.58) Exon 5 13570 13611 ( 42 n); cDNA 473 514 ( 42 n); score: 0.583 Intron 5 13612 13938 ( 327 n); Pd: 0.900 (s: 0.58), Pa: 0.000 (s: 0) Exon 6 13939 13954 ( 16 n); cDNA 515 530 ( 16 n); score: 0.812 Intron 6 13955 14996 (1042 n); Pd: 0.111 (s: 0), Pa: 0.976 (s: 0) Exon 7 14997 15003 ( 7 n); cDNA 531 537 ( 7 n); score: 0.429 Intron 7 15004 16616 (1613 n); Pd: 0.165 (s: 0), Pa: 0.000 (s: 0) Exon 8 16617 16633 ( 17 n); cDNA 538 554 ( 17 n); score: 0.765 MATCH C06HBa0046G10.1-4+ SGN-E348402+ 0.866 321 0.402 C PGS_C06HBa0046G10.1-4+_SGN-E348402+ (10477 10670,11373 11398,11493 11506,12204 12208,13570 13611,13939 13954,14997 15003,16617 16633) Alignment (genomic DNA sequence = upper lines): AATCCTTTGG GGTGGCCCAG TGGTTTGAGT TTGTGACTTC CATGTTGGAG GTCTCAAGTT 10536 ||||||| || |||||||||| ||||||||| ||| ||||| |||||||||| |||||||||| AATCCTTCGG GGTGGCCCAG TGGTTTGAGC TTGAAACTTC CATGTTGGAG GTCTCAAGTT 301 CGAAACCCCT TGCCAGCGAA AGCAAGGAGT TTGCCTTCTG GGTCGAGCTC GTCGCACCAG 10596 ||||| ||| || |||| || ||||||| | | ||||| |||||||||| || | |||| CGAAAACCCC TGTCAGCAAA AGCAAGG-G- --G--TTCTG GGTCGAGCTC GTTGGACCAT 355 GTTTGTCTAG TGCGTGTTAC CTCTCATATG TGGTTTGCGA GCTATTGCAT AGGAGCGGGG 10656 | ||| || | || | ||||| ||| | |||| ||||||| || |||||||||| |||||||||| GCTTGCCTTG TGTGGGTTAC CTCGCCTATG TGGTTTGTGA GCTATTGCAT AGGAGCGGGG 415 TTTTTACCCT GTGCGCACCC AAAGGGTAGC GGCTGTGGGT TTCCCTTGTC ATAAAAAAAG 10716 ||||||||| |||| -TTTTACCCT GTGC...... .......... .......... .......... .......... 428 AAGAAGAAGT ATATCACTAT CAACTAGTTT TAGCCATTCT AGGTTCCATG TTTTGTAAGG 10776 .......... .......... .......... .......... .......... .......... 428 ATTGCTTGGG TTATGCTCTT GATCACACTT CCTCAATGTG GGTGGAATAC ATGTTGGATT 10836 .......... .......... .......... .......... .......... .......... 428 TGATTAAAAT AAGATGGGTA ATTAAATGGG TGCAAACCTA GGAGACAAGT CAAAAATACT 10896 .......... .......... .......... .......... .......... .......... 428 AGGTGATTTC TTCTCATCTG CCCAAGCTTG GTAGTTGATT TATCCTGTAA CTGTGTTGGT 10956 .......... .......... .......... .......... .......... .......... 428 GGGAGAAAGC AAGTACCTAA CGGAATACTC AAGGTGCACA CAAGTTGTCA TAAAACACCA 11016 .......... .......... .......... .......... .......... .......... 428 CCATTTTCAA GTTAAAAAAA ATGTATTATT TGACTTTCAT ATGGCGAATG AAGAGGTCAG 11076 .......... .......... .......... .......... .......... .......... 428 AGAGACTAGA GTCAAAAGAT AAAGAATGTC GTGATGGAGT TGTCCTTTCT TTCGCCTTAA 11136 .......... .......... .......... .......... .......... .......... 428 TCTTCATCCT CTTTCTTTTC TTGTAACTGA GGGTGAGGTG ATGCATGGGT TTTCGAAAAT 11196 .......... .......... .......... .......... .......... .......... 428 TCTTTCGTTC ATTATTTCAT CTTCACTTTA GGGAGTGGGC TGAATATAAA ATGGGCTGAA 11256 .......... .......... .......... .......... .......... .......... 428 TATATAATAC TACAAAAATA TTGTATTAGG CCTGATGAAA TATAGTGGTA ATATAACTTA 11316 .......... .......... .......... .......... .......... .......... 428 CCTTTCGCGG TGGAACAGGC CAATCTTACA TTCCATGTGT GTGTGTATTG GAGCGGACCA 11376 || | .......... .......... .......... .......... .......... ......ACAA 432 ATCCATATTA CAAAGCATAT TAATGAATAA AAAACTGAGG TGAAATGATT ATTTCCATTT 11436 | ||||||| | || || || ACTCATATTA GAGAGGATCC TA........ .......... .......... .......... 454 TTATTTAGAA TCCCCTTAGC TGTGTTTGAT ATTCTATTTG TACCCACATA TCCTAGACAG 11496 || .......... .......... .......... .......... .......... ......CCA- 457 AATTTAATGA GTCTGAGTGT CTGACACTTC CGACACGGAT CATACCCCCC GATTTTAGAA 11556 ||||| | | AATTTGTTCA .......... .......... .......... .......... .......... 467 GAGTCTGTGT AATGTAGATC TTCCGTGTTT TAAAATTTAT CCCCAGGAGA TGTGTTAATA 11616 .......... .......... .......... .......... .......... .......... 467 ATTTATTTTG CAGATGCTTG AAGATTCTTT AGAAGAACGG TTAGAAAAGA CTGGAGGTTC 11676 .......... .......... .......... .......... .......... .......... 467 TGTTCCAGAT TCGGAAAAGT TTGATGGAGA TCCGAAGGAA GGAGAAGTGG AAGAAAACAA 11736 .......... .......... .......... .......... .......... .......... 467 GGTATTGAAT TTCTATCAGT TCATTTCCCA CTTGATCATA TTCATAAATA TTTCGCACTT 11796 .......... .......... .......... .......... .......... .......... 467 AATTATCTCA TCTTTCCTTA GTGGTGTAAA TTTTTTTTGG TGGACATCCT TCCACATTTA 11856 .......... .......... .......... .......... .......... .......... 467 CTTTTGTCGA AAAAGTGACA TCCTTCCACA TTTATTGTTT TTATTTTGCC CTTTTTTCAG 11916 .......... .......... .......... .......... .......... .......... 467 AAGAAGTCAA TTGAAGGTGG TAAACATGGT GCACCTCTTA AAAAACTAAA GAAGGACAAA 11976 .......... .......... .......... .......... .......... .......... 467 GTTGGGATTG CAACAGGGAA ATCTTCTCGT ACAGAGAGCA GCACAGTGAC AACTGCACCA 12036 .......... .......... .......... .......... .......... .......... 467 CGTGTCAAAC CGGAACTTGC TTTACCTGCT ATGTACTCCT CAAACCTATT ATCTTACTTA 12096 .......... .......... .......... .......... .......... .......... 467 CCAAAAGAAA AAGAGAGGGC CCTATTGTGT TTTTCTCTCC CACGACATCA TCCCTGTACT 12156 .......... .......... .......... .......... .......... .......... 467 TGGAAAAGTC ATTCTTGTTG GATGTCATAA TTTTATTTGA AATACAGTCA TCATGATCCA 12216 || || .......... .......... .......... .......... .......TCC TC........ 472 TTAGAAAACA TCAAGTGAAA CGGCTATACA AAGAAGTTGC ATAAAAAAGG AAAATTATGA 12276 .......... .......... .......... .......... .......... .......... 472 AGGATGAGGA AAATATGATT GAGCTCAATC CTTTCTGTGG TGTCATACTC ATAGTACTCC 12336 .......... .......... .......... .......... .......... .......... 472 CTCCATTTCA ATTTATTTGA CCTTTTTTCC TCTCTAGTCC ATTGAAAAAA GAATGACCCT 12396 .......... .......... .......... .......... .......... .......... 472 TTCCTTTTTA TAGCAACTTC TAAATTTTAC TTCCCAAGTT GTATGTTTAA GACTACAAGA 12456 .......... .......... .......... .......... .......... .......... 472 ATAAAGGGCA TTTCGGTACA TTTGACAGAT CTTTGATTTT AAACCACAAG ATTTAAAGAT 12516 .......... .......... .......... .......... .......... .......... 472 CTTTATTTTA TTAAACTCTG TGCCAAGTCA AAACAAGAAT TTGAAATGGA GGGAGTGTAT 12576 .......... .......... .......... .......... .......... .......... 472 TTTTTTAGAT AATTAAATAT CTTTAATAAA GTCATACTCG GTATATCTTC TTACATTCAT 12636 .......... .......... .......... .......... .......... .......... 472 CCATATATTG CATTATGTTT CTTATCTCTC TGTGTGTGTG GGGGGGGGGG GGTTGGATCT 12696 .......... .......... .......... .......... .......... .......... 472 TGGATCATCT TAACTGAAAG AAAATTTGTC ACAGACCTGT AGAGGTAGAA CTTTCTATTC 12756 .......... .......... .......... .......... .......... .......... 472 TGGAAGACTT GAGGAACCGC GTACAGTTGA GTAATGCATC ATTGCCCTCT GTTAGCTTCT 12816 .......... .......... .......... .......... .......... .......... 472 ATACATTTGT TAATACACAC AATGGGTAAG TTTTCATGAT CTTGAATCTT TTAAGTGTGG 12876 .......... .......... .......... .......... .......... .......... 472 AATTCGTATT CAGGAACATA TCCTAATCTC AGTGAGTTAA TCTGTAAATG TCTGTAGACT 12936 .......... .......... .......... .......... .......... .......... 472 AAACTGTGCA TCAATATCAC ATGATGGATC ACTAGTGGCT GGTGGATTCT CAGATTCATC 12996 .......... .......... .......... .......... .......... .......... 472 TGTGAAGGTG CTTGTAGCTT CTGAATGACG TTGAAATTAT GTTGTCACAA ATTACAGTAT 13056 .......... .......... .......... .......... .......... .......... 472 CTGTTGATCC TTGATCATCT TCTTATCTTT TTTTTGGAAC TGGTAACTTT GATCATCTTC 13116 .......... .......... .......... .......... .......... .......... 472 TAATCTAAGT CCCCCATTTT ACAGGTCTGG GACATGGCAA AGCTTGGGGA ACAAACTGGT 13176 .......... .......... .......... .......... .......... .......... 472 CCATGTAAGT CCAATATGAT GGTCCTCAGC TCCTTTTCAA TGTATTGTTA TTTCATGTTT 13236 .......... .......... .......... .......... .......... .......... 472 CAAGATGTTT TAGTGAATAG AGGTTGAAAA GGTCCCTTTT AATACTACTC CTAGTCACAG 13296 .......... .......... .......... .......... .......... .......... 472 TATATCTCGC CAGGACAGCA AGAAAGATTT ATTCTTTGCT GCTTTAATTT ATTTGATAAG 13356 .......... .......... .......... .......... .......... .......... 472 TAGATAAATT TTATTTCACT AAGAAACTGC ACTAGGGTTG TGAAGTTTGT TACATCAGAA 13416 .......... .......... .......... .......... .......... .......... 472 ATTTGCTTAG CAGAAACATG ATTAGTTATG AAGGTTTCTC GACTGAACAT ATAAATGCAG 13476 .......... .......... .......... .......... .......... .......... 472 CTTTACATTT CTGATGGAAG TATTTCTTGA ACTCCTTGCA TTTTTCAATT AGGTAAATAA 13536 .......... .......... .......... .......... .......... .......... 472 TTTTAATCGA AAAGTCTCCT GTATACAATG CAGTACACAA AAAATAACTA TATCAGAATA 13596 ||| ||| || | ||| ||| | .......... .......... .......... ...TACTCAA TGAAGACTGC TATGTGAAGA 499 -TGATTCTAA CAAACAATAT CCTATTGTTT TTTTTTTGGA GAAAGTATCA GAATATGATT 13655 | ||||| | ||| | CTTATTCT-A GAAAGA.... .......... .......... .......... .......... 514 CTAACAAACA ATATCCTACT GTTTTTTGTT TTTTTTGAGA AAGTAACATT TGTATTAAAG 13715 .......... .......... .......... .......... .......... .......... 514 AAACTTGGTA AAAAGGTTGT GCTGGTAACC ATGTGCAGCT TCCAAAAGTA TTGGGAATCC 13775 .......... .......... .......... .......... .......... .......... 514 TTTTACTTCT CACTAGGGAT CTATCATGTC ATATTGGATT CTACATCATT TACATAATCT 13835 .......... .......... .......... .......... .......... .......... 514 CTTTGCACCA AAAACATAAA CAACACAAAA CAATTCATCT CTATCATTTG AATGAGGCTA 13895 .......... .......... .......... .......... .......... .......... 514 TTGCTGCCTT CAAAACATCT AGGGTTCCTT TCCTTCAAAG CTGTCAACCA AATGCTGGAG 13955 |||| | ||| ||||| .......... .......... .......... .......... ...TCAATCC AATACTGGA. 530 TGAGATCTCC CTGATTGTGC CAACATCTCA GTAAATCAGC TGTAGTTCTT GGCACTGTCC 14015 .......... .......... .......... .......... .......... .......... 530 ATCCGAGTCC AACTATGCTT GGGAAGAGTT GCTACAATTG AGCAGTTACA CCAAGATGTA 14075 .......... .......... .......... .......... .......... .......... 530 AGAACAGGTG TCTGTTAGAC TCTTTAAGTC TGCACAGTAT TACTAACATC TGGTGCTCAA 14135 .......... .......... .......... .......... .......... .......... 530 CTGGAATCCT CTCTTCTTTA AATTTATCGA GTTAGACTGG CCTATAAGAA CTTCACTAAT 14195 .......... .......... .......... .......... .......... .......... 530 GCACCAAAAA GAATCAAGGG ATTGAAAAGC TATTCCTTGA GTCTACATAC TCGTTTTCTA 14255 .......... .......... .......... .......... .......... .......... 530 TCTCTTAAAA AGTCTTGAAC CACTCCTATA ATGTTGAAGT GATCAATAAT TGAGAATTTT 14315 .......... .......... .......... .......... .......... .......... 530 GTCACATGTC TTGTTATGAC GCTCATCATG TATGCTAGTG GTTTAGATAC TCCTAAGGAA 14375 .......... .......... .......... .......... .......... .......... 530 AAGGCAAACA TTTTGAATCC AAATGTTAAC ATGAGGTTTT TTTTTGAGGA TAGTAACACT 14435 .......... .......... .......... .......... .......... .......... 530 AATCTAAATA TTCACAGCTA GACTACATCC AAAATATGTA GTCCCCTTCA AAAGGTATTG 14495 .......... .......... .......... .......... .......... .......... 530 CAGTCTAGTA ATATTGCCGT CTATCACCTA TATTTCTTAC AAATAATCCA AGACATCCCA 14555 .......... .......... .......... .......... .......... .......... 530 GATATCTTGC GCTTGGACTA AAAAGTTCTC TTTTCACCAA AAATAAAATA AAGCTAGAGA 14615 .......... .......... .......... .......... .......... .......... 530 ATTCATCTTG AAGCTCTGAA GTCCATTCTG CTTGTCTTCA AAGCACCTCT GGTTAGAGTT 14675 .......... .......... .......... .......... .......... .......... 530 TTCTCAAAAG GCCTGCATAT CCACTATGGT TCAGGGCCGA TCATAATCCC TATGATGTGA 14735 .......... .......... .......... .......... .......... .......... 530 TAATTCCGTT GAATAAGGTG GAATGTAAGT AAAAGAAATT CATTTATAAA TACCAATTAA 14795 .......... .......... .......... .......... .......... .......... 530 GTGCCAAACA ATGCGTAGAT GTCCATGTTA CTTGGAAAAG TTTCCATATG ATGCTATATT 14855 .......... .......... .......... .......... .......... .......... 530 TCATGTTCTT TATTCATTCT TTGTTATTTC GTATTAATTA TTTACTTCTT ATTACAGCCT 14915 .......... .......... .......... .......... .......... .......... 530 TTTATATAGT TGAGCTTGTG CTAAGTTGTT TATTCTTTTG CACCATCAGT AATATTCTGA 14975 .......... .......... .......... .......... .......... .......... 530 ATGTTGCATG TGATGTATCA GGTTGAGGGT AGTTTTGCTA TAAAAATTGA TTCCACCTTT 15035 ||| .......... .......... .AGAGAGC.. .......... .......... .......... 537 ATCTTGTCCT AAATTCTAAG TTGGATTTTC TTTTCTGATA CTCTCACTAC AATCCCTCAG 15095 .......... .......... .......... .......... .......... .......... 537 GAAAATCTAG TTATATTGTA AAACTGGCAT ATTTGCCTTG AGCTGTCATA TTCTAACTTG 15155 .......... .......... .......... .......... .......... .......... 537 CAGAAAGGAA ATAAGACAAA TAGCTCATAG TTCCTTACAA ATGCACGCCT TTGGAGCCAG 15215 .......... .......... .......... .......... .......... .......... 537 CTGTTATGTT ATTCTCTAAG GCATATCAGC AGTCAAGATT GATTCTTAAA CTGATTTTTC 15275 .......... .......... .......... .......... .......... .......... 537 AGCTCCATGA ATTTGGTCTA TTGGTAAGAG CACATCATGT AATGTTTGAG TTAGAGGCAT 15335 .......... .......... .......... .......... .......... .......... 537 GTCACGAGTT TGAACCCAAC CACTTCAAAA AACATAGTTT TTAAGTATAG AATGTGAGAG 15395 .......... .......... .......... .......... .......... .......... 537 GGGCGACTCC AATATCCACC GAGTTTTGGA CTGCGTCGGC TAGCCCTCGG GGATTTCTGG 15455 .......... .......... .......... .......... .......... .......... 537 GTTATCAATT TTCTTAAAAA AGCTGCTATC TCCATACTAA TTGCTTATTG AGGGTTATCA 15515 .......... .......... .......... .......... .......... .......... 537 ATTTTCTTAT AAAAGCTGCT ATCTCCATAC AAATTGCTTA TTGAGGATGA ATTTGAGCAG 15575 .......... .......... .......... .......... .......... .......... 537 TTACTGAAGC AACTAATTTT GAATAATTTG TCCTTCAAAA ATAAAACCCA CATATCAAGA 15635 .......... .......... .......... .......... .......... .......... 537 ACTAAATGAA AGGTTATTGC TTCAGGGGTG TAGTTGTTCT TTAATATCTA TAAAAGTATG 15695 .......... .......... .......... .......... .......... .......... 537 TGAAAGCAAT TTAACCAAGG ATTAGTTTGT TTGACTCGCT GAAGCAAAAG AAGGCTACTA 15755 .......... .......... .......... .......... .......... .......... 537 CTTTGGGGCC AGGGTAGCCA TAGATAGTCT GACGTCTTAG AGTATTGCTG TAAACAATCA 15815 .......... .......... .......... .......... .......... .......... 537 ACATCTTGCA AAACATGTCA TTGTTAATGT GTGCCCCCCT GTCTCTCATA CACACAAAAA 15875 .......... .......... .......... .......... .......... .......... 537 ATTGCCGAAA CAGAAAAAAG AATGGTTGAT GAACAGTGCT AGAACCAGCT TTTTTGCAGG 15935 .......... .......... .......... .......... .......... .......... 537 GGGAGACTGA TTCACCTTCA AGTGAGCATG TGCTTGGCGC AAATGGTGGA AGAAGATGTT 15995 .......... .......... .......... .......... .......... .......... 537 ATACATTACT TCAGGGCCAT TCAGGGCCTG TTTATTCTGC ATCATTCAGT CCGCACGGGG 16055 .......... .......... .......... .......... .......... .......... 537 ATTTTCTTCT TTCTTCATCA TCAGATTCAA CTGGTATGAT ATCATATGAC TTTGATTGCT 16115 .......... .......... .......... .......... .......... .......... 537 ATTAGCTCTA CAATAGTTCT TATTCATATG GATTATGATA GTTACTGAAG TTCTATATGC 16175 .......... .......... .......... .......... .......... .......... 537 TTACTCTATA ACCATGTGAC CTTTTTGGCT AATTATTTCT ATGCAGTTCG CTTATGGAGC 16235 .......... .......... .......... .......... .......... .......... 537 ACAAAGTTAA ATGCTAATGT TGTTTGCTAC AAAGGCCACA ATTACCCAGT ATGGGATGTT 16295 .......... .......... .......... .......... .......... .......... 537 CAGGTAGGTT GCTAGTTTGT CATTTATTTT GTTTAATGAT TCCTTTTTGG ATGTAATTGT 16355 .......... .......... .......... .......... .......... .......... 537 GGTAATATAT TTGCAATCTC TTTAAGTAAC TCACCTAATT GGTTGTTAAA CAGAGAACTG 16415 .......... .......... .......... .......... .......... .......... 537 CCCGAGTTAA TGCATTTTTT GCTTTCTCAT GGTAACTGTA GATAAAAGTT GTCACAATGG 16475 .......... .......... .......... .......... .......... .......... 537 GCAAATATAT GTTTGGTTTT CACTTCACAT CTTGAGTGCA GTTTAGCCCA GCTGGACATT 16535 .......... .......... .......... .......... .......... .......... 537 ATTTTGCCAG TTCTTCACAT GACCGCACTG CAAGGATCTG GTCTGTGGAC AGGATACAGC 16595 .......... .......... .......... .......... .......... .......... 537 CTCTAAGAAT AATGGCCGGA CACTTGTCAG ATGTAGAT 16633 | | | ||| ||||||| .......... .......... .AATGGCCAG TTGTAGAT 554 hqPGS_C06HBa0046G10.1-4+_SGN-E348402+ (10477 10670) ******************************************************************************** EST sequence 14 +strand 723 n (File: SGN-E332440+) 1 GCAAACACTG ATATTTACAT GTTTGGGAAG CTCCACGGAG GACTTTAAGT GTTAGCTTAT 61 TGAAAAGAAA AATTAAGAAA CAAAAGAAAA AGAAAATACC TCGAATGTGA AATTTGGAGT 121 AACTTTTGTA TCGTAGAAGC TATTTTTTTA AGAAAGAGTA TACTGTTTAG TACATGATGT 181 GGTGTTGGAA TAATTCAAGT GCACTTGAAT TGAGTTGGGC AAAAGCTAGA GTTGTGTCCT 241 TTAGGGTGGC CCAGTGGTTT GGGTATGGGA CTTCCATGTT GGAGGTTTCA AGTTTGAAAC 301 CCCTTGCCAG CGAAAGCAAG GGGTTTGCCT TCTGGGTCGA GCTCATCGCA TCAGGCTTGC 361 CTAGTGTGGG GTACCTCTCT TGTGTTGTTT GCGAGCAATT GCATAGGAGC GGGGTTTTTT 421 TCTGTGTGCA CCCAAAGGGT AGCAGCTGCT GGTTTCCCTT GTCATTAAAA GAAAAAAGAG 481 CTACCAGAGC TGTAACGGTC TTTTTAAGAT GAGAAATCAA TAATAATCCT TGTAAAAAGT 541 TTTCAGCAAA TATTGGACTT ATTAACTGAA TTGTTTTCTT TAAGTGCTTA CTGAAGCTCT 601 AGGACAATAT CATTAATTTC AGGTCAATGC TGCTGGGGAT CTCTGTGCTA TTTGCCAGGA 661 GAAAATGCAT ACACCAATCT TACTACGTTG TAAACATATA TTCTGTGAAG ACTGTGTGTC 721 TGA Predicted gene structure (within gDNA segment 7456 to 14696): Exon 1 10479 10724 ( 246 n); cDNA 237 480 ( 244 n); score: 0.878 Intron 1 10725 13753 (3029 n); Pd: 0.762 (s: 0.84), Pa: 0.821 (s: 0) Exon 2 13754 13759 ( 6 n); cDNA 481 486 ( 6 n); score: 0.833 MATCH C06HBa0046G10.1-4+ SGN-E332440+ 0.878 252 0.349 C PGS_C06HBa0046G10.1-4+_SGN-E332440+ (10479 10724,13754 13759) Alignment (genomic DNA sequence = upper lines): TCCTTTGGGG TGGCCCAGTG GTTTGAGTTT GTGACTTCCA TGTTGGAGGT CTCAAGTTCG 10538 |||||| ||| |||||||||| ||||| || | | |||||||| |||||||||| ||||||| | TCCTTTAGGG TGGCCCAGTG GTTTGGGTAT GGGACTTCCA TGTTGGAGGT TTCAAGTTTG 296 AAACCCCTTG CCAGCGAAAG CAAGGAGTTT GCCTTCTGGG TCGAGCTCGT CGCACCAGGT 10598 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||| | |||| |||| AAACCCCTTG CCAGCGAAAG CAAGGGGTTT GCCTTCTGGG TCGAGCTCAT CGCATCAGGC 356 TTGTCTAGTG CGTGTTACCT CTCATATGTG GTTTGCGAGC TATTGCATAG GAGCGGGGTT 10658 ||| |||||| | | ||||| ||| | ||| |||||||||| ||||||||| |||||||||| TTGCCTAGTG TGGGGTACCT CTCTTGTGTT GTTTGCGAGC AATTGCATAG GAGCGGGGTT 416 TTTACCCTGT GCGCACCCAA AGGGTAGCGG CTGTGGGTTT CCCTTGTCAT AAAAAAAGAA 10718 ||| |||| | |||||||| |||||||| | ||| ||||| |||||||||| |||| | || TTT-TTCTGT GTGCACCCAA AGGGTAGCAG CTGCTGGTTT CCCTTGTCAT TAAAAGA-AA 474 GAAGAAGTAT ATCACTATCA ACTAGTTTTA GCCATTCTAG GTTCCATGTT TTGTAAGGAT 10778 |||| AAAGAG.... .......... .......... .......... .......... .......... 480 TGCTTGGGTT ATGCTCTTGA TCACACTTCC TCAATGTGGG TGGAATACAT GTTGGATTTG 10838 .......... .......... .......... .......... .......... .......... 480 ATTAAAATAA GATGGGTAAT TAAATGGGTG CAAACCTAGG AGACAAGTCA AAAATACTAG 10898 .......... .......... .......... .......... .......... .......... 480 GTGATTTCTT CTCATCTGCC CAAGCTTGGT AGTTGATTTA TCCTGTAACT GTGTTGGTGG 10958 .......... .......... .......... .......... .......... .......... 480 GAGAAAGCAA GTACCTAACG GAATACTCAA GGTGCACACA AGTTGTCATA AAACACCACC 11018 .......... .......... .......... .......... .......... .......... 480 ATTTTCAAGT TAAAAAAAAT GTATTATTTG ACTTTCATAT GGCGAATGAA GAGGTCAGAG 11078 .......... .......... .......... .......... .......... .......... 480 AGACTAGAGT CAAAAGATAA AGAATGTCGT GATGGAGTTG TCCTTTCTTT CGCCTTAATC 11138 .......... .......... .......... .......... .......... .......... 480 TTCATCCTCT TTCTTTTCTT GTAACTGAGG GTGAGGTGAT GCATGGGTTT TCGAAAATTC 11198 .......... .......... .......... .......... .......... .......... 480 TTTCGTTCAT TATTTCATCT TCACTTTAGG GAGTGGGCTG AATATAAAAT GGGCTGAATA 11258 .......... .......... .......... .......... .......... .......... 480 TATAATACTA CAAAAATATT GTATTAGGCC TGATGAAATA TAGTGGTAAT ATAACTTACC 11318 .......... .......... .......... .......... .......... .......... 480 TTTCGCGGTG GAACAGGCCA ATCTTACATT CCATGTGTGT GTGTATTGGA GCGGACCAAT 11378 .......... .......... .......... .......... .......... .......... 480 CCATATTACA AAGCATATTA ATGAATAAAA AACTGAGGTG AAATGATTAT TTCCATTTTT 11438 .......... .......... .......... .......... .......... .......... 480 ATTTAGAATC CCCTTAGCTG TGTTTGATAT TCTATTTGTA CCCACATATC CTAGACAGAA 11498 .......... .......... .......... .......... .......... .......... 480 TTTAATGAGT CTGAGTGTCT GACACTTCCG ACACGGATCA TACCCCCCGA TTTTAGAAGA 11558 .......... .......... .......... .......... .......... .......... 480 GTCTGTGTAA TGTAGATCTT CCGTGTTTTA AAATTTATCC CCAGGAGATG TGTTAATAAT 11618 .......... .......... .......... .......... .......... .......... 480 TTATTTTGCA GATGCTTGAA GATTCTTTAG AAGAACGGTT AGAAAAGACT GGAGGTTCTG 11678 .......... .......... .......... .......... .......... .......... 480 TTCCAGATTC GGAAAAGTTT GATGGAGATC CGAAGGAAGG AGAAGTGGAA GAAAACAAGG 11738 .......... .......... .......... .......... .......... .......... 480 TATTGAATTT CTATCAGTTC ATTTCCCACT TGATCATATT CATAAATATT TCGCACTTAA 11798 .......... .......... .......... .......... .......... .......... 480 TTATCTCATC TTTCCTTAGT GGTGTAAATT TTTTTTGGTG GACATCCTTC CACATTTACT 11858 .......... .......... .......... .......... .......... .......... 480 TTTGTCGAAA AAGTGACATC CTTCCACATT TATTGTTTTT ATTTTGCCCT TTTTTCAGAA 11918 .......... .......... .......... .......... .......... .......... 480 GAAGTCAATT GAAGGTGGTA AACATGGTGC ACCTCTTAAA AAACTAAAGA AGGACAAAGT 11978 .......... .......... .......... .......... .......... .......... 480 TGGGATTGCA ACAGGGAAAT CTTCTCGTAC AGAGAGCAGC ACAGTGACAA CTGCACCACG 12038 .......... .......... .......... .......... .......... .......... 480 TGTCAAACCG GAACTTGCTT TACCTGCTAT GTACTCCTCA AACCTATTAT CTTACTTACC 12098 .......... .......... .......... .......... .......... .......... 480 AAAAGAAAAA GAGAGGGCCC TATTGTGTTT TTCTCTCCCA CGACATCATC CCTGTACTTG 12158 .......... .......... .......... .......... .......... .......... 480 GAAAAGTCAT TCTTGTTGGA TGTCATAATT TTATTTGAAA TACAGTCATC ATGATCCATT 12218 .......... .......... .......... .......... .......... .......... 480 AGAAAACATC AAGTGAAACG GCTATACAAA GAAGTTGCAT AAAAAAGGAA AATTATGAAG 12278 .......... .......... .......... .......... .......... .......... 480 GATGAGGAAA ATATGATTGA GCTCAATCCT TTCTGTGGTG TCATACTCAT AGTACTCCCT 12338 .......... .......... .......... .......... .......... .......... 480 CCATTTCAAT TTATTTGACC TTTTTTCCTC TCTAGTCCAT TGAAAAAAGA ATGACCCTTT 12398 .......... .......... .......... .......... .......... .......... 480 CCTTTTTATA GCAACTTCTA AATTTTACTT CCCAAGTTGT ATGTTTAAGA CTACAAGAAT 12458 .......... .......... .......... .......... .......... .......... 480 AAAGGGCATT TCGGTACATT TGACAGATCT TTGATTTTAA ACCACAAGAT TTAAAGATCT 12518 .......... .......... .......... .......... .......... .......... 480 TTATTTTATT AAACTCTGTG CCAAGTCAAA ACAAGAATTT GAAATGGAGG GAGTGTATTT 12578 .......... .......... .......... .......... .......... .......... 480 TTTTAGATAA TTAAATATCT TTAATAAAGT CATACTCGGT ATATCTTCTT ACATTCATCC 12638 .......... .......... .......... .......... .......... .......... 480 ATATATTGCA TTATGTTTCT TATCTCTCTG TGTGTGTGGG GGGGGGGGGG TTGGATCTTG 12698 .......... .......... .......... .......... .......... .......... 480 GATCATCTTA ACTGAAAGAA AATTTGTCAC AGACCTGTAG AGGTAGAACT TTCTATTCTG 12758 .......... .......... .......... .......... .......... .......... 480 GAAGACTTGA GGAACCGCGT ACAGTTGAGT AATGCATCAT TGCCCTCTGT TAGCTTCTAT 12818 .......... .......... .......... .......... .......... .......... 480 ACATTTGTTA ATACACACAA TGGGTAAGTT TTCATGATCT TGAATCTTTT AAGTGTGGAA 12878 .......... .......... .......... .......... .......... .......... 480 TTCGTATTCA GGAACATATC CTAATCTCAG TGAGTTAATC TGTAAATGTC TGTAGACTAA 12938 .......... .......... .......... .......... .......... .......... 480 ACTGTGCATC AATATCACAT GATGGATCAC TAGTGGCTGG TGGATTCTCA GATTCATCTG 12998 .......... .......... .......... .......... .......... .......... 480 TGAAGGTGCT TGTAGCTTCT GAATGACGTT GAAATTATGT TGTCACAAAT TACAGTATCT 13058 .......... .......... .......... .......... .......... .......... 480 GTTGATCCTT GATCATCTTC TTATCTTTTT TTTGGAACTG GTAACTTTGA TCATCTTCTA 13118 .......... .......... .......... .......... .......... .......... 480 ATCTAAGTCC CCCATTTTAC AGGTCTGGGA CATGGCAAAG CTTGGGGAAC AAACTGGTCC 13178 .......... .......... .......... .......... .......... .......... 480 ATGTAAGTCC AATATGATGG TCCTCAGCTC CTTTTCAATG TATTGTTATT TCATGTTTCA 13238 .......... .......... .......... .......... .......... .......... 480 AGATGTTTTA GTGAATAGAG GTTGAAAAGG TCCCTTTTAA TACTACTCCT AGTCACAGTA 13298 .......... .......... .......... .......... .......... .......... 480 TATCTCGCCA GGACAGCAAG AAAGATTTAT TCTTTGCTGC TTTAATTTAT TTGATAAGTA 13358 .......... .......... .......... .......... .......... .......... 480 GATAAATTTT ATTTCACTAA GAAACTGCAC TAGGGTTGTG AAGTTTGTTA CATCAGAAAT 13418 .......... .......... .......... .......... .......... .......... 480 TTGCTTAGCA GAAACATGAT TAGTTATGAA GGTTTCTCGA CTGAACATAT AAATGCAGCT 13478 .......... .......... .......... .......... .......... .......... 480 TTACATTTCT GATGGAAGTA TTTCTTGAAC TCCTTGCATT TTTCAATTAG GTAAATAATT 13538 .......... .......... .......... .......... .......... .......... 480 TTAATCGAAA AGTCTCCTGT ATACAATGCA GTACACAAAA AATAACTATA TCAGAATATG 13598 .......... .......... .......... .......... .......... .......... 480 ATTCTAACAA ACAATATCCT ATTGTTTTTT TTTTGGAGAA AGTATCAGAA TATGATTCTA 13658 .......... .......... .......... .......... .......... .......... 480 ACAAACAATA TCCTACTGTT TTTTGTTTTT TTTGAGAAAG TAACATTTGT ATTAAAGAAA 13718 .......... .......... .......... .......... .......... .......... 480 CTTGGTAAAA AGGTTGTGCT GGTAACCATG TGCAGCTTCC A 13759 || || | .......... .......... .......... .....CTACC A 486 hqPGS_C06HBa0046G10.1-4+_SGN-E332440+ (10479 10724) ******************************************************************************** EST sequence 22 -strand 420 n (File: SGN-E220828-) 1 CATGTTACGT ATCTCTGCAA ATTCCCCATC CTAATAAAAC AGCCTATGTT GCTCGAACTC 61 TCCAAAAACA CCACTGCACC TCTGTCGGAT CCTCTTAACA ATATACTCAG TGTAATTCCA 121 TACTAATAAG TGATATATAT ATATATATAT ATATATACAT ACACACACAC ACACCCTTCG 181 GGGTGGCCTA GTGGTTTGAG CTTGGGACTT CCATGTTGGA GGTCTCAAGT TCGAAACCCC 241 TTGCCAGTAA AAGCAAGGGG TTTGCCTTCT GGGTCGAGCT CGTCGCACCA GGCTTGCCTA 301 GTGCGGGTTA CCTCTTCTAT GTGGTTTGCG AGCTATTGTA TAGGAGCGAG GGATATACCC 361 TGTGTGCATC CAAAGGGTAG CGTCTGCGAG TTTCCCTTGT CATAAAAAAA AAAAAAAAAA Predicted gene structure (within gDNA segment 7851 to 12477): Exon 1 10480 10724 ( 245 n); cDNA 175 419 ( 245 n); score: 0.898 MATCH C06HBa0046G10.1-4+ SGN-E220828- 0.898 245 0.583 C PGS_C06HBa0046G10.1-4+_SGN-E220828- (10480 10724) Alignment (genomic DNA sequence = upper lines): CCTTTGGGGT GGCCCAGTGG TTTGAGTTTG TGACTTCCAT GTTGGAGGTC TCAAGTTCGA 10539 |||| ||||| |||| ||||| |||||| ||| ||||||||| |||||||||| |||||||||| CCTTCGGGGT GGCCTAGTGG TTTGAGCTTG GGACTTCCAT GTTGGAGGTC TCAAGTTCGA 234 AACCCCTTGC CAGCGAAAGC AAGGAGTTTG CCTTCTGGGT CGAGCTCGTC GCACCAGGTT 10599 |||||||||| ||| ||||| |||| ||||| |||||||||| |||||||||| |||||||| | AACCCCTTGC CAGTAAAAGC AAGGGGTTTG CCTTCTGGGT CGAGCTCGTC GCACCAGGCT 294 TGTCTAGTGC GTGTTACCTC TCATATGTGG TTTGCGAGCT ATTGCATAGG AGCGGGGTTT 10659 || ||||||| | |||||||| | ||||||| |||||||||| |||| ||||| |||| || | TGCCTAGTGC GGGTTACCTC TTCTATGTGG TTTGCGAGCT ATTGTATAGG AGCGAGGGAT 354 TTACCCTGTG CGCACCCAAA GGGTAGCGGC TGTGGGTTTC CCTTGTCATA AAAAAAGAAG 10719 ||||||||| ||| ||||| |||||||| | || | ||||| |||||||||| |||||| || ATACCCTGTG TGCATCCAAA GGGTAGCGTC TGCGAGTTTC CCTTGTCATA AAAAAAAAAA 414 AAGAA 10724 || || AAAAA 419 hqPGS_C06HBa0046G10.1-4+_SGN-E220828- (10480 10724) ******************************************************************************** EST sequence 23 -strand 475 n (File: SGN-E220902-) 1 AATAATTCCG TCTAGTCCTA TTTCTATTTG TCCCACAATA CCTTAATTTA TATATCATGT 61 TACGTATCTC TGCAAATTCC CCATCCTAAT AAAACAGCCT ATGTTGCTCG AACTCTCCAA 121 AAACACCACT GCACCTCTGT CGGATCCTCT TAACAATATA CTCAGTGTAA TTCCATACTA 181 ATAAGTGATA TATATATATA TATATATATA TACATACACA CACACACACC CTTCGGGGTG 241 GCCTAGTGGT TTGAGCTTGG GACTTCCATG TTGGAGGTCT CAAGTTCGAA ACCCCTTGCC 301 AGTAAAAGCA AGGGGTTTGC CTTCTGGGTC GAGCTCGTCG CACCAGGCTT GCCTAGTGCG 361 GGTTACCTCT TCTATGTGGT TTGCGAGCTA TTGTATAGGA GCGAGGGATA TACCCTGTGT 421 GCATCCAAAG GGTAGCGTCT GCGAGTTTCC CTTGTCATAA AAAAAAAAAA AAAAA Predicted gene structure (within gDNA segment 7301 to 12477): Exon 1 10480 10724 ( 245 n); cDNA 230 474 ( 245 n); score: 0.898 MATCH C06HBa0046G10.1-4+ SGN-E220902- 0.898 245 0.516 C PGS_C06HBa0046G10.1-4+_SGN-E220902- (10480 10724) Alignment (genomic DNA sequence = upper lines): CCTTTGGGGT GGCCCAGTGG TTTGAGTTTG TGACTTCCAT GTTGGAGGTC TCAAGTTCGA 10539 |||| ||||| |||| ||||| |||||| ||| ||||||||| |||||||||| |||||||||| CCTTCGGGGT GGCCTAGTGG TTTGAGCTTG GGACTTCCAT GTTGGAGGTC TCAAGTTCGA 289 AACCCCTTGC CAGCGAAAGC AAGGAGTTTG CCTTCTGGGT CGAGCTCGTC GCACCAGGTT 10599 |||||||||| ||| ||||| |||| ||||| |||||||||| |||||||||| |||||||| | AACCCCTTGC CAGTAAAAGC AAGGGGTTTG CCTTCTGGGT CGAGCTCGTC GCACCAGGCT 349 TGTCTAGTGC GTGTTACCTC TCATATGTGG TTTGCGAGCT ATTGCATAGG AGCGGGGTTT 10659 || ||||||| | |||||||| | ||||||| |||||||||| |||| ||||| |||| || | TGCCTAGTGC GGGTTACCTC TTCTATGTGG TTTGCGAGCT ATTGTATAGG AGCGAGGGAT 409 TTACCCTGTG CGCACCCAAA GGGTAGCGGC TGTGGGTTTC CCTTGTCATA AAAAAAGAAG 10719 ||||||||| ||| ||||| |||||||| | || | ||||| |||||||||| |||||| || ATACCCTGTG TGCATCCAAA GGGTAGCGTC TGCGAGTTTC CCTTGTCATA AAAAAAAAAA 469 AAGAA 10724 || || AAAAA 474 hqPGS_C06HBa0046G10.1-4+_SGN-E220902- (10480 10724) ******************************************************************************** EST sequence 2 +strand 824 n (File: SGN-E262068+) 1 TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT TTGTTAATAC ACACAATGGA 61 CTAAACTGTG CATCAATATC ACATGATGGA TCACTAGTGG CTGGTGGATT CTCAGATTCA 121 TCTGTGAAGG TCTGGGACAT GGCAAAGCTT GGGGAACAAA CTGGTCCATC TTTTTTGCAG 181 GGGGAGACTG ATTCACCTTC AAGTGAGCAT GTGCTTGGCG CAAATGGTGG AAGAAGATGT 241 TATACATTAC TTCAGGGCCA TTCAGGGCCT GTTTATTCTG CATCATTCAG TCCTCACGGG 301 GATTTTCTTC TTTCTTCATC ATCAGATTCA ACTGTTCGCT TATGGAGCAC AAAGTTAAAT 361 GCTAATGTTG TTTGCTACAA AGGCCACAAT TACCCAGTAT GGGATGTTCA GTTTAGCCCA 421 GCTGGACATT ATTTTGCCAG TTCTTCACAT GACCGCACTG CAAGGATCTG GTCTGTGGAC 481 AGGATACAGC CTCTAAGAAT AATGGCCGGA CACTTGTCAG ATGTAGATTG TGTGCGATGG 541 CACGCAAATT GCAATTACAT TGCAACCGGA TCAAGCGACA AAACTGTACG CTTGTGGGAC 601 GTCCAGAGTG GTGACTGTGT CAGAATTTTC ATTGGTCATA GGAGTATGAT CCTATCATTG 661 GCAATGTCTC CTGATGGTCG ATATATGGCA TCCGGTGATG AAGATGGAAC AGTCATGATG 721 TGGGATCTCT CAAGTGGACG TTGTATTACT CCTTTGACCG GTCATTCCCT CTGTGTGTGG 781 TCACTTGCTT TTTAGTGGTG AAGGTCACTT CTTGCATCGG GCTC Predicted gene structure (within gDNA segment 12183 to 18878): Exon 1 12783 12841 ( 59 n); cDNA 1 59 ( 59 n); score: 1.000 Intron 1 12842 12933 ( 92 n); Pd: 0.980 (s: 1.00), Pa: 0.527 (s: 1.00) Exon 2 12934 13003 ( 70 n); cDNA 60 129 ( 70 n); score: 1.000 Intron 2 13004 13140 ( 137 n); Pd: 0.906 (s: 1.00), Pa: 0.980 (s: 1.00) Exon 3 13141 13180 ( 40 n); cDNA 130 169 ( 40 n); score: 1.000 Intron 3 13181 15923 (2743 n); Pd: 0.995 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 4 15924 16088 ( 165 n); cDNA 170 334 ( 165 n); score: 0.994 Intron 4 16089 16221 ( 133 n); Pd: 0.992 (s: 0.98), Pa: 0.952 (s: 1.00) Exon 5 16222 16298 ( 77 n); cDNA 335 411 ( 77 n); score: 1.000 Intron 5 16299 16516 ( 218 n); Pd: 0.978 (s: 1.00), Pa: 0.764 (s: 1.00) Exon 6 16517 16633 ( 117 n); cDNA 412 528 ( 117 n); score: 1.000 Intron 6 16634 17684 (1051 n); Pd: 0.880 (s: 1.00), Pa: 0.958 (s: 1.00) Exon 7 17685 17950 ( 266 n); cDNA 529 795 ( 267 n); score: 0.979 Intron 7 17951 18062 ( 112 n); Pd: 0.000 (s: 0.89), Pa: 0.990 (s: 0) Exon 8 18063 18092 ( 30 n); cDNA 796 824 ( 29 n); score: 0.967 MATCH C06HBa0046G10.1-4+ SGN-E262068+ 0.991 824 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E262068+ (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17950,18063 18092) Alignment (genomic DNA sequence = upper lines): TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT TTGTTAATAC ACACAATGGG 12842 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT TTGTTAATAC ACACAATGG. 59 TAAGTTTTCA TGATCTTGAA TCTTTTAAGT GTGGAATTCG TATTCAGGAA CATATCCTAA 12902 .......... .......... .......... .......... .......... .......... 59 TCTCAGTGAG TTAATCTGTA AATGTCTGTA GACTAAACTG TGCATCAATA TCACATGATG 12962 ||||||||| |||||||||| |||||||||| .......... .......... .......... .ACTAAACTG TGCATCAATA TCACATGATG 88 GATCACTAGT GGCTGGTGGA TTCTCAGATT CATCTGTGAA GGTGCTTGTA GCTTCTGAAT 13022 |||||||||| |||||||||| |||||||||| |||||||||| | GATCACTAGT GGCTGGTGGA TTCTCAGATT CATCTGTGAA G......... .......... 129 GACGTTGAAA TTATGTTGTC ACAAATTACA GTATCTGTTG ATCCTTGATC ATCTTCTTAT 13082 .......... .......... .......... .......... .......... .......... 129 CTTTTTTTTG GAACTGGTAA CTTTGATCAT CTTCTAATCT AAGTCCCCCA TTTTACAGGT 13142 || .......... .......... .......... .......... .......... ........GT 131 CTGGGACATG GCAAAGCTTG GGGAACAAAC TGGTCCATGT AAGTCCAATA TGATGGTCCT 13202 |||||||||| |||||||||| |||||||||| |||||||| CTGGGACATG GCAAAGCTTG GGGAACAAAC TGGTCCAT.. .......... .......... 169 CAGCTCCTTT TCAATGTATT GTTATTTCAT GTTTCAAGAT GTTTTAGTGA ATAGAGGTTG 13262 .......... .......... .......... .......... .......... .......... 169 AAAAGGTCCC TTTTAATACT ACTCCTAGTC ACAGTATATC TCGCCAGGAC AGCAAGAAAG 13322 .......... .......... .......... .......... .......... .......... 169 ATTTATTCTT TGCTGCTTTA ATTTATTTGA TAAGTAGATA AATTTTATTT CACTAAGAAA 13382 .......... .......... .......... .......... .......... .......... 169 CTGCACTAGG GTTGTGAAGT TTGTTACATC AGAAATTTGC TTAGCAGAAA CATGATTAGT 13442 .......... .......... .......... .......... .......... .......... 169 TATGAAGGTT TCTCGACTGA ACATATAAAT GCAGCTTTAC ATTTCTGATG GAAGTATTTC 13502 .......... .......... .......... .......... .......... .......... 169 TTGAACTCCT TGCATTTTTC AATTAGGTAA ATAATTTTAA TCGAAAAGTC TCCTGTATAC 13562 .......... .......... .......... .......... .......... .......... 169 AATGCAGTAC ACAAAAAATA ACTATATCAG AATATGATTC TAACAAACAA TATCCTATTG 13622 .......... .......... .......... .......... .......... .......... 169 TTTTTTTTTT GGAGAAAGTA TCAGAATATG ATTCTAACAA ACAATATCCT ACTGTTTTTT 13682 .......... .......... .......... .......... .......... .......... 169 GTTTTTTTTG AGAAAGTAAC ATTTGTATTA AAGAAACTTG GTAAAAAGGT TGTGCTGGTA 13742 .......... .......... .......... .......... .......... .......... 169 ACCATGTGCA GCTTCCAAAA GTATTGGGAA TCCTTTTACT TCTCACTAGG GATCTATCAT 13802 .......... .......... .......... .......... .......... .......... 169 GTCATATTGG ATTCTACATC ATTTACATAA TCTCTTTGCA CCAAAAACAT AAACAACACA 13862 .......... .......... .......... .......... .......... .......... 169 AAACAATTCA TCTCTATCAT TTGAATGAGG CTATTGCTGC CTTCAAAACA TCTAGGGTTC 13922 .......... .......... .......... .......... .......... .......... 169 CTTTCCTTCA AAGCTGTCAA CCAAATGCTG GAGTGAGATC TCCCTGATTG TGCCAACATC 13982 .......... .......... .......... .......... .......... .......... 169 TCAGTAAATC AGCTGTAGTT CTTGGCACTG TCCATCCGAG TCCAACTATG CTTGGGAAGA 14042 .......... .......... .......... .......... .......... .......... 169 GTTGCTACAA TTGAGCAGTT ACACCAAGAT GTAAGAACAG GTGTCTGTTA GACTCTTTAA 14102 .......... .......... .......... .......... .......... .......... 169 GTCTGCACAG TATTACTAAC ATCTGGTGCT CAACTGGAAT CCTCTCTTCT TTAAATTTAT 14162 .......... .......... .......... .......... .......... .......... 169 CGAGTTAGAC TGGCCTATAA GAACTTCACT AATGCACCAA AAAGAATCAA GGGATTGAAA 14222 .......... .......... .......... .......... .......... .......... 169 AGCTATTCCT TGAGTCTACA TACTCGTTTT CTATCTCTTA AAAAGTCTTG AACCACTCCT 14282 .......... .......... .......... .......... .......... .......... 169 ATAATGTTGA AGTGATCAAT AATTGAGAAT TTTGTCACAT GTCTTGTTAT GACGCTCATC 14342 .......... .......... .......... .......... .......... .......... 169 ATGTATGCTA GTGGTTTAGA TACTCCTAAG GAAAAGGCAA ACATTTTGAA TCCAAATGTT 14402 .......... .......... .......... .......... .......... .......... 169 AACATGAGGT TTTTTTTTGA GGATAGTAAC ACTAATCTAA ATATTCACAG CTAGACTACA 14462 .......... .......... .......... .......... .......... .......... 169 TCCAAAATAT GTAGTCCCCT TCAAAAGGTA TTGCAGTCTA GTAATATTGC CGTCTATCAC 14522 .......... .......... .......... .......... .......... .......... 169 CTATATTTCT TACAAATAAT CCAAGACATC CCAGATATCT TGCGCTTGGA CTAAAAAGTT 14582 .......... .......... .......... .......... .......... .......... 169 CTCTTTTCAC CAAAAATAAA ATAAAGCTAG AGAATTCATC TTGAAGCTCT GAAGTCCATT 14642 .......... .......... .......... .......... .......... .......... 169 CTGCTTGTCT TCAAAGCACC TCTGGTTAGA GTTTTCTCAA AAGGCCTGCA TATCCACTAT 14702 .......... .......... .......... .......... .......... .......... 169 GGTTCAGGGC CGATCATAAT CCCTATGATG TGATAATTCC GTTGAATAAG GTGGAATGTA 14762 .......... .......... .......... .......... .......... .......... 169 AGTAAAAGAA ATTCATTTAT AAATACCAAT TAAGTGCCAA ACAATGCGTA GATGTCCATG 14822 .......... .......... .......... .......... .......... .......... 169 TTACTTGGAA AAGTTTCCAT ATGATGCTAT ATTTCATGTT CTTTATTCAT TCTTTGTTAT 14882 .......... .......... .......... .......... .......... .......... 169 TTCGTATTAA TTATTTACTT CTTATTACAG CCTTTTATAT AGTTGAGCTT GTGCTAAGTT 14942 .......... .......... .......... .......... .......... .......... 169 GTTTATTCTT TTGCACCATC AGTAATATTC TGAATGTTGC ATGTGATGTA TCAGGTTGAG 15002 .......... .......... .......... .......... .......... .......... 169 GGTAGTTTTG CTATAAAAAT TGATTCCACC TTTATCTTGT CCTAAATTCT AAGTTGGATT 15062 .......... .......... .......... .......... .......... .......... 169 TTCTTTTCTG ATACTCTCAC TACAATCCCT CAGGAAAATC TAGTTATATT GTAAAACTGG 15122 .......... .......... .......... .......... .......... .......... 169 CATATTTGCC TTGAGCTGTC ATATTCTAAC TTGCAGAAAG GAAATAAGAC AAATAGCTCA 15182 .......... .......... .......... .......... .......... .......... 169 TAGTTCCTTA CAAATGCACG CCTTTGGAGC CAGCTGTTAT GTTATTCTCT AAGGCATATC 15242 .......... .......... .......... .......... .......... .......... 169 AGCAGTCAAG ATTGATTCTT AAACTGATTT TTCAGCTCCA TGAATTTGGT CTATTGGTAA 15302 .......... .......... .......... .......... .......... .......... 169 GAGCACATCA TGTAATGTTT GAGTTAGAGG CATGTCACGA GTTTGAACCC AACCACTTCA 15362 .......... .......... .......... .......... .......... .......... 169 AAAAACATAG TTTTTAAGTA TAGAATGTGA GAGGGGCGAC TCCAATATCC ACCGAGTTTT 15422 .......... .......... .......... .......... .......... .......... 169 GGACTGCGTC GGCTAGCCCT CGGGGATTTC TGGGTTATCA ATTTTCTTAA AAAAGCTGCT 15482 .......... .......... .......... .......... .......... .......... 169 ATCTCCATAC TAATTGCTTA TTGAGGGTTA TCAATTTTCT TATAAAAGCT GCTATCTCCA 15542 .......... .......... .......... .......... .......... .......... 169 TACAAATTGC TTATTGAGGA TGAATTTGAG CAGTTACTGA AGCAACTAAT TTTGAATAAT 15602 .......... .......... .......... .......... .......... .......... 169 TTGTCCTTCA AAAATAAAAC CCACATATCA AGAACTAAAT GAAAGGTTAT TGCTTCAGGG 15662 .......... .......... .......... .......... .......... .......... 169 GTGTAGTTGT TCTTTAATAT CTATAAAAGT ATGTGAAAGC AATTTAACCA AGGATTAGTT 15722 .......... .......... .......... .......... .......... .......... 169 TGTTTGACTC GCTGAAGCAA AAGAAGGCTA CTACTTTGGG GCCAGGGTAG CCATAGATAG 15782 .......... .......... .......... .......... .......... .......... 169 TCTGACGTCT TAGAGTATTG CTGTAAACAA TCAACATCTT GCAAAACATG TCATTGTTAA 15842 .......... .......... .......... .......... .......... .......... 169 TGTGTGCCCC CCTGTCTCTC ATACACACAA AAAATTGCCG AAACAGAAAA AAGAATGGTT 15902 .......... .......... .......... .......... .......... .......... 169 GATGAACAGT GCTAGAACCA GCTTTTTTGC AGGGGGAGAC TGATTCACCT TCAAGTGAGC 15962 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .CTTTTTTGC AGGGGGAGAC TGATTCACCT TCAAGTGAGC 208 ATGTGCTTGG CGCAAATGGT GGAAGAAGAT GTTATACATT ACTTCAGGGC CATTCAGGGC 16022 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTGCTTGG CGCAAATGGT GGAAGAAGAT GTTATACATT ACTTCAGGGC CATTCAGGGC 268 CTGTTTATTC TGCATCATTC AGTCCGCACG GGGATTTTCT TCTTTCTTCA TCATCAGATT 16082 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| CTGTTTATTC TGCATCATTC AGTCCTCACG GGGATTTTCT TCTTTCTTCA TCATCAGATT 328 CAACTGGTAT GATATCATAT GACTTTGATT GCTATTAGCT CTACAATAGT TCTTATTCAT 16142 |||||| CAACTG.... .......... .......... .......... .......... .......... 334 ATGGATTATG ATAGTTACTG AAGTTCTATA TGCTTACTCT ATAACCATGT GACCTTTTTG 16202 .......... .......... .......... .......... .......... .......... 334 GCTAATTATT TCTATGCAGT TCGCTTATGG AGCACAAAGT TAAATGCTAA TGTTGTTTGC 16262 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T TCGCTTATGG AGCACAAAGT TAAATGCTAA TGTTGTTTGC 375 TACAAAGGCC ACAATTACCC AGTATGGGAT GTTCAGGTAG GTTGCTAGTT TGTCATTTAT 16322 |||||||||| |||||||||| |||||||||| |||||| TACAAAGGCC ACAATTACCC AGTATGGGAT GTTCAG.... .......... .......... 411 TTTGTTTAAT GATTCCTTTT TGGATGTAAT TGTGGTAATA TATTTGCAAT CTCTTTAAGT 16382 .......... .......... .......... .......... .......... .......... 411 AACTCACCTA ATTGGTTGTT AAACAGAGAA CTGCCCGAGT TAATGCATTT TTTGCTTTCT 16442 .......... .......... .......... .......... .......... .......... 411 CATGGTAACT GTAGATAAAA GTTGTCACAA TGGGCAAATA TATGTTTGGT TTTCACTTCA 16502 .......... .......... .......... .......... .......... .......... 411 CATCTTGAGT GCAGTTTAGC CCAGCTGGAC ATTATTTTGC CAGTTCTTCA CATGACCGCA 16562 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....TTTAGC CCAGCTGGAC ATTATTTTGC CAGTTCTTCA CATGACCGCA 457 CTGCAAGGAT CTGGTCTGTG GACAGGATAC AGCCTCTAAG AATAATGGCC GGACACTTGT 16622 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGCAAGGAT CTGGTCTGTG GACAGGATAC AGCCTCTAAG AATAATGGCC GGACACTTGT 517 CAGATGTAGA TGTGAGTATC TAGTTTGTGC TCTATGCCAT GGGAACTATT TATTTGATCT 16682 |||||||||| | CAGATGTAGA T......... .......... .......... .......... .......... 528 GATCTACGTT GGCATCTTTA TCACTCTGTG GCTGTATAAT CAATCTTAGT TACTTGACCT 16742 .......... .......... .......... .......... .......... .......... 528 ACTTTTCATT TTCGTCCTCT ATAATCACTT GGCCTGTTAT AAAGTGACAT CTTTTCCCCA 16802 .......... .......... .......... .......... .......... .......... 528 TGTAAAAAAA ATGAAATGGC TAAGTTTTCC GAGTGCTCAA ATACCCAGAC ATAGGGGGCC 16862 .......... .......... .......... .......... .......... .......... 528 ATCTTTCCAT TTTGTGAGGC CCCATAATGT TGGCCACCAC TGATACTTTG GCAATTACAA 16922 .......... .......... .......... .......... .......... .......... 528 GAAGTTAAGT TCATTAAGTA TTCTAATTTT TGAACTTCTT GTGATGTTTT CAGGCGTACA 16982 .......... .......... .......... .......... .......... .......... 528 CTTAACTTTT CGACTATCAA TTTAGTTCTT TTCTTAAACT ACTGCTAATA CATTTGATAA 17042 .......... .......... .......... .......... .......... .......... 528 GTTCAAATGA CATCTTAAAG TCCATTCCAG TCAAATATCT CATATAAAAT GGGACAGATG 17102 .......... .......... .......... .......... .......... .......... 528 GAGAGTATAA TGTTTCTTTT AGCTTTGATT TGGTATCTTC TCAACAACAG TCCACACTTC 17162 .......... .......... .......... .......... .......... .......... 528 ATTAACTTTT ACTTTTTAGT GTAACTCTTT CTTTTGCCTT TTTTATCTTT TCACCAACAA 17222 .......... .......... .......... .......... .......... .......... 528 TCCTCCCAAA ATAGTGATGT TTTTTGAAGG ATCCGATTTG ATTGCGGCAA TAATTTTGGA 17282 .......... .......... .......... .......... .......... .......... 528 GAGACTGAGC GCATAGGGAA GAGTAACTTT TTTTATAACT TGGATTATTA ATCTGGGAAG 17342 .......... .......... .......... .......... .......... .......... 528 GCCTTACCTA GTTTGTACCC ATGTCCCATT TGTGACTACT AATTTTGCAA GAAAAGGTTA 17402 .......... .......... .......... .......... .......... .......... 528 AGTTCTATAC ATTATATCAG AGATACACAG ACAATAGACA TTAAATAAAT TACTTAAAAC 17462 .......... .......... .......... .......... .......... .......... 528 TAATTAATGT ATTTAATTAA TTCCAAACAA GTTGTCATGC TTTACTAAAA AGATTGAAGG 17522 .......... .......... .......... .......... .......... .......... 528 TTGAATATTT TATCTGGAGA GATTGGGCAC TAATAAAATG CACTTACCAT AACCATATCA 17582 .......... .......... .......... .......... .......... .......... 528 GGGTAATTTT TGATTTGGCA TGGAAATTTT CAGTTACTTA GATTTCTTGC AATTTCCTTT 17642 .......... .......... .......... .......... .......... .......... 528 CTATTGACAT AAATTTGAGA GGATGTTAAA TTCATCATGT AGTGTGTGCG ATGGCACGCA 17702 |||||||| |||||||||| .......... .......... .......... .......... ..TGTGTGCG ATGGCACGCA 546 AATTGCAATT ACATTGCAAC CGGATCAAGC GACAAAACTG TACGCTTGTG GGACGTCCAG 17762 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATTGCAATT ACATTGCAAC CGGATCAAGC GACAAAACTG TACGCTTGTG GGACGTCCAG 606 AGTGGTGACT GTGTCAGAAT TTTCATTGGT CATAGGAGTA TGATCCTATC ATTGGCAATG 17822 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTGGTGACT GTGTCAGAAT TTTCATTGGT CATAGGAGTA TGATCCTATC ATTGGCAATG 666 TCTCCTGATG GTCGATATAT GGCATCCGGT GATGAAGATG GAACAGTCAT GATGTGGGAT 17882 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCCTGATG GTCGATATAT GGCATCCGGT GATGAAGATG GAACAGTCAT GATGTGGGAT 726 CTCTCAAGTG GACGTTGTAT TACTCCTTTG ACCGGTCATT CCTCTTGTGT GTGGTCACTT 17942 |||||||||| |||||||||| |||||||||| |||||||||| || ||||| |||||||||| CTCTCAAGTG GACGTTGTAT TACTCCTTTG ACCGGTCATT CCCTCTGTGT GTGGTCACTT 786 GC-TTTTAGG TAGGGATAGA AATGAACTTA ACCTTAAGTT TGTGTACGCT CAGAGATGCA 18001 || |||||| GCTTTTTAG. .......... .......... .......... .......... .......... 795 CTTGAGTATG AAGTTCATTG AATATAGATT ATATTTTTGA AGCTTTTTTG TCTTATTTCA 18061 .......... .......... .......... .......... .......... .......... 795 GTGGTGAAGG TTCACTTCTT GCATCGGGCT C 18092 ||||||||| ||||||||| |||||||||| | .TGGTGAAGG -TCACTTCTT GCATCGGGCT C 824 hqPGS_C06HBa0046G10.1-4+_SGN-E262068+ (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17950,18063 18092) ******************************************************************************** EST sequence 10 +strand 572 n (File: SGN-E252040+) 1 TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT GGGTTAATAC ACACAATGGA 61 CTAAACTGTG CATCAATATC ACATGATGGA TCACTAGTGG CTGGTGGATT CTCAGATTCA 121 TCTGTGAAGG TCTGGGACAT GGCAAAGCTT GGGGAACAAA CTGGTCCATC TTTTTTGCAG 181 GGGGAGACTG ATTCACCTTC AAGTGAGCAT GTGCTTGGCG CAAATGGTGG AAGAAGATGT 241 TATACATTAC TTCAGGGCCA TTCAGGGCCT GTTTATTCTG CATCATTCAG TCCTCACGGG 301 GATTTTCTTC TTTCTTCATC ATCAGATTCA ACTGTTCGCT TATGGAGCAC AAAGTTAAAT 361 GCTAATGTTG TTTGCTACAA AGGCCACAAT TACCCAGTAT GGGATGTTCA GTTTAGCCCA 421 GCTGGACATT ATTTTGCCAG TTCTTCACAT GACCGCACTG CAAGGATCTG GTCTGTGGAC 481 AGGATACAGC CTCTAAGAAT AATGGCCGGA CACTTGTCAG ATGTAAATTG TGTGCGATGG 541 CACGCAAATT GCAATTACAT TGCAACCGGA TC Predicted gene structure (within gDNA segment 12183 to 18338): Exon 1 12783 12841 ( 59 n); cDNA 1 59 ( 59 n); score: 0.966 Intron 1 12842 12933 ( 92 n); Pd: 0.980 (s: 0.96), Pa: 0.527 (s: 1.00) Exon 2 12934 13003 ( 70 n); cDNA 60 129 ( 70 n); score: 1.000 Intron 2 13004 13140 ( 137 n); Pd: 0.906 (s: 1.00), Pa: 0.980 (s: 1.00) Exon 3 13141 13180 ( 40 n); cDNA 130 169 ( 40 n); score: 1.000 Intron 3 13181 15923 (2743 n); Pd: 0.995 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 4 15924 16088 ( 165 n); cDNA 170 334 ( 165 n); score: 0.994 Intron 4 16089 16221 ( 133 n); Pd: 0.992 (s: 0.98), Pa: 0.952 (s: 1.00) Exon 5 16222 16298 ( 77 n); cDNA 335 411 ( 77 n); score: 1.000 Intron 5 16299 16516 ( 218 n); Pd: 0.978 (s: 1.00), Pa: 0.764 (s: 1.00) Exon 6 16517 16633 ( 117 n); cDNA 412 528 ( 117 n); score: 0.991 Intron 6 16634 17684 (1051 n); Pd: 0.880 (s: 0.98), Pa: 0.958 (s: 1.00) Exon 7 17685 17728 ( 44 n); cDNA 529 572 ( 44 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E252040+ 0.992 572 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E252040+ (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17728) Alignment (genomic DNA sequence = upper lines): TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT TTGTTAATAC ACACAATGGG 12842 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| ||||||||| TTGAGTAATG CATCATTGCC CTCTGTTAGC TTCTATACAT GGGTTAATAC ACACAATGG. 59 TAAGTTTTCA TGATCTTGAA TCTTTTAAGT GTGGAATTCG TATTCAGGAA CATATCCTAA 12902 .......... .......... .......... .......... .......... .......... 59 TCTCAGTGAG TTAATCTGTA AATGTCTGTA GACTAAACTG TGCATCAATA TCACATGATG 12962 ||||||||| |||||||||| |||||||||| .......... .......... .......... .ACTAAACTG TGCATCAATA TCACATGATG 88 GATCACTAGT GGCTGGTGGA TTCTCAGATT CATCTGTGAA GGTGCTTGTA GCTTCTGAAT 13022 |||||||||| |||||||||| |||||||||| |||||||||| | GATCACTAGT GGCTGGTGGA TTCTCAGATT CATCTGTGAA G......... .......... 129 GACGTTGAAA TTATGTTGTC ACAAATTACA GTATCTGTTG ATCCTTGATC ATCTTCTTAT 13082 .......... .......... .......... .......... .......... .......... 129 CTTTTTTTTG GAACTGGTAA CTTTGATCAT CTTCTAATCT AAGTCCCCCA TTTTACAGGT 13142 || .......... .......... .......... .......... .......... ........GT 131 CTGGGACATG GCAAAGCTTG GGGAACAAAC TGGTCCATGT AAGTCCAATA TGATGGTCCT 13202 |||||||||| |||||||||| |||||||||| |||||||| CTGGGACATG GCAAAGCTTG GGGAACAAAC TGGTCCAT.. .......... .......... 169 CAGCTCCTTT TCAATGTATT GTTATTTCAT GTTTCAAGAT GTTTTAGTGA ATAGAGGTTG 13262 .......... .......... .......... .......... .......... .......... 169 AAAAGGTCCC TTTTAATACT ACTCCTAGTC ACAGTATATC TCGCCAGGAC AGCAAGAAAG 13322 .......... .......... .......... .......... .......... .......... 169 ATTTATTCTT TGCTGCTTTA ATTTATTTGA TAAGTAGATA AATTTTATTT CACTAAGAAA 13382 .......... .......... .......... .......... .......... .......... 169 CTGCACTAGG GTTGTGAAGT TTGTTACATC AGAAATTTGC TTAGCAGAAA CATGATTAGT 13442 .......... .......... .......... .......... .......... .......... 169 TATGAAGGTT TCTCGACTGA ACATATAAAT GCAGCTTTAC ATTTCTGATG GAAGTATTTC 13502 .......... .......... .......... .......... .......... .......... 169 TTGAACTCCT TGCATTTTTC AATTAGGTAA ATAATTTTAA TCGAAAAGTC TCCTGTATAC 13562 .......... .......... .......... .......... .......... .......... 169 AATGCAGTAC ACAAAAAATA ACTATATCAG AATATGATTC TAACAAACAA TATCCTATTG 13622 .......... .......... .......... .......... .......... .......... 169 TTTTTTTTTT GGAGAAAGTA TCAGAATATG ATTCTAACAA ACAATATCCT ACTGTTTTTT 13682 .......... .......... .......... .......... .......... .......... 169 GTTTTTTTTG AGAAAGTAAC ATTTGTATTA AAGAAACTTG GTAAAAAGGT TGTGCTGGTA 13742 .......... .......... .......... .......... .......... .......... 169 ACCATGTGCA GCTTCCAAAA GTATTGGGAA TCCTTTTACT TCTCACTAGG GATCTATCAT 13802 .......... .......... .......... .......... .......... .......... 169 GTCATATTGG ATTCTACATC ATTTACATAA TCTCTTTGCA CCAAAAACAT AAACAACACA 13862 .......... .......... .......... .......... .......... .......... 169 AAACAATTCA TCTCTATCAT TTGAATGAGG CTATTGCTGC CTTCAAAACA TCTAGGGTTC 13922 .......... .......... .......... .......... .......... .......... 169 CTTTCCTTCA AAGCTGTCAA CCAAATGCTG GAGTGAGATC TCCCTGATTG TGCCAACATC 13982 .......... .......... .......... .......... .......... .......... 169 TCAGTAAATC AGCTGTAGTT CTTGGCACTG TCCATCCGAG TCCAACTATG CTTGGGAAGA 14042 .......... .......... .......... .......... .......... .......... 169 GTTGCTACAA TTGAGCAGTT ACACCAAGAT GTAAGAACAG GTGTCTGTTA GACTCTTTAA 14102 .......... .......... .......... .......... .......... .......... 169 GTCTGCACAG TATTACTAAC ATCTGGTGCT CAACTGGAAT CCTCTCTTCT TTAAATTTAT 14162 .......... .......... .......... .......... .......... .......... 169 CGAGTTAGAC TGGCCTATAA GAACTTCACT AATGCACCAA AAAGAATCAA GGGATTGAAA 14222 .......... .......... .......... .......... .......... .......... 169 AGCTATTCCT TGAGTCTACA TACTCGTTTT CTATCTCTTA AAAAGTCTTG AACCACTCCT 14282 .......... .......... .......... .......... .......... .......... 169 ATAATGTTGA AGTGATCAAT AATTGAGAAT TTTGTCACAT GTCTTGTTAT GACGCTCATC 14342 .......... .......... .......... .......... .......... .......... 169 ATGTATGCTA GTGGTTTAGA TACTCCTAAG GAAAAGGCAA ACATTTTGAA TCCAAATGTT 14402 .......... .......... .......... .......... .......... .......... 169 AACATGAGGT TTTTTTTTGA GGATAGTAAC ACTAATCTAA ATATTCACAG CTAGACTACA 14462 .......... .......... .......... .......... .......... .......... 169 TCCAAAATAT GTAGTCCCCT TCAAAAGGTA TTGCAGTCTA GTAATATTGC CGTCTATCAC 14522 .......... .......... .......... .......... .......... .......... 169 CTATATTTCT TACAAATAAT CCAAGACATC CCAGATATCT TGCGCTTGGA CTAAAAAGTT 14582 .......... .......... .......... .......... .......... .......... 169 CTCTTTTCAC CAAAAATAAA ATAAAGCTAG AGAATTCATC TTGAAGCTCT GAAGTCCATT 14642 .......... .......... .......... .......... .......... .......... 169 CTGCTTGTCT TCAAAGCACC TCTGGTTAGA GTTTTCTCAA AAGGCCTGCA TATCCACTAT 14702 .......... .......... .......... .......... .......... .......... 169 GGTTCAGGGC CGATCATAAT CCCTATGATG TGATAATTCC GTTGAATAAG GTGGAATGTA 14762 .......... .......... .......... .......... .......... .......... 169 AGTAAAAGAA ATTCATTTAT AAATACCAAT TAAGTGCCAA ACAATGCGTA GATGTCCATG 14822 .......... .......... .......... .......... .......... .......... 169 TTACTTGGAA AAGTTTCCAT ATGATGCTAT ATTTCATGTT CTTTATTCAT TCTTTGTTAT 14882 .......... .......... .......... .......... .......... .......... 169 TTCGTATTAA TTATTTACTT CTTATTACAG CCTTTTATAT AGTTGAGCTT GTGCTAAGTT 14942 .......... .......... .......... .......... .......... .......... 169 GTTTATTCTT TTGCACCATC AGTAATATTC TGAATGTTGC ATGTGATGTA TCAGGTTGAG 15002 .......... .......... .......... .......... .......... .......... 169 GGTAGTTTTG CTATAAAAAT TGATTCCACC TTTATCTTGT CCTAAATTCT AAGTTGGATT 15062 .......... .......... .......... .......... .......... .......... 169 TTCTTTTCTG ATACTCTCAC TACAATCCCT CAGGAAAATC TAGTTATATT GTAAAACTGG 15122 .......... .......... .......... .......... .......... .......... 169 CATATTTGCC TTGAGCTGTC ATATTCTAAC TTGCAGAAAG GAAATAAGAC AAATAGCTCA 15182 .......... .......... .......... .......... .......... .......... 169 TAGTTCCTTA CAAATGCACG CCTTTGGAGC CAGCTGTTAT GTTATTCTCT AAGGCATATC 15242 .......... .......... .......... .......... .......... .......... 169 AGCAGTCAAG ATTGATTCTT AAACTGATTT TTCAGCTCCA TGAATTTGGT CTATTGGTAA 15302 .......... .......... .......... .......... .......... .......... 169 GAGCACATCA TGTAATGTTT GAGTTAGAGG CATGTCACGA GTTTGAACCC AACCACTTCA 15362 .......... .......... .......... .......... .......... .......... 169 AAAAACATAG TTTTTAAGTA TAGAATGTGA GAGGGGCGAC TCCAATATCC ACCGAGTTTT 15422 .......... .......... .......... .......... .......... .......... 169 GGACTGCGTC GGCTAGCCCT CGGGGATTTC TGGGTTATCA ATTTTCTTAA AAAAGCTGCT 15482 .......... .......... .......... .......... .......... .......... 169 ATCTCCATAC TAATTGCTTA TTGAGGGTTA TCAATTTTCT TATAAAAGCT GCTATCTCCA 15542 .......... .......... .......... .......... .......... .......... 169 TACAAATTGC TTATTGAGGA TGAATTTGAG CAGTTACTGA AGCAACTAAT TTTGAATAAT 15602 .......... .......... .......... .......... .......... .......... 169 TTGTCCTTCA AAAATAAAAC CCACATATCA AGAACTAAAT GAAAGGTTAT TGCTTCAGGG 15662 .......... .......... .......... .......... .......... .......... 169 GTGTAGTTGT TCTTTAATAT CTATAAAAGT ATGTGAAAGC AATTTAACCA AGGATTAGTT 15722 .......... .......... .......... .......... .......... .......... 169 TGTTTGACTC GCTGAAGCAA AAGAAGGCTA CTACTTTGGG GCCAGGGTAG CCATAGATAG 15782 .......... .......... .......... .......... .......... .......... 169 TCTGACGTCT TAGAGTATTG CTGTAAACAA TCAACATCTT GCAAAACATG TCATTGTTAA 15842 .......... .......... .......... .......... .......... .......... 169 TGTGTGCCCC CCTGTCTCTC ATACACACAA AAAATTGCCG AAACAGAAAA AAGAATGGTT 15902 .......... .......... .......... .......... .......... .......... 169 GATGAACAGT GCTAGAACCA GCTTTTTTGC AGGGGGAGAC TGATTCACCT TCAAGTGAGC 15962 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .CTTTTTTGC AGGGGGAGAC TGATTCACCT TCAAGTGAGC 208 ATGTGCTTGG CGCAAATGGT GGAAGAAGAT GTTATACATT ACTTCAGGGC CATTCAGGGC 16022 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTGCTTGG CGCAAATGGT GGAAGAAGAT GTTATACATT ACTTCAGGGC CATTCAGGGC 268 CTGTTTATTC TGCATCATTC AGTCCGCACG GGGATTTTCT TCTTTCTTCA TCATCAGATT 16082 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| CTGTTTATTC TGCATCATTC AGTCCTCACG GGGATTTTCT TCTTTCTTCA TCATCAGATT 328 CAACTGGTAT GATATCATAT GACTTTGATT GCTATTAGCT CTACAATAGT TCTTATTCAT 16142 |||||| CAACTG.... .......... .......... .......... .......... .......... 334 ATGGATTATG ATAGTTACTG AAGTTCTATA TGCTTACTCT ATAACCATGT GACCTTTTTG 16202 .......... .......... .......... .......... .......... .......... 334 GCTAATTATT TCTATGCAGT TCGCTTATGG AGCACAAAGT TAAATGCTAA TGTTGTTTGC 16262 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T TCGCTTATGG AGCACAAAGT TAAATGCTAA TGTTGTTTGC 375 TACAAAGGCC ACAATTACCC AGTATGGGAT GTTCAGGTAG GTTGCTAGTT TGTCATTTAT 16322 |||||||||| |||||||||| |||||||||| |||||| TACAAAGGCC ACAATTACCC AGTATGGGAT GTTCAG.... .......... .......... 411 TTTGTTTAAT GATTCCTTTT TGGATGTAAT TGTGGTAATA TATTTGCAAT CTCTTTAAGT 16382 .......... .......... .......... .......... .......... .......... 411 AACTCACCTA ATTGGTTGTT AAACAGAGAA CTGCCCGAGT TAATGCATTT TTTGCTTTCT 16442 .......... .......... .......... .......... .......... .......... 411 CATGGTAACT GTAGATAAAA GTTGTCACAA TGGGCAAATA TATGTTTGGT TTTCACTTCA 16502 .......... .......... .......... .......... .......... .......... 411 CATCTTGAGT GCAGTTTAGC CCAGCTGGAC ATTATTTTGC CAGTTCTTCA CATGACCGCA 16562 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....TTTAGC CCAGCTGGAC ATTATTTTGC CAGTTCTTCA CATGACCGCA 457 CTGCAAGGAT CTGGTCTGTG GACAGGATAC AGCCTCTAAG AATAATGGCC GGACACTTGT 16622 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGCAAGGAT CTGGTCTGTG GACAGGATAC AGCCTCTAAG AATAATGGCC GGACACTTGT 517 CAGATGTAGA TGTGAGTATC TAGTTTGTGC TCTATGCCAT GGGAACTATT TATTTGATCT 16682 |||||||| | | CAGATGTAAA T......... .......... .......... .......... .......... 528 GATCTACGTT GGCATCTTTA TCACTCTGTG GCTGTATAAT CAATCTTAGT TACTTGACCT 16742 .......... .......... .......... .......... .......... .......... 528 ACTTTTCATT TTCGTCCTCT ATAATCACTT GGCCTGTTAT AAAGTGACAT CTTTTCCCCA 16802 .......... .......... .......... .......... .......... .......... 528 TGTAAAAAAA ATGAAATGGC TAAGTTTTCC GAGTGCTCAA ATACCCAGAC ATAGGGGGCC 16862 .......... .......... .......... .......... .......... .......... 528 ATCTTTCCAT TTTGTGAGGC CCCATAATGT TGGCCACCAC TGATACTTTG GCAATTACAA 16922 .......... .......... .......... .......... .......... .......... 528 GAAGTTAAGT TCATTAAGTA TTCTAATTTT TGAACTTCTT GTGATGTTTT CAGGCGTACA 16982 .......... .......... .......... .......... .......... .......... 528 CTTAACTTTT CGACTATCAA TTTAGTTCTT TTCTTAAACT ACTGCTAATA CATTTGATAA 17042 .......... .......... .......... .......... .......... .......... 528 GTTCAAATGA CATCTTAAAG TCCATTCCAG TCAAATATCT CATATAAAAT GGGACAGATG 17102 .......... .......... .......... .......... .......... .......... 528 GAGAGTATAA TGTTTCTTTT AGCTTTGATT TGGTATCTTC TCAACAACAG TCCACACTTC 17162 .......... .......... .......... .......... .......... .......... 528 ATTAACTTTT ACTTTTTAGT GTAACTCTTT CTTTTGCCTT TTTTATCTTT TCACCAACAA 17222 .......... .......... .......... .......... .......... .......... 528 TCCTCCCAAA ATAGTGATGT TTTTTGAAGG ATCCGATTTG ATTGCGGCAA TAATTTTGGA 17282 .......... .......... .......... .......... .......... .......... 528 GAGACTGAGC GCATAGGGAA GAGTAACTTT TTTTATAACT TGGATTATTA ATCTGGGAAG 17342 .......... .......... .......... .......... .......... .......... 528 GCCTTACCTA GTTTGTACCC ATGTCCCATT TGTGACTACT AATTTTGCAA GAAAAGGTTA 17402 .......... .......... .......... .......... .......... .......... 528 AGTTCTATAC ATTATATCAG AGATACACAG ACAATAGACA TTAAATAAAT TACTTAAAAC 17462 .......... .......... .......... .......... .......... .......... 528 TAATTAATGT ATTTAATTAA TTCCAAACAA GTTGTCATGC TTTACTAAAA AGATTGAAGG 17522 .......... .......... .......... .......... .......... .......... 528 TTGAATATTT TATCTGGAGA GATTGGGCAC TAATAAAATG CACTTACCAT AACCATATCA 17582 .......... .......... .......... .......... .......... .......... 528 GGGTAATTTT TGATTTGGCA TGGAAATTTT CAGTTACTTA GATTTCTTGC AATTTCCTTT 17642 .......... .......... .......... .......... .......... .......... 528 CTATTGACAT AAATTTGAGA GGATGTTAAA TTCATCATGT AGTGTGTGCG ATGGCACGCA 17702 |||||||| |||||||||| .......... .......... .......... .......... ..TGTGTGCG ATGGCACGCA 546 AATTGCAATT ACATTGCAAC CGGATC 17728 |||||||||| |||||||||| |||||| AATTGCAATT ACATTGCAAC CGGATC 572 hqPGS_C06HBa0046G10.1-4+_SGN-E252040+ (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17728) ******************************************************************************** EST sequence 4 +strand 496 n (File: SGN-E251386+) 1 GTGGATTCTC AGATTCATCT GTGAAGGTCT GGGACATGGC AAAGCTTGGG GAACAAACTG 61 GTCCATCTTT TTTGCAGGGG GAGACTGATT CACCTTCAAG TGAGCATGTG CTTGGCGCAA 121 ATGGTGGAAG AAGATGTTAT ACATTACTTC AGGGCCATTC AGGGCCTGTT TATTCTGCAT 181 CATTCAGTCC GCACGGGGAT TTTCTTCTTT CTTCATCATC AGATTCAACT GTTCGCTTAT 241 GGAGCACAAA GTTAAATGCT AATGTTGTTT GCTACAAAGG CCACAATTAC CCAGTATGGG 301 ATGTTCAGTT TAGCCCAGCT GGACATTATT TTGCCAGTTC TTCACATGAC CGCACTGCAA 361 GGATCTGGTC TGTGGACAGG ATACAGCCTC TAAGAATAAT GGCCGGACAC TTGTCAGATG 421 TAGATTGTGT GCGATGGCAC GCAAATTGCA ATTACATTGC AACCGGATCA AGCGACAAAA 481 CTGTACGCTT GTGGGA Predicted gene structure (within gDNA segment 12299 to 18365): Exon 1 12978 13003 ( 26 n); cDNA 1 26 ( 26 n); score: 1.000 Intron 1 13004 13140 ( 137 n); Pd: 0.906 (s: 0), Pa: 0.980 (s: 1.00) Exon 2 13141 13180 ( 40 n); cDNA 27 66 ( 40 n); score: 1.000 Intron 2 13181 15923 (2743 n); Pd: 0.995 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 15924 16088 ( 165 n); cDNA 67 231 ( 165 n); score: 1.000 Intron 3 16089 16221 ( 133 n); Pd: 0.992 (s: 1.00), Pa: 0.952 (s: 1.00) Exon 4 16222 16298 ( 77 n); cDNA 232 308 ( 77 n); score: 1.000 Intron 4 16299 16516 ( 218 n); Pd: 0.978 (s: 1.00), Pa: 0.764 (s: 1.00) Exon 5 16517 16633 ( 117 n); cDNA 309 425 ( 117 n); score: 1.000 Intron 5 16634 17684 (1051 n); Pd: 0.880 (s: 1.00), Pa: 0.958 (s: 1.00) Exon 6 17685 17755 ( 71 n); cDNA 426 496 ( 71 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E251386+ 1.000 496 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E251386+ (12978 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17755) Alignment (genomic DNA sequence = upper lines): GTGGATTCTC AGATTCATCT GTGAAGGTGC TTGTAGCTTC TGAATGACGT TGAAATTATG 13037 |||||||||| |||||||||| |||||| GTGGATTCTC AGATTCATCT GTGAAG.... .......... .......... .......... 26 TTGTCACAAA TTACAGTATC TGTTGATCCT TGATCATCTT CTTATCTTTT TTTTGGAACT 13097 .......... .......... .......... .......... .......... .......... 26 GGTAACTTTG ATCATCTTCT AATCTAAGTC CCCCATTTTA CAGGTCTGGG ACATGGCAAA 13157 ||||||| |||||||||| .......... .......... .......... .......... ...GTCTGGG ACATGGCAAA 43 GCTTGGGGAA CAAACTGGTC CATGTAAGTC CAATATGATG GTCCTCAGCT CCTTTTCAAT 13217 |||||||||| |||||||||| ||| GCTTGGGGAA CAAACTGGTC CAT....... .......... .......... .......... 66 GTATTGTTAT TTCATGTTTC AAGATGTTTT AGTGAATAGA GGTTGAAAAG GTCCCTTTTA 13277 .......... .......... .......... .......... .......... .......... 66 ATACTACTCC TAGTCACAGT ATATCTCGCC AGGACAGCAA GAAAGATTTA TTCTTTGCTG 13337 .......... .......... .......... .......... .......... .......... 66 CTTTAATTTA TTTGATAAGT AGATAAATTT TATTTCACTA AGAAACTGCA CTAGGGTTGT 13397 .......... .......... .......... .......... .......... .......... 66 GAAGTTTGTT ACATCAGAAA TTTGCTTAGC AGAAACATGA TTAGTTATGA AGGTTTCTCG 13457 .......... .......... .......... .......... .......... .......... 66 ACTGAACATA TAAATGCAGC TTTACATTTC TGATGGAAGT ATTTCTTGAA CTCCTTGCAT 13517 .......... .......... .......... .......... .......... .......... 66 TTTTCAATTA GGTAAATAAT TTTAATCGAA AAGTCTCCTG TATACAATGC AGTACACAAA 13577 .......... .......... .......... .......... .......... .......... 66 AAATAACTAT ATCAGAATAT GATTCTAACA AACAATATCC TATTGTTTTT TTTTTGGAGA 13637 .......... .......... .......... .......... .......... .......... 66 AAGTATCAGA ATATGATTCT AACAAACAAT ATCCTACTGT TTTTTGTTTT TTTTGAGAAA 13697 .......... .......... .......... .......... .......... .......... 66 GTAACATTTG TATTAAAGAA ACTTGGTAAA AAGGTTGTGC TGGTAACCAT GTGCAGCTTC 13757 .......... .......... .......... .......... .......... .......... 66 CAAAAGTATT GGGAATCCTT TTACTTCTCA CTAGGGATCT ATCATGTCAT ATTGGATTCT 13817 .......... .......... .......... .......... .......... .......... 66 ACATCATTTA CATAATCTCT TTGCACCAAA AACATAAACA ACACAAAACA ATTCATCTCT 13877 .......... .......... .......... .......... .......... .......... 66 ATCATTTGAA TGAGGCTATT GCTGCCTTCA AAACATCTAG GGTTCCTTTC CTTCAAAGCT 13937 .......... .......... .......... .......... .......... .......... 66 GTCAACCAAA TGCTGGAGTG AGATCTCCCT GATTGTGCCA ACATCTCAGT AAATCAGCTG 13997 .......... .......... .......... .......... .......... .......... 66 TAGTTCTTGG CACTGTCCAT CCGAGTCCAA CTATGCTTGG GAAGAGTTGC TACAATTGAG 14057 .......... .......... .......... .......... .......... .......... 66 CAGTTACACC AAGATGTAAG AACAGGTGTC TGTTAGACTC TTTAAGTCTG CACAGTATTA 14117 .......... .......... .......... .......... .......... .......... 66 CTAACATCTG GTGCTCAACT GGAATCCTCT CTTCTTTAAA TTTATCGAGT TAGACTGGCC 14177 .......... .......... .......... .......... .......... .......... 66 TATAAGAACT TCACTAATGC ACCAAAAAGA ATCAAGGGAT TGAAAAGCTA TTCCTTGAGT 14237 .......... .......... .......... .......... .......... .......... 66 CTACATACTC GTTTTCTATC TCTTAAAAAG TCTTGAACCA CTCCTATAAT GTTGAAGTGA 14297 .......... .......... .......... .......... .......... .......... 66 TCAATAATTG AGAATTTTGT CACATGTCTT GTTATGACGC TCATCATGTA TGCTAGTGGT 14357 .......... .......... .......... .......... .......... .......... 66 TTAGATACTC CTAAGGAAAA GGCAAACATT TTGAATCCAA ATGTTAACAT GAGGTTTTTT 14417 .......... .......... .......... .......... .......... .......... 66 TTTGAGGATA GTAACACTAA TCTAAATATT CACAGCTAGA CTACATCCAA AATATGTAGT 14477 .......... .......... .......... .......... .......... .......... 66 CCCCTTCAAA AGGTATTGCA GTCTAGTAAT ATTGCCGTCT ATCACCTATA TTTCTTACAA 14537 .......... .......... .......... .......... .......... .......... 66 ATAATCCAAG ACATCCCAGA TATCTTGCGC TTGGACTAAA AAGTTCTCTT TTCACCAAAA 14597 .......... .......... .......... .......... .......... .......... 66 ATAAAATAAA GCTAGAGAAT TCATCTTGAA GCTCTGAAGT CCATTCTGCT TGTCTTCAAA 14657 .......... .......... .......... .......... .......... .......... 66 GCACCTCTGG TTAGAGTTTT CTCAAAAGGC CTGCATATCC ACTATGGTTC AGGGCCGATC 14717 .......... .......... .......... .......... .......... .......... 66 ATAATCCCTA TGATGTGATA ATTCCGTTGA ATAAGGTGGA ATGTAAGTAA AAGAAATTCA 14777 .......... .......... .......... .......... .......... .......... 66 TTTATAAATA CCAATTAAGT GCCAAACAAT GCGTAGATGT CCATGTTACT TGGAAAAGTT 14837 .......... .......... .......... .......... .......... .......... 66 TCCATATGAT GCTATATTTC ATGTTCTTTA TTCATTCTTT GTTATTTCGT ATTAATTATT 14897 .......... .......... .......... .......... .......... .......... 66 TACTTCTTAT TACAGCCTTT TATATAGTTG AGCTTGTGCT AAGTTGTTTA TTCTTTTGCA 14957 .......... .......... .......... .......... .......... .......... 66 CCATCAGTAA TATTCTGAAT GTTGCATGTG ATGTATCAGG TTGAGGGTAG TTTTGCTATA 15017 .......... .......... .......... .......... .......... .......... 66 AAAATTGATT CCACCTTTAT CTTGTCCTAA ATTCTAAGTT GGATTTTCTT TTCTGATACT 15077 .......... .......... .......... .......... .......... .......... 66 CTCACTACAA TCCCTCAGGA AAATCTAGTT ATATTGTAAA ACTGGCATAT TTGCCTTGAG 15137 .......... .......... .......... .......... .......... .......... 66 CTGTCATATT CTAACTTGCA GAAAGGAAAT AAGACAAATA GCTCATAGTT CCTTACAAAT 15197 .......... .......... .......... .......... .......... .......... 66 GCACGCCTTT GGAGCCAGCT GTTATGTTAT TCTCTAAGGC ATATCAGCAG TCAAGATTGA 15257 .......... .......... .......... .......... .......... .......... 66 TTCTTAAACT GATTTTTCAG CTCCATGAAT TTGGTCTATT GGTAAGAGCA CATCATGTAA 15317 .......... .......... .......... .......... .......... .......... 66 TGTTTGAGTT AGAGGCATGT CACGAGTTTG AACCCAACCA CTTCAAAAAA CATAGTTTTT 15377 .......... .......... .......... .......... .......... .......... 66 AAGTATAGAA TGTGAGAGGG GCGACTCCAA TATCCACCGA GTTTTGGACT GCGTCGGCTA 15437 .......... .......... .......... .......... .......... .......... 66 GCCCTCGGGG ATTTCTGGGT TATCAATTTT CTTAAAAAAG CTGCTATCTC CATACTAATT 15497 .......... .......... .......... .......... .......... .......... 66 GCTTATTGAG GGTTATCAAT TTTCTTATAA AAGCTGCTAT CTCCATACAA ATTGCTTATT 15557 .......... .......... .......... .......... .......... .......... 66 GAGGATGAAT TTGAGCAGTT ACTGAAGCAA CTAATTTTGA ATAATTTGTC CTTCAAAAAT 15617 .......... .......... .......... .......... .......... .......... 66 AAAACCCACA TATCAAGAAC TAAATGAAAG GTTATTGCTT CAGGGGTGTA GTTGTTCTTT 15677 .......... .......... .......... .......... .......... .......... 66 AATATCTATA AAAGTATGTG AAAGCAATTT AACCAAGGAT TAGTTTGTTT GACTCGCTGA 15737 .......... .......... .......... .......... .......... .......... 66 AGCAAAAGAA GGCTACTACT TTGGGGCCAG GGTAGCCATA GATAGTCTGA CGTCTTAGAG 15797 .......... .......... .......... .......... .......... .......... 66 TATTGCTGTA AACAATCAAC ATCTTGCAAA ACATGTCATT GTTAATGTGT GCCCCCCTGT 15857 .......... .......... .......... .......... .......... .......... 66 CTCTCATACA CACAAAAAAT TGCCGAAACA GAAAAAAGAA TGGTTGATGA ACAGTGCTAG 15917 .......... .......... .......... .......... .......... .......... 66 AACCAGCTTT TTTGCAGGGG GAGACTGATT CACCTTCAAG TGAGCATGTG CTTGGCGCAA 15977 |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ......CTTT TTTGCAGGGG GAGACTGATT CACCTTCAAG TGAGCATGTG CTTGGCGCAA 120 ATGGTGGAAG AAGATGTTAT ACATTACTTC AGGGCCATTC AGGGCCTGTT TATTCTGCAT 16037 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGGTGGAAG AAGATGTTAT ACATTACTTC AGGGCCATTC AGGGCCTGTT TATTCTGCAT 180 CATTCAGTCC GCACGGGGAT TTTCTTCTTT CTTCATCATC AGATTCAACT GGTATGATAT 16097 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CATTCAGTCC GCACGGGGAT TTTCTTCTTT CTTCATCATC AGATTCAACT G......... 231 CATATGACTT TGATTGCTAT TAGCTCTACA ATAGTTCTTA TTCATATGGA TTATGATAGT 16157 .......... .......... .......... .......... .......... .......... 231 TACTGAAGTT CTATATGCTT ACTCTATAAC CATGTGACCT TTTTGGCTAA TTATTTCTAT 16217 .......... .......... .......... .......... .......... .......... 231 GCAGTTCGCT TATGGAGCAC AAAGTTAAAT GCTAATGTTG TTTGCTACAA AGGCCACAAT 16277 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....TTCGCT TATGGAGCAC AAAGTTAAAT GCTAATGTTG TTTGCTACAA AGGCCACAAT 287 TACCCAGTAT GGGATGTTCA GGTAGGTTGC TAGTTTGTCA TTTATTTTGT TTAATGATTC 16337 |||||||||| |||||||||| | TACCCAGTAT GGGATGTTCA G......... .......... .......... .......... 308 CTTTTTGGAT GTAATTGTGG TAATATATTT GCAATCTCTT TAAGTAACTC ACCTAATTGG 16397 .......... .......... .......... .......... .......... .......... 308 TTGTTAAACA GAGAACTGCC CGAGTTAATG CATTTTTTGC TTTCTCATGG TAACTGTAGA 16457 .......... .......... .......... .......... .......... .......... 308 TAAAAGTTGT CACAATGGGC AAATATATGT TTGGTTTTCA CTTCACATCT TGAGTGCAGT 16517 | .......... .......... .......... .......... .......... .........T 309 TTAGCCCAGC TGGACATTAT TTTGCCAGTT CTTCACATGA CCGCACTGCA AGGATCTGGT 16577 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAGCCCAGC TGGACATTAT TTTGCCAGTT CTTCACATGA CCGCACTGCA AGGATCTGGT 369 CTGTGGACAG GATACAGCCT CTAAGAATAA TGGCCGGACA CTTGTCAGAT GTAGATGTGA 16637 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| CTGTGGACAG GATACAGCCT CTAAGAATAA TGGCCGGACA CTTGTCAGAT GTAGAT.... 425 GTATCTAGTT TGTGCTCTAT GCCATGGGAA CTATTTATTT GATCTGATCT ACGTTGGCAT 16697 .......... .......... .......... .......... .......... .......... 425 CTTTATCACT CTGTGGCTGT ATAATCAATC TTAGTTACTT GACCTACTTT TCATTTTCGT 16757 .......... .......... .......... .......... .......... .......... 425 CCTCTATAAT CACTTGGCCT GTTATAAAGT GACATCTTTT CCCCATGTAA AAAAAATGAA 16817 .......... .......... .......... .......... .......... .......... 425 ATGGCTAAGT TTTCCGAGTG CTCAAATACC CAGACATAGG GGGCCATCTT TCCATTTTGT 16877 .......... .......... .......... .......... .......... .......... 425 GAGGCCCCAT AATGTTGGCC ACCACTGATA CTTTGGCAAT TACAAGAAGT TAAGTTCATT 16937 .......... .......... .......... .......... .......... .......... 425 AAGTATTCTA ATTTTTGAAC TTCTTGTGAT GTTTTCAGGC GTACACTTAA CTTTTCGACT 16997 .......... .......... .......... .......... .......... .......... 425 ATCAATTTAG TTCTTTTCTT AAACTACTGC TAATACATTT GATAAGTTCA AATGACATCT 17057 .......... .......... .......... .......... .......... .......... 425 TAAAGTCCAT TCCAGTCAAA TATCTCATAT AAAATGGGAC AGATGGAGAG TATAATGTTT 17117 .......... .......... .......... .......... .......... .......... 425 CTTTTAGCTT TGATTTGGTA TCTTCTCAAC AACAGTCCAC ACTTCATTAA CTTTTACTTT 17177 .......... .......... .......... .......... .......... .......... 425 TTAGTGTAAC TCTTTCTTTT GCCTTTTTTA TCTTTTCACC AACAATCCTC CCAAAATAGT 17237 .......... .......... .......... .......... .......... .......... 425 GATGTTTTTT GAAGGATCCG ATTTGATTGC GGCAATAATT TTGGAGAGAC TGAGCGCATA 17297 .......... .......... .......... .......... .......... .......... 425 GGGAAGAGTA ACTTTTTTTA TAACTTGGAT TATTAATCTG GGAAGGCCTT ACCTAGTTTG 17357 .......... .......... .......... .......... .......... .......... 425 TACCCATGTC CCATTTGTGA CTACTAATTT TGCAAGAAAA GGTTAAGTTC TATACATTAT 17417 .......... .......... .......... .......... .......... .......... 425 ATCAGAGATA CACAGACAAT AGACATTAAA TAAATTACTT AAAACTAATT AATGTATTTA 17477 .......... .......... .......... .......... .......... .......... 425 ATTAATTCCA AACAAGTTGT CATGCTTTAC TAAAAAGATT GAAGGTTGAA TATTTTATCT 17537 .......... .......... .......... .......... .......... .......... 425 GGAGAGATTG GGCACTAATA AAATGCACTT ACCATAACCA TATCAGGGTA ATTTTTGATT 17597 .......... .......... .......... .......... .......... .......... 425 TGGCATGGAA ATTTTCAGTT ACTTAGATTT CTTGCAATTT CCTTTCTATT GACATAAATT 17657 .......... .......... .......... .......... .......... .......... 425 TGAGAGGATG TTAAATTCAT CATGTAGTGT GTGCGATGGC ACGCAAATTG CAATTACATT 17717 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......TGT GTGCGATGGC ACGCAAATTG CAATTACATT 458 GCAACCGGAT CAAGCGACAA AACTGTACGC TTGTGGGA 17755 |||||||||| |||||||||| |||||||||| |||||||| GCAACCGGAT CAAGCGACAA AACTGTACGC TTGTGGGA 496 hqPGS_C06HBa0046G10.1-4+_SGN-E251386+ (12978 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17755) ******************************************************************************** EST sequence 5 +strand 503 n (File: SGN-E203439+) 1 TCTGGTCTGT GGACAGGATA CAGCCTCTAA GAATAATGGC CGGACACTTG TCAGATGTAG 61 ATTGTGTGCG ATGGCACGCA AATTGCAATT ACATTGCAAC CGGATCAAGC GACAAAACTG 121 TACGCTTGTG GGACGTCCAG AGTGGTGACT GTGTCAGAAT TTTCATTGGT CATAGGAGTA 181 TGATCCTATC ATTGGCAATG TCTCCTGATG GTCGATATAT GGCATCCGGT GATGAAGATG 241 GAACAGTCAT GATGTGGGAT CTCTCAAGTG GACGTTGTAT TACTCCTTTG ACCGGTCATT 301 CCTCTTGTGT GTGGTCACTT GCTTTTAGTG GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG 361 CGGATTGCAC TGTAAAATTA TGGGATGTAA CCACAAGCAC CAGAGCGCCA CTAAAAGAAG 421 AGAACAAATC TGGAGGTAAC AATAGGCTTC GGTTATTGAA GACCCTACCT ACCAAAGCTA 481 CACCAATCTA TGCTTTGCAC TTT Predicted gene structure (within gDNA segment 15972 to 18967): Exon 1 16572 16633 ( 62 n); cDNA 1 62 ( 62 n); score: 1.000 Intron 1 16634 17684 (1051 n); Pd: 0.880 (s: 1.00), Pa: 0.958 (s: 1.00) Exon 2 17685 17950 ( 266 n); cDNA 63 328 ( 266 n); score: 1.000 Intron 2 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 3 18063 18158 ( 96 n); cDNA 329 424 ( 96 n); score: 1.000 Intron 3 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 4 18252 18327 ( 76 n); cDNA 425 500 ( 76 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E203439+ 1.000 500 0.994 C PGS_C06HBa0046G10.1-4+_SGN-E203439+ (16572 16633,17685 17950,18063 18158,18252 18327) Alignment (genomic DNA sequence = upper lines): TCTGGTCTGT GGACAGGATA CAGCCTCTAA GAATAATGGC CGGACACTTG TCAGATGTAG 16631 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGGTCTGT GGACAGGATA CAGCCTCTAA GAATAATGGC CGGACACTTG TCAGATGTAG 60 ATGTGAGTAT CTAGTTTGTG CTCTATGCCA TGGGAACTAT TTATTTGATC TGATCTACGT 16691 || AT........ .......... .......... .......... .......... .......... 62 TGGCATCTTT ATCACTCTGT GGCTGTATAA TCAATCTTAG TTACTTGACC TACTTTTCAT 16751 .......... .......... .......... .......... .......... .......... 62 TTTCGTCCTC TATAATCACT TGGCCTGTTA TAAAGTGACA TCTTTTCCCC ATGTAAAAAA 16811 .......... .......... .......... .......... .......... .......... 62 AATGAAATGG CTAAGTTTTC CGAGTGCTCA AATACCCAGA CATAGGGGGC CATCTTTCCA 16871 .......... .......... .......... .......... .......... .......... 62 TTTTGTGAGG CCCCATAATG TTGGCCACCA CTGATACTTT GGCAATTACA AGAAGTTAAG 16931 .......... .......... .......... .......... .......... .......... 62 TTCATTAAGT ATTCTAATTT TTGAACTTCT TGTGATGTTT TCAGGCGTAC ACTTAACTTT 16991 .......... .......... .......... .......... .......... .......... 62 TCGACTATCA ATTTAGTTCT TTTCTTAAAC TACTGCTAAT ACATTTGATA AGTTCAAATG 17051 .......... .......... .......... .......... .......... .......... 62 ACATCTTAAA GTCCATTCCA GTCAAATATC TCATATAAAA TGGGACAGAT GGAGAGTATA 17111 .......... .......... .......... .......... .......... .......... 62 ATGTTTCTTT TAGCTTTGAT TTGGTATCTT CTCAACAACA GTCCACACTT CATTAACTTT 17171 .......... .......... .......... .......... .......... .......... 62 TACTTTTTAG TGTAACTCTT TCTTTTGCCT TTTTTATCTT TTCACCAACA ATCCTCCCAA 17231 .......... .......... .......... .......... .......... .......... 62 AATAGTGATG TTTTTTGAAG GATCCGATTT GATTGCGGCA ATAATTTTGG AGAGACTGAG 17291 .......... .......... .......... .......... .......... .......... 62 CGCATAGGGA AGAGTAACTT TTTTTATAAC TTGGATTATT AATCTGGGAA GGCCTTACCT 17351 .......... .......... .......... .......... .......... .......... 62 AGTTTGTACC CATGTCCCAT TTGTGACTAC TAATTTTGCA AGAAAAGGTT AAGTTCTATA 17411 .......... .......... .......... .......... .......... .......... 62 CATTATATCA GAGATACACA GACAATAGAC ATTAAATAAA TTACTTAAAA CTAATTAATG 17471 .......... .......... .......... .......... .......... .......... 62 TATTTAATTA ATTCCAAACA AGTTGTCATG CTTTACTAAA AAGATTGAAG GTTGAATATT 17531 .......... .......... .......... .......... .......... .......... 62 TTATCTGGAG AGATTGGGCA CTAATAAAAT GCACTTACCA TAACCATATC AGGGTAATTT 17591 .......... .......... .......... .......... .......... .......... 62 TTGATTTGGC ATGGAAATTT TCAGTTACTT AGATTTCTTG CAATTTCCTT TCTATTGACA 17651 .......... .......... .......... .......... .......... .......... 62 TAAATTTGAG AGGATGTTAA ATTCATCATG TAGTGTGTGC GATGGCACGC AAATTGCAAT 17711 ||||||| |||||||||| |||||||||| .......... .......... .......... ...TGTGTGC GATGGCACGC AAATTGCAAT 89 TACATTGCAA CCGGATCAAG CGACAAAACT GTACGCTTGT GGGACGTCCA GAGTGGTGAC 17771 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACATTGCAA CCGGATCAAG CGACAAAACT GTACGCTTGT GGGACGTCCA GAGTGGTGAC 149 TGTGTCAGAA TTTTCATTGG TCATAGGAGT ATGATCCTAT CATTGGCAAT GTCTCCTGAT 17831 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGTCAGAA TTTTCATTGG TCATAGGAGT ATGATCCTAT CATTGGCAAT GTCTCCTGAT 209 GGTCGATATA TGGCATCCGG TGATGAAGAT GGAACAGTCA TGATGTGGGA TCTCTCAAGT 17891 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTCGATATA TGGCATCCGG TGATGAAGAT GGAACAGTCA TGATGTGGGA TCTCTCAAGT 269 GGACGTTGTA TTACTCCTTT GACCGGTCAT TCCTCTTGTG TGTGGTCACT TGCTTTTAGG 17951 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GGACGTTGTA TTACTCCTTT GACCGGTCAT TCCTCTTGTG TGTGGTCACT TGCTTTTAG. 328 TAGGGATAGA AATGAACTTA ACCTTAAGTT TGTGTACGCT CAGAGATGCA CTTGAGTATG 18011 .......... .......... .......... .......... .......... .......... 328 AAGTTCATTG AATATAGATT ATATTTTTGA AGCTTTTTTG TCTTATTTCA GTGGTGAAGG 18071 ||||||||| .......... .......... .......... .......... .......... .TGGTGAAGG 337 TTCACTTCTT GCATCGGGCT CTGCGGATTG CACTGTAAAA TTATGGGATG TAACCACAAG 18131 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCACTTCTT GCATCGGGCT CTGCGGATTG CACTGTAAAA TTATGGGATG TAACCACAAG 397 CACCAGAGCG CCACTAAAAG AAGAGAAGTA AGTATTAATG AAGCAGACAT TTACTGTGGC 18191 |||||||||| |||||||||| ||||||| CACCAGAGCG CCACTAAAAG AAGAGAA... .......... .......... .......... 424 ATTTGTTTTC TCTTAGATTG CTGCTAATAC CAGCCCTCTC TCTCTCTATC TATTGAACAG 18251 .......... .......... .......... .......... .......... .......... 424 CAAATCTGGA GGTAACAATA GGCTTCGGTT ATTGAAGACC CTACCTACCA AAGCTACACC 18311 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAATCTGGA GGTAACAATA GGCTTCGGTT ATTGAAGACC CTACCTACCA AAGCTACACC 484 AATCTATGCT TTGCAC 18327 |||||||||| |||||| AATCTATGCT TTGCAC 500 hqPGS_C06HBa0046G10.1-4+_SGN-E203439+ (16572 16633,17685 17950,18063 18158,18252 18327) ******************************************************************************** EST sequence 18 +strand 475 n (File: SGN-E241154+) 1 CCTCTAAGAA TAATGGCCGG ACACTTGTCA GATGTAGATT GTGTGCGATG GCACGCAAAT 61 TGCAATTACA TTGCAACCGG ATCAAGCGAC AAAACTGTAC GCTTGTGGGA CGTCCAGAGT 121 GGTGACTGTG TCAGAATTTT CATTGGTCAT AGGAGTATGA TCCTATCATT GGCAATGTCT 181 CCTGATGGTC GATATATGGC ATCCGGTGAT GAAGATGGAA CAGTCATGAT GTGGGATCTC 241 TCAAGTGGAC GTTGTATTAC TCCTTTGACC GGTCATTCCT CTTGTGTGTG GTCACTTGCT 301 TTTAGTGGTG AAGGTTCACT TCTTGCATCG GGCTCTGCGG ATTGCACTGT AAAATTATGG 361 GATGTAACCA CAAGCACCAG AGCGCCACTA AAAGAAGAGA ACAAATCTGG AGGTAACAAT 421 AGGCTTCGGT TATTGAAGAC CCTACCTACC AAAGCTACAC CAATCTATGC TTTGC Predicted gene structure (within gDNA segment 15995 to 18935): Exon 1 16595 16633 ( 39 n); cDNA 1 39 ( 39 n); score: 1.000 Intron 1 16634 17684 (1051 n); Pd: 0.880 (s: 0), Pa: 0.958 (s: 1.00) Exon 2 17685 17950 ( 266 n); cDNA 40 305 ( 266 n); score: 1.000 Intron 2 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 3 18063 18158 ( 96 n); cDNA 306 401 ( 96 n); score: 1.000 Intron 3 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 4 18252 18325 ( 74 n); cDNA 402 475 ( 74 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E241154+ 1.000 475 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E241154+ (16595 16633,17685 17950,18063 18158,18252 18325) Alignment (genomic DNA sequence = upper lines): CCTCTAAGAA TAATGGCCGG ACACTTGTCA GATGTAGATG TGAGTATCTA GTTTGTGCTC 16654 |||||||||| |||||||||| |||||||||| ||||||||| CCTCTAAGAA TAATGGCCGG ACACTTGTCA GATGTAGAT. .......... .......... 39 TATGCCATGG GAACTATTTA TTTGATCTGA TCTACGTTGG CATCTTTATC ACTCTGTGGC 16714 .......... .......... .......... .......... .......... .......... 39 TGTATAATCA ATCTTAGTTA CTTGACCTAC TTTTCATTTT CGTCCTCTAT AATCACTTGG 16774 .......... .......... .......... .......... .......... .......... 39 CCTGTTATAA AGTGACATCT TTTCCCCATG TAAAAAAAAT GAAATGGCTA AGTTTTCCGA 16834 .......... .......... .......... .......... .......... .......... 39 GTGCTCAAAT ACCCAGACAT AGGGGGCCAT CTTTCCATTT TGTGAGGCCC CATAATGTTG 16894 .......... .......... .......... .......... .......... .......... 39 GCCACCACTG ATACTTTGGC AATTACAAGA AGTTAAGTTC ATTAAGTATT CTAATTTTTG 16954 .......... .......... .......... .......... .......... .......... 39 AACTTCTTGT GATGTTTTCA GGCGTACACT TAACTTTTCG ACTATCAATT TAGTTCTTTT 17014 .......... .......... .......... .......... .......... .......... 39 CTTAAACTAC TGCTAATACA TTTGATAAGT TCAAATGACA TCTTAAAGTC CATTCCAGTC 17074 .......... .......... .......... .......... .......... .......... 39 AAATATCTCA TATAAAATGG GACAGATGGA GAGTATAATG TTTCTTTTAG CTTTGATTTG 17134 .......... .......... .......... .......... .......... .......... 39 GTATCTTCTC AACAACAGTC CACACTTCAT TAACTTTTAC TTTTTAGTGT AACTCTTTCT 17194 .......... .......... .......... .......... .......... .......... 39 TTTGCCTTTT TTATCTTTTC ACCAACAATC CTCCCAAAAT AGTGATGTTT TTTGAAGGAT 17254 .......... .......... .......... .......... .......... .......... 39 CCGATTTGAT TGCGGCAATA ATTTTGGAGA GACTGAGCGC ATAGGGAAGA GTAACTTTTT 17314 .......... .......... .......... .......... .......... .......... 39 TTATAACTTG GATTATTAAT CTGGGAAGGC CTTACCTAGT TTGTACCCAT GTCCCATTTG 17374 .......... .......... .......... .......... .......... .......... 39 TGACTACTAA TTTTGCAAGA AAAGGTTAAG TTCTATACAT TATATCAGAG ATACACAGAC 17434 .......... .......... .......... .......... .......... .......... 39 AATAGACATT AAATAAATTA CTTAAAACTA ATTAATGTAT TTAATTAATT CCAAACAAGT 17494 .......... .......... .......... .......... .......... .......... 39 TGTCATGCTT TACTAAAAAG ATTGAAGGTT GAATATTTTA TCTGGAGAGA TTGGGCACTA 17554 .......... .......... .......... .......... .......... .......... 39 ATAAAATGCA CTTACCATAA CCATATCAGG GTAATTTTTG ATTTGGCATG GAAATTTTCA 17614 .......... .......... .......... .......... .......... .......... 39 GTTACTTAGA TTTCTTGCAA TTTCCTTTCT ATTGACATAA ATTTGAGAGG ATGTTAAATT 17674 .......... .......... .......... .......... .......... .......... 39 CATCATGTAG TGTGTGCGAT GGCACGCAAA TTGCAATTAC ATTGCAACCG GATCAAGCGA 17734 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... TGTGTGCGAT GGCACGCAAA TTGCAATTAC ATTGCAACCG GATCAAGCGA 89 CAAAACTGTA CGCTTGTGGG ACGTCCAGAG TGGTGACTGT GTCAGAATTT TCATTGGTCA 17794 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAAACTGTA CGCTTGTGGG ACGTCCAGAG TGGTGACTGT GTCAGAATTT TCATTGGTCA 149 TAGGAGTATG ATCCTATCAT TGGCAATGTC TCCTGATGGT CGATATATGG CATCCGGTGA 17854 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGGAGTATG ATCCTATCAT TGGCAATGTC TCCTGATGGT CGATATATGG CATCCGGTGA 209 TGAAGATGGA ACAGTCATGA TGTGGGATCT CTCAAGTGGA CGTTGTATTA CTCCTTTGAC 17914 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAAGATGGA ACAGTCATGA TGTGGGATCT CTCAAGTGGA CGTTGTATTA CTCCTTTGAC 269 CGGTCATTCC TCTTGTGTGT GGTCACTTGC TTTTAGGTAG GGATAGAAAT GAACTTAACC 17974 |||||||||| |||||||||| |||||||||| |||||| CGGTCATTCC TCTTGTGTGT GGTCACTTGC TTTTAG.... .......... .......... 305 TTAAGTTTGT GTACGCTCAG AGATGCACTT GAGTATGAAG TTCATTGAAT ATAGATTATA 18034 .......... .......... .......... .......... .......... .......... 305 TTTTTGAAGC TTTTTTGTCT TATTTCAGTG GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG 18094 || |||||||||| |||||||||| |||||||||| .......... .......... ........TG GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG 337 CGGATTGCAC TGTAAAATTA TGGGATGTAA CCACAAGCAC CAGAGCGCCA CTAAAAGAAG 18154 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGGATTGCAC TGTAAAATTA TGGGATGTAA CCACAAGCAC CAGAGCGCCA CTAAAAGAAG 397 AGAAGTAAGT ATTAATGAAG CAGACATTTA CTGTGGCATT TGTTTTCTCT TAGATTGCTG 18214 |||| AGAA...... .......... .......... .......... .......... .......... 401 CTAATACCAG CCCTCTCTCT CTCTATCTAT TGAACAGCAA ATCTGGAGGT AACAATAGGC 18274 ||| |||||||||| |||||||||| .......... .......... .......... .......CAA ATCTGGAGGT AACAATAGGC 424 TTCGGTTATT GAAGACCCTA CCTACCAAAG CTACACCAAT CTATGCTTTG C 18325 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | TTCGGTTATT GAAGACCCTA CCTACCAAAG CTACACCAAT CTATGCTTTG C 475 hqPGS_C06HBa0046G10.1-4+_SGN-E241154+ (16595 16633,17685 17950,18063 18158,18252 18325) ******************************************************************************** EST sequence 6 +strand 563 n (File: SGN-E253821+) 1 ATTGCAATTA CATTGCAACC GGATCAAGCG ACAAAACTGT ACGCTTGTGG GACGTCCAGA 61 GTGGTGACTG TGTCAGAATT TTCATTGGTC ATAGGAGTAT GATCCTATCA TTGGCAATGT 121 CTCCTGATGG TCGATATATG GCATCCGGTG ATGAAGATGG AACAGTCATG ATGTGGGATC 181 TCTCAAGTGG ACGTTGTATT ACTCCTTTGA CCGGTCATTC CTCTTGTGTG TGGTCACTTG 241 CTTTTAGTGG TGAAGGTTCA CTTCTTGCAT CGGGCTCTGC GGATTGCACT GTAAAATTAT 301 GGGATGTAAC CACAAGCACC AGAGCGCCAC TAAAAGAAGA GAACAAATCT GGAGGTAACA 361 ATAGGCTTCG GTTATTGAAG ACCCTACCTA CCAAAGCTAC ACCAATCTAT GCTTTGCACT 421 TTTCACGAAG AAATCTTTTA TTTGCTGCTG GAGCTTTCTC GAAAAGTGCA CGAGTCCCTT 481 GCTTTAAAGT CTCTTGACGT GAGATTACAT CATGGTTCCC TTGCAAATTT TGGTGCAATC 541 ATCAATGGCA AGTTTTACTT CTC Predicted gene structure (within gDNA segment 17104 to 19161): Exon 1 17704 17950 ( 247 n); cDNA 1 247 ( 247 n); score: 1.000 Intron 1 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 18063 18158 ( 96 n); cDNA 248 343 ( 96 n); score: 1.000 Intron 2 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 18252 18327 ( 76 n); cDNA 344 419 ( 76 n); score: 1.000 Intron 3 18328 18407 ( 80 n); Pd: 0.975 (s: 1.00), Pa: 0.489 (s: 1.00) Exon 4 18408 18551 ( 144 n); cDNA 420 563 ( 144 n); score: 1.000 MATCH C06HBa0046G10.1-4+ SGN-E253821+ 1.000 563 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E253821+ (17704 17950,18063 18158,18252 18327,18408 18551) Alignment (genomic DNA sequence = upper lines): ATTGCAATTA CATTGCAACC GGATCAAGCG ACAAAACTGT ACGCTTGTGG GACGTCCAGA 17763 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGCAATTA CATTGCAACC GGATCAAGCG ACAAAACTGT ACGCTTGTGG GACGTCCAGA 60 GTGGTGACTG TGTCAGAATT TTCATTGGTC ATAGGAGTAT GATCCTATCA TTGGCAATGT 17823 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGGTGACTG TGTCAGAATT TTCATTGGTC ATAGGAGTAT GATCCTATCA TTGGCAATGT 120 CTCCTGATGG TCGATATATG GCATCCGGTG ATGAAGATGG AACAGTCATG ATGTGGGATC 17883 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCTGATGG TCGATATATG GCATCCGGTG ATGAAGATGG AACAGTCATG ATGTGGGATC 180 TCTCAAGTGG ACGTTGTATT ACTCCTTTGA CCGGTCATTC CTCTTGTGTG TGGTCACTTG 17943 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCAAGTGG ACGTTGTATT ACTCCTTTGA CCGGTCATTC CTCTTGTGTG TGGTCACTTG 240 CTTTTAGGTA GGGATAGAAA TGAACTTAAC CTTAAGTTTG TGTACGCTCA GAGATGCACT 18003 ||||||| CTTTTAG... .......... .......... .......... .......... .......... 247 TGAGTATGAA GTTCATTGAA TATAGATTAT ATTTTTGAAG CTTTTTTGTC TTATTTCAGT 18063 | .......... .......... .......... .......... .......... .........T 248 GGTGAAGGTT CACTTCTTGC ATCGGGCTCT GCGGATTGCA CTGTAAAATT ATGGGATGTA 18123 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTGAAGGTT CACTTCTTGC ATCGGGCTCT GCGGATTGCA CTGTAAAATT ATGGGATGTA 308 ACCACAAGCA CCAGAGCGCC ACTAAAAGAA GAGAAGTAAG TATTAATGAA GCAGACATTT 18183 |||||||||| |||||||||| |||||||||| ||||| ACCACAAGCA CCAGAGCGCC ACTAAAAGAA GAGAA..... .......... .......... 343 ACTGTGGCAT TTGTTTTCTC TTAGATTGCT GCTAATACCA GCCCTCTCTC TCTCTATCTA 18243 .......... .......... .......... .......... .......... .......... 343 TTGAACAGCA AATCTGGAGG TAACAATAGG CTTCGGTTAT TGAAGACCCT ACCTACCAAA 18303 || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ........CA AATCTGGAGG TAACAATAGG CTTCGGTTAT TGAAGACCCT ACCTACCAAA 395 GCTACACCAA TCTATGCTTT GCACGTAAGA ATTGTTATGC TTGCTTCTTT CTGTGGCTTT 18363 |||||||||| |||||||||| |||| GCTACACCAA TCTATGCTTT GCAC...... .......... .......... .......... 419 ATTATTGGAT CCTCTAAAAG TTGCATGTTA TACTCTGTGA GCAGTTTTCA CGAAGAAATC 18423 |||||| |||||||||| .......... .......... .......... .......... ....TTTTCA CGAAGAAATC 435 TTTTATTTGC TGCTGGAGCT TTCTCGAAAA GTGCACGAGT CCCTTGCTTT AAAGTCTCTT 18483 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTATTTGC TGCTGGAGCT TTCTCGAAAA GTGCACGAGT CCCTTGCTTT AAAGTCTCTT 495 GACGTGAGAT TACATCATGG TTCCCTTGCA AATTTTGGTG CAATCATCAA TGGCAAGTTT 18543 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACGTGAGAT TACATCATGG TTCCCTTGCA AATTTTGGTG CAATCATCAA TGGCAAGTTT 555 TACTTCTC 18551 |||||||| TACTTCTC 563 hqPGS_C06HBa0046G10.1-4+_SGN-E253821+ (17704 17950,18063 18158,18252 18327,18408 18551) ******************************************************************************** EST sequence 16 +strand 554 n (File: SGN-E244420+) 1 ATAGGAGTAT GATCCTATCA TTGGCAATGT CTCCTGATGG TCGATATATG GCATCCGGTG 61 ATGAAGATGG AACAGTCATG ATGTGGGATC TCTCAAGTGG ACGTTGTATT ACTCCTTTGA 121 CCGGTCATTC CTCTTGTGTG TGGTCACTTG CTTTTAGTGG TGAAGGTTCA CTTCTTGCAT 181 CGGGCTCTGC GGATTGCACT GTAAAATTAT GGGATGTAAC CACAAGCACC AGAGCGCCAC 241 TAAAAGAAGA GAACAAATCT GGAGGTAACA ATAGGCTTCG GTTATTGAAG ACCCTACCTA 301 CCAAAGCTAC ACCAATCTAT GCTTTGCACT TTTCACGAAG AAATCTTTTA TTTGCTGCTG 361 GAGCTTTCTC GAAAAGTGCA CGAGTCCCTT GCTTTAAAGT CTCTTGACGT GAGATTACAT 421 CATGGTTCCC TTGCAAATTT TGGTGCAATC ATCAATGGCA AGTTTTACTT CTCAGACCTC 481 GGTTTTGTTA GAGGAGCAAC TCATGTTGGT GACACGTGCA TCTTACAATA CAATTTTGCA 541 TCACATGGTG TAAT Predicted gene structure (within gDNA segment 17194 to 19242): Exon 1 17794 17950 ( 157 n); cDNA 1 157 ( 157 n); score: 1.000 Intron 1 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 18063 18158 ( 96 n); cDNA 158 253 ( 96 n); score: 1.000 Intron 2 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 18252 18327 ( 76 n); cDNA 254 329 ( 76 n); score: 1.000 Intron 3 18328 18407 ( 80 n); Pd: 0.975 (s: 1.00), Pa: 0.489 (s: 1.00) Exon 4 18408 18632 ( 225 n); cDNA 330 554 ( 225 n); score: 0.996 MATCH C06HBa0046G10.1-4+ SGN-E244420+ 0.998 554 1.000 C PGS_C06HBa0046G10.1-4+_SGN-E244420+ (17794 17950,18063 18158,18252 18327,18408 18632) Alignment (genomic DNA sequence = upper lines): ATAGGAGTAT GATCCTATCA TTGGCAATGT CTCCTGATGG TCGATATATG GCATCCGGTG 17853 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAGGAGTAT GATCCTATCA TTGGCAATGT CTCCTGATGG TCGATATATG GCATCCGGTG 60 ATGAAGATGG AACAGTCATG ATGTGGGATC TCTCAAGTGG ACGTTGTATT ACTCCTTTGA 17913 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAAGATGG AACAGTCATG ATGTGGGATC TCTCAAGTGG ACGTTGTATT ACTCCTTTGA 120 CCGGTCATTC CTCTTGTGTG TGGTCACTTG CTTTTAGGTA GGGATAGAAA TGAACTTAAC 17973 |||||||||| |||||||||| |||||||||| ||||||| CCGGTCATTC CTCTTGTGTG TGGTCACTTG CTTTTAG... .......... .......... 157 CTTAAGTTTG TGTACGCTCA GAGATGCACT TGAGTATGAA GTTCATTGAA TATAGATTAT 18033 .......... .......... .......... .......... .......... .......... 157 ATTTTTGAAG CTTTTTTGTC TTATTTCAGT GGTGAAGGTT CACTTCTTGC ATCGGGCTCT 18093 | |||||||||| |||||||||| |||||||||| .......... .......... .........T GGTGAAGGTT CACTTCTTGC ATCGGGCTCT 188 GCGGATTGCA CTGTAAAATT ATGGGATGTA ACCACAAGCA CCAGAGCGCC ACTAAAAGAA 18153 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCGGATTGCA CTGTAAAATT ATGGGATGTA ACCACAAGCA CCAGAGCGCC ACTAAAAGAA 248 GAGAAGTAAG TATTAATGAA GCAGACATTT ACTGTGGCAT TTGTTTTCTC TTAGATTGCT 18213 ||||| GAGAA..... .......... .......... .......... .......... .......... 253 GCTAATACCA GCCCTCTCTC TCTCTATCTA TTGAACAGCA AATCTGGAGG TAACAATAGG 18273 || |||||||||| |||||||||| .......... .......... .......... ........CA AATCTGGAGG TAACAATAGG 275 CTTCGGTTAT TGAAGACCCT ACCTACCAAA GCTACACCAA TCTATGCTTT GCACGTAAGA 18333 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| CTTCGGTTAT TGAAGACCCT ACCTACCAAA GCTACACCAA TCTATGCTTT GCAC...... 329 ATTGTTATGC TTGCTTCTTT CTGTGGCTTT ATTATTGGAT CCTCTAAAAG TTGCATGTTA 18393 .......... .......... .......... .......... .......... .......... 329 TACTCTGTGA GCAGTTTTCA CGAAGAAATC TTTTATTTGC TGCTGGAGCT TTCTCGAAAA 18453 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....TTTTCA CGAAGAAATC TTTTATTTGC TGCTGGAGCT TTCTCGAAAA 375 GTGCACGAGT CCCTTGCTTT AAAGTCTCTT GACGTGAGAT TACATCATGG TTCCCTTGCA 18513 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGCACGAGT CCCTTGCTTT AAAGTCTCTT GACGTGAGAT TACATCATGG TTCCCTTGCA 435 AATTTTGGTG CAATCATCAA TGGCAAGTTT TACTTCTCAG ACCTCGGTTT TGTTAGAGGA 18573 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATTTTGGTG CAATCATCAA TGGCAAGTTT TACTTCTCAG ACCTCGGTTT TGTTAGAGGA 495 GCAACTCATG TTGGTGACAC GTGCATCTCA CAATACAATT TTGCATCACA TGGTGTAAT 18632 |||||||||| |||||||||| |||||||| | |||||||||| |||||||||| ||||||||| GCAACTCATG TTGGTGACAC GTGCATCTTA CAATACAATT TTGCATCACA TGGTGTAAT 554 hqPGS_C06HBa0046G10.1-4+_SGN-E244420+ (17794 17950,18063 18158,18252 18327,18408 18632) ******************************************************************************** EST sequence 15 +strand 554 n (File: SGN-E256654+) 1 TTTTTTTTTT TTTTTTTTGA GGAAAGGGTA TCATAACATT ATCAAGTAAC AACACTTCGA 61 CAAAGGGTAT GAGAAACATT ACCAAGTATA CCAACTTGGG CAAAAAGCAA AATGAAAAAC 121 TATTAGAAAA AAGACCATGG CCGTTTACCA GCCACCTCCA CCTCTTCTGT CTTGCCTCAA 181 GTGGACGTTG TATTACTCCT TTGACCGGTC ATTCCTCTTG TGTGTGGTCA CTTGCTTTTA 241 GTGGTGAAGG TTCACTTCTT GCATCGGGCT CTGCGGATTG CACTGTAAAA TTATGGGATG 301 TAACCACAAG CACCAGAGCG CCACTAAAAG AAGAGAACAA ATCTGGAGGT AACAATAGGC 361 TTCGGTTATT GAAGACCCTA CCTACCAAAG CTACACCAAT CTATGCTTTG CACTTTTCAC 421 GAAGAAATCT TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA 481 AAGTCTCTTG ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT 541 GGCAAGTTTT ACTT Predicted gene structure (within gDNA segment 15535 to 19158): Exon 1 17885 17950 ( 66 n); cDNA 176 241 ( 66 n); score: 1.000 Intron 1 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 18063 18158 ( 96 n); cDNA 242 337 ( 96 n); score: 1.000 Intron 2 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 18252 18327 ( 76 n); cDNA 338 413 ( 76 n); score: 1.000 Intron 3 18328 18407 ( 80 n); Pd: 0.975 (s: 1.00), Pa: 0.489 (s: 1.00) Exon 4 18408 18548 ( 141 n); cDNA 414 554 ( 141 n); score: 1.000 PPA cDNA 18 1 MATCH C06HBa0046G10.1-4+ SGN-E256654+ 1.000 379 0.684 C PGS_C06HBa0046G10.1-4+_SGN-E256654+ (17885 17950,18063 18158,18252 18327,18408 18548) Alignment (genomic DNA sequence = upper lines): CTCAAGTGGA CGTTGTATTA CTCCTTTGAC CGGTCATTCC TCTTGTGTGT GGTCACTTGC 17944 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAAGTGGA CGTTGTATTA CTCCTTTGAC CGGTCATTCC TCTTGTGTGT GGTCACTTGC 235 TTTTAGGTAG GGATAGAAAT GAACTTAACC TTAAGTTTGT GTACGCTCAG AGATGCACTT 18004 |||||| TTTTAG.... .......... .......... .......... .......... .......... 241 GAGTATGAAG TTCATTGAAT ATAGATTATA TTTTTGAAGC TTTTTTGTCT TATTTCAGTG 18064 || .......... .......... .......... .......... .......... ........TG 243 GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG CGGATTGCAC TGTAAAATTA TGGGATGTAA 18124 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG CGGATTGCAC TGTAAAATTA TGGGATGTAA 303 CCACAAGCAC CAGAGCGCCA CTAAAAGAAG AGAAGTAAGT ATTAATGAAG CAGACATTTA 18184 |||||||||| |||||||||| |||||||||| |||| CCACAAGCAC CAGAGCGCCA CTAAAAGAAG AGAA...... .......... .......... 337 CTGTGGCATT TGTTTTCTCT TAGATTGCTG CTAATACCAG CCCTCTCTCT CTCTATCTAT 18244 .......... .......... .......... .......... .......... .......... 337 TGAACAGCAA ATCTGGAGGT AACAATAGGC TTCGGTTATT GAAGACCCTA CCTACCAAAG 18304 ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......CAA ATCTGGAGGT AACAATAGGC TTCGGTTATT GAAGACCCTA CCTACCAAAG 390 CTACACCAAT CTATGCTTTG CACGTAAGAA TTGTTATGCT TGCTTCTTTC TGTGGCTTTA 18364 |||||||||| |||||||||| ||| CTACACCAAT CTATGCTTTG CAC....... .......... .......... .......... 413 TTATTGGATC CTCTAAAAGT TGCATGTTAT ACTCTGTGAG CAGTTTTCAC GAAGAAATCT 18424 ||||||| |||||||||| .......... .......... .......... .......... ...TTTTCAC GAAGAAATCT 430 TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA AAGTCTCTTG 18484 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA AAGTCTCTTG 490 ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT GGCAAGTTTT 18544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT GGCAAGTTTT 550 ACTT 18548 |||| ACTT 554 hqPGS_C06HBa0046G10.1-4+_SGN-E256654+ (17885 17950,18063 18158,18252 18327,18408 18548) ******************************************************************************** EST sequence 8 +strand 552 n (File: SGN-E255083+) 1 TTTTTTTTTT TTTTTTTTGA GGAAAGGGTA TCATAGGGTT ATGAAGTAAC AACACTTCGA 61 CAAAGGGTAT GAGAAACATT ACCAAGTATA CCAACTTGGG CAAAAAGCAA AATGAAAAAC 121 TATTAGAAAA AAGACCATGG CCGTTTACCA GCCACCTCCA CCTCTTCTGT CTTGCCTCAA 181 GTGGACGTTG TATTACTCCT TTGACCGGTC ATTCCTCTTG TGTGTGGTCA CTTGCTTTTA 241 GTGGTGAAGG TTCACTTCTT GCATCGGGCT CTGCGGATTG CACTGTAAAA TTATGGGATG 301 TAACCACAAG CACCAGAGCG CCACTAAAAG AAGAGAACAA ATCTGGAGGT AACAATAGGC 361 TTCGGTTATT GAAGACCCTA CCTACCAAAG CTACACCAAT CTATGCTTTG CACTTTTCAC 421 GAAGAAATCT TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA 481 AAGTCTCTTG ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT 541 GGCAAGTTTT AC Predicted gene structure (within gDNA segment 15535 to 19156): Exon 1 17885 17950 ( 66 n); cDNA 176 241 ( 66 n); score: 1.000 Intron 1 17951 18062 ( 112 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 18063 18158 ( 96 n); cDNA 242 337 ( 96 n); score: 1.000 Intron 2 18159 18251 ( 93 n); Pd: 0.960 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 18252 18327 ( 76 n); cDNA 338 413 ( 76 n); score: 1.000 Intron 3 18328 18407 ( 80 n); Pd: 0.975 (s: 1.00), Pa: 0.489 (s: 1.00) Exon 4 18408 18546 ( 139 n); cDNA 414 552 ( 139 n); score: 1.000 PPA cDNA 18 1 MATCH C06HBa0046G10.1-4+ SGN-E255083+ 1.000 377 0.683 C PGS_C06HBa0046G10.1-4+_SGN-E255083+ (17885 17950,18063 18158,18252 18327,18408 18546) Alignment (genomic DNA sequence = upper lines): CTCAAGTGGA CGTTGTATTA CTCCTTTGAC CGGTCATTCC TCTTGTGTGT GGTCACTTGC 17944 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAAGTGGA CGTTGTATTA CTCCTTTGAC CGGTCATTCC TCTTGTGTGT GGTCACTTGC 235 TTTTAGGTAG GGATAGAAAT GAACTTAACC TTAAGTTTGT GTACGCTCAG AGATGCACTT 18004 |||||| TTTTAG.... .......... .......... .......... .......... .......... 241 GAGTATGAAG TTCATTGAAT ATAGATTATA TTTTTGAAGC TTTTTTGTCT TATTTCAGTG 18064 || .......... .......... .......... .......... .......... ........TG 243 GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG CGGATTGCAC TGTAAAATTA TGGGATGTAA 18124 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGAAGGTTC ACTTCTTGCA TCGGGCTCTG CGGATTGCAC TGTAAAATTA TGGGATGTAA 303 CCACAAGCAC CAGAGCGCCA CTAAAAGAAG AGAAGTAAGT ATTAATGAAG CAGACATTTA 18184 |||||||||| |||||||||| |||||||||| |||| CCACAAGCAC CAGAGCGCCA CTAAAAGAAG AGAA...... .......... .......... 337 CTGTGGCATT TGTTTTCTCT TAGATTGCTG CTAATACCAG CCCTCTCTCT CTCTATCTAT 18244 .......... .......... .......... .......... .......... .......... 337 TGAACAGCAA ATCTGGAGGT AACAATAGGC TTCGGTTATT GAAGACCCTA CCTACCAAAG 18304 ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......CAA ATCTGGAGGT AACAATAGGC TTCGGTTATT GAAGACCCTA CCTACCAAAG 390 CTACACCAAT CTATGCTTTG CACGTAAGAA TTGTTATGCT TGCTTCTTTC TGTGGCTTTA 18364 |||||||||| |||||||||| ||| CTACACCAAT CTATGCTTTG CAC....... .......... .......... .......... 413 TTATTGGATC CTCTAAAAGT TGCATGTTAT ACTCTGTGAG CAGTTTTCAC GAAGAAATCT 18424 ||||||| |||||||||| .......... .......... .......... .......... ...TTTTCAC GAAGAAATCT 430 TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA AAGTCTCTTG 18484 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTATTTGCT GCTGGAGCTT TCTCGAAAAG TGCACGAGTC CCTTGCTTTA AAGTCTCTTG 490 ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT GGCAAGTTTT 18544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTGAGATT ACATCATGGT TCCCTTGCAA ATTTTGGTGC AATCATCAAT GGCAAGTTTT 550 AC 18546 || AC 552 hqPGS_C06HBa0046G10.1-4+_SGN-E255083+ (17885 17950,18063 18158,18252 18327,18408 18546) ******************************************************************************** EST sequence 25 +strand 530 n (File: SGN-E286841+) 1 GAGCAAGTAG CCAGCGCTGA TGCAGCAGCT TCGGAAAGTC ATAAGAAGAA AAAACCTAGG 61 AATGCTGAAC CATCACTAAA GCATGAAATG GATGCTTCAC CTAAAAGCAA GTCAAAAGAG 121 GCGACAGCTA AATCTGGACA GAAATCCAAG GGTAAACTCA ACTTGAAAGA TGGCACCTCA 181 AAATCTGCCG GGAAAGCTGA TGACACAACC AGCAGCAAGT CTGCGGCTCC ATCCAAAAGG 241 AGCACTGGCA AATCAGTTGA TACTGAAAAG CCATCTGCCA GGTCTAAAGA TGTTAGTAGT 301 AGTACTCCCA AATCCAAGTC CAGGCAAGAT ACTCCATCAA CAACTGCCAA CAAGTCCAAG 361 CAAGAAACAG TTAAAGCTGC CAACAAGTCC AAAACCAAAA CCCCCCAAAG TGGTGGCAAG 421 TCCGGTGCCA ATGGTTCGGA GAAGCTAAAA TCTAGTTCAT CAAAGGTTAA AGAGAGTGGG 481 AACCAGAAGG AAAAAGCAAC TAACTCAGCA AAAACCCCTG ATGGCAGCAC Predicted gene structure (within gDNA segment 22433 to 19931): Exon 1 21833 21796 ( 38 n); cDNA 1 38 ( 38 n); score: 1.000 Intron 1 21795 21128 ( 668 n); Pd: 0.964 (s: 0), Pa: 0.999 (s: 1.00) Exon 2 21127 21059 ( 69 n); cDNA 39 107 ( 69 n); score: 1.000 Intron 2 21058 20964 ( 95 n); Pd: 0.996 (s: 1.00), Pa: 0.972 (s: 1.00) Exon 3 20963 20541 ( 423 n); cDNA 108 530 ( 423 n); score: 1.000 MATCH C06HBa0046G10.1-4- SGN-E286841+ 1.000 530 1.000 C PGS_C06HBa0046G10.1-4-_SGN-E286841+ (21833 21796,21127 21059,20963 20541) Alignment (genomic DNA sequence = upper lines): GAGCAAGTAG CCAGCGCTGA TGCAGCAGCT TCGGAAAGGT AAACCTGGCC ATTAGTCACC 21774 |||||||||| |||||||||| |||||||||| |||||||| GAGCAAGTAG CCAGCGCTGA TGCAGCAGCT TCGGAAAG.. .......... .......... 38 ATTTTCTTTA TTTGGTTTTG ACTCATTTAT TTTCACCTGA AGATGCTTAC TCGATTTGAG 21714 .......... .......... .......... .......... .......... .......... 38 CCAAGTGATC TGAATTGTCT CCCTTTGTGA AACCCTTTTG CATTTTTTCA TCTACAAGTG 21654 .......... .......... .......... .......... .......... .......... 38 TCTGTTTAGC ATTATAACCA CTTGCTAGTA TGTCTCAACT GAAGAAACAA CACAAGATTC 21594 .......... .......... .......... .......... .......... .......... 38 TTGTTGCTGT GTTGTGCTAT TTTTGTGTAC CTTTGATGTT TTCTGGGTTT GATATCTTTG 21534 .......... .......... .......... .......... .......... .......... 38 TCCAGTCTAT AGTTTCATTT TTTTTGTGAT GCATGCGATG CTATCATAAG CAAAAAAAGC 21474 .......... .......... .......... .......... .......... .......... 38 AATTTGTCGA TTTCCTTTTT CTGTTTCCAA ATATTCTGCT AACTGTAGGA GAAGTGGTAA 21414 .......... .......... .......... .......... .......... .......... 38 GTACGTTGAT ATGAGTGATA GTGATTTCCT TTATTACATG GAAATTCTTC TTCGATGAGC 21354 .......... .......... .......... .......... .......... .......... 38 AAAGTGAAAC CAATACTCAA TTTGATGTTT GTACTCAATT GTGTTATTAG ATTATAATAT 21294 .......... .......... .......... .......... .......... .......... 38 TTTTGTAGCT GCGATTTACT CTCTTTTCTT AATCTCTCTG TGTAGAAGTA CTTTTAACAT 21234 .......... .......... .......... .......... .......... .......... 38 TCATTCTATT CATTGTGTTA GGTAAAGAAA GGACAGAAAT CTTCCAAGTG TAGTTAGCAG 21174 .......... .......... .......... .......... .......... .......... 38 TATGACTATT CTTTGTTTAA CTTTGACTCT GTTTTTGATA TGTCAGTCAT AAGAAGAAAA 21114 |||| |||||||||| .......... .......... .......... .......... ......TCAT AAGAAGAAAA 52 AACCTAGGAA TGCTGAACCA TCACTAAAGC ATGAAATGGA TGCTTCACCT AAAAGGTTGG 21054 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| AACCTAGGAA TGCTGAACCA TCACTAAAGC ATGAAATGGA TGCTTCACCT AAAAG..... 107 TATAATCTCC TTAACCATGT GTTTGTGTGA CATATTAACA TCTGACGTTT TGAAAGCCTA 20994 .......... .......... .......... .......... .......... .......... 107 ATGGTACCAA TGGTGGGGTT CTTTTTGCAG CAAGTCAAAA GAGGCGACAG CTAAATCTGG 20934 |||||||||| |||||||||| |||||||||| .......... .......... .......... CAAGTCAAAA GAGGCGACAG CTAAATCTGG 137 ACAGAAATCC AAGGGTAAAC TCAACTTGAA AGATGGCACC TCAAAATCTG CCGGGAAAGC 20874 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACAGAAATCC AAGGGTAAAC TCAACTTGAA AGATGGCACC TCAAAATCTG CCGGGAAAGC 197 TGATGACACA ACCAGCAGCA AGTCTGCGGC TCCATCCAAA AGGAGCACTG GCAAATCAGT 20814 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATGACACA ACCAGCAGCA AGTCTGCGGC TCCATCCAAA AGGAGCACTG GCAAATCAGT 257 TGATACTGAA AAGCCATCTG CCAGGTCTAA AGATGTTAGT AGTAGTACTC CCAAATCCAA 20754 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATACTGAA AAGCCATCTG CCAGGTCTAA AGATGTTAGT AGTAGTACTC CCAAATCCAA 317 GTCCAGGCAA GATACTCCAT CAACAACTGC CAACAAGTCC AAGCAAGAAA CAGTTAAAGC 20694 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCCAGGCAA GATACTCCAT CAACAACTGC CAACAAGTCC AAGCAAGAAA CAGTTAAAGC 377 TGCCAACAAG TCCAAAACCA AAACCCCCCA AAGTGGTGGC AAGTCCGGTG CCAATGGTTC 20634 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCCAACAAG TCCAAAACCA AAACCCCCCA AAGTGGTGGC AAGTCCGGTG CCAATGGTTC 437 GGAGAAGCTA AAATCTAGTT CATCAAAGGT TAAAGAGAGT GGGAACCAGA AGGAAAAAGC 20574 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAGAAGCTA AAATCTAGTT CATCAAAGGT TAAAGAGAGT GGGAACCAGA AGGAAAAAGC 497 AACTAACTCA GCAAAAACCC CTGATGGCAG CAC 20541 |||||||||| |||||||||| |||||||||| ||| AACTAACTCA GCAAAAACCC CTGATGGCAG CAC 530 hqPGS_C06HBa0046G10.1-4-_SGN-E286841+ (21833 21796,21127 21059,20963 20541) ******************************************************************************** EST sequence 11 -strand 182 n (File: SGN-E226670-) 1 GTTTTTTTTT TCAGTTTAAA GCATATTGTT AGAGATGTAA TTGGTTAGAT TTATCATTTT 61 GGTTGCTAGC TGTATTCACT TTTGTTACTT TTAGCCCTCT TAGCGTAGGC AGTAGATACT 121 CTGTTGATGA GTATTTTATG GATGCGTTGG ATCATCAATT ACCATCTTTT CTCTTGAAAA 181 AA Predicted gene structure (within gDNA segment 20878 to 19244): Exon 1 20268 20093 ( 176 n); cDNA 1 176 ( 176 n); score: 0.977 MATCH C06HBa0046G10.1-4- SGN-E226670- 0.977 176 0.967 C PGS_C06HBa0046G10.1-4-_SGN-E226670- (20268 20093) Alignment (genomic DNA sequence = upper lines): GTTTTTTTTT TCAGTTTAAA GCATATTGTT AGAGATGTAA TTGGTTAGAT TTATCATTTT 20209 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTTTTTT TCAGTTTAAA GCATATTGTT AGAGATGTAA TTGGTTAGAT TTATCATTTT 60 GGTTGCTAGC TGTATTCACT TTTGTTACTT TTAGCCCTCT TAGCGTAGGA AGGAGATACT 20149 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| || ||||||| GGTTGCTAGC TGTATTCACT TTTGTTACTT TTAGCCCTCT TAGCGTAGGC AGTAGATACT 120 CTGTTGATGA GTATTTTATG GATGCGTTGG ATCATAAATT ACCATCTCTT CTCTTG 20093 |||||||||| |||||||||| |||||||||| ||||| |||| ||||||| || |||||| CTGTTGATGA GTATTTTATG GATGCGTTGG ATCATCAATT ACCATCTTTT CTCTTG 176 hqPGS_C06HBa0046G10.1-4-_SGN-E226670- (20268 20093) ******************************************************************************** EST sequence 20 +strand 544 n (File: SGN-E325374+) 1 CTGATCGCTC TGTTAAACAT GATGAAGATA ACGAAGAGGA AATTCCCAGG ACCACTACAA 61 AGAGAAAGCG TTCTTCAAGC AAGGGCAGGG GGAGTAGGCA AGTTGTGCAG AAGAGTGTGC 121 CTACGCCCGA GTCTCCCGAC AATTCAACTA AACATATAAG CGACGAAGAT GAGACTGACA 181 CAAGTGCAAA GAAAAAGCCT TCTTCAGGCA ACGACAGGGT AACAGAGACT GTGCAATGTG 241 ATAAGAATCT GGTTGGTAGA AAGATCAGAG TTTGGTGGCC ATTAGATGAA CTATTTTATG 301 AAGGGACGGT TAGCAATTAT GATTCTTCCA GGAAGAAGTT CACAGTTGAC TATACTGATG 361 GAGAGACAGA AAAATTGAAT CTCTTGAAGG AACGCTGGGA ACTTGTTGAA GATGATAATA 421 TGTCAGAAGA GGAGCAAGTA GCCAGCGCTG ATGCAGCAGC TTCGGAAAGT CATAAGAAGA 481 AAAAACCTAG GAATGCTGAA CCATCACTAA AGCATGAAAT GGATGCTTCA CCTAAAAGCA 541 AGTC Predicted gene structure (within gDNA segment 23814 to 20389): Exon 1 23214 23126 ( 89 n); cDNA 1 89 ( 89 n); score: 1.000 Intron 1 23125 22954 ( 172 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 22953 22825 ( 129 n); cDNA 90 218 ( 129 n); score: 1.000 Intron 2 22824 22365 ( 460 n); Pd: 1.000 (s: 1.00), Pa: 0.678 (s: 1.00) Exon 3 22364 22291 ( 74 n); cDNA 219 292 ( 74 n); score: 1.000 Intron 3 22290 22132 ( 159 n); Pd: 0.984 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 4 22131 22080 ( 52 n); cDNA 293 344 ( 52 n); score: 1.000 Intron 4 22079 21993 ( 87 n); Pd: 0.583 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 5 21992 21906 ( 87 n); cDNA 345 431 ( 87 n); score: 1.000 Intron 5 21905 21834 ( 72 n); Pd: 1.000 (s: 1.00), Pa: 0.996 (s: 0) Exon 6 21833 21796 ( 38 n); cDNA 432 469 ( 38 n); score: 1.000 Intron 6 21795 21128 ( 668 n); Pd: 0.964 (s: 0), Pa: 0.999 (s: 1.00) Exon 7 21127 21059 ( 69 n); cDNA 470 538 ( 69 n); score: 1.000 Intron 7 21058 20964 ( 95 n); Pd: 0.996 (s: 1.00), Pa: 0.972 (s: 0) Exon 8 20963 20958 ( 6 n); cDNA 539 544 ( 6 n); score: 1.000 MATCH C06HBa0046G10.1-4- SGN-E325374+ 1.000 544 1.000 C PGS_C06HBa0046G10.1-4-_SGN-E325374+ (23214 23126,22953 22825,22364 22291,22131 22080,21992 21906,21833 21796,21127 21059,20963 20958) Alignment (genomic DNA sequence = upper lines): CTGATCGCTC TGTTAAACAT GATGAAGATA ACGAAGAGGA AATTCCCAGG ACCACTACAA 23155 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGATCGCTC TGTTAAACAT GATGAAGATA ACGAAGAGGA AATTCCCAGG ACCACTACAA 60 AGAGAAAGCG TTCTTCAAGC AAGGGCAGGG TAAGCATTTT ACATCATTGA TTTCATGTAT 23095 |||||||||| |||||||||| ||||||||| AGAGAAAGCG TTCTTCAAGC AAGGGCAGG. .......... .......... .......... 89 AATGCACATT TTATTCGAGG TAGTATTTTG ATGTGCTATT TTTTCCTGTT TCTGTAATTC 23035 .......... .......... .......... .......... .......... .......... 89 TTATGACTGA GCTTGTTCAA TTTTACTTAC TTACCATGAC CCCTCCGTGT CTGTCTCATC 22975 .......... .......... .......... .......... .......... .......... 89 AGTTGTTATT TCGTTGTGCA GGGGAGTAGG CAAGTTGTGC AGAAGAGTGT GCCTACGCCC 22915 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .GGGAGTAGG CAAGTTGTGC AGAAGAGTGT GCCTACGCCC 128 GAGTCTCCCG ACAATTCAAC TAAACATATA AGCGACGAAG ATGAGACTGA CACAAGTGCA 22855 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGTCTCCCG ACAATTCAAC TAAACATATA AGCGACGAAG ATGAGACTGA CACAAGTGCA 188 AAGAAAAAGC CTTCTTCAGG CAACGACAGG GTAAGCATTT CAAATTACTG TTCATTTTTG 22795 |||||||||| |||||||||| |||||||||| AAGAAAAAGC CTTCTTCAGG CAACGACAGG .......... .......... .......... 218 ATAAAAATAC TGTTCATGTT TTCCCATTTT ATCCTGGGCT TGTTTTGATA CTAATCCTTT 22735 .......... .......... .......... .......... .......... .......... 218 ACTTTTCTGT GCTATGATGC TTATATTTTG AACTTGAAAA TTGATTTTAG TTGATCATTT 22675 .......... .......... .......... .......... .......... .......... 218 TCTTCCAATT GATGATATTA TTATAAAAGC CAATAGTGGT TATATAGAAA GTTTGTAGCA 22615 .......... .......... .......... .......... .......... .......... 218 AATTACCATT TGTTGTGACA TGTTTAGTGC TAGTATACTT CTGTTATTGT TTTTACGTGT 22555 .......... .......... .......... .......... .......... .......... 218 TTACAGGGCA TTACTGCCTA ATTTCTCCTT CTGTCCTCTT CTTGTTATTG ACATTCTTAG 22495 .......... .......... .......... .......... .......... .......... 218 TCACTTATTT TTGATTGTTC GTGTATATTT AATGCACTAA GAAAGCTGTC TTGATGGACT 22435 .......... .......... .......... .......... .......... .......... 218 GCTTAGGCCC TACAGTACTC CCGTGTGATT AATCAGCGGT TTGCGCCGCT ATACTGCTAT 22375 .......... .......... .......... .......... .......... .......... 218 TACTTGACAG GTAACAGAGA CTGTGCAATG TGATAAGAAT CTGGTTGGTA GAAAGATCAG 22315 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... GTAACAGAGA CTGTGCAATG TGATAAGAAT CTGGTTGGTA GAAAGATCAG 268 AGTTTGGTGG CCATTAGATG AACTGTAAGT GCTCGTCTTC TTTTATGCTG TGGAACAATT 22255 |||||||||| |||||||||| |||| AGTTTGGTGG CCATTAGATG AACT...... .......... .......... .......... 292 TTTTTCCTTC CTCATTCTTG ACATGATAGT TTGGAATTAC TGTGTTTTTG AGTTTACTGT 22195 .......... .......... .......... .......... .......... .......... 292 ATCTGAGAAC GTTTCCAGTT CTGTATTAGT TGGGATAATT TTTTATCTGA TTTGTTGTCA 22135 .......... .......... .......... .......... .......... .......... 292 CAGATTTTAT GAAGGGACGG TTAGCAATTA TGATTCTTCC AGGAAGAAGT TCACAGTGAG 22075 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| ...ATTTTAT GAAGGGACGG TTAGCAATTA TGATTCTTCC AGGAAGAAGT TCACA..... 344 TGCAGTTTGT TTTCTGTTTC AAGAAATGGA ATTGTTCCCT TGATGTAACA CTCATATGTT 22015 .......... .......... .......... .......... .......... .......... 344 TGATTAAATT TTTTCTTATT AGGTTGACTA TACTGATGGA GAGACAGAAA AATTGAATCT 21955 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..GTTGACTA TACTGATGGA GAGACAGAAA AATTGAATCT 382 CTTGAAGGAA CGCTGGGAAC TTGTTGAAGA TGATAATATG TCAGAAGAGG TTAGTTACTG 21895 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| CTTGAAGGAA CGCTGGGAAC TTGTTGAAGA TGATAATATG TCAGAAGAG. .......... 431 ACTATATTTT GATTTATTAT GGTTATGTTT CTTTGATACA TTAGGATGCA TTTGTTGACA 21835 .......... .......... .......... .......... .......... .......... 431 GGAGCAAGTA GCCAGCGCTG ATGCAGCAGC TTCGGAAAGG TAAACCTGGC CATTAGTCAC 21775 ||||||||| |||||||||| |||||||||| ||||||||| .GAGCAAGTA GCCAGCGCTG ATGCAGCAGC TTCGGAAAG. .......... .......... 469 CATTTTCTTT ATTTGGTTTT GACTCATTTA TTTTCACCTG AAGATGCTTA CTCGATTTGA 21715 .......... .......... .......... .......... .......... .......... 469 GCCAAGTGAT CTGAATTGTC TCCCTTTGTG AAACCCTTTT GCATTTTTTC ATCTACAAGT 21655 .......... .......... .......... .......... .......... .......... 469 GTCTGTTTAG CATTATAACC ACTTGCTAGT ATGTCTCAAC TGAAGAAACA ACACAAGATT 21595 .......... .......... .......... .......... .......... .......... 469 CTTGTTGCTG TGTTGTGCTA TTTTTGTGTA CCTTTGATGT TTTCTGGGTT TGATATCTTT 21535 .......... .......... .......... .......... .......... .......... 469 GTCCAGTCTA TAGTTTCATT TTTTTTGTGA TGCATGCGAT GCTATCATAA GCAAAAAAAG 21475 .......... .......... .......... .......... .......... .......... 469 CAATTTGTCG ATTTCCTTTT TCTGTTTCCA AATATTCTGC TAACTGTAGG AGAAGTGGTA 21415 .......... .......... .......... .......... .......... .......... 469 AGTACGTTGA TATGAGTGAT AGTGATTTCC TTTATTACAT GGAAATTCTT CTTCGATGAG 21355 .......... .......... .......... .......... .......... .......... 469 CAAAGTGAAA CCAATACTCA ATTTGATGTT TGTACTCAAT TGTGTTATTA GATTATAATA 21295 .......... .......... .......... .......... .......... .......... 469 TTTTTGTAGC TGCGATTTAC TCTCTTTTCT TAATCTCTCT GTGTAGAAGT ACTTTTAACA 21235 .......... .......... .......... .......... .......... .......... 469 TTCATTCTAT TCATTGTGTT AGGTAAAGAA AGGACAGAAA TCTTCCAAGT GTAGTTAGCA 21175 .......... .......... .......... .......... .......... .......... 469 GTATGACTAT TCTTTGTTTA ACTTTGACTC TGTTTTTGAT ATGTCAGTCA TAAGAAGAAA 21115 ||| |||||||||| .......... .......... .......... .......... .......TCA TAAGAAGAAA 482 AAACCTAGGA ATGCTGAACC ATCACTAAAG CATGAAATGG ATGCTTCACC TAAAAGGTTG 21055 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| AAACCTAGGA ATGCTGAACC ATCACTAAAG CATGAAATGG ATGCTTCACC TAAAAG.... 538 GTATAATCTC CTTAACCATG TGTTTGTGTG ACATATTAAC ATCTGACGTT TTGAAAGCCT 20995 .......... .......... .......... .......... .......... .......... 538 AATGGTACCA ATGGTGGGGT TCTTTTTGCA GCAAGTC 20958 |||||| .......... .......... .......... .CAAGTC 544 hqPGS_C06HBa0046G10.1-4-_SGN-E325374+ (23214 23126,22953 22825,22364 22291,22131 22080,21992 21906,21833 21796,21127 21059,20963 20958) ******************************************************************************** EST sequence 19 +strand 444 n (File: SGN-E310295+) 1 GTCAGAGCCT TCCTGAAGAG ATTGCACTGA AGAAGGGTGA TCTCCCAATG GAGGACAACT 61 CAAATCAAGA CGGTGAGTCT AAGAAGGAAA TTGAAGCTGG AAGTGACTTG GAAGTTAAGC 121 AGGTGAGGCG CCCTTTGAGG AAGACTCCTT TAGAACCTTG TCGTAAGGAG AAAGGTGGAT 181 CCACCAGTGA TACCGAGGCT AAAAAGCTGA AGCAATCGGG TAAGAATGTT GACACCAAAA 241 ATAAGAGTCA GGTTGGTCCA TCTGCGAGAA ATAAGGAAGA TAGCAAAAAA CGTGGACATG 301 GAAAAGCTAG TCAGGAGACG TTGCCTTCAC AAGAGTCTCC TGATCGCTCT GTTAAACATG 361 ATGAAGATAA CGAAGAGGAA ATTCCCAGGA CCACTACAAA GAGAAAGCGT TCTTCAAGCA 421 AGGGCAGGGG GAGTAGGCAA GTTG Predicted gene structure (within gDNA segment 24282 to 22365): Exon 1 23682 23369 ( 314 n); cDNA 1 314 ( 314 n); score: 1.000 Intron 1 23368 23240 ( 129 n); Pd: 1.000 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 2 23239 23126 ( 114 n); cDNA 315 428 ( 114 n); score: 1.000 Intron 2 23125 22954 ( 172 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 0) Exon 3 22953 22938 ( 16 n); cDNA 429 444 ( 16 n); score: 1.000 MATCH C06HBa0046G10.1-4- SGN-E310295+ 1.000 444 1.000 C PGS_C06HBa0046G10.1-4-_SGN-E310295+ (23682 23369,23239 23126,22953 22938) Alignment (genomic DNA sequence = upper lines): GTCAGAGCCT TCCTGAAGAG ATTGCACTGA AGAAGGGTGA TCTCCCAATG GAGGACAACT 23623 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCAGAGCCT TCCTGAAGAG ATTGCACTGA AGAAGGGTGA TCTCCCAATG GAGGACAACT 60 CAAATCAAGA CGGTGAGTCT AAGAAGGAAA TTGAAGCTGG AAGTGACTTG GAAGTTAAGC 23563 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAATCAAGA CGGTGAGTCT AAGAAGGAAA TTGAAGCTGG AAGTGACTTG GAAGTTAAGC 120 AGGTGAGGCG CCCTTTGAGG AAGACTCCTT TAGAACCTTG TCGTAAGGAG AAAGGTGGAT 23503 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGTGAGGCG CCCTTTGAGG AAGACTCCTT TAGAACCTTG TCGTAAGGAG AAAGGTGGAT 180 CCACCAGTGA TACCGAGGCT AAAAAGCTGA AGCAATCGGG TAAGAATGTT GACACCAAAA 23443 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCACCAGTGA TACCGAGGCT AAAAAGCTGA AGCAATCGGG TAAGAATGTT GACACCAAAA 240 ATAAGAGTCA GGTTGGTCCA TCTGCGAGAA ATAAGGAAGA TAGCAAAAAA CGTGGACATG 23383 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAAGAGTCA GGTTGGTCCA TCTGCGAGAA ATAAGGAAGA TAGCAAAAAA CGTGGACATG 300 GAAAAGCTAG TCAGGTAAAA ACTTACTAAA TCTTTAAGTT AATGTGATGT TCAATAATTA 23323 |||||||||| |||| GAAAAGCTAG TCAG...... .......... .......... .......... .......... 314 TCTCGTCTCT GTAAATTCAT CTCCCTATTG GACTACCAAT GTGTCCCTGT ACGTCTTATC 23263 .......... .......... .......... .......... .......... .......... 314 AGTAGTTAAT TTCACTGGTG CAGGAGACGT TGCCTTCACA AGAGTCTCCT GATCGCTCTG 23203 ||||||| |||||||||| |||||||||| |||||||||| .......... .......... ...GAGACGT TGCCTTCACA AGAGTCTCCT GATCGCTCTG 351 TTAAACATGA TGAAGATAAC GAAGAGGAAA TTCCCAGGAC CACTACAAAG AGAAAGCGTT 23143 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAAACATGA TGAAGATAAC GAAGAGGAAA TTCCCAGGAC CACTACAAAG AGAAAGCGTT 411 CTTCAAGCAA GGGCAGGGTA AGCATTTTAC ATCATTGATT TCATGTATAA TGCACATTTT 23083 |||||||||| ||||||| CTTCAAGCAA GGGCAGG... .......... .......... .......... .......... 428 ATTCGAGGTA GTATTTTGAT GTGCTATTTT TTCCTGTTTC TGTAATTCTT ATGACTGAGC 23023 .......... .......... .......... .......... .......... .......... 428 TTGTTCAATT TTACTTACTT ACCATGACCC CTCCGTGTCT GTCTCATCAG TTGTTATTTC 22963 .......... .......... .......... .......... .......... .......... 428 GTTGTGCAGG GGAGTAGGCA AGTTG 22938 | |||||||||| ||||| .........G GGAGTAGGCA AGTTG 444 hqPGS_C06HBa0046G10.1-4-_SGN-E310295+ (23682 23369,23239 23126,22953 22938) ******************************************************************************** EST sequence 24 +strand 393 n (File: SGN-E301468+) 1 AAGAGATTGC ACTGAAGAAT GGTGATCTCC CAATGGAGGA CAACTCAAAT CAAGACGGTG 61 AGTCTAAGAA GGAAATTGAA GCTGGAAGTG ACTTGGAAGT TAAGCAGGTG AGGCGCCCTT 121 TGAGGAAGAC TCCTTTAGAA CCTTGTCGTA AGGAGAAAGG TGGATCCACC AGTGATACCG 181 AGGCTAAAAA GCTGAAGCAA TCGGGTAAGA ATGTTGACAC CAAAAATAAG AGTCAGGTTG 241 GTCCATCTGC GAGAAATAAG GAAGATAGCA AAAAACGTGG ACATGGAAAA GCTAGTCAGG 301 AGACGTTGCC TTCACAAGAG TCTCCTGATC GCTCTGTTAA ACATGATGAA GATAACGAAG 361 AGGAAATTCC CAGGACCACT ACAAAGAGAA AGC Predicted gene structure (within gDNA segment 24267 to 22536): Exon 1 23667 23369 ( 299 n); cDNA 1 299 ( 299 n); score: 0.997 Intron 1 23368 23240 ( 129 n); Pd: 1.000 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 2 23239 23146 ( 94 n); cDNA 300 393 ( 94 n); score: 1.000 MATCH C06HBa0046G10.1-4- SGN-E301468+ 0.997 393 1.000 C PGS_C06HBa0046G10.1-4-_SGN-E301468+ (23667 23369,23239 23146) Alignment (genomic DNA sequence = upper lines): AAGAGATTGC ACTGAAGAAG GGTGATCTCC CAATGGAGGA CAACTCAAAT CAAGACGGTG 23608 |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGAGATTGC ACTGAAGAAT GGTGATCTCC CAATGGAGGA CAACTCAAAT CAAGACGGTG 60 AGTCTAAGAA GGAAATTGAA GCTGGAAGTG ACTTGGAAGT TAAGCAGGTG AGGCGCCCTT 23548 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTCTAAGAA GGAAATTGAA GCTGGAAGTG ACTTGGAAGT TAAGCAGGTG AGGCGCCCTT 120 TGAGGAAGAC TCCTTTAGAA CCTTGTCGTA AGGAGAAAGG TGGATCCACC AGTGATACCG 23488 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAGGAAGAC TCCTTTAGAA CCTTGTCGTA AGGAGAAAGG TGGATCCACC AGTGATACCG 180 AGGCTAAAAA GCTGAAGCAA TCGGGTAAGA ATGTTGACAC CAAAAATAAG AGTCAGGTTG 23428 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGCTAAAAA GCTGAAGCAA TCGGGTAAGA ATGTTGACAC CAAAAATAAG AGTCAGGTTG 240 GTCCATCTGC GAGAAATAAG GAAGATAGCA AAAAACGTGG ACATGGAAAA GCTAGTCAGG 23368 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GTCCATCTGC GAGAAATAAG GAAGATAGCA AAAAACGTGG ACATGGAAAA GCTAGTCAG. 299 TAAAAACTTA CTAAATCTTT AAGTTAATGT GATGTTCAAT AATTATCTCG TCTCTGTAAA 23308 .......... .......... .......... .......... .......... .......... 299 TTCATCTCCC TATTGGACTA CCAATGTGTC CCTGTACGTC TTATCAGTAG TTAATTTCAC 23248 .......... .......... .......... .......... .......... .......... 299 TGGTGCAGGA GACGTTGCCT TCACAAGAGT CTCCTGATCG CTCTGTTAAA CATGATGAAG 23188 || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ........GA GACGTTGCCT TCACAAGAGT CTCCTGATCG CTCTGTTAAA CATGATGAAG 351 ATAACGAAGA GGAAATTCCC AGGACCACTA CAAAGAGAAA GC 23146 |||||||||| |||||||||| |||||||||| |||||||||| || ATAACGAAGA GGAAATTCCC AGGACCACTA CAAAGAGAAA GC 393 hqPGS_C06HBa0046G10.1-4-_SGN-E301468+ (23667 23369,23239 23146) ******************************************************************************** EST sequence 28 +strand 642 n (File: SGN-E333195+) 1 GGAGGGATGA GGCATGTTTG GAAGATATTG AGCCTGCTGT GAATGGATCT CCCAAATCAA 61 TCACAAGCAA TGGTGGTAGT TTGGAGAATG TGAGATTGAC TTCTGAAACA GAATCGCTGA 121 TGAAAGCTGG AGCTCATGAT GAGGTGGATC TCCATGATGC TTCAAAGATA CCATCTGAAT 181 CTGATGATTC AAGGGTTGAA AAGTCTACAA AGTCGGAGCC CAAGTCTATG AAGTCAGAGC 241 CCAAGTCGGA GCAACCTTCT AAGAAGAGAG GAAGGAAGAC AGTCTCTTCA ATAAACTCAG 301 CCGAATCTTC TCACCAAGCT CCTGAAGGAA GTGGAAAAGA AATTGAGAAG CTTCAAGATC 361 ATCAGAATGA TCAAAACAAA GATGACCATA GTTCAGCTTC TGAAGATCCA GTGGTTGAGC 421 AGTCCAATCT GTTGGAGAAG GAACCAGAAA CTAATCAGCA CTCTGCACCC AAAGAATCTG 481 AGGAGGAAGT TGTGGATGTT GCTCCTCCAT CCCAAGGTCA GAGCCTTCCT GAAGAGATTG 541 CACTGAAGAA GGGTGATCTC CCAATGGAGG ACAACTCAAA TCAAGACGGT GAGTCTAAGA 601 AAGAAATTGA GCTGGAAGTG ACTTGGAAGT AAGCAGGTGA GG Predicted gene structure (within gDNA segment 24798 to 22838): Exon 1 24198 23555 ( 644 n); cDNA 1 642 ( 642 n); score: 0.995 MATCH C06HBa0046G10.1-4- SGN-E333195+ 0.995 644 1.003 C PGS_C06HBa0046G10.1-4-_SGN-E333195+ (24198 23555) Alignment (genomic DNA sequence = upper lines): GGAGGGATGA GGCATGTTTG GAAGATATTG AGCCTGCTGT GAATGGATCT CCCAAATCAA 24139 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAGGGATGA GGCATGTTTG GAAGATATTG AGCCTGCTGT GAATGGATCT CCCAAATCAA 60 TCACAAGCAA TGGTGGTAGT TTGGAGAATG TGAGATTGAC TTCTGAAACA GAATCGCTGA 24079 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCACAAGCAA TGGTGGTAGT TTGGAGAATG TGAGATTGAC TTCTGAAACA GAATCGCTGA 120 TGAAAGCTGG AGCTCATGAT GAGGTGGATC TCCATGATGC TTCAAAGATA CCATCTGAAT 24019 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAAAGCTGG AGCTCATGAT GAGGTGGATC TCCATGATGC TTCAAAGATA CCATCTGAAT 180 CTGATGATTC AAGGGTTGAA AAGTCTACAA AGTCGGAGCC CAAGTCTATG AAGTCAGAGC 23959 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGATGATTC AAGGGTTGAA AAGTCTACAA AGTCGGAGCC CAAGTCTATG AAGTCAGAGC 240 CCAAGTCGGA GCAACCTTCT AAGAAGAGAG GAAGGAAGAC AGTCTCTTCA ATAAACTCAG 23899 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCAAGTCGGA GCAACCTTCT AAGAAGAGAG GAAGGAAGAC AGTCTCTTCA ATAAACTCAG 300 CCGAATCTTC TCACCAAGCT CCTGAAGGAA GTGGAAAAGA AATTGAGAAG CTTCAAGATC 23839 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCGAATCTTC TCACCAAGCT CCTGAAGGAA GTGGAAAAGA AATTGAGAAG CTTCAAGATC 360 ATCAGAATGA TCAAAACAAA GATGACCATA GTTCAGCTTC TGAAGATCCA GTGGTTGAGC 23779 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCAGAATGA TCAAAACAAA GATGACCATA GTTCAGCTTC TGAAGATCCA GTGGTTGAGC 420 AGTCCAATCT GTTGGAGAAG GAACCAGAAA CTAATCAGCA CTCTGCACCC AAAGAATCTG 23719 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTCCAATCT GTTGGAGAAG GAACCAGAAA CTAATCAGCA CTCTGCACCC AAAGAATCTG 480 AGGAGGAAGT TGTGGATGTT GCTCCTCCAT CCCAAGGTCA GAGCCTTCCT GAAGAGATTG 23659 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAGGAAGT TGTGGATGTT GCTCCTCCAT CCCAAGGTCA GAGCCTTCCT GAAGAGATTG 540 CACTGAAGAA GGGTGATCTC CCAATGGAGG ACAACTCAAA TCAAGACGGT GAGTCTAAGA 23599 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACTGAAGAA GGGTGATCTC CCAATGGAGG ACAACTCAAA TCAAGACGGT GAGTCTAAGA 600 AGGAAATTGA AGCTGGAAGT GACTTGGAAG TTAAGCAGGT GAGG 23555 | ||||||| |||||||||| |||||||||| ||||||||| |||| AAGAAATTG- AGCTGGAAGT GACTTGGAAG -TAAGCAGGT GAGG 642 hqPGS_C06HBa0046G10.1-4-_SGN-E333195+ (24198 23555) ******************************************************************************** EST sequence 21 +strand 610 n (File: SGN-E316916+) 1 AGGCGAAGCA GTCCAGACGG CTGAGAGCGG GAGGGATGAT GTTTGTTTGG AAGATATTGA 61 GCCTGCTGTG AATGGATCTC CCAAATCAAT CACAAGCAAT GGTGGTAGTT TGGAGAATGT 121 GAGATTGACT TCTGAAACAG AATCGCTGAT GAAAGCTGGA GCTCATGATG AGGTGGATCT 181 CCATGATGCT TCAAAGATAC CATCTGAATC TGATGATTCA AGGGTTGAAA AGTCTACAAA 241 GTCGGAGCCC AAGTCTATGA AGTCAGAGCC CAAGTCGGAG CAACCTTCTA AGAAGAGAGG 301 AAGGAAGACA GTCTCTTCAA TAAACTCAGC CGAATCTTCT CACCAAGCTC CTGAAGGAAG 361 TGGAAAAGAA ATTGAGAAGC TTCAAGATCA TCAGAATGAT CAAAACAAAG ATGACCATAG 421 TTCAGCTTCT GAAGATCCAG TGGTTGAGCA GTCCCATCTG TTGGAGAAGG AACCAGAAAC 481 TAATCAGCAC TCTGCACCCA AAGAATCTGA GGAGGAAGTT GTGGATGTTG CTCCTCCATC 541 CCAAGGTCAG AGCCCTTCTG AAGAGATTGC ACTGAAAAAC GCTGATCTCC CCATGGAGGA 601 CAACTCAAAT Predicted gene structure (within gDNA segment 25214 to 23008): Exon 1 24653 24638 ( 16 n); cDNA 1 16 ( 16 n); score: 1.000 Intron 1 24637 24212 ( 426 n); Pd: 1.000 (s: 0), Pa: 0.991 (s: 0.94) Exon 2 24211 23618 ( 594 n); cDNA 17 610 ( 594 n); score: 0.983 MATCH C06HBa0046G10.1-4- SGN-E316916+ 0.983 610 1.000 C PGS_C06HBa0046G10.1-4-_SGN-E316916+ (24653 24638,24211 23618) Alignment (genomic DNA sequence = upper lines): AGGCGAAGCA GTCCAGGCAT GTAGTTTGTA TATTTTCTAG TTGTTGTTGG ACATAAAACT 24594 |||||||||| |||||| AGGCGAAGCA GTCCAG.... .......... .......... .......... .......... 16 TGCAAAGTAA TGAAATATTA TTTGCATGTC AATGTTTGTG TTACAAATTT GTTCACCATC 24534 .......... .......... .......... .......... .......... .......... 16 TGAACTCCAC CTGTAATGGG TTCTCTTAGA GAACTTCAGT TTTTCCTCGA GTGAAATTTC 24474 .......... .......... .......... .......... .......... .......... 16 AAAATTGCAA CTATTTGTTC AAATTCAACA TCAAATACTC CTATTTCATC TTTAACCAAA 24414 .......... .......... .......... .......... .......... .......... 16 TATTGTCTGA ATGGGCCAGT TTCTCGTGTT GGTAAATCAA TGAAAAACAC CCTTAGGAAC 24354 .......... .......... .......... .......... .......... .......... 16 GAGCAATATC TTTGATATCT GTACTGGATG GTGTAGAGTT GAATTGAACA TGTTAGTGCA 24294 .......... .......... .......... .......... .......... .......... 16 ATGGTATGCT TATGGCGTGC AATCTCTGTT GTCTGACTGT CAGTTAAAGG TATTTAGTCA 24234 .......... .......... .......... .......... .......... .......... 16 GAAACTTTTC TTGTTTGTTC AGACGGCTGA GAGCGGGAGG GATGAGGCAT GTTTGGAAGA 24174 |||||||| |||||||||| ||||| | | |||||||||| .......... .......... ..ACGGCTGA GAGCGGGAGG GATGATGTTT GTTTGGAAGA 54 TATTGAGCCT GCTGTGAATG GATCTCCCAA ATCAATCACA AGCAATGGTG GTAGTTTGGA 24114 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATTGAGCCT GCTGTGAATG GATCTCCCAA ATCAATCACA AGCAATGGTG GTAGTTTGGA 114 GAATGTGAGA TTGACTTCTG AAACAGAATC GCTGATGAAA GCTGGAGCTC ATGATGAGGT 24054 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAATGTGAGA TTGACTTCTG AAACAGAATC GCTGATGAAA GCTGGAGCTC ATGATGAGGT 174 GGATCTCCAT GATGCTTCAA AGATACCATC TGAATCTGAT GATTCAAGGG TTGAAAAGTC 23994 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGATCTCCAT GATGCTTCAA AGATACCATC TGAATCTGAT GATTCAAGGG TTGAAAAGTC 234 TACAAAGTCG GAGCCCAAGT CTATGAAGTC AGAGCCCAAG TCGGAGCAAC CTTCTAAGAA 23934 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACAAAGTCG GAGCCCAAGT CTATGAAGTC AGAGCCCAAG TCGGAGCAAC CTTCTAAGAA 294 GAGAGGAAGG AAGACAGTCT CTTCAATAAA CTCAGCCGAA TCTTCTCACC AAGCTCCTGA 23874 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGAGGAAGG AAGACAGTCT CTTCAATAAA CTCAGCCGAA TCTTCTCACC AAGCTCCTGA 354 AGGAAGTGGA AAAGAAATTG AGAAGCTTCA AGATCATCAG AATGATCAAA ACAAAGATGA 23814 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAAGTGGA AAAGAAATTG AGAAGCTTCA AGATCATCAG AATGATCAAA ACAAAGATGA 414 CCATAGTTCA GCTTCTGAAG ATCCAGTGGT TGAGCAGTCC AATCTGTTGG AGAAGGAACC 23754 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| |||||||||| CCATAGTTCA GCTTCTGAAG ATCCAGTGGT TGAGCAGTCC CATCTGTTGG AGAAGGAACC 474 AGAAACTAAT CAGCACTCTG CACCCAAAGA ATCTGAGGAG GAAGTTGTGG ATGTTGCTCC 23694 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAACTAAT CAGCACTCTG CACCCAAAGA ATCTGAGGAG GAAGTTGTGG ATGTTGCTCC 534 TCCATCCCAA GGTCAGAGCC TTCCTGAAGA GATTGCACTG AAGAAGGGTG ATCTCCCAAT 23634 |||||||||| |||||||||| | ||||||| |||||||||| || || | || ||||||| || TCCATCCCAA GGTCAGAGCC CTTCTGAAGA GATTGCACTG AAAAACGCTG ATCTCCCCAT 594 GGAGGACAAC TCAAAT 23618 |||||||||| |||||| GGAGGACAAC TCAAAT 610 hqPGS_C06HBa0046G10.1-4-_SGN-E316916+ (24211 23618) Total number of EST alignments reported: 29 ________________________________________________________________________________ Predicted gene locations (3) in segment 1 to 25558: PGL 1 (+ strand): 3133 12269 AGS-1 (3133 3477,4651 4732,6727 6805,6883 6980,7066 7122,7697 7784,9492 9598,11630 11737,11917 12269) SCR (e 0.994 d 0.998 a 0.999,e 1.000 d 0.815 a 0.328,e 1.000 d 0.900 a 0.975,e 1.000 d 0.979 a 0.985,e 1.000 d 0.985 a 0.648,e 1.000 d 0.915 a 0.976,e 0.981 d 0.997 a 0.997,e 0.981 d 0.999 a 0.912,e 0.994) Exon 1 3133 3477 ( 345 n); score: 0.994 Intron 1 3478 4650 (1173 n); Pd: 0.998 Pa: 0.999 Exon 2 4651 4732 ( 82 n); score: 1.000 Intron 2 4733 6726 (1994 n); Pd: 0.815 Pa: 0.328 Exon 3 6727 6805 ( 79 n); score: 1.000 Intron 3 6806 6882 ( 77 n); Pd: 0.900 Pa: 0.975 Exon 4 6883 6980 ( 98 n); score: 1.000 Intron 4 6981 7065 ( 85 n); Pd: 0.979 Pa: 0.985 Exon 5 7066 7122 ( 57 n); score: 1.000 Intron 5 7123 7696 ( 574 n); Pd: 0.985 Pa: 0.648 Exon 6 7697 7784 ( 88 n); score: 1.000 Intron 6 7785 9491 (1707 n); Pd: 0.915 Pa: 0.976 Exon 7 9492 9598 ( 107 n); score: 0.981 Intron 7 9599 11629 (2031 n); Pd: 0.997 Pa: 0.997 Exon 8 11630 11737 ( 108 n); score: 0.981 Intron 8 11738 11916 ( 179 n); Pd: 0.999 Pa: 0.912 Exon 9 11917 12269 ( 353 n); score: 0.994 PGS (3133 3477,4651 4732,6727 6805,6883 6980,7066 7090) SGN-E542618+ PGS (3133 3477,4651 4732,6727 6805,6883 6936) SGN-E542658+ PGS (3268 3477,4651 4732,6727 6805,6883 6980,7066 7122) SGN-E301411+ PGS (3285 3477,4651 4732,6727 6805,6883 6980,7066 7122,7697 7781) SGN-E323609+ PGS (7074 7122,7697 7784,9492 9598,11630 11737,11917 12269) SGN-E542617- PGS (9558 9598,11630 11737,11917 12269) SGN-E542657- 3-phase translation of AGS-1 (+strand): . . . . . . 3133 AAAAAATATAAAACAAAACACCTTCCATAAACAAAGTGACGACGACGTCGTATTAAGTCC K K Y K T K H L P - T K - R R R R I K S K N I K Q N T F H K Q S D D D V V L S P K I - N K T P S I N K V T T T S Y - V . . . . . . 3193 CTCCAGAAACCCCAAATCAAAATTCTCGGTCCTATAAACCCCAGGTGTTTTCTTCTTCTT L Q K P Q I K I L G P I N P R C F L L L S R N P K S K F S V L - T P G V F F F F P P E T P N Q N S R S Y K P Q V F S S S . . . . . . 3253 CTTCCCAATTCTCAAAATCTTACCATTTCTCGCCATGGATGAAGAAGAGATTGAGAAAGC L P N S Q N L T I S R H G - R R D - E S F P I L K I L P F L A M D E E E I E K A S S Q F S K S Y H F S P W M K K R L R K . . . . . . 3313 AGTCGTAGCGTATTTGAAGAAGAAGGGTTTCAAGCAAACGGAGATTGCTTTTCAAGAAGA S R S V F E E E G F Q A N G D C F S R R V V A Y L K K K G F K Q T E I A F Q E E Q S - R I - R R R V S S K R R L L F K K . . . . . . 3373 ACAGCAAGTGAATAAAAACAGTTCTTCTTCAATCCATAATAACAACAACTCAGCAAATTC T A S E - K Q F F F N P - - Q Q L S K F Q Q V N K N S S S S I H N N N N S A N S N S K - I K T V L L Q S I I T T T Q Q I . . . . . : . 3433 TCAGATTGACCCCGACTTAACCAAAAAAATCCTTTCTTTCTCTGA : ATTTGAAACCAGTCC S D - P R L N Q K N P F F L - : I - N Q S Q I D P D L T K K I L S F S E : F E T S P L R L T P T - P K K S F L S L : N L K P V . . . . . . 4666 CCAACGATACCAAGAAGAATATAGCAAACTTCGGTCATGGGCCTATAGTTCACTGGATCT P T I P R R I - Q T S V M G L - F T G S Q R Y Q E E Y S K L R S W A Y S S L D L P N D T K K N I A N F G H G P I V H W I . : . . . . . 4726 GTATAAG : CATGAGTTGCTTCGTGTTTTATATCCTGTATTTATTCATTGTTTCATGGAACT V - : A - V A S C F I S C I Y S L F H G T Y K : H E L L R V L Y P V F I H C F M E L C I S : M S C F V F Y I L Y L F I V S W N . . . : . . . 6780 TGTCGCTAGAGGTTATATTCAAGAAG : CACGAGCCTTTTTCAATAGTTACCGTGAAGACCA C R - R L Y S R S : T S L F Q - L P - R P V A R G Y I Q E : A R A F F N S Y R E D H L S L E V I F K K : H E P F S I V T V K T . . . . . . 6917 TGAGATGACGCATCTGCGTGATCTTCAGAAGTTAGAAGGCGTCCTCTCACCTTCCCATCT - D D A S A - S S E V R R R P L T F P S E M T H L R D L Q K L E G V L S P S H L M R - R I C V I F R S - K A S S H L P I . : . . . . . 6977 AGAG : GAAATGGAATTTGCTCATTCTATACGGGTGAGCAAAGTGAACATTAAGATGTGTCA R : G N G I C S F Y T G E Q S E H - D V S E : E M E F A H S I R V S K V N I K M C Q - R : K W N L L I L Y G - A K - T L R C V . : . . . . . 7122 G : TACTCCTATGACCTTCTGTTGCAATACTTGCACAAGACAGAATCTATTACAATGCTTGG : V L L - P S V A I L A Q D R I Y Y N A W : Y S Y D L L L Q Y L H K T E S I T M L G S : T P M T F C C N T C T R Q N L L Q C L . . . : . . . 7756 GATCATCAATGAGCGCATTAATTTTCAAG : TTTCTCCTGGACAGCCTGGCTCTATATCTGG D H Q - A H - F S S : F S W T A W L Y I W I I N E R I N F Q : V S P G Q P G S I S G G S S M S A L I F K : F L L D S L A L Y L . . . . . . 9523 TGATACGGAAGTTGTAACTCTTGTTGCAAGTGGCCATGATGCTAGTCTCATAAATCAGAA - Y G S C N S C C K W P - C - S H K S E D T E V V T L V A S G H D A S L I N Q K V I R K L - L L L Q V A M M L V S - I R . . : . . . . 9583 AGAAGTTCTTTGGGGG : ATGCTTGAAGATTCTTTAGAAGAACGGTTAGAAAAGACTGGAGG R S S L G : D A - R F F R R T V R K D W R E V L W G : M L E D S L E E R L E K T G G K K F F G G : C L K I L - K N G - K R L E . . . . . . 11674 TTCTGTTCCAGATTCGGAAAAGTTTGATGGAGATCCGAAGGAAGGAGAAGTGGAAGAAAA F C S R F G K V - W R S E G R R S G R K S V P D S E K F D G D P K E G E V E E N V L F Q I R K S L M E I R R K E K W K K . : . . . . . 11734 CAAG : AAGAAGTCAATTGAAGGTGGTAAACATGGTGCACCTCTTAAAAAACTAAAGAAGGA Q : E E V N - R W - T W C T S - K T K E G K : K K S I E G G K H G A P L K K L K K D T R : R S Q L K V V N M V H L L K N - R R . . . . . . 11973 CAAAGTTGGGATTGCAACAGGGAAATCTTCTCGTACAGAGAGCAGCACAGTGACAACTGC Q S W D C N R E I F S Y R E Q H S D N C K V G I A T G K S S R T E S S T V T T A T K L G L Q Q G N L L V Q R A A Q - Q L . . . . . . 12033 ACCACGTGTCAAACCGGAACTTGCTTTACCTGCTATGTACTCCTCAAACCTATTATCTTA T T C Q T G T C F T C Y V L L K P I I L P R V K P E L A L P A M Y S S N L L S Y H H V S N R N L L Y L L C T P Q T Y Y L . . . . . . 12093 CTTACCAAAAGAAAAAGAGAGGGCCCTATTGTGTTTTTCTCTCCCACGACATCATCCCTG L T K R K R E G P I V F F S P T T S S L L P K E K E R A L L C F S L P R H H P C T Y Q K K K R G P Y C V F L S H D I I P . . . . . . 12153 TACTTGGAAAAGTCATTCTTGTTGGATGTCATAATTTTATTTGAAATACAGTCATCATGA Y L E K S F L L D V I I L F E I Q S S - T W K S H S C W M S - F Y L K Y S H H D V L G K V I L V G C H N F I - N T V I M . . . . . . 12213 TCCATTAGAAAACATCAAGTGAAACGGCTATACAAAGAAGTTGCATAAAAAAGGAAA S I R K H Q V K R L Y K E V A - K R K P L E N I K - N G Y T K K L H K K G I H - K T S S E T A I Q R S C I K K E Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-4+_PGL-1_AGS-1_PPS_1 (3230 3477,4651 4732,6727 6805,6883 6980,7066 7122,7697 7784,9492 9598,11630 11737,11917 12186) (frame '2'; 1134 bp, 378 residues) 1 TPGVFFFFFP ILKILPFLAM DEEEIEKAVV AYLKKKGFKQ TEIAFQEEQQ VNKNSSSSIH 61 NNNNSANSQI DPDLTKKILS FSEFETSPQR YQEEYSKLRS WAYSSLDLYK HELLRVLYPV 121 FIHCFMELVA RGYIQEARAF FNSYREDHEM THLRDLQKLE GVLSPSHLEE MEFAHSIRVS 181 KVNIKMCQYS YDLLLQYLHK TESITMLGII NERINFQVSP GQPGSISGDT EVVTLVASGH 241 DASLINQKEV LWGMLEDSLE ERLEKTGGSV PDSEKFDGDP KEGEVEENKK KSIEGGKHGA 301 PLKKLKKDKV GIATGKSSRT ESSTVTTAPR VKPELALPAM YSSNLLSYLP KEKERALLCF 361 SLPRHHPCTW KSHSCWMS- AGS-2 (10378 10401,10483 10724) SCR (e 0.750 d 0.427 a 0.000,e 0.901) Exon 1 10378 10401 ( 24 n); score: 0.750 Intron 1 10402 10482 ( 81 n); Pd: 0.427 Pa: 0.000 Exon 2 10483 10724 ( 242 n); score: 0.901 PGS (10378 10401,10483 10724) SGN-E322543- PGS (10465 10724) SGN-E380042+ PGS (10465 10721) SGN-E209785+ PGS (10477 10670) SGN-E348402+ PGS (10479 10724) SGN-E332440+ PGS (10480 10724) SGN-E220828- PGS (10480 10724) SGN-E220902- 3-phase translation of AGS-2 (+strand): . . . : . . . 10378 ATAAAACGAAGAGAATGTGGAGCT : TTGGGGTGGCCCAGTGGTTTGAGTTTGTGACTTCCA I K R R E C G A : L G W P S G L S L - L P - N E E N V E L : W G G P V V - V C D F H K T K R M W S : F G V A Q W F E F V T S . . . . . . 10519 TGTTGGAGGTCTCAAGTTCGAAACCCCTTGCCAGCGAAAGCAAGGAGTTTGCCTTCTGGG C W R S Q V R N P L P A K A R S L P S G V G G L K F E T P C Q R K Q G V C L L G M L E V S S S K P L A S E S K E F A F W . . . . . . 10579 TCGAGCTCGTCGCACCAGGTTTGTCTAGTGCGTGTTACCTCTCATATGTGGTTTGCGAGC S S S S H Q V C L V R V T S H M W F A S R A R R T R F V - C V L P L I C G L R A V E L V A P G L S S A C Y L S Y V V C E . . . . . . 10639 TATTGCATAGGAGCGGGGTTTTTACCCTGTGCGCACCCAAAGGGTAGCGGCTGTGGGTTT Y C I G A G F L P C A H P K G S G C G F I A - E R G F Y P V R T Q R V A A V G F L L H R S G V F T L C A P K G - R L W V . . . 10699 CCCTTGTCATAAAAAAAGAAGAAGAA P L S - K K K K P C H K K R R R S L V I K K E E E Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-4+_PGL-1_AGS-2_PPS_1 (10380 10401,10483 10685) (frame '0'; 222 bp, 74 residues) 1 KTKRMWSFGV AQWFEFVTSM LEVSSSKPLA SESKEFAFWV ELVAPGLSSA CYLSYVVCEL 61 LHRSGVFTLC APKG- >C06HBa0046G10.1-4+_PGL-1_AGS-2_PPS_2 (10513 10710) (frame '1'; 195 bp, 65 residues) 1 LPCWRSQVRN PLPAKARSLP SGSSSSHQVC LVRVTSHMWF ASYCIGAGFL PCAHPKGSGC 61 GFPLS- PGL 2 (+ strand): 12783 18632 AGS-1 (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17950,18063 18158,18252 18327,18408 18632) SCR (e 1.000 d 0.980 a 0.527,e 1.000 d 0.906 a 0.980,e 1.000 d 0.995 a 0.000,e 1.000 d 0.992 a 0.952,e 1.000 d 0.978 a 0.764,e 1.000 d 0.880 a 0.958,e 1.000 d 0.000 a 0.990,e 1.000 d 0.960 a 0.000,e 1.000 d 0.975 a 0.489,e 0.996) Exon 1 12783 12841 ( 59 n); score: 1.000 Intron 1 12842 12933 ( 92 n); Pd: 0.980 Pa: 0.527 Exon 2 12934 13003 ( 70 n); score: 1.000 Intron 2 13004 13140 ( 137 n); Pd: 0.906 Pa: 0.980 Exon 3 13141 13180 ( 40 n); score: 1.000 Intron 3 13181 15923 (2743 n); Pd: 0.995 Pa: 0.000 Exon 4 15924 16088 ( 165 n); score: 1.000 Intron 4 16089 16221 ( 133 n); Pd: 0.992 Pa: 0.952 Exon 5 16222 16298 ( 77 n); score: 1.000 Intron 5 16299 16516 ( 218 n); Pd: 0.978 Pa: 0.764 Exon 6 16517 16633 ( 117 n); score: 1.000 Intron 6 16634 17684 (1051 n); Pd: 0.880 Pa: 0.958 Exon 7 17685 17950 ( 266 n); score: 1.000 Intron 7 17951 18062 ( 112 n); Pd: 0.000 Pa: 0.990 Exon 8 18063 18158 ( 96 n); score: 1.000 Intron 8 18159 18251 ( 93 n); Pd: 0.960 Pa: 0.000 Exon 9 18252 18327 ( 76 n); score: 1.000 Intron 9 18328 18407 ( 80 n); Pd: 0.975 Pa: 0.489 Exon 10 18408 18632 ( 225 n); score: 0.996 PGS (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17950,18063 18092) SGN-E262068+ PGS (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17728) SGN-E252040+ PGS (12978 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17755) SGN-E251386+ PGS (16572 16633,17685 17950,18063 18158,18252 18327) SGN-E203439+ PGS (16595 16633,17685 17950,18063 18158,18252 18325) SGN-E241154+ PGS (17704 17950,18063 18158,18252 18327,18408 18551) SGN-E253821+ PGS (17794 17950,18063 18158,18252 18327,18408 18632) SGN-E244420+ PGS (17885 17950,18063 18158,18252 18327,18408 18548) SGN-E256654+ PGS (17885 17950,18063 18158,18252 18327,18408 18546) SGN-E255083+ 3-phase translation of AGS-1 (+strand): . . . . . . : 12783 TTGAGTAATGCATCATTGCCCTCTGTTAGCTTCTATACATTTGTTAATACACACAATGG : A L S N A S L P S V S F Y T F V N T H N G : - V M H H C P L L A S I H L L I H T M : D E - C I I A L C - L L Y I C - Y T Q W : . . . . . . 12935 CTAAACTGTGCATCAATATCACATGATGGATCACTAGTGGCTGGTGGATTCTCAGATTCA L N C A S I S H D G S L V A G G F S D S - T V H Q Y H M M D H - W L V D S Q I H T K L C I N I T - W I T S G W W I L R F . : . . . . : . 12995 TCTGTGAAG : GTCTGGGACATGGCAAAGCTTGGGGAACAAACTGGTCCAT : CTTTTTTGCAG S V K : V W D M A K L G E Q T G P : S F L Q L - R : S G T W Q S L G N K L V H : L F C R I C E : G L G H G K A W G T N W S I : F F A . . . . . . 15935 GGGGAGACTGATTCACCTTCAAGTGAGCATGTGCTTGGCGCAAATGGTGGAAGAAGATGT G E T D S P S S E H V L G A N G G R R C G R L I H L Q V S M C L A Q M V E E D V G G D - F T F K - A C A W R K W W K K M . . . . . . 15995 TATACATTACTTCAGGGCCATTCAGGGCCTGTTTATTCTGCATCATTCAGTCCGCACGGG Y T L L Q G H S G P V Y S A S F S P H G I H Y F R A I Q G L F I L H H S V R T G L Y I T S G P F R A C L F C I I Q S A R . . . . : . . 16055 GATTTTCTTCTTTCTTCATCATCAGATTCAACTG : TTCGCTTATGGAGCACAAAGTTAAAT D F L L S S S S D S T : V R L W S T K L N I F F F L H H Q I Q L : F A Y G A Q S - M G F S S F F I I R F N C : S L M E H K V K . . . . . . : 16248 GCTAATGTTGTTTGCTACAAAGGCCACAATTACCCAGTATGGGATGTTCAG : TTTAGCCCA A N V V C Y K G H N Y P V W D V Q : F S P L M L F A T K A T I T Q Y G M F S : L A Q C - C C L L Q R P Q L P S M G C S : V - P . . . . . . 16526 GCTGGACATTATTTTGCCAGTTCTTCACATGACCGCACTGCAAGGATCTGGTCTGTGGAC A G H Y F A S S S H D R T A R I W S V D L D I I L P V L H M T A L Q G S G L W T S W T L F C Q F F T - P H C K D L V C G . . . . . : . 16586 AGGATACAGCCTCTAAGAATAATGGCCGGACACTTGTCAGATGTAGAT : TGTGTGCGATGG R I Q P L R I M A G H L S D V D : C V R W G Y S L - E - W P D T C Q M - I : V C D G Q D T A S K N N G R T L V R C R : L C A M . . . . . . 17697 CACGCAAATTGCAATTACATTGCAACCGGATCAAGCGACAAAACTGTACGCTTGTGGGAC H A N C N Y I A T G S S D K T V R L W D T Q I A I T L Q P D Q A T K L Y A C G T A R K L Q L H C N R I K R Q N C T L V G . . . . . . 17757 GTCCAGAGTGGTGACTGTGTCAGAATTTTCATTGGTCATAGGAGTATGATCCTATCATTG V Q S G D C V R I F I G H R S M I L S L S R V V T V S E F S L V I G V - S Y H W R P E W - L C Q N F H W S - E Y D P I I . . . . . . 17817 GCAATGTCTCCTGATGGTCGATATATGGCATCCGGTGATGAAGATGGAACAGTCATGATG A M S P D G R Y M A S G D E D G T V M M Q C L L M V D I W H P V M K M E Q S - C G N V S - W S I Y G I R - - R W N S H D . . . . . . 17877 TGGGATCTCTCAAGTGGACGTTGTATTACTCCTTTGACCGGTCATTCCTCTTGTGTGTGG W D L S S G R C I T P L T G H S S C V W G I S Q V D V V L L L - P V I P L V C G V G S L K W T L Y Y S F D R S F L L C V . . : . . . . 17937 TCACTTGCTTTTAG : TGGTGAAGGTTCACTTCTTGCATCGGGCTCTGCGGATTGCACTGTA S L A F S : G E G S L L A S G S A D C T V H L L L : V V K V H F L H R A L R I A L - V T C F - : W - R F T S C I G L C G L H C . . . . . : . 18109 AAATTATGGGATGTAACCACAAGCACCAGAGCGCCACTAAAAGAAGAGAA : CAAATCTGGA K L W D V T T S T R A P L K E E N : K S G N Y G M - P Q A P E R H - K K R : T N L E K I M G C N H K H Q S A T K R R E : Q I W . . . . . . 18262 GGTAACAATAGGCTTCGGTTATTGAAGACCCTACCTACCAAAGCTACACCAATCTATGCT G N N R L R L L K T L P T K A T P I Y A V T I G F G Y - R P Y L P K L H Q S M L R - Q - A S V I E D P T Y Q S Y T N L C . : . . . . . 18322 TTGCAC : TTTTCACGAAGAAATCTTTTATTTGCTGCTGGAGCTTTCTCGAAAAGTGCACGA L H : F S R R N L L F A A G A F S K S A R C T : F H E E I F Y L L L E L S R K V H E F A : L F T K K S F I C C W S F L E K C T . . . . . . 18462 GTCCCTTGCTTTAAAGTCTCTTGACGTGAGATTACATCATGGTTCCCTTGCAAATTTTGG V P C F K V S - R E I T S W F P C K F W S L A L K S L D V R L H H G S L A N F G S P L L - S L L T - D Y I M V P L Q I L . . . . . . 18522 TGCAATCATCAATGGCAAGTTTTACTTCTCAGACCTCGGTTTTGTTAGAGGAGCAACTCA C N H Q W Q V L L L R P R F C - R S N S A I I N G K F Y F S D L G F V R G A T H V Q S S M A S F T S Q T S V L L E E Q L . . . . . . 18582 TGTTGGTGACACGTGCATCTCACAATACAATTTTGCATCACATGGTGTAAT C W - H V H L T I Q F C I T W C N V G D T C I S Q Y N F A S H G V M L V T R A S H N T I L H H M V - Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-4+_PGL-2_AGS-1_PPS_1 (12783 12841,12934 13003,13141 13180,15924 16088,16222 16298,16517 16633,17685 17950,18063 18158,18252 18327,18408 18485) (frame '1'; 1041 bp, 347 residues) 1 LSNASLPSVS FYTFVNTHNG LNCASISHDG SLVAGGFSDS SVKVWDMAKL GEQTGPSFLQ 61 GETDSPSSEH VLGANGGRRC YTLLQGHSGP VYSASFSPHG DFLLSSSSDS TVRLWSTKLN 121 ANVVCYKGHN YPVWDVQFSP AGHYFASSSH DRTARIWSVD RIQPLRIMAG HLSDVDCVRW 181 HANCNYIATG SSDKTVRLWD VQSGDCVRIF IGHRSMILSL AMSPDGRYMA SGDEDGTVMM 241 WDLSSGRCIT PLTGHSSCVW SLAFSGEGSL LASGSADCTV KLWDVTTSTR APLKEENKSG 301 GNNRLRLLKT LPTKATPIYA LHFSRRNLLF AAGAFSKSAR VPCFKVS- PGL 3 (- strand): 24211 20093 AGS-1 (20268 20093) SCR (e 0.977) Exon 1 20268 20093 ( 176 n); score: 0.977 PGS (20268 20093) SGN-E226670- 3-phase translation of AGS-1 (-strand): . . . . . . 20268 GTTTTTTTTTTCAGTTTAAAGCATATTGTTAGAGATGTAATTGGTTAGATTTATCATTTT V F F F S L K H I V R D V I G - I Y H F F F F S V - S I L L E M - L V R F I I L F F F Q F K A Y C - R C N W L D L S F . . . . . . 20208 GGTTGCTAGCTGTATTCACTTTTGTTACTTTTAGCCCTCTTAGCGTAGGAAGGAGATACT G C - L Y S L L L L L A L L A - E G D T V A S C I H F C Y F - P S - R R K E I L W L L A V F T F V T F S P L S V G R R Y . . . . . . 20148 CTGTTGATGAGTATTTTATGGATGCGTTGGATCATAAATTACCATCTCTTCTCTTG L L M S I L W M R W I I N Y H L F S C - - V F Y G C V G S - I T I S S L S V D E Y F M D A L D H K L P S L L L Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (+strand): . . . . . . 20093 CAAGAGAAGAGATGGTAATTTATGATCCAACGCATCCATAAAATACTCATCAACAGAGTA Q E K R W - F M I Q R I H K I L I N R V K R R D G N L - S N A S I K Y S S T E Y R E E M V I Y D P T H P - N T H Q Q S . . . . . . 20153 TCTCCTTCCTACGCTAAGAGGGCTAAAAGTAACAAAAGTGAATACAGCTAGCAACCAAAA S P S Y A K R A K S N K S E Y S - Q P K L L P T L R G L K V T K V N T A S N Q N I S F L R - E G - K - Q K - I Q L A T K . . . . . . 20213 TGATAAATCTAACCAATTACATCTCTAACAATATGCTTTAAACTGAAAAAAAAAAC - - I - P I T S L T I C F K L K K K D K S N Q L H L - Q Y A L N - K K K M I N L T N Y I S N N M L - T E K K N Maximal non-overlapping open reading frames (>= 64 codons): none AGS-2 (24211 23369,23239 23126,22953 22825,22364 22291,22131 22080,21992 21906,21833 21796,21127 21059,20963 20541) SCR (e 0.995 d 1.000 a 0.960,e 1.000 d 0.998 a 0.991,e 1.000 d 1.000 a 0.678,e 1.000 d 0.984 a 0.997,e 1.000 d 0.583 a 1.000,e 1.000 d 1.000 a 0.996,e 1.000 d 0.964 a 0.999,e 1.000 d 0.996 a 0.972,e 1.000) Exon 1 24211 23369 ( 843 n); score: 0.995 Intron 1 23368 23240 ( 129 n); Pd: 1.000 Pa: 0.960 Exon 2 23239 23126 ( 114 n); score: 1.000 Intron 2 23125 22954 ( 172 n); Pd: 0.998 Pa: 0.991 Exon 3 22953 22825 ( 129 n); score: 1.000 Intron 3 22824 22365 ( 460 n); Pd: 1.000 Pa: 0.678 Exon 4 22364 22291 ( 74 n); score: 1.000 Intron 4 22290 22132 ( 159 n); Pd: 0.984 Pa: 0.997 Exon 5 22131 22080 ( 52 n); score: 1.000 Intron 5 22079 21993 ( 87 n); Pd: 0.583 Pa: 1.000 Exon 6 21992 21906 ( 87 n); score: 1.000 Intron 6 21905 21834 ( 72 n); Pd: 1.000 Pa: 0.996 Exon 7 21833 21796 ( 38 n); score: 1.000 Intron 7 21795 21128 ( 668 n); Pd: 0.964 Pa: 0.999 Exon 8 21127 21059 ( 69 n); score: 1.000 Intron 8 21058 20964 ( 95 n); Pd: 0.996 Pa: 0.972 Exon 9 20963 20541 ( 423 n); score: 1.000 PGS (21833 21796,21127 21059,20963 20541) SGN-E286841+ PGS (23214 23126,22953 22825,22364 22291,22131 22080,21992 21906,21833 21796,21127 21059,20963 20958) SGN-E325374+ PGS (23682 23369,23239 23126,22953 22938) SGN-E310295+ PGS (23667 23369,23239 23146) SGN-E301468+ PGS (24198 23555) SGN-E333195+ PGS (24211 23618) SGN-E316916+ 3-phase translation of AGS-2 (-strand): . . . . . . 24211 ACGGCTGAGAGCGGGAGGGATGAGGCATGTTTGGAAGATATTGAGCCTGCTGTGAATGGA T A E S G R D E A C L E D I E P A V N G R L R A G G M R H V W K I L S L L - M D G - E R E G - G M F G R Y - A C C E W . . . . . . 24151 TCTCCCAAATCAATCACAAGCAATGGTGGTAGTTTGGAGAATGTGAGATTGACTTCTGAA S P K S I T S N G G S L E N V R L T S E L P N Q S Q A M V V V W R M - D - L L K I S Q I N H K Q W W - F G E C E I D F - . . . . . . 24091 ACAGAATCGCTGATGAAAGCTGGAGCTCATGATGAGGTGGATCTCCATGATGCTTCAAAG T E S L M K A G A H D E V D L H D A S K Q N R - - K L E L M M R W I S M M L Q R N R I A D E S W S S - - G G S P - C F K . . . . . . 24031 ATACCATCTGAATCTGATGATTCAAGGGTTGAAAAGTCTACAAAGTCGGAGCCCAAGTCT I P S E S D D S R V E K S T K S E P K S Y H L N L M I Q G L K S L Q S R S P S L D T I - I - - F K G - K V Y K V G A Q V . . . . . . 23971 ATGAAGTCAGAGCCCAAGTCGGAGCAACCTTCTAAGAAGAGAGGAAGGAAGACAGTCTCT M K S E P K S E Q P S K K R G R K T V S - S Q S P S R S N L L R R E E G R Q S L Y E V R A Q V G A T F - E E R K E D S L . . . . . . 23911 TCAATAAACTCAGCCGAATCTTCTCACCAAGCTCCTGAAGGAAGTGGAAAAGAAATTGAG S I N S A E S S H Q A P E G S G K E I E Q - T Q P N L L T K L L K E V E K K L R F N K L S R I F S P S S - R K W K R N - . . . . . . 23851 AAGCTTCAAGATCATCAGAATGATCAAAACAAAGATGACCATAGTTCAGCTTCTGAAGAT K L Q D H Q N D Q N K D D H S S A S E D S F K I I R M I K T K M T I V Q L L K I E A S R S S E - S K Q R - P - F S F - R . . . . . . 23791 CCAGTGGTTGAGCAGTCCAATCTGTTGGAGAAGGAACCAGAAACTAATCAGCACTCTGCA P V V E Q S N L L E K E P E T N Q H S A Q W L S S P I C W R R N Q K L I S T L H S S G - A V Q S V G E G T R N - S A L C . . . . . . 23731 CCCAAAGAATCTGAGGAGGAAGTTGTGGATGTTGCTCCTCCATCCCAAGGTCAGAGCCTT P K E S E E E V V D V A P P S Q G Q S L P K N L R R K L W M L L L H P K V R A F T Q R I - G G S C G C C S S I P R S E P . . . . . . 23671 CCTGAAGAGATTGCACTGAAGAAGGGTGATCTCCCAATGGAGGACAACTCAAATCAAGAC P E E I A L K K G D L P M E D N S N Q D L K R L H - R R V I S Q W R T T Q I K T S - R D C T E E G - S P N G G Q L K S R . . . . . . 23611 GGTGAGTCTAAGAAGGAAATTGAAGCTGGAAGTGACTTGGAAGTTAAGCAGGTGAGGCGC G E S K K E I E A G S D L E V K Q V R R V S L R R K L K L E V T W K L S R - G A R - V - E G N - S W K - L G S - A G E A . . . . . . 23551 CCTTTGAGGAAGACTCCTTTAGAACCTTGTCGTAAGGAGAAAGGTGGATCCACCAGTGAT P L R K T P L E P C R K E K G G S T S D L - G R L L - N L V V R R K V D P P V I P F E E D S F R T L S - G E R W I H Q - . . . . . . 23491 ACCGAGGCTAAAAAGCTGAAGCAATCGGGTAAGAATGTTGACACCAAAAATAAGAGTCAG T E A K K L K Q S G K N V D T K N K S Q P R L K S - S N R V R M L T P K I R V R Y R G - K A E A I G - E C - H Q K - E S . . . . . . 23431 GTTGGTCCATCTGCGAGAAATAAGGAAGATAGCAAAAAACGTGGACATGGAAAAGCTAGT V G P S A R N K E D S K K R G H G K A S L V H L R E I R K I A K N V D M E K L V G W S I C E K - G R - Q K T W T W K S - . : . . . . . 23371 CAG : GAGACGTTGCCTTCACAAGAGTCTCCTGATCGCTCTGTTAAACATGATGAAGATAAC Q : E T L P S Q E S P D R S V K H D E D N R : R R C L H K S L L I A L L N M M K I T S : G D V A F T R V S - S L C - T - - R - . . . . . . : 23182 GAAGAGGAAATTCCCAGGACCACTACAAAGAGAAAGCGTTCTTCAAGCAAGGGCAGG : GGG E E E I P R T T T K R K R S S S K G R : G K R K F P G P L Q R E S V L Q A R A G : G R R G N S Q D H Y K E K A F F K Q G Q : G . . . . . . 22950 AGTAGGCAAGTTGTGCAGAAGAGTGTGCCTACGCCCGAGTCTCCCGACAATTCAACTAAA S R Q V V Q K S V P T P E S P D N S T K V G K L C R R V C L R P S L P T I Q L N E - A S C A E E C A Y A R V S R Q F N - . . . . . . 22890 CATATAAGCGACGAAGATGAGACTGACACAAGTGCAAAGAAAAAGCCTTCTTCAGGCAAC H I S D E D E T D T S A K K K P S S G N I - A T K M R L T Q V Q R K S L L Q A T T Y K R R R - D - H K C K E K A F F R Q . : . . . . . 22830 GACAGG : GTAACAGAGACTGTGCAATGTGATAAGAATCTGGTTGGTAGAAAGATCAGAGTT D R : V T E T V Q C D K N L V G R K I R V T G : - Q R L C N V I R I W L V E R S E F R Q : G N R D C A M - - E S G W - K D Q S . . : . . . . 22310 TGGTGGCCATTAGATGAACT : ATTTTATGAAGGGACGGTTAGCAATTATGATTCTTCCAGG W W P L D E L : F Y E G T V S N Y D S S R G G H - M N : Y F M K G R L A I M I L P G L V A I R - T : I L - R D G - Q L - F F Q . . : . . . . 22091 AAGAAGTTCACA : GTTGACTATACTGATGGAGAGACAGAAAAATTGAATCTCTTGAAGGAA K K F T : V D Y T D G E T E K L N L L K E R S S Q : L T I L M E R Q K N - I S - R N E E V H : S - L Y - W R D R K I E S L E G . . . . : . . 21944 CGCTGGGAACTTGTTGAAGATGATAATATGTCAGAAGAG : GAGCAAGTAGCCAGCGCTGAT R W E L V E D D N M S E E : E Q V A S A D A G N L L K M I I C Q K R : S K - P A L M T L G T C - R - - Y V R R : G A S S Q R - . . : . . . . 21812 GCAGCAGCTTCGGAAAG : TCATAAGAAGAAAAAACCTAGGAATGCTGAACCATCACTAAAG A A A S E S : H K K K K P R N A E P S L K Q Q L R K : V I R R K N L G M L N H H - S C S S F G K : S - E E K T - E C - T I T K . . . : . . . 21084 CATGAAATGGATGCTTCACCTAAAAG : CAAGTCAAAAGAGGCGACAGCTAAATCTGGACAG H E M D A S P K S : K S K E A T A K S G Q M K W M L H L K : A S Q K R R Q L N L D R A - N G C F T - K : Q V K R G D S - I W T . . . . . . 20929 AAATCCAAGGGTAAACTCAACTTGAAAGATGGCACCTCAAAATCTGCCGGGAAAGCTGAT K S K G K L N L K D G T S K S A G K A D N P R V N S T - K M A P Q N L P G K L M E I Q G - T Q L E R W H L K I C R E S - . . . . . . 20869 GACACAACCAGCAGCAAGTCTGCGGCTCCATCCAAAAGGAGCACTGGCAAATCAGTTGAT D T T S S K S A A P S K R S T G K S V D T Q P A A S L R L H P K G A L A N Q L I - H N Q Q Q V C G S I Q K E H W Q I S - . . . . . . 20809 ACTGAAAAGCCATCTGCCAGGTCTAAAGATGTTAGTAGTAGTACTCCCAAATCCAAGTCC T E K P S A R S K D V S S S T P K S K S L K S H L P G L K M L V V V L P N P S P Y - K A I C Q V - R C - - - Y S Q I Q V . . . . . . 20749 AGGCAAGATACTCCATCAACAACTGCCAACAAGTCCAAGCAAGAAACAGTTAAAGCTGCC R Q D T P S T T A N K S K Q E T V K A A G K I L H Q Q L P T S P S K K Q L K L P Q A R Y S I N N C Q Q V Q A R N S - S C . . . . . . 20689 AACAAGTCCAAAACCAAAACCCCCCAAAGTGGTGGCAAGTCCGGTGCCAATGGTTCGGAG N K S K T K T P Q S G G K S G A N G S E T S P K P K P P K V V A S P V P M V R R Q Q V Q N Q N P P K W W Q V R C Q W F G . . . . . . 20629 AAGCTAAAATCTAGTTCATCAAAGGTTAAAGAGAGTGGGAACCAGAAGGAAAAAGCAACT K L K S S S S K V K E S G N Q K E K A T S - N L V H Q R L K R V G T R R K K Q L E A K I - F I K G - R E W E P E G K S N . . . 20569 AACTCAGCAAAAACCCCTGATGGCAGCAC N S A K T P D G S T Q Q K P L M A A - L S K N P - W Q H Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-4-_PGL-3_AGS-2_PPS_1 (24211 23369,23239 23126,22953 22825,22364 22291,22131 22080,21992 21906,21833 21796,21127 21059,20963 20543) (frame '1'; 1827 bp, 609 residues) 1 TAESGRDEAC LEDIEPAVNG SPKSITSNGG SLENVRLTSE TESLMKAGAH DEVDLHDASK 61 IPSESDDSRV EKSTKSEPKS MKSEPKSEQP SKKRGRKTVS SINSAESSHQ APEGSGKEIE 121 KLQDHQNDQN KDDHSSASED PVVEQSNLLE KEPETNQHSA PKESEEEVVD VAPPSQGQSL 181 PEEIALKKGD LPMEDNSNQD GESKKEIEAG SDLEVKQVRR PLRKTPLEPC RKEKGGSTSD 241 TEAKKLKQSG KNVDTKNKSQ VGPSARNKED SKKRGHGKAS QETLPSQESP DRSVKHDEDN 301 EEEIPRTTTK RKRSSSKGRG SRQVVQKSVP TPESPDNSTK HISDEDETDT SAKKKPSSGN 361 DRVTETVQCD KNLVGRKIRV WWPLDELFYE GTVSNYDSSR KKFTVDYTDG ETEKLNLLKE 421 RWELVEDDNM SEEEQVASAD AAASESHKKK KPRNAEPSLK HEMDASPKSK SKEATAKSGQ 481 KSKGKLNLKD GTSKSAGKAD DTTSSKSAAP SKRSTGKSVD TEKPSARSKD VSSSTPKSKS 541 RQDTPSTTAN KSKQETVKAA NKSKTKTPQS GGKSGANGSE KLKSSSSKVK ESGNQKEKAT 601 NSAKTPDGS ... finished at: Mon Aug 28 21:42:01 2006 ________________________________________________________________________________ Sequence 5: C06HBa0046G10.1-5, from 1 to 19191, both strands analyzed. ... started at: Mon Aug 28 21:42:01 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 5 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 7 ... matches indexed, elapsed seconds = 7 HitsTableSize = 3 ******************************************************************************** EST sequence 4 -strand 623 n (File: SGN-E342700-) 1 AACCCTAGAG CTGTAATACA CAACCAAGAT CTAAACCGAA ACCCCATTGG CTAAAAAATT 61 TGCAAACTTT AAGCTAAACC ACAAAATGGG GGTGCCATTA TCTTCAGCAA GAAGAAAGAA 121 AAATCAAATC TTTCCTCAAA CTAACTTAAA GGGTGGTTCA GATCTTGATT TACAAATAGG 181 GAAAAGATTG AAAAAAAATG CAAGAATCAT GCAAAGCTGA AGCCTTTTTT GCTCAATTTA 241 CAACCTTGGA TTCTTTAAAT AAAAAGAGAG AGAGCCCAGA TCTCATACAC ATTCATTATA 301 ACTATTTATA TATACATAAC ATGAAAAAGA TTCAGATATA TTGCAGAGAA GGGGGTAGGG 361 AGGGTGTCAG GATGTGGGTA GGTTGGGTGG GTGGTGGGTT GAAGAGGGGA GAAAGAAGGA 421 GATTCAGATG CTGATAACAA GGCTAAAGAA AACAAAAAAT GTCATTATTC ATCATTCAAT 481 TTCTTCATGC ACTAGTACTA TGTACATACA TTTTATACAT AAACATAAAA ACAACAAGTG 541 AATAAAGCTT TCTTCTTCAT TAGGAAATTT TTGGGTTTGA AGGGGGTGGG GTAAGTGAGC 601 TTAATTATGA ACCTTGGCAT GGA Predicted gene structure (within gDNA segment 3159 to 1327): Exon 1 2549 1927 ( 623 n); cDNA 1 623 ( 623 n); score: 0.997 MATCH C06HBa0046G10.1-5- SGN-E342700- 0.997 623 1.000 C PGS_C06HBa0046G10.1-5-_SGN-E342700- (2549 1927) Alignment (genomic DNA sequence = upper lines): AACCCTAGAG CTGTAATACA CAACCAAGAT CTAAACCAAA ACCCCATTGG CTAAAAAATC 2490 |||||||||| |||||||||| |||||||||| ||||||| || |||||||||| ||||||||| AACCCTAGAG CTGTAATACA CAACCAAGAT CTAAACCGAA ACCCCATTGG CTAAAAAATT 60 TGCAAACTTT AAGCTAAACC ACAAAATGGG GGTGCCATTA TCTTCAGCAA GAAGAAAGAA 2430 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCAAACTTT AAGCTAAACC ACAAAATGGG GGTGCCATTA TCTTCAGCAA GAAGAAAGAA 120 AAATCAAATC TTTCCTCAAA CTAACTTAAA GGGTGGTTCA GATCTTGATT TACAAATAGG 2370 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAATCAAATC TTTCCTCAAA CTAACTTAAA GGGTGGTTCA GATCTTGATT TACAAATAGG 180 GAAAAGATTG AAAAAAAATG CAAGAATCAT GCAAAGCTGA AGCCTTTTTT GCTCAATTTA 2310 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAAAGATTG AAAAAAAATG CAAGAATCAT GCAAAGCTGA AGCCTTTTTT GCTCAATTTA 240 CAACCTTGGA TTCTTTAAAT AAAAAGAGAG AGAGCCCAGA TCTCATACAC ATTCATTATA 2250 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAACCTTGGA TTCTTTAAAT AAAAAGAGAG AGAGCCCAGA TCTCATACAC ATTCATTATA 300 ACTATTTATA TATACATAAC ATGAAAAAGA TTCAGATATA TTGCAGAGAA GGGGGTAGGG 2190 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTATTTATA TATACATAAC ATGAAAAAGA TTCAGATATA TTGCAGAGAA GGGGGTAGGG 360 AGGGTGTCAG GATGTGGGTA GGTTGGGTGG GTGGTGGGTT GAAGAGGGGA GAAAGAAGGA 2130 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGGTGTCAG GATGTGGGTA GGTTGGGTGG GTGGTGGGTT GAAGAGGGGA GAAAGAAGGA 420 GATTCAGATG CTGATAACAA GGCTAAAGAA AACAAAAAAT GTCATTATTC ATCATTCAAT 2070 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATTCAGATG CTGATAACAA GGCTAAAGAA AACAAAAAAT GTCATTATTC ATCATTCAAT 480 TTCTTCATGC ACTAGTACTA TGTACATACA TTTTATACAT AAACATAAAA ACAACAAGTG 2010 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCTTCATGC ACTAGTACTA TGTACATACA TTTTATACAT AAACATAAAA ACAACAAGTG 540 AATAAAGCTT TCTTCTTCAT TAGGAAATTT TTGGGTTTGA AGGGGGTGGG GTAAGTGAGC 1950 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATAAAGCTT TCTTCTTCAT TAGGAAATTT TTGGGTTTGA AGGGGGTGGG GTAAGTGAGC 600 TTAATTATGA ACCTTGGCAT GGA 1927 |||||||||| |||||||||| ||| TTAATTATGA ACCTTGGCAT GGA 623 hqPGS_C06HBa0046G10.1-5-_SGN-E342700- (2549 1927) ******************************************************************************** EST sequence 2 +strand 682 n (File: SGN-E318334+) 1 AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 61 CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 121 TAGTTACCAT TTTCAGGTTG CTGAAAGGGC TCTCTTCCTT TGGAATAATG ACCAAATTGT 181 CAATTTGATT GCACATAACC GGCACGTGAT TCTCCCGATT ATATTCCCAG CTGTGGAAAG 241 CAATACTCAG AACCACTGGA ATCATGCTGT GCAGAACTTG AGTCTAAATG TAAGAAGGAT 301 GTTCTCCGAA ATGGATGATG TACTTTTCCT TGCTTGTCAT TCTCATTACA AGGAAGAACA 361 GGAGAAAATA GACTTGGAAT CTGAAAAACG GAAGGAAGCA TGGGAACAGC TGGAGACTGC 421 AGCTAGTCTA CGTCCACCTG TAGCTGGAAA CATTGCTGTC CTGGTAACGC CTTTAGCAAC 481 TTCAATCACT TGCTAAGAGG TGATGACTCC ACTCTATCCT TGTCCCCGTG TTTTTCTGTG 541 TATGTTCCAC GCAATCTAGG AATCATGGAT GGAAAATGGC TGTTGTCTGG AGCAGGTTTT 601 TGNAGATCAA CCCGATTAAC AGCCTTATCT CTGTTGTACC TTAGTCCTGC AACTGTGTTG 661 AGAGCGCGAT GGGGGGGGGG GG Predicted gene structure (within gDNA segment 3048 to 6555): Exon 1 3648 3783 ( 136 n); cDNA 1 136 ( 136 n); score: 1.000 Intron 1 3784 5281 (1498 n); Pd: 0.998 (s: 1.00), Pa: 0.795 (s: 1.00) Exon 2 5282 5829 ( 548 n); cDNA 137 682 ( 546 n); score: 0.995 MATCH C06HBa0046G10.1-5+ SGN-E318334+ 0.996 684 1.003 C PGS_C06HBa0046G10.1-5+_SGN-E318334+ (3648 3783,5282 5829) Alignment (genomic DNA sequence = upper lines): AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 3707 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 60 CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 3767 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 120 TAGTTACCAT TTTCAGGTAC TAATACTTGT GTCTCTAGAC ATGTATATAT GTGTGCGTGT 3827 |||||||||| |||||| TAGTTACCAT TTTCAG.... .......... .......... .......... .......... 136 GTACTTCAAT TTTTATTTGT TGCGTAGTTT CTGAGTTGTT GGTTAGATAT GGTTTGTTCA 3887 .......... .......... .......... .......... .......... .......... 136 AGGATCTATG AACTAATATT GTGATAAGAA TTCTCCTTCT AATTGATACA GAGGATTCAG 3947 .......... .......... .......... .......... .......... .......... 136 GCTAATGTGG GATGGAAATG CAATTGATTG AATGATGTAT TCTTCATCCA GTTGTTTGAC 4007 .......... .......... .......... .......... .......... .......... 136 TTGTAAAGAA TCAGGATCCT TCCAGATTTA CATGTATTTT TAGCTGTGTG GAATTTTTAT 4067 .......... .......... .......... .......... .......... .......... 136 ATGAAAATCT TTAAAATTAC TACCAGCTCA GTTCCCTAGA ATAAAATTTC TAGATTGCTG 4127 .......... .......... .......... .......... .......... .......... 136 ACAGGAGTTT TTCATTTAGT TTGAAATGGT CTCAGCAATG AGTCAAGAAC TTAAGATTAT 4187 .......... .......... .......... .......... .......... .......... 136 GAGCCAAAAA GACTTGTGCC CCAAGAGAAT GTTCAGGATT TTTTTTGTCT ATACGAAACA 4247 .......... .......... .......... .......... .......... .......... 136 ACAGCCTTGT CAATGAACTG GGTAAACAAG TGGTTTCAAG GGATGACAAT AGATAGAAGA 4307 .......... .......... .......... .......... .......... .......... 136 GTGCTGATAA AGAAGATCTA TTACTGTTTC CCAGGGCATT TCTGGAATCA AGTTAATAAA 4367 .......... .......... .......... .......... .......... .......... 136 ATTCATCAGA AAACCTGCCA ATAAGAAGAA TGAGATATAT TGCTTAATCT GCATTATTCA 4427 .......... .......... .......... .......... .......... .......... 136 GTCAATCATT TAAGTATCTT TGTCCATTCT TAGACAGACT CAAAAAATGG ATTTTCAAAG 4487 .......... .......... .......... .......... .......... .......... 136 ACAGAGAGAA ACTGATGAAG TAATTGGATC TTTGACAGAG TTAACTTAAA TAAAACCAAG 4547 .......... .......... .......... .......... .......... .......... 136 TATCATGGGC CTGTTTGGCC AAATCTTCTG ACATCAATCA GTCATTGCAC TTCGATGAAC 4607 .......... .......... .......... .......... .......... .......... 136 GCCACCACCA TATTCTGAAG AGTTGGTGGT GGTGGTTATA GTATGATATC TTTAAACCTG 4667 .......... .......... .......... .......... .......... .......... 136 CAAGTTCTCA ACACATCTAA ACATGTGTAA ATTTCTACTT GTTATAAACC AAGTCTTTCT 4727 .......... .......... .......... .......... .......... .......... 136 GGTAGATACT TGGATTTCAA TCATTATATG GCATCACATG GTTCTTCTCC GTTTATCTTT 4787 .......... .......... .......... .......... .......... .......... 136 TATTGTTGAG GCTGGAAATT GTTGGGTTCA GGGACACAAT TTGCCAAAAA CTATAGCATC 4847 .......... .......... .......... .......... .......... .......... 136 TCACTTCCAA GGCATATAGT ATATTTTTGC GACTAAGTAA TGATCTTCTA ACCAAGAGTG 4907 .......... .......... .......... .......... .......... .......... 136 AGGACTACGC TTATGAATTG TGTATTTATC TAAATACTGG AGGCTTCTAC ATGAAAAGTG 4967 .......... .......... .......... .......... .......... .......... 136 GTTTGTGGAT GGGGAAGAGA GAGAGAGAGA AGAATGAGTA TATGGCAAAA TGGTTGAAAA 5027 .......... .......... .......... .......... .......... .......... 136 ACAAATTTAG TTCATTGTTG GTTGGTTTCT GAAACCCCTT CCTCCTCTTT ATCTTTCCAT 5087 .......... .......... .......... .......... .......... .......... 136 TCACTCTAGA GTTCTCTCTC AACCCCCCTT TTTTCCCAGT GGGGAGTGAT AGATATGAAC 5147 .......... .......... .......... .......... .......... .......... 136 TTCAGGCCTT CTCTGGTAGA AAGGTTGCCA TATGTGATGA TCTCTTTGAT GCTGCATTAG 5207 .......... .......... .......... .......... .......... .......... 136 TCTGTTATCA GTTACCCCCC TCCCTCCCAA AGACATTTAC TTGTCATCCT AACAGTCAAA 5267 .......... .......... .......... .......... .......... .......... 136 CTTCTATTTT CCAGGTTGCT GAAAGGGCTC TCTTCCTTTG GAATAATGAC CAAATTGTCA 5327 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....GTTGCT GAAAGGGCTC TCTTCCTTTG GAATAATGAC CAAATTGTCA 182 ATTTGATTGC ACATAACCGG CACGTGATTC TCCCGATTAT ATTCCCAGCT GTGGAAAGCA 5387 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTGATTGC ACATAACCGG CACGTGATTC TCCCGATTAT ATTCCCAGCT GTGGAAAGCA 242 ATACTCAGAA CCACTGGAAT CATGCTGTGC AGAACTTGAG TCTAAATGTA AGAAGGATGT 5447 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATACTCAGAA CCACTGGAAT CATGCTGTGC AGAACTTGAG TCTAAATGTA AGAAGGATGT 302 TCTCCGAAAT GGATGATGTA CTTTTCCTTG CTTGTCATTC TCATTACAAG GAAGAACAGG 5507 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCCGAAAT GGATGATGTA CTTTTCCTTG CTTGTCATTC TCATTACAAG GAAGAACAGG 362 AGAAAATAGA CTTGGAATCT GAAAAACGGA AGGAAGCATG GGAACAGCTG GAGACTGCAG 5567 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAAATAGA CTTGGAATCT GAAAAACGGA AGGAAGCATG GGAACAGCTG GAGACTGCAG 422 CTAGTCTACG TCCACCTGTA GCTGGAAACA TTGCTGTCCT GGTAACGCCT TTAGCAACTT 5627 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTAGTCTACG TCCACCTGTA GCTGGAAACA TTGCTGTCCT GGTAACGCCT TTAGCAACTT 482 CAATCACTTG CTAAGAGGTG ATGACTCCAC TCTATCCTTG TCCCCGTGTT TTTCTGTGTA 5687 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAATCACTTG CTAAGAGGTG ATGACTCCAC TCTATCCTTG TCCCCGTGTT TTTCTGTGTA 542 TGTTCCACGC AATCTAGGAA TCATGGATGG AAAATGGCTG TTGTCTGGAG CAGGTTTTTG 5747 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTCCACGC AATCTAGGAA TCATGGATGG AAAATGGCTG TTGTCTGGAG CAGGTTTTTG 602 AAGATCAACC CGATTAACAG CCTTATCTCT GTTGTAACCT TAGTCCTGCA ACTGTGTTGA 5807 ||||||||| |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| NAGATCAACC CGATTAACAG CCTTATCTCT GTTGT-ACCT TAGTCCTGCA ACTGTGTTGA 661 GAGCGCGAAT GGGGGGGGGG GG 5829 ||||||| || |||||||||| || GAGCGCG-AT GGGGGGGGGG GG 682 hqPGS_C06HBa0046G10.1-5+_SGN-E318334+ (3648 3783,5282 5829) ******************************************************************************** EST sequence 3 +strand 682 n (File: SGN-E318159+) 1 AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 61 CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 121 TAGTTACCAT TTTCAGGTTG CTGAAAGGGC TCTCTTCCTT TGGAATAATG ACCAAATTGT 181 CAATTTGATT GCACATAACC GGCACGTGAT TCTCCCGATT ATATTCCCAG CTGTGGAAAG 241 CAATACTCAG AACCACTGGA ATCATGCTGT GCAGAACTTG AGTCTAAATG TAAGAAGGAT 301 GTTCTCCGAA ATGGATGATG TACTTTTCCT TGCTTGTCAT TCTCATTACA AGGAAGAACA 361 GGAGAAAATA GACTTGGAAT CTGAAAAACG GAAGGAAGCA TGGGAACAGC TGGAGACTGC 421 AGCTAGTCTA CGTCCACCTG TAGCTGGAAA CATTGCTGTC CTGGTAACGC CTTTAGCAAC 481 TTCAATCACT TGCTAAGAGG TGATGACTCC ACTCTATCCT TGTCCCCGTG TTTTTCTGTG 541 TATGTTCCAC GCAATCTAGG AATCATGGAT GGAAAATGGC TGTTGTCTGG AGCAGGTTTT 601 TGAAGATCAA CCCGATTAAC AGCCTTATCT CTGTTGTAAC CTTAGTCCTG CAACTGTGTT 661 GAGACGCNAA TGGGGGGGGG GG Predicted gene structure (within gDNA segment 3048 to 6599): Exon 1 3648 3783 ( 136 n); cDNA 1 136 ( 136 n); score: 1.000 Intron 1 3784 5281 (1498 n); Pd: 0.998 (s: 1.00), Pa: 0.795 (s: 1.00) Exon 2 5282 5828 ( 547 n); cDNA 137 682 ( 546 n); score: 0.996 MATCH C06HBa0046G10.1-5+ SGN-E318159+ 0.997 683 1.001 C PGS_C06HBa0046G10.1-5+_SGN-E318159+ (3648 3783,5282 5828) Alignment (genomic DNA sequence = upper lines): AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 3707 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCACCCAA AAGGAGGTGA TGTTTTTGAG TGAGCTGGAA GAAATTTTGG AAGTTATTAA 60 CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 3767 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATGGCTGAG TTCCAAAAAG TGATGGTTCC CTTGTTCTGG CGGATCGGTT GCTGCATCAA 120 TAGTTACCAT TTTCAGGTAC TAATACTTGT GTCTCTAGAC ATGTATATAT GTGTGCGTGT 3827 |||||||||| |||||| TAGTTACCAT TTTCAG.... .......... .......... .......... .......... 136 GTACTTCAAT TTTTATTTGT TGCGTAGTTT CTGAGTTGTT GGTTAGATAT GGTTTGTTCA 3887 .......... .......... .......... .......... .......... .......... 136 AGGATCTATG AACTAATATT GTGATAAGAA TTCTCCTTCT AATTGATACA GAGGATTCAG 3947 .......... .......... .......... .......... .......... .......... 136 GCTAATGTGG GATGGAAATG CAATTGATTG AATGATGTAT TCTTCATCCA GTTGTTTGAC 4007 .......... .......... .......... .......... .......... .......... 136 TTGTAAAGAA TCAGGATCCT TCCAGATTTA CATGTATTTT TAGCTGTGTG GAATTTTTAT 4067 .......... .......... .......... .......... .......... .......... 136 ATGAAAATCT TTAAAATTAC TACCAGCTCA GTTCCCTAGA ATAAAATTTC TAGATTGCTG 4127 .......... .......... .......... .......... .......... .......... 136 ACAGGAGTTT TTCATTTAGT TTGAAATGGT CTCAGCAATG AGTCAAGAAC TTAAGATTAT 4187 .......... .......... .......... .......... .......... .......... 136 GAGCCAAAAA GACTTGTGCC CCAAGAGAAT GTTCAGGATT TTTTTTGTCT ATACGAAACA 4247 .......... .......... .......... .......... .......... .......... 136 ACAGCCTTGT CAATGAACTG GGTAAACAAG TGGTTTCAAG GGATGACAAT AGATAGAAGA 4307 .......... .......... .......... .......... .......... .......... 136 GTGCTGATAA AGAAGATCTA TTACTGTTTC CCAGGGCATT TCTGGAATCA AGTTAATAAA 4367 .......... .......... .......... .......... .......... .......... 136 ATTCATCAGA AAACCTGCCA ATAAGAAGAA TGAGATATAT TGCTTAATCT GCATTATTCA 4427 .......... .......... .......... .......... .......... .......... 136 GTCAATCATT TAAGTATCTT TGTCCATTCT TAGACAGACT CAAAAAATGG ATTTTCAAAG 4487 .......... .......... .......... .......... .......... .......... 136 ACAGAGAGAA ACTGATGAAG TAATTGGATC TTTGACAGAG TTAACTTAAA TAAAACCAAG 4547 .......... .......... .......... .......... .......... .......... 136 TATCATGGGC CTGTTTGGCC AAATCTTCTG ACATCAATCA GTCATTGCAC TTCGATGAAC 4607 .......... .......... .......... .......... .......... .......... 136 GCCACCACCA TATTCTGAAG AGTTGGTGGT GGTGGTTATA GTATGATATC TTTAAACCTG 4667 .......... .......... .......... .......... .......... .......... 136 CAAGTTCTCA ACACATCTAA ACATGTGTAA ATTTCTACTT GTTATAAACC AAGTCTTTCT 4727 .......... .......... .......... .......... .......... .......... 136 GGTAGATACT TGGATTTCAA TCATTATATG GCATCACATG GTTCTTCTCC GTTTATCTTT 4787 .......... .......... .......... .......... .......... .......... 136 TATTGTTGAG GCTGGAAATT GTTGGGTTCA GGGACACAAT TTGCCAAAAA CTATAGCATC 4847 .......... .......... .......... .......... .......... .......... 136 TCACTTCCAA GGCATATAGT ATATTTTTGC GACTAAGTAA TGATCTTCTA ACCAAGAGTG 4907 .......... .......... .......... .......... .......... .......... 136 AGGACTACGC TTATGAATTG TGTATTTATC TAAATACTGG AGGCTTCTAC ATGAAAAGTG 4967 .......... .......... .......... .......... .......... .......... 136 GTTTGTGGAT GGGGAAGAGA GAGAGAGAGA AGAATGAGTA TATGGCAAAA TGGTTGAAAA 5027 .......... .......... .......... .......... .......... .......... 136 ACAAATTTAG TTCATTGTTG GTTGGTTTCT GAAACCCCTT CCTCCTCTTT ATCTTTCCAT 5087 .......... .......... .......... .......... .......... .......... 136 TCACTCTAGA GTTCTCTCTC AACCCCCCTT TTTTCCCAGT GGGGAGTGAT AGATATGAAC 5147 .......... .......... .......... .......... .......... .......... 136 TTCAGGCCTT CTCTGGTAGA AAGGTTGCCA TATGTGATGA TCTCTTTGAT GCTGCATTAG 5207 .......... .......... .......... .......... .......... .......... 136 TCTGTTATCA GTTACCCCCC TCCCTCCCAA AGACATTTAC TTGTCATCCT AACAGTCAAA 5267 .......... .......... .......... .......... .......... .......... 136 CTTCTATTTT CCAGGTTGCT GAAAGGGCTC TCTTCCTTTG GAATAATGAC CAAATTGTCA 5327 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....GTTGCT GAAAGGGCTC TCTTCCTTTG GAATAATGAC CAAATTGTCA 182 ATTTGATTGC ACATAACCGG CACGTGATTC TCCCGATTAT ATTCCCAGCT GTGGAAAGCA 5387 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTGATTGC ACATAACCGG CACGTGATTC TCCCGATTAT ATTCCCAGCT GTGGAAAGCA 242 ATACTCAGAA CCACTGGAAT CATGCTGTGC AGAACTTGAG TCTAAATGTA AGAAGGATGT 5447 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATACTCAGAA CCACTGGAAT CATGCTGTGC AGAACTTGAG TCTAAATGTA AGAAGGATGT 302 TCTCCGAAAT GGATGATGTA CTTTTCCTTG CTTGTCATTC TCATTACAAG GAAGAACAGG 5507 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCCGAAAT GGATGATGTA CTTTTCCTTG CTTGTCATTC TCATTACAAG GAAGAACAGG 362 AGAAAATAGA CTTGGAATCT GAAAAACGGA AGGAAGCATG GGAACAGCTG GAGACTGCAG 5567 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAAATAGA CTTGGAATCT GAAAAACGGA AGGAAGCATG GGAACAGCTG GAGACTGCAG 422 CTAGTCTACG TCCACCTGTA GCTGGAAACA TTGCTGTCCT GGTAACGCCT TTAGCAACTT 5627 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTAGTCTACG TCCACCTGTA GCTGGAAACA TTGCTGTCCT GGTAACGCCT TTAGCAACTT 482 CAATCACTTG CTAAGAGGTG ATGACTCCAC TCTATCCTTG TCCCCGTGTT TTTCTGTGTA 5687 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAATCACTTG CTAAGAGGTG ATGACTCCAC TCTATCCTTG TCCCCGTGTT TTTCTGTGTA 542 TGTTCCACGC AATCTAGGAA TCATGGATGG AAAATGGCTG TTGTCTGGAG CAGGTTTTTG 5747 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTCCACGC AATCTAGGAA TCATGGATGG AAAATGGCTG TTGTCTGGAG CAGGTTTTTG 602 AAGATCAACC CGATTAACAG CCTTATCTCT GTTGTAACCT TAGTCCTGCA ACTGTGTTGA 5807 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGATCAACC CGATTAACAG CCTTATCTCT GTTGTAACCT TAGTCCTGCA ACTGTGTTGA 662 GAGCGCGAAT GGGGGGGGGG G 5828 || ||| ||| |||||||||| | GA-CGCNAAT GGGGGGGGGG G 682 hqPGS_C06HBa0046G10.1-5+_SGN-E318159+ (3648 3783,5282 5828) ******************************************************************************** EST sequence 1 +strand 631 n (File: SGN-E231056+) 1 AAACAGCCAT CAAACTCCTG TGAAAACCCC ACCTCTTTCT CGATTTTCCT CACCACTAAA 61 CAACCACGAA AGATCACCCA TTCTTTCCCC ACCAACGAGC TAACAACAGC CCCATTCCGG 121 CAAAACAACA AGCTGCGGCG AAGCTACTAG ACCATATCTC GTACAAACGA GCTCGGAGCT 181 TTTGTCCCAA CAACATTGTC ATCTTCAAAT CTGCTTGTGA AAGTAACGAC ATGGGAAAAT 241 ACTACACAAG ATGAGCCCAT TGATATTGTA AATATCGATG ATCACGACCT TCCTAATTTA 301 CGGCTCACCT TCAGCTAGTG ATAGTGATGA TTTGCCTAAT GCTGAGGAAT CAGGAGATAA 361 TGTTCCATTT GAGGCATCAC AGTGATGATT TTTTGACTCC CAATTGATCA CCAAGACCAA 421 TGTCCATGAG TCTGTTTCGT AATCGTGAAA TTTCTATCTT GATCATATCT CGGATGGTCC 481 AGATATCTTT GGTGATACAC ATGATAAATA TACATCACAA CGTACGTTCC ATGAACCAGA 541 AGATTTCATG AATGATGCTA TTTATATTGA AAAAGTTACT CAATTCAAAG AAGTAGTTGC 601 AAAGAGCAGT TAAACTTCTA CATTTGAACA T Predicted gene structure (within gDNA segment 4391 to 9411): Exon 1 7900 8302 ( 403 n); cDNA 236 631 ( 396 n); score: 0.885 MATCH C06HBa0046G10.1-5+ SGN-E231056+ 0.885 403 0.639 C PGS_C06HBa0046G10.1-5+_SGN-E231056+ (7900 8302) Alignment (genomic DNA sequence = upper lines): AAAATATTAC ACAAGATGAG CCAATTGATC CTGTGAATAT CAATAATCGT GATCTTCCTA 7959 |||||| ||| |||||||||| || |||||| ||| ||||| | || ||| || ||||||| AAAATACTAC ACAAGATGAG CCCATTGATA TTGTAAATAT CGATGATCAC GACCTTCCTA 295 A-TTACGGCT CACCTTCAGC TAGTAATAGT GATGATTTGC CTAATGCTGA GGAATCAGGA 8018 | |||||||| |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| ATTTACGGCT CACCTTCAGC TAGTGATAGT GATGATTTGC CTAATGCTGA GGAATCAGGA 355 GATAATGTTC CATTTGAGGC ATCATCTAGT GATGATGATT TTGACTCCCA TTCGATCGCC 8078 |||||||||| |||||||||| |||| | ||| |||||| || |||||||||| | |||| || GATAATGTTC CATTTGAGGC ATCA-C-AGT GATGAT-TTT TTGACTCCCA ATTGATCACC 412 AAGACCAATG TCCATGAGTC CGTTTCGTAA TCATTAATTC CTTATCTTGA TCATCTCCCG 8138 |||||||||| |||||||||| ||||||||| || | || | |||||||| |||| || || AAGACCAATG TCCATGAGTC TGTTTCGTAA TCGTGAAATT TCTATCTTGA TCATATCTCG 472 GATAGTCCAT ATATCTTTGG TGATACACAT GATGAGTATA CATCACAACG TACGTGGCAT 8198 ||| ||||| |||||||||| |||||||||| ||| | |||| |||||||||| ||||| ||| GATGGTCCAG ATATCTTTGG TGATACACAT GATAAATATA CATCACAACG TACGTTCCAT 532 GAACCAGAAG ATTTCATGAA TGATGCTATT TATATTGAAA AAGACATGTT ATTCAATTCA 8258 |||||||||| |||||||||| |||||||||| ||||||| | || | | ||| | |||||||| GAACCAGAAG ATTTCATGAA TGATGCTATT TATATTG--A AA-A-A-GTT ACTCAATTCA 587 AATAAGCAGT TGCAAAGAGC AGTAAAACTT TTACATTTGA AGAT 8302 || ||| ||| |||||||||| ||| |||||| ||||||||| | || AAGAAGTAGT TGCAAAGAGC AGTTAAACTT CTACATTTGA ACAT 631 hqPGS_C06HBa0046G10.1-5+_SGN-E231056+ (7900 8302) ******************************************************************************** EST sequence 5 +strand 475 n (File: SGN-E240778+) 1 AAACAGCCAT CAAACTCCTG TGAAAACCCC ACCTCTTTCT CGATTTTCCT CACCACTAAA 61 CAACCACGAA AGATCACCCA TTCTTTCCCC ACCAACGAGC TAACAACAGC CCCATTCCGG 121 CAAAACAACA AGCTGCGGCG AAGCTACTAG ACCATATCTC GTACAAACGA GCTCGGAGCT 181 TTTGTCCCAA CAACATTGTC ATCTTCAAAT CTGCTTGTGA AAGTAACGAC ATGGGAAAAT 241 ACTACACAAG ATGAGCCCAT TGATATTGTA AATATCGATG ATCACGACCT TCCTAATTTA 301 CGGCTCACCT TCAGCTAGTG ATAGTGATGA TTTGCCTAAT GCTGAGGAAT CAGGAGATAA 361 TGTTCCATTT GAGGCATCAC AGTGATGATT TTTTGACTCC CAATTGATCA CCAAGACCAA 421 TGTCCATGAG TCTGTTTCGT AATCGTGAAA TTTCTATCTT GATCATATCT CGGAT Predicted gene structure (within gDNA segment 4391 to 9612): Exon 1 7900 8141 ( 242 n); cDNA 236 475 ( 240 n); score: 0.878 MATCH C06HBa0046G10.1-5+ SGN-E240778+ 0.878 242 0.509 C PGS_C06HBa0046G10.1-5+_SGN-E240778+ (7900 8141) Alignment (genomic DNA sequence = upper lines): AAAATATTAC ACAAGATGAG CCAATTGATC CTGTGAATAT CAATAATCGT GATCTTCCTA 7959 |||||| ||| |||||||||| || |||||| ||| ||||| | || ||| || ||||||| AAAATACTAC ACAAGATGAG CCCATTGATA TTGTAAATAT CGATGATCAC GACCTTCCTA 295 A-TTACGGCT CACCTTCAGC TAGTAATAGT GATGATTTGC CTAATGCTGA GGAATCAGGA 8018 | |||||||| |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| ATTTACGGCT CACCTTCAGC TAGTGATAGT GATGATTTGC CTAATGCTGA GGAATCAGGA 355 GATAATGTTC CATTTGAGGC ATCATCTAGT GATGATGATT TTGACTCCCA TTCGATCGCC 8078 |||||||||| |||||||||| |||| | ||| |||||| || |||||||||| | |||| || GATAATGTTC CATTTGAGGC ATCA-C-AGT GATGAT-TTT TTGACTCCCA ATTGATCACC 412 AAGACCAATG TCCATGAGTC CGTTTCGTAA TCATTAATTC CTTATCTTGA TCATCTCCCG 8138 |||||||||| |||||||||| ||||||||| || | || | |||||||| |||| || || AAGACCAATG TCCATGAGTC TGTTTCGTAA TCGTGAAATT TCTATCTTGA TCATATCTCG 472 GAT 8141 ||| GAT 475 hqPGS_C06HBa0046G10.1-5+_SGN-E240778+ (7900 8141) ******************************************************************************** EST sequence 8 +strand 452 n (File: SGN-E313671+) 1 TGAGCTTTCC TTTCTCTTTT ATGATATCTT GGATGCATAC TTTTTTCCTA CTTGAGTAAT 61 GATCTGAACA TTTCCCAAAT GTTTTCCTTT GTCTTCTATA TACGACTTGA AGTTCCCTGT 121 GGATGTGAAA AGGCTAATTA CTAATTGCAT ATCCATTGTC AGGTAGTAAG AATGGAAAGT 181 CTGGTTCTGA ATTTTTTGGG CTTTCAACTT GCTGCTCCGA CCACCAAAAA GTTCCTGAGG 241 AGATTCGTTC AAGCATCACA AGCTTCTTAT GAGGTTCCCT CTGTTGAACT GGAATTCATG 301 GCAAACTATT TAGCAGAGTT AACACTAGCT GAATATAGTT TTCTCAAGTT TCTTCCATCT 361 GTTACTGCTG CATCAGCTGT ATTTCTAGCC AGATGGACAC TTGATCAATC TAACCACCCA 421 TGGAATTCAA CTTTGGAGCA CTACACTACA TA Predicted gene structure (within gDNA segment 12495 to 10580): Exon 1 11895 11657 ( 239 n); cDNA 1 239 ( 239 n); score: 1.000 Intron 1 11656 11570 ( 87 n); Pd: 0.967 (s: 1.00), Pa: 0.893 (s: 0) Exon 2 11569 11536 ( 34 n); cDNA 240 273 ( 34 n); score: 1.000 Intron 2 11535 11454 ( 82 n); Pd: 0.785 (s: 0), Pa: 0.965 (s: 1.00) Exon 3 11453 11304 ( 150 n); cDNA 274 423 ( 150 n); score: 1.000 Intron 3 11303 11219 ( 85 n); Pd: 0.997 (s: 1.00), Pa: 0.949 (s: 0) Exon 4 11218 11190 ( 29 n); cDNA 424 452 ( 29 n); score: 1.000 MATCH C06HBa0046G10.1-5- SGN-E313671+ 1.000 452 1.000 C PGS_C06HBa0046G10.1-5-_SGN-E313671+ (11895 11657,11569 11536,11453 11304,11218 11190) Alignment (genomic DNA sequence = upper lines): TGAGCTTTCC TTTCTCTTTT ATGATATCTT GGATGCATAC TTTTTTCCTA CTTGAGTAAT 11836 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAGCTTTCC TTTCTCTTTT ATGATATCTT GGATGCATAC TTTTTTCCTA CTTGAGTAAT 60 GATCTGAACA TTTCCCAAAT GTTTTCCTTT GTCTTCTATA TACGACTTGA AGTTCCCTGT 11776 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATCTGAACA TTTCCCAAAT GTTTTCCTTT GTCTTCTATA TACGACTTGA AGTTCCCTGT 120 GGATGTGAAA AGGCTAATTA CTAATTGCAT ATCCATTGTC AGGTAGTAAG AATGGAAAGT 11716 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGATGTGAAA AGGCTAATTA CTAATTGCAT ATCCATTGTC AGGTAGTAAG AATGGAAAGT 180 CTGGTTCTGA ATTTTTTGGG CTTTCAACTT GCTGCTCCGA CCACCAAAAA GTTCCTGAGG 11656 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| CTGGTTCTGA ATTTTTTGGG CTTTCAACTT GCTGCTCCGA CCACCAAAAA GTTCCTGAG. 239 TACCTGTAAT TAGCAATATA TGCAAATTTA ATCATGTTAC TTGTGAGAGT AGGCTCCTTA 11596 .......... .......... .......... .......... .......... .......... 239 TTTGTGCCAT TGTCCTTTGT TTTTAGGAGA TTCGTTCAAG CATCACAAGC TTCTTATGAG 11536 |||| |||||||||| |||||||||| |||||||||| .......... .......... ......GAGA TTCGTTCAAG CATCACAAGC TTCTTATGAG 273 GTAGATGAGA TATCATTCTT CTTTTGGTTT GGGTCCTAGA AAATGAAGCT TATATGACAA 11476 .......... .......... .......... .......... .......... .......... 273 ATTAAATTTC TCCAACCTAC AGGTTCCCTC TGTTGAACTG GAATTCATGG CAAACTATTT 11416 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..GTTCCCTC TGTTGAACTG GAATTCATGG CAAACTATTT 311 AGCAGAGTTA ACACTAGCTG AATATAGTTT TCTCAAGTTT CTTCCATCTG TTACTGCTGC 11356 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCAGAGTTA ACACTAGCTG AATATAGTTT TCTCAAGTTT CTTCCATCTG TTACTGCTGC 371 ATCAGCTGTA TTTCTAGCCA GATGGACACT TGATCAATCT AACCACCCAT GGGTATGCAA 11296 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || ATCAGCTGTA TTTCTAGCCA GATGGACACT TGATCAATCT AACCACCCAT GG........ 423 CTTTTTTGCA CCTTGATCCT GTCATCTTTT TTTGCAATTG AATGCAACCA CAAAATAACT 11236 .......... .......... .......... .......... .......... .......... 423 CCTGAAATGT CTTGCAGAAT TCAACTTTGG AGCACTACAC TACATA 11190 ||| |||||||||| |||||||||| |||||| .......... .......AAT TCAACTTTGG AGCACTACAC TACATA 452 hqPGS_C06HBa0046G10.1-5-_SGN-E313671+ (11895 11657,11569 11536,11453 11304,11218 11190) ******************************************************************************** EST sequence 7 +strand 229 n (File: SGN-E237597+) 1 TTTTATTTTA TTATTATTTT GCGAGAAAAT GACTATTCTC ATTGACTGCA GCTGCTTGGA 61 GTTACCTGCA TGCTAATTGC TTCCAAATAT GAAGAAATTT GTGCACCTCG TGTGGAAGAA 121 TTTTGCTTTA TTACAGACAA CACTTACTCG AAAGAAGAGG TAGTAAGAAT GGAAAGTCTG 181 GTTCTGAATT TTTTGGGCTT TCAACTTTGC TGGTCCGACC ACCAAAAAA Predicted gene structure (within gDNA segment 13938 to 10856): Exon 1 12891 12860 ( 32 n); cDNA 52 83 ( 32 n); score: 1.000 Intron 1 12859 12535 ( 325 n); Pd: 0.922 (s: 0), Pa: 0.997 (s: 1.00) Exon 2 12534 12459 ( 76 n); cDNA 84 159 ( 76 n); score: 1.000 Intron 2 12458 11734 ( 725 n); Pd: 0.896 (s: 1.00), Pa: 0.710 (s: 0.95) Exon 3 11733 11666 ( 68 n); cDNA 160 228 ( 69 n); score: 0.949 MATCH C06HBa0046G10.1-5- SGN-E237597+ 0.976 176 0.769 C PGS_C06HBa0046G10.1-5-_SGN-E237597+ (12891 12860,12534 12459,11733 11666) Alignment (genomic DNA sequence = upper lines): CTGCTTGGAG TTACCTGCAT GCTAATTGCT TCGTAAGTAA ATCATTTTTG GATATTCATA 12832 |||||||||| |||||||||| |||||||||| || CTGCTTGGAG TTACCTGCAT GCTAATTGCT TC........ .......... .......... 83 CTCAAGGATG TCAGGTTATA GGGTAGACTA AACAGTTTTT ACATTTATAA ACTTGTAGAT 12772 .......... .......... .......... .......... .......... .......... 83 CACAAAGATT TTCCTTTTTT TACCGTGGTA GCTTGTGGGA ACTAGAGCCA TTTAACTAAC 12712 .......... .......... .......... .......... .......... .......... 83 ATTCCCCTTT TTAAAAATTT GTATGTGCAT GTGCTATTCA ACACGGTAAT ATACTTTGCC 12652 .......... .......... .......... .......... .......... .......... 83 CAATTTTTAG AATTATTTGT AGTTTCAGTA AAGAGAAACT ATATGTACTT CCATCTGCAT 12592 .......... .......... .......... .......... .......... .......... 83 CTTTTCATTG TAATTTTTCT GCAGTATGTG ATCTTTTATT TTCTTTTTTG CTTGCAGCAA 12532 ||| .......... .......... .......... .......... .......... .......CAA 86 ATATGAAGAA ATTTGTGCAC CTCGTGTGGA AGAATTTTGC TTTATTACAG ACAACACTTA 12472 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATATGAAGAA ATTTGTGCAC CTCGTGTGGA AGAATTTTGC TTTATTACAG ACAACACTTA 146 CTCGAAAGAA GAGGTATGCT CATTCCAATG CTGATCTAGT CGTGATTTTC TTTTATGTGG 12412 |||||||||| ||| CTCGAAAGAA GAG....... .......... .......... .......... .......... 159 AGAAAGAGGT ACTTGGAGAT ATGTTATGTG ATAAAGAGCA ATTGAATAAC TTTAGTTTCA 12352 .......... .......... .......... .......... .......... .......... 159 ATTAGTGTTA GAAGCATGAT GAGTTGCTTT AGATTCTTTA AATTCATTTC TCCTGGTTGT 12292 .......... .......... .......... .......... .......... .......... 159 ATTTGTCGTT TGTACCCATC CTTGATGTTT GGTCACTCAA TATTGAGCTC CCGTGCTATG 12232 .......... .......... .......... .......... .......... .......... 159 TGATACTCTC TAGATGTCTA GTACTGGGCA TTTAAGGAGT GTTAGAACCC ACAGTGGTTG 12172 .......... .......... .......... .......... .......... .......... 159 AGAGTAAAGG TTGTAGCTCT TATATGCTTT TCAACCATCC TCAGTTCATG AGCGAACTTT 12112 .......... .......... .......... .......... .......... .......... 159 GAAGGTCAAG CCCAAGGTCT GTTATAACAT TTTGTACCTT TATGCATTCA ACTAGTCTTT 12052 .......... .......... .......... .......... .......... .......... 159 CAGAATTTGT CCTTGTAAAC TGATAACTGT ATAAGACCCA CACCTTGCAT TCCTCTGCTA 11992 .......... .......... .......... .......... .......... .......... 159 GTTCTTTTGA CTGTTTTTGC TTGTCCTAGC TACATTACTT TACCCCCTTG TGTTTATCTT 11932 .......... .......... .......... .......... .......... .......... 159 CTTAAAAGCG AAAATCTAGA TTGCCTCATC TAGCTCTGAG CTTTCCTTTC TCTTTTATGA 11872 .......... .......... .......... .......... .......... .......... 159 TATCTTGGAT GCATACTTTT TTCCTACTTG AGTAATGATC TGAACATTTC CCAAATGTTT 11812 .......... .......... .......... .......... .......... .......... 159 TCCTTTGTCT TCTATATACG ACTTGAAGTT CCCTGTGGAT GTGAAAAGGC TAATTACTAA 11752 .......... .......... .......... .......... .......... .......... 159 TTGCATATCC ATTGTCAGGT AGTAAGAATG GAAAGTCTGG TTCTGAATTT TTTGGGCTTT 11692 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........GT AGTAAGAATG GAAAGTCTGG TTCTGAATTT TTTGGGCTTT 201 CAAC-TTGCT GCTCCGACCA CCAAAAA 11666 |||| ||||| | |||||||| ||||||| CAACTTTGCT GGTCCGACCA CCAAAAA 228 hqPGS_C06HBa0046G10.1-5-_SGN-E237597+ (12891 12860,12534 12459,11733 11666) ******************************************************************************** EST sequence 6 +strand 675 n (File: SGN-E314602+) 1 ACCCCTCTGG AACCACACAG ACGAGCTACA TTGATTGTAT GACCGATCGG AGAAGGTAGA 61 AACCCTAGCT CTGCAATCGA GGCACCGCCG TAGAACGGTG TTGCGGGCAG CTCATGAGCT 121 GTTAATAATG CTTAGCTCGT CCCTGATTCA AGAGTACAGT GACAGTTGCT CCTTATTTAT 181 ATAAAGCTCT AGAATTGTGG TGGAAGACAA TGCAGTTGTA GCTCTGACAA TTGGCATATC 241 ACCACCTGGG TCAATGAGGA ATGCAACTAT GGCAACTGGA TCTTCTAATC TTAAAGTGCC 301 TACTATGCGA ATCACAAGAG CACGGGCAAA AACCTTGGGT TCATCAGGAG GACTACCACC 361 TCTACACCCA TCTGTCAGAC AGGATAAGAA ACAGGGACTT GTAACACAAG GAACAAAATC 421 CAAAAGACCA GCCCCAGATG AGAACAAACC AGCTAATTCT TCCAGTACTG CTTCTCAACA 481 GCCTAAGCGA AGGGCTGTTC TCAGGGATGT CACTAATGTG CTTTGTGAAA ATCCGTACAT 541 GAATTGCATC AATGGAAGCA AATTTCAGGT TAAGAAATTC TCTGATAAGA GGAATTCAAA 601 GGTGACACCT GCTCTTTTGG TGAAAAAGCT GAGCTTGAAG ATAGAAAAGA GAGTGTGATT 661 GAAGAAGCAA AAATG Predicted gene structure (within gDNA segment 19191 to 15018): Exon 1 19139 19005 ( 135 n); cDNA 1 134 ( 134 n); score: 0.985 Intron 1 19004 17845 (1160 n); Pd: 0.969 (s: 1.00), Pa: 0.958 (s: 1.00) Exon 2 17844 17764 ( 81 n); cDNA 135 215 ( 81 n); score: 1.000 Intron 2 17763 16278 (1486 n); Pd: 0.927 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 16277 15925 ( 353 n); cDNA 216 568 ( 353 n); score: 1.000 Intron 3 15924 15736 ( 189 n); Pd: 0.988 (s: 1.00), Pa: 0.970 (s: 1.00) Exon 4 15735 15628 ( 108 n); cDNA 569 675 ( 107 n); score: 0.991 MATCH C06HBa0046G10.1-5- SGN-E314602+ 0.996 677 1.003 C PGS_C06HBa0046G10.1-5-_SGN-E314602+ (19139 19005,17844 17764,16277 15925,15735 15628) Alignment (genomic DNA sequence = upper lines): ACCCCTTTTG GAACCACACA GACGAGCTAC ATTGATTGTA TGACCGATCG GAGAAGGTAG 19080 ||||| | || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCCC-TCTG GAACCACACA GACGAGCTAC ATTGATTGTA TGACCGATCG GAGAAGGTAG 59 AAACCCTAGC TCTGCAATCG AGGCACCGCC GTAGAACGGT GTTGCGGGCA GCTCATGAGC 19020 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACCCTAGC TCTGCAATCG AGGCACCGCC GTAGAACGGT GTTGCGGGCA GCTCATGAGC 119 TGTTAATAAT GCTTAGTAAG CTCAGCTTAG ACCTATACAT ACATGTTTGA TATGTACTTG 18960 |||||||||| ||||| TGTTAATAAT GCTTA..... .......... .......... .......... .......... 134 TTTATGTGTA CATATCGATA TGGATATTGT ATTAGCTGCA ATGTATCACA GCCTTGTAGG 18900 .......... .......... .......... .......... .......... .......... 134 TTTTTCTAGA TGAATAAGGG ATTTATTTTG AAATTTTGAA AAATTTTAGC TCTATTTTGA 18840 .......... .......... .......... .......... .......... .......... 134 GTAATCCTTG AGGATTTGCT TATGGATCTG TGAGCTTAGA GTTTTTCTTC CATTTTTCCT 18780 .......... .......... .......... .......... .......... .......... 134 TAATTTCTGG AATCCTGATT TGCTTAATTT CTATTGTTGT TGTTGCTGGT TGTATTTTTT 18720 .......... .......... .......... .......... .......... .......... 134 TGAGTTTTTT TTATGGTTCA TTGTTGTTGG ATTTTGAGGT TCTTTGATTT TCATTTTCTG 18660 .......... .......... .......... .......... .......... .......... 134 GTTACTTATG AAAAAATTGA TTTGGATTAT TTATTTTCAA TTTCTATTTT TGTTCTTAGT 18600 .......... .......... .......... .......... .......... .......... 134 TGTTGTCTTT ACTTTTTTTC TTAAATTTTT TTTTTAGTGC ATTTTGGTTG AATTTTGAGG 18540 .......... .......... .......... .......... .......... .......... 134 TCGGAAAGCT TTTTTTAAAT TATTTTTTTC TAGTTTCTTT TGAGAAAAGA AATTATTTGG 18480 .......... .......... .......... .......... .......... .......... 134 TTTTGTTAAT TGCCATTTTC AGTTTCTTAT ACTTAGTTGT GATTAAGCAA ATTAAATTTT 18420 .......... .......... .......... .......... .......... .......... 134 GATGTGTTTT GGTTGATTCT TTGAGTTCTA AAGCTATTTA GTTTTATTTT TCCAGATTGG 18360 .......... .......... .......... .......... .......... .......... 134 TTTTGAAAAA AATAATTTGA ATTAATACTT TTGCTTTGCG TGTGGATTTA CAAGTTAGGA 18300 .......... .......... .......... .......... .......... .......... 134 GGAAATTGAT TTTACAATGG ACTTGAACTC CAATTGAAGA ACAATTGATG GATCTATCAT 18240 .......... .......... .......... .......... .......... .......... 134 GCTATTCTCA GAAATTTTAG TGTAGTTTTT TATTTTTAAG GCTTTGTTTT TGTGGATTTG 18180 .......... .......... .......... .......... .......... .......... 134 TAGAAGATCT TCAGCTAGAT CAAACTGAGA AATTTTAAAG AATTCCTACA ATTTTTCAGT 18120 .......... .......... .......... .......... .......... .......... 134 AGTTAAATTG TATAACATTG ACCGAGATGA CTGATATCTT TTGATTTATG GATTAAAGTT 18060 .......... .......... .......... .......... .......... .......... 134 CTTTAAATCG TCTATCATAT ACTGTATCTA CCTTTGAATA TATTGGTGCT AAACTTTGAA 18000 .......... .......... .......... .......... .......... .......... 134 ATTGAATTGT ATACATGACT TAATTTGAAG CAACAACGTA TGAGTAGATG CTTTTTTTTT 17940 .......... .......... .......... .......... .......... .......... 134 TGCATTCGTA GAACTCTCCA TGCAAATGGT ATGTGCTATC TTTCCCTTTT ACACCTTAAA 17880 .......... .......... .......... .......... .......... .......... 134 GCTTAAGTAA GTAAATCAAT GAATGTTTGA TGCAGGCTCG TCCCTGATTC AAGAGTACAG 17820 ||||| |||||||||| |||||||||| .......... .......... .......... .....GCTCG TCCCTGATTC AAGAGTACAG 159 TGACAGTTGC TCCTTATTTA TATAAAGCTC TAGAATTGTG GTGGAAGACA ATGCAGGTGA 17760 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| TGACAGTTGC TCCTTATTTA TATAAAGCTC TAGAATTGTG GTGGAAGACA ATGCAG.... 215 ATATCACTAT TCAAAATATA GACTTATGTC CTACATATGA TGTCATTTTT TTGGGTTGAT 17700 .......... .......... .......... .......... .......... .......... 215 ACAAGCCAGT TAAGAACACA CTACAAACTC ACAGCAAAAT ATGGGAACAC AAAAATCATA 17640 .......... .......... .......... .......... .......... .......... 215 TGGTCTTGGC AGTTTGTTGT GGGATAGAGC CTTAGATAAG ATCTCATCAA ATGTTAGATG 17580 .......... .......... .......... .......... .......... .......... 215 TGAACATAAA TATATCTTTG TTATCAAATT TGTAACACTT TTTTGTCATT GCTTCTCTCC 17520 .......... .......... .......... .......... .......... .......... 215 ACACAATTTC TTTCTGCTTT GAATTAGAAT AATATGATTG TATCCATTTT TGTTTATTGA 17460 .......... .......... .......... .......... .......... .......... 215 CCCTTGGTTG ATCAAATAAG TCAAACAGTC TCTTGTGCCT GCTGCATTTG AAGATACATA 17400 .......... .......... .......... .......... .......... .......... 215 TTTGTGTTTC AGCTCGTTCA CAGGCTTGAA ACTCTTTATT TCTAGTCTAC TTCAGTAGAT 17340 .......... .......... .......... .......... .......... .......... 215 TGCTAGTGGA TGCTGGTGGT TCTCTGATAT TTAAGACCCT CTTTCATGGA AGAGTTTGAA 17280 .......... .......... .......... .......... .......... .......... 215 ATGTATAAAG ATGATCTCTG TAAATAAAAG TGTGTCCTTG TCTGCTAGAG GTAGTCATGG 17220 .......... .......... .......... .......... .......... .......... 215 GAAAATCTAC AAAAGAAATA AACAAAGACA GCACAATGAG AAAATATAAT GACCACAGCT 17160 .......... .......... .......... .......... .......... .......... 215 GAGGAAAAGA AGAAAACTAA GTGTGATCGA CTTTTTCAAT TGTAGTTTAT TACATTATTT 17100 .......... .......... .......... .......... .......... .......... 215 TGTTCTTTGG TACGATTGAC TTTCATAATA TAAGTTAACA TAAACTCCTA ATCTTCCAAC 17040 .......... .......... .......... .......... .......... .......... 215 TTGTTCTCTA CTTCTTCTCT TTGCTATTTC AGTTTATTCA TAAGTGAATG TTATAATTGA 16980 .......... .......... .......... .......... .......... .......... 215 AATAATATTA TGACTTAAAG ATTGCATCAA TAGTGTTGGT TATGCTCACT TCTGTTTGTT 16920 .......... .......... .......... .......... .......... .......... 215 GAAGTTACTT TCTGCTTAGA AATTAGTAGC TTAAACAGGC ATAACTTGCG TGTATTTAAC 16860 .......... .......... .......... .......... .......... .......... 215 TGACTAAGAA GGCTCCAAGT TCAAGTCACT ATACCAGTTA ATAATAGTTG CTTGATTTGT 16800 .......... .......... .......... .......... .......... .......... 215 GATCTTCCTA AATCTCATTA GTTCACCTGA TGGCATGTCA GAGTCCAATC AGGCACTAGT 16740 .......... .......... .......... .......... .......... .......... 215 TATCTTCTTA CACTGGTACC TGGACACATA TATTTGCCGA AACTTCAACG TTCTAAACTT 16680 .......... .......... .......... .......... .......... .......... 215 TCTGGGGAAT AATGTCATTA AGGCCCTAAA GCTTTGTCTA TGTAATTTGT GTGTCTTTTT 16620 .......... .......... .......... .......... .......... .......... 215 TTTTTTTTTT TTTAATAATT TTGTTTTCTT GTATTGCATA TACATCCTTT AATTTTTATC 16560 .......... .......... .......... .......... .......... .......... 215 TTCATCAGGC CCTTTACTAG AACTTTTTTT GGATGTGTGT TGTGCTTAAA GCCCAACCTT 16500 .......... .......... .......... .......... .......... .......... 215 TGAATCTTCC TCTGCCTTTG ACAACACTGC TGAAGAATGA TGTTTGTGCT GTTTCATCAA 16440 .......... .......... .......... .......... .......... .......... 215 GTTCTTGGAT CCTGCATATT GAGACTTATT TGAAAGTTCC CTACTCTTTG GTCCATGTTG 16380 .......... .......... .......... .......... .......... .......... 215 AGCATCTGTT TTTGCGGAGT CTTTTTTTAA AAACTCTTTT CTGGGGGTCA TTCTTACCTT 16320 .......... .......... .......... .......... .......... .......... 215 TGACTATGAA AAGTTAAAGA TGATGCAATG TTGTCTCTGC AGTTGTAGCT CTGACAATTG 16260 |||||||| |||||||||| .......... .......... .......... .......... ..TTGTAGCT CTGACAATTG 233 GCATATCACC ACCTGGGTCA ATGAGGAATG CAACTATGGC AACTGGATCT TCTAATCTTA 16200 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATATCACC ACCTGGGTCA ATGAGGAATG CAACTATGGC AACTGGATCT TCTAATCTTA 293 AAGTGCCTAC TATGCGAATC ACAAGAGCAC GGGCAAAAAC CTTGGGTTCA TCAGGAGGAC 16140 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGCCTAC TATGCGAATC ACAAGAGCAC GGGCAAAAAC CTTGGGTTCA TCAGGAGGAC 353 TACCACCTCT ACACCCATCT GTCAGACAGG ATAAGAAACA GGGACTTGTA ACACAAGGAA 16080 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACCACCTCT ACACCCATCT GTCAGACAGG ATAAGAAACA GGGACTTGTA ACACAAGGAA 413 CAAAATCCAA AAGACCAGCC CCAGATGAGA ACAAACCAGC TAATTCTTCC AGTACTGCTT 16020 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAAATCCAA AAGACCAGCC CCAGATGAGA ACAAACCAGC TAATTCTTCC AGTACTGCTT 473 CTCAACAGCC TAAGCGAAGG GCTGTTCTCA GGGATGTCAC TAATGTGCTT TGTGAAAATC 15960 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAACAGCC TAAGCGAAGG GCTGTTCTCA GGGATGTCAC TAATGTGCTT TGTGAAAATC 533 CGTACATGAA TTGCATCAAT GGAAGCAAAT TTCAGGTGAG CTTCAATGGA TGTGTTATAT 15900 |||||||||| |||||||||| |||||||||| ||||| CGTACATGAA TTGCATCAAT GGAAGCAAAT TTCAG..... .......... .......... 568 CATATTTGCT CTTTGGAAAT GAAATCATTC TGCTAAACAT AATTATTGGT TATTGAACTA 15840 .......... .......... .......... .......... .......... .......... 568 ATGTCTTTGT TTCAAAAGAT GGAGATTCAG TGCTAAATCT TTTTCTTTGT TTCAAAAACT 15780 .......... .......... .......... .......... .......... .......... 568 TATCAACTTC TGGCCAATGA TGTCAACTTG TCCTATGGTG ATAGGTTAAG AAATTCTCTG 15720 |||||| |||||||||| .......... .......... .......... .......... ....GTTAAG AAATTCTCTG 584 ATAAGAGGAA TTCAAAGGTG ACACCTGCTC TTTTGGTGAA AAAGCCTGAG CTTGAAGATA 15660 |||||||||| |||||||||| |||||||||| |||||||||| |||| ||||| |||||||||| ATAAGAGGAA TTCAAAGGTG ACACCTGCTC TTTTGGTGAA AAAG-CTGAG CTTGAAGATA 643 GAAAAGAGAG TGTGATTGAA GAAGCAAAAA TG 15628 |||||||||| |||||||||| |||||||||| || GAAAAGAGAG TGTGATTGAA GAAGCAAAAA TG 675 hqPGS_C06HBa0046G10.1-5-_SGN-E314602+ (19139 19005,17844 17764,16277 15925,15735 15628) Total number of EST alignments reported: 8 ________________________________________________________________________________ Predicted gene locations (5) in segment 1 to 19191: PGL 1 (- strand): 2549 1927 AGS-1 (2549 1927) SCR (e 0.997) Exon 1 2549 1927 ( 623 n); score: 0.997 PGS (2549 1927) SGN-E342700- 3-phase translation of AGS-1 (-strand): . . . . . . 2549 AACCCTAGAGCTGTAATACACAACCAAGATCTAAACCAAAACCCCATTGGCTAAAAAATC N P R A V I H N Q D L N Q N P I G - K I T L E L - Y T T K I - T K T P L A K K S P - S C N T Q P R S K P K P H W L K N . . . . . . 2489 TGCAAACTTTAAGCTAAACCACAAAATGGGGGTGCCATTATCTTCAGCAAGAAGAAAGAA C K L - A K P Q N G G A I I F S K K K E A N F K L N H K M G V P L S S A R R K K L Q T L S - T T K W G C H Y L Q Q E E R . . . . . . 2429 AAATCAAATCTTTCCTCAAACTAACTTAAAGGGTGGTTCAGATCTTGATTTACAAATAGG K S N L S S N - L K G W F R S - F T N R N Q I F P Q T N L K G G S D L D L Q I G K I K S F L K L T - R V V Q I L I Y K - . . . . . . 2369 GAAAAGATTGAAAAAAAATGCAAGAATCATGCAAAGCTGAAGCCTTTTTTGCTCAATTTA E K I E K K C K N H A K L K P F L L N L K R L K K N A R I M Q S - S L F C S I Y G K D - K K M Q E S C K A E A F F A Q F . . . . . . 2309 CAACCTTGGATTCTTTAAATAAAAAGAGAGAGAGCCCAGATCTCATACACATTCATTATA Q P W I L - I K R E R A Q I S Y T F I I N L G F F K - K E R E P R S H T H S L - T T L D S L N K K R E S P D L I H I H Y . . . . . . 2249 ACTATTTATATATACATAACATGAAAAAGATTCAGATATATTGCAGAGAAGGGGGTAGGG T I Y I Y I T - K R F R Y I A E K G V G L F I Y T - H E K D S D I L Q R R G - G N Y L Y I H N M K K I Q I Y C R E G G R . . . . . . 2189 AGGGTGTCAGGATGTGGGTAGGTTGGGTGGGTGGTGGGTTGAAGAGGGGAGAAAGAAGGA R V S G C G - V G W V V G - R G E K E G G C Q D V G R L G G W W V E E G R K K E E G V R M W V G W V G G G L K R G E R R . . . . . . 2129 GATTCAGATGCTGATAACAAGGCTAAAGAAAACAAAAAATGTCATTATTCATCATTCAAT D S D A D N K A K E N K K C H Y S S F N I Q M L I T R L K K T K N V I I H H S I R F R C - - Q G - R K Q K M S L F I I Q . . . . . . 2069 TTCTTCATGCACTAGTACTATGTACATACATTTTATACATAAACATAAAAACAACAAGTG F F M H - Y Y V H T F Y T - T - K Q Q V S S C T S T M Y I H F I H K H K N N K - F L H A L V L C T Y I L Y I N I K T T S . . . . . . 2009 AATAAAGCTTTCTTCTTCATTAGGAAATTTTTGGGTTTGAAGGGGGTGGGGTAAGTGAGC N K A F F F I R K F L G L K G V G - V S I K L S S S L G N F W V - R G W G K - A E - S F L L H - E I F G F E G G G V S E . . . 1949 TTAATTATGAACCTTGGCATGGA L I M N L G M - L - T L A W L N Y E P W H G Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-5-_PGL-1_AGS-1_PPS_1 (2358 2116) (frame '0'; 240 bp, 80 residues) 1 KKMQESCKAE AFFAQFTTLD SLNKKRESPD LIHIHYNYLY IHNMKKIQIY CREGGREGVR 61 MWVGWVGGGL KRGERRRFRC - 3-phase translation of AGS-1 (+strand): . . . . . . 1927 TCCATGCCAAGGTTCATAATTAAGCTCACTTACCCCACCCCCTTCAAACCCAAAAATTTC S M P R F I I K L T Y P T P F K P K N F P C Q G S - L S S L T P P P S N P K I S H A K V H N - A H L P H P L Q T Q K F . . . . . . 1987 CTAATGAAGAAGAAAGCTTTATTCACTTGTTGTTTTTATGTTTATGTATAAAATGTATGT L M K K K A L F T C C F Y V Y V - N V C - - R R K L Y S L V V F M F M Y K M Y V P N E E E S F I H L L F L C L C I K C M . . . . . . 2047 ACATAGTACTAGTGCATGAAGAAATTGAATGATGAATAATGACATTTTTTGTTTTCTTTA T - Y - C M K K L N D E - - H F L F S L H S T S A - R N - M M N N D I F C F L - Y I V L V H E E I E - - I M T F F V F F . . . . . . 2107 GCCTTGTTATCAGCATCTGAATCTCCTTCTTTCTCCCCTCTTCAACCCACCACCCACCCA A L L S A S E S P S F S P L Q P T T H P P C Y Q H L N L L L S P L F N P P P T Q S L V I S I - I S F F L P S S T H H P P . . . . . . 2167 ACCTACCCACATCCTGACACCCTCCCTACCCCCTTCTCTGCAATATATCTGAATCTTTTT T Y P H P D T L P T P F S A I Y L N L F P T H I L T P S L P P S L Q Y I - I F F N L P T S - H P P Y P L L C N I S E S F . . . . . . 2227 CATGTTATGTATATATAAATAGTTATAATGAATGTGTATGAGATCTGGGCTCTCTCTCTT H V M Y I - I V I M N V Y E I W A L S L M L C I Y K - L - - M C M R S G L S L F S C Y V Y I N S Y N E C V - D L G S L S . . . . . . 2287 TTTATTTAAAGAATCCAAGGTTGTAAATTGAGCAAAAAAGGCTTCAGCTTTGCATGATTC F I - R I Q G C K L S K K G F S F A - F L F K E S K V V N - A K K A S A L H D S F Y L K N P R L - I E Q K R L Q L C M I . . . . . . 2347 TTGCATTTTTTTTCAATCTTTTCCCTATTTGTAAATCAAGATCTGAACCACCCTTTAAGT L H F F S I F S L F V N Q D L N H P L S C I F F Q S F P Y L - I K I - T T L - V L A F F F N L F P I C K S R S E P P F K . . . . . . 2407 TAGTTTGAGGAAAGATTTGATTTTTCTTTCTTCTTGCTGAAGATAATGGCACCCCCATTT - F E E R F D F S F F L L K I M A P P F S L R K D L I F L S S C - R - W H P H F L V - G K I - F F F L L A E D N G T P I . . . . . . 2467 TGTGGTTTAGCTTAAAGTTTGCAGATTTTTTAGCCAATGGGGTTTTGGTTTAGATCTTGG C G L A - S L Q I F - P M G F W F R S W V V - L K V C R F F S Q W G F G L D L G L W F S L K F A D F L A N G V L V - I L . . . 2527 TTGTGTATTACAGCTCTAGGGTT L C I T A L G C V L Q L - G V V Y Y S S R V Maximal non-overlapping open reading frames (>= 64 codons): none PGL 2 (+ strand): 3648 5829 AGS-1 (3648 3783,5282 5829) SCR (e 1.000 d 0.998 a 0.795,e 0.996) Exon 1 3648 3783 ( 136 n); score: 1.000 Intron 1 3784 5281 (1498 n); Pd: 0.998 Pa: 0.795 Exon 2 5282 5829 ( 548 n); score: 0.996 PGS (3648 3783,5282 5829) SGN-E318334+ PGS (3648 3783,5282 5828) SGN-E318159+ 3-phase translation of AGS-1 (+strand): . . . . . . 3648 AAGCACCCAAAAGGAGGTGATGTTTTTGAGTGAGCTGGAAGAAATTTTGGAAGTTATTAA K H P K G G D V F E - A G R N F G S Y - S T Q K E V M F L S E L E E I L E V I N A P K R R - C F - V S W K K F W K L L . . . . . . 3708 CATGGCTGAGTTCCAAAAAGTGATGGTTCCCTTGTTCTGGCGGATCGGTTGCTGCATCAA H G - V P K S D G S L V L A D R L L H Q M A E F Q K V M V P L F W R I G C C I N T W L S S K K - W F P C S G G S V A A S . . : . . . . 3768 TAGTTACCATTTTCAG : GTTGCTGAAAGGGCTCTCTTCCTTTGGAATAATGACCAAATTGT - L P F S : G C - K G S L P L E - - P N C S Y H F Q : V A E R A L F L W N N D Q I V I V T I F R : L L K G L S S F G I M T K L . . . . . . 5326 CAATTTGATTGCACATAACCGGCACGTGATTCTCCCGATTATATTCCCAGCTGTGGAAAG Q F D C T - P A R D S P D Y I P S C G K N L I A H N R H V I L P I I F P A V E S S I - L H I T G T - F S R L Y S Q L W K . . . . . . 5386 CAATACTCAGAACCACTGGAATCATGCTGTGCAGAACTTGAGTCTAAATGTAAGAAGGAT Q Y S E P L E S C C A E L E S K C K K D N T Q N H W N H A V Q N L S L N V R R M A I L R T T G I M L C R T - V - M - E G . . . . . . 5446 GTTCTCCGAAATGGATGATGTACTTTTCCTTGCTTGTCATTCTCATTACAAGGAAGAACA V L R N G - C T F P C L S F S L Q G R T F S E M D D V L F L A C H S H Y K E E Q C S P K W M M Y F S L L V I L I T R K N . . . . . . 5506 GGAGAAAATAGACTTGGAATCTGAAAAACGGAAGGAAGCATGGGAACAGCTGGAGACTGC G E N R L G I - K T E G S M G T A G D C E K I D L E S E K R K E A W E Q L E T A R R K - T W N L K N G R K H G N S W R L . . . . . . 5566 AGCTAGTCTACGTCCACCTGTAGCTGGAAACATTGCTGTCCTGGTAACGCCTTTAGCAAC S - S T S T C S W K H C C P G N A F S N A S L R P P V A G N I A V L V T P L A T Q L V Y V H L - L E T L L S W - R L - Q . . . . . . 5626 TTCAATCACTTGCTAAGAGGTGATGACTCCACTCTATCCTTGTCCCCGTGTTTTTCTGTG F N H L L R G D D S T L S L S P C F S V S I T C - E V M T P L Y P C P R V F L C L Q S L A K R - - L H S I L V P V F F C . . . . . . 5686 TATGTTCCACGCAATCTAGGAATCATGGATGGAAAATGGCTGTTGTCTGGAGCAGGTTTT Y V P R N L G I M D G K W L L S G A G F M F H A I - E S W M E N G C C L E Q V F V C S T Q S R N H G W K M A V V W S R F . . . . . . 5746 TGAAGATCAACCCGATTAACAGCCTTATCTCTGTTGTAACCTTAGTCCTGCAACTGTGTT - R S T R L T A L S L L - P - S C N C V E D Q P D - Q P Y L C C N L S P A T V L L K I N P I N S L I S V V T L V L Q L C . . . 5806 GAGAGCGCGAATGGGGGGGGGGGG E S A N G G G G R A R M G G G - E R E W G G G Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-5+_PGL-2_AGS-1_PPS_1 (3649 3783,5282 5641) (frame '2'; 492 bp, 164 residues) 1 STQKEVMFLS ELEEILEVIN MAEFQKVMVP LFWRIGCCIN SYHFQVAERA LFLWNNDQIV 61 NLIAHNRHVI LPIIFPAVES NTQNHWNHAV QNLSLNVRRM FSEMDDVLFL ACHSHYKEEQ 121 EKIDLESEKR KEAWEQLETA ASLRPPVAGN IAVLVTPLAT SITC- PGL 3 (+ strand): 7900 8302 AGS-1 (7900 8302) SCR (e 0.885) Exon 1 7900 8302 ( 403 n); score: 0.885 PGS (7900 8302) SGN-E231056+ PGS (7900 8141) SGN-E240778+ 3-phase translation of AGS-1 (+strand): . . . . . . 7900 AAAATATTACACAAGATGAGCCAATTGATCCTGTGAATATCAATAATCGTGATCTTCCTA K I L H K M S Q L I L - I S I I V I F L K Y Y T R - A N - S C E Y Q - S - S S - N I T Q D E P I D P V N I N N R D L P . . . . . . 7960 ATTACGGCTCACCTTCAGCTAGTAATAGTGATGATTTGCCTAATGCTGAGGAATCAGGAG I T A H L Q L V I V M I C L M L R N Q E L R L T F S - - - - - F A - C - G I R R N Y G S P S A S N S D D L P N A E E S G . . . . . . 8020 ATAATGTTCCATTTGAGGCATCATCTAGTGATGATGATTTTGACTCCCATTCGATCGCCA I M F H L R H H L V M M I L T P I R S P - C S I - G I I - - - - F - L P F D R Q D N V P F E A S S S D D D F D S H S I A . . . . . . 8080 AGACCAATGTCCATGAGTCCGTTTCGTAATCATTAATTCCTTATCTTGATCATCTCCCGG R P M S M S P F R N H - F L I L I I S R D Q C P - V R F V I I N S L S - S S P G K T N V H E S V S - S L I P Y L D H L P . . . . . . 8140 ATAGTCCATATATCTTTGGTGATACACATGATGAGTATACATCACAACGTACGTGGCATG I V H I S L V I H M M S I H H N V R G M - S I Y L W - Y T - - V Y I T T Y V A - D S P Y I F G D T H D E Y T S Q R T W H . . . . . . 8200 AACCAGAAGATTTCATGAATGATGCTATTTATATTGAAAAAGACATGTTATTCAATTCAA N Q K I S - M M L F I L K K T C Y S I Q T R R F H E - C Y L Y - K R H V I Q F K E P E D F M N D A I Y I E K D M L F N S . . . . . 8260 ATAAGCAGTTGCAAAGAGCAGTAAAACTTTTACATTTGAAGAT I S S C K E Q - N F Y I - R - A V A K S S K T F T F E D N K Q L Q R A V K L L H L K Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-5+_PGL-3_AGS-1_PPS_1 (7902 8108) (frame '0'; 204 bp, 68 residues) 1 NITQDEPIDP VNINNRDLPN YGSPSASNSD DLPNAEESGD NVPFEASSSD DDFDSHSIAK 61 TNVHESVS- >C06HBa0046G10.1-5+_PGL-3_AGS-1_PPS_2 (8109 8300) (frame '0'; 192 bp, 64 residues) 1 SLIPYLDHLP DSPYIFGDTH DEYTSQRTWH EPEDFMNDAI YIEKDMLFNS NKQLQRAVKL 61 LHLK 3-phase translation of AGS-1 (-strand): . . . . . . 8302 ATCTTCAAATGTAAAAGTTTTACTGCTCTTTGCAACTGCTTATTTGAATTGAATAACATG I F K C K S F T A L C N C L F E L N N M S S N V K V L L L F A T A Y L N - I T C L Q M - K F Y C S L Q L L I - I E - H . . . . . . 8242 TCTTTTTCAATATAAATAGCATCATTCATGAAATCTTCTGGTTCATGCCACGTACGTTGT S F S I - I A S F M K S S G S C H V R C L F Q Y K - H H S - N L L V H A T Y V V V F F N I N S I I H E I F W F M P R T L . . . . . . 8182 GATGTATACTCATCATGTGTATCACCAAAGATATATGGACTATCCGGGAGATGATCAAGA D V Y S S C V S P K I Y G L S G R - S R M Y T H H V Y H Q R Y M D Y P G D D Q D - C I L I M C I T K D I W T I R E M I K . . . . . . 8122 TAAGGAATTAATGATTACGAAACGGACTCATGGACATTGGTCTTGGCGATCGAATGGGAG - G I N D Y E T D S W T L V L A I E W E K E L M I T K R T H G H W S W R S N G S I R N - - L R N G L M D I G L G D R M G . . . . . . 8062 TCAAAATCATCATCACTAGATGATGCCTCAAATGGAACATTATCTCCTGATTCCTCAGCA S K S S S L D D A S N G T L S P D S S A Q N H H H - M M P Q M E H Y L L I P Q H V K I I I T R - C L K W N I I S - F L S . . . . . . 8002 TTAGGCAAATCATCACTATTACTAGCTGAAGGTGAGCCGTAATTAGGAAGATCACGATTA L G K S S L L L A E G E P - L G R S R L - A N H H Y Y - L K V S R N - E D H D Y I R Q I I T I T S - R - A V I R K I T I . . . . . 7942 TTGATATTCACAGGATCAATTGGCTCATCTTGTGTAATATTTT L I F T G S I G S S C V I F - Y S Q D Q L A H L V - Y F I D I H R I N W L I L C N I Maximal non-overlapping open reading frames (>= 64 codons): none PGL 4 (- strand): 12891 11190 AGS-1 (11895 11657,11569 11536,11453 11304,11218 11190) SCR (e 1.000 d 0.967 a 0.893,e 1.000 d 0.785 a 0.965,e 1.000 d 0.997 a 0.949,e 1.000) Exon 1 11895 11657 ( 239 n); score: 1.000 Intron 1 11656 11570 ( 87 n); Pd: 0.967 Pa: 0.893 Exon 2 11569 11536 ( 34 n); score: 1.000 Intron 2 11535 11454 ( 82 n); Pd: 0.785 Pa: 0.965 Exon 3 11453 11304 ( 150 n); score: 1.000 Intron 3 11303 11219 ( 85 n); Pd: 0.997 Pa: 0.949 Exon 4 11218 11190 ( 29 n); score: 1.000 PGS (11895 11657,11569 11536,11453 11304,11218 11190) SGN-E313671+ 3-phase translation of AGS-1 (-strand): . . . . . . 11895 TGAGCTTTCCTTTCTCTTTTATGATATCTTGGATGCATACTTTTTTCCTACTTGAGTAAT - A F L S L L - Y L G C I L F S Y L S N E L S F L F Y D I L D A Y F F P T - V M S F P F S F M I S W M H T F F L L E - . . . . . . 11835 GATCTGAACATTTCCCAAATGTTTTCCTTTGTCTTCTATATACGACTTGAAGTTCCCTGT D L N I S Q M F S F V F Y I R L E V P C I - T F P K C F P L S S I Y D L K F P V - S E H F P N V F L C L L Y T T - S S L . . . . . . 11775 GGATGTGAAAAGGCTAATTACTAATTGCATATCCATTGTCAGGTAGTAAGAATGGAAAGT G C E K A N Y - L H I H C Q V V R M E S D V K R L I T N C I S I V R - - E W K V W M - K G - L L I A Y P L S G S K N G K . . . . . . : 11715 CTGGTTCTGAATTTTTTGGGCTTTCAACTTGCTGCTCCGACCACCAAAAAGTTCCTGAG : G L V L N F L G F Q L A A P T T K K F L R : W F - I F W A F N L L L R P P K S S - : G S G S E F F G L S T C C S D H Q K V P E : . . . . : . . 11568 AGATTCGTTCAAGCATCACAAGCTTCTTATGAG : GTTCCCTCTGTTGAACTGGAATTCATG R F V Q A S Q A S Y E : V P S V E L E F M D S F K H H K L L M R : F P L L N W N S W E I R S S I T S F L - : G S L C - T G I H . . . . . . 11426 GCAAACTATTTAGCAGAGTTAACACTAGCTGAATATAGTTTTCTCAAGTTTCTTCCATCT A N Y L A E L T L A E Y S F L K F L P S Q T I - Q S - H - L N I V F S S F F H L G K L F S R V N T S - I - F S Q V S S I . . . . . . 11366 GTTACTGCTGCATCAGCTGTATTTCTAGCCAGATGGACACTTGATCAATCTAACCACCCA V T A A S A V F L A R W T L D Q S N H P L L L H Q L Y F - P D G H L I N L T T H C Y C C I S C I S S Q M D T - S I - P P . : . . . 11306 TGG : AATTCAACTTTGGAGCACTACACTACATA W : N S T L E H Y T T G : I Q L W S T T L H M : E F N F G A L H Y I Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-5-_PGL-4_AGS-1_PPS_1 (11751 11657,11569 11536,11453 11304,11218 11192) (frame '1'; 306 bp, 102 residues) 1 LHIHCQVVRM ESLVLNFLGF QLAAPTTKKF LRRFVQASQA SYEVPSVELE FMANYLAELT 61 LAEYSFLKFL PSVTAASAVF LARWTLDQSN HPWNSTLEHY TT AGS-2 (12891 12860,12534 12459,11733 11666) SCR (e 1.000 d 0.922 a 0.997,e 1.000 d 0.896 a 0.710,e 0.949) Exon 1 12891 12860 ( 32 n); score: 1.000 Intron 1 12859 12535 ( 325 n); Pd: 0.922 Pa: 0.997 Exon 2 12534 12459 ( 76 n); score: 1.000 Intron 2 12458 11734 ( 725 n); Pd: 0.896 Pa: 0.710 Exon 3 11733 11666 ( 68 n); score: 0.949 PGS (12891 12860,12534 12459,11733 11666) SGN-E237597+ 3-phase translation of AGS-2 (-strand): . . . . : . . 12891 CTGCTTGGAGTTACCTGCATGCTAATTGCTTC : CAAATATGAAGAAATTTGTGCACCTCGT L L G V T C M L I A S : K Y E E I C A P R C L E L P A C - L L : P N M K K F V H L V A W S Y L H A N C F : Q I - R N L C T S . . . . . : . 12506 GTGGAAGAATTTTGCTTTATTACAGACAACACTTACTCGAAAGAAGAG : GTAGTAAGAATG V E E F C F I T D N T Y S K E E : V V R M W K N F A L L Q T T L T R K K R : - - E W C G R I L L Y Y R Q H L L E R R : G S K N . . . . . . 11721 GAAAGTCTGGTTCTGAATTTTTTGGGCTTTCAACTTGCTGCTCCGACCACCAAAAA E S L V L N F L G F Q L A A P T T K K V W F - I F W A F N L L L R P P K G K S G S E F F G L S T C C S D H Q K Maximal non-overlapping open reading frames (>= 64 codons): none PGL 5 (- strand): 19139 15628 AGS-1 (19139 19005,17844 17764,16277 15925,15735 15628) SCR (e 0.985 d 0.969 a 0.958,e 1.000 d 0.927 a 0.999,e 1.000 d 0.988 a 0.970,e 0.991) Exon 1 19139 19005 ( 135 n); score: 0.985 Intron 1 19004 17845 (1160 n); Pd: 0.969 Pa: 0.958 Exon 2 17844 17764 ( 81 n); score: 1.000 Intron 2 17763 16278 (1486 n); Pd: 0.927 Pa: 0.999 Exon 3 16277 15925 ( 353 n); score: 1.000 Intron 3 15924 15736 ( 189 n); Pd: 0.988 Pa: 0.970 Exon 4 15735 15628 ( 108 n); score: 0.991 PGS (19139 19005,17844 17764,16277 15925,15735 15628) SGN-E314602+ 3-phase translation of AGS-1 (-strand): . . . . . . 19139 ACCCCTTTTGGAACCACACAGACGAGCTACATTGATTGTATGACCGATCGGAGAAGGTAG T P F G T T Q T S Y I D C M T D R R R - P L L E P H R R A T L I V - P I G E G R P F W N H T D E L H - L Y D R S E K V . . . . . . 19079 AAACCCTAGCTCTGCAATCGAGGCACCGCCGTAGAACGGTGTTGCGGGCAGCTCATGAGC K P - L C N R G T A V E R C C G Q L M S N P S S A I E A P P - N G V A G S S - A E T L A L Q S R H R R R T V L R A A H E . . : . . . . 19019 TGTTAATAATGCTTA : GCTCGTCCCTGATTCAAGAGTACAGTGACAGTTGCTCCTTATTTA C - - C L : A R P - F K S T V T V A P Y L V N N A - : L V P D S R V Q - Q L L L I Y L L I M L : S S S L I Q E Y S D S C S L F . . . . : . . 17799 TATAAAGCTCTAGAATTGTGGTGGAAGACAATGCAG : TTGTAGCTCTGACAATTGGCATAT Y K A L E L W W K T M Q : L - L - Q L A Y I K L - N C G G R Q C S : C S S D N W H I I - S S R I V V E D N A : V V A L T I G I . . . . . . 16253 CACCACCTGGGTCAATGAGGAATGCAACTATGGCAACTGGATCTTCTAATCTTAAAGTGC H H L G Q - G M Q L W Q L D L L I L K C T T W V N E E C N Y G N W I F - S - S A S P P G S M R N A T M A T G S S N L K V . . . . . . 16193 CTACTATGCGAATCACAAGAGCACGGGCAAAAACCTTGGGTTCATCAGGAGGACTACCAC L L C E S Q E H G Q K P W V H Q E D Y H Y Y A N H K S T G K N L G F I R R T T T P T M R I T R A R A K T L G S S G G L P . . . . . . 16133 CTCTACACCCATCTGTCAGACAGGATAAGAAACAGGGACTTGTAACACAAGGAACAAAAT L Y T H L S D R I R N R D L - H K E Q N S T P I C Q T G - E T G T C N T R N K I P L H P S V R Q D K K Q G L V T Q G T K . . . . . . 16073 CCAAAAGACCAGCCCCAGATGAGAACAAACCAGCTAATTCTTCCAGTACTGCTTCTCAAC P K D Q P Q M R T N Q L I L P V L L L N Q K T S P R - E Q T S - F F Q Y C F S T S K R P A P D E N K P A N S S S T A S Q . . . . . . 16013 AGCCTAAGCGAAGGGCTGTTCTCAGGGATGTCACTAATGTGCTTTGTGAAAATCCGTACA S L S E G L F S G M S L M C F V K I R T A - A K G C S Q G C H - C A L - K S V H Q P K R R A V L R D V T N V L C E N P Y . . . : . . . 15953 TGAATTGCATCAATGGAAGCAAATTTCAG : GTTAAGAAATTCTCTGATAAGAGGAATTCAA - I A S M E A N F R : L R N S L I R G I Q E L H Q W K Q I S : G - E I L - - E E F K M N C I N G S K F Q : V K K F S D K R N S . . . . . . 15704 AGGTGACACCTGCTCTTTTGGTGAAAAAGCCTGAGCTTGAAGATAGAAAAGAGAGTGTGA R - H L L F W - K S L S L K I E K R V - G D T C S F G E K A - A - R - K R E C D K V T P A L L V K K P E L E D R K E S V . . 15644 TTGAAGAAGCAAAAATG L K K Q K - R S K N I E E A K M Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-5-_PGL-5_AGS-1_PPS_1 (17794 17764,16277 15925,15735 15628) (frame '0'; 492 bp, 164 residues) 1 SSRIVVEDNA VVALTIGISP PGSMRNATMA TGSSNLKVPT MRITRARAKT LGSSGGLPPL 61 HPSVRQDKKQ GLVTQGTKSK RPAPDENKPA NSSSTASQQP KRRAVLRDVT NVLCENPYMN 121 CINGSKFQVK KFSDKRNSKV TPALLVKKPE LEDRKESVIE EAKM ... finished at: Mon Aug 28 21:42:17 2006 ________________________________________________________________________________ Sequence 6: C06HBa0046G10.1-6, from 1 to 4924, both strands analyzed. ... started at: Mon Aug 28 21:42:17 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 5 ... matches indexed, elapsed seconds = 5 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 0 No significant EST matches were found. ... finished at: Mon Aug 28 21:42:25 2006 ________________________________________________________________________________ Sequence 7: C06HBa0046G10.1-7, from 1 to 1885, both strands analyzed. ... started at: Mon Aug 28 21:42:25 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 3 ... matches indexed, elapsed seconds = 4 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 3 ... matches indexed, elapsed seconds = 3 HitsTableSize = 0 No significant EST matches were found. ... finished at: Mon Aug 28 21:42:33 2006 ________________________________________________________________________________ Sequence 8: C06HBa0046G10.1-8, from 1 to 5464, both strands analyzed. ... started at: Mon Aug 28 21:42:33 2006 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 4 ... matches indexed, elapsed seconds = 4 HitsTableSize = 0 EST library file: /tmp/cxgn-bacpublish-resources-P1gOWF/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 3 ... matches indexed, elapsed seconds = 3 HitsTableSize = 2 ******************************************************************************** EST sequence 2 -strand 624 n (File: SGN-E542827-) 1 CCAAAACCAG CAACCTAAAG TTCAAAATTC CAGGAAATTT CAATGTTTTC ATCCAATATA 61 TGGTAGAAAT TTCTAACAAT TTTATAACTT TTCGATGATT TGGTTCACTG CTAGTGTTTG 121 AACTTTGTAT TTGATTGAAG ATTTTGTGTT GTTTACAAAT ATCCTCTTTT AAGTTTATCT 181 ATTTTACGGA AAAAGTTTGA TATATTTCTC AATTTTGTCA TTTGCAGCAC TGTCGTTACA 241 AAATGGCTAA TATATATTAA AAAATTAATT TTAAATTTAT ATTTATTACT TTTGATTGTT 301 TTTAAAAAAA TTATTTAAAG GTATATATTA TTCTTCTATC AAAGTTCGAG GTTATATTTA 361 ATTTTTTTTA TACATAAATT ATTGTTTGAC TTCTTTTATT ATAATTATTT GAGTTTGTTA 421 TTCTAATTTT TTTTCTTTCA TTCCTTAGTT TAAAGAGAAA AAAAACTAAA CTATTTTTTT 481 TGTGTGTATT GTAATTTAAT TTGGTATTCA AAGAAAAATT TTGGTCATCT ACAATAAGTT 541 TTACAAGAAT ATTAGTGAAA TATAAATAAA TTTGATTATC AAAATAATAA TTATAAATTA 601 GTCATTAACC AAAAAAAAAG AAAA Predicted gene structure (within gDNA segment 1 to 3201): Exon 1 1804 1846 ( 43 n); cDNA 184 225 ( 42 n); score: 0.791 Intron 1 1847 1915 ( 69 n); Pd: 0.000 (s: 0.79), Pa: 0.000 (s: 0.62) Exon 2 1916 2313 ( 398 n); cDNA 226 624 ( 399 n); score: 0.795 MATCH C06HBa0046G10.1-8+ SGN-E542827- 0.795 441 0.707 C PGS_C06HBa0046G10.1-8+_SGN-E542827- (1804 1846,1916 2313) Alignment (genomic DNA sequence = upper lines): TTAGGAAAAA AGGTTTGATA TACCACCCAA CTTTGTCATT TGGGTTTGAT ATATTCCTCG 1863 ||| | |||| | |||||||| || | ||| ||||||||| || TTACGGAAAA A-GTTTGATA TATTTCTCAA TTTTGTCATT TGC....... .......... 225 AATTGAAAGT GACTCATATA TACCTTTACT GTTATAGAAA TGACTCACAT ATACCCCTTT 1923 | | || | .......... .......... .......... .......... .......... ..AGCACTGT 233 CCTTACAAAA TGAGCTTACA TATACGCTTC ATTTAACGGA AGTTAAAAAA TTAGTTTTAA 1983 | |||||||| || ||| | | |||| | |||| | | | | ||| || CGTTACAAAA TG-GCTAATA TATATTAAAA AATTAATTTT AAATTTATAT TTA-TTACTT 291 ATTATTTTTT AAAAAAAAAA TGATTTAGGG GTATATATGA TTCTTCTATC AAAGTTCAAG 2043 | ||| ||| ||||||| | ||||| | |||||||| | |||||||||| ||||||| || TTGATTGTTT -TTAAAAAAA TTATTTAAAG GTATATATTA TTCTTCTATC AAAGTTCGAG 350 G-TATATTTA ATTTTTTTTC ATACACAAAT TATTTTTTGA CTTCTTTTAT TATAATCATT 2102 | |||||||| ||||||||| ||||| |||| |||| ||||| |||||||||| |||||| ||| GTTATATTTA ATTTTTTTT- ATACATAAAT TATTGTTTGA CTTCTTTTAT TATAATTATT 409 TGAGTTTCTT ATTCT-TTTT TTTTTCTTTC ATTCCTTAGT TT-AA-AGAA AAAAAATTAA 2159 ||||||| || ||||| ||| |||||||||| |||||||||| || || |||| |||||| ||| TGAGTTTGTT ATTCTAATTT TTTTTCTTTC ATTCCTTAGT TTAAAGAGAA AAAAAACTAA 469 ACTA--TTTT TT-TCTATAT TGTAATTTGA TTTTTGTATT CGAAGAAAAA AGTTTTATCA 2216 |||| |||| || | | ||| |||||||| | ||| ||||| | ||| |||| | ||| ||| ACTATTTTTT TTGTGTGTAT TGTAATTT-A ATTTGGTATT CAAAG-AAAA ATTTTGGTCA 527 TCTACAATAA GTTTTACAAG AATATTAGTG AAATATAAAT AAATTTAATT ATCAAAATAA 2276 |||||||||| |||||||||| |||||||||| |||||||||| |||||| ||| |||||||||| TCTACAATAA GTTTTACAAG AATATTAGTG AAATATAAAT AAATTTGATT ATCAAAATAA 587 TAATTTTAAA TTAGTCATTG AAAGAAAAAA AATAAAA 2313 ||||| |||| ||||||||| | |||||| || |||| TAATTATAAA TTAGTCATTA ACCAAAAAAA AAGAAAA 624 hqPGS_C06HBa0046G10.1-8+_SGN-E542827- (1804 1846,1916 2313) ******************************************************************************** EST sequence 1 -strand 460 n (File: SGN-E243215-) 1 GTATGAAATT TTAAAAAATA GGGAAAAGGG TCTGATATAC CTCTCAACTT TGTCATTTAG 61 AGCTGATATA TCCCTCGTAA TAAAAGTGGT TCATATATGC CCTTATCGTT ATACAAACGG 121 CTCACATATA CCCCTGCCGT TAGAAAATGG CTCACATATA CCCTTCATTT AACGAAAGTT 181 AAAAAATTAG TTTTAAATTT ATATTTGTTA CTTCTAATTT TTTTTTAAAA AAAATATTTA 241 GGGATATATA TGATTCTTCT ATCAAAGTTC AAGGTATATT TTAATTTTTT CATACAAAAA 301 TTATTTTTTG ACTTCTTTTA TTATAATAAT TTGAGTTTCT TATTCTTATT TTGTTTTTTT 361 CTTTCATTCC TTAGTTTAAA TAAAAAAAAT TCAAATATTT TTTTTGTGTG TATTGTAATT 421 TAATTTCGTA TTCGAAGAAA AAATTGGTCA TCTACAATAA Predicted gene structure (within gDNA segment 1 to 3546): Exon 1 1789 2226 ( 438 n); cDNA 3 460 ( 458 n); score: 0.729 MATCH C06HBa0046G10.1-8+ SGN-E243215- 0.729 438 0.952 C PGS_C06HBa0046G10.1-8+_SGN-E243215- (1789 2226) Alignment (genomic DNA sequence = upper lines): ATAAAATCAT TAAAGTTAGG AAAAAAGGTT TGATATACCA CCCAACTTTG TCATTTGGGT 1848 || |||| | ||| |||| |||| ||| ||||||||| | |||||||| |||||| | ATGAAATTTT AAAAAATAGG GAAAAGGGTC TGATATACCT CTCAACTTTG TCATTTAGAG 62 TTGATATATT CCTCGAATTG AAAGTGACTC ATATATACCT TTACTGTTAT AGAAATGACT 1908 |||||||| ||||| | | |||||| || |||||| || ||| ||||| | ||| | || CTGATATATC CCTCGTAATA AAAGTGGTTC ATATATGCCC TTATCGTTAT ACAAACGGCT 122 CACATATACC CCTTTCCTTA CAAAATGAGC TTACATATAC GCTTCATTTA ACGGAAGTTA 1968 |||||||||| ||| | ||| |||||| || | |||||||| ||||||||| ||| |||||| CACATATACC CCTGCCGTTA GAAAATG-GC TCACATATAC CCTTCATTTA ACGAAAGTTA 181 AAAAATTAGT TTTAAA-TTA T-TTT-TTA- ----AA---- -----AAAAA AATGATTTAG 2011 |||||||||| |||||| ||| | ||| ||| || ||||| || |||||| AAAAATTAGT TTTAAATTTA TATTTGTTAC TTCTAATTTT TTTTTAAAAA AAATATTTAG 241 GGGTATATAT GATTCTTCTA TCAAAGTTCA AGGTATATTT AATTTTTTTT CATACACAAA 2071 || ||||||| |||||||||| |||||||||| |||||||||| | |||||| |||||| ||| GGATATATAT GATTCTTCTA TCAAAGTTCA AGGTATATTT TA-ATTTTTT CATACAAAAA 300 TTATTTTTTG ACTTCTTTTA TTATAATCAT TTGAGTTTCT TATTC----T TT-TTTTTTT 2126 |||||||||| |||||||||| ||||||| || |||||||||| ||||| | || ||||||| TTATTTTTTG ACTTCTTTTA TTATAATAAT TTGAGTTTCT TATTCTTATT TTGTTTTTTT 360 CTTTCATTCC TTAGTTTAAA GAAAAAAAAT T-AAA-CTAT TTTTT-TCTA TATTGTAATT 2183 |||||||||| |||||||||| ||||||||| | ||| | | ||||| | | |||||||||| CTTTCATTCC TTAGTTTAAA TAAAAAAAAT TCAAATATTT TTTTTGTGTG TATTGTAATT 420 TGATTTTTGT ATTCGAAGAA AAAAGTTTTA TCATCTACAA TAA 2226 | | ||| || |||||||||| |||| || |||||||||| ||| T-AATTTCGT ATTCGAAGAA AAAA--TTGG TCATCTACAA TAA 460 hqPGS_C06HBa0046G10.1-8+_SGN-E243215- (1789 2226) Total number of EST alignments reported: 2 ________________________________________________________________________________ Predicted gene locations (1) in segment 1 to 5464: PGL 1 (+ strand): 1789 2313 AGS-1 (1789 2226) SCR (e 0.729) Exon 1 1789 2226 ( 438 n); score: 0.729 PGS (1789 2226) SGN-E243215- 3-phase translation of AGS-1 (+strand): . . . . . . 1789 ATAAAATCATTAAAGTTAGGAAAAAAGGTTTGATATACCACCCAACTTTGTCATTTGGGT I K S L K L G K K V - Y T T Q L C H L G - N H - S - E K R F D I P P N F V I W V K I I K V R K K G L I Y H P T L S F G . . . . . . 1849 TTGATATATTCCTCGAATTGAAAGTGACTCATATATACCTTTACTGTTATAGAAATGACT L I Y S S N - K - L I Y T F T V I E M T - Y I P R I E S D S Y I P L L L - K - L F D I F L E L K V T H I Y L Y C Y R N D . . . . . . 1909 CACATATACCCCTTTCCTTACAAAATGAGCTTACATATACGCTTCATTTAACGGAAGTTA H I Y P F P Y K M S L H I R F I - R K L T Y T P F L T K - A Y I Y A S F N G S - S H I P L S L Q N E L T Y T L H L T E V . . . . . . 1969 AAAAATTAGTTTTAAATTATTTTTTAAAAAAAAAATGATTTAGGGGTATATATGATTCTT K N - F - I I F - K K N D L G V Y M I L K I S F K L F F K K K M I - G Y I - F F K K L V L N Y F L K K K - F R G I Y D S . . . . . . 2029 CTATCAAAGTTCAAGGTATATTTAATTTTTTTTCATACACAAATTATTTTTTGACTTCTT L S K F K V Y L I F F H T Q I I F - L L Y Q S S R Y I - F F F I H K L F F D F F S I K V Q G I F N F F S Y T N Y F L T S . . . . . . 2089 TTATTATAATCATTTGAGTTTCTTATTCTTTTTTTTTTCTTTCATTCCTTAGTTTAAAGA L L - S F E F L I L F F F F H S L V - R Y Y N H L S F L F F F F S F I P - F K E F I I I I - V S Y S F F F L S F L S L K . . . . . . 2149 AAAAAAATTAAACTATTTTTTTCTATATTGTAATTTGATTTTTGTATTCGAAGAAAAAAG K K I K L F F S I L - F D F C I R R K K K K L N Y F F L Y C N L I F V F E E K S K K N - T I F F Y I V I - F L Y S K K K . . 2209 TTTTATCATCTACAATAA F Y H L Q - F I I Y N V L S S T I Maximal non-overlapping open reading frames (>= 64 codons): >C06HBa0046G10.1-8+_PGL-1_AGS-1_PPS_1 (1791 2006) (frame '0'; 213 bp, 71 residues) 1 KIIKVRKKGL IYHPTLSFGF DIFLELKVTH IYLYCYRNDS HIPLSLQNEL TYTLHLTEVK 61 KLVLNYFLKK K- 3-phase translation of AGS-1 (-strand): . . . . . . 2226 TTATTGTAGATGATAAAACTTTTTTCTTCGAATACAAAAATCAAATTACAATATAGAAAA L L - M I K L F S S N T K I K L Q Y R K Y C R - - N F F L R I Q K S N Y N I E K I V D D K T F F F E Y K N Q I T I - K . . . . . . 2166 AAATAGTTTAATTTTTTTTCTTTAAACTAAGGAATGAAAGAAAAAAAAAAGAATAAGAAA K - F N F F S L N - G M K E K K K N K K N S L I F F L - T K E - K K K K R I R N K I V - F F F F K L R N E R K K K E - E . . . . . . 2106 CTCAAATGATTATAATAAAAGAAGTCAAAAAATAATTTGTGTATGAAAAAAAATTAAATA L K - L - - K K S K N N L C M K K N - I S N D Y N K R S Q K I I C V - K K I K Y T Q M I I I K E V K K - F V Y E K K L N . . . . . . 2046 TACCTTGAACTTTGATAGAAGAATCATATATACCCCTAAATCATTTTTTTTTTAAAAAAT Y L E L - - K N H I Y P - I I F F L K N T L N F D R R I I Y T P K S F F F - K I I P - T L I E E S Y I P L N H F F F K K . . . . . . 1986 AATTTAAAACTAATTTTTTAACTTCCGTTAAATGAAGCGTATATGTAAGCTCATTTTGTA N L K L I F - L P L N E A Y M - A H F V I - N - F F N F R - M K R I C K L I L - - F K T N F L T S V K - S V Y V S S F C . . . . . . 1926 AGGAAAGGGGTATATGTGAGTCATTTCTATAACAGTAAAGGTATATATGAGTCACTTTCA R K G V Y V S H F Y N S K G I Y E S L S G K G Y M - V I S I T V K V Y M S H F Q K E R G I C E S F L - Q - R Y I - V T F . . . . . . 1866 ATTCGAGGAATATATCAAACCCAAATGACAAAGTTGGGTGGTATATCAAACCTTTTTTCC I R G I Y Q T Q M T K L G G I S N L F S F E E Y I K P K - Q S W V V Y Q T F F P N S R N I S N P N D K V G W Y I K P F F . . 1806 TAACTTTAATGATTTTAT - L - - F Y N F N D F L T L M I L Maximal non-overlapping open reading frames (>= 64 codons): none AGS-2 (1804 1846,1916 2313) SCR (e 0.791 d 0.000 a 0.000,e 0.795) Exon 1 1804 1846 ( 43 n); score: 0.791 Intron 1 1847 1915 ( 69 n); Pd: 0.000 Pa: 0.000 Exon 2 1916 2313 ( 398 n); score: 0.795 PGS (1804 1846,1916 2313) SGN-E542827- 3-phase translation of AGS-2 (+strand): . . . . . : . 1804 TTAGGAAAAAAGGTTTGATATACCACCCAACTTTGTCATTTGG : ACCCCTTTCCTTACAAA L G K K V - Y T T Q L C H L : D P F P Y K - E K R F D I P P N F V I W : T P F L T K R K K G L I Y H P T L S F G : P L S L Q . . . . . . 1933 ATGAGCTTACATATACGCTTCATTTAACGGAAGTTAAAAAATTAGTTTTAAATTATTTTT M S L H I R F I - R K L K N - F - I I F - A Y I Y A S F N G S - K I S F K L F F N E L T Y T L H L T E V K K L V L N Y F . . . . . . 1993 TAAAAAAAAAATGATTTAGGGGTATATATGATTCTTCTATCAAAGTTCAAGGTATATTTA - K K N D L G V Y M I L L S K F K V Y L K K K M I - G Y I - F F Y Q S S R Y I - L K K K - F R G I Y D S S I K V Q G I F . . . . . . 2053 ATTTTTTTTCATACACAAATTATTTTTTGACTTCTTTTATTATAATCATTTGAGTTTCTT I F F H T Q I I F - L L L L - S F E F L F F F I H K L F F D F F Y Y N H L S F L N F F S Y T N Y F L T S F I I I I - V S . . . . . . 2113 ATTCTTTTTTTTTTCTTTCATTCCTTAGTTTAAAGAAAAAAAATTAAACTATTTTTTTCT I L F F F F H S L V - R K K I K L F F S F F F F S F I P - F K E K K L N Y F F L Y S F F F L S F L S L K K K N - T I F F . . . . . . 2173 ATATTGTAATTTGATTTTTGTATTCGAAGAAAAAAGTTTTATCATCTACAATAAGTTTTA I L - F D F C I R R K K F Y H L Q - V L Y C N L I F V F E E K S F I I Y N K F Y Y I V I - F L Y S K K K V L S S T I S F . . . . . . 2233 CAAGAATATTAGTGAAATATAAATAAATTTAATTATCAAAATAATAATTTTAAATTAGTC Q E Y - - N I N K F N Y Q N N N F K L V K N I S E I - I N L I I K I I I L N - S T R I L V K Y K - I - L S K - - F - I S . . . 2293 ATTGAAAGAAAAAAAATAAAA I E R K K I K L K E K K - H - K K K N K Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Mon Aug 28 21:42:42 2006