GeneSeqer. Version of March 12, 2006. Date run: Mon Aug 28 23:49:22 2006 (Bayesian) Splice site model (species): Arabidopsis thaliana Fast search parameters: MinMatchLen 16, MinQualityHSP 30, MinQualityCHAIN 50. Total number of ESTs: 34829 Total sequence length: 35392868 Minimum sequence length: 63 Maximum sequence length: 5381 Length distribution (number of sequences of specified length): < 100: 4 < 200: 53 < 300: 143 < 400: 353 < 500: 791 < 600: 1674 < 700: 2583 < 800: 4023 < 900: 6481 < 1000: 5706 >=1000: 13018 Input file : /tmp/bac-submission-temp-5hdNe/C08HBa0217O07/C08HBa0217O07.seq.screen ________________________________________________________________________________ Sequence 1: C08HBa0217O07.1, from 1 to 36199, both strands analyzed. ... started at: Tue Aug 29 00:00:58 2006 EST library file: /tmp/cxgn-bacpublish-resources-5XcGm4/lycopersicum_combined_unigene_seqs; matching gDNA +strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 4 EST library file: /tmp/cxgn-bacpublish-resources-5XcGm4/lycopersicum_combined_unigene_seqs; matching gDNA -strand ... ... found all matches, elapsed seconds = 2 ... matches indexed, elapsed seconds = 2 HitsTableSize = 7 ******************************************************************************** EST sequence 1 +strand 1138 n (File: SGN-U327248+) 1 GAAGAACTGG AATAGGATCT GATTTGTTAT CCTCCTTCCT TCTCCTTACA AGACACAGAT 61 CAGCTGAATT GAAACGAAAT TAGTGATTAA AATTCCCCTG TAAGAGGCTA TCTCTGATTT 121 ACAATGGAAC TTCATGAACT TCTGGCACGA TCTTCGACAA TGGTCATCGC AGTGATACTA 181 ATTGCTAGGA TCAGCTCATG TCTCAATACT GCAGTATTAA GTGAAGTGCA TGCACCTTAT 241 GTTGATAATT ATGTGTCAAA ATCCTACTTT AACAAACATG ACAATTTGGT AGATTCAAAG 301 TTTGAAGATT TCATTGCACA TGACATATTG TACGGTGCAT GTGAATCACT GCAAGGAAAC 361 GTCGAATTTT CACCAAAACT GTCTGCTTTA CATGGAAAGC TGATTGGTGA AGGCTCCCAT 421 CGGCGTCTGT CATCAACTCT CAGATTGAAG ATGTCATCAG AGTCCATTTC CATGCCTCCA 481 AAATCTTGTG AGGTTATAAT TGTTGAAAGA CTGCCTTCTG GAGTCTTTGC AGATCCTTTT 541 GAGCTACAAC ACCTTGTTCA GCGTGGTGGT AAATTACTAA GTATTAGATT TTATGGGTTT 601 CTAACACTGC TACCCGTCAT TTTGAGGCTT GTTGTTGTCC ACGTTTTGTT GTAGTTTTCA 661 GGGATGCTGC CGTATTTGGA GATACAAATT TAGAGTTGCC TTCTTTTCTG TCAAACCGCT 721 CGCTTGTTGA GGTTCATCTG GAAGTGGGTA CCAATTTAAC ATCACAACAA AAGGATGAAC 781 TGGAAATACA CATGGAACTT CCTCTACATG CACGCTATCA GCCACTAGGA CATGGATTCT 841 CAAGAGTTCA ATTTGCTTCA CCTGACTTAT TTTTGCGCTG CAACATTAAA AGAAAAGAAG 901 ATGACACGGG CTGTTTGTTT CTGCTTGACA AACAGAATGC TGAATCAATT GATACTCACC 961 CTGTGTGGGA AGTACCATGT GGAAATCGAG AACACACTGA AGTTGTATCT GCTTTTACAT 1021 TCATCTCAGC TGTTTTATCA GCTCTTCTCA TCATTGTTGC CTCAATCAAG TACTCTGATA 1081 ATGCATCTAA TAATATCTTA AAACAATCAT GAGTAATTGA TAAAAAAAAA AAAAAAAA Predicted gene structure (within gDNA segment 1 to 2144): Exon 1 131 369 ( 239 n); cDNA 583 821 ( 239 n); score: 0.992 Intron 1 370 569 ( 200 n); Pd: 0.999 (s: 1.00), Pa: 0.814 (s: 0.98) Exon 2 570 817 ( 248 n); cDNA 822 1069 ( 248 n); score: 0.964 PPA cDNA 1120 1138 MATCH C08HBa0217O07.1+ SGN-U327248+ 0.977 487 0.428 C PGS_C08HBa0217O07.1+_SGN-U327248+ (131 369,570 817) Alignment (genomic DNA sequence = upper lines): ATTAGATTTT ATGGGTTTCT AGCACTGCTA CCCGTCATTT TGAGGCTTGT TGTTGTCCAC 190 |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| ATTAGATTTT ATGGGTTTCT AACACTGCTA CCCGTCATTT TGAGGCTTGT TGTTGTCCAC 642 GTTTTGTTGT AGTTTTCAGG GATGCTGCCG TATTTGGAGA TACAAATTTA GAGTTGCCTT 250 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTGTTGT AGTTTTCAGG GATGCTGCCG TATTTGGAGA TACAAATTTA GAGTTGCCTT 702 CTTTTCTGTC AAACCGCTCG CTTGTTGAGG TTCATCTGGA AGTGGGTACC AATTTAACAT 310 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTTTCTGTC AAACCGCTCG CTTGTTGAGG TTCATCTGGA AGTGGGTACC AATTTAACAT 762 CACAACAGAA GGATGAACTG GAAATACACA TGGAACTTCC TCTACATGCA CGCTATCAGG 370 ||||||| || |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| CACAACAAAA GGATGAACTG GAAATACACA TGGAACTTCC TCTACATGCA CGCTATCAG. 821 TACATCACAT AAATAATTCC CAAAATTATG TCGGAGTCTC TAAAATATGT TCCAGGCATC 430 .......... .......... .......... .......... .......... .......... 821 CAGTGTAGTC AGTCATTTTT ACTCCCCTGA AATTTAAGTT GATACTTGTA CAAGAAAAGA 490 .......... .......... .......... .......... .......... .......... 821 TGCATTAAGT ATGTAGGAAG CCATGAATAT CTGTTAAGCT TCTCGACTTT ATTGTAACAT 550 .......... .......... .......... .......... .......... .......... 821 CCCGGCTTCT AACTGGCAGC CACTAGGACA TGGATTCTCA AGAGTTGAAT TTGCTTCACC 610 | |||||||||| |||||||||| |||||| ||| |||||||||| .......... .........C CACTAGGACA TGGATTCTCA AGAGTTCAAT TTGCTTCACC 862 TGACTTATTT CTGCGCTGCA ACCTTGAAAG AAAAGAATAT GACACGGGCT GTTTGTTTCT 670 |||||||||| ||||||||| || || |||| ||||||| || |||||||||| |||||||||| TGACTTATTT TTGCGCTGCA ACATTAAAAG AAAAGAAGAT GACACGGGCT GTTTGTTTCT 922 GCTAGACAAA CAGAATGCTG AATCAATTGA TACTCACCCT GTGTGGGAAG TACCATGTGG 730 ||| |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTGACAAA CAGAATGCTG AATCAATTGA TACTCACCCT GTGTGGGAAG TACCATGTGG 982 AAATCGAGAA CACACTGAAG TTGTATCTGC TTTTACATTC ATCTCAGCTC TTGTATCAGC 790 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| || ||||||| AAATCGAGAA CACACTGAAG TTGTATCTGC TTTTACATTC ATCTCAGCTG TTTTATCAGC 1042 TCTTCTCATC ATTGTTGCCT CAATTAA 817 |||||||||| |||||||||| |||| || TCTTCTCATC ATTGTTGCCT CAATCAA 1069 hqPGS_C08HBa0217O07.1+_SGN-U327248+ (131 369,570 817) ******************************************************************************** EST sequence 11 +strand 977 n (File: SGN-U331953+) 1 TTGGCTAATA TCAAGTGTAG TATCTGTTCT TATCAGTTTA ATATCTGATA TGTGAATCAA 61 TGATTCACAC GATATTAACT CAATTTTTTA AGGGGAGAGT TCATCAAGGT AGCTTGCTAC 121 TTGGGCTCTC GTGTGTCGCC TATGCGTTGC ACTACTGCAT GGGCCTGGCA CACCCCACCA 181 AATCTACTTC AAATCTTTTG GTTGATATAT TGTGTAATGT AATCTAATTC AAATCTTTCG 241 GTTGATATAT TGTGTAATGT GTTCTTATTA TGTTTATACA TACTTATATT TATTTATTTA 301 ATTTTATATA ATTTTAAATG TCGTGTATGG GCATAAGCTA AGTTAGATTT ACAGCGAAAT 361 ATAATTTTGG TTAAAATTGA ACACTGTTTT TAGATCTTGT TTGACAATTT GAAAGTTAAC 421 AATGAGTATT GTTGTGGCTT TGCTAATATT GTTTTTCAGT AGTAATCTCC TTTGATGAAT 481 TGATTGAGTT GCTTCACTTC TCCAGTTTGT TGATCCACCA CTGCTTTGAC AATATCCGCC 541 ACAGAAATCA TGCCTACTAC CTTCCCATCG ATCACTGGAA CATGTCGTAT GTGGTGCTCT 601 GTGATCATAA AACGTTCCAT CACATTTCCA TAGTGCATTA AGCATCAGTA ACTTCGCGAG 661 TTAGAACTAA TACTTACCAC TCATGAGCTG CATTGCTTGA AGAATGTTTG TGTCCGATGT 721 GACGGTTATC AGCTTGCTCT GTTGCAATGG TAGTACTAAT ATTTGAGCAC AAAACAGAAG 781 ACAGATGAAA AAAAGATAAA GCCAGGAAGA CCGATATCTA TAGAGTACCT GCTCGGTCAT 841 AATATCTCCG ACTCTTGTGT ATTTAGATGA TCTATCCTGT ACAATTACCT TTCGCAAATA 901 ATCTGTACGT CTCTTGTACA ATGTGCATAG ATGAATAAGT TCAACATTGT GTGTATTACT 961 AAAGATCAAA AAAAAAA Predicted gene structure (within gDNA segment 2821 to 1): Exon 1 2140 1959 ( 182 n); cDNA 1 181 ( 181 n); score: 0.912 Intron 1 1958 286 (1673 n); Pd: 0.665 (s: 0.90), Pa: 0.842 (s: 0) Exon 2 285 275 ( 11 n); cDNA 182 192 ( 11 n); score: 0.727 PPA cDNA 968 977 MATCH C08HBa0217O07.1- SGN-U331953+ 0.912 193 0.198 C PGS_C08HBa0217O07.1-_SGN-U331953+ (2140 1959,285 275) Alignment (genomic DNA sequence = upper lines): TTGGCTAAGA TCAAGTGTAG TATCTGTTCT TATCAGTTTA ATATCTGATA TGTGGACCAT 2081 |||||||| | |||||||||| |||||||||| |||||||||| |||||||||| |||| | || TTGGCTAATA TCAAGTGTAG TATCTGTTCT TATCAGTTTA ATATCTGATA TGTGAATCAA 60 TGGTTCACAT GATATTAACT CAATTTTTTA AGGGGAAGAG TCCATCAAGG TAGCTTGCTG 2021 || |||||| |||||||||| |||||||||| ||||| |||| | |||||||| ||||||||| TGATTCACAC GATATTAACT CAATTTTTTA AGGGG-AGAG TTCATCAAGG TAGCTTGCTA 119 CCTGGGCTCT TGCGCGTCGC CCATGCGTTG CACTACTGCA CGGGCCTGGC GCACCCCACC 1961 | |||||||| | | ||||| | |||||||| |||||||||| ||||||||| ||||||||| CTTGGGCTCT CGTGTGTCGC CTATGCGTTG CACTACTGCA TGGGCCTGGC ACACCCCACC 179 AAGTCTAGTA CAATTTTATT ACAATTTGAT GAGATCTCAT TTGTTTCGAA TGTCTTAAAT 1901 || AA........ .......... .......... .......... .......... .......... 181 TTTGAGTATT TTGATAGCAA AGTGCTTGAG TAGTCTAAGT TTTGCAATCA CTTTCTTATT 1841 .......... .......... .......... .......... .......... .......... 181 GTGCATACAG TAACTTTAGA AGGCATCACC ACTAAGAATC AATAATAGAT AAATACAATC 1781 .......... .......... .......... .......... .......... .......... 181 AGATACAGCA GCCAAATCTA TGAGTTATGA ACATGCATCA CCAGTAAAAG CTGAAACAGA 1721 .......... .......... .......... .......... .......... .......... 181 TCCGATAAGC TTTGAGAAAT GAGGTGAAAA CCAAACGCAA TCTATAAACA TTAACCAGCA 1661 .......... .......... .......... .......... .......... .......... 181 ATAAAGCTGA AACAGATCCA ATATGCTCGA GAAATGAGAT GAAAACAAAA TGGACATTTC 1601 .......... .......... .......... .......... .......... .......... 181 AAACAGATCA CGTTGTCAAA TAGCAATCTT TTGGAGGCAC GTACACAGCT AGAAACAAAC 1541 .......... .......... .......... .......... .......... .......... 181 ACTATGTTAG GTTCAAATCT ACAAATCCTG GAAACAGCAA CAACACGTAA ACATGATTCC 1481 .......... .......... .......... .......... .......... .......... 181 AGCTGAAACA ACCCCCCTCC TCCCCCCCCC CCCCCATACA CACACACTTT GTGCAAGGTA 1421 .......... .......... .......... .......... .......... .......... 181 TTGGGGTTGA GTCCCACATC AGCTCTTGAG CTGTGGGGGA ACTTGAATAA CCCTGGGTTC 1361 .......... .......... .......... .......... .......... .......... 181 TCCCATCTGT AATTACGAGC TTTTAGGGTG TGATAATCTC AAGGTTGTGT CAAAAAAGAT 1301 .......... .......... .......... .......... .......... .......... 181 GGATTAAAAG GAGCAGTTAA CTAGGGTTAT CATTATTTTT CTTGTCTGCT TTTTGAGTTA 1241 .......... .......... .......... .......... .......... .......... 181 TGGTAAAAAC ACCACTGGTT ATTGAGACGA ATAGAAAACG CAGGCAAAAT GGCAGACAAC 1181 .......... .......... .......... .......... .......... .......... 181 ATAGATTTTG GTCTTTGCTG TAACATAGCA TTTGCAAGTT CCCAACCTTC TCAAACATCA 1121 .......... .......... .......... .......... .......... .......... 181 TTCCAGATAT TAATTTGTAT ACAGAAAATT CTAACTAAAA ACAATACACA TGTTATGATT 1061 .......... .......... .......... .......... .......... .......... 181 TTGCCAGATA ATTGGATTCA AATCAGTGAC CAACAGCAAA AACAATGTCT TCACATAATA 1001 .......... .......... .......... .......... .......... .......... 181 GTTGATTTGA GAAATTAGTA AATGGCAAAA GCAGCAAAGC ATTTCAAAAC ATAGTAAGCA 941 .......... .......... .......... .......... .......... .......... 181 ACGGCCTAAA CTGATAAAGG TTAGTGAGGC TCTCTTGCCA ATGGTTAGTC CAAAAGGGGG 881 .......... .......... .......... .......... .......... .......... 181 AAAGAAAAAA CTTGCTACAT TTGTTAATTA CTCATGATTG TTTAAAGACA TTACTAGATG 821 .......... .......... .......... .......... .......... .......... 181 CATTTAATTG AGGCAACAAT GATGAGAAGA GCTGATACAA GAGCTGAGAT GAATGTAAAA 761 .......... .......... .......... .......... .......... .......... 181 GCAGATACAA CTTCAGTGTG TTCTCGATTT CCACATGGTA CTTCCCACAC AGGGTGAGTA 701 .......... .......... .......... .......... .......... .......... 181 TCAATTGATT CAGCATTCTG TTTGTCTAGC AGAAACAAAC AGCCCGTGTC ATATTCTTTT 641 .......... .......... .......... .......... .......... .......... 181 CTTTCAAGGT TGCAGCGCAG AAATAAGTCA GGTGAAGCAA ATTCAACTCT TGAGAATCCA 581 .......... .......... .......... .......... .......... .......... 181 TGTCCTAGTG GCTGCCAGTT AGAAGCCGGG ATGTTACAAT AAAGTCGAGA AGCTTAACAG 521 .......... .......... .......... .......... .......... .......... 181 ATATTCATGG CTTCCTACAT ACTTAATGCA TCTTTTCTTG TACAAGTATC AACTTAAATT 461 .......... .......... .......... .......... .......... .......... 181 TCAGGGGAGT AAAAATGACT GACTACACTG GATGCCTGGA ACATATTTTA GAGACTCCGA 401 .......... .......... .......... .......... .......... .......... 181 CATAATTTTG GGAATTATTT ATGTGATGTA CCTGATAGCG TGCATGTAGA GGAAGTTCCA 341 .......... .......... .......... .......... .......... .......... 181 TGTGTATTTC CAGTTCATCC TTCTGTTGTG ATGTTAAATT GGTACCCACT TCCAGATGAA 281 || | .......... .......... .......... .......... .......... .....ATCTA 186 CCTCAA 275 | |||| CTTCAA 192 hqPGS_C08HBa0217O07.1-_SGN-U331953+ (2140 1959) ******************************************************************************** EST sequence 5 +strand 1480 n (File: SGN-U315215+) 1 GCACGAGCTC AATTCACGCC CAATTTTCTC TCTCTTCCCT TACCCCAAAG GTTCCATCTC 61 TTTGCCCTCT TTCCAAATCT CCCAAATCCC CCAAAATTCT AGGGTTTTCA TTTCCCTGTT 121 GAAATTTGGA TCGGTGCTTT GTTTTAGTTT TTGTGTAGAG GTTCCGATTC CTGGATTTTG 181 TGGGAGAATT TTGGGGGATT CCAATTGTAT ATTAAGAGAA AGAGATGAGT TTTCAAGATC 241 TTGAAGCGGG TCGACCATTG GGGCCCCGTA GAGGGTATAT GAATGGTAAG CAAGACCCAA 301 CTCAAGCTGT GGCTTCTGGA ATTTTCCAGA TTAACACTGC TGTGTCAACG TTTCAAAGGC 361 TGGTCAATAC ACTTGGAACA CCCAAAGATA CACCCGAGCT GCGTGAGAAG TTGCACAAGA 421 CGAGGGTACA TATTGGGCAG TTAGTGAAAG ATACATCTGC AAAACTTAAG CAAGCCAGCG 481 AAACAGATCA TCGTGTTGAA GTCAGTGCTA GCAAGAAAAT AACAGATGCT AAACTTGCTA 541 AAGATTTTCA AGCAGTCTTG AAGGAGTTTC AAAAGGCTCA AAGGCTTGCA GCTGAGAGAG 601 AGACAGCATA TACACCTTTT GTTCCCCAAG CAGTTCTTCC TTCAAGTTAT ACAGCCAGTG 661 AGATAGATGT TTCTTCGGGA AAGAGTCCAG AACAGCGAGC TCTCCTTGTG GAATCAAGAA 721 GACAAGAGGT TTTACTTTTG GACAACGAGA TAGCCTTTAA TGAAGCAATC ATCGAAGAAA 781 GAGAGCAGGG AATACAAGAA ATACAACAAC AAATTGGTGA AGTGAATGAA ATTTTCAAGG 841 ACCTTGCTGT GCTTGTTCAT GAGCAAGGAG CTATGATTGA TGATATTGGT TCTAACGTTG 901 AGAATGCTCA TGCTGCGACT GCACAGGGGA GATCGCAACT TGCTAAAGCT GCAAAGACCC 961 AAAGATCAAA TTCATCTCTG ACTTGCTTAC TCCTGGTGAT TTTCGGAATC GTGCTCCTCA 1021 TTGTGATCGT AGTACTTGCA GCCTGAGCTG AAGATTACTG GGATATAGAG AAGGGTCCCG 1081 AACTCTGTTC AGACACAGAA GTGAGTGCTT TGATTCGAAA AAAAGTGACT GTTATGATTC 1141 GAGTCATTTC AATAGGCATC CTGGAGTGTG GCTCGGTTGT CCCAACCCTT GTTCTTCTTG 1201 TCATGGTTGA TAAGTCGTGT AGTTAGGGAG GTGGGATAAG AAAGGTTCCT GTTCAACTTG 1261 TAAACCTGGT GCTGTGTTTG CAAGATGTAT TGCTGACATG TATTGGTGGG TCTGTCAATA 1321 ACAGTTTATT TTTGTTTGAG GAGCAATCTC TTATATTTGC CCTTTGGATA CTCTTGAACC 1381 AGATGACATT CAATTCTTTA TTGATGCTTG TGTGTGTGTG AAGTTATCAC TAATGGGATA 1441 TGTTGATTTG TTTAAAAAAA AAAGGGGATT ATCTTGCATG Predicted gene structure (within gDNA segment 8013 to 2248): Exon 1 7343 6939 ( 405 n); cDNA 8 412 ( 405 n); score: 1.000 Intron 1 6938 5616 (1323 n); Pd: 0.983 (s: 1.00), Pa: 0.923 (s: 1.00) Exon 2 5615 5522 ( 94 n); cDNA 413 506 ( 94 n); score: 1.000 Intron 2 5521 5292 ( 230 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 5291 5152 ( 140 n); cDNA 507 646 ( 140 n); score: 1.000 Intron 3 5151 4759 ( 393 n); Pd: 1.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 4 4758 4684 ( 75 n); cDNA 647 721 ( 75 n); score: 1.000 Intron 4 4683 4484 ( 200 n); Pd: 0.999 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 4483 4326 ( 158 n); cDNA 722 879 ( 158 n); score: 1.000 Intron 5 4325 4246 ( 80 n); Pd: 0.996 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 6 4245 4145 ( 101 n); cDNA 880 980 ( 101 n); score: 1.000 Intron 6 4144 3592 ( 553 n); Pd: 0.974 (s: 1.00), Pa: 0.917 (s: 1.00) Exon 7 3591 3092 ( 500 n); cDNA 981 1480 ( 500 n); score: 0.980 MATCH C08HBa0217O07.1- SGN-U315215+ 0.993 1473 0.995 C PGS_C08HBa0217O07.1-_SGN-U315215+ (7343 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4145,3591 3092) Alignment (genomic DNA sequence = upper lines): CTCAATTCAC GCCCAATTTT CTCTCTCTTC CCTTACCCCA AAGGTTCCAT CTCTTTGCCC 7284 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAATTCAC GCCCAATTTT CTCTCTCTTC CCTTACCCCA AAGGTTCCAT CTCTTTGCCC 67 TCTTTCCAAA TCTCCCAAAT CCCCCAAAAT TCTAGGGTTT TCATTTCCCT GTTGAAATTT 7224 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTTCCAAA TCTCCCAAAT CCCCCAAAAT TCTAGGGTTT TCATTTCCCT GTTGAAATTT 127 GGATCGGTGC TTTGTTTTAG TTTTTGTGTA GAGGTTCCGA TTCCTGGATT TTGTGGGAGA 7164 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGATCGGTGC TTTGTTTTAG TTTTTGTGTA GAGGTTCCGA TTCCTGGATT TTGTGGGAGA 187 ATTTTGGGGG ATTCCAATTG TATATTAAGA GAAAGAGATG AGTTTTCAAG ATCTTGAAGC 7104 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTTGGGGG ATTCCAATTG TATATTAAGA GAAAGAGATG AGTTTTCAAG ATCTTGAAGC 247 GGGTCGACCA TTGGGGCCCC GTAGAGGGTA TATGAATGGT AAGCAAGACC CAACTCAAGC 7044 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGGTCGACCA TTGGGGCCCC GTAGAGGGTA TATGAATGGT AAGCAAGACC CAACTCAAGC 307 TGTGGCTTCT GGAATTTTCC AGATTAACAC TGCTGTGTCA ACGTTTCAAA GGCTGGTCAA 6984 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGGCTTCT GGAATTTTCC AGATTAACAC TGCTGTGTCA ACGTTTCAAA GGCTGGTCAA 367 TACACTTGGA ACACCCAAAG ATACACCCGA GCTGCGTGAG AAGTTGTAAG CTCTCGAAAT 6924 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TACACTTGGA ACACCCAAAG ATACACCCGA GCTGCGTGAG AAGTT..... .......... 412 TTTGTACGAT AAATTGAAGT TGTAAGCTGA ATTTTTGGAT GAAATTTTGC AATTTTTAAT 6864 .......... .......... .......... .......... .......... .......... 412 ACAGTTGGAA TTGCTTACAT TTCTTGTTGA ATTTGATGTT TTGTGGACTT ACTGTATTGT 6804 .......... .......... .......... .......... .......... .......... 412 CAATTGATGG ATTAATTCTG AGACAAGTTT ATAGCTTTTG TGGTGATTGA GGTCGCACTT 6744 .......... .......... .......... .......... .......... .......... 412 TAGCCATAAT CATTTATTGA GATAGACCGG GTCAACTGAT ACTGTTTTGT GGTATTTTTG 6684 .......... .......... .......... .......... .......... .......... 412 TTAGGCGATT TCATCTTTTA CTGTGAATTC GTGAATTTAT AACTCTTCCT GTATTAGTTT 6624 .......... .......... .......... .......... .......... .......... 412 TAGCGTACAC ACAGAGAGTC AGAAAGAGCA TTATACTTGA AACTGATGTT CTGCATTTGA 6564 .......... .......... .......... .......... .......... .......... 412 GGAAACTCTT TAGGAAGGCC TCAGCACATG GGCACACACT AAAAATGATG TCTAAATTAT 6504 .......... .......... .......... .......... .......... .......... 412 CACTGAACAT GTGCAAATGA GTTCTTCTGA TCACTTTGAG AGTTTTGATA TAGAGAAAAT 6444 .......... .......... .......... .......... .......... .......... 412 CAGTAAGCTT GCAAAAATAT TATAAATCAA AATTTTAGAA AACCATTTTC AGTTTTTTTG 6384 .......... .......... .......... .......... .......... .......... 412 TCCACCTGTA CTTCGTGTGG ATGACTGGGA AGTATATATT GTTCAGTATA TGGTTCTGAT 6324 .......... .......... .......... .......... .......... .......... 412 GAATGATAAA ACATGTTTTT AGGCTAATTA TATACATGTT ATGGGAAAAA GTTTGAACTT 6264 .......... .......... .......... .......... .......... .......... 412 TATGTTCATG TGCCTTTTAG AGGGTTAAAT AAGGCTTTGC TCCACTACTT ACTCAAACTG 6204 .......... .......... .......... .......... .......... .......... 412 GAATTGATTT ACTGAAAGTA TTAACTAGTG TAACTTTTCA AAAGAAAAGG GATTAACTAG 6144 .......... .......... .......... .......... .......... .......... 412 TTGGATGTTT GCGCTTAGAC TTTTATATGA ACAAATTCTT TTAAAATGCT GCCAGCCTAC 6084 .......... .......... .......... .......... .......... .......... 412 TATGTTTAAT ATTGAAACTT CTCTTTGTGT GTGCATACAG TGTTTATTGT GCAATACTCT 6024 .......... .......... .......... .......... .......... .......... 412 ACAAAATAGT ATTCAAGTTG TTGAGTGAGT TCTTACGGGA AGAGGTTTGC CTATCATTGC 5964 .......... .......... .......... .......... .......... .......... 412 CTCTAGAATG GGAATGTGGT TGAATGTTGC GTCGCTCATT CCCATGTGAT AAGTGAATGT 5904 .......... .......... .......... .......... .......... .......... 412 AATATGGTTC ACCGCAAATA AGTGGGGTGA GGAGAAATGT GATATGCGGC AATCATGAAC 5844 .......... .......... .......... .......... .......... .......... 412 CGGAATCTGG CATGTTTTGA CCATCACTTG AACAGAATCT ATAGTCAATA CTACCTTTCT 5784 .......... .......... .......... .......... .......... .......... 412 GGTTCAATTT CTGTTTTCCA GCATGGTGCT GCAATAATGA GATTCTGTTG TGTTTAGTTA 5724 .......... .......... .......... .......... .......... .......... 412 GCCAATATGT GGCTTTGCTG TTATCTCGTA CTAATTAATA CTACCAATTA ACACTGGATT 5664 .......... .......... .......... .......... .......... .......... 412 AAATATCTTA GTTTCACTCG GGGATTATGC TAACTTTCTC AAGTACAGGC ACAAGACGAG 5604 || |||||||||| .......... .......... .......... .......... ........GC ACAAGACGAG 424 GGTACATATT GGGCAGTTAG TGAAAGATAC ATCTGCAAAA CTTAAGCAAG CCAGCGAAAC 5544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTACATATT GGGCAGTTAG TGAAAGATAC ATCTGCAAAA CTTAAGCAAG CCAGCGAAAC 484 AGATCATCGT GTTGAAGTCA GTGTAAGTAT TAAGAGCTCA CCTATTTCTT CTTCTCCTTG 5484 |||||||||| |||||||||| || AGATCATCGT GTTGAAGTCA GT........ .......... .......... .......... 506 GTGTTTTCCT TTGTCAGTAT GGATTTCCAT GACATATATT TGCAGAATAC ACATGCTCGT 5424 .......... .......... .......... .......... .......... .......... 506 TTTCTTCTGT CCTCATTATA TATATGAGGT TCTCATCATT TTTGATTGAT TAGGCTTATG 5364 .......... .......... .......... .......... .......... .......... 506 TTTACTTCTT GTTCTTCACT CTTTAACTTT CTCTTTCACT ATTAACTATA AACTCCTGCT 5304 .......... .......... .......... .......... .......... .......... 506 TTCTTCTATC AGGCTAGCAA GAAAATAACA GATGCTAAAC TTGCTAAAGA TTTTCAAGCA 5244 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..GCTAGCAA GAAAATAACA GATGCTAAAC TTGCTAAAGA TTTTCAAGCA 554 GTCTTGAAGG AGTTTCAAAA GGCTCAAAGG CTTGCAGCTG AGAGAGAGAC AGCATATACA 5184 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTTGAAGG AGTTTCAAAA GGCTCAAAGG CTTGCAGCTG AGAGAGAGAC AGCATATACA 614 CCTTTTGTTC CCCAAGCAGT TCTTCCTTCA AGGTAAGTTT TCCTGATGGT ACTTTATCAT 5124 |||||||||| |||||||||| |||||||||| || CCTTTTGTTC CCCAAGCAGT TCTTCCTTCA AG........ .......... .......... 646 ATGAAATTTC TGTCAACTTT ATAAAAGTAT AAAAAGTATG AAATACATAT AAGCGATGTC 5064 .......... .......... .......... .......... .......... .......... 646 CCCTAGGGCT TTTATGTGAT GGTAAGAGTG CAACATGGGA TGTGTGGTCA AGCGCATGTT 5004 .......... .......... .......... .......... .......... .......... 646 GGGGTTCCTA GTATTTAAGT GGAGAACAGT AGAGGGGTGG GTGCATTATT CATGCAGTGT 4944 .......... .......... .......... .......... .......... .......... 646 CGGACCTTGT GCCGCATGTT CTCTTTCATG ATAATAAAGA AAATAATACA TATAGGCAAC 4884 .......... .......... .......... .......... .......... .......... 646 TTCTGCCTCA GACCGCAGGA AATGATAAAT TCATTCTCTC TAGTTTACAA TCTCAAGTGA 4824 .......... .......... .......... .......... .......... .......... 646 AATGTTTTGC CATCAATTTT ATTGATACCT CTCATTTTTG GGTATGGTCA TCTTTTGGTA 4764 .......... .......... .......... .......... .......... .......... 646 AACAGTTATA CAGCCAGTGA GATAGATGTT TCTTCGGGAA AGAGTCCAGA ACAGCGAGCT 4704 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....TTATA CAGCCAGTGA GATAGATGTT TCTTCGGGAA AGAGTCCAGA ACAGCGAGCT 701 CTCCTTGTGG AATCAAGAAG GTCAGTTAGC TGATTTCTTG TGAAGATTTA TTTAAATTTT 4644 |||||||||| |||||||||| CTCCTTGTGG AATCAAGAAG .......... .......... .......... .......... 721 GTGCTTATCT GTGAGATTGT TAATATCATA AATTATTAAA TTCAGCTATG CGTTAAGTTT 4584 .......... .......... .......... .......... .......... .......... 721 ATAGACACCA TGATGATTCG ATTAATTTGT TTGCTCTTTC CTTGTTGGCT TGTTCAAAGA 4524 .......... .......... .......... .......... .......... .......... 721 GTGTGCTTCT TAAAAGTAAC TTATATTTGT TGTGTTGCAG ACAAGAGGTT TTACTTTTGG 4464 |||||||||| |||||||||| .......... .......... .......... .......... ACAAGAGGTT TTACTTTTGG 741 ACAACGAGAT AGCCTTTAAT GAAGCAATCA TCGAAGAAAG AGAGCAGGGA ATACAAGAAA 4404 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACAACGAGAT AGCCTTTAAT GAAGCAATCA TCGAAGAAAG AGAGCAGGGA ATACAAGAAA 801 TACAACAACA AATTGGTGAA GTGAATGAAA TTTTCAAGGA CCTTGCTGTG CTTGTTCATG 4344 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACAACAACA AATTGGTGAA GTGAATGAAA TTTTCAAGGA CCTTGCTGTG CTTGTTCATG 861 AGCAAGGAGC TATGATTGGT AGGTCACTTT GTTTTGTTTT AGTATTCCAA ATTTCTATAT 4284 |||||||||| |||||||| AGCAAGGAGC TATGATTG.. .......... .......... .......... .......... 879 GGAATGTCCT TTGATATTTA CAGTTCTGTT TTCTGCAGAT GATATTGGTT CTAACGTTGA 4224 || |||||||||| |||||||||| .......... .......... .......... ........AT GATATTGGTT CTAACGTTGA 901 GAATGCTCAT GCTGCGACTG CACAGGGGAG ATCGCAACTT GCTAAAGCTG CAAAGACCCA 4164 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAATGCTCAT GCTGCGACTG CACAGGGGAG ATCGCAACTT GCTAAAGCTG CAAAGACCCA 961 AAGATCAAAT TCATCTCTGG TACTGTCTCA CATATGACCA TTCATCTTAC TGATCATAGA 4104 |||||||||| ||||||||| AAGATCAAAT TCATCTCTG. .......... .......... .......... .......... 980 ACTCTTATGA CTGGTATGAT TAAGCATGAT CGCTTTGGCT GAAGTCAAAG CTTTAATAGG 4044 .......... .......... .......... .......... .......... .......... 980 TTTATGATTC TGATACTGTG AAAAAACAGT AGGACCATTT AGAAGTTGGT GAGTTGTGCT 3984 .......... .......... .......... .......... .......... .......... 980 TTGCATTTGG GAATATTTTA GTTTATACCT TCTACATTTT AGAGTATGTT TGGTAGAGTA 3924 .......... .......... .......... .......... .......... .......... 980 GATGGAAGGT CCAAATTTGA TTAACATGCC GTCTTGACAA GTGTTCTCAC TGGACATATT 3864 .......... .......... .......... .......... .......... .......... 980 GACTGTGATG GTATGATAGG AGAGGGAAGT CATGAAACGA CTGGCAGTGG TAGGTTTCAT 3804 .......... .......... .......... .......... .......... .......... 980 CCTATTGGGA TACTTTAGAT GGTTCCTTTT TTAGATCATA CAGTCCATTC TATTTTTACA 3744 .......... .......... .......... .......... .......... .......... 980 TGGCCCTTAT ATCGAATCCG GTTGAGAAAC CAATCACATT GCGTATTTTC TTTCCCTTCT 3684 .......... .......... .......... .......... .......... .......... 980 GAGTACCACT TGTATTTTTT ATACTTACAT TCCTTACACC GAACAATATA TGCTCTGTAA 3624 .......... .......... .......... .......... .......... .......... 980 ATGATTTCCC TAACTTATAA CATTTTCTGC AGACTTGCTT ACTCCTGGTG ATTTTCGGAA 3564 |||||||| |||||||||| |||||||||| .......... .......... .......... ..ACTTGCTT ACTCCTGGTG ATTTTCGGAA 1008 TCGTGCTCCT CATTGTGATC GTAGTACTTG CAGCCTGAGC TGAAGATTAC TGGGATATAG 3504 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGTGCTCCT CATTGTGATC GTAGTACTTG CAGCCTGAGC TGAAGATTAC TGGGATATAG 1068 AGAAGGGTCC CGAACTCTGT TCAGACACAG AAGTGAGTGC TTTGATTCGA AAAAAAGTGA 3444 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAGGGTCC CGAACTCTGT TCAGACACAG AAGTGAGTGC TTTGATTCGA AAAAAAGTGA 1128 CTGTTATGAT TCGAGTCATT TCAATAGGCA TCCTGGAGTG TGGCTCGGTT GTCCCAACCC 3384 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTATGAT TCGAGTCATT TCAATAGGCA TCCTGGAGTG TGGCTCGGTT GTCCCAACCC 1188 TTGTTCTTCT TGTCATGGTT GATAAGTCGT GTAGTTAGGG AGGTGGGATA AGAAAGGTTC 3324 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTTCTTCT TGTCATGGTT GATAAGTCGT GTAGTTAGGG AGGTGGGATA AGAAAGGTTC 1248 CTGTTCAACT TGTAAACCTG GTGCTGTGTT TGCAAGATGT ATTGCTGACA TGTATTGGTG 3264 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTCAACT TGTAAACCTG GTGCTGTGTT TGCAAGATGT ATTGCTGACA TGTATTGGTG 1308 GGTCTGTCAA TAACAGTTTA TTTTTGTTTG AGGAGCAATC TCTTATATTT GCCCTTTGGA 3204 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTCTGTCAA TAACAGTTTA TTTTTGTTTG AGGAGCAATC TCTTATATTT GCCCTTTGGA 1368 TACTCTTGAA CCAGATGACA TTCAATTCTT TATTGATGCT TGTGTGTGTG TGAAGTTATC 3144 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACTCTTGAA CCAGATGACA TTCAATTCTT TATTGATGCT TGTGTGTGTG TGAAGTTATC 1428 ACTAATGGGA TATGTTGATT TGTTTATCTT GTCTGGTGGA TTATCTTGCA TG 3092 |||||||||| |||||||||| |||||| | ||| |||||||||| || ACTAATGGGA TATGTTGATT TGTTTAAAAA AAAAAGGGGA TTATCTTGCA TG 1480 hqPGS_C08HBa0217O07.1-_SGN-U315215+ (7343 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4145,3591 3092) ******************************************************************************** EST sequence 8 +strand 959 n (File: SGN-U315218+) 1 TTTTTGGCGC AATAATTATC TAATAAAATA CTCCTTCCTC ACATCTCCTT GCAAATTCCC 61 CCAACCCCAA ATTCTAGGGT TTCCATATTA CTTCTCTCTG TAATCCAAAT CGTTATCCGG 121 CTTTGTCCCC TCTGATCACA TACCTTTAGG TTTGGATTTC TGAAATTTGG GGTGAATTTT 181 GATCAGAATT CGAAGGAACA AGAGAAAAAA ATGAGCTTTC AAGATCTAGA ATCGGGTCGA 241 TCAGTGGGTC CACGTCGGGG CTTTATGAAC GGGAAGCAAG ACACTACCCA AGCGGTCGCC 301 TCAGGAATTT TCCAGATCAA CACCGCCGTC TCCACATTTC AGAGGCTGGT TAACACCCTA 361 GGTACACCTA AGGACACCCC TGAGCTACGC GACAAGCTGC ACAAGACAAG GCTACATATT 421 GGGCAATTAG TGAAAGATAC TTCCACAAAA CTTAAGCAAG CGAGTGAAAC AGATCATCGA 481 GTTGAAGTCA GTGCTAGCAA GAAAATTACA GATGCTAAAC TTGCTAAAGA CTTTCAAGCT 541 GTATTGAAGG AGTTTCAAAA AGCTCAACGG CTTGCAGCTG AGAGGGAAAC AGCATATACA 601 CCTTTTATTC CCCAAGCAGT TCTTCCTTCT AGTTACACAA ACAGTGAGGT AGATGTCTCT 661 TCAGATAAAA GTCAAGAACA GCGAGCTCTA CTTGTGGAAT CTAGAAGGCA AGAGGTTTTG 721 CTTTTGGACA ATGAGATTTC ATTTAATGAA GCTATTATAG AGGAAAGAGA CCAGGGAATA 781 CAAGAAGTAC AACAACAGAT AGGTGAAGTT AATGATATTT TCAAGGATCT TGCCGTGCTT 841 GTTCACGAGC AAGGAACCAT GATTGATGAT ATTGGTTCCA ACATTGAGAA CTCTCATGCT 901 GCAACTGCAC AAGGGAGATC ACAACTTGCT AAAGCTGCAA GACCCAAAGA TCAAATTCA Predicted gene structure (within gDNA segment 10946 to 1245): Exon 1 7192 6939 ( 254 n); cDNA 148 398 ( 251 n); score: 0.746 Intron 1 6938 5616 (1323 n); Pd: 0.983 (s: 0.72), Pa: 0.923 (s: 0.88) Exon 2 5615 5522 ( 94 n); cDNA 399 492 ( 94 n); score: 0.904 Intron 2 5521 5292 ( 230 n); Pd: 0.996 (s: 0.90), Pa: 0.999 (s: 0.94) Exon 3 5291 5152 ( 140 n); cDNA 493 632 ( 140 n); score: 0.929 Intron 3 5151 4759 ( 393 n); Pd: 1.000 (s: 0.92), Pa: 0.990 (s: 0.80) Exon 4 4758 4684 ( 75 n); cDNA 633 707 ( 75 n); score: 0.840 Intron 4 4683 4484 ( 200 n); Pd: 0.999 (s: 0.86), Pa: 0.998 (s: 0.84) Exon 5 4483 4326 ( 158 n); cDNA 708 865 ( 158 n); score: 0.867 Intron 5 4325 4246 ( 80 n); Pd: 0.996 (s: 0.88), Pa: 0.998 (s: 0.88) Exon 6 4245 4151 ( 95 n); cDNA 866 959 ( 94 n); score: 0.916 MATCH C08HBa0217O07.1- SGN-U315218+ 0.847 816 0.851 C PGS_C08HBa0217O07.1-_SGN-U315218+ (7192 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4151) Alignment (genomic DNA sequence = upper lines): AGGTTCCGAT TCCTGGATTT TGTGGGAGAA TTTTG-GGGG ATTCCAATTG TATATTAAGA 7134 ||||| ||| | ||| | || || ||| ||| ||||| | | | | | | | | |||| AGGTTTGGAT TTCTGAAATT TG-GGGTGAA TTTTGATCAG AATTCGA-AG GA-A-CAAGA 203 GAAAGAGATG AGTTTTCAAG ATCTTGAAGC GGGTCGACCA TTGGGGCCCC GTAGAGGGTA 7074 |||| | ||| || ||||||| |||| ||| | ||||||| || |||| || | || | || | GAAAAAAATG AGCTTTCAAG ATCTAGAATC GGGTCGATCA GTGGGTCCAC GTCGGGGCTT 263 TATGAATGGT AAGCAAGACC CAACTCAAGC TGTGGCTTCT GGAATTTTCC AGATTAACAC 7014 |||||| || ||||||||| | || ||||| || || || |||||||||| |||| ||||| TATGAACGGG AAGCAAGACA CTACCCAAGC GGTCGCCTCA GGAATTTTCC AGATCAACAC 323 TGCTGTGTCA ACGTTTCAAA GGCTGGTCAA TACACTTGGA ACACCCAAAG ATACACCCGA 6954 || || || || ||||| | ||||||| || || || || ||||| || | | || || || CGCCGTCTCC ACATTTCAGA GGCTGGTTAA CACCCTAGGT ACACCTAAGG ACACCCCTGA 383 GCTGCGTGAG AAGTTGTAAG CTCTCGAAAT TTTGTACGAT AAATTGAAGT TGTAAGCTGA 6894 ||| || || ||| | GCTACGCGAC AAGCT..... .......... .......... .......... .......... 398 ATTTTTGGAT GAAATTTTGC AATTTTTAAT ACAGTTGGAA TTGCTTACAT TTCTTGTTGA 6834 .......... .......... .......... .......... .......... .......... 398 ATTTGATGTT TTGTGGACTT ACTGTATTGT CAATTGATGG ATTAATTCTG AGACAAGTTT 6774 .......... .......... .......... .......... .......... .......... 398 ATAGCTTTTG TGGTGATTGA GGTCGCACTT TAGCCATAAT CATTTATTGA GATAGACCGG 6714 .......... .......... .......... .......... .......... .......... 398 GTCAACTGAT ACTGTTTTGT GGTATTTTTG TTAGGCGATT TCATCTTTTA CTGTGAATTC 6654 .......... .......... .......... .......... .......... .......... 398 GTGAATTTAT AACTCTTCCT GTATTAGTTT TAGCGTACAC ACAGAGAGTC AGAAAGAGCA 6594 .......... .......... .......... .......... .......... .......... 398 TTATACTTGA AACTGATGTT CTGCATTTGA GGAAACTCTT TAGGAAGGCC TCAGCACATG 6534 .......... .......... .......... .......... .......... .......... 398 GGCACACACT AAAAATGATG TCTAAATTAT CACTGAACAT GTGCAAATGA GTTCTTCTGA 6474 .......... .......... .......... .......... .......... .......... 398 TCACTTTGAG AGTTTTGATA TAGAGAAAAT CAGTAAGCTT GCAAAAATAT TATAAATCAA 6414 .......... .......... .......... .......... .......... .......... 398 AATTTTAGAA AACCATTTTC AGTTTTTTTG TCCACCTGTA CTTCGTGTGG ATGACTGGGA 6354 .......... .......... .......... .......... .......... .......... 398 AGTATATATT GTTCAGTATA TGGTTCTGAT GAATGATAAA ACATGTTTTT AGGCTAATTA 6294 .......... .......... .......... .......... .......... .......... 398 TATACATGTT ATGGGAAAAA GTTTGAACTT TATGTTCATG TGCCTTTTAG AGGGTTAAAT 6234 .......... .......... .......... .......... .......... .......... 398 AAGGCTTTGC TCCACTACTT ACTCAAACTG GAATTGATTT ACTGAAAGTA TTAACTAGTG 6174 .......... .......... .......... .......... .......... .......... 398 TAACTTTTCA AAAGAAAAGG GATTAACTAG TTGGATGTTT GCGCTTAGAC TTTTATATGA 6114 .......... .......... .......... .......... .......... .......... 398 ACAAATTCTT TTAAAATGCT GCCAGCCTAC TATGTTTAAT ATTGAAACTT CTCTTTGTGT 6054 .......... .......... .......... .......... .......... .......... 398 GTGCATACAG TGTTTATTGT GCAATACTCT ACAAAATAGT ATTCAAGTTG TTGAGTGAGT 5994 .......... .......... .......... .......... .......... .......... 398 TCTTACGGGA AGAGGTTTGC CTATCATTGC CTCTAGAATG GGAATGTGGT TGAATGTTGC 5934 .......... .......... .......... .......... .......... .......... 398 GTCGCTCATT CCCATGTGAT AAGTGAATGT AATATGGTTC ACCGCAAATA AGTGGGGTGA 5874 .......... .......... .......... .......... .......... .......... 398 GGAGAAATGT GATATGCGGC AATCATGAAC CGGAATCTGG CATGTTTTGA CCATCACTTG 5814 .......... .......... .......... .......... .......... .......... 398 AACAGAATCT ATAGTCAATA CTACCTTTCT GGTTCAATTT CTGTTTTCCA GCATGGTGCT 5754 .......... .......... .......... .......... .......... .......... 398 GCAATAATGA GATTCTGTTG TGTTTAGTTA GCCAATATGT GGCTTTGCTG TTATCTCGTA 5694 .......... .......... .......... .......... .......... .......... 398 CTAATTAATA CTACCAATTA ACACTGGATT AAATATCTTA GTTTCACTCG GGGATTATGC 5634 .......... .......... .......... .......... .......... .......... 398 TAACTTTCTC AAGTACAGGC ACAAGACGAG GGTACATATT GGGCAGTTAG TGAAAGATAC 5574 || ||||||| || | |||||||| ||||| |||| |||||||||| .......... ........GC ACAAGACAAG GCTACATATT GGGCAATTAG TGAAAGATAC 440 ATCTGCAAAA CTTAAGCAAG CCAGCGAAAC AGATCATCGT GTTGAAGTCA GTGTAAGTAT 5514 || ||||| |||||||||| | || ||||| ||||||||| |||||||||| || TTCCACAAAA CTTAAGCAAG CGAGTGAAAC AGATCATCGA GTTGAAGTCA GT........ 492 TAAGAGCTCA CCTATTTCTT CTTCTCCTTG GTGTTTTCCT TTGTCAGTAT GGATTTCCAT 5454 .......... .......... .......... .......... .......... .......... 492 GACATATATT TGCAGAATAC ACATGCTCGT TTTCTTCTGT CCTCATTATA TATATGAGGT 5394 .......... .......... .......... .......... .......... .......... 492 TCTCATCATT TTTGATTGAT TAGGCTTATG TTTACTTCTT GTTCTTCACT CTTTAACTTT 5334 .......... .......... .......... .......... .......... .......... 492 CTCTTTCACT ATTAACTATA AACTCCTGCT TTCTTCTATC AGGCTAGCAA GAAAATAACA 5274 |||||||| |||||| ||| .......... .......... .......... .......... ..GCTAGCAA GAAAATTACA 510 GATGCTAAAC TTGCTAAAGA TTTTCAAGCA GTCTTGAAGG AGTTTCAAAA GGCTCAAAGG 5214 |||||||||| |||||||||| |||||||| || ||||||| |||||||||| |||||| || GATGCTAAAC TTGCTAAAGA CTTTCAAGCT GTATTGAAGG AGTTTCAAAA AGCTCAACGG 570 CTTGCAGCTG AGAGAGAGAC AGCATATACA CCTTTTGTTC CCCAAGCAGT TCTTCCTTCA 5154 |||||||||| |||| || || |||||||||| |||||| ||| |||||||||| ||||||||| CTTGCAGCTG AGAGGGAAAC AGCATATACA CCTTTTATTC CCCAAGCAGT TCTTCCTTCT 630 AGGTAAGTTT TCCTGATGGT ACTTTATCAT ATGAAATTTC TGTCAACTTT ATAAAAGTAT 5094 || AG........ .......... .......... .......... .......... .......... 632 AAAAAGTATG AAATACATAT AAGCGATGTC CCCTAGGGCT TTTATGTGAT GGTAAGAGTG 5034 .......... .......... .......... .......... .......... .......... 632 CAACATGGGA TGTGTGGTCA AGCGCATGTT GGGGTTCCTA GTATTTAAGT GGAGAACAGT 4974 .......... .......... .......... .......... .......... .......... 632 AGAGGGGTGG GTGCATTATT CATGCAGTGT CGGACCTTGT GCCGCATGTT CTCTTTCATG 4914 .......... .......... .......... .......... .......... .......... 632 ATAATAAAGA AAATAATACA TATAGGCAAC TTCTGCCTCA GACCGCAGGA AATGATAAAT 4854 .......... .......... .......... .......... .......... .......... 632 TCATTCTCTC TAGTTTACAA TCTCAAGTGA AATGTTTTGC CATCAATTTT ATTGATACCT 4794 .......... .......... .......... .......... .......... .......... 632 CTCATTTTTG GGTATGGTCA TCTTTTGGTA AACAGTTATA CAGCCAGTGA GATAGATGTT 4734 ||| | || |||||| | ||||||| .......... .......... .......... .....TTACA CAAACAGTGA GGTAGATGTC 657 TCTTCGGGAA AGAGTCCAGA ACAGCGAGCT CTCCTTGTGG AATCAAGAAG GTCAGTTAGC 4674 ||||| | | | |||| ||| |||||||||| || ||||||| |||| ||||| TCTTCAGATA AAAGTCAAGA ACAGCGAGCT CTACTTGTGG AATCTAGAAG .......... 707 TGATTTCTTG TGAAGATTTA TTTAAATTTT GTGCTTATCT GTGAGATTGT TAATATCATA 4614 .......... .......... .......... .......... .......... .......... 707 AATTATTAAA TTCAGCTATG CGTTAAGTTT ATAGACACCA TGATGATTCG ATTAATTTGT 4554 .......... .......... .......... .......... .......... .......... 707 TTGCTCTTTC CTTGTTGGCT TGTTCAAAGA GTGTGCTTCT TAAAAGTAAC TTATATTTGT 4494 .......... .......... .......... .......... .......... .......... 707 TGTGTTGCAG ACAAGAGGTT TTACTTTTGG ACAACGAGAT AGCCTTTAAT GAAGCAATCA 4434 ||||||||| || ||||||| |||| ||||| | |||||| ||||| || | .......... GCAAGAGGTT TTGCTTTTGG ACAATGAGAT TTCATTTAAT GAAGCTATTA 757 TCGAAGAAAG AGAGCAGGGA ATACAAGAAA TACAACAACA AATTGGTGAA GTGAATGAAA 4374 | || ||||| ||| |||||| ||||||||| |||||||||| || |||||| || ||||| | TAGAGGAAAG AGACCAGGGA ATACAAGAAG TACAACAACA GATAGGTGAA GTTAATGATA 817 TTTTCAAGGA CCTTGCTGTG CTTGTTCATG AGCAAGGAGC TATGATTGGT AGGTCACTTT 4314 |||||||||| ||||| ||| |||||||| | |||||||| | ||||||| TTTTCAAGGA TCTTGCCGTG CTTGTTCACG AGCAAGGAAC CATGATTG.. .......... 865 GTTTTGTTTT AGTATTCCAA ATTTCTATAT GGAATGTCCT TTGATATTTA CAGTTCTGTT 4254 .......... .......... .......... .......... .......... .......... 865 TTCTGCAGAT GATATTGGTT CTAACGTTGA GAATGCTCAT GCTGCGACTG CACAGGGGAG 4194 || |||||||||| | ||| |||| ||| ||||| ||||| |||| |||| ||||| ........AT GATATTGGTT CCAACATTGA GAACTCTCAT GCTGCAACTG CACAAGGGAG 917 ATCGCAACTT GCTAAAGCTG CAAAGACCCA AAGATCAAAT TCA 4151 ||| |||||| |||||||||| | |||||||| |||||||||| ||| ATCACAACTT GCTAAAGCTG C-AAGACCCA AAGATCAAAT TCA 959 hqPGS_C08HBa0217O07.1-_SGN-U315218+ (7192 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4151) ******************************************************************************** EST sequence 6 +strand 525 n (File: SGN-U315216+) 1 GAATTTAGGT TTTGGATTTC TGAATTTTGA TCAAAATTCG AAGAAATGAG CTTTCAAGAT 61 CTAGATTCCG GTCGATCATC GGGTCCTCGT CGGAGCAATG TCAACGGGAA GCAAGACGCC 121 ACTCAAGCTT TGGCCTCTGG AATTTTCCAG ATCAACACCG CCGTTTCCAC TTTTCAGAGA 181 CTGGTTAATA CCCTAGGTAC CCCTAAGGAT ACCCCGGAGC TACGTGACAA GTTGCACAAG 241 ACAAGACTAC ATATTGGGCA ATTAGTGAAA GATACTTCTG CTAAACTTAA GCAAGCCAGC 301 GAAACAGATC ATCGAATTGA AGTCAGTGCT AGCAAGAAAA TTACAGATGC GAAGCTTGCT 361 AAAGATTTTC AAGCTGTATT GAAGGAGTTT CAAAAAGCTC AGAGGCTTGC TGCTGAGAGG 421 GAAACGTCAT ATTCACCTTT TGTTCCCCAA GCAGTTCTTC CTTCTAGTTA CACAAACAGT 481 GAGATAGATG TCTCTTCAGA TAAAAGTCAA GAACAGCGAG CTCTA Predicted gene structure (within gDNA segment 8986 to 3935): Exon 1 7126 6939 ( 188 n); cDNA 46 233 ( 188 n); score: 0.782 Intron 1 6938 5616 (1323 n); Pd: 0.983 (s: 0.78), Pa: 0.923 (s: 0.88) Exon 2 5615 5522 ( 94 n); cDNA 234 327 ( 94 n); score: 0.915 Intron 2 5521 5292 ( 230 n); Pd: 0.996 (s: 0.94), Pa: 0.999 (s: 0.92) Exon 3 5291 5152 ( 140 n); cDNA 328 467 ( 140 n); score: 0.900 Intron 3 5151 4759 ( 393 n); Pd: 1.000 (s: 0.88), Pa: 0.990 (s: 0.82) Exon 4 4758 4702 ( 57 n); cDNA 468 524 ( 57 n); score: 0.842 MATCH C08HBa0217O07.1- SGN-U315216+ 0.850 479 0.912 C PGS_C08HBa0217O07.1-_SGN-U315216+ (7126 6939,5615 5522,5291 5152,4758 4702) Alignment (genomic DNA sequence = upper lines): ATGAGTTTTC AAGATCTTGA AGCGGGTCGA CCATTGGGGC CCCGTAGAGG GTATATGAAT 7067 ||||| |||| ||||||| || | |||||| ||| ||| | | ||| | | || | || ATGAGCTTTC AAGATCTAGA TTCCGGTCGA TCATCGGGTC CTCGTCGGAG CAATGTCAAC 105 GGTAAGCAAG ACCCAACTCA AGCTGTGGCT TCTGGAATTT TCCAGATTAA CACTGCTGTG 7007 || ||||||| || | ||||| |||| |||| |||||||||| ||||||| || ||| || || GGGAAGCAAG ACGCCACTCA AGCTTTGGCC TCTGGAATTT TCCAGATCAA CACCGCCGTT 165 TCAACGTTTC AAAGGCTGGT CAATACACTT GGAACACCCA AAGATACACC CGAGCTGCGT 6947 || || |||| | || ||||| ||||| || || || || | | ||||| || ||||| ||| TCCACTTTTC AGAGACTGGT TAATACCCTA GGTACCCCTA AGGATACCCC GGAGCTACGT 225 GAGAAGTTGT AAGCTCTCGA AATTTTGTAC GATAAATTGA AGTTGTAAGC TGAATTTTTG 6887 || ||||| GACAAGTT.. .......... .......... .......... .......... .......... 233 GATGAAATTT TGCAATTTTT AATACAGTTG GAATTGCTTA CATTTCTTGT TGAATTTGAT 6827 .......... .......... .......... .......... .......... .......... 233 GTTTTGTGGA CTTACTGTAT TGTCAATTGA TGGATTAATT CTGAGACAAG TTTATAGCTT 6767 .......... .......... .......... .......... .......... .......... 233 TTGTGGTGAT TGAGGTCGCA CTTTAGCCAT AATCATTTAT TGAGATAGAC CGGGTCAACT 6707 .......... .......... .......... .......... .......... .......... 233 GATACTGTTT TGTGGTATTT TTGTTAGGCG ATTTCATCTT TTACTGTGAA TTCGTGAATT 6647 .......... .......... .......... .......... .......... .......... 233 TATAACTCTT CCTGTATTAG TTTTAGCGTA CACACAGAGA GTCAGAAAGA GCATTATACT 6587 .......... .......... .......... .......... .......... .......... 233 TGAAACTGAT GTTCTGCATT TGAGGAAACT CTTTAGGAAG GCCTCAGCAC ATGGGCACAC 6527 .......... .......... .......... .......... .......... .......... 233 ACTAAAAATG ATGTCTAAAT TATCACTGAA CATGTGCAAA TGAGTTCTTC TGATCACTTT 6467 .......... .......... .......... .......... .......... .......... 233 GAGAGTTTTG ATATAGAGAA AATCAGTAAG CTTGCAAAAA TATTATAAAT CAAAATTTTA 6407 .......... .......... .......... .......... .......... .......... 233 GAAAACCATT TTCAGTTTTT TTGTCCACCT GTACTTCGTG TGGATGACTG GGAAGTATAT 6347 .......... .......... .......... .......... .......... .......... 233 ATTGTTCAGT ATATGGTTCT GATGAATGAT AAAACATGTT TTTAGGCTAA TTATATACAT 6287 .......... .......... .......... .......... .......... .......... 233 GTTATGGGAA AAAGTTTGAA CTTTATGTTC ATGTGCCTTT TAGAGGGTTA AATAAGGCTT 6227 .......... .......... .......... .......... .......... .......... 233 TGCTCCACTA CTTACTCAAA CTGGAATTGA TTTACTGAAA GTATTAACTA GTGTAACTTT 6167 .......... .......... .......... .......... .......... .......... 233 TCAAAAGAAA AGGGATTAAC TAGTTGGATG TTTGCGCTTA GACTTTTATA TGAACAAATT 6107 .......... .......... .......... .......... .......... .......... 233 CTTTTAAAAT GCTGCCAGCC TACTATGTTT AATATTGAAA CTTCTCTTTG TGTGTGCATA 6047 .......... .......... .......... .......... .......... .......... 233 CAGTGTTTAT TGTGCAATAC TCTACAAAAT AGTATTCAAG TTGTTGAGTG AGTTCTTACG 5987 .......... .......... .......... .......... .......... .......... 233 GGAAGAGGTT TGCCTATCAT TGCCTCTAGA ATGGGAATGT GGTTGAATGT TGCGTCGCTC 5927 .......... .......... .......... .......... .......... .......... 233 ATTCCCATGT GATAAGTGAA TGTAATATGG TTCACCGCAA ATAAGTGGGG TGAGGAGAAA 5867 .......... .......... .......... .......... .......... .......... 233 TGTGATATGC GGCAATCATG AACCGGAATC TGGCATGTTT TGACCATCAC TTGAACAGAA 5807 .......... .......... .......... .......... .......... .......... 233 TCTATAGTCA ATACTACCTT TCTGGTTCAA TTTCTGTTTT CCAGCATGGT GCTGCAATAA 5747 .......... .......... .......... .......... .......... .......... 233 TGAGATTCTG TTGTGTTTAG TTAGCCAATA TGTGGCTTTG CTGTTATCTC GTACTAATTA 5687 .......... .......... .......... .......... .......... .......... 233 ATACTACCAA TTAACACTGG ATTAAATATC TTAGTTTCAC TCGGGGATTA TGCTAACTTT 5627 .......... .......... .......... .......... .......... .......... 233 CTCAAGTACA GGCACAAGAC GAGGGTACAT ATTGGGCAGT TAGTGAAAGA TACATCTGCA 5567 ||||||||| || ||||| |||||||| | |||||||||| ||| ||||| .......... .GCACAAGAC AAGACTACAT ATTGGGCAAT TAGTGAAAGA TACTTCTGCT 282 AAACTTAAGC AAGCCAGCGA AACAGATCAT CGTGTTGAAG TCAGTGTAAG TATTAAGAGC 5507 |||||||||| |||||||||| |||||||||| || |||||| ||||| AAACTTAAGC AAGCCAGCGA AACAGATCAT CGAATTGAAG TCAGT..... .......... 327 TCACCTATTT CTTCTTCTCC TTGGTGTTTT CCTTTGTCAG TATGGATTTC CATGACATAT 5447 .......... .......... .......... .......... .......... .......... 327 ATTTGCAGAA TACACATGCT CGTTTTCTTC TGTCCTCATT ATATATATGA GGTTCTCATC 5387 .......... .......... .......... .......... .......... .......... 327 ATTTTTGATT GATTAGGCTT ATGTTTACTT CTTGTTCTTC ACTCTTTAAC TTTCTCTTTC 5327 .......... .......... .......... .......... .......... .......... 327 ACTATTAACT ATAAACTCCT GCTTTCTTCT ATCAGGCTAG CAAGAAAATA ACAGATGCTA 5267 ||||| ||||||||| |||||||| | .......... .......... .......... .....GCTAG CAAGAAAATT ACAGATGCGA 352 AACTTGCTAA AGATTTTCAA GCAGTCTTGA AGGAGTTTCA AAAGGCTCAA AGGCTTGCAG 5207 | |||||||| |||||||||| || || |||| |||||||||| ||| ||||| |||||||| | AGCTTGCTAA AGATTTTCAA GCTGTATTGA AGGAGTTTCA AAAAGCTCAG AGGCTTGCTG 412 CTGAGAGAGA GACAGCATAT ACACCTTTTG TTCCCCAAGC AGTTCTTCCT TCAAGGTAAG 5147 ||||||| || || ||||| ||||||||| |||||||||| |||||||||| || || CTGAGAGGGA AACGTCATAT TCACCTTTTG TTCCCCAAGC AGTTCTTCCT TCTAG..... 467 TTTTCCTGAT GGTACTTTAT CATATGAAAT TTCTGTCAAC TTTATAAAAG TATAAAAAGT 5087 .......... .......... .......... .......... .......... .......... 467 ATGAAATACA TATAAGCGAT GTCCCCTAGG GCTTTTATGT GATGGTAAGA GTGCAACATG 5027 .......... .......... .......... .......... .......... .......... 467 GGATGTGTGG TCAAGCGCAT GTTGGGGTTC CTAGTATTTA AGTGGAGAAC AGTAGAGGGG 4967 .......... .......... .......... .......... .......... .......... 467 TGGGTGCATT ATTCATGCAG TGTCGGACCT TGTGCCGCAT GTTCTCTTTC ATGATAATAA 4907 .......... .......... .......... .......... .......... .......... 467 AGAAAATAAT ACATATAGGC AACTTCTGCC TCAGACCGCA GGAAATGATA AATTCATTCT 4847 .......... .......... .......... .......... .......... .......... 467 CTCTAGTTTA CAATCTCAAG TGAAATGTTT TGCCATCAAT TTTATTGATA CCTCTCATTT 4787 .......... .......... .......... .......... .......... .......... 467 TTGGGTATGG TCATCTTTTG GTAAACAGTT ATACAGCCAG TGAGATAGAT GTTTCTTCGG 4727 || | ||| ||| |||||||||| || ||||| | .......... .......... ........TT ACACAAACAG TGAGATAGAT GTCTCTTCAG 499 GAAAGAGTCC AGAACAGCGA GCTCT 4702 || |||| |||||||||| ||||| ATAAAAGTCA AGAACAGCGA GCTCT 524 hqPGS_C08HBa0217O07.1-_SGN-U315216+ (7126 6939,5615 5522,5291 5152,4758 4702) ******************************************************************************** EST sequence 7 +strand 591 n (File: SGN-U315217+) 1 AGATTTTTGG TTTTTGGGAA TTTCACCTGA AAATTTTGGA CTTCTCAAAG TTCCCTCAAT 61 TTCCTTACAA ATTCTCCAAA CCCCATTATT CTTATTGTGA TCAAGATTTG ATTTTATTTA 121 TTTCTGTGTA TATAACTGTA GATTTTAATT GTTGGGTAGT TGAGATTTTT CGAAGAAATA 181 GAACAAAAGT ATGAGTTTTC AGGATTTGGA ATCGGGTCGA CCTTTGGGGT CTCGTCGGTT 241 CCAGACAAAT GGGAAGCAAG ACCCGACCCA AGCAGTGGCT TCTGGAATCT TCCAGATCAA 301 TACTGCTGTG TCTACGTTTC AGAGGCTAGT GAATACACTT GGAACCCCAA AAGATACACC 361 TGAGCTACGT GATAAGTTGC ACAAGACAAG GGTTCATATT GGCCAGTTAG TCAAAGATAC 421 ATCTGCAAAA CTTAAGCAAG CAAGTGAAAC AGATCATCAT GCTGATGTTA GTGCTAGCAA 481 GAAAATAACA GATGCTAAAC TTGCTAAAGA TTTTCAAGCT GTCTTAAAAG AGTTTCAAAA 541 GGCTCAGAGA CTTGCAGCAG AGAGGGAGAC AGCATATACA CCTTTCATTC C Predicted gene structure (within gDNA segment 11811 to 4158): Exon 1 11204 11156 ( 49 n); cDNA 137 184 ( 48 n); score: 0.653 Intron 1 11155 7133 (4023 n); Pd: 0.000 (s: 0.65), Pa: 0.000 (s: 0.80) Exon 2 7132 6939 ( 194 n); cDNA 185 378 ( 194 n); score: 0.820 Intron 2 6938 5616 (1323 n); Pd: 0.983 (s: 0.88), Pa: 0.923 (s: 0.92) Exon 3 5615 5522 ( 94 n); cDNA 379 472 ( 94 n); score: 0.894 Intron 3 5521 5292 ( 230 n); Pd: 0.996 (s: 0.88), Pa: 0.999 (s: 0.98) Exon 4 5291 5173 ( 119 n); cDNA 473 591 ( 119 n); score: 0.924 MATCH C08HBa0217O07.1- SGN-U315217+ 0.867 456 0.772 C PGS_C08HBa0217O07.1-_SGN-U315217+ (11204 11156,7132 6939,5615 5522,5291 5173) Alignment (genomic DNA sequence = upper lines): TGAAGTTTTG AACTCTTCAA TTAATTGAGA ATTATTCAAG TGATATAACA TTATTGAATA 11145 || || ||| || | || | | |||||| || | ||| ||| ||| TGTAGATTTT AATTGTTGGG T-AGTTGAGA TTTTTCGAAG AAATAGAAC. .......... 184 ATAATTGAAT CTGGATAGAT CAATATATAT TCATACTCTC ACATGTCTTA TAGAAAAATT 11085 .......... .......... .......... .......... .......... .......... 184 TTACGAAGGT TGTCCAAGTA TTACATAAGC AATTTTTTTC GATGAATGCA TTTTGAAAAA 11025 .......... .......... .......... .......... .......... .......... 184 ATTAAATTTG AACTGTGTAA TTTTAATATT ACCTTTTTTA TGAATAAATA TTTCAAATTT 10965 .......... .......... .......... .......... .......... .......... 184 TTAAAAATCA AATTGCATAA TTTTTAGCTT TAATATTTGT ATTTAATTTA ATATAAAGTG 10905 .......... .......... .......... .......... .......... .......... 184 AACAAACAAA CAAAAAATAA GGGGTGGGAG GGGGGAGGGG GGGAGGAATT AAGCTTAAAA 10845 .......... .......... .......... .......... .......... .......... 184 TTAAATCATT TTTATATTTC AAGCTTTCAC TAATCTATGA TGATCACAAC AATAACGAGA 10785 .......... .......... .......... .......... .......... .......... 184 CATGAATAAT GGAAGCAAAA TCAAACATGT GTTTTTGTGT GAGTTTTTGA CTAAATTCAT 10725 .......... .......... .......... .......... .......... .......... 184 CTCTCAGTTA TGACTGGAAT TTAATGTACA TCTTTATATT ATCTAAGTGA GAAAAAACAT 10665 .......... .......... .......... .......... .......... .......... 184 TTTCATACTA TTCAAAAAAT AACAAGAATC ATCCTTCAGT CTATTTTTGT TAAGAAACTA 10605 .......... .......... .......... .......... .......... .......... 184 ATTGAACAAA TAAAAAAGAA TATTTTTTTT AATTTGATTT TGTCTTAGCT ACATCGAGAA 10545 .......... .......... .......... .......... .......... .......... 184 GATCAATATT TCCCAAAAAG AACAAGGATA TGGATATAAA ATAAATATTT TATTCTCATA 10485 .......... .......... .......... .......... .......... .......... 184 ATTTAAATTT TGTGTGATGT TAGTTTATAT TTTTATTTTT TTTGCATAAT CAAGTAAATT 10425 .......... .......... .......... .......... .......... .......... 184 GACTTGCAAC AATACATAAG TTTTGAAATA TATATACAGT AGGATCCAAT TGTTTGAAAG 10365 .......... .......... .......... .......... .......... .......... 184 ATTATGCAAA AAAAAAAAAA TATTTAAAAA ATATTATGTG CAACTATTTA GAGATTCTCT 10305 .......... .......... .......... .......... .......... .......... 184 TTAATAAAAA TATATATATA GTACATATAT ACTATATCGA CAATGCAATT CACATTTTAT 10245 .......... .......... .......... .......... .......... .......... 184 ACAATATATA AAAATACCGG TAATTTAGTG AAATTATGTA GGCATTTTGT TGTGAAAATT 10185 .......... .......... .......... .......... .......... .......... 184 AAATAATAAT TACTTTATTT GAAATTTTTG AAAATGTAGT TGTATTTGGA CAATTTTTTT 10125 .......... .......... .......... .......... .......... .......... 184 TACAAATAAT ATTAAGAATT TGAATATATT TTGTCTGAAT ATTTTAATTC TCAATTTCTA 10065 .......... .......... .......... .......... .......... .......... 184 AAAATAAAAA AAAAGTCACC CAACTTGACT AACTTAATGA ACGTACGTAC ATATAATCAT 10005 .......... .......... .......... .......... .......... .......... 184 TCGACTTGAC ACATTTTTTG TTGATGGCCT GAGTTTCTTA AAAAGAAAAT AGACACAAGA 9945 .......... .......... .......... .......... .......... .......... 184 ATGAATCTTG CAACTTTTTG AATAGATCAA CGATGTCTTT TGTTCATTTT TATAAATACA 9885 .......... .......... .......... .......... .......... .......... 184 AGGATGTACT TTAGATTTAA GTTACAGTTG AGGGATGATT TTAGCCAAAA ACTCATTTTT 9825 .......... .......... .......... .......... .......... .......... 184 TTTCTTTTAG TAGCTTTATA GAAATAATTT TTATTAAATT AAATAATTAC ATTTAAGTTT 9765 .......... .......... .......... .......... .......... .......... 184 AATTTCAAAT TTTAATTAAA AAATAAAAAA TCAAATTCAT TGAAAATTCA ATAACCTACT 9705 .......... .......... .......... .......... .......... .......... 184 AAACCTAGAT TTCTAATTAA GAATGTGATT AATTAGCTTA GACAATCAGA GAAAAAAGTT 9645 .......... .......... .......... .......... .......... .......... 184 AACAACTTAG AGTAATAATA AAATAGGAGT GTTCTATTAA AAATGATTGT AAAGAGCAGC 9585 .......... .......... .......... .......... .......... .......... 184 TATGAGGAAT CAAACTCACG CCTACAAGCT ACAATGTAGG TAAGGAAAGC CTCACAGTGC 9525 .......... .......... .......... .......... .......... .......... 184 TAAGCCGCAA CATTATATTC ATAATGTCCA ACATGTTAAA TTTAACAGTT ACAAATATTT 9465 .......... .......... .......... .......... .......... .......... 184 TTTTTACTTA TATATATACA ATTAATTTTT CGATAAAAAG CATCTAATTA GACACCCTTA 9405 .......... .......... .......... .......... .......... .......... 184 AAACACTGTA CTCAACACTA TTTTGTCAAT TCCAAACAAA AAGAGAGCTT CTATTGTAGT 9345 .......... .......... .......... .......... .......... .......... 184 AGCAGTAGCT TCTTATTTTG TCATTCTGAT ACTCTTTTAA GTCTTGTTCA TGGTCCTTGG 9285 .......... .......... .......... .......... .......... .......... 184 ATATATAATG TAGCTTCGTG TGATGTTTCT CTATACTCAA CACTATTGTG TACTCCATTA 9225 .......... .......... .......... .......... .......... .......... 184 TTTTTAGTGG AGTCAATTTG AAGCTATCCT TTTATATTAA AGATGAACGT TCTTAAATAG 9165 .......... .......... .......... .......... .......... .......... 184 AGTTCTTTTT TATCATTATT ATTTATATGT CAAAGGAGAG GTAACAGAAT AACAATAATA 9105 .......... .......... .......... .......... .......... .......... 184 TTAGTGTGAT TTTATCAAGT ATTTTTTTTT AAAAAAATGA ATTTTAAGTA TTATGCAAAT 9045 .......... .......... .......... .......... .......... .......... 184 ATTAATCTTC TCTCCGAAGT ATAAAAGTTA TTTCTAATAA ATGATTTGTA ATTCAAAGAA 8985 .......... .......... .......... .......... .......... .......... 184 AAACATTTTA AAGCAGTACA TAAAAAGAAT AATGGAAGCA CAATAAAAAA TATATATAAT 8925 .......... .......... .......... .......... .......... .......... 184 AATTTTTTTA AATAAAAATT ACAACAAAAT AATACATTCA AAGCTCAAAG TAATAGAAAT 8865 .......... .......... .......... .......... .......... .......... 184 CAAAAGATAA AAAAATTACA AAAACAATAT TAAACTAGTA ATAAGGATGA ATATCAACAC 8805 .......... .......... .......... .......... .......... .......... 184 AATACATTGC TACCTAGTAA CTTTCTTCGT TTCTTTCGTA ACCTCTTATC CAAATTTTAA 8745 .......... .......... .......... .......... .......... .......... 184 GTAAATAAAA AATATATTGT ATCATTTTAA TCATCTTTTT CTAATGCTTC TTCAATTGAT 8685 .......... .......... .......... .......... .......... .......... 184 CTTTGCCTCT CTCAAATTGT CTATAGTCAA TCTCTCAATC CGTCTTTATA TATTTGAATG 8625 .......... .......... .......... .......... .......... .......... 184 ATCTCAATTT CATTTTTCAT ATTTTATCCT TTTCTAATAT CTTCATTTCT AATTTATATA 8565 .......... .......... .......... .......... .......... .......... 184 CTTTCTCCGT TTAAAAAGTA ATGATCTCTT TCCCTTTTTT TAGTTAGTTT AAAAAAGATT 8505 .......... .......... .......... .......... .......... .......... 184 GATCTTTCTT TTTTTTTGGT AACAATTTTT CACATGACAT GTTTAAAATC AGTATATTAA 8445 .......... .......... .......... .......... .......... .......... 184 AAGACAATTT TATACATTTG AAATAACTTT AAATATGATC AAAAGTTTTC TTTATTTTTT 8385 .......... .......... .......... .......... .......... .......... 184 AAAATTACAT GTCAAATTAA ATTAGATCTC CGTGAAACGA AGAAAGTATT ATGAGATGGA 8325 .......... .......... .......... .......... .......... .......... 184 ATACCTACAC ATCCATCCCG ACATTTCATT ACGGCGATGG AATAATTCTA TAATTCTGTT 8265 .......... .......... .......... .......... .......... .......... 184 GCGTCGTGCA TCACTGCTTA TTTGATTACA CTAGAAAATG TGTTGCATGT CTTATAAACA 8205 .......... .......... .......... .......... .......... .......... 184 ATTCTAATAT GAACAGCTAA ATATACAAAG TACATACGCA TAAAGCTGCC GACAATATCC 8145 .......... .......... .......... .......... .......... .......... 184 ACCTGTCACA TTATATAACC ACACCAGTCT AGCCGGCCAT TACATGGCGC TGATTTATTA 8085 .......... .......... .......... .......... .......... .......... 184 CTATTTTATT CAAAAATATA TCCCGCTAAA CCTAACCCCC ACCACCCAAC GCATATTTAA 8025 .......... .......... .......... .......... .......... .......... 184 TCATTGTGTC CCTTTCCCAC TATTTATTTT CTTCCATATT ATAAAATAAA AAAATAATAA 7965 .......... .......... .......... .......... .......... .......... 184 TTTATGTTCT AAACTACTCC TCTTTTCAAT CTCTTTATGA GTTACAAATG ACATATCTCT 7905 .......... .......... .......... .......... .......... .......... 184 TTATGAGTTA CAAATGACAT ATTTTATATT TTTAATAGTT TCTTTATTCT AGTTTTTAGT 7845 .......... .......... .......... .......... .......... .......... 184 TATAATTTTA TTTTGTATTT TATTAAGAAC TAACGTAATT TTATCCTTCT ATATTTATTT 7785 .......... .......... .......... .......... .......... .......... 184 AATATTTTTT AAAATTATAT TTATTAATAA ATAACAAAAT TCATTATTTA TTCTATTAAG 7725 .......... .......... .......... .......... .......... .......... 184 AATATAATAG GTAAAATATG ATACTTTATG CATAAAATAT ATAAAATAAT AAGTATTGCC 7665 .......... .......... .......... .......... .......... .......... 184 AAATGATTTA TAAAATGAAA TGATATATAA GATGAAATTG AAGGAGCAAT AATTTAACTT 7605 .......... .......... .......... .......... .......... .......... 184 TAGAATATTC ATTTTACTTA TAATACTCTA ATCTCTAACC ATATAAATAC CTTTGACTTA 7545 .......... .......... .......... .......... .......... .......... 184 TTTTAGATTA AATTTTTTAA AATTCTGTGT TTCTTTTTTA AATTCTATAG TAATGAATTG 7485 .......... .......... .......... .......... .......... .......... 184 AAGCTGAAAA AATATTTCTT AAGAGCAAAA AGGAATTTAA TATTAAATTA ATTTTAATTA 7425 .......... .......... .......... .......... .......... .......... 184 TAGTAAGGGA AAAAAAGGTT GACACGTAAA AGGTGAAGTA ATAAATAAAA GAATAAAATT 7365 .......... .......... .......... .......... .......... .......... 184 TTTTGTTCCT TGAATTCCTC CCTCAATTCA CGCCCAATTT TCTCTCTCTT CCCTTACCCC 7305 .......... .......... .......... .......... .......... .......... 184 AAAGGTTCCA TCTCTTTGCC CTCTTTCCAA ATCTCCCAAA TCCCCCAAAA TTCTAGGGTT 7245 .......... .......... .......... .......... .......... .......... 184 TTCATTTCCC TGTTGAAATT TGGATCGGTG CTTTGTTTTA GTTTTTGTGT AGAGGTTCCG 7185 .......... .......... .......... .......... .......... .......... 184 ATTCCTGGAT TTTGTGGGAG AATTTTGGGG GATTCCAATT GTATATTAAG AGAAAGAGAT 7125 ||| || .......... .......... .......... .......... .......... ..AAAAGTAT 192 GAGTTTTCAA GATCTTGAAG CGGGTCGACC ATTGGGGCCC CGTAGAGGGT ATATGAATGG 7065 ||||||||| ||| | ||| |||||||||| |||||| | ||| | | | ||||| GAGTTTTCAG GATTTGGAAT CGGGTCGACC TTTGGGGTCT CGTCGGTTCC AGACAAATGG 252 TAAGCAAGAC CCAACTCAAG CTGTGGCTTC TGGAATTTTC CAGATTAACA CTGCTGTGTC 7005 ||||||||| || || |||| | |||||||| |||||| ||| ||||| || | |||||||||| GAAGCAAGAC CCGACCCAAG CAGTGGCTTC TGGAATCTTC CAGATCAATA CTGCTGTGTC 312 AACGTTTCAA AGGCTGGTCA ATACACTTGG AACACCCAAA GATACACCCG AGCTGCGTGA 6945 |||||||| ||||| || | |||||||||| ||| || ||| |||||||| | |||| ||||| TACGTTTCAG AGGCTAGTGA ATACACTTGG AACCCCAAAA GATACACCTG AGCTACGTGA 372 GAAGTTGTAA GCTCTCGAAA TTTTGTACGA TAAATTGAAG TTGTAAGCTG AATTTTTGGA 6885 ||||| TAAGTT.... .......... .......... .......... .......... .......... 378 TGAAATTTTG CAATTTTTAA TACAGTTGGA ATTGCTTACA TTTCTTGTTG AATTTGATGT 6825 .......... .......... .......... .......... .......... .......... 378 TTTGTGGACT TACTGTATTG TCAATTGATG GATTAATTCT GAGACAAGTT TATAGCTTTT 6765 .......... .......... .......... .......... .......... .......... 378 GTGGTGATTG AGGTCGCACT TTAGCCATAA TCATTTATTG AGATAGACCG GGTCAACTGA 6705 .......... .......... .......... .......... .......... .......... 378 TACTGTTTTG TGGTATTTTT GTTAGGCGAT TTCATCTTTT ACTGTGAATT CGTGAATTTA 6645 .......... .......... .......... .......... .......... .......... 378 TAACTCTTCC TGTATTAGTT TTAGCGTACA CACAGAGAGT CAGAAAGAGC ATTATACTTG 6585 .......... .......... .......... .......... .......... .......... 378 AAACTGATGT TCTGCATTTG AGGAAACTCT TTAGGAAGGC CTCAGCACAT GGGCACACAC 6525 .......... .......... .......... .......... .......... .......... 378 TAAAAATGAT GTCTAAATTA TCACTGAACA TGTGCAAATG AGTTCTTCTG ATCACTTTGA 6465 .......... .......... .......... .......... .......... .......... 378 GAGTTTTGAT ATAGAGAAAA TCAGTAAGCT TGCAAAAATA TTATAAATCA AAATTTTAGA 6405 .......... .......... .......... .......... .......... .......... 378 AAACCATTTT CAGTTTTTTT GTCCACCTGT ACTTCGTGTG GATGACTGGG AAGTATATAT 6345 .......... .......... .......... .......... .......... .......... 378 TGTTCAGTAT ATGGTTCTGA TGAATGATAA AACATGTTTT TAGGCTAATT ATATACATGT 6285 .......... .......... .......... .......... .......... .......... 378 TATGGGAAAA AGTTTGAACT TTATGTTCAT GTGCCTTTTA GAGGGTTAAA TAAGGCTTTG 6225 .......... .......... .......... .......... .......... .......... 378 CTCCACTACT TACTCAAACT GGAATTGATT TACTGAAAGT ATTAACTAGT GTAACTTTTC 6165 .......... .......... .......... .......... .......... .......... 378 AAAAGAAAAG GGATTAACTA GTTGGATGTT TGCGCTTAGA CTTTTATATG AACAAATTCT 6105 .......... .......... .......... .......... .......... .......... 378 TTTAAAATGC TGCCAGCCTA CTATGTTTAA TATTGAAACT TCTCTTTGTG TGTGCATACA 6045 .......... .......... .......... .......... .......... .......... 378 GTGTTTATTG TGCAATACTC TACAAAATAG TATTCAAGTT GTTGAGTGAG TTCTTACGGG 5985 .......... .......... .......... .......... .......... .......... 378 AAGAGGTTTG CCTATCATTG CCTCTAGAAT GGGAATGTGG TTGAATGTTG CGTCGCTCAT 5925 .......... .......... .......... .......... .......... .......... 378 TCCCATGTGA TAAGTGAATG TAATATGGTT CACCGCAAAT AAGTGGGGTG AGGAGAAATG 5865 .......... .......... .......... .......... .......... .......... 378 TGATATGCGG CAATCATGAA CCGGAATCTG GCATGTTTTG ACCATCACTT GAACAGAATC 5805 .......... .......... .......... .......... .......... .......... 378 TATAGTCAAT ACTACCTTTC TGGTTCAATT TCTGTTTTCC AGCATGGTGC TGCAATAATG 5745 .......... .......... .......... .......... .......... .......... 378 AGATTCTGTT GTGTTTAGTT AGCCAATATG TGGCTTTGCT GTTATCTCGT ACTAATTAAT 5685 .......... .......... .......... .......... .......... .......... 378 ACTACCAATT AACACTGGAT TAAATATCTT AGTTTCACTC GGGGATTATG CTAACTTTCT 5625 .......... .......... .......... .......... .......... .......... 378 CAAGTACAGG CACAAGACGA GGGTACATAT TGGGCAGTTA GTGAAAGATA CATCTGCAAA 5565 | |||||||| | |||| ||||| ||| |||||| || ||||||| |||||||||| .........G CACAAGACAA GGGTTCATAT TGGCCAGTTA GTCAAAGATA CATCTGCAAA 429 ACTTAAGCAA GCCAGCGAAA CAGATCATCG TGTTGAAGTC AGTGTAAGTA TTAAGAGCTC 5505 |||||||||| || || |||| ||||||||| || ||| || ||| ACTTAAGCAA GCAAGTGAAA CAGATCATCA TGCTGATGTT AGT....... .......... 472 ACCTATTTCT TCTTCTCCTT GGTGTTTTCC TTTGTCAGTA TGGATTTCCA TGACATATAT 5445 .......... .......... .......... .......... .......... .......... 472 TTGCAGAATA CACATGCTCG TTTTCTTCTG TCCTCATTAT ATATATGAGG TTCTCATCAT 5385 .......... .......... .......... .......... .......... .......... 472 TTTTGATTGA TTAGGCTTAT GTTTACTTCT TGTTCTTCAC TCTTTAACTT TCTCTTTCAC 5325 .......... .......... .......... .......... .......... .......... 472 TATTAACTAT AAACTCCTGC TTTCTTCTAT CAGGCTAGCA AGAAAATAAC AGATGCTAAA 5265 ||||||| |||||||||| |||||||||| .......... .......... .......... ...GCTAGCA AGAAAATAAC AGATGCTAAA 499 CTTGCTAAAG ATTTTCAAGC AGTCTTGAAG GAGTTTCAAA AGGCTCAAAG GCTTGCAGCT 5205 |||||||||| |||||||||| ||||| || |||||||||| ||||||| || |||||||| CTTGCTAAAG ATTTTCAAGC TGTCTTAAAA GAGTTTCAAA AGGCTCAGAG ACTTGCAGCA 559 GAGAGAGAGA CAGCATATAC ACCTTTTGTT CC 5173 ||||| |||| |||||||||| |||||| || || GAGAGGGAGA CAGCATATAC ACCTTTCATT CC 591 hqPGS_C08HBa0217O07.1-_SGN-U315217+ (7132 6939,5615 5522,5291 5173) ******************************************************************************** EST sequence 3 +strand 1259 n (File: SGN-U318998+) 1 GGCGTCAATT TGGAAAAAGT TCACCCGTCA CCACCGTCAA CGCCGTTAAT TCTCGCCGGC 61 TCTTCATTCT CTAAACTCCG TCAACCCTCA ATTCTTCCCC ATCTCCAAAA TCCTCATAAA 121 TCCTCTCTTC TCTCTACTCC TTTGCATTTC ATTCTCTCCA ACATCTGATT TTTTCCGATC 181 TCTCATCGAA CTGCTGCGAT GTCGGTGAAA ACTGTTAAAG TTAGCAATGT CTCCTTAGGG 241 GCATCAGAGC GTGATATCAA GGAATTCTTT TCTTTCTCTG GTGATATTGA ATATGTTGAG 301 ATGCAAAGTG ATACTGAACG AAGTCAAATA GCATATGTTA CGTTCAATGA TTCTCAGGGA 361 GCTGACACTG CAGTTCTTCT TTCGGGTGCA ACAATAGTTG ATATGTCTGT TACGGTGACT 421 TTGGATCCAG TTTACCAGCT TCCTCCTACT GCTTTTACAG CATCAGTGCC AACTGGAAAG 481 AAAAGTGCTG GTAATTCAGA ATCCGCTTTT CAGAAAGCAG AAGACGTTGT TAGCAGCATG 541 CTCGCAAAAG GTTATATCTT AGGTAAAGAT GCTGTAGGGA AAGCAAAGTC TTTTGATGAG 601 AAACACCAGT TGACCTCAAC TGCAACTGCT AAAGTGGCCT CTCTCGACAA AAAAATTGGT 661 CTCACTGAAA AGATAAGCAT TGGTACTTCC ATTGTTAATG ACAAAGTTCG AGAGGTGGAT 721 CAGAAACTCC AAGTTTCTGA TAAAGCAAAA TCAGCACTTT CTGCAGCTGA GCAAACAGTT 781 AGTAGCGCTG GATCAGCCAT TATGAAAAAT CGGTATGTTC TGACTGGGTC CACTTGGGTA 841 GCCGGCGCTT TTAGTAAGGT TGCTAAGGCA GCAGGGGAAG TAGGCCAGAT GACAAAAGAG 901 AAAGTGGGAA TGACAGAGGA TGAGCAAAGG CAGAAAATGG TTACTGATTT CGCACAGGTT 961 CATTTATCTG AGTCTCCGAA AGCTTCCGAG TTCACAGAAC ATCAGCCAGC CAAGCCTGCA 1021 CCAGTACAAG GTTTAGTCCT TTGAGTTGAA GGCCCTTGTA AGAACTATCT TTGAAGACTT 1081 CCACCTCGTA CTACATTTCA TTTTGCAATC CACTGTTTTA TAAAGAAATG GCAAATGTAA 1141 AACAGTATGT CCAATACAAA TATTTAGATG ATTTATTTTG TATTTGTGCT TTTTACTGCT 1201 ACAGANATTT GATTGAATTG AACATCAANT GGTATTTCCA AGTAGATAAA AAAAAGAAA Predicted gene structure (within gDNA segment 12277 to 18993): Exon 1 12877 13080 ( 204 n); cDNA 1 204 ( 204 n); score: 1.000 Intron 1 13081 14983 (1903 n); Pd: 0.941 (s: 1.00), Pa: 0.760 (s: 1.00) Exon 2 14984 15087 ( 104 n); cDNA 205 308 ( 104 n); score: 1.000 Intron 2 15088 15188 ( 101 n); Pd: 0.859 (s: 1.00), Pa: 0.971 (s: 1.00) Exon 3 15189 15264 ( 76 n); cDNA 309 384 ( 76 n); score: 1.000 Intron 3 15265 17352 (2088 n); Pd: 0.856 (s: 1.00), Pa: 0.965 (s: 1.00) Exon 4 17353 17436 ( 84 n); cDNA 385 468 ( 84 n); score: 1.000 Intron 4 17437 17513 ( 77 n); Pd: 0.934 (s: 1.00), Pa: 0.988 (s: 1.00) Exon 5 17514 18293 ( 780 n); cDNA 469 1250 ( 782 n); score: 0.994 MATCH C08HBa0217O07.1+ SGN-U318998+ 0.996 1248 0.991 C PGS_C08HBa0217O07.1+_SGN-U318998+ (12877 13080,14984 15087,15189 15264,17353 17436,17514 18293) Alignment (genomic DNA sequence = upper lines): GGCGTCAATT TGGAAAAAGT TCACCCGTCA CCACCGTCAA CGCCGTTAAT TCTCGCCGGC 12936 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCGTCAATT TGGAAAAAGT TCACCCGTCA CCACCGTCAA CGCCGTTAAT TCTCGCCGGC 60 TCTTCATTCT CTAAACTCCG TCAACCCTCA ATTCTTCCCC ATCTCCAAAA TCCTCATAAA 12996 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTCATTCT CTAAACTCCG TCAACCCTCA ATTCTTCCCC ATCTCCAAAA TCCTCATAAA 120 TCCTCTCTTC TCTCTACTCC TTTGCATTTC ATTCTCTCCA ACATCTGATT TTTTCCGATC 13056 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCTCTCTTC TCTCTACTCC TTTGCATTTC ATTCTCTCCA ACATCTGATT TTTTCCGATC 180 TCTCATCGAA CTGCTGCGAT GTCGGTGAGT AGAAGCTACA TTTTGAGTAT TCCACTTGCG 13116 |||||||||| |||||||||| |||| TCTCATCGAA CTGCTGCGAT GTCG...... .......... .......... .......... 204 ATTTTGTTTT TCAAGTTTTG AACGGATTTT TCTGCTGGAT ATTCATCTGA TTTGATAGCT 13176 .......... .......... .......... .......... .......... .......... 204 TTGAAGGTTG CTTCTATTGA TTGCTTTTGT TGAAGTTCAG CTACGATTTT TTCTTGTCTT 13236 .......... .......... .......... .......... .......... .......... 204 CTTAAATCTT CGAGCTCTGA GATTGAGATG TTTTGAATTA TCTGATTACT TCAAATATAA 13296 .......... .......... .......... .......... .......... .......... 204 GCTACATTTT GAGCATTCAT GTTGCGATTC TGTTTTATAA GTTTTTAACG GAATTTTCTG 13356 .......... .......... .......... .......... .......... .......... 204 CTGTATATTC GTCTGATTTG ATAGTTTTGG ATGTTGCTTC TGTTGATTGC CTTTGTTGAA 13416 .......... .......... .......... .......... .......... .......... 204 GTTCAGCTAC GATTATCTTT GAGCTCTGAG ATTGAGATGA TTTGAAGTGT CTAATTACTT 13476 .......... .......... .......... .......... .......... .......... 204 CAATTATAAG CTAATATAGC TTTTTAAGTA TATTTGAAAT ATTTATGTTA CTCGTATATT 13536 .......... .......... .......... .......... .......... .......... 204 GTTATTGATT TGCTTGAAAT CGTCAAAGTT GAAGCCATCA AAAGGAGTAT AAGCGACATT 13596 .......... .......... .......... .......... .......... .......... 204 GTTGGAAAAT TTGCTGTAAT AGTATAAAAG TATAAGATTT GCGTTTATTT AAAAAGTTGA 13656 .......... .......... .......... .......... .......... .......... 204 CTAGTTAAAA AAAAATCCTG GAAAATGTTA GTTTTATGCC TTTTTCATCT GAACAAATAC 13716 .......... .......... .......... .......... .......... .......... 204 TGTGTATTAT CGGAGTTCTG GCTTATATGC GACATATTCT GCATTTGATT AATATAGTAA 13776 .......... .......... .......... .......... .......... .......... 204 TCATATCATA AGTTGACCAA GTATGGATCT CTAGCTAGTT GGAGTCGACT AGTCGACTAG 13836 .......... .......... .......... .......... .......... .......... 204 ATCCCCTCTA CATTCAGTCA ACTCTATTTG AGGCCGTATC ATTCATGTAC TTGTATATAA 13896 .......... .......... .......... .......... .......... .......... 204 AAAAATTCTG ATGTCTAGCC AGCTTAAAGA TAATTGTAAT TCGATGAATC CTCTATTCTG 13956 .......... .......... .......... .......... .......... .......... 204 CGGATCATGC AAATAAACAA TTCCTGCACA AACAATTTGA ACTTTTACAA AAACATGTTT 14016 .......... .......... .......... .......... .......... .......... 204 GGATTATGAG TATACTGACA TGCATTTTCT GGCTCAAACT GTAATTATTT GACTACTATT 14076 .......... .......... .......... .......... .......... .......... 204 TCTAGGCCAG ATCACTTTTT TATTTTTATT AGAGGAATAT TATATTCCTA TATACATGTG 14136 .......... .......... .......... .......... .......... .......... 204 TGTTAGAAAG GAGAGGCTGG AGGAGATAAG ATCGGTCATC CTCAGATTTG AGGTGGCGTA 14196 .......... .......... .......... .......... .......... .......... 204 GTATAATACT AGCTTTGCTA CTTCCCATGC TGGTTTCCTA AATTGTGCGT CAATTATGTG 14256 .......... .......... .......... .......... .......... .......... 204 AAATTTTGTG CCGTCAATTA TGTGAAATTT TGTGCTTAAG GAGACCAAAG AAGTTAGACC 14316 .......... .......... .......... .......... .......... .......... 204 AACATCTTCA TATGCAATGT TAAGTCACAG GTGGATGTAC AACGTCTTTA AGGCCAAGGG 14376 .......... .......... .......... .......... .......... .......... 204 CATTTTCATA CATGAGATAT CAAATAAAAG TTGATTTAAT ATTCATAATT GCAATTTCAT 14436 .......... .......... .......... .......... .......... .......... 204 GACGTTCTTC TAAAATGACT AGTTCAAAAA ACATTACTTT GCTCTTTGTG TGAAAGAGTA 14496 .......... .......... .......... .......... .......... .......... 204 TTACAATAAC TACTAGGCTT TGGTCCCAAA TAAGTTGGGG TCATCTAATT CAAAACGAAG 14556 .......... .......... .......... .......... .......... .......... 204 ATATGAAAGA GTATAAATTA TCACTAAATT ACATTTCCAA CAATGTCAAG CAGCAAAAAT 14616 .......... .......... .......... .......... .......... .......... 204 GGAAAAATGC ATGTTGAATA GGAATAGTGG AGTTGGGGAG TATGTTAATT AGTTTGCGGA 14676 .......... .......... .......... .......... .......... .......... 204 AAGGGATCAA AATAAACGGG TGTAACTTCT CGTTTAATCA GAATCAATCA ACCTAATGCT 14736 .......... .......... .......... .......... .......... .......... 204 CAAACATTTG CTGATAAGTT TCAATTCAAG ACCTCTCTCA TCATATCTTA CTCCTAATAG 14796 .......... .......... .......... .......... .......... .......... 204 TCCCAAATCT TCATTTTAAA TTACTATTAC ACATCGTCGC ATGATCGTTT TGCTTTGCAT 14856 .......... .......... .......... .......... .......... .......... 204 TTTCTATTTT GTTTTAAAGG ATGATAAATG TTGTTCGAAT ATGTCTTATT GTGGATTACA 14916 .......... .......... .......... .......... .......... .......... 204 TATATAACTG AAATGTTGAA AATGAGACTG TCTTCCCAAG CTCTTGACAG TGTGCTTTCA 14976 .......... .......... .......... .......... .......... .......... 204 TGAGCAGGTG AAAACTGTTA AAGTTAGCAA TGTCTCCTTA GGGGCATCAG AGCGTGATAT 15036 ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......GTG AAAACTGTTA AAGTTAGCAA TGTCTCCTTA GGGGCATCAG AGCGTGATAT 257 CAAGGAATTC TTTTCTTTCT CTGGTGATAT TGAATATGTT GAGATGCAAA GGTTAGCGCT 15096 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CAAGGAATTC TTTTCTTTCT CTGGTGATAT TGAATATGTT GAGATGCAAA G......... 308 TGAGGTTGTT TACCATTTTC TTTGAGTGAA TTCGTTCGTT AGTTTGTCAT TAACACCACT 15156 .......... .......... .......... .......... .......... .......... 308 TTTTTTTTTT TATCTTTTTT CTTACTTTGT AGTGATACTG AACGAAGTCA AATAGCATAT 15216 |||||||| |||||||||| |||||||||| .......... .......... .......... ..TGATACTG AACGAAGTCA AATAGCATAT 336 GTTACGTTCA ATGATTCTCA GGGAGCTGAC ACTGCAGTTC TTCTTTCGGT TATCATCGTT 15276 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| GTTACGTTCA ATGATTCTCA GGGAGCTGAC ACTGCAGTTC TTCTTTCG.. .......... 384 CTCCCTATCA GTCAAATTTT ACAATTTGTT TCCCTTACTC TTTGCATTAT GCTTATTTGT 15336 .......... .......... .......... .......... .......... .......... 384 GGCTGGGACA TCAAGCTGAT AATTTGAAAT CTGAAGATTG ACCATTGCAA CAATGTTTCC 15396 .......... .......... .......... .......... .......... .......... 384 TCCTATGCGC ATAAGTGCAT TTATATCAAG TAGTATTCAT GATTATTTTC TTCATTAATG 15456 .......... .......... .......... .......... .......... .......... 384 TTCTGGCGAA TTACTATTTG TCATTTTAGC ATGAGACTGA GATGGTAGTT TTCACTGCTA 15516 .......... .......... .......... .......... .......... .......... 384 GAACAGTTAT TTTCTAGTAA CGAACCTCCA TCATGGTCTC GTGCCTTCTT TATCTCGTCA 15576 .......... .......... .......... .......... .......... .......... 384 ATATGCTGTT TGCCGGCAGA TTTCTTTGCC ATGTTTGACT CTCTCTGTGT GTGTGTATAT 15636 .......... .......... .......... .......... .......... .......... 384 ATATATATAT GTATGTATGG ATATAATTTT TATTTTATGT TTGACTGTAT AAAGATATGA 15696 .......... .......... .......... .......... .......... .......... 384 AGTAATGACT TAAGCTAATT GACGATGCAA TTGCCAGACA AAAAACTTTT TATTTGAGGC 15756 .......... .......... .......... .......... .......... .......... 384 CATTGTATGT ATTCATTTAG ATAGTTCTTT GTTTTAGTCA TTTAAATTTT TAAAGCTAAT 15816 .......... .......... .......... .......... .......... .......... 384 TTAAATTCTT GAAATTAGTG CTACTACATA GCCTGGCATT GGGGATGTTG CTTTCGGTTT 15876 .......... .......... .......... .......... .......... .......... 384 TCATCTGCAG AAGTCCAGGT TCTCACTTAA ATGCTTATCT AAGCATGAGA GTTTGATTGT 15936 .......... .......... .......... .......... .......... .......... 384 AGGGCAGACG ATTTTACTGC AGGGTTCCCT TGAAAATACT AGGAGCGAGT TTGGCTAACT 15996 .......... .......... .......... .......... .......... .......... 384 CACCATATTT GTGTTCCCAA GATAAGTTAT CATATAAATA TTATTGGAGA AAAGGGAAAA 16056 .......... .......... .......... .......... .......... .......... 384 TAAACTTTCT TAGGGCTGTT GGAATTGTGT AAAATTCTGC TTAAATAGGT TATAAGGAGG 16116 .......... .......... .......... .......... .......... .......... 384 GAGGTGCAAA GCTGAAAGTC TGAAGTTTTT ATAAGGAAGA TCAAAGATGG TGTTCAGTTG 16176 .......... .......... .......... .......... .......... .......... 384 TAGTAATTCT TGATTTATGT TGATTACGGA AGAATCTTAT TTTAGGTTCT ATACACAAAC 16236 .......... .......... .......... .......... .......... .......... 384 TTAACTCTTC TCCTAATGTC AATTGCTCAT ATGTTTATTA GTTTGATGTT ATGCTATTTC 16296 .......... .......... .......... .......... .......... .......... 384 TCCATCAAAG ACCGTTAAGT CTGGTGATCT GCACAAAATT GTCAGATACT TCCAAAACTT 16356 .......... .......... .......... .......... .......... .......... 384 TGTTAGATAC TTGTAAAAGT TTCTCTATCC TTTTTCGTTC TAGGGAAGAT GTTAGTGTTC 16416 .......... .......... .......... .......... .......... .......... 384 AATGGAATGT TAACATGAGC TAGCACTTGG TAAATAGACT GAACACTGAA CTGGAGGTTT 16476 .......... .......... .......... .......... .......... .......... 384 AACATCAGGT CAGCATTGAG TCCATACTGT GTGAAGGCTT AAATGGAAGG CCATTCATGT 16536 .......... .......... .......... .......... .......... .......... 384 TGGTCTCCTT CTCTTCCTAG GTATTGTTGG GCTTTCTAGG ATCAGAGGGT ATTCAATGAA 16596 .......... .......... .......... .......... .......... .......... 384 TGCAACCTTT GGCTTATGTC TTTTTTAAAA AAAATTATAA AACTGGTTTC TGGGTCATCT 16656 .......... .......... .......... .......... .......... .......... 384 GGGGTAAACT AAACTATTCA GCTAGATACC TGCATCCTCC TGCCATGGCA GGTTCAAGGT 16716 .......... .......... .......... .......... .......... .......... 384 ATCTTTGCCT ATCACGCCTT AGGCAGATTG AAAGAAATCA GTTGACTTGT TTGCCCCTTG 16776 .......... .......... .......... .......... .......... .......... 384 CTGTGAATCG AACACTGTCT GTTGCAATTT CTTTTCCAGC TTCCTTGACC ATTAGGTCAG 16836 .......... .......... .......... .......... .......... .......... 384 AGATTTGGGT GTGAGCTTTA GCAGATCCCT AAAATGATAA TGTAAACTCT TTCAAGTTAT 16896 .......... .......... .......... .......... .......... .......... 384 TATTTCATAT TATTATGATG CAAGTGAGGT ATTAATTATG CCAGAAGCAC CATACTGCAT 16956 .......... .......... .......... .......... .......... .......... 384 TTCAGAACAG TGTCATGTAT ATCCTCAATG CTTACCTCTA CCCTGTAACA TTTGAAGTTT 17016 .......... .......... .......... .......... .......... .......... 384 ATATGTTGAC AATTCATCTT AAAAAGGGTA TTAACAGTTA TGATGTAATT CTTATGCAAA 17076 .......... .......... .......... .......... .......... .......... 384 CAAGTGTATT CTTCCAAGTG GTTGATTTCA TTTTCTTTCG AGCCTTTTCC TGCAAATTCT 17136 .......... .......... .......... .......... .......... .......... 384 TTTGTAAGGG CTCAAAAATT CTGATACATC GTGAACTTTT CTGGAAGCAA GAAAGTGTCG 17196 .......... .......... .......... .......... .......... .......... 384 GTGGCAATAC TTGTGAAATA AATTTGTAGT TTTTCTCACT TTATTCCTAA TGGCTATCAC 17256 .......... .......... .......... .......... .......... .......... 384 TTCTCCCTTT CAATGTGGAG ATTGTGGTAG GCCTTGAGTG TTTACAAACA TAGAAACAAG 17316 .......... .......... .......... .......... .......... .......... 384 AAAGAGTTCT CAATAGTGTG ATTGTATTTA TTTCAGGGTG CAACAATAGT TGATATGTCT 17376 |||| |||||||||| |||||||||| .......... .......... .......... ......GGTG CAACAATAGT TGATATGTCT 408 GTTACGGTGA CTTTGGATCC AGTTTACCAG CTTCCTCCTA CTGCTTTTAC AGCATCAGTG 17436 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTACGGTGA CTTTGGATCC AGTTTACCAG CTTCCTCCTA CTGCTTTTAC AGCATCAGTG 468 GTAAAAAGAA ATAATATACA TACATGTCCT TTGAACATGA AAGAACACTT TTCTTATATG 17496 .......... .......... .......... .......... .......... .......... 468 ATATTTGTGA TCTGCAGCCA ACTGGAAAGA AAAGTGCTGG TAATTCAGAA TCCGCTTTTC 17556 ||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .......CCA ACTGGAAAGA AAAGTGCTGG TAATTCAGAA TCCGCTTTTC 511 AGAAAGCAGA AGACGTTGTT AGCAGCATGC TCGCAAAAGG TTATATCTTA GGTAAAGATG 17616 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAAGCAGA AGACGTTGTT AGCAGCATGC TCGCAAAAGG TTATATCTTA GGTAAAGATG 571 CTGTAGGGAA AGCAAAGTCT TTTGATGAGA AACACCAGTT GACCTCAACT GCAACTGCTA 17676 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTAGGGAA AGCAAAGTCT TTTGATGAGA AACACCAGTT GACCTCAACT GCAACTGCTA 631 AAGTGGCCTC TCTCGACAAA AAAATTGGTC TCACTGAAAA GATAAGCATT GGTACTTCCA 17736 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGGCCTC TCTCGACAAA AAAATTGGTC TCACTGAAAA GATAAGCATT GGTACTTCCA 691 TTGTTAATGA CAAAGTTCGA GAGGTGGATC AGAAACTCCA AGTTTCTGAT AAAGCAAAAT 17796 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTTAATGA CAAAGTTCGA GAGGTGGATC AGAAACTCCA AGTTTCTGAT AAAGCAAAAT 751 CAGCACTTTC TGCAGCTGAG CAAACAGTTA GTAGCGCTGG ATCAGCCATT ATGAAAAATC 17856 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGCACTTTC TGCAGCTGAG CAAACAGTTA GTAGCGCTGG ATCAGCCATT ATGAAAAATC 811 GGTATGTTCT GACTGGGTCC ACTTGGGTAG CCGGCGCTTT TAGTAAGGTT GCTAAGGCAG 17916 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTATGTTCT GACTGGGTCC ACTTGGGTAG CCGGCGCTTT TAGTAAGGTT GCTAAGGCAG 871 CAGGGGAAGT AGGCCAGATG ACAAAAGAGA AAGTGGGAAT GACAGAGGAT GAGCAAAGGC 17976 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGGGGAAGT AGGCCAGATG ACAAAAGAGA AAGTGGGAAT GACAGAGGAT GAGCAAAGGC 931 AGAAAATGGT TACTGATTTC GCACAGGTTC ATTTATCTGA GTCTCCGAAA GCTTCCGAGT 18036 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAAATGGT TACTGATTTC GCACAGGTTC ATTTATCTGA GTCTCCGAAA GCTTCCGAGT 991 TCACAGAACA TCAGCCAGCC AAGCCTGCAC CAGTACAAGG TTTAGTCCTT TGAGTTGAAG 18096 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCACAGAACA TCAGCCAGCC AAGCCTGCAC CAGTACAAGG TTTAGTCCTT TGAGTTGAAG 1051 GCCCTTGTAA GAACTATCTT TGAAGACTTC CACCTCGTAC TACATTTCAT TTTGCAATCC 18156 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCCCTTGTAA GAACTATCTT TGAAGACTTC CACCTCGTAC TACATTTCAT TTTGCAATCC 1111 ACTGTTTTAT AAAGAAATGG CAAATGTAAA ACAGTATGTC CAATACAAAT ATTTAGATGA 18216 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTGTTTTAT AAAGAAATGG CAAATGTAAA ACAGTATGTC CAATACAAAT ATTTAGATGA 1171 TTTATTTTGT ATTTGTGCTT TTTACTGCTA CAGA-ATTTG ATTGAATTGA ACATCAA-TG 18274 |||||||||| |||||||||| |||||||||| |||| ||||| |||||||||| ||||||| || TTTATTTTGT ATTTGTGCTT TTTACTGCTA CAGANATTTG ATTGAATTGA ACATCAANTG 1231 GTATTTCCAA GTAGATAAA 18293 |||||||||| ||||||||| GTATTTCCAA GTAGATAAA 1250 hqPGS_C08HBa0217O07.1+_SGN-U318998+ (12877 13080,14984 15087,15189 15264,17353 17436,17514 18293) ******************************************************************************** EST sequence 9 +strand 966 n (File: SGN-U329136+) 1 AGGATTTGCG AGTAAATCCC CAGACTACTA AATAACCTCA ACAGCTACCA AACACTCGCT 61 TCGCGCACTC TTTCTCAATC GCCGTTTCTC TCTCATTCTC GAATCTCCAG AATCCGGCCA 121 ACATGCATAG ACAATCACTC GGCTCATCGG GATCGAAGTT ACACCTTGCA CATGGAGTCG 181 TCCTCGTCGG CGGCAGCAGG GACGAATCCG CCGTCGTGAC GGCGGCAGAG TCTCAGAAGA 241 TAATGGCGAA GGACCAAGCC TCTCCGTCGT CACTCTCTAA CAACTACGAT GAAGGAGAAG 301 AACAAGTACG CAAGTCAATC AAGGCTCTTA ACAAGTCATT GTCTAGAGCT GAGAAGTACA 361 TTCACCTCAT TCCTGTTCTC ACATTTCTCT GCTTCTTCAT CCTCTATCTA TTCTCTCACA 421 GTCCGTCTGA TAAAGATTTA GCTCAATTTC AAGGATTTGA AGGCTTCGCT AAGCGTATAG 481 AATCAGCAAA TATTGACGAC GAGTTGCAGA GAGTATTAGA AACTAAAAAA CCGGAAGTTT 541 TAGCGATCCG AAGCGTAAGG AACTTGCAAG AGATTGATAG ACAGGATTCA AATCATCGGC 601 GCCACCGAAA ACTCGCCGAT TTTTAAGCCG CTGTTTGTGC TTCTTACTCT TCTTTATTCT 661 CCGTATATTA CTCAACCACC TATTCATGGC GAATTCACCT TCGGCGGAGC TTCTCTCACG 721 TGCGTAACTC GTTTCCTTGC CAATTTTACC GTTTCATTAT TACCACGCCT GTGTGCATGT 781 GAATTTCTTA TACTAGTAAT TTTGGCTGTT TTGAAACAAA TCAAATTTAC CGGTGAAGTA 841 CTCTGTAAAT ATTTTACTTT ACTATATATG ATGTACCAAA AAAGCAAAAT ATTCACTTCC 901 AAGTTTTGGA TACGTTAAAT GGAAATTTCT GAGCTGGAGC TGGAAACTAG AAAGGGAAAA 961 AAAAAA Predicted gene structure (within gDNA segment 26446 to 23862): Exon 1 25816 25385 ( 432 n); cDNA 4 435 ( 432 n); score: 1.000 Intron 1 25384 25311 ( 74 n); Pd: 0.886 (s: 1.00), Pa: 0.976 (s: 1.00) Exon 2 25310 25266 ( 45 n); cDNA 436 480 ( 45 n); score: 1.000 Intron 2 25265 25057 ( 209 n); Pd: 0.787 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 3 25056 24571 ( 486 n); cDNA 481 966 ( 486 n); score: 0.988 MATCH C08HBa0217O07.1- SGN-U329136+ 0.993 963 0.997 C PGS_C08HBa0217O07.1-_SGN-U329136+ (25816 25385,25310 25266,25056 24571) Alignment (genomic DNA sequence = upper lines): ATTTGCGAGT AAATCCCCAG ACTACTAAAT AACCTCAACA GCTACCAAAC ACTCGCTTCG 25757 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTGCGAGT AAATCCCCAG ACTACTAAAT AACCTCAACA GCTACCAAAC ACTCGCTTCG 63 CGCACTCTTT CTCAATCGCC GTTTCTCTCT CATTCTCGAA TCTCCAGAAT CCGGCCAACA 25697 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGCACTCTTT CTCAATCGCC GTTTCTCTCT CATTCTCGAA TCTCCAGAAT CCGGCCAACA 123 TGCATAGACA ATCACTCGGC TCATCGGGAT CGAAGTTACA CCTTGCACAT GGAGTCGTCC 25637 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCATAGACA ATCACTCGGC TCATCGGGAT CGAAGTTACA CCTTGCACAT GGAGTCGTCC 183 TCGTCGGCGG CAGCAGGGAC GAATCCGCCG TCGTGACGGC GGCAGAGTCT CAGAAGATAA 25577 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGTCGGCGG CAGCAGGGAC GAATCCGCCG TCGTGACGGC GGCAGAGTCT CAGAAGATAA 243 TGGCGAAGGA CCAAGCCTCT CCGTCGTCAC TCTCTAACAA CTACGATGAA GGAGAAGAAC 25517 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGCGAAGGA CCAAGCCTCT CCGTCGTCAC TCTCTAACAA CTACGATGAA GGAGAAGAAC 303 AAGTACGCAA GTCAATCAAG GCTCTTAACA AGTCATTGTC TAGAGCTGAG AAGTACATTC 25457 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTACGCAA GTCAATCAAG GCTCTTAACA AGTCATTGTC TAGAGCTGAG AAGTACATTC 363 ACCTCATTCC TGTTCTCACA TTTCTCTGCT TCTTCATCCT CTATCTATTC TCTCACAGTC 25397 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCTCATTCC TGTTCTCACA TTTCTCTGCT TCTTCATCCT CTATCTATTC TCTCACAGTC 423 CGTCTGATAA AGGTACACTG CATCTTAGTT TCGTGATTCA GAGAAAAAAC GAACTCGTTT 25337 |||||||||| || CGTCTGATAA AG........ .......... .......... .......... .......... 435 ACTGATTTAC ATGTTTTTTG TTTCAGATTT AGCTCAATTT CAAGGATTTG AAGGCTTCGC 25277 |||| |||||||||| |||||||||| |||||||||| .......... .......... ......ATTT AGCTCAATTT CAAGGATTTG AAGGCTTCGC 469 TAAGCGTATA GGTATGCATA TTGCGCATTT TGTTTCGCCG TTCCGCAATT GTTTCAATTT 25217 |||||||||| | TAAGCGTATA G......... .......... .......... .......... .......... 480 AGATTTCAGA TTTAGTTCCA TACTTCCATT ATACTCAAGT ATTCATCAAT TTCAATTCAA 25157 .......... .......... .......... .......... .......... .......... 480 AAAAATCGGC TCTCAATGTG TTTTACATAC TTCTCAGATG CCTCGTGATC CGTATTTTGC 25097 .......... .......... .......... .......... .......... .......... 480 TTGCTCTAAT GAGTTCTCTC TTTTTGCTCT GTATATACAG AATCAGCAAA TATTGACGAC 25037 |||||||||| |||||||||| .......... .......... .......... .......... AATCAGCAAA TATTGACGAC 500 GAGTTGCAGA GAGTATTAGA AACTAAAAAA CCGGAAGTTT TAGCGATCCG AAGCGTAAGG 24977 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGTTGCAGA GAGTATTAGA AACTAAAAAA CCGGAAGTTT TAGCGATCCG AAGCGTAAGG 560 AACTTGCAAG AGATTGATAG ACAGGATTCA AATCATCGGC GCCACCGAAA ACTCGCCGAT 24917 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACTTGCAAG AGATTGATAG ACAGGATTCA AATCATCGGC GCCACCGAAA ACTCGCCGAT 620 TTTTAAGCCG CTGTTTGTGC TTCTTACTCT TCTTTATTCT CCGTATATTA CTCAACCACC 24857 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTAAGCCG CTGTTTGTGC TTCTTACTCT TCTTTATTCT CCGTATATTA CTCAACCACC 680 TATTCATGGC GAATTCACCT TCGGCGGAGC TTCTCTCACG TGCGTAACTC GTTTCCTTGC 24797 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATTCATGGC GAATTCACCT TCGGCGGAGC TTCTCTCACG TGCGTAACTC GTTTCCTTGC 740 CAATTTTACC GTTTCATTAT TACCACGCCT GTGTGCATGT GAATTTCTTA TACTAGTAAT 24737 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAATTTTACC GTTTCATTAT TACCACGCCT GTGTGCATGT GAATTTCTTA TACTAGTAAT 800 TTTGGCTGTT TTGAACAAAA TCAAATTTAC CGGTGAAGTA CTCTGTAAAT ATTTTACTTT 24677 |||||||||| ||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGGCTGTT TTGAAACAAA TCAAATTTAC CGGTGAAGTA CTCTGTAAAT ATTTTACTTT 860 ACTATATATG ATGTACCAAA AAAGCAAAAT ATTCACTTCC AAGTTTTGGA TACGTTAAAT 24617 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTATATATG ATGTACCAAA AAAGCAAAAT ATTCACTTCC AAGTTTTGGA TACGTTAAAT 920 GGAAATTTCT GAGCTGGAGC TGGAAACTAG AAAGGAAAAT ATCAAA 24571 |||||||||| |||||||||| |||||||||| ||||| ||| | ||| GGAAATTTCT GAGCTGGAGC TGGAAACTAG AAAGGGAAAA AAAAAA 966 hqPGS_C08HBa0217O07.1-_SGN-U329136+ (25816 25385,25310 25266,25056 24571) ******************************************************************************** EST sequence 4 +strand 1033 n (File: SGN-U321737+) 1 AGGGACTACT TTAACTTGCC TTGTTGAAAA CGTAAACGGA AACGCCATAT TTTCTCATAA 61 AGCTTGAAGA AGAAAAACAG ATTTTTTTTT TTTGGCCTGA AAAAGAGACT GATAGAGGAA 121 GAAGAAAGTT CTAGAAAGAA AATGGTTCTA TGGGAGATCA CATTGGGAAC GGCGTATTTT 181 TTGGGTCTGA AGCGAACTTA CAGGCTGTTC TTGAGGATTC AGCGTAAGCT CATTAGTCCA 241 AAGTATCCTA GGCTTCGTGA TTTCGCTCAA AGGCGAACAC GAGCTGCATT TGATATGGCA 301 CTGATCGTTC ATCGCAAGGT ACAGGAAAGA GATATTGAAG CTGGTCGGAA CCTTGGTAAT 361 TGGATCCTTC GATGGCTTGA CAAAATGAAG CCATCTGCTA ATATTCGAGG CTCAGATAAC 421 ATTGCAATTA CCAGTGCAAC TAAGCAGCTT AGGAACTCAT CTCACTCCCC CAAGCCTGAA 481 GCTTTCCAGA AATACGGTGC TGGCAAAGAC AAGGAATCCA GCAGCAGGCA TCTGTTTACC 541 TCAGCTAGTA ACACATGGCA GAAAGCATAC CCGACCATTT CCATGATGAT GAAACCAAGA 601 AGCCCAGCTG GAACAAATAT CCAATACAGG CAGTTAAACA CTGTTTCGCC TACTAGTTTC 661 AAACCCATGA AACATGGTTT TGAGGGAGTG ATCCGACCGG ACATATTGCA GTGGATTCAA 721 CGAAGCTAAT CCATTAACTA CTCTGTTGCT GCCTTTTGGC TTTTACACTA GTGGACTTGA 781 ACGTCTGCAG AAGGTGCATA TACGGCACAA ATAAAAGCTC ACTAGATCAG TGCCGTCATG 841 AAAAAATTTT AGTCTCTGTT TTCTTTTGGC TGAACAGTCA ATATTGTACA TTCGTTTCAG 901 ATTCTGACCT GTGTGTACCT CAACCAACCT TGATTCTTCT GTTGTTTCTA CTCTGTTGTA 961 GCCTCTAATG CAATAACAAA TAGTATTGAA GTCAATTTTT TTTGTTAAAA AAAAAAAAAA 1021 AAAAAAAAAA AAA Predicted gene structure (within gDNA segment 27971 to 33081): Exon 1 28571 28841 ( 271 n); cDNA 1 272 ( 272 n); score: 0.991 Intron 1 28842 31476 (2635 n); Pd: 0.994 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 2 31477 32211 ( 735 n); cDNA 273 1007 ( 735 n); score: 1.000 PPA cDNA 1008 1033 MATCH C08HBa0217O07.1+ SGN-U321737+ 0.998 1006 0.974 C PGS_C08HBa0217O07.1+_SGN-U321737+ (28571 28841,31477 32211) Alignment (genomic DNA sequence = upper lines): AGGGACTACT TTAACTTGCC TTGTTGAAAA CGTAAACGGA AACGCCATAT TTTCTCATAA 28630 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGGACTACT TTAACTTGCC TTGTTGAAAA CGTAAACGGA AACGCCATAT TTTCTCATAA 60 AGCTTGAAGA AGAAAAACAG A-TTTTTTTT TTTGGCCTGA AAAAGAGACT GATAGAGGAA 28689 |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| AGCTTGAAGA AGAAAAACAG ATTTTTTTTT TTTGGCCTGA AAAAGAGACT GATAGAGGAA 120 GAAGAAAGTT CTAGAAAGAA AATGGTTCTA TGGGAGATCA CATTGGGAAC GGCGTATTTT 28749 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGAAAGTT CTAGAAAGAA AATGGTTCTA TGGGAGATCA CATTGGGAAC GGCGTATTTT 180 TTGGGTCTGA AGCGAACTTA CAGGCTGTTC TTGAGGATTC AGCGTAAGCT CATTAGTCCA 28809 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGGGTCTGA AGCGAACTTA CAGGCTGTTC TTGAGGATTC AGCGTAAGCT CATTAGTCCA 240 AAGTATCCTA GGCTTCGTGA TTTCGCTCAA AGGTATTTTG TTATTTGAAT TGGATCTGAT 28869 |||||||||| |||||||||| |||||||||| || AAGTATCCTA GGCTTCGTGA TTTCGCTCAA AG........ .......... .......... 272 TTTGATCTCT AATGATATTT TTTTTTTGTG ATTATTCGGT TTAGTTTTTC TTAGCTTATA 28929 .......... .......... .......... .......... .......... .......... 272 TAATTTATCA ATCCGTTGAC TGGAAATAGA GACAAGCAAT TTGATTCAAT GTGATTGATT 28989 .......... .......... .......... .......... .......... .......... 272 CTACAGTGTA TACACTCAAT TTCAGGGCAA GTCTCAGAGA GGAAAGTGAA AAATTAGTAT 29049 .......... .......... .......... .......... .......... .......... 272 CTGGAGAAAC AGCTTCATAA TTAATCATAC AATAAGCAAT AGCATATGAG TGTTTGCAGC 29109 .......... .......... .......... .......... .......... .......... 272 GGTTGCCATT GTCATTGTCC TCGTGCTTTT AGGTAAATGA GGGGAATGGA GAGCAAGAGG 29169 .......... .......... .......... .......... .......... .......... 272 CTAAAGATAG GTTACTGTGG GATATTAATG TACATGGTCT CTGCATTTTA TCTAATGATT 29229 .......... .......... .......... .......... .......... .......... 272 GAAAAACTAG GGTAATAGGA ATGAGAGAGG AGAGATAGGA GGAGGGGAGA AAGACGAGTG 29289 .......... .......... .......... .......... .......... .......... 272 AGAGAGAGGA TAAGAATGAG AAAAGAGGCA AGGATAGAAG ACAAAGTTGA ATTTGAGGCA 29349 .......... .......... .......... .......... .......... .......... 272 AACTTTTTTT GCTATCAGGA CTGGGAGAAC CATGAGAGAA AGAGAGTGTG AGAGACATTG 29409 .......... .......... .......... .......... .......... .......... 272 GAAGAGTAAT AGTAGGGTAA AACTGATTGG GAGTTATTAT TACGATAAAA CTGATTGAGA 29469 .......... .......... .......... .......... .......... .......... 272 GACTAATGTA TCGACAAAAG CGATCATCTA TTGTTACTAC TCTCGGCTTT CTATGGAGCA 29529 .......... .......... .......... .......... .......... .......... 272 ACACTACTAA TAGAAAGGAA GGTGAAAAGA AGTTTAGGAC AATGTTGGAA AAAGAGAGAA 29589 .......... .......... .......... .......... .......... .......... 272 GCTTAGGATG ACTCAGCGTC CTTGGCGGAC ATTTCCAATT TCAGGGCCCA GTTTGAATGC 29649 .......... .......... .......... .......... .......... .......... 272 ATATGCAGTT TCATGTTCTA GTATTTGACA TAGTTTCTAT AGGACTCGGT GACAAACTTG 29709 .......... .......... .......... .......... .......... .......... 272 TCACTTTTCC CCAATGCAAA TGGAGAAGTT GTCTTGGAGG AGTCCGTCAA GAGGAGAACT 29769 .......... .......... .......... .......... .......... .......... 272 TCACATAGGT GTCTAAGCTC GAAATGTTAT CTTGGTCATT TTCTTATTGT TATATCTTCA 29829 .......... .......... .......... .......... .......... .......... 272 TCAACTTTTA TTGTTATTTT AGCTCAGCGT AATGGTTCTT GATTGTAGTA CTATACAGTT 29889 .......... .......... .......... .......... .......... .......... 272 CTTTCTCCTA AATGACCTTT TTTGTCATGA TACCCATCCC ATCATCCCAT GTATCTTTCT 29949 .......... .......... .......... .......... .......... .......... 272 GCTTTTAGTC ATTCTCAGTG ACAATGGTTG CTGTTCTTTC AAATAAAACG GCAAAAGTTT 30009 .......... .......... .......... .......... .......... .......... 272 GCTATTCCAG CTTCAACCAC TTGATTTTGT TCTTATTTGT TTGAGTATTG TTGATATGGA 30069 .......... .......... .......... .......... .......... .......... 272 TTTTCAGATC CATTGTTTTG TTCCCAACTA TTGAATAAAG GTCTCTTAGC TTGGAAATTA 30129 .......... .......... .......... .......... .......... .......... 272 CTTATTTTTG TGTTTAAAAT GAACTATCTT CTAGTAAGTG GTAATGGTGG ATTCAATGTC 30189 .......... .......... .......... .......... .......... .......... 272 CCATTTCTCA GTTTTGTCTC TCGGACTCCC CCGACATTTG ACAATCTTGT TGGAATAAGA 30249 .......... .......... .......... .......... .......... .......... 272 TTGTTTCAGG TACATCTCTT TCTTTTGTTC CTGGAATTTC GTGAAAGTTA AATTTTCTAT 30309 .......... .......... .......... .......... .......... .......... 272 AAGCAATGGG ACTGGAATAT ATGCTTGTCT TATATCTCAA GAACAGTTGC TTGGTAAATA 30369 .......... .......... .......... .......... .......... .......... 272 CTTTTAGAGC TGATGATGCA TGTAGGTGGT GAGGTGAGCA CTTTTCAGTT TAACATGCAA 30429 .......... .......... .......... .......... .......... .......... 272 CACTGGCCTA TATTTCCTGG ATTTGTGTAG GCACGTTAGG TCATCCATTA GATCATTTAT 30489 .......... .......... .......... .......... .......... .......... 272 GTGTTTTAAG GTGTTAGATT TGCCAAATCC TTTATGTTCA AGATGGAAGG ATATGGATAA 30549 .......... .......... .......... .......... .......... .......... 272 AGATGCTGCA TGCATGTCTG AGAAAATTTA AAGTAAATAT ATAAATGATA AAGAGTGGAT 30609 .......... .......... .......... .......... .......... .......... 272 TCTTTTACTG TTTAAGGAAA ATGAGGATAA TAAAATTAGA AGAGAACTTT TGATATTTCT 30669 .......... .......... .......... .......... .......... .......... 272 CTTCATCAAA ATCTATGAGT TAGTTTACTG TCTGAGTTGG TAGTAGATGT CAAAATATCT 30729 .......... .......... .......... .......... .......... .......... 272 CAATTAATAT GACCAAACTA ACCACATCTT CACCACATTG TCCATGTTAT GCCATGTTGA 30789 .......... .......... .......... .......... .......... .......... 272 AATAGTATAC TGTTCAATCA ATGATTCTGA GTGATATAAA AGCATAATTT CTCTTGTTCA 30849 .......... .......... .......... .......... .......... .......... 272 CGCCTTGTAT GAACAACTGT TGATATGAGG AGGAAGAAGA GGCTAGATAT AGCTTTTAAG 30909 .......... .......... .......... .......... .......... .......... 272 TCAGCAGGAT GAGCTGAGAG TGAATATTCT TCATGACAAC AATCAGTAAT GTAGGGCCCG 30969 .......... .......... .......... .......... .......... .......... 272 GAAGAAAATG TTGTGTGTGT GTTGATACAA GGTAGTATAT CCATGACATG AACTGTGGCA 31029 .......... .......... .......... .......... .......... .......... 272 AATTTAATTA GGTATTTGTT GTCAAGCGTG TACACATCCA AGTTTATCAT GAATATGAAT 31089 .......... .......... .......... .......... .......... .......... 272 CTGTGTTGAG CTTTATATGG TATATTTAGT ATGTAATTCC TCAATTTTCT TGTTACGGTA 31149 .......... .......... .......... .......... .......... .......... 272 GTGAGATCAA ATTCTTCACC TTGTGGAAGT GTTAATGTCT AATGTTTACT GGTCCTGTTT 31209 .......... .......... .......... .......... .......... .......... 272 CAACACACAA TATTTTCACA TTGTCTCTAC CTTTTTTATT CTTCATATTT GAGATGTACC 31269 .......... .......... .......... .......... .......... .......... 272 CTGTCACCTG TTGATTGGAT AATATTCAAG GTTGTTAATA TTGCCAGATT AGCCTAAGGA 31329 .......... .......... .......... .......... .......... .......... 272 TGGTTTTTCC CTGTTGTTCG ATGCTTGTTT ATGATCTTTT CTGTCCAAAA TTGATTATGC 31389 .......... .......... .......... .......... .......... .......... 272 TTGTTTAGGA TCTTTTCTTT CTACTATTTA TTGCTTTGTT CCTTCATCCT TGTATTATTG 31449 .......... .......... .......... .......... .......... .......... 272 TTTTTCAAAT ATTTCTTTCT TTTCCAGGCG AACACGAGCT GCATTTGATA TGGCACTGAT 31509 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......GCG AACACGAGCT GCATTTGATA TGGCACTGAT 305 CGTTCATCGC AAGGTACAGG AAAGAGATAT TGAAGCTGGT CGGAACCTTG GTAATTGGAT 31569 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGTTCATCGC AAGGTACAGG AAAGAGATAT TGAAGCTGGT CGGAACCTTG GTAATTGGAT 365 CCTTCGATGG CTTGACAAAA TGAAGCCATC TGCTAATATT CGAGGCTCAG ATAACATTGC 31629 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCTTCGATGG CTTGACAAAA TGAAGCCATC TGCTAATATT CGAGGCTCAG ATAACATTGC 425 AATTACCAGT GCAACTAAGC AGCTTAGGAA CTCATCTCAC TCCCCCAAGC CTGAAGCTTT 31689 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATTACCAGT GCAACTAAGC AGCTTAGGAA CTCATCTCAC TCCCCCAAGC CTGAAGCTTT 485 CCAGAAATAC GGTGCTGGCA AAGACAAGGA ATCCAGCAGC AGGCATCTGT TTACCTCAGC 31749 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCAGAAATAC GGTGCTGGCA AAGACAAGGA ATCCAGCAGC AGGCATCTGT TTACCTCAGC 545 TAGTAACACA TGGCAGAAAG CATACCCGAC CATTTCCATG ATGATGAAAC CAAGAAGCCC 31809 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGTAACACA TGGCAGAAAG CATACCCGAC CATTTCCATG ATGATGAAAC CAAGAAGCCC 605 AGCTGGAACA AATATCCAAT ACAGGCAGTT AAACACTGTT TCGCCTACTA GTTTCAAACC 31869 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTGGAACA AATATCCAAT ACAGGCAGTT AAACACTGTT TCGCCTACTA GTTTCAAACC 665 CATGAAACAT GGTTTTGAGG GAGTGATCCG ACCGGACATA TTGCAGTGGA TTCAACGAAG 31929 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATGAAACAT GGTTTTGAGG GAGTGATCCG ACCGGACATA TTGCAGTGGA TTCAACGAAG 725 CTAATCCATT AACTACTCTG TTGCTGCCTT TTGGCTTTTA CACTAGTGGA CTTGAACGTC 31989 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTAATCCATT AACTACTCTG TTGCTGCCTT TTGGCTTTTA CACTAGTGGA CTTGAACGTC 785 TGCAGAAGGT GCATATACGG CACAAATAAA AGCTCACTAG ATCAGTGCCG TCATGAAAAA 32049 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCAGAAGGT GCATATACGG CACAAATAAA AGCTCACTAG ATCAGTGCCG TCATGAAAAA 845 ATTTTAGTCT CTGTTTTCTT TTGGCTGAAC AGTCAATATT GTACATTCGT TTCAGATTCT 32109 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTTAGTCT CTGTTTTCTT TTGGCTGAAC AGTCAATATT GTACATTCGT TTCAGATTCT 905 GACCTGTGTG TACCTCAACC AACCTTGATT CTTCTGTTGT TTCTACTCTG TTGTAGCCTC 32169 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACCTGTGTG TACCTCAACC AACCTTGATT CTTCTGTTGT TTCTACTCTG TTGTAGCCTC 965 TAATGCAATA ACAAATAGTA TTGAAGTCAA TTTTTTTTGT TA 32211 |||||||||| |||||||||| |||||||||| |||||||||| || TAATGCAATA ACAAATAGTA TTGAAGTCAA TTTTTTTTGT TA 1007 hqPGS_C08HBa0217O07.1+_SGN-U321737+ (28571 28841,31477 32211) ******************************************************************************** EST sequence 2 +strand 883 n (File: SGN-U337363+) 1 GAGGAAGATC CTATAGGGCG ATTGGGTACC GGGCCCCCCC TCGAGTTTTT TTTTTTTTTT 61 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT AAAAAAAAGT AACTACCCTT TTATTATTTA 121 TCACAATCAT TCAATAACCA AAAAAAAAAA AAAAATTAGG GAGAAAAAAG GGAAAATATT 181 ATTATGGATC AGGGGAAAAA GCTTTACGTT CTTGGCGCTG GCTCTTACGG CAAAGTTTAT 241 TATGCAGGGA AAAGGGATCC GGTTTCGTTA TGGGTTTCTG TTGCGGCTGT TAAATGGGCT 301 GATATGAACC GTTCTGTTTC ACTCCAACGA AAAGCAAAAA TCTTGACAAC CCTCAAAAAT 361 TCCCCTTACG GGGTTCAGTT CATTGGATCA AACGTTAGCA TCGATAACGG TAATGTCCCA 421 ACTTACAATC TGTTTCTTGA GTATGCATAT GGGGGATCCC TACCCAATTT GATCATTAAT 481 TCAAAAATGA AAAGGATACG GATGTCTGAA GTGGAAGGAG GGGGCGATGC ATATCAACTC 541 TTAAAGGGTA TTCCACCCGT TCATGAAAAA GGGTGGGTTC ATTGGGAAAT TAAACCTGCC 601 AATGTTTTGA TTTTCCACAA CCCTGAACGG GGGGGGGAAG CCCAATTTAA AGTTAGCTGA 661 CTCTGGATTG TCCTTGAAAA TTCCGAGAAG GGGGGGAATA AATACCGGGG GCTGCACAGA 721 ACAAAAAAGT GACGCCAGCC ATCCCCCCCC CCAAACATGT GCACTCCTGT GTCTCCGTTG 781 CGGCGGTTTG TAGCTCACAG ATCTCGTGCT AACACGGTGA GAGGGACGCT AACATGTCTC 841 GCGAAATGAC ACACAGTCTA GTGACGACGA TGAGTCTATA TCG Predicted gene structure (within gDNA segment 33721 to 36199): Exon 1 35261 35934 ( 674 n); cDNA 77 753 ( 677 n); score: 0.878 MATCH C08HBa0217O07.1+ SGN-U337363+ 0.878 674 0.763 C PGS_C08HBa0217O07.1+_SGN-U337363+ (35261 35934) Alignment (genomic DNA sequence = upper lines): TTTTTTTTCT CTATAAAAAG AAGTAACTAC CCTTTTATTA TTTATCACAA TCATTCAATA 35320 |||||||| | | |||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTTTTTT TTTTAAAAAA AAGTAACTAC CCTTTTATTA TTTATCACAA TCATTCAATA 136 ACCAAAAAAA AAAAAAAAAT TAGTGAGAAG AAAGTGAAAA TATTATTATG GATCAGTGGA 35380 |||||||||| |||||||||| ||| ||||| |||| ||||| |||||||||| |||||| ||| ACCAAAAAAA AAAAAAAAAT TAGGGAGAAA AAAGGGAAAA TATTATTATG GATCAGGGGA 196 AGAAGCTTTA CGTTCTTGGC GCTGGCTCTT ACGGCAAAGT TTATTATGCA GTGAAGATGG 35440 | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| | ||| | || AAAAGCTTTA CGTTCTTGGC GCTGGCTCTT ACGGCAAAGT TTATTATGCA GGGAAAAGGG 256 ATCCTGTTTC GTTATGTGTT TCTGTTGCTG CTGTTAAATG TGCTGATATG AACCGTTCTG 35500 |||| ||||| |||||| ||| |||||||| | |||||||||| ||||||||| |||||||||| ATCCGGTTTC GTTATGGGTT TCTGTTGCGG CTGTTAAATG GGCTGATATG AACCGTTCTG 316 TTTCACTCCA ACGAGAAGCA GAAATCTTGA CAACCCTCAA AGATTCACCT TACGTGGTTC 35560 |||||||||| |||| ||||| ||||||||| |||||||||| | |||| ||| |||| ||||| TTTCACTCCA ACGAAAAGCA AAAATCTTGA CAACCCTCAA AAATTCCCCT TACGGGGTTC 376 AGTTCATTGG ATCAGACGTT AGCATCGATA ACGGTAATGT CTCAACTTAC AATCTGTTTC 35620 |||||||||| |||| ||||| |||||||||| |||||||||| | |||||||| |||||||||| AGTTCATTGG ATCAAACGTT AGCATCGATA ACGGTAATGT CCCAACTTAC AATCTGTTTC 436 TTGAGTATGC ATATGGTGGA TCGCTACACG ATTTGATCAT TAATTCGAAG ATGAGAAGGA 35680 |||||||||| |||||| ||| || |||| | |||||||||| |||||| || |||| ||||| TTGAGTATGC ATATGGGGGA TCCCTACCCA ATTTGATCAT TAATTCAAAA ATGAAAAGGA 496 TACGGATGTC TGAAGTGGAA GTAGGTTTCT ATGCATATCA ACTCTTAAAG GGTATTCAAC 35740 |||||||||| |||||||||| | ||| | |||||||||| |||||||||| ||||||| || TACGGATGTC TGAAGTGGAA GGAGGGGGCG ATGCATATCA ACTCTTAAAG GGTATTCCAC 556 ACGTTCATGA GAAAGGGTGG GTTCATTGTG ATATTAAACC TGCTAATGTT TTGATTTTCA 35800 ||||||||| ||||||||| |||||||| | | |||||||| ||| |||||| ||||||||| CCGTTCATGA AAAAGGGTGG GTTCATTGGG AAATTAAACC TGCCAATGTT TTGATTTTCC 616 ACAACGCTGA AC-GTGGTGG GATGCACAAG TTAAAGTTGG CTGACTTTGG ATTGTCGTTG 35859 ||||| |||| || | || || || || ||| |||||||| | |||||| ||| |||||| ||| ACAACCCTGA ACGGGGGGGG GAAGCCCAAT TTAAAGTTAG CTGACTCTGG ATTGTCCTTG 676 AGAGTTCC-C GAAGGTGTGG CATATATGAC TGGTGCTGCG ATGAGCAACA GAGGGACTCT 35918 | | |||| ||||| | || ||| || | || ||||| || ||| | || ||| | AAAATTCCGA GAAGGGGGGG AATAAATACC GGGGGCTGCA CAGAACAAAA AAGTGACGCC 736 A-CCTTACGC GCCCCCA 35934 | || | | | |||||| AGCCATCCCC CCCCCCA 753 hqPGS_C08HBa0217O07.1+_SGN-U337363+ (35261 35934) ******************************************************************************** EST sequence 10 -strand 660 n (File: SGN-U321387-) 1 GTTAAATGTG CTGATATGAA CCGTTCTGTT TCACTCCAAC GAGAAGCAGA AATCTTGACA 61 ACCCTCAAAG ATTCACCTTA CGTGGTTCAG TTCATTGGAT CAGACGTTAG CATCGATAAC 121 GGTAATGTCT CAACTTACAA TCTGTTTCTT GAGTATGCAT ATGGTGGATC GCTACACGAT 181 TTGATCATTA ATTCGAAGAT GAGAAGGATA CGGATGTCTG AAGTGGAAGT AGGTTTCTAT 241 GCATATCAAC TCTTAAAGGG TATTCAACAC GTTCATGAGA AAGGGTGGGT TCATTGTGAT 301 ATTAAACCTG CTAATGTTTT GATTTTCAAC AACGCTGAAC GTGGTGGGAT GCACAAGTTA 361 AAGTTGGCTG ACTTTGGATT GTCGTTGAGA GTTCCCGAAG GTGTGGCATA TATGACTGGT 421 GCTGCGATGA GCAACAGAGG GACTCTACCT TACGCGCCCC CAGAATCTTT GATCAGTGGT 481 TTTCATGGCA GGAGTTATGA TATATGGTCG TTAGGGTGTA CTGTGGCAGA GATGATGACT 541 GGGTGTCGCG TTTGGATTTA TCGCGACACT AAGGATTTGC AATGGAAGAT TATGAATGAA 601 GAACCAATGG TTCCTAGTGA TGTTTCTGAG ATTGCCAGAG ATTTTTTGTA CAAGTGTTTG Predicted gene structure (within gDNA segment 34863 to 36199): Exon 1 35473 36132 ( 660 n); cDNA 1 660 ( 660 n); score: 1.000 MATCH C08HBa0217O07.1+ SGN-U321387- 1.000 660 1.000 C PGS_C08HBa0217O07.1+_SGN-U321387- (35473 36132) Alignment (genomic DNA sequence = upper lines): GTTAAATGTG CTGATATGAA CCGTTCTGTT TCACTCCAAC GAGAAGCAGA AATCTTGACA 35532 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTAAATGTG CTGATATGAA CCGTTCTGTT TCACTCCAAC GAGAAGCAGA AATCTTGACA 60 ACCCTCAAAG ATTCACCTTA CGTGGTTCAG TTCATTGGAT CAGACGTTAG CATCGATAAC 35592 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCCTCAAAG ATTCACCTTA CGTGGTTCAG TTCATTGGAT CAGACGTTAG CATCGATAAC 120 GGTAATGTCT CAACTTACAA TCTGTTTCTT GAGTATGCAT ATGGTGGATC GCTACACGAT 35652 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTAATGTCT CAACTTACAA TCTGTTTCTT GAGTATGCAT ATGGTGGATC GCTACACGAT 180 TTGATCATTA ATTCGAAGAT GAGAAGGATA CGGATGTCTG AAGTGGAAGT AGGTTTCTAT 35712 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGATCATTA ATTCGAAGAT GAGAAGGATA CGGATGTCTG AAGTGGAAGT AGGTTTCTAT 240 GCATATCAAC TCTTAAAGGG TATTCAACAC GTTCATGAGA AAGGGTGGGT TCATTGTGAT 35772 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATATCAAC TCTTAAAGGG TATTCAACAC GTTCATGAGA AAGGGTGGGT TCATTGTGAT 300 ATTAAACCTG CTAATGTTTT GATTTTCAAC AACGCTGAAC GTGGTGGGAT GCACAAGTTA 35832 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTAAACCTG CTAATGTTTT GATTTTCAAC AACGCTGAAC GTGGTGGGAT GCACAAGTTA 360 AAGTTGGCTG ACTTTGGATT GTCGTTGAGA GTTCCCGAAG GTGTGGCATA TATGACTGGT 35892 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTTGGCTG ACTTTGGATT GTCGTTGAGA GTTCCCGAAG GTGTGGCATA TATGACTGGT 420 GCTGCGATGA GCAACAGAGG GACTCTACCT TACGCGCCCC CAGAATCTTT GATCAGTGGT 35952 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTGCGATGA GCAACAGAGG GACTCTACCT TACGCGCCCC CAGAATCTTT GATCAGTGGT 480 TTTCATGGCA GGAGTTATGA TATATGGTCG TTAGGGTGTA CTGTGGCAGA GATGATGACT 36012 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCATGGCA GGAGTTATGA TATATGGTCG TTAGGGTGTA CTGTGGCAGA GATGATGACT 540 GGGTGTCGCG TTTGGATTTA TCGCGACACT AAGGATTTGC AATGGAAGAT TATGAATGAA 36072 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGGTGTCGCG TTTGGATTTA TCGCGACACT AAGGATTTGC AATGGAAGAT TATGAATGAA 600 GAACCAATGG TTCCTAGTGA TGTTTCTGAG ATTGCCAGAG ATTTTTTGTA CAAGTGTTTG 36132 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAACCAATGG TTCCTAGTGA TGTTTCTGAG ATTGCCAGAG ATTTTTTGTA CAAGTGTTTG 660 hqPGS_C08HBa0217O07.1+_SGN-U321387- (35473 36132) Total number of EST alignments reported: 11 ________________________________________________________________________________ Predicted gene locations (7) in segment 1 to 36199: PGL 1 (+ strand): 131 817 AGS-1 (131 369,570 817) SCR (e 0.992 d 0.999 a 0.814,e 0.964) Exon 1 131 369 ( 239 n); score: 0.992 Intron 1 370 569 ( 200 n); Pd: 0.999 Pa: 0.814 Exon 2 570 817 ( 248 n); score: 0.964 PGS (131 369,570 817) SGN-U327248+ 3-phase translation of AGS-1 (+strand): . . . . . . 131 ATTAGATTTTATGGGTTTCTAGCACTGCTACCCGTCATTTTGAGGCTTGTTGTTGTCCAC I R F Y G F L A L L P V I L R L V V V H L D F M G F - H C Y P S F - G L L L S T - I L W V S S T A T R H F E A C C C P . . . . . . 191 GTTTTGTTGTAGTTTTCAGGGATGCTGCCGTATTTGGAGATACAAATTTAGAGTTGCCTT V L L - F S G M L P Y L E I Q I - S C L F C C S F Q G C C R I W R Y K F R V A F R F V V V F R D A A V F G D T N L E L P . . . . . . 251 CTTTTCTGTCAAACCGCTCGCTTGTTGAGGTTCATCTGGAAGTGGGTACCAATTTAACAT L F C Q T A R L L R F I W K W V P I - H F S V K P L A C - G S S G S G Y Q F N I S F L S N R S L V E V H L E V G T N L T . . . . . . : 311 CACAACAGAAGGATGAACTGGAAATACACATGGAACTTCCTCTACATGCACGCTATCAG : C H N R R M N W K Y T W N F L Y M H A I S : T T E G - T G N T H G T S S T C T L S : A S Q Q K D E L E I H M E L P L H A R Y Q : . . . . . . 571 CACTAGGACATGGATTCTCAAGAGTTGAATTTGCTTCACCTGACTTATTTCTGCGCTGCA H - D M D S Q E L N L L H L T Y F C A A T R T W I L K S - I C F T - L I S A L Q P L G H G F S R V E F A S P D L F L R C . . . . . . 631 ACCTTGAAAGAAAAGAATATGACACGGGCTGTTTGTTTCTGCTAGACAAACAGAATGCTG T L K E K N M T R A V C F C - T N R M L P - K K R I - H G L F V S A R Q T E C - N L E R K E Y D T G C L F L L D K Q N A . . . . . . 691 AATCAATTGATACTCACCCTGTGTGGGAAGTACCATGTGGAAATCGAGAACACACTGAAG N Q L I L T L C G K Y H V E I E N T L K I N - Y S P C V G S T M W K S R T H - S E S I D T H P V W E V P C G N R E H T E . . . . . . 751 TTGTATCTGCTTTTACATTCATCTCAGCTCTTGTATCAGCTCTTCTCATCATTGTTGCCT L Y L L L H S S Q L L Y Q L F S S L L P C I C F Y I H L S S C I S S S H H C C L V V S A F T F I S A L V S A L L I I V A . 811 CAATTAA Q L N - S I Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1+_PGL-1_AGS-1_PPS_1 (136 369,570 815) (frame '0'; 480 bp, 160 residues) 1 ILWVSSTATR HFEACCCPRF VVVFRDAAVF GDTNLELPSF LSNRSLVEVH LEVGTNLTSQ 61 QKDELEIHME LPLHARYQPL GHGFSRVEFA SPDLFLRCNL ERKEYDTGCL FLLDKQNAES 121 IDTHPVWEVP CGNREHTEVV SAFTFISALV SALLIIVASI PGL 2 (- strand): 2140 1959 AGS-1 (2140 1959) SCR (e 0.912) Exon 1 2140 1959 ( 182 n); score: 0.912 PGS (2140 1959) SGN-U331953+ 3-phase translation of AGS-1 (-strand): . . . . . . 2140 TTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATATGTGGACCAT L A K I K C S I C S Y Q F N I - Y V D H W L R S S V V S V L I S L I S D M W T I G - D Q V - Y L F L S V - Y L I C G P . . . . . . 2080 TGGTTCACATGATATTAACTCAATTTTTTAAGGGGAAGAGTCCATCAAGGTAGCTTGCTG W F T - Y - L N F L R G R V H Q G S L L G S H D I N S I F - G E E S I K V A C C L V H M I L T Q F F K G K S P S R - L A . . . . . . 2020 CCTGGGCTCTTGCGCGTCGCCCATGCGTTGCACTACTGCACGGGCCTGGCGCACCCCACC P G L L R V A H A L H Y C T G L A H P T L G S C A S P M R C T T A R A W R T P P A W A L A R R P C V A L L H G P G A P H . 1960 AA Q Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (+strand): . . . . . . 1959 TTGGTGGGGTGCGCCAGGCCCGTGCAGTAGTGCAACGCATGGGCGACGCGCAAGAGCCCA L V G C A R P V Q - C N A W A T R K S P W W G A P G P C S S A T H G R R A R A Q G G V R Q A R A V V Q R M G D A Q E P . . . . . . 2019 GGCAGCAAGCTACCTTGATGGACTCTTCCCCTTAAAAAATTGAGTTAATATCATGTGAAC G S K L P - W T L P L K K L S - Y H V N A A S Y L D G L F P L K N - V N I M - T R Q Q A T L M D S S P - K I E L I S C E . . . . . . 2079 CAATGGTCCACATATCAGATATTAAACTGATAAGAACAGATACTACACTTGATCTTAGCC Q W S T Y Q I L N - - E Q I L H L I L A N G P H I R Y - T D K N R Y Y T - S - P P M V H I S D I K L I R T D T T L D L S . 2139 AA Q Maximal non-overlapping open reading frames (>= 64 codons): none PGL 3 (- strand): 7343 3092 AGS-1 (7343 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4145,3591 3092) SCR (e 1.000 d 0.983 a 0.923,e 1.000 d 0.996 a 0.999,e 1.000 d 1.000 a 0.990,e 1.000 d 0.999 a 0.998,e 1.000 d 0.996 a 0.998,e 1.000 d 0.974 a 0.917,e 0.980) Exon 1 7343 6939 ( 405 n); score: 1.000 Intron 1 6938 5616 (1323 n); Pd: 0.983 Pa: 0.923 Exon 2 5615 5522 ( 94 n); score: 1.000 Intron 2 5521 5292 ( 230 n); Pd: 0.996 Pa: 0.999 Exon 3 5291 5152 ( 140 n); score: 1.000 Intron 3 5151 4759 ( 393 n); Pd: 1.000 Pa: 0.990 Exon 4 4758 4684 ( 75 n); score: 1.000 Intron 4 4683 4484 ( 200 n); Pd: 0.999 Pa: 0.998 Exon 5 4483 4326 ( 158 n); score: 1.000 Intron 5 4325 4246 ( 80 n); Pd: 0.996 Pa: 0.998 Exon 6 4245 4145 ( 101 n); score: 1.000 Intron 6 4144 3592 ( 553 n); Pd: 0.974 Pa: 0.917 Exon 7 3591 3092 ( 500 n); score: 0.980 PGS (7343 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4145,3591 3092) SGN-U315215+ PGS (7192 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4151) SGN-U315218+ PGS (7126 6939,5615 5522,5291 5152,4758 4702) SGN-U315216+ PGS (7132 6939,5615 5522,5291 5173) SGN-U315217+ 3-phase translation of AGS-1 (-strand): . . . . . . 7343 CTCAATTCACGCCCAATTTTCTCTCTCTTCCCTTACCCCAAAGGTTCCATCTCTTTGCCC L N S R P I F S L F P Y P K G S I S L P S I H A Q F S L S S L T P K V P S L C P Q F T P N F L S L P L P Q R F H L F A . . . . . . 7283 TCTTTCCAAATCTCCCAAATCCCCCAAAATTCTAGGGTTTTCATTTCCCTGTTGAAATTT S F Q I S Q I P Q N S R V F I S L L K F L S K S P K S P K I L G F S F P C - N L L F P N L P N P P K F - G F H F P V E I . . . . . . 7223 GGATCGGTGCTTTGTTTTAGTTTTTGTGTAGAGGTTCCGATTCCTGGATTTTGTGGGAGA G S V L C F S F C V E V P I P G F C G R D R C F V L V F V - R F R F L D F V G E W I G A L F - F L C R G S D S W I L W E . . . . . . 7163 ATTTTGGGGGATTCCAATTGTATATTAAGAGAAAGAGATGAGTTTTCAAGATCTTGAAGC I L G D S N C I L R E R D E F S R S - S F W G I P I V Y - E K E M S F Q D L E A N F G G F Q L Y I K R K R - V F K I L K . . . . . . 7103 GGGTCGACCATTGGGGCCCCGTAGAGGGTATATGAATGGTAAGCAAGACCCAACTCAAGC G S T I G A P - R V Y E W - A R P N S S G R P L G P R R G Y M N G K Q D P T Q A R V D H W G P V E G I - M V S K T Q L K . . . . . . 7043 TGTGGCTTCTGGAATTTTCCAGATTAACACTGCTGTGTCAACGTTTCAAAGGCTGGTCAA C G F W N F P D - H C C V N V S K A G Q V A S G I F Q I N T A V S T F Q R L V N L W L L E F S R L T L L C Q R F K G W S . . . . . : . 6983 TACACTTGGAACACCCAAAGATACACCCGAGCTGCGTGAGAAGTT : GCACAAGACGAGGGT Y T W N T Q R Y T R A A - E V : A Q D E G T L G T P K D T P E L R E K L : H K T R V I H L E H P K I H P S C V R S : C T R R G . . . . . . 5600 ACATATTGGGCAGTTAGTGAAAGATACATCTGCAAAACTTAAGCAAGCCAGCGAAACAGA T Y W A V S E R Y I C K T - A S Q R N R H I G Q L V K D T S A K L K Q A S E T D Y I L G S - - K I H L Q N L S K P A K Q . . : . . . . 5540 TCATCGTGTTGAAGTCAGT : GCTAGCAAGAAAATAACAGATGCTAAACTTGCTAAAGATTT S S C - S Q : C - Q E N N R C - T C - R F H R V E V S : A S K K I T D A K L A K D F I I V L K S V : L A R K - Q M L N L L K I . . . . . . 5250 TCAAGCAGTCTTGAAGGAGTTTCAAAAGGCTCAAAGGCTTGCAGCTGAGAGAGAGACAGC S S S L E G V S K G S K A C S - E R D S Q A V L K E F Q K A Q R L A A E R E T A F K Q S - R S F K R L K G L Q L R E R Q . . . . : . . 5190 ATATACACCTTTTGTTCCCCAAGCAGTTCTTCCTTCAAG : TTATACAGCCAGTGAGATAGA I Y T F C S P S S S S F K : L Y S Q - D R Y T P F V P Q A V L P S S : Y T A S E I D H I H L L F P K Q F F L Q : V I Q P V R - . . . . . . : 4737 TGTTTCTTCGGGAAAGAGTCCAGAACAGCGAGCTCTCCTTGTGGAATCAAGAAG : ACAAGA C F F G K E S R T A S S P C G I K K : T R V S S G K S P E Q R A L L V E S R R : Q E M F L R E R V Q N S E L S L W N Q E : D K . . . . . . 4477 GGTTTTACTTTTGGACAACGAGATAGCCTTTAATGAAGCAATCATCGAAGAAAGAGAGCA G F T F G Q R D S L - - S N H R R K R A V L L L D N E I A F N E A I I E E R E Q R F Y F W T T R - P L M K Q S S K K E S . . . . . . 4417 GGGAATACAAGAAATACAACAACAAATTGGTGAAGTGAATGAAATTTTCAAGGACCTTGC G N T R N T T T N W - S E - N F Q G P C G I Q E I Q Q Q I G E V N E I F K D L A R E Y K K Y N N K L V K - M K F S R T L . . . . : . . 4357 TGTGCTTGTTCATGAGCAAGGAGCTATGATTG : ATGATATTGGTTCTAACGTTGAGAATGC C A C S - A R S Y D - : - Y W F - R - E C V L V H E Q G A M I : D D I G S N V E N A L C L F M S K E L - L : M I L V L T L R M . . . . . . 4217 TCATGCTGCGACTGCACAGGGGAGATCGCAACTTGCTAAAGCTGCAAAGACCCAAAGATC S C C D C T G E I A T C - S C K D P K I H A A T A Q G R S Q L A K A A K T Q R S L M L R L H R G D R N L L K L Q R P K D . . : . . . . 4157 AAATTCATCTCTG : ACTTGCTTACTCCTGGTGATTTTCGGAATCGTGCTCCTCATTGTGAT K F I S : D L L T P G D F R N R A P H C D N S S L : T C L L L V I F G I V L L I V I Q I H L - : L A Y S W - F S E S C S S L - . . . . . . 3544 CGTAGTACTTGCAGCCTGAGCTGAAGATTACTGGGATATAGAGAAGGGTCCCGAACTCTG R S T C S L S - R L L G Y R E G S R T L V V L A A - A E D Y W D I E K G P E L C S - Y L Q P E L K I T G I - R R V P N S . . . . . . 3484 TTCAGACACAGAAGTGAGTGCTTTGATTCGAAAAAAAGTGACTGTTATGATTCGAGTCAT F R H R S E C F D S K K S D C Y D S S H S D T E V S A L I R K K V T V M I R V I V Q T Q K - V L - F E K K - L L - F E S . . . . . . 3424 TTCAATAGGCATCCTGGAGTGTGGCTCGGTTGTCCCAACCCTTGTTCTTCTTGTCATGGT F N R H P G V W L G C P N P C S S C H G S I G I L E C G S V V P T L V L L V M V F Q - A S W S V A R L S Q P L F F L S W . . . . . . 3364 TGATAAGTCGTGTAGTTAGGGAGGTGGGATAAGAAAGGTTCCTGTTCAACTTGTAAACCT - - V V - L G R W D K K G S C S T C K P D K S C S - G G G I R K V P V Q L V N L L I S R V V R E V G - E R F L F N L - T . . . . . . 3304 GGTGCTGTGTTTGCAAGATGTATTGCTGACATGTATTGGTGGGTCTGTCAATAACAGTTT G A V F A R C I A D M Y W W V C Q - Q F V L C L Q D V L L T C I G G S V N N S L W C C V C K M Y C - H V L V G L S I T V . . . . . . 3244 ATTTTTGTTTGAGGAGCAATCTCTTATATTTGCCCTTTGGATACTCTTGAACCAGATGAC I F V - G A I S Y I C P L D T L E P D D F L F E E Q S L I F A L W I L L N Q M T Y F C L R S N L L Y L P F G Y S - T R - . . . . . . 3184 ATTCAATTCTTTATTGATGCTTGTGTGTGTGTGAAGTTATCACTAATGGGATATGTTGAT I Q F F I D A C V C V K L S L M G Y V D F N S L L M L V C V - S Y H - W D M L I H S I L Y - C L C V C E V I T N G I C - . . . . 3124 TTGTTTATCTTGTCTGGTGGATTATCTTGCATG L F I L S G G L S C M C L S C L V D Y L A F V Y L V W W I I L H Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1-_PGL-3_AGS-1_PPS_1 (7135 6939,5615 5522,5291 5152,4758 4684,4483 4326,4245 4145,3591 3526) (frame '2'; 828 bp, 276 residues) 1 EKEMSFQDLE AGRPLGPRRG YMNGKQDPTQ AVASGIFQIN TAVSTFQRLV NTLGTPKDTP 61 ELREKLHKTR VHIGQLVKDT SAKLKQASET DHRVEVSASK KITDAKLAKD FQAVLKEFQK 121 AQRLAAERET AYTPFVPQAV LPSSYTASEI DVSSGKSPEQ RALLVESRRQ EVLLLDNEIA 181 FNEAIIEERE QGIQEIQQQI GEVNEIFKDL AVLVHEQGAM IDDIGSNVEN AHAATAQGRS 241 QLAKAAKTQR SNSSLTCLLL VIFGIVLLIV IVVLAA- >C08HBa0217O07.1-_PGL-3_AGS-1_PPS_2 (7343 7107) (frame '1'; 234 bp, 78 residues) 1 LNSRPIFSLF PYPKGSISLP SFQISQIPQN SRVFISLLKF GSVLCFSFCV EVPIPGFCGR 61 ILGDSNCILR ERDEFSRS- >C08HBa0217O07.1-_PGL-3_AGS-1_PPS_3 (3345 3151) (frame '2'; 192 bp, 64 residues) 1 GGGIRKVPVQ LVNLVLCLQD VLLTCIGGSV NNSLFLFEEQ SLIFALWILL NQMTFNSLLM 61 LVCV- PGL 4 (+ strand): 12877 18293 AGS-1 (12877 13080,14984 15087,15189 15264,17353 17436,17514 18293) SCR (e 1.000 d 0.941 a 0.760,e 1.000 d 0.859 a 0.971,e 1.000 d 0.856 a 0.965,e 1.000 d 0.934 a 0.988,e 0.994) Exon 1 12877 13080 ( 204 n); score: 1.000 Intron 1 13081 14983 (1903 n); Pd: 0.941 Pa: 0.760 Exon 2 14984 15087 ( 104 n); score: 1.000 Intron 2 15088 15188 ( 101 n); Pd: 0.859 Pa: 0.971 Exon 3 15189 15264 ( 76 n); score: 1.000 Intron 3 15265 17352 (2088 n); Pd: 0.856 Pa: 0.965 Exon 4 17353 17436 ( 84 n); score: 1.000 Intron 4 17437 17513 ( 77 n); Pd: 0.934 Pa: 0.988 Exon 5 17514 18293 ( 780 n); score: 0.994 PGS (12877 13080,14984 15087,15189 15264,17353 17436,17514 18293) SGN-U318998+ 3-phase translation of AGS-1 (+strand): . . . . . . 12877 GGCGTCAATTTGGAAAAAGTTCACCCGTCACCACCGTCAACGCCGTTAATTCTCGCCGGC G V N L E K V H P S P P S T P L I L A G A S I W K K F T R H H R Q R R - F S P A R Q F G K S S P V T T V N A V N S R R . . . . . . 12937 TCTTCATTCTCTAAACTCCGTCAACCCTCAATTCTTCCCCATCTCCAAAATCCTCATAAA S S F S K L R Q P S I L P H L Q N P H K L H S L N S V N P Q F F P I S K I L I N L F I L - T P S T L N S S P S P K S S - . . . . . . 12997 TCCTCTCTTCTCTCTACTCCTTTGCATTTCATTCTCTCCAACATCTGATTTTTTCCGATC S S L L S T P L H F I L S N I - F F P I P L F S L L L C I S F S P T S D F F R S I L S S L Y S F A F H S L Q H L I F S D . . . : . . . 13057 TCTCATCGAACTGCTGCGATGTCG : GTGAAAACTGTTAAAGTTAGCAATGTCTCCTTAGGG S H R T A A M S : V K T V K V S N V S L G L I E L L R C R : - K L L K L A M S P - G L S S N C C D V : G E N C - S - Q C L L R . . . . . . 15020 GCATCAGAGCGTGATATCAAGGAATTCTTTTCTTTCTCTGGTGATATTGAATATGTTGAG A S E R D I K E F F S F S G D I E Y V E H Q S V I S R N S F L S L V I L N M L R G I R A - Y Q G I L F F L W - Y - I C - . : . . . . . 15080 ATGCAAAG : TGATACTGAACGAAGTCAAATAGCATATGTTACGTTCAATGATTCTCAGGGA M Q S : D T E R S Q I A Y V T F N D S Q G C K : V I L N E V K - H M L R S M I L R E D A K : - Y - T K S N S I C Y V Q - F S G . . . : . . . 15241 GCTGACACTGCAGTTCTTCTTTCG : GGTGCAACAATAGTTGATATGTCTGTTACGGTGACT A D T A V L L S : G A T I V D M S V T V T L T L Q F F F R : V Q Q - L I C L L R - L S - H C S S S F : G C N N S - Y V C Y G D . . . . . : . 17389 TTGGATCCAGTTTACCAGCTTCCTCCTACTGCTTTTACAGCATCAGTG : CCAACTGGAAAG L D P V Y Q L P P T A F T A S V : P T G K W I Q F T S F L L L L L Q H Q C : Q L E R F G S S L P A S S Y C F Y S I S : A N W K . . . . . . 17526 AAAAGTGCTGGTAATTCAGAATCCGCTTTTCAGAAAGCAGAAGACGTTGTTAGCAGCATG K S A G N S E S A F Q K A E D V V S S M K V L V I Q N P L F R K Q K T L L A A C E K C W - F R I R F S E S R R R C - Q H . . . . . . 17586 CTCGCAAAAGGTTATATCTTAGGTAAAGATGCTGTAGGGAAAGCAAAGTCTTTTGATGAG L A K G Y I L G K D A V G K A K S F D E S Q K V I S - V K M L - G K Q S L L M R A R K R L Y L R - R C C R E S K V F - - . . . . . . 17646 AAACACCAGTTGACCTCAACTGCAACTGCTAAAGTGGCCTCTCTCGACAAAAAAATTGGT K H Q L T S T A T A K V A S L D K K I G N T S - P Q L Q L L K W P L S T K K L V E T P V D L N C N C - S G L S R Q K N W . . . . . . 17706 CTCACTGAAAAGATAAGCATTGGTACTTCCATTGTTAATGACAAAGTTCGAGAGGTGGAT L T E K I S I G T S I V N D K V R E V D S L K R - A L V L P L L M T K F E R W I S H - K D K H W Y F H C - - Q S S R G G . . . . . . 17766 CAGAAACTCCAAGTTTCTGATAAAGCAAAATCAGCACTTTCTGCAGCTGAGCAAACAGTT Q K L Q V S D K A K S A L S A A E Q T V R N S K F L I K Q N Q H F L Q L S K Q L S E T P S F - - S K I S T F C S - A N S . . . . . . 17826 AGTAGCGCTGGATCAGCCATTATGAAAAATCGGTATGTTCTGACTGGGTCCACTTGGGTA S S A G S A I M K N R Y V L T G S T W V V A L D Q P L - K I G M F - L G P L G - - - R W I S H Y E K S V C S D W V H L G . . . . . . 17886 GCCGGCGCTTTTAGTAAGGTTGCTAAGGCAGCAGGGGAAGTAGGCCAGATGACAAAAGAG A G A F S K V A K A A G E V G Q M T K E P A L L V R L L R Q Q G K - A R - Q K R S R R F - - G C - G S R G S R P D D K R . . . . . . 17946 AAAGTGGGAATGACAGAGGATGAGCAAAGGCAGAAAATGGTTACTGATTTCGCACAGGTT K V G M T E D E Q R Q K M V T D F A Q V K W E - Q R M S K G R K W L L I S H R F E S G N D R G - A K A E N G Y - F R T G . . . . . . 18006 CATTTATCTGAGTCTCCGAAAGCTTCCGAGTTCACAGAACATCAGCCAGCCAAGCCTGCA H L S E S P K A S E F T E H Q P A K P A I Y L S L R K L P S S Q N I S Q P S L H S F I - V S E S F R V H R T S A S Q A C . . . . . . 18066 CCAGTACAAGGTTTAGTCCTTTGAGTTGAAGGCCCTTGTAAGAACTATCTTTGAAGACTT P V Q G L V L - V E G P C K N Y L - R L Q Y K V - S F E L K A L V R T I F E D F T S T R F S P L S - R P L - E L S L K T . . . . . . 18126 CCACCTCGTACTACATTTCATTTTGCAATCCACTGTTTTATAAAGAAATGGCAAATGTAA P P R T T F H F A I H C F I K K W Q M - H L V L H F I L Q S T V L - R N G K C K S T S Y Y I S F C N P L F Y K E M A N V . . . . . . 18186 AACAGTATGTCCAATACAAATATTTAGATGATTTATTTTGTATTTGTGCTTTTTACTGCT N S M S N T N I - M I Y F V F V L F T A T V C P I Q I F R - F I L Y L C F L L L K Q Y V Q Y K Y L D D L F C I C A F Y C . . . . . 18246 ACAGAATTTGATTGAATTGAACATCAATGGTATTTCCAAGTAGATAAA T E F D - I E H Q W Y F Q V D K Q N L I E L N I N G I S K - I Y R I - L N - T S M V F P S R - Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1+_PGL-4_AGS-1_PPS_1 (13045 13080,14984 15087,15189 15264,17353 17436,17514 18089) (frame '1'; 873 bp, 291 residues) 1 FFPISHRTAA MSVKTVKVSN VSLGASERDI KEFFSFSGDI EYVEMQSDTE RSQIAYVTFN 61 DSQGADTAVL LSGATIVDMS VTVTLDPVYQ LPPTAFTASV PTGKKSAGNS ESAFQKAEDV 121 VSSMLAKGYI LGKDAVGKAK SFDEKHQLTS TATAKVASLD KKIGLTEKIS IGTSIVNDKV 181 REVDQKLQVS DKAKSALSAA EQTVSSAGSA IMKNRYVLTG STWVAGAFSK VAKAAGEVGQ 241 MTKEKVGMTE DEQRQKMVTD FAQVHLSESP KASEFTEHQP AKPAPVQGLV L- PGL 5 (- strand): 25816 24571 AGS-1 (25816 25385,25310 25266,25056 24571) SCR (e 1.000 d 0.886 a 0.976,e 1.000 d 0.787 a 0.996,e 0.988) Exon 1 25816 25385 ( 432 n); score: 1.000 Intron 1 25384 25311 ( 74 n); Pd: 0.886 Pa: 0.976 Exon 2 25310 25266 ( 45 n); score: 1.000 Intron 2 25265 25057 ( 209 n); Pd: 0.787 Pa: 0.996 Exon 3 25056 24571 ( 486 n); score: 0.988 PGS (25816 25385,25310 25266,25056 24571) SGN-U329136+ 3-phase translation of AGS-1 (-strand): . . . . . . 25816 ATTTGCGAGTAAATCCCCAGACTACTAAATAACCTCAACAGCTACCAAACACTCGCTTCG I C E - I P R L L N N L N S Y Q T L A S F A S K S P D Y - I T S T A T K H S L R L R V N P Q T T K - P Q Q L P N T R F . . . . . . 25756 CGCACTCTTTCTCAATCGCCGTTTCTCTCTCATTCTCGAATCTCCAGAATCCGGCCAACA R T L S Q S P F L S H S R I S R I R P T A L F L N R R F S L I L E S P E S G Q H A H S F S I A V S L S F S N L Q N P A N . . . . . . 25696 TGCATAGACAATCACTCGGCTCATCGGGATCGAAGTTACACCTTGCACATGGAGTCGTCC C I D N H S A H R D R S Y T L H M E S S A - T I T R L I G I E V T P C T W S R P M H R Q S L G S S G S K L H L A H G V V . . . . . . 25636 TCGTCGGCGGCAGCAGGGACGAATCCGCCGTCGTGACGGCGGCAGAGTCTCAGAAGATAA S S A A A G T N P P S - R R Q S L R R - R R R Q Q G R I R R R D G G R V S E D N L V G G S R D E S A V V T A A E S Q K I . . . . . . 25576 TGGCGAAGGACCAAGCCTCTCCGTCGTCACTCTCTAACAACTACGATGAAGGAGAAGAAC W R R T K P L R R H S L T T T M K E K N G E G P S L S V V T L - Q L R - R R R T M A K D Q A S P S S L S N N Y D E G E E . . . . . . 25516 AAGTACGCAAGTCAATCAAGGCTCTTAACAAGTCATTGTCTAGAGCTGAGAAGTACATTC K Y A S Q S R L L T S H C L E L R S T F S T Q V N Q G S - Q V I V - S - E V H S Q V R K S I K A L N K S L S R A E K Y I . . . . . . 25456 ACCTCATTCCTGTTCTCACATTTCTCTGCTTCTTCATCCTCTATCTATTCTCTCACAGTC T S F L F S H F S A S S S S I Y S L T V P H S C S H I S L L L H P L S I L S Q S H L I P V L T F L C F F I L Y L F S H S . . : . . . . : 25396 CGTCTGATAAAG : ATTTAGCTCAATTTCAAGGATTTGAAGGCTTCGCTAAGCGTATAG : AAT R L I K : I - L N F K D L K A S L S V - : N V - - R : F S S I S R I - R L R - A Y R : I P S D K : D L A Q F Q G F E G F A K R I : E . . . . . . 25053 CAGCAAATATTGACGACGAGTTGCAGAGAGTATTAGAAACTAAAAAACCGGAAGTTTTAG Q Q I L T T S C R E Y - K L K N R K F - S K Y - R R V A E S I R N - K T G S F S S A N I D D E L Q R V L E T K K P E V L . . . . . . 24993 CGATCCGAAGCGTAAGGAACTTGCAAGAGATTGATAGACAGGATTCAAATCATCGGCGCC R S E A - G T C K R L I D R I Q I I G A D P K R K E L A R D - - T G F K S S A P A I R S V R N L Q E I D R Q D S N H R R . . . . . . 24933 ACCGAAAACTCGCCGATTTTTAAGCCGCTGTTTGTGCTTCTTACTCTTCTTTATTCTCCG T E N S P I F K P L F V L L T L L Y S P P K T R R F L S R C L C F L L F F I L R H R K L A D F - A A V C A S Y S S L F S . . . . . . 24873 TATATTACTCAACCACCTATTCATGGCGAATTCACCTTCGGCGGAGCTTCTCTCACGTGC Y I T Q P P I H G E F T F G G A S L T C I L L N H L F M A N S P S A E L L S R A V Y Y S T T Y S W R I H L R R S F S H V . . . . . . 24813 GTAACTCGTTTCCTTGCCAATTTTACCGTTTCATTATTACCACGCCTGTGTGCATGTGAA V T R F L A N F T V S L L P R L C A C E - L V S L P I L P F H Y Y H A C V H V N R N S F P C Q F Y R F I I T T P V C M - . . . . . . 24753 TTTCTTATACTAGTAATTTTGGCTGTTTTGAACAAAATCAAATTTACCGGTGAAGTACTC F L I L V I L A V L N K I K F T G E V L F L Y - - F W L F - T K S N L P V K Y S I S Y T S N F G C F E Q N Q I Y R - S T . . . . . . 24693 TGTAAATATTTTACTTTACTATATATGATGTACCAAAAAAGCAAAATATTCACTTCCAAG C K Y F T L L Y M M Y Q K S K I F T S K V N I L L Y Y I - C T K K A K Y S L P S L - I F Y F T I Y D V P K K Q N I H F Q . . . . . . 24633 TTTTGGATACGTTAAATGGAAATTTCTGAGCTGGAGCTGGAAACTAGAAAGGAAAATATC F W I R - M E I S E L E L E T R K E N I F G Y V K W K F L S W S W K L E R K I S V L D T L N G N F - A G A G N - K G K Y . 24573 AAA K Q Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1-_PGL-5_AGS-1_PPS_1 (25784 25385,25310 25266,25056 24911) (frame '0'; 588 bp, 196 residues) 1 PQQLPNTRFA HSFSIAVSLS FSNLQNPANM HRQSLGSSGS KLHLAHGVVL VGGSRDESAV 61 VTAAESQKIM AKDQASPSSL SNNYDEGEEQ VRKSIKALNK SLSRAEKYIH LIPVLTFLCF 121 FILYLFSHSP SDKDLAQFQG FEGFAKRIES ANIDDELQRV LETKKPEVLA IRSVRNLQEI 181 DRQDSNHRRH RKLADF- PGL 6 (+ strand): 28571 32211 AGS-1 (28571 28841,31477 32211) SCR (e 0.991 d 0.994 a 1.000,e 1.000) Exon 1 28571 28841 ( 271 n); score: 0.991 Intron 1 28842 31476 (2635 n); Pd: 0.994 Pa: 1.000 Exon 2 31477 32211 ( 735 n); score: 1.000 PGS (28571 28841,31477 32211) SGN-U321737+ 3-phase translation of AGS-1 (+strand): . . . . . . 28571 AGGGACTACTTTAACTTGCCTTGTTGAAAACGTAAACGGAAACGCCATATTTTCTCATAA R D Y F N L P C - K R K R K R H I F S - G T T L T C L V E N V N G N A I F S H K G L L - L A L L K T - T E T P Y F L I . . . . . . 28631 AGCTTGAAGAAGAAAAACAGATTTTTTTTTTTGGCCTGAAAAAGAGACTGATAGAGGAAG S L K K K N R F F F L A - K R D - - R K A - R R K T D F F F W P E K E T D R G R K L E E E K Q I F F F G L K K R L I E E . . . . . . 28691 AAGAAAGTTCTAGAAAGAAAATGGTTCTATGGGAGATCACATTGGGAACGGCGTATTTTT K K V L E R K W F Y G R S H W E R R I F R K F - K E N G S M G D H I G N G V F F E E S S R K K M V L W E I T L G T A Y F . . . . . . 28751 TGGGTCTGAAGCGAACTTACAGGCTGTTCTTGAGGATTCAGCGTAAGCTCATTAGTCCAA W V - S E L T G C S - G F S V S S L V Q G S E A N L Q A V L E D S A - A H - S K L G L K R T Y R L F L R I Q R K L I S P . . . . : . . 28811 AGTATCCTAGGCTTCGTGATTTCGCTCAAAG : GCGAACACGAGCTGCATTTGATATGGCAC S I L G F V I S L K : G E H E L H L I W H V S - A S - F R S K : A N T S C I - Y G T K Y P R L R D F A Q R : R T R A A F D M A . . . . . . 31506 TGATCGTTCATCGCAAGGTACAGGAAAGAGATATTGAAGCTGGTCGGAACCTTGGTAATT - S F I A R Y R K E I L K L V G T L V I D R S S Q G T G K R Y - S W S E P W - L L I V H R K V Q E R D I E A G R N L G N . . . . . . 31566 GGATCCTTCGATGGCTTGACAAAATGAAGCCATCTGCTAATATTCGAGGCTCAGATAACA G S F D G L T K - S H L L I F E A Q I T D P S M A - Q N E A I C - Y S R L R - H W I L R W L D K M K P S A N I R G S D N . . . . . . 31626 TTGCAATTACCAGTGCAACTAAGCAGCTTAGGAACTCATCTCACTCCCCCAAGCCTGAAG L Q L P V Q L S S L G T H L T P P S L K C N Y Q C N - A A - E L I S L P Q A - S I A I T S A T K Q L R N S S H S P K P E . . . . . . 31686 CTTTCCAGAAATACGGTGCTGGCAAAGACAAGGAATCCAGCAGCAGGCATCTGTTTACCT L S R N T V L A K T R N P A A G I C L P F P E I R C W Q R Q G I Q Q Q A S V Y L A F Q K Y G A G K D K E S S S R H L F T . . . . . . 31746 CAGCTAGTAACACATGGCAGAAAGCATACCCGACCATTTCCATGATGATGAAACCAAGAA Q L V T H G R K H T R P F P - - - N Q E S - - H M A E S I P D H F H D D E T K K S A S N T W Q K A Y P T I S M M M K P R . . . . . . 31806 GCCCAGCTGGAACAAATATCCAATACAGGCAGTTAAACACTGTTTCGCCTACTAGTTTCA A Q L E Q I S N T G S - T L F R L L V S P S W N K Y P I Q A V K H C F A Y - F Q S P A G T N I Q Y R Q L N T V S P T S F . . . . . . 31866 AACCCATGAAACATGGTTTTGAGGGAGTGATCCGACCGGACATATTGCAGTGGATTCAAC N P - N M V L R E - S D R T Y C S G F N T H E T W F - G S D P T G H I A V D S T K P M K H G F E G V I R P D I L Q W I Q . . . . . . 31926 GAAGCTAATCCATTAACTACTCTGTTGCTGCCTTTTGGCTTTTACACTAGTGGACTTGAA E A N P L T T L L L P F G F Y T S G L E K L I H - L L C C C L L A F T L V D L N R S - S I N Y S V A A F W L L H - W T - . . . . . . 31986 CGTCTGCAGAAGGTGCATATACGGCACAAATAAAAGCTCACTAGATCAGTGCCGTCATGA R L Q K V H I R H K - K L T R S V P S - V C R R C I Y G T N K S S L D Q C R H E T S A E G A Y T A Q I K A H - I S A V M . . . . . . 32046 AAAAATTTTAGTCTCTGTTTTCTTTTGGCTGAACAGTCAATATTGTACATTCGTTTCAGA K N F S L C F L L A E Q S I L Y I R F R K I L V S V F F W L N S Q Y C T F V S D K K F - S L F S F G - T V N I V H S F Q . . . . . . 32106 TTCTGACCTGTGTGTACCTCAACCAACCTTGATTCTTCTGTTGTTTCTACTCTGTTGTAG F - P V C T S T N L D S S V V S T L L - S D L C V P Q P T L I L L L F L L C C S I L T C V Y L N Q P - F F C C F Y S V V . . . . . 32166 CCTCTAATGCAATAACAAATAGTATTGAAGTCAATTTTTTTTGTTA P L M Q - Q I V L K S I F F V L - C N N K - Y - S Q F F L L A S N A I T N S I E V N F F C Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1+_PGL-6_AGS-1_PPS_1 (28606 28841,31477 31933) (frame '0'; 690 bp, 230 residues) 1 TETPYFLIKL EEEKQIFFFG LKKRLIEEEE SSRKKMVLWE ITLGTAYFLG LKRTYRLFLR 61 IQRKLISPKY PRLRDFAQRR TRAAFDMALI VHRKVQERDI EAGRNLGNWI LRWLDKMKPS 121 ANIRGSDNIA ITSATKQLRN SSHSPKPEAF QKYGAGKDKE SSSRHLFTSA SNTWQKAYPT 181 ISMMMKPRSP AGTNIQYRQL NTVSPTSFKP MKHGFEGVIR PDILQWIQRS - >C08HBa0217O07.1+_PGL-6_AGS-1_PPS_2 (31942 32172) (frame '2'; 228 bp, 76 residues) 1 LLCCCLLAFT LVDLNVCRRC IYGTNKSSLD QCRHEKILVS VFFWLNSQYC TFVSDSDLCV 61 PQPTLILLLF LLCCSL- PGL 7 (+ strand): 35261 36132 AGS-1 (35261 36132) SCR (e 1.000) Exon 1 35261 36132 ( 872 n); score: 1.000 PGS (35261 35934) SGN-U337363+ PGS (35473 36132) SGN-U321387- 3-phase translation of AGS-1 (+strand): . . . . . . 35261 TTTTTTTTCTCTATAAAAAGAAGTAACTACCCTTTTATTATTTATCACAATCATTCAATA F F F S I K R S N Y P F I I Y H N H S I F F S L - K E V T T L L L F I T I I Q - F F L Y K K K - L P F Y Y L S Q S F N . . . . . . 35321 ACCAAAAAAAAAAAAAAAATTAGTGAGAAGAAAGTGAAAATATTATTATGGATCAGTGGA T K K K K K I S E K K V K I L L W I S G P K K K K K L V R R K - K Y Y Y G S V E N Q K K K K N - - E E S E N I I M D Q W . . . . . . 35381 AGAAGCTTTACGTTCTTGGCGCTGGCTCTTACGGCAAAGTTTATTATGCAGTGAAGATGG R S F T F L A L A L T A K F I M Q - R W E A L R S W R W L L R Q S L L C S E D G K K L Y V L G A G S Y G K V Y Y A V K M . . . . . . 35441 ATCCTGTTTCGTTATGTGTTTCTGTTGCTGCTGTTAAATGTGCTGATATGAACCGTTCTG I L F R Y V F L L L L L N V L I - T V L S C F V M C F C C C C - M C - Y E P F C D P V S L C V S V A A V K C A D M N R S . . . . . . 35501 TTTCACTCCAACGAGAAGCAGAAATCTTGACAACCCTCAAAGATTCACCTTACGTGGTTC F H S N E K Q K S - Q P S K I H L T W F F T P T R S R N L D N P Q R F T L R G S V S L Q R E A E I L T T L K D S P Y V V . . . . . . 35561 AGTTCATTGGATCAGACGTTAGCATCGATAACGGTAATGTCTCAACTTACAATCTGTTTC S S L D Q T L A S I T V M S Q L T I C F V H W I R R - H R - R - C L N L Q S V S Q F I G S D V S I D N G N V S T Y N L F . . . . . . 35621 TTGAGTATGCATATGGTGGATCGCTACACGATTTGATCATTAATTCGAAGATGAGAAGGA L S M H M V D R Y T I - S L I R R - E G - V C I W W I A T R F D H - F E D E K D L E Y A Y G G S L H D L I I N S K M R R . . . . . . 35681 TACGGATGTCTGAAGTGGAAGTAGGTTTCTATGCATATCAACTCTTAAAGGGTATTCAAC Y G C L K W K - V S M H I N S - R V F N T D V - S G S R F L C I S T L K G Y S T I R M S E V E V G F Y A Y Q L L K G I Q . . . . . . 35741 ACGTTCATGAGAAAGGGTGGGTTCATTGTGATATTAAACCTGCTAATGTTTTGATTTTCA T F M R K G G F I V I L N L L M F - F S R S - E R V G S L - Y - T C - C F D F Q H V H E K G W V H C D I K P A N V L I F . . . . . . 35801 ACAACGCTGAACGTGGTGGGATGCACAAGTTAAAGTTGGCTGACTTTGGATTGTCGTTGA T T L N V V G C T S - S W L T L D C R - Q R - T W W D A Q V K V G - L W I V V E N N A E R G G M H K L K L A D F G L S L . . . . . . 35861 GAGTTCCCGAAGGTGTGGCATATATGACTGGTGCTGCGATGAGCAACAGAGGGACTCTAC E F P K V W H I - L V L R - A T E G L Y S S R R C G I Y D W C C D E Q Q R D S T R V P E G V A Y M T G A A M S N R G T L . . . . . . 35921 CTTACGCGCCCCCAGAATCTTTGATCAGTGGTTTTCATGGCAGGAGTTATGATATATGGT L T R P Q N L - S V V F M A G V M I Y G L R A P R I F D Q W F S W Q E L - Y M V P Y A P P E S L I S G F H G R S Y D I W . . . . . . 35981 CGTTAGGGTGTACTGTGGCAGAGATGATGACTGGGTGTCGCGTTTGGATTTATCGCGACA R - G V L W Q R - - L G V A F G F I A T V R V Y C G R D D D W V S R L D L S R H S L G C T V A E M M T G C R V W I Y R D . . . . . . 36041 CTAAGGATTTGCAATGGAAGATTATGAATGAAGAACCAATGGTTCCTAGTGATGTTTCTG L R I C N G R L - M K N Q W F L V M F L - G F A M E D Y E - R T N G S - - C F - T K D L Q W K I M N E E P M V P S D V S . . . . 36101 AGATTGCCAGAGATTTTTTGTACAAGTGTTTG R L P E I F C T S V D C Q R F F V Q V F E I A R D F L Y K C L Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0217O07.1+_PGL-7_AGS-1_PPS_1 (35347 36132) (frame '0'; 786 bp, 262 residues) 1 EESENIIMDQ WKKLYVLGAG SYGKVYYAVK MDPVSLCVSV AAVKCADMNR SVSLQREAEI 61 LTTLKDSPYV VQFIGSDVSI DNGNVSTYNL FLEYAYGGSL HDLIINSKMR RIRMSEVEVG 121 FYAYQLLKGI QHVHEKGWVH CDIKPANVLI FNNAERGGMH KLKLADFGLS LRVPEGVAYM 181 TGAAMSNRGT LPYAPPESLI SGFHGRSYDI WSLGCTVAEM MTGCRVWIYR DTKDLQWKIM 241 NEEPMVPSDV SEIARDFLYK CL 3-phase translation of AGS-1 (-strand): . . . . . . 36132 CAAACACTTGTACAAAAAATCTCTGGCAATCTCAGAAACATCACTAGGAACCATTGGTTC Q T L V Q K I S G N L R N I T R N H W F K H L Y K K S L A I S E T S L G T I G S N T C T K N L W Q S Q K H H - E P L V . . . . . . 36072 TTCATTCATAATCTTCCATTGCAAATCCTTAGTGTCGCGATAAATCCAAACGCGACACCC F I H N L P L Q I L S V A I N P N A T P S F I I F H C K S L V S R - I Q T R H P L H S - S S I A N P - C R D K S K R D T . . . . . . 36012 AGTCATCATCTCTGCCACAGTACACCCTAACGACCATATATCATAACTCCTGCCATGAAA S H H L C H S T P - R P Y I I T P A M K V I I S A T V H P N D H I S - L L P - K Q S S S L P Q Y T L T T I Y H N S C H E . . . . . . 35952 ACCACTGATCAAAGATTCTGGGGGCGCGTAAGGTAGAGTCCCTCTGTTGCTCATCGCAGC T T D Q R F W G R V R - S P S V A H R S P L I K D S G G A - G R V P L L L I A A N H - S K I L G A R K V E S L C C S S Q . . . . . . 35892 ACCAGTCATATATGCCACACCTTCGGGAACTCTCAACGACAATCCAAAGTCAGCCAACTT T S H I C H T F G N S Q R Q S K V S Q L P V I Y A T P S G T L N D N P K S A N F H Q S Y M P H L R E L S T T I Q S Q P T . . . . . . 35832 TAACTTGTGCATCCCACCACGTTCAGCGTTGTTGAAAATCAAAACATTAGCAGGTTTAAT - L V H P T T F S V V E N Q N I S R F N N L C I P P R S A L L K I K T L A G L I L T C A S H H V Q R C - K S K H - Q V - . . . . . . 35772 ATCACAATGAACCCACCCTTTCTCATGAACGTGTTGAATACCCTTTAAGAGTTGATATGC I T M N P P F L M N V L N T L - E L I C S Q - T H P F S - T C - I P F K S - Y A Y H N E P T L S H E R V E Y P L R V D M . . . . . . 35712 ATAGAAACCTACTTCCACTTCAGACATCCGTATCCTTCTCATCTTCGAATTAATGATCAA I E T Y F H F R H P Y P S H L R I N D Q - K P T S T S D I R I L L I F E L M I K H R N L L P L Q T S V S F S S S N - - S . . . . . . 35652 ATCGTGTAGCGATCCACCATATGCATACTCAAGAAACAGATTGTAAGTTGAGACATTACC I V - R S T I C I L K K Q I V S - D I T S C S D P P Y A Y S R N R L - V E T L P N R V A I H H M H T Q E T D C K L R H Y . . . . . . 35592 GTTATCGATGCTAACGTCTGATCCAATGAACTGAACCACGTAAGGTGAATCTTTGAGGGT V I D A N V - S N E L N H V R - I F E G L S M L T S D P M N - T T - G E S L R V R Y R C - R L I Q - T E P R K V N L - G . . . . . . 35532 TGTCAAGATTTCTGCTTCTCGTTGGAGTGAAACAGAACGGTTCATATCAGCACATTTAAC C Q D F C F S L E - N R T V H I S T F N V K I S A S R W S E T E R F I S A H L T L S R F L L L V G V K Q N G S Y Q H I - . . . . . . 35472 AGCAGCAACAGAAACACATAACGAAACAGGATCCATCTTCACTGCATAATAAACTTTGCC S S N R N T - R N R I H L H C I I N F A A A T E T H N E T G S I F T A - - T L P Q Q Q Q K H I T K Q D P S S L H N K L C . . . . . . 35412 GTAAGAGCCAGCGCCAAGAACGTAAAGCTTCTTCCACTGATCCATAATAATATTTTCACT V R A S A K N V K L L P L I H N N I F T - E P A P R T - S F F H - S I I I F S L R K S Q R Q E R K A S S T D P - - Y F H . . . . . . 35352 TTCTTCTCACTAATTTTTTTTTTTTTTTTGGTTATTGAATGATTGTGATAAATAATAAAA F F S L I F F F F L V I E - L - - I I K S S H - F F F F F W L L N D C D K - - K F L L T N F F F F F G Y - M I V I N N K . . . . 35292 GGGTAGTTACTTCTTTTTATAGAGAAAAAAAA G - L L L F I E K K G S Y F F L - R K K R V V T S F Y R E K K Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Tue Aug 29 00:01:17 2006