<?xml version="1.0" encoding="ISO-8859-1"?>
<GTH_output xmlns="http://www.genomethreader.org/GTH_output/" GTH_XML_version="1.1">
  <header xmlns="http://www.genomethreader.org/GTH_output/header/">
    <source program="GenomeThreader" version="0.9.54" build_date="2006-07-28 11:55:15" run_date="2009-01-16 20:39:11"/>
    <gDNA_template_files>
      <temp_name>/tmp/bac-submission-temp-C06HBa0167M06-FMABi/GenomeThreader_SGN_markers/un_xed_seqs</temp_name>
    </gDNA_template_files>
    <reference_files>
      <file ref_name="/tmp/bac-submission-temp-C06HBa0167M06-FMABi/gth_cdna_fileCXGN::BACSubmission::Analysis::GenomeThreader_SGN_markers" type="ESTcDNA"/>
    </reference_files>
    <splice_site_parameters parameter_type="Bayesian" species="arabidopsis"/>
    <parameters>
      <parameter name="bssmfile" value="arabidopsis"/>
      <parameter name="scorematrixfile" value="BLOSUM62"/>
      <parameter name="searchmode" value="forward=True,reverse=True)"/>
      <parameter name="translationtable" value="1"/>
      <parameter name="frompos" value="0"/>
      <parameter name="topos" value="0"/>
      <parameter name="width" value="0"/>
      <parameter name="verbose" value="False"/>
      <parameter name="skipalignmentout" value="False"/>
      <parameter name="showintronmaxlen" value="120"/>
      <parameter name="minorflen" value="64"/>
      <parameter name="showseqnums" value="False"/>
      <parameter name="gs2out" value="False"/>
      <parameter name="maskpolyatails" value="False"/>
      <parameter name="noautoindex" value="False"/>
      <parameter name="minmatchlen" value="20"/>
      <parameter name="seedlength" value="16"/>
      <parameter name="exdrop" value="2"/>
      <parameter name="online" value="False"/>
      <parameter name="inverse" value="False"/>
      <parameter name="exact" value="False"/>
      <parameter name="chainwf" value="0.500000"/>
      <parameter name="gcmaxgapwidth" value="1000000"/>
      <parameter name="gcmincoverage" value="50"/>
      <parameter name="introncutout" value="False"/>
      <parameter name="autointroncutout" value="0"/>
      <parameter name="icinitialdelta" value="50"/>
      <parameter name="iciterations" value="2"/>
      <parameter name="icdeltaincrease" value="50"/>
      <parameter name="icminremintronlen" value="10"/>
      <parameter name="nou12intronmodel" value="False"/>
      <parameter name="u12donorprob" value="0.990000"/>
      <parameter name="u12donorprob1mism" value="0.900000"/>
      <parameter name="probies" value="0.500000"/>
      <parameter name="probdelgen" value="0.030000"/>
      <parameter name="identityweight" value="2.000000"/>
      <parameter name="mismatchweight" value="-2.000000"/>
      <parameter name="undetcharweight" value="0.000000"/>
      <parameter name="deletionweight" value="-4.000000"/>
      <parameter name="dpminexonlen" value="5"/>
      <parameter name="dpminintronlen" value="50"/>
      <parameter name="shortexonpenal" value="100"/>
      <parameter name="shortintronpenal" value="100"/>
      <parameter name="wzerotransition" value="80"/>
      <parameter name="wdecreasedoutput" value="80"/>
      <parameter name="leadcutoffsmode" value="RELAXED"/>
      <parameter name="termcutoffsmode" value="STRICT"/>
      <parameter name="cutoffsminexonlen" value="5"/>
      <parameter name="scoreminexonlen" value="50"/>
      <parameter name="minaveragessp" value="0.500000"/>
      <parameter name="minalignmentscore" value="0.900000"/>
      <parameter name="maxalignmentscore" value="1.000000"/>
      <parameter name="mincoverage" value="0.900000"/>
      <parameter name="maxcoverage" value="100.000000"/>
      <parameter name="intermediate" value="False"/>
      <parameter name="sortags" value="False"/>
      <parameter name="sortagswf" value="1.000000"/>
      <parameter name="first" value="0"/>
      <parameter name="exondistri" value="False"/>
      <parameter name="introndistri" value="False"/>
      <parameter name="refseqcovdistri" value="False"/>
    </parameters>
    <overall_reference_type>ESTcDNA</overall_reference_type>
  </header>
  <alignment_module>
  <spliced_alignment xmlns="http://www.genomethreader.org/GTH_output/alignment_module/spliced_alignment/">
    <reference ref_file="/tmp/bac-submission-temp-C06HBa0167M06-FMABi/gth_cdna_fileCXGN::BACSubmission::Analysis::GenomeThreader_SGN_markers" ref_id="SGN-M3094" ref_strand="+" ref_description="SGN-M3094 cLET-4-G23 [est_clones]">
      <seq>caatttttttttaaaaaaaaatgataaatttttcttcaagaattttgttgattttgttattaacatgtactatagctataattataattccaaaattggcttctacacaatattgtggatgtacagaaggattgtgttgcagtagatggggttattgtggcagtggaaaagcttattgtggacaaggttgtcaaggagggccttgttataattcttcaaccaaaaattacaatcataatattaataacaataataataataataccaatagagtttctgatattgttagtgaatcatttttcaatggaattgcaaatcaagctgcttcaaattgtgaaggcaaaggattttacacgagatctgtatttcttgaagctttaaagtcttatcctgaattcgcaactatcggtttttctgatgatgataataagcgcgaaa</seq>
    </reference>
    <gDNA_segment>
      <template temp_file="/tmp/bac-submission-temp-C06HBa0167M06-FMABi/GenomeThreader_SGN_markers/un_xed_seqs" temp_id="C06HBa0167M06.1" temp_strand="+" temp_description="C06HBa0167M06.1  AC211057.1 htgs_phase:1 submitted_to_sgn_as:C06HBa0167M06 sequenced_by:cbsg upload_account_name:netherlands [organism=Solanum lycopersicum]">
        <position start="84245" stop="85282"/>
      </template>
    </gDNA_segment>
    <predicted_gene_structure xmlns="http://www.genomethreader.org/GTH_output/alignment_module/spliced_alignment/">
      <exon-intron_info>
        <exon e_serial="1">
          <gDNA_exon_boundary g_start="84545" g_stop="84982" g_length="438"/>
          <reference_exon_boundary r_type="cDNA" r_start="1" r_stop="438" r_length="438" r_score="1.000"/>
        </exon>
      </exon-intron_info>
      <MATCH_line gen_id="C06HBa0167M06.1" gen_strand="+" ref_id="SGN-M3094" ref_strand="+">
        <total_alignment_score>1.000</total_alignment_score>
        <cumulative_length_of_scored_exons>438</cumulative_length_of_scored_exons>
        <coverage percentage="1.000" high_type="C"/>
      </MATCH_line>
      <PGS_line>
        <gDNA gen_id="C06HBa0167M06.1" gen_strand="+"/>
        <rDNA rDNA_id="SGN-M3094" rDNA_strand="+"/>
        <gDNA_exon_coordinates>
          <exon e_start="84545" e_stop="84982"/>
        </gDNA_exon_coordinates>
      </PGS_line>
      <alignment>
        <genome_strand>CAATTTTTTTTTAAAAAAAAATGATAAATTTTTCTTCAAGAATTTTGTTGATTTTGTTATTAACATGTACTATAGCTATAATTATAATTCCAAAATTGGCTTCTACACAATATTGTGGATGTACAGAAGGATTGTGTTGCAGTAGATGGGGTTATTGTGGCAGTGGAAAAGCTTATTGTGGACAAGGTTGTCAAGGAGGGCCTTGTTATAATTCTTCAACCAAAAATTACAATCATAATATTAATAACAATAATAATAATAATACCAATAGAGTTTCTGATATTGTTAGTGAATCATTTTTCAATGGAATTGCAAATCAAGCTGCTTCAAATTGTGAAGGCAAAGGATTTTACACGAGATCTGTATTTCTTGAAGCTTTAAAGTCTTATCCTGAATTCGCAACTATCGGTTTTTCTGATGATGATAATAAGCGCGAAA</genome_strand>
        <mrna_strand>CAATTTTTTTTTAAAAAAAAATGATAAATTTTTCTTCAAGAATTTTGTTGATTTTGTTATTAACATGTACTATAGCTATAATTATAATTCCAAAATTGGCTTCTACACAATATTGTGGATGTACAGAAGGATTGTGTTGCAGTAGATGGGGTTATTGTGGCAGTGGAAAAGCTTATTGTGGACAAGGTTGTCAAGGAGGGCCTTGTTATAATTCTTCAACCAAAAATTACAATCATAATATTAATAACAATAATAATAATAATACCAATAGAGTTTCTGATATTGTTAGTGAATCATTTTTCAATGGAATTGCAAATCAAGCTGCTTCAAATTGTGAAGGCAAAGGATTTTACACGAGATCTGTATTTCTTGAAGCTTTAAAGTCTTATCCTGAATTCGCAACTATCGGTTTTTCTGATGATGATAATAAGCGCGAAA</mrna_strand>
      </alignment>
    </predicted_gene_structure>
  </spliced_alignment>
    <total_number_ESTs_reported>1</total_number_ESTs_reported>
    </alignment_module>
  <PGL_module xmlns="http://www.genomethreader.org/GTH_output/PGL_module/">
    <predicted_gene_location>
      <PGL_line PGL_serial="1" PGL_strand="+" PGL_start="84545" PGL_stop="84982"/>
      <AGS_information>
        <AGS_line AGS_serial="1">
          <exon_coordinates>
            <exon e_start="84545" e_stop="84982"/>
          </exon_coordinates>
        </AGS_line>
        <SCR_line>
          <exon-only e_score="1.000"/>
        </SCR_line>
        <exon-intron_info xmlns="http://www.genomethreader.org/GTH_output/PGL_module/predicted_gene_location/AGS_information/exon-intron_info/">
          <exon e_serial="1" e_score="1.000">
            <gDNA_exon_boundary e_start="84545" e_stop="84982" e_length="438"/>
          </exon>
        </exon-intron_info>
        <supporting_evidence xmlns="http://www.genomethreader.org/GTH_output/PGL_module/predicted_gene_location/AGS_information/supporting_evidence/">
          <PGS_line>
            <gDNA_exon_coordinates>
              <exon start="84545" stop="84982"/>
            </gDNA_exon_coordinates>
            <referenceDNA id="SGN-M3094" strand="+"/>
          </PGS_line>
        </supporting_evidence>
        <three_phase_translation xmlns="http://www.genomethreader.org/GTH_output/PGL_module/predicted_gene_location/AGS_information/three_phase_translation/">
          <description PGL_serial="1" AGS_serial="1" gDNA_strand="+"/>
          <translation>
            <gDNA_template>CAATTTTTTTTTAAAAAAAAATGATAAATTTTTCTTCAAGAATTTTGTTGATTTTGTTATTAACATGTACTATAGCTATAATTATAATTCCAAAATTGGCTTCTACACAATATTGTGGATGTACAGAAGGATTGTGTTGCAGTAGATGGGGTTATTGTGGCAGTGGAAAAGCTTATTGTGGACAAGGTTGTCAAGGAGGGCCTTGTTATAATTCTTCAACCAAAAATTACAATCATAATATTAATAACAATAATAATAATAATACCAATAGAGTTTCTGATATTGTTAGTGAATCATTTTTCAATGGAATTGCAAATCAAGCTGCTTCAAATTGTGAAGGCAAAGGATTTTACACGAGATCTGTATTTCTTGAAGCTTTAAAGTCTTATCCTGAATTCGCAACTATCGGTTTTTCTGATGATGATAATAAGCGCGAAA</gDNA_template>
            <first_frame> Q  F  F  F  K  K  K  *  *  I  F  L  Q  E  F  C  *  F  C  Y  *  H  V  L  *  L  *  L  *  F  Q  N  W  L  L  H  N  I  V  D  V  Q  K  D  C  V  A  V  D  G  V  I  V  A  V  E  K  L  I  V  D  K  V  V  K  E  G  L  V  I  I  L  Q  P  K  I  T  I  I  I  L  I  T  I  I  I  I  I  P  I  E  F  L  I  L  L  V  N  H  F  S  M  E  L  Q  I  K  L  L  Q  I  V  K  A  K  D  F  T  R  D  L  Y  F  L  K  L  *  S  L  I  L  N  S  Q  L  S  V  F  L  M  M  I  I  S  A  K </first_frame>
            <second_frame>  N  F  F  L  K  K  N  D  K  F  F  F  K  N  F  V  D  F  V  I  N  M  Y  Y  S  Y  N  Y  N  S  K  I  G  F  Y  T  I  L  W  M  Y  R  R  I  V  L  Q  *  M  G  L  L  W  Q  W  K  S  L  L  W  T  R  L  S  R  R  A  L  L  *  F  F  N  Q  K  L  Q  S  *  Y  *  *  Q  *  *  *  *  Y  Q  *  S  F  *  Y  C  *  *  I  I  F  Q  W  N  C  K  S  S  C  F  K  L  *  R  Q  R  I  L  H  E  I  C  I  S  *  S  F  K  V  L  S  *  I  R  N  Y  R  F  F  *  *  *  *  *  A  R   </second_frame>
            <third_frame>   I  F  F  *  K  K  M  I  N  F  S  S  R  I  L  L  I  L  L  L  T  C  T  I  A  I  I  I  I  P  K  L  A  S  T  Q  Y  C  G  C  T  E  G  L  C  C  S  R  W  G  Y  C  G  S  G  K  A  Y  C  G  Q  G  C  Q  G  G  P  C  Y  N  S  S  T  K  N  Y  N  H  N  I  N  N  N  N  N  N  N  T  N  R  V  S  D  I  V  S  E  S  F  F  N  G  I  A  N  Q  A  A  S  N  C  E  G  K  G  F  Y  T  R  S  V  F  L  E  A  L  K  S  Y  P  E  F  A  T  I  G  F  S  D  D  D  N  K  R  E  </third_frame>
          </translation>
          <probable_ORFs xmlns="http://www.genomethreader.org/GTH_output/PGL_module/predicted_gene_location/AGS_information/three_phase_translation/probable_ORFs/">
            <orf_entry>
              <id_line>
                <gDNA id="C06HBa0167M06.1" strand="+"/>
                <serials PGL_serial="1" AGS_serial="1" PPS_serial="1"/>
                <orf_info>
                  <exon_boundaries>
                    <exon start="84559" stop="84981"/>
                  </exon_boundaries>
                  <frame>2</frame>
                  <number_coding_nucleotides>423</number_coding_nucleotides>
                  <number_encoded_amino_acids>141</number_encoded_amino_acids>
                </orf_info>
              </id_line>
              <predicted_protein_sequence>KKMINFSSRILLILLLTCTIAIIIIPKLASTQYCGCTEGLCCSRWGYCGSGKAYCGQGCQGGPCYNSSTKNYNHNINNNNNNNTNRVSDIVSESFFNGIANQAASNCEGKGFYTRSVFLEALKSYPEFATIGFSDDDNKRE</predicted_protein_sequence>
            </orf_entry>
          </probable_ORFs>
        </three_phase_translation>
      </AGS_information>
    </predicted_gene_location>
  </PGL_module>
</GTH_output>
<!--
$ general statistics:
$ 4 chains have been computed
$ 
$ memory statistics:
$ 2040 bytes spliced alignments in total
$ 1 spliced alignments have been stored
$ 2040 bytes was the average size of a spliced alignment
$ 5528 bytes predicted gene locations in total
$ 1 predicted gene locations have been stored
$ 5528 bytes was the average size of a predicted gene location
$ 0 megabytes was the average size of the backtrace matrix
$ 4 backtrace matrices have been allocated
$ 
$ date finished: 2009-01-16 20:39:15
-->
