GeneSeqer. Version of March 12, 2006. Date run: Tue Jul 25 11:16:41 2006 (Bayesian) Splice site model (species): Arabidopsis thaliana Fast search parameters: MinMatchLen 16, MinQualityHSP 30, MinQualityCHAIN 50. Total number of ESTs: 175665 Total sequence length: 93213537 Minimum sequence length: 89 Maximum sequence length: 1082 Length distribution (number of sequences of specified length): < 100: 1 < 200: 2188 < 300: 8544 < 400: 20465 < 500: 39499 < 600: 49432 < 700: 32872 < 800: 19308 < 900: 3155 < 1000: 193 >=1000: 8 Input file : /tmp/bac-submission-temp-aqJUN/C08HBa0251H04/C08HBa0251H04.seq.screen ________________________________________________________________________________ Sequence 1: C08HBa0251H04.1, from 1 to 158110, both strands analyzed. ... started at: Tue Jul 25 11:44:29 2006 EST library file: /tmp/cxgn-bacpublish-resources-qtsRaS/sgn_ests; matching gDNA +strand ... ... found all matches, elapsed seconds = 8 ... matches indexed, elapsed seconds = 9 HitsTableSize = 62 EST library file: /tmp/cxgn-bacpublish-resources-qtsRaS/sgn_ests; matching gDNA -strand ... ... found all matches, elapsed seconds = 8 ... matches indexed, elapsed seconds = 9 HitsTableSize = 87 ******************************************************************************** EST sequence 21 -strand 718 n (File: SGN-E373790-) 1 CATCACTCAA ACTTGCGTCG CGGTGCAGCT GGATATATCT TTAATACTGT TCTTGCTCCT 61 GCTTTTGGGC TGCCATTGAA TCCACAAGCA GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA 121 TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC CAGAGAAAAG GACGGTTTTT GCTTGGGAGT 181 GGCCAACCTT CAATTGCAGA TCTTAGCTTA GTGTGTGAGA TTATGGAACT TGAGATTTTG 241 GATGAGAAGG ATCGTGAGCG GATAATAGGC CCATACAAGA GAGTTTTGAA GTGGATTGAT 301 GACACAAAGA ATGCCATGGA ACCTCATTTC CAAGAGGTAC ATGTGATCCT CTTCAAAGCT 361 AAAGAGAAGT TCCACAAGCA AAGACATGCT GTAGGAAGTA GCATTCCTCA ATCGAGCAGA 421 AAACCTGACT TGCACTCTAA GATGTGAGAA ACCAACAAGG ATCCAGGTGC TGCATTGTGC 481 TCGTAGTATC ACCGTGCACC ATGGCAGATA AACAGTGTGT ATGCAAAAGT TGATACAGGA 541 AGGAGAGTTC TGATCCAAGC TTTCCTTACT CATTTTCTCC ATGTATATGT ACTTTATAAG 601 TTTCTATCAG TTGTTAATGT AGTCCACAAC ATATGTAATC GAACAAACTG AGAGTCCCTT 661 GCTCTTGTGC TTGTAACTTC AGAGCTTATG AACACATTTT TGTCTAAAAA AAAAAAAA Predicted gene structure (within gDNA segment 10981 to 3294): Exon 1 4686 4659 ( 28 n); cDNA 1 28 ( 28 n); score: 1.000 Intron 1 4658 4115 ( 544 n); Pd: 0.850 (s: 0), Pa: 0.096 (s: 1.00) Exon 2 4114 3909 ( 206 n); cDNA 29 234 ( 206 n); score: 1.000 Intron 2 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 3783 3304 ( 480 n); cDNA 235 714 ( 480 n); score: 0.992 MATCH C08HBa0251H04.1- SGN-E373790- 0.994 714 0.994 C PGS_C08HBa0251H04.1-_SGN-E373790- (4686 4659,4114 3909,3783 3304) Alignment (genomic DNA sequence = upper lines): CATCACTCAA ACTTGCGTCG CGGTGCAGGT GCTTATATCA GATATTGAGA TCATTTCAGT 4627 |||||||||| |||||||||| |||||||| CATCACTCAA ACTTGCGTCG CGGTGCAG.. .......... .......... .......... 28 TTCCGGTTGA ATTATGATTA CTTTACGTTG ACCTTACTTG AGTTAATTTA GTGGTGAATG 4567 .......... .......... .......... .......... .......... .......... 28 CTGGGATATG CAAGAACTTG GGCAAAAAGA TGCATACAGG CTACAGTTAA GGCAATGACT 4507 .......... .......... .......... .......... .......... .......... 28 GGATTAAAAT TTTATCCCAA ATAAGATTAA TCAAGGAAAT CTGTTTGTAT GATCAGTTTT 4447 .......... .......... .......... .......... .......... .......... 28 AAAAAAGAGT ATCTGCTTGT CATGATGCTC TTTGCTAAGA TTCTCGCTAC TAACCTGATA 4387 .......... .......... .......... .......... .......... .......... 28 CAAGAGAGTT GCTGCAAGCC TACAAGTTTC TTAGTCTATC TTTAGGACTT GATTCCTCTA 4327 .......... .......... .......... .......... .......... .......... 28 ATTGAAACAC CTGACCTTTC TCAAACCTCT AATTGTGAGT CTAGCTACTG CATGATGGTG 4267 .......... .......... .......... .......... .......... .......... 28 ACTTCTTTTC TTCAACAATA ACAGTGACTA AAACCACTAG GATAGGTAGT CTTTTCACCA 4207 .......... .......... .......... .......... .......... .......... 28 ATGTTCTATA ATTACTACTT CTTGTTGATA ACTTATTATG CCACTCTTTA TCTGTTTCCA 4147 .......... .......... .......... .......... .......... .......... 28 ACAGTTTTCT GAAGCAGTAC TTATGTTTTC AGCTGGATAT ATCTTTAATA CTGTTCTTGC 4087 |||||||| |||||||||| |||||||||| .......... .......... .......... ..CTGGATAT ATCTTTAATA CTGTTCTTGC 56 TCCTGCTTTT GGGCTGCCAT TGAATCCACA AGCAGCAGCA GAAGCTGAGA AAGTCCTTTT 4027 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCTGCTTTT GGGCTGCCAT TGAATCCACA AGCAGCAGCA GAAGCTGAGA AAGTCCTTTT 116 GGCATCTCTT GCAAAAGTCG AGTCTGTTTG GCTCCAGAGA AAAGGACGGT TTTTGCTTGG 3967 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCATCTCTT GCAAAAGTCG AGTCTGTTTG GCTCCAGAGA AAAGGACGGT TTTTGCTTGG 176 GAGTGGCCAA CCTTCAATTG CAGATCTTAG CTTAGTGTGT GAGATTATGG AACTTGAGGT 3907 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| GAGTGGCCAA CCTTCAATTG CAGATCTTAG CTTAGTGTGT GAGATTATGG AACTTGAG.. 234 AAAGAGATTT TCCCTTTTCC TGCTGTTTCT TTACATCTTT TGATCCAGAA CATAAATTAC 3847 .......... .......... .......... .......... .......... .......... 234 CAAGAGGAGT CTTGTTGAAA TTTCTTTTAT AATGAATTTG GGTTTTGGAT GTGATGATTT 3787 .......... .......... .......... .......... .......... .......... 234 TAGATTTTGG ATGAGAAGGA TCGTGAGCGG ATAATAGGCC CATACAAGAG AGTTTTGAAG 3727 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...ATTTTGG ATGAGAAGGA TCGTGAGCGG ATAATAGGCC CATACAAGAG AGTTTTGAAG 291 TGGATTGATG ACACAAAGAA TGCCATGGAA CCTCATTTCC AAGAGGTACA TGTGATCCTC 3667 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGATTGATG ACACAAAGAA TGCCATGGAA CCTCATTTCC AAGAGGTACA TGTGATCCTC 351 TTCAAAGCTA AAGAGAAGTT CCACAAGCAA AGACATGCTG TAGGAAGTAG CATTCCTCAA 3607 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCAAAGCTA AAGAGAAGTT CCACAAGCAA AGACATGCTG TAGGAAGTAG CATTCCTCAA 411 TCGAGCAGAA AACCTGACTT GCACTCTAAG ATGTGAGAAA CCAACAAGGA TCCAGGTGCT 3547 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGAGCAGAA AACCTGACTT GCACTCTAAG ATGTGAGAAA CCAACAAGGA TCCAGGTGCT 471 GCATTGTGCT CGTAGTATCA CCGTGCACCA TGGCAGATAA ACAGTGTGTA TGCAAAAGTT 3487 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATTGTGCT CGTAGTATCA CCGTGCACCA TGGCAGATAA ACAGTGTGTA TGCAAAAGTT 531 GATACAGGAA GGAGAGTTCT GATCCAAGCT TTCCTTACTC ATTTTCTCCA TGTATATGTA 3427 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATACAGGAA GGAGAGTTCT GATCCAAGCT TTCCTTACTC ATTTTCTCCA TGTATATGTA 591 CTTTATAAGT TTCTATCAGT TGTTAATGTA GTCCACAACA TATGTAATCG AACAAACTGA 3367 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTTATAAGT TTCTATCAGT TGTTAATGTA GTCCACAACA TATGTAATCG AACAAACTGA 651 GAGTCCCTTG CTCTTGTGCT TGTAACTTCA GAGCTTATGA ACACATTTTT GTCTACAATG 3307 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| || GAGTCCCTTG CTCTTGTGCT TGTAACTTCA GAGCTTATGA ACACATTTTT GTCTAAAAAA 711 TAA 3304 || AAA 714 hqPGS_C08HBa0251H04.1-_SGN-E373790- (4686 4659,4114 3909,3783 3304) ******************************************************************************** EST sequence 73 +strand 660 n (File: SGN-E210728+) 1 TTTGAGGAGA TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAGAA 61 ATTAATCCCA TGAAGCAAGT ACCAGCTATA ATTGATGGAA GATTTAAGCT TTTCGAAAGT 121 CATGCAATTC TTAGATATCT GGCTTGTGCA TTTCCAGGAA TAGCAGATCA TTGGTACCCT 181 GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 241 CGTCGCGGTG CAGCTGGATA TATCTTTAAT ACTGTTCTTG CTCCTGCTTT TGGGCTGCCA 301 TTGAATCCAC AAGCAGCAGC AGAAGCTGAG AAAGTCCTTT TGGCATCTCT TGCAAAAGTC 361 GAGTCTGTTT GGCTCCAGAG AAAAGGACGG TTTTTGCTTG GGAGTGGCCA ACCTTCAATT 421 GCAGATCTTA GCTTAGTGTG TGAGATTATG GAACTTGAGA TTTTGGATGA GAAGGATCGT 481 GAGCGGATAA TAGGCCCATA CAAGAGAGTT TTGAAGTGGA TTGATGACAC AAAGAATGCC 541 ATGGAACCTC ATTTCCAAGA GGTACATGTG ATCCTCTTCA AAGCTAAAGA GAAGTTCCAC 601 AAGCAAAGAC ATGCTGTAGG AAGTAGCATT CCTCAATCGA GCAGAAAACC TGACTTGCAC Predicted gene structure (within gDNA segment 8564 to 1403): Exon 1 6212 6155 ( 58 n); cDNA 1 58 ( 58 n); score: 1.000 Intron 1 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 2 6062 6002 ( 61 n); cDNA 59 119 ( 61 n); score: 1.000 Intron 2 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 3 5651 5598 ( 54 n); cDNA 120 173 ( 54 n); score: 1.000 Intron 3 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 4 4738 4659 ( 80 n); cDNA 174 253 ( 80 n); score: 1.000 Intron 4 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 5 4114 3909 ( 206 n); cDNA 254 459 ( 206 n); score: 1.000 Intron 5 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 6 3783 3583 ( 201 n); cDNA 460 660 ( 201 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E210728+ 1.000 660 1.000 C PGS_C08HBa0251H04.1-_SGN-E210728+ (6212 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3583) Alignment (genomic DNA sequence = upper lines): TTTGAGGAGA TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAGGT 6153 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTTGAGGAGA TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAG.. 58 TTGTCTTTTT TATTTACTCC ACTTTTCGCT AGATATTATT TATTTCTTAG TTTCAAGATA 6093 .......... .......... .......... .......... .......... .......... 58 ATTTCAAAAG TTTTCTGACT TCATCTTCAG AAATTAATCC CATGAAGCAA GTACCAGCTA 6033 |||||||||| |||||||||| |||||||||| .......... .......... .......... AAATTAATCC CATGAAGCAA GTACCAGCTA 88 TAATTGATGG AAGATTTAAG CTTTTCGAAA GGTAATATTC AGCTTGAACT GAACATTTTA 5973 |||||||||| |||||||||| |||||||||| | TAATTGATGG AAGATTTAAG CTTTTCGAAA G......... .......... .......... 119 GCATTTTCCA GTATCACAAC TTTGTTAACG TCTATTATTC GCTGTATATG TTGCATACAT 5913 .......... .......... .......... .......... .......... .......... 119 CCTAAAGCTT ATGATTTAGT CTTTCATTCT CGTAGAAGTC ATGTTTGTAG CGTTCACCTA 5853 .......... .......... .......... .......... .......... .......... 119 ATCACCTGTG CATATATACT GCAAGAAGTG AAAAAATTTG TAAAAATTTC TATGTTTTGT 5793 .......... .......... .......... .......... .......... .......... 119 GTACACCATG TGGGCACAAG TAACTACTGT TGAAAATGCC TGTTGAGACG GGACAATTTT 5733 .......... .......... .......... .......... .......... .......... 119 ATCTTTTTCT GAGTTTCACA TAAAGTATTT TATAACTTGG ATAATGTTTA AAATTAACTA 5673 .......... .......... .......... .......... .......... .......... 119 ACTGATTATA TGTCCTCGCA GTCATGCAAT TCTTAGATAT CTGGCTTGTG CATTTCCAGG 5613 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .TCATGCAAT TCTTAGATAT CTGGCTTGTG CATTTCCAGG 158 AATAGCAGAT CATTGGTAAG TATCGACTTA TTTTGAGTAT ATACTGTAGT CAAATGCTTT 5553 |||||||||| ||||| AATAGCAGAT CATTG..... .......... .......... .......... .......... 173 GTATCATGGA AATAAGTACT CTTTAAGCGC TTTTTCTAGA CATAGGATCC AACCCGGACA 5493 .......... .......... .......... .......... .......... .......... 173 GATCAGGGAT ATAAACTTGA TGGGTTCAAC TTTTATATTC TCACTGCTGG TGCAATCACG 5433 .......... .......... .......... .......... .......... .......... 173 TGTATACCTA AGTTCCATGT CAAAATGTTG GGTTAAGCAG AACCCATATT GCATCTGCTG 5373 .......... .......... .......... .......... .......... .......... 173 CTGAACATGG CAATCGGCAT TACGAATGGA ATTATTCTGC AGTTGGCATC TGTGTGAATT 5313 .......... .......... .......... .......... .......... .......... 173 TAATGCCACA TGTACCTGTT ATTTGATTGA TAATTGTTTG TTGTTTTTCT AACTTGGAGT 5253 .......... .......... .......... .......... .......... .......... 173 TCAACTAGTA GAGTTTTGAT AAGAAACGCC ATCGGGTTTT GCTAAGAAAC CCGTCATTTG 5193 .......... .......... .......... .......... .......... .......... 173 TACCAAAACC GAGAATAACA TGACATGTGT CTTCTGCATA CACTTGTCAC CTAACTATTG 5133 .......... .......... .......... .......... .......... .......... 173 TTAATTAATG TGCATTTTCA TCTCATTTAT TACTTAAGAA TGGTAAAATA GTGAACGAAC 5073 .......... .......... .......... .......... .......... .......... 173 CAAAGGAGAA CTCCCAACAG AAAAGAAGAA CAAGTAACAT ATTAGCAGAT TGGAAAATAC 5013 .......... .......... .......... .......... .......... .......... 173 CAGCTCAACA AATGAAGTAT CAGCACCAAA TAACAAACAA GCCCTATCAA AATTTGAGAA 4953 .......... .......... .......... .......... .......... .......... 173 GAGATGACTA TTGAACACAG AAGTCATCCT TTGGAATCTC TGTACTATAC CAACTTATCC 4893 .......... .......... .......... .......... .......... .......... 173 GAAAAAAGAA AAAAAAAGTA CATCTATGGT GCCTATGAAC AATGCTGCTC TTCATTCTTA 4833 .......... .......... .......... .......... .......... .......... 173 GTGATAGACT AATGTACACA CTCTTAGGTT GTATAAATTC TTATCCTGGT TAGTGTTTGA 4773 .......... .......... .......... .......... .......... .......... 173 TTTAAATCCT CTTTCTTACA CTTTCCTATT GAAGGTACCC TGCTGACTTA TACAAAAGAG 4713 |||||| |||||||||| |||||||||| .......... .......... .......... ....GTACCC TGCTGACTTA TACAAAAGAG 199 CAAAGGTAGA CTCTGTGTTG GACTGGCATC ACTCAAACTT GCGTCGCGGT GCAGGTGCTT 4653 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| CAAAGGTAGA CTCTGTGTTG GACTGGCATC ACTCAAACTT GCGTCGCGGT GCAG...... 253 ATATCAGATA TTGAGATCAT TTCAGTTTCC GGTTGAATTA TGATTACTTT ACGTTGACCT 4593 .......... .......... .......... .......... .......... .......... 253 TACTTGAGTT AATTTAGTGG TGAATGCTGG GATATGCAAG AACTTGGGCA AAAAGATGCA 4533 .......... .......... .......... .......... .......... .......... 253 TACAGGCTAC AGTTAAGGCA ATGACTGGAT TAAAATTTTA TCCCAAATAA GATTAATCAA 4473 .......... .......... .......... .......... .......... .......... 253 GGAAATCTGT TTGTATGATC AGTTTTAAAA AAGAGTATCT GCTTGTCATG ATGCTCTTTG 4413 .......... .......... .......... .......... .......... .......... 253 CTAAGATTCT CGCTACTAAC CTGATACAAG AGAGTTGCTG CAAGCCTACA AGTTTCTTAG 4353 .......... .......... .......... .......... .......... .......... 253 TCTATCTTTA GGACTTGATT CCTCTAATTG AAACACCTGA CCTTTCTCAA ACCTCTAATT 4293 .......... .......... .......... .......... .......... .......... 253 GTGAGTCTAG CTACTGCATG ATGGTGACTT CTTTTCTTCA ACAATAACAG TGACTAAAAC 4233 .......... .......... .......... .......... .......... .......... 253 CACTAGGATA GGTAGTCTTT TCACCAATGT TCTATAATTA CTACTTCTTG TTGATAACTT 4173 .......... .......... .......... .......... .......... .......... 253 ATTATGCCAC TCTTTATCTG TTTCCAACAG TTTTCTGAAG CAGTACTTAT GTTTTCAGCT 4113 || .......... .......... .......... .......... .......... ........CT 255 GGATATATCT TTAATACTGT TCTTGCTCCT GCTTTTGGGC TGCCATTGAA TCCACAAGCA 4053 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGATATATCT TTAATACTGT TCTTGCTCCT GCTTTTGGGC TGCCATTGAA TCCACAAGCA 315 GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC 3993 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC 375 CAGAGAAAAG GACGGTTTTT GCTTGGGAGT GGCCAACCTT CAATTGCAGA TCTTAGCTTA 3933 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGAGAAAAG GACGGTTTTT GCTTGGGAGT GGCCAACCTT CAATTGCAGA TCTTAGCTTA 435 GTGTGTGAGA TTATGGAACT TGAGGTAAAG AGATTTTCCC TTTTCCTGCT GTTTCTTTAC 3873 |||||||||| |||||||||| |||| GTGTGTGAGA TTATGGAACT TGAG...... .......... .......... .......... 459 ATCTTTTGAT CCAGAACATA AATTACCAAG AGGAGTCTTG TTGAAATTTC TTTTATAATG 3813 .......... .......... .......... .......... .......... .......... 459 AATTTGGGTT TTGGATGTGA TGATTTTAGA TTTTGGATGA GAAGGATCGT GAGCGGATAA 3753 | |||||||||| |||||||||| |||||||||| .......... .......... .........A TTTTGGATGA GAAGGATCGT GAGCGGATAA 490 TAGGCCCATA CAAGAGAGTT TTGAAGTGGA TTGATGACAC AAAGAATGCC ATGGAACCTC 3693 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGGCCCATA CAAGAGAGTT TTGAAGTGGA TTGATGACAC AAAGAATGCC ATGGAACCTC 550 ATTTCCAAGA GGTACATGTG ATCCTCTTCA AAGCTAAAGA GAAGTTCCAC AAGCAAAGAC 3633 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTCCAAGA GGTACATGTG ATCCTCTTCA AAGCTAAAGA GAAGTTCCAC AAGCAAAGAC 610 ATGCTGTAGG AAGTAGCATT CCTCAATCGA GCAGAAAACC TGACTTGCAC 3583 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGCTGTAGG AAGTAGCATT CCTCAATCGA GCAGAAAACC TGACTTGCAC 660 hqPGS_C08HBa0251H04.1-_SGN-E210728+ (6212 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3583) ******************************************************************************** EST sequence 77 +strand 737 n (File: SGN-E263709+) 1 AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 61 TCTGCAAGTT AAATGGAATA GACTTTGAGG AGATACACAT AAATCTCTCC AAGCGCCAGC 121 AGTTATCTCC TGAATTTAAA GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG 181 GAAGATTTAA GCTTTTCGAA AGTCATGCAA TTCTTAGATA TCTGGCTTGT GCATTTCCAG 241 GAATAGCAGA TCATTGGTAC CCTGCTGACT TATACAAAAG AGCAAAGGTA GACTCTGTGT 301 TGGACTGGCA TCACTCAAAC TTGCGTCGCG GTGCAGCTGG ATATATCTTT AATACTGTTC 361 TTGCTCCTGC TTTTGGGCTG CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC 421 TTTTGGCATC TCTTGCAAAA GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC 481 TTGGGAGTGG CCAACCTTCA ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG 541 AGATTTTGGA TGAGAAGGAT CGTGAGCGGA TAATAGGCCC ATACAAGAGA GTTTTGAAGT 601 GGATTGATGA CACAAAGAAT GCCATGGAAC CTCATTTCCA AGAGGTACAT GTGATCCTCT 661 TCAAGCTNAA GAGAAGTTCA CAAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCCTCATCG 721 AGCAGAAAAC CTGACTC Predicted gene structure (within gDNA segment 9394 to 1400): Exon 1 7139 7073 ( 67 n); cDNA 1 67 ( 67 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 68 141 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 142 202 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 203 256 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 257 336 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 3909 ( 206 n); cDNA 337 542 ( 206 n); score: 1.000 Intron 6 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 7 3783 3588 ( 196 n); cDNA 543 736 ( 194 n); score: 0.969 MATCH C08HBa0251H04.1- SGN-E263709+ 0.992 738 1.001 C PGS_C08HBa0251H04.1-_SGN-E263709+ (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) Alignment (genomic DNA sequence = upper lines): AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 7080 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 60 TCTGCAAGTA AGTATTTTTC ATTTTTCTTT TCTCGAGATG TTTAAAGCTC GTAATGGAGG 7020 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 67 TTTTAATGAA TTATTTTCTA GAAATTTGAT TAACTCCGTT AGATAGGAAC AGATGATAAG 6960 .......... .......... .......... .......... .......... .......... 67 TACTGAGTAT TAGAACGGAA AGATCTATTA GATAATGAGC TATTGACACT ATTCATCAAC 6900 .......... .......... .......... .......... .......... .......... 67 AATTCGTAGT GCTATGTTAA TTCTATATGT AACTTTGAAT CTCAAAATGG AGTTTTTAGT 6840 .......... .......... .......... .......... .......... .......... 67 GACTGATCTT TCAAAAAGTT TTTTTTGTTT TCTTTGAATC CTCATATTTG TAGCTTTAGA 6780 .......... .......... .......... .......... .......... .......... 67 AGTTACGAAT ACAATAACAT CAAGAACATC CATAACGTAG TTGTTAAAAG ACTCTCCGTT 6720 .......... .......... .......... .......... .......... .......... 67 AGGCGAAATT TATTGACATA AATATGCCCT TTAACTTGAC TTTAAATTAC ATTTATGTCC 6660 .......... .......... .......... .......... .......... .......... 67 TTCAACTTTG GGTGTGGACA AGTATAAATT TGTATAAAGT TGAATAAATA GACACGTCAT 6600 .......... .......... .......... .......... .......... .......... 67 TTTTTGTCCT ACGTTATTGT GCCACGTAGG ACTCATGTGT GTACTTGTTC AAATTTATAC 6540 .......... .......... .......... .......... .......... .......... 67 AAGTTTGAGT ATCTACTTGT GCATATCCAT AATAGAAGGA CATAAATGTT AAATGAGACC 6480 .......... .......... .......... .......... .......... .......... 67 AAGTTAAATG ACGCATTTAT GTATTATGCT GCCAAGTTTA GGTGGGTAGG TTTATATGAT 6420 .......... .......... .......... .......... .......... .......... 67 TGTTTTTATA TATAGGTAAA TCAACCAAAT CAAATTTTAA AAGAATCTTT AGAGCTTATT 6360 .......... .......... .......... .......... .......... .......... 67 ACAAGGGATT TGTTAATGAG AATTTTTTAA TCAAGAAGTT AATGAAGAAG TTCTGTTGTT 6300 .......... .......... .......... .......... .......... .......... 67 GTTTCTGGAT AGTTCTGTTT CTAATTTTGA AGCTCAAAAT GGATTTTTTA ATGATTTTAA 6240 .......... .......... .......... .......... .......... .......... 67 TTTTCCTAAA GGTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 6180 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .GTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 116 CAGCAGTTAT CTCCTGAATT TAAAGGTTTG TCTTTTTTAT TTACTCCACT TTTCGCTAGA 6120 |||||||||| |||||||||| ||||| CAGCAGTTAT CTCCTGAATT TAAAG..... .......... .......... .......... 141 TATTATTTAT TTCTTAGTTT CAAGATAATT TCAAAAGTTT TCTGACTTCA TCTTCAGAAA 6060 ||| .......... .......... .......... .......... .......... .......AAA 144 TTAATCCCAT GAAGCAAGTA CCAGCTATAA TTGATGGAAG ATTTAAGCTT TTCGAAAGGT 6000 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTAATCCCAT GAAGCAAGTA CCAGCTATAA TTGATGGAAG ATTTAAGCTT TTCGAAAG.. 202 AATATTCAGC TTGAACTGAA CATTTTAGCA TTTTCCAGTA TCACAACTTT GTTAACGTCT 5940 .......... .......... .......... .......... .......... .......... 202 ATTATTCGCT GTATATGTTG CATACATCCT AAAGCTTATG ATTTAGTCTT TCATTCTCGT 5880 .......... .......... .......... .......... .......... .......... 202 AGAAGTCATG TTTGTAGCGT TCACCTAATC ACCTGTGCAT ATATACTGCA AGAAGTGAAA 5820 .......... .......... .......... .......... .......... .......... 202 AAATTTGTAA AAATTTCTAT GTTTTGTGTA CACCATGTGG GCACAAGTAA CTACTGTTGA 5760 .......... .......... .......... .......... .......... .......... 202 AAATGCCTGT TGAGACGGGA CAATTTTATC TTTTTCTGAG TTTCACATAA AGTATTTTAT 5700 .......... .......... .......... .......... .......... .......... 202 AACTTGGATA ATGTTTAAAA TTAACTAACT GATTATATGT CCTCGCAGTC ATGCAATTCT 5640 || |||||||||| .......... .......... .......... .......... ........TC ATGCAATTCT 214 TAGATATCTG GCTTGTGCAT TTCCAGGAAT AGCAGATCAT TGGTAAGTAT CGACTTATTT 5580 |||||||||| |||||||||| |||||||||| |||||||||| || TAGATATCTG GCTTGTGCAT TTCCAGGAAT AGCAGATCAT TG........ .......... 256 TGAGTATATA CTGTAGTCAA ATGCTTTGTA TCATGGAAAT AAGTACTCTT TAAGCGCTTT 5520 .......... .......... .......... .......... .......... .......... 256 TTCTAGACAT AGGATCCAAC CCGGACAGAT CAGGGATATA AACTTGATGG GTTCAACTTT 5460 .......... .......... .......... .......... .......... .......... 256 TATATTCTCA CTGCTGGTGC AATCACGTGT ATACCTAAGT TCCATGTCAA AATGTTGGGT 5400 .......... .......... .......... .......... .......... .......... 256 TAAGCAGAAC CCATATTGCA TCTGCTGCTG AACATGGCAA TCGGCATTAC GAATGGAATT 5340 .......... .......... .......... .......... .......... .......... 256 ATTCTGCAGT TGGCATCTGT GTGAATTTAA TGCCACATGT ACCTGTTATT TGATTGATAA 5280 .......... .......... .......... .......... .......... .......... 256 TTGTTTGTTG TTTTTCTAAC TTGGAGTTCA ACTAGTAGAG TTTTGATAAG AAACGCCATC 5220 .......... .......... .......... .......... .......... .......... 256 GGGTTTTGCT AAGAAACCCG TCATTTGTAC CAAAACCGAG AATAACATGA CATGTGTCTT 5160 .......... .......... .......... .......... .......... .......... 256 CTGCATACAC TTGTCACCTA ACTATTGTTA ATTAATGTGC ATTTTCATCT CATTTATTAC 5100 .......... .......... .......... .......... .......... .......... 256 TTAAGAATGG TAAAATAGTG AACGAACCAA AGGAGAACTC CCAACAGAAA AGAAGAACAA 5040 .......... .......... .......... .......... .......... .......... 256 GTAACATATT AGCAGATTGG AAAATACCAG CTCAACAAAT GAAGTATCAG CACCAAATAA 4980 .......... .......... .......... .......... .......... .......... 256 CAAACAAGCC CTATCAAAAT TTGAGAAGAG ATGACTATTG AACACAGAAG TCATCCTTTG 4920 .......... .......... .......... .......... .......... .......... 256 GAATCTCTGT ACTATACCAA CTTATCCGAA AAAAGAAAAA AAAAGTACAT CTATGGTGCC 4860 .......... .......... .......... .......... .......... .......... 256 TATGAACAAT GCTGCTCTTC ATTCTTAGTG ATAGACTAAT GTACACACTC TTAGGTTGTA 4800 .......... .......... .......... .......... .......... .......... 256 TAAATTCTTA TCCTGGTTAG TGTTTGATTT AAATCCTCTT TCTTACACTT TCCTATTGAA 4740 .......... .......... .......... .......... .......... .......... 256 GGTACCCTGC TGACTTATAC AAAAGAGCAA AGGTAGACTC TGTGTTGGAC TGGCATCACT 4680 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .GTACCCTGC TGACTTATAC AAAAGAGCAA AGGTAGACTC TGTGTTGGAC TGGCATCACT 315 CAAACTTGCG TCGCGGTGCA GGTGCTTATA TCAGATATTG AGATCATTTC AGTTTCCGGT 4620 |||||||||| |||||||||| | CAAACTTGCG TCGCGGTGCA G......... .......... .......... .......... 336 TGAATTATGA TTACTTTACG TTGACCTTAC TTGAGTTAAT TTAGTGGTGA ATGCTGGGAT 4560 .......... .......... .......... .......... .......... .......... 336 ATGCAAGAAC TTGGGCAAAA AGATGCATAC AGGCTACAGT TAAGGCAATG ACTGGATTAA 4500 .......... .......... .......... .......... .......... .......... 336 AATTTTATCC CAAATAAGAT TAATCAAGGA AATCTGTTTG TATGATCAGT TTTAAAAAAG 4440 .......... .......... .......... .......... .......... .......... 336 AGTATCTGCT TGTCATGATG CTCTTTGCTA AGATTCTCGC TACTAACCTG ATACAAGAGA 4380 .......... .......... .......... .......... .......... .......... 336 GTTGCTGCAA GCCTACAAGT TTCTTAGTCT ATCTTTAGGA CTTGATTCCT CTAATTGAAA 4320 .......... .......... .......... .......... .......... .......... 336 CACCTGACCT TTCTCAAACC TCTAATTGTG AGTCTAGCTA CTGCATGATG GTGACTTCTT 4260 .......... .......... .......... .......... .......... .......... 336 TTCTTCAACA ATAACAGTGA CTAAAACCAC TAGGATAGGT AGTCTTTTCA CCAATGTTCT 4200 .......... .......... .......... .......... .......... .......... 336 ATAATTACTA CTTCTTGTTG ATAACTTATT ATGCCACTCT TTATCTGTTT CCAACAGTTT 4140 .......... .......... .......... .......... .......... .......... 336 TCTGAAGCAG TACTTATGTT TTCAGCTGGA TATATCTTTA ATACTGTTCT TGCTCCTGCT 4080 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....CTGGA TATATCTTTA ATACTGTTCT TGCTCCTGCT 371 TTTGGGCTGC CATTGAATCC ACAAGCAGCA GCAGAAGCTG AGAAAGTCCT TTTGGCATCT 4020 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGGGCTGC CATTGAATCC ACAAGCAGCA GCAGAAGCTG AGAAAGTCCT TTTGGCATCT 431 CTTGCAAAAG TCGAGTCTGT TTGGCTCCAG AGAAAAGGAC GGTTTTTGCT TGGGAGTGGC 3960 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGCAAAAG TCGAGTCTGT TTGGCTCCAG AGAAAAGGAC GGTTTTTGCT TGGGAGTGGC 491 CAACCTTCAA TTGCAGATCT TAGCTTAGTG TGTGAGATTA TGGAACTTGA GGTAAAGAGA 3900 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CAACCTTCAA TTGCAGATCT TAGCTTAGTG TGTGAGATTA TGGAACTTGA G......... 542 TTTTCCCTTT TCCTGCTGTT TCTTTACATC TTTTGATCCA GAACATAAAT TACCAAGAGG 3840 .......... .......... .......... .......... .......... .......... 542 AGTCTTGTTG AAATTTCTTT TATAATGAAT TTGGGTTTTG GATGTGATGA TTTTAGATTT 3780 |||| .......... .......... .......... .......... .......... ......ATTT 546 TGGATGAGAA GGATCGTGAG CGGATAATAG GCCCATACAA GAGAGTTTTG AAGTGGATTG 3720 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGATGAGAA GGATCGTGAG CGGATAATAG GCCCATACAA GAGAGTTTTG AAGTGGATTG 606 ATGACACAAA GAATGCCATG GAACCTCATT TCCAAGAGGT ACATGTGATC CTCTTCAAAG 3660 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| ||| ATGACACAAA GAATGCCATG GAACCTCATT TCCAAGAGGT ACATGTGATC CTCTTC-AAG 665 CTAAAGAGAA GTTCCACAAG CAAAGACATG CTGTAGGAAG TAGCATTCCT CAATCGAGCA 3600 || ||||||| ||| |||||| |||||||||| |||||||||| ||||||||| |||||||| CTNAAGAGAA GTT-CACAAG CAAAGACATG CTGTAGGAAG TAGCATTCCC TCATCGAGCA 724 GAAAACCTGA CT 3588 |||||||||| || GAAAACCTGA CT 736 hqPGS_C08HBa0251H04.1-_SGN-E263709+ (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) ******************************************************************************** EST sequence 81 +strand 736 n (File: SGN-E261719+) 1 ATGACGTTGA AATTATACGT AGATCGTATG TCCCAACCTT CTCGTGCAGT TATCATCTTC 61 TGCAAGTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA GCGCCAGCAG 121 TTATCTCCTG AATTTAAAGA AATTAATCCC ATGAAGCAAG TACCAGCTAT AATTGATGGA 181 AGATTTAAGC TTTTCGAAAG TCATGCAATT CTTAGATATC TGGCTTGTGC ATTTCCAGGA 241 ATAGCAGATC ATTGGTACCC TGCTGACTTA TACAAAAGAG CAAAGGTAGA CTCTGTGTTG 301 GACTGGCATC ACTCAAACTT GCGTCGCGGT GCAGCTGGAT ATATCTTTAA TACTGTTCTT 361 GCTCCTGCTT TTGGGCTGCC ATTGAATCCA CAAGCAGCAG CAGAAGCTGA GAAAGTCCTT 421 TTGGCATCTC TTGCAAAAGT CGAGTCTGTT TGGCTCCAGA GAAAAGGACG GTTTTTGCTT 481 GGGAGTGGCC AACCTTCAAT TGCAGATCTT AGCTTAGTGT GTGAGATTAT GGAACTTGAG 541 ATTTTGGATG AGAAGGATCG TGAGCGGATA ATAGGCCCAT ACAAGAGAGT TTTGAAGTGG 601 ATTGATGACA CAAAGAATGC CATGGAACCT CATTTCCAAG AGGTACATGT GATCCTCTTC 661 AAAGCTAAAG AGAAAGTTCA CAAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCCTCATCG 721 AGCAGAAAAC CTGACT Predicted gene structure (within gDNA segment 9374 to 1453): Exon 1 7137 7073 ( 65 n); cDNA 1 65 ( 65 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 66 139 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 140 200 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 201 254 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 255 334 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 3909 ( 206 n); cDNA 335 540 ( 206 n); score: 1.000 Intron 6 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 7 3783 3588 ( 196 n); cDNA 541 736 ( 196 n); score: 0.969 MATCH C08HBa0251H04.1- SGN-E261719+ 0.992 736 1.000 C PGS_C08HBa0251H04.1-_SGN-E261719+ (7137 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) Alignment (genomic DNA sequence = upper lines): ATGACGTTGA AATTATACGT AGATCGTATG TCCCAACCTT CTCGTGCAGT TATCATCTTC 7078 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGACGTTGA AATTATACGT AGATCGTATG TCCCAACCTT CTCGTGCAGT TATCATCTTC 60 TGCAAGTAAG TATTTTTCAT TTTTCTTTTC TCGAGATGTT TAAAGCTCGT AATGGAGGTT 7018 ||||| TGCAA..... .......... .......... .......... .......... .......... 65 TTAATGAATT ATTTTCTAGA AATTTGATTA ACTCCGTTAG ATAGGAACAG ATGATAAGTA 6958 .......... .......... .......... .......... .......... .......... 65 CTGAGTATTA GAACGGAAAG ATCTATTAGA TAATGAGCTA TTGACACTAT TCATCAACAA 6898 .......... .......... .......... .......... .......... .......... 65 TTCGTAGTGC TATGTTAATT CTATATGTAA CTTTGAATCT CAAAATGGAG TTTTTAGTGA 6838 .......... .......... .......... .......... .......... .......... 65 CTGATCTTTC AAAAAGTTTT TTTTGTTTTC TTTGAATCCT CATATTTGTA GCTTTAGAAG 6778 .......... .......... .......... .......... .......... .......... 65 TTACGAATAC AATAACATCA AGAACATCCA TAACGTAGTT GTTAAAAGAC TCTCCGTTAG 6718 .......... .......... .......... .......... .......... .......... 65 GCGAAATTTA TTGACATAAA TATGCCCTTT AACTTGACTT TAAATTACAT TTATGTCCTT 6658 .......... .......... .......... .......... .......... .......... 65 CAACTTTGGG TGTGGACAAG TATAAATTTG TATAAAGTTG AATAAATAGA CACGTCATTT 6598 .......... .......... .......... .......... .......... .......... 65 TTTGTCCTAC GTTATTGTGC CACGTAGGAC TCATGTGTGT ACTTGTTCAA ATTTATACAA 6538 .......... .......... .......... .......... .......... .......... 65 GTTTGAGTAT CTACTTGTGC ATATCCATAA TAGAAGGACA TAAATGTTAA ATGAGACCAA 6478 .......... .......... .......... .......... .......... .......... 65 GTTAAATGAC GCATTTATGT ATTATGCTGC CAAGTTTAGG TGGGTAGGTT TATATGATTG 6418 .......... .......... .......... .......... .......... .......... 65 TTTTTATATA TAGGTAAATC AACCAAATCA AATTTTAAAA GAATCTTTAG AGCTTATTAC 6358 .......... .......... .......... .......... .......... .......... 65 AAGGGATTTG TTAATGAGAA TTTTTTAATC AAGAAGTTAA TGAAGAAGTT CTGTTGTTGT 6298 .......... .......... .......... .......... .......... .......... 65 TTCTGGATAG TTCTGTTTCT AATTTTGAAG CTCAAAATGG ATTTTTTAAT GATTTTAATT 6238 .......... .......... .......... .......... .......... .......... 65 TTCCTAAAGG TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA 6178 | |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .........G TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA 116 GCAGTTATCT CCTGAATTTA AAGGTTTGTC TTTTTTATTT ACTCCACTTT TCGCTAGATA 6118 |||||||||| |||||||||| ||| GCAGTTATCT CCTGAATTTA AAG....... .......... .......... .......... 139 TTATTTATTT CTTAGTTTCA AGATAATTTC AAAAGTTTTC TGACTTCATC TTCAGAAATT 6058 ||||| .......... .......... .......... .......... .......... .....AAATT 144 AATCCCATGA AGCAAGTACC AGCTATAATT GATGGAAGAT TTAAGCTTTT CGAAAGGTAA 5998 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| AATCCCATGA AGCAAGTACC AGCTATAATT GATGGAAGAT TTAAGCTTTT CGAAAG.... 200 TATTCAGCTT GAACTGAACA TTTTAGCATT TTCCAGTATC ACAACTTTGT TAACGTCTAT 5938 .......... .......... .......... .......... .......... .......... 200 TATTCGCTGT ATATGTTGCA TACATCCTAA AGCTTATGAT TTAGTCTTTC ATTCTCGTAG 5878 .......... .......... .......... .......... .......... .......... 200 AAGTCATGTT TGTAGCGTTC ACCTAATCAC CTGTGCATAT ATACTGCAAG AAGTGAAAAA 5818 .......... .......... .......... .......... .......... .......... 200 ATTTGTAAAA ATTTCTATGT TTTGTGTACA CCATGTGGGC ACAAGTAACT ACTGTTGAAA 5758 .......... .......... .......... .......... .......... .......... 200 ATGCCTGTTG AGACGGGACA ATTTTATCTT TTTCTGAGTT TCACATAAAG TATTTTATAA 5698 .......... .......... .......... .......... .......... .......... 200 CTTGGATAAT GTTTAAAATT AACTAACTGA TTATATGTCC TCGCAGTCAT GCAATTCTTA 5638 |||| |||||||||| .......... .......... .......... .......... ......TCAT GCAATTCTTA 214 GATATCTGGC TTGTGCATTT CCAGGAATAG CAGATCATTG GTAAGTATCG ACTTATTTTG 5578 |||||||||| |||||||||| |||||||||| |||||||||| GATATCTGGC TTGTGCATTT CCAGGAATAG CAGATCATTG .......... .......... 254 AGTATATACT GTAGTCAAAT GCTTTGTATC ATGGAAATAA GTACTCTTTA AGCGCTTTTT 5518 .......... .......... .......... .......... .......... .......... 254 CTAGACATAG GATCCAACCC GGACAGATCA GGGATATAAA CTTGATGGGT TCAACTTTTA 5458 .......... .......... .......... .......... .......... .......... 254 TATTCTCACT GCTGGTGCAA TCACGTGTAT ACCTAAGTTC CATGTCAAAA TGTTGGGTTA 5398 .......... .......... .......... .......... .......... .......... 254 AGCAGAACCC ATATTGCATC TGCTGCTGAA CATGGCAATC GGCATTACGA ATGGAATTAT 5338 .......... .......... .......... .......... .......... .......... 254 TCTGCAGTTG GCATCTGTGT GAATTTAATG CCACATGTAC CTGTTATTTG ATTGATAATT 5278 .......... .......... .......... .......... .......... .......... 254 GTTTGTTGTT TTTCTAACTT GGAGTTCAAC TAGTAGAGTT TTGATAAGAA ACGCCATCGG 5218 .......... .......... .......... .......... .......... .......... 254 GTTTTGCTAA GAAACCCGTC ATTTGTACCA AAACCGAGAA TAACATGACA TGTGTCTTCT 5158 .......... .......... .......... .......... .......... .......... 254 GCATACACTT GTCACCTAAC TATTGTTAAT TAATGTGCAT TTTCATCTCA TTTATTACTT 5098 .......... .......... .......... .......... .......... .......... 254 AAGAATGGTA AAATAGTGAA CGAACCAAAG GAGAACTCCC AACAGAAAAG AAGAACAAGT 5038 .......... .......... .......... .......... .......... .......... 254 AACATATTAG CAGATTGGAA AATACCAGCT CAACAAATGA AGTATCAGCA CCAAATAACA 4978 .......... .......... .......... .......... .......... .......... 254 AACAAGCCCT ATCAAAATTT GAGAAGAGAT GACTATTGAA CACAGAAGTC ATCCTTTGGA 4918 .......... .......... .......... .......... .......... .......... 254 ATCTCTGTAC TATACCAACT TATCCGAAAA AAGAAAAAAA AAGTACATCT ATGGTGCCTA 4858 .......... .......... .......... .......... .......... .......... 254 TGAACAATGC TGCTCTTCAT TCTTAGTGAT AGACTAATGT ACACACTCTT AGGTTGTATA 4798 .......... .......... .......... .......... .......... .......... 254 AATTCTTATC CTGGTTAGTG TTTGATTTAA ATCCTCTTTC TTACACTTTC CTATTGAAGG 4738 | .......... .......... .......... .......... .......... .........G 255 TACCCTGCTG ACTTATACAA AAGAGCAAAG GTAGACTCTG TGTTGGACTG GCATCACTCA 4678 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACCCTGCTG ACTTATACAA AAGAGCAAAG GTAGACTCTG TGTTGGACTG GCATCACTCA 315 AACTTGCGTC GCGGTGCAGG TGCTTATATC AGATATTGAG ATCATTTCAG TTTCCGGTTG 4618 |||||||||| ||||||||| AACTTGCGTC GCGGTGCAG. .......... .......... .......... .......... 334 AATTATGATT ACTTTACGTT GACCTTACTT GAGTTAATTT AGTGGTGAAT GCTGGGATAT 4558 .......... .......... .......... .......... .......... .......... 334 GCAAGAACTT GGGCAAAAAG ATGCATACAG GCTACAGTTA AGGCAATGAC TGGATTAAAA 4498 .......... .......... .......... .......... .......... .......... 334 TTTTATCCCA AATAAGATTA ATCAAGGAAA TCTGTTTGTA TGATCAGTTT TAAAAAAGAG 4438 .......... .......... .......... .......... .......... .......... 334 TATCTGCTTG TCATGATGCT CTTTGCTAAG ATTCTCGCTA CTAACCTGAT ACAAGAGAGT 4378 .......... .......... .......... .......... .......... .......... 334 TGCTGCAAGC CTACAAGTTT CTTAGTCTAT CTTTAGGACT TGATTCCTCT AATTGAAACA 4318 .......... .......... .......... .......... .......... .......... 334 CCTGACCTTT CTCAAACCTC TAATTGTGAG TCTAGCTACT GCATGATGGT GACTTCTTTT 4258 .......... .......... .......... .......... .......... .......... 334 CTTCAACAAT AACAGTGACT AAAACCACTA GGATAGGTAG TCTTTTCACC AATGTTCTAT 4198 .......... .......... .......... .......... .......... .......... 334 AATTACTACT TCTTGTTGAT AACTTATTAT GCCACTCTTT ATCTGTTTCC AACAGTTTTC 4138 .......... .......... .......... .......... .......... .......... 334 TGAAGCAGTA CTTATGTTTT CAGCTGGATA TATCTTTAAT ACTGTTCTTG CTCCTGCTTT 4078 ||||||| |||||||||| |||||||||| |||||||||| .......... .......... ...CTGGATA TATCTTTAAT ACTGTTCTTG CTCCTGCTTT 371 TGGGCTGCCA TTGAATCCAC AAGCAGCAGC AGAAGCTGAG AAAGTCCTTT TGGCATCTCT 4018 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGGCTGCCA TTGAATCCAC AAGCAGCAGC AGAAGCTGAG AAAGTCCTTT TGGCATCTCT 431 TGCAAAAGTC GAGTCTGTTT GGCTCCAGAG AAAAGGACGG TTTTTGCTTG GGAGTGGCCA 3958 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCAAAAGTC GAGTCTGTTT GGCTCCAGAG AAAAGGACGG TTTTTGCTTG GGAGTGGCCA 491 ACCTTCAATT GCAGATCTTA GCTTAGTGTG TGAGATTATG GAACTTGAGG TAAAGAGATT 3898 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ACCTTCAATT GCAGATCTTA GCTTAGTGTG TGAGATTATG GAACTTGAG. .......... 540 TTCCCTTTTC CTGCTGTTTC TTTACATCTT TTGATCCAGA ACATAAATTA CCAAGAGGAG 3838 .......... .......... .......... .......... .......... .......... 540 TCTTGTTGAA ATTTCTTTTA TAATGAATTT GGGTTTTGGA TGTGATGATT TTAGATTTTG 3778 |||||| .......... .......... .......... .......... .......... ....ATTTTG 546 GATGAGAAGG ATCGTGAGCG GATAATAGGC CCATACAAGA GAGTTTTGAA GTGGATTGAT 3718 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATGAGAAGG ATCGTGAGCG GATAATAGGC CCATACAAGA GAGTTTTGAA GTGGATTGAT 606 GACACAAAGA ATGCCATGGA ACCTCATTTC CAAGAGGTAC ATGTGATCCT CTTCAAAGCT 3658 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACACAAAGA ATGCCATGGA ACCTCATTTC CAAGAGGTAC ATGTGATCCT CTTCAAAGCT 666 AAAGAGAAGT TCCACAAGCA AAGACATGCT GTAGGAAGTA GCATTCCTCA ATCGAGCAGA 3598 |||||||| | |||||||| |||||||||| |||||||||| ||||||| |||||||||| AAAGAGAAAG TTCACAAGCA AAGACATGCT GTAGGAAGTA GCATTCCCTC ATCGAGCAGA 726 AAACCTGACT 3588 |||||||||| AAACCTGACT 736 hqPGS_C08HBa0251H04.1-_SGN-E261719+ (7137 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) ******************************************************************************** EST sequence 136 +strand 737 n (File: SGN-E260745+) 1 AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 61 TCTGCAAGTT AAATGGAATA GACTTTGAGG AGATACACAT AAATCTCTCC AAGCGCCAGC 121 AGTTATCTCC TGAATTTAAA GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG 181 GAAGATTTAA GCTTTTCGAA AGTCATGCAA TTCTTAGATA TCTGGCTTGT GCATTTCCAG 241 GAATAGCAGA TCATTGGTAC CCTGCTGACT TATACAAAAG AGCAAAGGTA GACTCTGTGT 301 TGGACTGGCA TCACTCAAAC TTGCGTCGCG GTGCAGCTGG ATATATCTTT AATACTGTTC 361 TTGCTCCTGC TTTTGGGCTG CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC 421 TTTTGGCATC TCTTGCAAAA GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC 481 TTGGGAGTGG CCAACCTTCA ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG 541 AGATTTTGGA TGAGAAGGAT CGTGAGCGGA TAATAGGCCC ATACAAGAAA GTTTTGAAGT 601 GGATTGATGA CACAAAGAAT GCCATGGAAC CTCATTTTCA AGAGGTACAT GTGATCCTCT 661 TCAAAGCTAA AGAGAAAGTC CACNAGCAAA GACATGCTGT AGGAAGTAGC ATTCCTCAAT 721 CGAGCAGAAA ACCTGAC Predicted gene structure (within gDNA segment 8863 to 1463): Exon 1 7139 7073 ( 67 n); cDNA 1 67 ( 67 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 68 141 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 142 202 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 203 256 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 257 336 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 3909 ( 206 n); cDNA 337 542 ( 206 n); score: 1.000 Intron 6 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 0.98) Exon 7 3783 3589 ( 195 n); cDNA 543 737 ( 195 n); score: 0.974 MATCH C08HBa0251H04.1- SGN-E260745+ 0.993 737 1.000 C PGS_C08HBa0251H04.1-_SGN-E260745+ (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3589) Alignment (genomic DNA sequence = upper lines): AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 7080 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 60 TCTGCAAGTA AGTATTTTTC ATTTTTCTTT TCTCGAGATG TTTAAAGCTC GTAATGGAGG 7020 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 67 TTTTAATGAA TTATTTTCTA GAAATTTGAT TAACTCCGTT AGATAGGAAC AGATGATAAG 6960 .......... .......... .......... .......... .......... .......... 67 TACTGAGTAT TAGAACGGAA AGATCTATTA GATAATGAGC TATTGACACT ATTCATCAAC 6900 .......... .......... .......... .......... .......... .......... 67 AATTCGTAGT GCTATGTTAA TTCTATATGT AACTTTGAAT CTCAAAATGG AGTTTTTAGT 6840 .......... .......... .......... .......... .......... .......... 67 GACTGATCTT TCAAAAAGTT TTTTTTGTTT TCTTTGAATC CTCATATTTG TAGCTTTAGA 6780 .......... .......... .......... .......... .......... .......... 67 AGTTACGAAT ACAATAACAT CAAGAACATC CATAACGTAG TTGTTAAAAG ACTCTCCGTT 6720 .......... .......... .......... .......... .......... .......... 67 AGGCGAAATT TATTGACATA AATATGCCCT TTAACTTGAC TTTAAATTAC ATTTATGTCC 6660 .......... .......... .......... .......... .......... .......... 67 TTCAACTTTG GGTGTGGACA AGTATAAATT TGTATAAAGT TGAATAAATA GACACGTCAT 6600 .......... .......... .......... .......... .......... .......... 67 TTTTTGTCCT ACGTTATTGT GCCACGTAGG ACTCATGTGT GTACTTGTTC AAATTTATAC 6540 .......... .......... .......... .......... .......... .......... 67 AAGTTTGAGT ATCTACTTGT GCATATCCAT AATAGAAGGA CATAAATGTT AAATGAGACC 6480 .......... .......... .......... .......... .......... .......... 67 AAGTTAAATG ACGCATTTAT GTATTATGCT GCCAAGTTTA GGTGGGTAGG TTTATATGAT 6420 .......... .......... .......... .......... .......... .......... 67 TGTTTTTATA TATAGGTAAA TCAACCAAAT CAAATTTTAA AAGAATCTTT AGAGCTTATT 6360 .......... .......... .......... .......... .......... .......... 67 ACAAGGGATT TGTTAATGAG AATTTTTTAA TCAAGAAGTT AATGAAGAAG TTCTGTTGTT 6300 .......... .......... .......... .......... .......... .......... 67 GTTTCTGGAT AGTTCTGTTT CTAATTTTGA AGCTCAAAAT GGATTTTTTA ATGATTTTAA 6240 .......... .......... .......... .......... .......... .......... 67 TTTTCCTAAA GGTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 6180 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .GTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 116 CAGCAGTTAT CTCCTGAATT TAAAGGTTTG TCTTTTTTAT TTACTCCACT TTTCGCTAGA 6120 |||||||||| |||||||||| ||||| CAGCAGTTAT CTCCTGAATT TAAAG..... .......... .......... .......... 141 TATTATTTAT TTCTTAGTTT CAAGATAATT TCAAAAGTTT TCTGACTTCA TCTTCAGAAA 6060 ||| .......... .......... .......... .......... .......... .......AAA 144 TTAATCCCAT GAAGCAAGTA CCAGCTATAA TTGATGGAAG ATTTAAGCTT TTCGAAAGGT 6000 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTAATCCCAT GAAGCAAGTA CCAGCTATAA TTGATGGAAG ATTTAAGCTT TTCGAAAG.. 202 AATATTCAGC TTGAACTGAA CATTTTAGCA TTTTCCAGTA TCACAACTTT GTTAACGTCT 5940 .......... .......... .......... .......... .......... .......... 202 ATTATTCGCT GTATATGTTG CATACATCCT AAAGCTTATG ATTTAGTCTT TCATTCTCGT 5880 .......... .......... .......... .......... .......... .......... 202 AGAAGTCATG TTTGTAGCGT TCACCTAATC ACCTGTGCAT ATATACTGCA AGAAGTGAAA 5820 .......... .......... .......... .......... .......... .......... 202 AAATTTGTAA AAATTTCTAT GTTTTGTGTA CACCATGTGG GCACAAGTAA CTACTGTTGA 5760 .......... .......... .......... .......... .......... .......... 202 AAATGCCTGT TGAGACGGGA CAATTTTATC TTTTTCTGAG TTTCACATAA AGTATTTTAT 5700 .......... .......... .......... .......... .......... .......... 202 AACTTGGATA ATGTTTAAAA TTAACTAACT GATTATATGT CCTCGCAGTC ATGCAATTCT 5640 || |||||||||| .......... .......... .......... .......... ........TC ATGCAATTCT 214 TAGATATCTG GCTTGTGCAT TTCCAGGAAT AGCAGATCAT TGGTAAGTAT CGACTTATTT 5580 |||||||||| |||||||||| |||||||||| |||||||||| || TAGATATCTG GCTTGTGCAT TTCCAGGAAT AGCAGATCAT TG........ .......... 256 TGAGTATATA CTGTAGTCAA ATGCTTTGTA TCATGGAAAT AAGTACTCTT TAAGCGCTTT 5520 .......... .......... .......... .......... .......... .......... 256 TTCTAGACAT AGGATCCAAC CCGGACAGAT CAGGGATATA AACTTGATGG GTTCAACTTT 5460 .......... .......... .......... .......... .......... .......... 256 TATATTCTCA CTGCTGGTGC AATCACGTGT ATACCTAAGT TCCATGTCAA AATGTTGGGT 5400 .......... .......... .......... .......... .......... .......... 256 TAAGCAGAAC CCATATTGCA TCTGCTGCTG AACATGGCAA TCGGCATTAC GAATGGAATT 5340 .......... .......... .......... .......... .......... .......... 256 ATTCTGCAGT TGGCATCTGT GTGAATTTAA TGCCACATGT ACCTGTTATT TGATTGATAA 5280 .......... .......... .......... .......... .......... .......... 256 TTGTTTGTTG TTTTTCTAAC TTGGAGTTCA ACTAGTAGAG TTTTGATAAG AAACGCCATC 5220 .......... .......... .......... .......... .......... .......... 256 GGGTTTTGCT AAGAAACCCG TCATTTGTAC CAAAACCGAG AATAACATGA CATGTGTCTT 5160 .......... .......... .......... .......... .......... .......... 256 CTGCATACAC TTGTCACCTA ACTATTGTTA ATTAATGTGC ATTTTCATCT CATTTATTAC 5100 .......... .......... .......... .......... .......... .......... 256 TTAAGAATGG TAAAATAGTG AACGAACCAA AGGAGAACTC CCAACAGAAA AGAAGAACAA 5040 .......... .......... .......... .......... .......... .......... 256 GTAACATATT AGCAGATTGG AAAATACCAG CTCAACAAAT GAAGTATCAG CACCAAATAA 4980 .......... .......... .......... .......... .......... .......... 256 CAAACAAGCC CTATCAAAAT TTGAGAAGAG ATGACTATTG AACACAGAAG TCATCCTTTG 4920 .......... .......... .......... .......... .......... .......... 256 GAATCTCTGT ACTATACCAA CTTATCCGAA AAAAGAAAAA AAAAGTACAT CTATGGTGCC 4860 .......... .......... .......... .......... .......... .......... 256 TATGAACAAT GCTGCTCTTC ATTCTTAGTG ATAGACTAAT GTACACACTC TTAGGTTGTA 4800 .......... .......... .......... .......... .......... .......... 256 TAAATTCTTA TCCTGGTTAG TGTTTGATTT AAATCCTCTT TCTTACACTT TCCTATTGAA 4740 .......... .......... .......... .......... .......... .......... 256 GGTACCCTGC TGACTTATAC AAAAGAGCAA AGGTAGACTC TGTGTTGGAC TGGCATCACT 4680 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .GTACCCTGC TGACTTATAC AAAAGAGCAA AGGTAGACTC TGTGTTGGAC TGGCATCACT 315 CAAACTTGCG TCGCGGTGCA GGTGCTTATA TCAGATATTG AGATCATTTC AGTTTCCGGT 4620 |||||||||| |||||||||| | CAAACTTGCG TCGCGGTGCA G......... .......... .......... .......... 336 TGAATTATGA TTACTTTACG TTGACCTTAC TTGAGTTAAT TTAGTGGTGA ATGCTGGGAT 4560 .......... .......... .......... .......... .......... .......... 336 ATGCAAGAAC TTGGGCAAAA AGATGCATAC AGGCTACAGT TAAGGCAATG ACTGGATTAA 4500 .......... .......... .......... .......... .......... .......... 336 AATTTTATCC CAAATAAGAT TAATCAAGGA AATCTGTTTG TATGATCAGT TTTAAAAAAG 4440 .......... .......... .......... .......... .......... .......... 336 AGTATCTGCT TGTCATGATG CTCTTTGCTA AGATTCTCGC TACTAACCTG ATACAAGAGA 4380 .......... .......... .......... .......... .......... .......... 336 GTTGCTGCAA GCCTACAAGT TTCTTAGTCT ATCTTTAGGA CTTGATTCCT CTAATTGAAA 4320 .......... .......... .......... .......... .......... .......... 336 CACCTGACCT TTCTCAAACC TCTAATTGTG AGTCTAGCTA CTGCATGATG GTGACTTCTT 4260 .......... .......... .......... .......... .......... .......... 336 TTCTTCAACA ATAACAGTGA CTAAAACCAC TAGGATAGGT AGTCTTTTCA CCAATGTTCT 4200 .......... .......... .......... .......... .......... .......... 336 ATAATTACTA CTTCTTGTTG ATAACTTATT ATGCCACTCT TTATCTGTTT CCAACAGTTT 4140 .......... .......... .......... .......... .......... .......... 336 TCTGAAGCAG TACTTATGTT TTCAGCTGGA TATATCTTTA ATACTGTTCT TGCTCCTGCT 4080 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....CTGGA TATATCTTTA ATACTGTTCT TGCTCCTGCT 371 TTTGGGCTGC CATTGAATCC ACAAGCAGCA GCAGAAGCTG AGAAAGTCCT TTTGGCATCT 4020 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGGGCTGC CATTGAATCC ACAAGCAGCA GCAGAAGCTG AGAAAGTCCT TTTGGCATCT 431 CTTGCAAAAG TCGAGTCTGT TTGGCTCCAG AGAAAAGGAC GGTTTTTGCT TGGGAGTGGC 3960 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGCAAAAG TCGAGTCTGT TTGGCTCCAG AGAAAAGGAC GGTTTTTGCT TGGGAGTGGC 491 CAACCTTCAA TTGCAGATCT TAGCTTAGTG TGTGAGATTA TGGAACTTGA GGTAAAGAGA 3900 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CAACCTTCAA TTGCAGATCT TAGCTTAGTG TGTGAGATTA TGGAACTTGA G......... 542 TTTTCCCTTT TCCTGCTGTT TCTTTACATC TTTTGATCCA GAACATAAAT TACCAAGAGG 3840 .......... .......... .......... .......... .......... .......... 542 AGTCTTGTTG AAATTTCTTT TATAATGAAT TTGGGTTTTG GATGTGATGA TTTTAGATTT 3780 |||| .......... .......... .......... .......... .......... ......ATTT 546 TGGATGAGAA GGATCGTGAG CGGATAATAG GCCCATACAA GAGAGTTTTG AAGTGGATTG 3720 |||||||||| |||||||||| |||||||||| |||||||||| || ||||||| |||||||||| TGGATGAGAA GGATCGTGAG CGGATAATAG GCCCATACAA GAAAGTTTTG AAGTGGATTG 606 ATGACACAAA GAATGCCATG GAACCTCATT TCCAAGAGGT ACATGTGATC CTCTTCAAAG 3660 |||||||||| |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| ATGACACAAA GAATGCCATG GAACCTCATT TTCAAGAGGT ACATGTGATC CTCTTCAAAG 666 CTAAAGAGAA GTTCCACAAG CAAAGACATG CTGTAGGAAG TAGCATTCCT CAATCGAGCA 3600 |||||||||| ||||| || |||||||||| |||||||||| |||||||||| |||||||||| CTAAAGAGAA AGTCCACNAG CAAAGACATG CTGTAGGAAG TAGCATTCCT CAATCGAGCA 726 GAAAACCTGA C 3589 |||||||||| | GAAAACCTGA C 737 hqPGS_C08HBa0251H04.1-_SGN-E260745+ (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3589) ******************************************************************************** EST sequence 86 +strand 688 n (File: SGN-E373791+) 1 GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 61 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 121 TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA GCAGTTATCT 181 CCTGAATTTA AAGAAATTAA TCCCATGAAG CAAGTACCAG CTATAATTGA TGGAAGATTT 241 AAGCTTTTCG AAAGTCATGC AATTCTTAGA TATCTGGCTT GTGCATTTCC AGGAATAGCA 301 GATCATTGGT ACCCTGCTGA CTTATACAAA AGAGCAAAGG TAGACTCTGT GTTGGACTGG 361 CATCACTCAA ACTTGCGTCG CGGTGCAGCT GGATATATCT TTAATACTGT TCTTGCTCCT 421 GCTTTTGGGC TGCCATTGAA TCCACAAGCA GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA 481 TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC CAGAGAAAAG GACGGTTTTT GCTTGGGAGT 541 GGCCAACCTT CAATTGCAGA TCTTAGCTTA GTGTGTGAGA TTATGGAACT TGAGATTTTG 601 GATGAGAAGG ATCGTGAGCG GATAATAGGC CCATACAAGA GAGTTTTGAA GTGGATTGAT 661 GACACANAGA ATGCCATGGA ACCTCATT Predicted gene structure (within gDNA segment 9383 to 1789): Exon 1 7191 7073 ( 119 n); cDNA 1 119 ( 119 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 120 193 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 194 254 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 255 308 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 309 388 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 3909 ( 206 n); cDNA 389 594 ( 206 n); score: 1.000 Intron 6 3908 3784 ( 125 n); Pd: 0.996 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 7 3783 3690 ( 94 n); cDNA 595 688 ( 94 n); score: 0.989 MATCH C08HBa0251H04.1- SGN-E373791+ 0.999 688 1.000 C PGS_C08HBa0251H04.1-_SGN-E373791+ (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3690) Alignment (genomic DNA sequence = upper lines): GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 7132 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 60 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 7072 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAA. 119 TAAGTATTTT TCATTTTTCT TTTCTCGAGA TGTTTAAAGC TCGTAATGGA GGTTTTAATG 7012 .......... .......... .......... .......... .......... .......... 119 AATTATTTTC TAGAAATTTG ATTAACTCCG TTAGATAGGA ACAGATGATA AGTACTGAGT 6952 .......... .......... .......... .......... .......... .......... 119 ATTAGAACGG AAAGATCTAT TAGATAATGA GCTATTGACA CTATTCATCA ACAATTCGTA 6892 .......... .......... .......... .......... .......... .......... 119 GTGCTATGTT AATTCTATAT GTAACTTTGA ATCTCAAAAT GGAGTTTTTA GTGACTGATC 6832 .......... .......... .......... .......... .......... .......... 119 TTTCAAAAAG TTTTTTTTGT TTTCTTTGAA TCCTCATATT TGTAGCTTTA GAAGTTACGA 6772 .......... .......... .......... .......... .......... .......... 119 ATACAATAAC ATCAAGAACA TCCATAACGT AGTTGTTAAA AGACTCTCCG TTAGGCGAAA 6712 .......... .......... .......... .......... .......... .......... 119 TTTATTGACA TAAATATGCC CTTTAACTTG ACTTTAAATT ACATTTATGT CCTTCAACTT 6652 .......... .......... .......... .......... .......... .......... 119 TGGGTGTGGA CAAGTATAAA TTTGTATAAA GTTGAATAAA TAGACACGTC ATTTTTTGTC 6592 .......... .......... .......... .......... .......... .......... 119 CTACGTTATT GTGCCACGTA GGACTCATGT GTGTACTTGT TCAAATTTAT ACAAGTTTGA 6532 .......... .......... .......... .......... .......... .......... 119 GTATCTACTT GTGCATATCC ATAATAGAAG GACATAAATG TTAAATGAGA CCAAGTTAAA 6472 .......... .......... .......... .......... .......... .......... 119 TGACGCATTT ATGTATTATG CTGCCAAGTT TAGGTGGGTA GGTTTATATG ATTGTTTTTA 6412 .......... .......... .......... .......... .......... .......... 119 TATATAGGTA AATCAACCAA ATCAAATTTT AAAAGAATCT TTAGAGCTTA TTACAAGGGA 6352 .......... .......... .......... .......... .......... .......... 119 TTTGTTAATG AGAATTTTTT AATCAAGAAG TTAATGAAGA AGTTCTGTTG TTGTTTCTGG 6292 .......... .......... .......... .......... .......... .......... 119 ATAGTTCTGT TTCTAATTTT GAAGCTCAAA ATGGATTTTT TAATGATTTT AATTTTCCTA 6232 .......... .......... .......... .......... .......... .......... 119 AAGGTTAAAT GGAATAGACT TTGAGGAGAT ACACATAAAT CTCTCCAAGC GCCAGCAGTT 6172 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GTTAAAT GGAATAGACT TTGAGGAGAT ACACATAAAT CTCTCCAAGC GCCAGCAGTT 176 ATCTCCTGAA TTTAAAGGTT TGTCTTTTTT ATTTACTCCA CTTTTCGCTA GATATTATTT 6112 |||||||||| ||||||| ATCTCCTGAA TTTAAAG... .......... .......... .......... .......... 193 ATTTCTTAGT TTCAAGATAA TTTCAAAAGT TTTCTGACTT CATCTTCAGA AATTAATCCC 6052 | |||||||||| .......... .......... .......... .......... .........A AATTAATCCC 204 ATGAAGCAAG TACCAGCTAT AATTGATGGA AGATTTAAGC TTTTCGAAAG GTAATATTCA 5992 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAAGCAAG TACCAGCTAT AATTGATGGA AGATTTAAGC TTTTCGAAAG .......... 254 GCTTGAACTG AACATTTTAG CATTTTCCAG TATCACAACT TTGTTAACGT CTATTATTCG 5932 .......... .......... .......... .......... .......... .......... 254 CTGTATATGT TGCATACATC CTAAAGCTTA TGATTTAGTC TTTCATTCTC GTAGAAGTCA 5872 .......... .......... .......... .......... .......... .......... 254 TGTTTGTAGC GTTCACCTAA TCACCTGTGC ATATATACTG CAAGAAGTGA AAAAATTTGT 5812 .......... .......... .......... .......... .......... .......... 254 AAAAATTTCT ATGTTTTGTG TACACCATGT GGGCACAAGT AACTACTGTT GAAAATGCCT 5752 .......... .......... .......... .......... .......... .......... 254 GTTGAGACGG GACAATTTTA TCTTTTTCTG AGTTTCACAT AAAGTATTTT ATAACTTGGA 5692 .......... .......... .......... .......... .......... .......... 254 TAATGTTTAA AATTAACTAA CTGATTATAT GTCCTCGCAG TCATGCAATT CTTAGATATC 5632 |||||||||| |||||||||| .......... .......... .......... .......... TCATGCAATT CTTAGATATC 274 TGGCTTGTGC ATTTCCAGGA ATAGCAGATC ATTGGTAAGT ATCGACTTAT TTTGAGTATA 5572 |||||||||| |||||||||| |||||||||| |||| TGGCTTGTGC ATTTCCAGGA ATAGCAGATC ATTG...... .......... .......... 308 TACTGTAGTC AAATGCTTTG TATCATGGAA ATAAGTACTC TTTAAGCGCT TTTTCTAGAC 5512 .......... .......... .......... .......... .......... .......... 308 ATAGGATCCA ACCCGGACAG ATCAGGGATA TAAACTTGAT GGGTTCAACT TTTATATTCT 5452 .......... .......... .......... .......... .......... .......... 308 CACTGCTGGT GCAATCACGT GTATACCTAA GTTCCATGTC AAAATGTTGG GTTAAGCAGA 5392 .......... .......... .......... .......... .......... .......... 308 ACCCATATTG CATCTGCTGC TGAACATGGC AATCGGCATT ACGAATGGAA TTATTCTGCA 5332 .......... .......... .......... .......... .......... .......... 308 GTTGGCATCT GTGTGAATTT AATGCCACAT GTACCTGTTA TTTGATTGAT AATTGTTTGT 5272 .......... .......... .......... .......... .......... .......... 308 TGTTTTTCTA ACTTGGAGTT CAACTAGTAG AGTTTTGATA AGAAACGCCA TCGGGTTTTG 5212 .......... .......... .......... .......... .......... .......... 308 CTAAGAAACC CGTCATTTGT ACCAAAACCG AGAATAACAT GACATGTGTC TTCTGCATAC 5152 .......... .......... .......... .......... .......... .......... 308 ACTTGTCACC TAACTATTGT TAATTAATGT GCATTTTCAT CTCATTTATT ACTTAAGAAT 5092 .......... .......... .......... .......... .......... .......... 308 GGTAAAATAG TGAACGAACC AAAGGAGAAC TCCCAACAGA AAAGAAGAAC AAGTAACATA 5032 .......... .......... .......... .......... .......... .......... 308 TTAGCAGATT GGAAAATACC AGCTCAACAA ATGAAGTATC AGCACCAAAT AACAAACAAG 4972 .......... .......... .......... .......... .......... .......... 308 CCCTATCAAA ATTTGAGAAG AGATGACTAT TGAACACAGA AGTCATCCTT TGGAATCTCT 4912 .......... .......... .......... .......... .......... .......... 308 GTACTATACC AACTTATCCG AAAAAAGAAA AAAAAAGTAC ATCTATGGTG CCTATGAACA 4852 .......... .......... .......... .......... .......... .......... 308 ATGCTGCTCT TCATTCTTAG TGATAGACTA ATGTACACAC TCTTAGGTTG TATAAATTCT 4792 .......... .......... .......... .......... .......... .......... 308 TATCCTGGTT AGTGTTTGAT TTAAATCCTC TTTCTTACAC TTTCCTATTG AAGGTACCCT 4732 ||||||| .......... .......... .......... .......... .......... ...GTACCCT 315 GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 4672 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 375 CGTCGCGGTG CAGGTGCTTA TATCAGATAT TGAGATCATT TCAGTTTCCG GTTGAATTAT 4612 |||||||||| ||| CGTCGCGGTG CAG....... .......... .......... .......... .......... 388 GATTACTTTA CGTTGACCTT ACTTGAGTTA ATTTAGTGGT GAATGCTGGG ATATGCAAGA 4552 .......... .......... .......... .......... .......... .......... 388 ACTTGGGCAA AAAGATGCAT ACAGGCTACA GTTAAGGCAA TGACTGGATT AAAATTTTAT 4492 .......... .......... .......... .......... .......... .......... 388 CCCAAATAAG ATTAATCAAG GAAATCTGTT TGTATGATCA GTTTTAAAAA AGAGTATCTG 4432 .......... .......... .......... .......... .......... .......... 388 CTTGTCATGA TGCTCTTTGC TAAGATTCTC GCTACTAACC TGATACAAGA GAGTTGCTGC 4372 .......... .......... .......... .......... .......... .......... 388 AAGCCTACAA GTTTCTTAGT CTATCTTTAG GACTTGATTC CTCTAATTGA AACACCTGAC 4312 .......... .......... .......... .......... .......... .......... 388 CTTTCTCAAA CCTCTAATTG TGAGTCTAGC TACTGCATGA TGGTGACTTC TTTTCTTCAA 4252 .......... .......... .......... .......... .......... .......... 388 CAATAACAGT GACTAAAACC ACTAGGATAG GTAGTCTTTT CACCAATGTT CTATAATTAC 4192 .......... .......... .......... .......... .......... .......... 388 TACTTCTTGT TGATAACTTA TTATGCCACT CTTTATCTGT TTCCAACAGT TTTCTGAAGC 4132 .......... .......... .......... .......... .......... .......... 388 AGTACTTATG TTTTCAGCTG GATATATCTT TAATACTGTT CTTGCTCCTG CTTTTGGGCT 4072 ||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .......CTG GATATATCTT TAATACTGTT CTTGCTCCTG CTTTTGGGCT 431 GCCATTGAAT CCACAAGCAG CAGCAGAAGC TGAGAAAGTC CTTTTGGCAT CTCTTGCAAA 4012 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCCATTGAAT CCACAAGCAG CAGCAGAAGC TGAGAAAGTC CTTTTGGCAT CTCTTGCAAA 491 AGTCGAGTCT GTTTGGCTCC AGAGAAAAGG ACGGTTTTTG CTTGGGAGTG GCCAACCTTC 3952 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTCGAGTCT GTTTGGCTCC AGAGAAAAGG ACGGTTTTTG CTTGGGAGTG GCCAACCTTC 551 AATTGCAGAT CTTAGCTTAG TGTGTGAGAT TATGGAACTT GAGGTAAAGA GATTTTCCCT 3892 |||||||||| |||||||||| |||||||||| |||||||||| ||| AATTGCAGAT CTTAGCTTAG TGTGTGAGAT TATGGAACTT GAG....... .......... 594 TTTCCTGCTG TTTCTTTACA TCTTTTGATC CAGAACATAA ATTACCAAGA GGAGTCTTGT 3832 .......... .......... .......... .......... .......... .......... 594 TGAAATTTCT TTTATAATGA ATTTGGGTTT TGGATGTGAT GATTTTAGAT TTTGGATGAG 3772 || |||||||||| .......... .......... .......... .......... ........AT TTTGGATGAG 606 AAGGATCGTG AGCGGATAAT AGGCCCATAC AAGAGAGTTT TGAAGTGGAT TGATGACACA 3712 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGATCGTG AGCGGATAAT AGGCCCATAC AAGAGAGTTT TGAAGTGGAT TGATGACACA 666 AAGAATGCCA TGGAACCTCA TT 3690 ||||||||| |||||||||| || NAGAATGCCA TGGAACCTCA TT 688 hqPGS_C08HBa0251H04.1-_SGN-E373791+ (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3690) ******************************************************************************** EST sequence 1 -strand 349 n (File: SGN-E237034-) 1 CAAGGATCCA GGTGCTGCAT TGTGCTCGTA GAATCACCGT GCACCATGGC AGATAAACAG 61 TGTGTATGCA AAAGTTGATA CAGGAAGGAG AGTTCTGATC CAAGCTTTCC TTACTCATTT 121 TCTCCATGTA TATGTACTTT ATAAGTTTCT ATCAGTTGTT AATGTAGTCC ACAACATATG 181 TAATCGAACA AACTGAGAGT CCCTTGCTCT TGTGCTTGTA ACTTCAGAGC TTATGAACAC 241 ATTTTTGTCT ACAATGTAAT CATTAAAGAG TCTTGGTTAT ACGGAGATTA TTCTCTTGAT 301 GTTAATGGAG GAAAATTTGT TGTTTTTCTG GAAAAAAAAA AAAAAAAAA Predicted gene structure (within gDNA segment 4172 to 2461): Exon 1 3562 3232 ( 331 n); cDNA 1 331 ( 331 n); score: 0.997 Intron 1 3231 3064 ( 168 n); Pd: 0.000 (s: 1.00), Pa: 0.315 (s: 0) Exon 2 3063 3048 ( 16 n); cDNA 332 347 ( 16 n); score: 0.688 MATCH C08HBa0251H04.1- SGN-E237034- 0.997 347 0.994 C PGS_C08HBa0251H04.1-_SGN-E237034- (3562 3232,3063 3048) Alignment (genomic DNA sequence = upper lines): CAAGGATCCA GGTGCTGCAT TGTGCTCGTA GTATCACCGT GCACCATGGC AGATAAACAG 3503 |||||||||| |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| CAAGGATCCA GGTGCTGCAT TGTGCTCGTA GAATCACCGT GCACCATGGC AGATAAACAG 60 TGTGTATGCA AAAGTTGATA CAGGAAGGAG AGTTCTGATC CAAGCTTTCC TTACTCATTT 3443 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGTATGCA AAAGTTGATA CAGGAAGGAG AGTTCTGATC CAAGCTTTCC TTACTCATTT 120 TCTCCATGTA TATGTACTTT ATAAGTTTCT ATCAGTTGTT AATGTAGTCC ACAACATATG 3383 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCCATGTA TATGTACTTT ATAAGTTTCT ATCAGTTGTT AATGTAGTCC ACAACATATG 180 TAATCGAACA AACTGAGAGT CCCTTGCTCT TGTGCTTGTA ACTTCAGAGC TTATGAACAC 3323 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATCGAACA AACTGAGAGT CCCTTGCTCT TGTGCTTGTA ACTTCAGAGC TTATGAACAC 240 ATTTTTGTCT ACAATGTAAT CATTAAAGAG TCTTGGTTAT ACGGAGATTA TTCTCTTGAT 3263 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTTTGTCT ACAATGTAAT CATTAAAGAG TCTTGGTTAT ACGGAGATTA TTCTCTTGAT 300 GTTAATGGAG GAAAATTTGT TGTTTTTCTG GATATTCTGT TTCTAGTTTT GAAGCTCAAA 3203 |||||||||| |||||||||| |||||||||| | GTTAATGGAG GAAAATTTGT TGTTTTTCTG G......... .......... .......... 331 ATGGAGTTTT CAATGATTAA TTTTCCAAAA GGTTAAATGG AATAGAATTT GAGGAGGTAA 3143 .......... .......... .......... .......... .......... .......... 331 GTACAGAGCT CTTCAAGCAT CAGCAGTTGT CTCCTGATTT TGAAGGTTTG TCTTTTTTCA 3083 .......... .......... .......... .......... .......... .......... 331 TATAAACTCT ACATTTCAGT AGAAAAATGG AAAAA 3048 | ||||| ||||| .......... .........A AAAAAAAAAA AAAAA 347 hqPGS_C08HBa0251H04.1-_SGN-E237034- (3562 3232) ******************************************************************************** EST sequence 2 -strand 344 n (File: SGN-E237429-) 1 ATCCAGGTGC TGCATTGTGC TCGTAGTATC ACCGTGCACC ATGGCAGATA AACAGTGTGT 61 ATGCAAAAGT TGATACAGGA AGGAGAGTTC TGATCCAAGC TTTCCTTACT CATTTTCTCC 121 ATGTATATGT ACTTTATAAG TTTCTATCAG TTGTTAATGT AGTCCACAAC ATATGTAATC 181 GAACAAACTG AGAGTCCCTT GCTCTTGTGC TTGTAACTTC AGAGCTTATG AACACATTTT 241 TGTCTACAAT GTAATCATTA AAGAGTCTTG GTTATACGGA GATTATTCTC TTGATGTTAA 301 TGGAGGAAAA TTTGTTGTTT TTCTGGAAAA AAAAAAAAAA AAAA Predicted gene structure (within gDNA segment 4167 to 2461): Exon 1 3557 3232 ( 326 n); cDNA 1 326 ( 326 n); score: 1.000 Intron 1 3231 3064 ( 168 n); Pd: 0.000 (s: 1.00), Pa: 0.315 (s: 0) Exon 2 3063 3048 ( 16 n); cDNA 327 342 ( 16 n); score: 0.688 MATCH C08HBa0251H04.1- SGN-E237429- 1.000 342 0.994 C PGS_C08HBa0251H04.1-_SGN-E237429- (3557 3232,3063 3048) Alignment (genomic DNA sequence = upper lines): ATCCAGGTGC TGCATTGTGC TCGTAGTATC ACCGTGCACC ATGGCAGATA AACAGTGTGT 3498 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCCAGGTGC TGCATTGTGC TCGTAGTATC ACCGTGCACC ATGGCAGATA AACAGTGTGT 60 ATGCAAAAGT TGATACAGGA AGGAGAGTTC TGATCCAAGC TTTCCTTACT CATTTTCTCC 3438 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGCAAAAGT TGATACAGGA AGGAGAGTTC TGATCCAAGC TTTCCTTACT CATTTTCTCC 120 ATGTATATGT ACTTTATAAG TTTCTATCAG TTGTTAATGT AGTCCACAAC ATATGTAATC 3378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTATATGT ACTTTATAAG TTTCTATCAG TTGTTAATGT AGTCCACAAC ATATGTAATC 180 GAACAAACTG AGAGTCCCTT GCTCTTGTGC TTGTAACTTC AGAGCTTATG AACACATTTT 3318 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAACAAACTG AGAGTCCCTT GCTCTTGTGC TTGTAACTTC AGAGCTTATG AACACATTTT 240 TGTCTACAAT GTAATCATTA AAGAGTCTTG GTTATACGGA GATTATTCTC TTGATGTTAA 3258 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTCTACAAT GTAATCATTA AAGAGTCTTG GTTATACGGA GATTATTCTC TTGATGTTAA 300 TGGAGGAAAA TTTGTTGTTT TTCTGGATAT TCTGTTTCTA GTTTTGAAGC TCAAAATGGA 3198 |||||||||| |||||||||| |||||| TGGAGGAAAA TTTGTTGTTT TTCTGG.... .......... .......... .......... 326 GTTTTCAATG ATTAATTTTC CAAAAGGTTA AATGGAATAG AATTTGAGGA GGTAAGTACA 3138 .......... .......... .......... .......... .......... .......... 326 GAGCTCTTCA AGCATCAGCA GTTGTCTCCT GATTTTGAAG GTTTGTCTTT TTTCATATAA 3078 .......... .......... .......... .......... .......... .......... 326 ACTCTACATT TCAGTAGAAA AATGGAAAAA 3048 | ||| || ||||| .......... ....AAAAAA AAAAAAAAAA 342 hqPGS_C08HBa0251H04.1-_SGN-E237429- (3557 3232) ******************************************************************************** EST sequence 79 +strand 598 n (File: SGN-E292336+) 1 GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 61 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 121 TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA GCAGTTATCT 181 CCTGAATTTA AAGAAATTAA TCCCATGAAG CAAGTACCAG CTATAATTGA TGGAAGATTT 241 AAGCTTTTCG AAAGTCATGC AATTCTTAGA TATCTGGCTT GTGCATTTCC AGGAATAGCA 301 GATCATTGGT ACCCTGCTGA CTTATACAAA AGAGCAAAGG TAGACTCTGT GTTGGACTGG 361 CATCACTCAA ACTTGCGTCG CGGTGCACCT GGATATATCT TTAATACTGA TCTTGCTCCT 421 GCTTTTGGGC TGCCATTGAA TCCACAAGCA GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA 481 TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC CAGAGAAAAC GACGGTTTTT GCTTGGGAGT 541 GGCCAACCTT CAATTGCAGA GCTTAGCTTA GTGTGTGAGA TTATGGAACT TGAGATTT Predicted gene structure (within gDNA segment 7791 to 3259): Exon 1 7191 7073 ( 119 n); cDNA 1 119 ( 119 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 120 193 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 194 254 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 255 308 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 309 388 ( 80 n); score: 0.988 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 0.98), Pa: 0.096 (s: 0.98) Exon 6 4114 3909 ( 206 n); cDNA 389 594 ( 206 n); score: 0.985 MATCH C08HBa0251H04.1- SGN-E292336+ 0.993 594 0.993 C PGS_C08HBa0251H04.1-_SGN-E292336+ (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909) Alignment (genomic DNA sequence = upper lines): GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 7132 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 60 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 7072 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAA. 119 TAAGTATTTT TCATTTTTCT TTTCTCGAGA TGTTTAAAGC TCGTAATGGA GGTTTTAATG 7012 .......... .......... .......... .......... .......... .......... 119 AATTATTTTC TAGAAATTTG ATTAACTCCG TTAGATAGGA ACAGATGATA AGTACTGAGT 6952 .......... .......... .......... .......... .......... .......... 119 ATTAGAACGG AAAGATCTAT TAGATAATGA GCTATTGACA CTATTCATCA ACAATTCGTA 6892 .......... .......... .......... .......... .......... .......... 119 GTGCTATGTT AATTCTATAT GTAACTTTGA ATCTCAAAAT GGAGTTTTTA GTGACTGATC 6832 .......... .......... .......... .......... .......... .......... 119 TTTCAAAAAG TTTTTTTTGT TTTCTTTGAA TCCTCATATT TGTAGCTTTA GAAGTTACGA 6772 .......... .......... .......... .......... .......... .......... 119 ATACAATAAC ATCAAGAACA TCCATAACGT AGTTGTTAAA AGACTCTCCG TTAGGCGAAA 6712 .......... .......... .......... .......... .......... .......... 119 TTTATTGACA TAAATATGCC CTTTAACTTG ACTTTAAATT ACATTTATGT CCTTCAACTT 6652 .......... .......... .......... .......... .......... .......... 119 TGGGTGTGGA CAAGTATAAA TTTGTATAAA GTTGAATAAA TAGACACGTC ATTTTTTGTC 6592 .......... .......... .......... .......... .......... .......... 119 CTACGTTATT GTGCCACGTA GGACTCATGT GTGTACTTGT TCAAATTTAT ACAAGTTTGA 6532 .......... .......... .......... .......... .......... .......... 119 GTATCTACTT GTGCATATCC ATAATAGAAG GACATAAATG TTAAATGAGA CCAAGTTAAA 6472 .......... .......... .......... .......... .......... .......... 119 TGACGCATTT ATGTATTATG CTGCCAAGTT TAGGTGGGTA GGTTTATATG ATTGTTTTTA 6412 .......... .......... .......... .......... .......... .......... 119 TATATAGGTA AATCAACCAA ATCAAATTTT AAAAGAATCT TTAGAGCTTA TTACAAGGGA 6352 .......... .......... .......... .......... .......... .......... 119 TTTGTTAATG AGAATTTTTT AATCAAGAAG TTAATGAAGA AGTTCTGTTG TTGTTTCTGG 6292 .......... .......... .......... .......... .......... .......... 119 ATAGTTCTGT TTCTAATTTT GAAGCTCAAA ATGGATTTTT TAATGATTTT AATTTTCCTA 6232 .......... .......... .......... .......... .......... .......... 119 AAGGTTAAAT GGAATAGACT TTGAGGAGAT ACACATAAAT CTCTCCAAGC GCCAGCAGTT 6172 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GTTAAAT GGAATAGACT TTGAGGAGAT ACACATAAAT CTCTCCAAGC GCCAGCAGTT 176 ATCTCCTGAA TTTAAAGGTT TGTCTTTTTT ATTTACTCCA CTTTTCGCTA GATATTATTT 6112 |||||||||| ||||||| ATCTCCTGAA TTTAAAG... .......... .......... .......... .......... 193 ATTTCTTAGT TTCAAGATAA TTTCAAAAGT TTTCTGACTT CATCTTCAGA AATTAATCCC 6052 | |||||||||| .......... .......... .......... .......... .........A AATTAATCCC 204 ATGAAGCAAG TACCAGCTAT AATTGATGGA AGATTTAAGC TTTTCGAAAG GTAATATTCA 5992 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAAGCAAG TACCAGCTAT AATTGATGGA AGATTTAAGC TTTTCGAAAG .......... 254 GCTTGAACTG AACATTTTAG CATTTTCCAG TATCACAACT TTGTTAACGT CTATTATTCG 5932 .......... .......... .......... .......... .......... .......... 254 CTGTATATGT TGCATACATC CTAAAGCTTA TGATTTAGTC TTTCATTCTC GTAGAAGTCA 5872 .......... .......... .......... .......... .......... .......... 254 TGTTTGTAGC GTTCACCTAA TCACCTGTGC ATATATACTG CAAGAAGTGA AAAAATTTGT 5812 .......... .......... .......... .......... .......... .......... 254 AAAAATTTCT ATGTTTTGTG TACACCATGT GGGCACAAGT AACTACTGTT GAAAATGCCT 5752 .......... .......... .......... .......... .......... .......... 254 GTTGAGACGG GACAATTTTA TCTTTTTCTG AGTTTCACAT AAAGTATTTT ATAACTTGGA 5692 .......... .......... .......... .......... .......... .......... 254 TAATGTTTAA AATTAACTAA CTGATTATAT GTCCTCGCAG TCATGCAATT CTTAGATATC 5632 |||||||||| |||||||||| .......... .......... .......... .......... TCATGCAATT CTTAGATATC 274 TGGCTTGTGC ATTTCCAGGA ATAGCAGATC ATTGGTAAGT ATCGACTTAT TTTGAGTATA 5572 |||||||||| |||||||||| |||||||||| |||| TGGCTTGTGC ATTTCCAGGA ATAGCAGATC ATTG...... .......... .......... 308 TACTGTAGTC AAATGCTTTG TATCATGGAA ATAAGTACTC TTTAAGCGCT TTTTCTAGAC 5512 .......... .......... .......... .......... .......... .......... 308 ATAGGATCCA ACCCGGACAG ATCAGGGATA TAAACTTGAT GGGTTCAACT TTTATATTCT 5452 .......... .......... .......... .......... .......... .......... 308 CACTGCTGGT GCAATCACGT GTATACCTAA GTTCCATGTC AAAATGTTGG GTTAAGCAGA 5392 .......... .......... .......... .......... .......... .......... 308 ACCCATATTG CATCTGCTGC TGAACATGGC AATCGGCATT ACGAATGGAA TTATTCTGCA 5332 .......... .......... .......... .......... .......... .......... 308 GTTGGCATCT GTGTGAATTT AATGCCACAT GTACCTGTTA TTTGATTGAT AATTGTTTGT 5272 .......... .......... .......... .......... .......... .......... 308 TGTTTTTCTA ACTTGGAGTT CAACTAGTAG AGTTTTGATA AGAAACGCCA TCGGGTTTTG 5212 .......... .......... .......... .......... .......... .......... 308 CTAAGAAACC CGTCATTTGT ACCAAAACCG AGAATAACAT GACATGTGTC TTCTGCATAC 5152 .......... .......... .......... .......... .......... .......... 308 ACTTGTCACC TAACTATTGT TAATTAATGT GCATTTTCAT CTCATTTATT ACTTAAGAAT 5092 .......... .......... .......... .......... .......... .......... 308 GGTAAAATAG TGAACGAACC AAAGGAGAAC TCCCAACAGA AAAGAAGAAC AAGTAACATA 5032 .......... .......... .......... .......... .......... .......... 308 TTAGCAGATT GGAAAATACC AGCTCAACAA ATGAAGTATC AGCACCAAAT AACAAACAAG 4972 .......... .......... .......... .......... .......... .......... 308 CCCTATCAAA ATTTGAGAAG AGATGACTAT TGAACACAGA AGTCATCCTT TGGAATCTCT 4912 .......... .......... .......... .......... .......... .......... 308 GTACTATACC AACTTATCCG AAAAAAGAAA AAAAAAGTAC ATCTATGGTG CCTATGAACA 4852 .......... .......... .......... .......... .......... .......... 308 ATGCTGCTCT TCATTCTTAG TGATAGACTA ATGTACACAC TCTTAGGTTG TATAAATTCT 4792 .......... .......... .......... .......... .......... .......... 308 TATCCTGGTT AGTGTTTGAT TTAAATCCTC TTTCTTACAC TTTCCTATTG AAGGTACCCT 4732 ||||||| .......... .......... .......... .......... .......... ...GTACCCT 315 GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 4672 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 375 CGTCGCGGTG CAGGTGCTTA TATCAGATAT TGAGATCATT TCAGTTTCCG GTTGAATTAT 4612 |||||||||| || CGTCGCGGTG CAC....... .......... .......... .......... .......... 388 GATTACTTTA CGTTGACCTT ACTTGAGTTA ATTTAGTGGT GAATGCTGGG ATATGCAAGA 4552 .......... .......... .......... .......... .......... .......... 388 ACTTGGGCAA AAAGATGCAT ACAGGCTACA GTTAAGGCAA TGACTGGATT AAAATTTTAT 4492 .......... .......... .......... .......... .......... .......... 388 CCCAAATAAG ATTAATCAAG GAAATCTGTT TGTATGATCA GTTTTAAAAA AGAGTATCTG 4432 .......... .......... .......... .......... .......... .......... 388 CTTGTCATGA TGCTCTTTGC TAAGATTCTC GCTACTAACC TGATACAAGA GAGTTGCTGC 4372 .......... .......... .......... .......... .......... .......... 388 AAGCCTACAA GTTTCTTAGT CTATCTTTAG GACTTGATTC CTCTAATTGA AACACCTGAC 4312 .......... .......... .......... .......... .......... .......... 388 CTTTCTCAAA CCTCTAATTG TGAGTCTAGC TACTGCATGA TGGTGACTTC TTTTCTTCAA 4252 .......... .......... .......... .......... .......... .......... 388 CAATAACAGT GACTAAAACC ACTAGGATAG GTAGTCTTTT CACCAATGTT CTATAATTAC 4192 .......... .......... .......... .......... .......... .......... 388 TACTTCTTGT TGATAACTTA TTATGCCACT CTTTATCTGT TTCCAACAGT TTTCTGAAGC 4132 .......... .......... .......... .......... .......... .......... 388 AGTACTTATG TTTTCAGCTG GATATATCTT TAATACTGTT CTTGCTCCTG CTTTTGGGCT 4072 ||| |||||||||| |||||||| | |||||||||| |||||||||| .......... .......CTG GATATATCTT TAATACTGAT CTTGCTCCTG CTTTTGGGCT 431 GCCATTGAAT CCACAAGCAG CAGCAGAAGC TGAGAAAGTC CTTTTGGCAT CTCTTGCAAA 4012 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCCATTGAAT CCACAAGCAG CAGCAGAAGC TGAGAAAGTC CTTTTGGCAT CTCTTGCAAA 491 AGTCGAGTCT GTTTGGCTCC AGAGAAAAGG ACGGTTTTTG CTTGGGAGTG GCCAACCTTC 3952 |||||||||| |||||||||| |||||||| | |||||||||| |||||||||| |||||||||| AGTCGAGTCT GTTTGGCTCC AGAGAAAACG ACGGTTTTTG CTTGGGAGTG GCCAACCTTC 551 AATTGCAGAT CTTAGCTTAG TGTGTGAGAT TATGGAACTT GAG 3909 ||||||||| |||||||||| |||||||||| |||||||||| ||| AATTGCAGAG CTTAGCTTAG TGTGTGAGAT TATGGAACTT GAG 594 hqPGS_C08HBa0251H04.1-_SGN-E292336+ (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909) ******************************************************************************** EST sequence 129 +strand 512 n (File: SGN-E312517+) 1 GCTCGCGTAG CCGGCGGTGA GTTCGTCGGA GTTAATTTCC AGTGAGAATA GAATTGATTG 61 AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 121 ATCTTCTGCA AGTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 181 CAGCAGTTAT CTCCTGAATT TAAAGAAATT AATCCCATGA AGCAAGTACC AGCTATAATT 241 GATGGAAGAT TTAAGCTTTT CGAAAGTCAT GCAATTCTTA GATATCTGGC TTGTGCATTT 301 CCAGGAATAG CAGATCATTG GTACCCTGCT GACTTATACA AAAGAGCAAA GGTAGACTCT 361 GTGTTGGACT GGCATCACTC AAACTTGCGT CGCGGTGCAG CTGGATATAT CTTTAATACT 421 GTTCTTGCTC CTGCTTTTGG GCTGCCATTG AATCCACAAG CAGCAGCAGA AGCTGAGAAA 481 GTCCTTTTGG CATCTCTTGC AAAAGTCGAG TC Predicted gene structure (within gDNA segment 7803 to 3393): Exon 1 7203 7073 ( 131 n); cDNA 1 131 ( 131 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 132 205 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 206 266 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 267 320 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 321 400 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 4003 ( 112 n); cDNA 401 512 ( 112 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E312517+ 1.000 512 1.000 C PGS_C08HBa0251H04.1-_SGN-E312517+ (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4003) Alignment (genomic DNA sequence = upper lines): GCTCGCGTAG CCGGCGGTGA GTTCGTCGGA GTTAATTTCC AGTGAGAATA GAATTGATTG 7144 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTCGCGTAG CCGGCGGTGA GTTCGTCGGA GTTAATTTCC AGTGAGAATA GAATTGATTG 60 AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 7084 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 120 ATCTTCTGCA AGTAAGTATT TTTCATTTTT CTTTTCTCGA GATGTTTAAA GCTCGTAATG 7024 |||||||||| | ATCTTCTGCA A......... .......... .......... .......... .......... 131 GAGGTTTTAA TGAATTATTT TCTAGAAATT TGATTAACTC CGTTAGATAG GAACAGATGA 6964 .......... .......... .......... .......... .......... .......... 131 TAAGTACTGA GTATTAGAAC GGAAAGATCT ATTAGATAAT GAGCTATTGA CACTATTCAT 6904 .......... .......... .......... .......... .......... .......... 131 CAACAATTCG TAGTGCTATG TTAATTCTAT ATGTAACTTT GAATCTCAAA ATGGAGTTTT 6844 .......... .......... .......... .......... .......... .......... 131 TAGTGACTGA TCTTTCAAAA AGTTTTTTTT GTTTTCTTTG AATCCTCATA TTTGTAGCTT 6784 .......... .......... .......... .......... .......... .......... 131 TAGAAGTTAC GAATACAATA ACATCAAGAA CATCCATAAC GTAGTTGTTA AAAGACTCTC 6724 .......... .......... .......... .......... .......... .......... 131 CGTTAGGCGA AATTTATTGA CATAAATATG CCCTTTAACT TGACTTTAAA TTACATTTAT 6664 .......... .......... .......... .......... .......... .......... 131 GTCCTTCAAC TTTGGGTGTG GACAAGTATA AATTTGTATA AAGTTGAATA AATAGACACG 6604 .......... .......... .......... .......... .......... .......... 131 TCATTTTTTG TCCTACGTTA TTGTGCCACG TAGGACTCAT GTGTGTACTT GTTCAAATTT 6544 .......... .......... .......... .......... .......... .......... 131 ATACAAGTTT GAGTATCTAC TTGTGCATAT CCATAATAGA AGGACATAAA TGTTAAATGA 6484 .......... .......... .......... .......... .......... .......... 131 GACCAAGTTA AATGACGCAT TTATGTATTA TGCTGCCAAG TTTAGGTGGG TAGGTTTATA 6424 .......... .......... .......... .......... .......... .......... 131 TGATTGTTTT TATATATAGG TAAATCAACC AAATCAAATT TTAAAAGAAT CTTTAGAGCT 6364 .......... .......... .......... .......... .......... .......... 131 TATTACAAGG GATTTGTTAA TGAGAATTTT TTAATCAAGA AGTTAATGAA GAAGTTCTGT 6304 .......... .......... .......... .......... .......... .......... 131 TGTTGTTTCT GGATAGTTCT GTTTCTAATT TTGAAGCTCA AAATGGATTT TTTAATGATT 6244 .......... .......... .......... .......... .......... .......... 131 TTAATTTTCC TAAAGGTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA 6184 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA 176 GCGCCAGCAG TTATCTCCTG AATTTAAAGG TTTGTCTTTT TTATTTACTC CACTTTTCGC 6124 |||||||||| |||||||||| ||||||||| GCGCCAGCAG TTATCTCCTG AATTTAAAG. .......... .......... .......... 205 TAGATATTAT TTATTTCTTA GTTTCAAGAT AATTTCAAAA GTTTTCTGAC TTCATCTTCA 6064 .......... .......... .......... .......... .......... .......... 205 GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG GAAGATTTAA GCTTTTCGAA 6004 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .AAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG GAAGATTTAA GCTTTTCGAA 264 AGGTAATATT CAGCTTGAAC TGAACATTTT AGCATTTTCC AGTATCACAA CTTTGTTAAC 5944 || AG........ .......... .......... .......... .......... .......... 266 GTCTATTATT CGCTGTATAT GTTGCATACA TCCTAAAGCT TATGATTTAG TCTTTCATTC 5884 .......... .......... .......... .......... .......... .......... 266 TCGTAGAAGT CATGTTTGTA GCGTTCACCT AATCACCTGT GCATATATAC TGCAAGAAGT 5824 .......... .......... .......... .......... .......... .......... 266 GAAAAAATTT GTAAAAATTT CTATGTTTTG TGTACACCAT GTGGGCACAA GTAACTACTG 5764 .......... .......... .......... .......... .......... .......... 266 TTGAAAATGC CTGTTGAGAC GGGACAATTT TATCTTTTTC TGAGTTTCAC ATAAAGTATT 5704 .......... .......... .......... .......... .......... .......... 266 TTATAACTTG GATAATGTTT AAAATTAACT AACTGATTAT ATGTCCTCGC AGTCATGCAA 5644 |||||||| .......... .......... .......... .......... .......... ..TCATGCAA 274 TTCTTAGATA TCTGGCTTGT GCATTTCCAG GAATAGCAGA TCATTGGTAA GTATCGACTT 5584 |||||||||| |||||||||| |||||||||| |||||||||| |||||| TTCTTAGATA TCTGGCTTGT GCATTTCCAG GAATAGCAGA TCATTG.... .......... 320 ATTTTGAGTA TATACTGTAG TCAAATGCTT TGTATCATGG AAATAAGTAC TCTTTAAGCG 5524 .......... .......... .......... .......... .......... .......... 320 CTTTTTCTAG ACATAGGATC CAACCCGGAC AGATCAGGGA TATAAACTTG ATGGGTTCAA 5464 .......... .......... .......... .......... .......... .......... 320 CTTTTATATT CTCACTGCTG GTGCAATCAC GTGTATACCT AAGTTCCATG TCAAAATGTT 5404 .......... .......... .......... .......... .......... .......... 320 GGGTTAAGCA GAACCCATAT TGCATCTGCT GCTGAACATG GCAATCGGCA TTACGAATGG 5344 .......... .......... .......... .......... .......... .......... 320 AATTATTCTG CAGTTGGCAT CTGTGTGAAT TTAATGCCAC ATGTACCTGT TATTTGATTG 5284 .......... .......... .......... .......... .......... .......... 320 ATAATTGTTT GTTGTTTTTC TAACTTGGAG TTCAACTAGT AGAGTTTTGA TAAGAAACGC 5224 .......... .......... .......... .......... .......... .......... 320 CATCGGGTTT TGCTAAGAAA CCCGTCATTT GTACCAAAAC CGAGAATAAC ATGACATGTG 5164 .......... .......... .......... .......... .......... .......... 320 TCTTCTGCAT ACACTTGTCA CCTAACTATT GTTAATTAAT GTGCATTTTC ATCTCATTTA 5104 .......... .......... .......... .......... .......... .......... 320 TTACTTAAGA ATGGTAAAAT AGTGAACGAA CCAAAGGAGA ACTCCCAACA GAAAAGAAGA 5044 .......... .......... .......... .......... .......... .......... 320 ACAAGTAACA TATTAGCAGA TTGGAAAATA CCAGCTCAAC AAATGAAGTA TCAGCACCAA 4984 .......... .......... .......... .......... .......... .......... 320 ATAACAAACA AGCCCTATCA AAATTTGAGA AGAGATGACT ATTGAACACA GAAGTCATCC 4924 .......... .......... .......... .......... .......... .......... 320 TTTGGAATCT CTGTACTATA CCAACTTATC CGAAAAAAGA AAAAAAAAGT ACATCTATGG 4864 .......... .......... .......... .......... .......... .......... 320 TGCCTATGAA CAATGCTGCT CTTCATTCTT AGTGATAGAC TAATGTACAC ACTCTTAGGT 4804 .......... .......... .......... .......... .......... .......... 320 TGTATAAATT CTTATCCTGG TTAGTGTTTG ATTTAAATCC TCTTTCTTAC ACTTTCCTAT 4744 .......... .......... .......... .......... .......... .......... 320 TGAAGGTACC CTGCTGACTT ATACAAAAGA GCAAAGGTAG ACTCTGTGTT GGACTGGCAT 4684 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....GTACC CTGCTGACTT ATACAAAAGA GCAAAGGTAG ACTCTGTGTT GGACTGGCAT 375 CACTCAAACT TGCGTCGCGG TGCAGGTGCT TATATCAGAT ATTGAGATCA TTTCAGTTTC 4624 |||||||||| |||||||||| ||||| CACTCAAACT TGCGTCGCGG TGCAG..... .......... .......... .......... 400 CGGTTGAATT ATGATTACTT TACGTTGACC TTACTTGAGT TAATTTAGTG GTGAATGCTG 4564 .......... .......... .......... .......... .......... .......... 400 GGATATGCAA GAACTTGGGC AAAAAGATGC ATACAGGCTA CAGTTAAGGC AATGACTGGA 4504 .......... .......... .......... .......... .......... .......... 400 TTAAAATTTT ATCCCAAATA AGATTAATCA AGGAAATCTG TTTGTATGAT CAGTTTTAAA 4444 .......... .......... .......... .......... .......... .......... 400 AAAGAGTATC TGCTTGTCAT GATGCTCTTT GCTAAGATTC TCGCTACTAA CCTGATACAA 4384 .......... .......... .......... .......... .......... .......... 400 GAGAGTTGCT GCAAGCCTAC AAGTTTCTTA GTCTATCTTT AGGACTTGAT TCCTCTAATT 4324 .......... .......... .......... .......... .......... .......... 400 GAAACACCTG ACCTTTCTCA AACCTCTAAT TGTGAGTCTA GCTACTGCAT GATGGTGACT 4264 .......... .......... .......... .......... .......... .......... 400 TCTTTTCTTC AACAATAACA GTGACTAAAA CCACTAGGAT AGGTAGTCTT TTCACCAATG 4204 .......... .......... .......... .......... .......... .......... 400 TTCTATAATT ACTACTTCTT GTTGATAACT TATTATGCCA CTCTTTATCT GTTTCCAACA 4144 .......... .......... .......... .......... .......... .......... 400 GTTTTCTGAA GCAGTACTTA TGTTTTCAGC TGGATATATC TTTAATACTG TTCTTGCTCC 4084 | |||||||||| |||||||||| |||||||||| .......... .......... .........C TGGATATATC TTTAATACTG TTCTTGCTCC 431 TGCTTTTGGG CTGCCATTGA ATCCACAAGC AGCAGCAGAA GCTGAGAAAG TCCTTTTGGC 4024 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTTTTGGG CTGCCATTGA ATCCACAAGC AGCAGCAGAA GCTGAGAAAG TCCTTTTGGC 491 ATCTCTTGCA AAAGTCGAGT C 4003 |||||||||| |||||||||| | ATCTCTTGCA AAAGTCGAGT C 512 hqPGS_C08HBa0251H04.1-_SGN-E312517+ (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4003) ******************************************************************************** EST sequence 112 +strand 454 n (File: SGN-E288767+) 1 GGTGAGTTCG TCGGAGTTAA TTTCCAGTGA GAATAGAATT GATTGAAAGA GATGACGTTG 61 AAATTATACG TAGATCGTAT GTCCCAACCT TCTCGTGCAG TTATCATCTT CTGCAAGTTA 121 AATGGAATAG ACTTTGAGGA GATACACATA AATCTCTCCA AGCGCCAGCA GTTATCTCCT 181 GAATTTAAAG AAATTAATCC CATGAAGCAA GTACCAGCTA TAATTGATGG AAGATTTAAG 241 CTTTTCGAAA GTCATGCAAT TCTTAGATAT CTGGCTTGTG CATTTCCAGG AATAGCAGAT 301 CATTGGTACC CTGCTGACTT ATACAAAAGA GCAAAGGTAG ACTCTGTGTT GGACTGGCAT 361 CACTCAAACT TGCGTCGCGG TGCAGCTGGA TATATCTTTA ATACTGTTCT TGCTCCTGCT 421 TTTGGGCTGC CATTGAATCC ACAAGCAGCA GCAG Predicted gene structure (within gDNA segment 7788 to 3436): Exon 1 7188 7073 ( 116 n); cDNA 1 116 ( 116 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 117 190 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 1.00) Exon 3 6062 6002 ( 61 n); cDNA 191 251 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 252 305 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 4738 4659 ( 80 n); cDNA 306 385 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 1.00), Pa: 0.096 (s: 1.00) Exon 6 4114 4046 ( 69 n); cDNA 386 454 ( 69 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E288767+ 1.000 454 1.000 C PGS_C08HBa0251H04.1-_SGN-E288767+ (7188 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4046) Alignment (genomic DNA sequence = upper lines): GGTGAGTTCG TCGGAGTTAA TTTCCAGTGA GAATAGAATT GATTGAAAGA GATGACGTTG 7129 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTGAGTTCG TCGGAGTTAA TTTCCAGTGA GAATAGAATT GATTGAAAGA GATGACGTTG 60 AAATTATACG TAGATCGTAT GTCCCAACCT TCTCGTGCAG TTATCATCTT CTGCAAGTAA 7069 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| AAATTATACG TAGATCGTAT GTCCCAACCT TCTCGTGCAG TTATCATCTT CTGCAA.... 116 GTATTTTTCA TTTTTCTTTT CTCGAGATGT TTAAAGCTCG TAATGGAGGT TTTAATGAAT 7009 .......... .......... .......... .......... .......... .......... 116 TATTTTCTAG AAATTTGATT AACTCCGTTA GATAGGAACA GATGATAAGT ACTGAGTATT 6949 .......... .......... .......... .......... .......... .......... 116 AGAACGGAAA GATCTATTAG ATAATGAGCT ATTGACACTA TTCATCAACA ATTCGTAGTG 6889 .......... .......... .......... .......... .......... .......... 116 CTATGTTAAT TCTATATGTA ACTTTGAATC TCAAAATGGA GTTTTTAGTG ACTGATCTTT 6829 .......... .......... .......... .......... .......... .......... 116 CAAAAAGTTT TTTTTGTTTT CTTTGAATCC TCATATTTGT AGCTTTAGAA GTTACGAATA 6769 .......... .......... .......... .......... .......... .......... 116 CAATAACATC AAGAACATCC ATAACGTAGT TGTTAAAAGA CTCTCCGTTA GGCGAAATTT 6709 .......... .......... .......... .......... .......... .......... 116 ATTGACATAA ATATGCCCTT TAACTTGACT TTAAATTACA TTTATGTCCT TCAACTTTGG 6649 .......... .......... .......... .......... .......... .......... 116 GTGTGGACAA GTATAAATTT GTATAAAGTT GAATAAATAG ACACGTCATT TTTTGTCCTA 6589 .......... .......... .......... .......... .......... .......... 116 CGTTATTGTG CCACGTAGGA CTCATGTGTG TACTTGTTCA AATTTATACA AGTTTGAGTA 6529 .......... .......... .......... .......... .......... .......... 116 TCTACTTGTG CATATCCATA ATAGAAGGAC ATAAATGTTA AATGAGACCA AGTTAAATGA 6469 .......... .......... .......... .......... .......... .......... 116 CGCATTTATG TATTATGCTG CCAAGTTTAG GTGGGTAGGT TTATATGATT GTTTTTATAT 6409 .......... .......... .......... .......... .......... .......... 116 ATAGGTAAAT CAACCAAATC AAATTTTAAA AGAATCTTTA GAGCTTATTA CAAGGGATTT 6349 .......... .......... .......... .......... .......... .......... 116 GTTAATGAGA ATTTTTTAAT CAAGAAGTTA ATGAAGAAGT TCTGTTGTTG TTTCTGGATA 6289 .......... .......... .......... .......... .......... .......... 116 GTTCTGTTTC TAATTTTGAA GCTCAAAATG GATTTTTTAA TGATTTTAAT TTTCCTAAAG 6229 .......... .......... .......... .......... .......... .......... 116 GTTAAATGGA ATAGACTTTG AGGAGATACA CATAAATCTC TCCAAGCGCC AGCAGTTATC 6169 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTAAATGGA ATAGACTTTG AGGAGATACA CATAAATCTC TCCAAGCGCC AGCAGTTATC 176 TCCTGAATTT AAAGGTTTGT CTTTTTTATT TACTCCACTT TTCGCTAGAT ATTATTTATT 6109 |||||||||| |||| TCCTGAATTT AAAG...... .......... .......... .......... .......... 190 TCTTAGTTTC AAGATAATTT CAAAAGTTTT CTGACTTCAT CTTCAGAAAT TAATCCCATG 6049 |||| |||||||||| .......... .......... .......... .......... ......AAAT TAATCCCATG 204 AAGCAAGTAC CAGCTATAAT TGATGGAAGA TTTAAGCTTT TCGAAAGGTA ATATTCAGCT 5989 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| AAGCAAGTAC CAGCTATAAT TGATGGAAGA TTTAAGCTTT TCGAAAG... .......... 251 TGAACTGAAC ATTTTAGCAT TTTCCAGTAT CACAACTTTG TTAACGTCTA TTATTCGCTG 5929 .......... .......... .......... .......... .......... .......... 251 TATATGTTGC ATACATCCTA AAGCTTATGA TTTAGTCTTT CATTCTCGTA GAAGTCATGT 5869 .......... .......... .......... .......... .......... .......... 251 TTGTAGCGTT CACCTAATCA CCTGTGCATA TATACTGCAA GAAGTGAAAA AATTTGTAAA 5809 .......... .......... .......... .......... .......... .......... 251 AATTTCTATG TTTTGTGTAC ACCATGTGGG CACAAGTAAC TACTGTTGAA AATGCCTGTT 5749 .......... .......... .......... .......... .......... .......... 251 GAGACGGGAC AATTTTATCT TTTTCTGAGT TTCACATAAA GTATTTTATA ACTTGGATAA 5689 .......... .......... .......... .......... .......... .......... 251 TGTTTAAAAT TAACTAACTG ATTATATGTC CTCGCAGTCA TGCAATTCTT AGATATCTGG 5629 ||| |||||||||| |||||||||| .......... .......... .......... .......TCA TGCAATTCTT AGATATCTGG 274 CTTGTGCATT TCCAGGAATA GCAGATCATT GGTAAGTATC GACTTATTTT GAGTATATAC 5569 |||||||||| |||||||||| |||||||||| | CTTGTGCATT TCCAGGAATA GCAGATCATT G......... .......... .......... 305 TGTAGTCAAA TGCTTTGTAT CATGGAAATA AGTACTCTTT AAGCGCTTTT TCTAGACATA 5509 .......... .......... .......... .......... .......... .......... 305 GGATCCAACC CGGACAGATC AGGGATATAA ACTTGATGGG TTCAACTTTT ATATTCTCAC 5449 .......... .......... .......... .......... .......... .......... 305 TGCTGGTGCA ATCACGTGTA TACCTAAGTT CCATGTCAAA ATGTTGGGTT AAGCAGAACC 5389 .......... .......... .......... .......... .......... .......... 305 CATATTGCAT CTGCTGCTGA ACATGGCAAT CGGCATTACG AATGGAATTA TTCTGCAGTT 5329 .......... .......... .......... .......... .......... .......... 305 GGCATCTGTG TGAATTTAAT GCCACATGTA CCTGTTATTT GATTGATAAT TGTTTGTTGT 5269 .......... .......... .......... .......... .......... .......... 305 TTTTCTAACT TGGAGTTCAA CTAGTAGAGT TTTGATAAGA AACGCCATCG GGTTTTGCTA 5209 .......... .......... .......... .......... .......... .......... 305 AGAAACCCGT CATTTGTACC AAAACCGAGA ATAACATGAC ATGTGTCTTC TGCATACACT 5149 .......... .......... .......... .......... .......... .......... 305 TGTCACCTAA CTATTGTTAA TTAATGTGCA TTTTCATCTC ATTTATTACT TAAGAATGGT 5089 .......... .......... .......... .......... .......... .......... 305 AAAATAGTGA ACGAACCAAA GGAGAACTCC CAACAGAAAA GAAGAACAAG TAACATATTA 5029 .......... .......... .......... .......... .......... .......... 305 GCAGATTGGA AAATACCAGC TCAACAAATG AAGTATCAGC ACCAAATAAC AAACAAGCCC 4969 .......... .......... .......... .......... .......... .......... 305 TATCAAAATT TGAGAAGAGA TGACTATTGA ACACAGAAGT CATCCTTTGG AATCTCTGTA 4909 .......... .......... .......... .......... .......... .......... 305 CTATACCAAC TTATCCGAAA AAAGAAAAAA AAAGTACATC TATGGTGCCT ATGAACAATG 4849 .......... .......... .......... .......... .......... .......... 305 CTGCTCTTCA TTCTTAGTGA TAGACTAATG TACACACTCT TAGGTTGTAT AAATTCTTAT 4789 .......... .......... .......... .......... .......... .......... 305 CCTGGTTAGT GTTTGATTTA AATCCTCTTT CTTACACTTT CCTATTGAAG GTACCCTGCT 4729 |||||||||| .......... .......... .......... .......... .......... GTACCCTGCT 315 GACTTATACA AAAGAGCAAA GGTAGACTCT GTGTTGGACT GGCATCACTC AAACTTGCGT 4669 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACTTATACA AAAGAGCAAA GGTAGACTCT GTGTTGGACT GGCATCACTC AAACTTGCGT 375 CGCGGTGCAG GTGCTTATAT CAGATATTGA GATCATTTCA GTTTCCGGTT GAATTATGAT 4609 |||||||||| CGCGGTGCAG .......... .......... .......... .......... .......... 385 TACTTTACGT TGACCTTACT TGAGTTAATT TAGTGGTGAA TGCTGGGATA TGCAAGAACT 4549 .......... .......... .......... .......... .......... .......... 385 TGGGCAAAAA GATGCATACA GGCTACAGTT AAGGCAATGA CTGGATTAAA ATTTTATCCC 4489 .......... .......... .......... .......... .......... .......... 385 AAATAAGATT AATCAAGGAA ATCTGTTTGT ATGATCAGTT TTAAAAAAGA GTATCTGCTT 4429 .......... .......... .......... .......... .......... .......... 385 GTCATGATGC TCTTTGCTAA GATTCTCGCT ACTAACCTGA TACAAGAGAG TTGCTGCAAG 4369 .......... .......... .......... .......... .......... .......... 385 CCTACAAGTT TCTTAGTCTA TCTTTAGGAC TTGATTCCTC TAATTGAAAC ACCTGACCTT 4309 .......... .......... .......... .......... .......... .......... 385 TCTCAAACCT CTAATTGTGA GTCTAGCTAC TGCATGATGG TGACTTCTTT TCTTCAACAA 4249 .......... .......... .......... .......... .......... .......... 385 TAACAGTGAC TAAAACCACT AGGATAGGTA GTCTTTTCAC CAATGTTCTA TAATTACTAC 4189 .......... .......... .......... .......... .......... .......... 385 TTCTTGTTGA TAACTTATTA TGCCACTCTT TATCTGTTTC CAACAGTTTT CTGAAGCAGT 4129 .......... .......... .......... .......... .......... .......... 385 ACTTATGTTT TCAGCTGGAT ATATCTTTAA TACTGTTCTT GCTCCTGCTT TTGGGCTGCC 4069 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....CTGGAT ATATCTTTAA TACTGTTCTT GCTCCTGCTT TTGGGCTGCC 431 ATTGAATCCA CAAGCAGCAG CAG 4046 |||||||||| |||||||||| ||| ATTGAATCCA CAAGCAGCAG CAG 454 hqPGS_C08HBa0251H04.1-_SGN-E288767+ (7188 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4046) ******************************************************************************** EST sequence 128 +strand 443 n (File: SGN-E312633+) 1 GCTCGCGTTT CCGGCGGTGA GTTCGTCGGA GGTAATTTGG AGTGAGAATA GAATTGATTG 61 AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 121 ATCTTCTGCA AGTTAAATGG AATAGACTTT GAGGAGATAC ACATAAATCT CTCCAAGCGC 181 CAGCAGTTAT CTCCTGAATT TAAAGAAATT AATCCCATGA AGCAAGTACC CGCTATTATT 241 GATGGAAGAT TTAAGCTTTT TGAAAGTCAT GCAATTCTTA GATATCTGGC TTGTGCATTT 301 CCAGGAATAG CAGATCATTG GTACCCTGCT GACTTATACA AAAGAGCAAA GGTGGACTCT 361 GTGTTGGACT GGCATCACTC AAACTTGCGT CGCGGTGCAG CTGGATATAT CTTTAATACT 421 GTTCTTGCTC CTGCTTTTGG GCT Predicted gene structure (within gDNA segment 7893 to 3462): Exon 1 7203 7073 ( 131 n); cDNA 1 131 ( 131 n); score: 0.962 Intron 1 7072 6229 ( 844 n); Pd: 0.998 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 2 6228 6155 ( 74 n); cDNA 132 205 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 (s: 1.00), Pa: 0.960 (s: 0.96) Exon 3 6062 6002 ( 61 n); cDNA 206 266 ( 61 n); score: 0.951 Intron 3 6001 5652 ( 350 n); Pd: 0.991 (s: 0.94), Pa: 0.986 (s: 1.00) Exon 4 5651 5598 ( 54 n); cDNA 267 320 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 (s: 1.00), Pa: 0.999 (s: 0.98) Exon 5 4738 4659 ( 80 n); cDNA 321 400 ( 80 n); score: 0.988 Intron 5 4658 4115 ( 544 n); Pd: 0.850 (s: 0.98), Pa: 0.096 (s: 1.00) Exon 6 4114 4072 ( 43 n); cDNA 401 443 ( 43 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E312633+ 0.978 443 1.000 C PGS_C08HBa0251H04.1-_SGN-E312633+ (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4072) Alignment (genomic DNA sequence = upper lines): GCTCGCGTAG CCGGCGGTGA GTTCGTCGGA GTTAATTTCC AGTGAGAATA GAATTGATTG 7144 |||||||| |||||||||| |||||||||| | |||||| |||||||||| |||||||||| GCTCGCGTTT CCGGCGGTGA GTTCGTCGGA GGTAATTTGG AGTGAGAATA GAATTGATTG 60 AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 7084 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAGAGATGA CGTTGAAATT ATACGTAGAT CGTATGTCCC AACCTTCTCG TGCAGTTATC 120 ATCTTCTGCA AGTAAGTATT TTTCATTTTT CTTTTCTCGA GATGTTTAAA GCTCGTAATG 7024 |||||||||| | ATCTTCTGCA A......... .......... .......... .......... .......... 131 GAGGTTTTAA TGAATTATTT TCTAGAAATT TGATTAACTC CGTTAGATAG GAACAGATGA 6964 .......... .......... .......... .......... .......... .......... 131 TAAGTACTGA GTATTAGAAC GGAAAGATCT ATTAGATAAT GAGCTATTGA CACTATTCAT 6904 .......... .......... .......... .......... .......... .......... 131 CAACAATTCG TAGTGCTATG TTAATTCTAT ATGTAACTTT GAATCTCAAA ATGGAGTTTT 6844 .......... .......... .......... .......... .......... .......... 131 TAGTGACTGA TCTTTCAAAA AGTTTTTTTT GTTTTCTTTG AATCCTCATA TTTGTAGCTT 6784 .......... .......... .......... .......... .......... .......... 131 TAGAAGTTAC GAATACAATA ACATCAAGAA CATCCATAAC GTAGTTGTTA AAAGACTCTC 6724 .......... .......... .......... .......... .......... .......... 131 CGTTAGGCGA AATTTATTGA CATAAATATG CCCTTTAACT TGACTTTAAA TTACATTTAT 6664 .......... .......... .......... .......... .......... .......... 131 GTCCTTCAAC TTTGGGTGTG GACAAGTATA AATTTGTATA AAGTTGAATA AATAGACACG 6604 .......... .......... .......... .......... .......... .......... 131 TCATTTTTTG TCCTACGTTA TTGTGCCACG TAGGACTCAT GTGTGTACTT GTTCAAATTT 6544 .......... .......... .......... .......... .......... .......... 131 ATACAAGTTT GAGTATCTAC TTGTGCATAT CCATAATAGA AGGACATAAA TGTTAAATGA 6484 .......... .......... .......... .......... .......... .......... 131 GACCAAGTTA AATGACGCAT TTATGTATTA TGCTGCCAAG TTTAGGTGGG TAGGTTTATA 6424 .......... .......... .......... .......... .......... .......... 131 TGATTGTTTT TATATATAGG TAAATCAACC AAATCAAATT TTAAAAGAAT CTTTAGAGCT 6364 .......... .......... .......... .......... .......... .......... 131 TATTACAAGG GATTTGTTAA TGAGAATTTT TTAATCAAGA AGTTAATGAA GAAGTTCTGT 6304 .......... .......... .......... .......... .......... .......... 131 TGTTGTTTCT GGATAGTTCT GTTTCTAATT TTGAAGCTCA AAATGGATTT TTTAATGATT 6244 .......... .......... .......... .......... .......... .......... 131 TTAATTTTCC TAAAGGTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA 6184 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA 176 GCGCCAGCAG TTATCTCCTG AATTTAAAGG TTTGTCTTTT TTATTTACTC CACTTTTCGC 6124 |||||||||| |||||||||| ||||||||| GCGCCAGCAG TTATCTCCTG AATTTAAAG. .......... .......... .......... 205 TAGATATTAT TTATTTCTTA GTTTCAAGAT AATTTCAAAA GTTTTCTGAC TTCATCTTCA 6064 .......... .......... .......... .......... .......... .......... 205 GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG GAAGATTTAA GCTTTTCGAA 6004 ||||||||| |||||||||| |||||| ||| || ||||||| |||||||||| |||||| ||| .AAATTAATC CCATGAAGCA AGTACCCGCT ATTATTGATG GAAGATTTAA GCTTTTTGAA 264 AGGTAATATT CAGCTTGAAC TGAACATTTT AGCATTTTCC AGTATCACAA CTTTGTTAAC 5944 || AG........ .......... .......... .......... .......... .......... 266 GTCTATTATT CGCTGTATAT GTTGCATACA TCCTAAAGCT TATGATTTAG TCTTTCATTC 5884 .......... .......... .......... .......... .......... .......... 266 TCGTAGAAGT CATGTTTGTA GCGTTCACCT AATCACCTGT GCATATATAC TGCAAGAAGT 5824 .......... .......... .......... .......... .......... .......... 266 GAAAAAATTT GTAAAAATTT CTATGTTTTG TGTACACCAT GTGGGCACAA GTAACTACTG 5764 .......... .......... .......... .......... .......... .......... 266 TTGAAAATGC CTGTTGAGAC GGGACAATTT TATCTTTTTC TGAGTTTCAC ATAAAGTATT 5704 .......... .......... .......... .......... .......... .......... 266 TTATAACTTG GATAATGTTT AAAATTAACT AACTGATTAT ATGTCCTCGC AGTCATGCAA 5644 |||||||| .......... .......... .......... .......... .......... ..TCATGCAA 274 TTCTTAGATA TCTGGCTTGT GCATTTCCAG GAATAGCAGA TCATTGGTAA GTATCGACTT 5584 |||||||||| |||||||||| |||||||||| |||||||||| |||||| TTCTTAGATA TCTGGCTTGT GCATTTCCAG GAATAGCAGA TCATTG.... .......... 320 ATTTTGAGTA TATACTGTAG TCAAATGCTT TGTATCATGG AAATAAGTAC TCTTTAAGCG 5524 .......... .......... .......... .......... .......... .......... 320 CTTTTTCTAG ACATAGGATC CAACCCGGAC AGATCAGGGA TATAAACTTG ATGGGTTCAA 5464 .......... .......... .......... .......... .......... .......... 320 CTTTTATATT CTCACTGCTG GTGCAATCAC GTGTATACCT AAGTTCCATG TCAAAATGTT 5404 .......... .......... .......... .......... .......... .......... 320 GGGTTAAGCA GAACCCATAT TGCATCTGCT GCTGAACATG GCAATCGGCA TTACGAATGG 5344 .......... .......... .......... .......... .......... .......... 320 AATTATTCTG CAGTTGGCAT CTGTGTGAAT TTAATGCCAC ATGTACCTGT TATTTGATTG 5284 .......... .......... .......... .......... .......... .......... 320 ATAATTGTTT GTTGTTTTTC TAACTTGGAG TTCAACTAGT AGAGTTTTGA TAAGAAACGC 5224 .......... .......... .......... .......... .......... .......... 320 CATCGGGTTT TGCTAAGAAA CCCGTCATTT GTACCAAAAC CGAGAATAAC ATGACATGTG 5164 .......... .......... .......... .......... .......... .......... 320 TCTTCTGCAT ACACTTGTCA CCTAACTATT GTTAATTAAT GTGCATTTTC ATCTCATTTA 5104 .......... .......... .......... .......... .......... .......... 320 TTACTTAAGA ATGGTAAAAT AGTGAACGAA CCAAAGGAGA ACTCCCAACA GAAAAGAAGA 5044 .......... .......... .......... .......... .......... .......... 320 ACAAGTAACA TATTAGCAGA TTGGAAAATA CCAGCTCAAC AAATGAAGTA TCAGCACCAA 4984 .......... .......... .......... .......... .......... .......... 320 ATAACAAACA AGCCCTATCA AAATTTGAGA AGAGATGACT ATTGAACACA GAAGTCATCC 4924 .......... .......... .......... .......... .......... .......... 320 TTTGGAATCT CTGTACTATA CCAACTTATC CGAAAAAAGA AAAAAAAAGT ACATCTATGG 4864 .......... .......... .......... .......... .......... .......... 320 TGCCTATGAA CAATGCTGCT CTTCATTCTT AGTGATAGAC TAATGTACAC ACTCTTAGGT 4804 .......... .......... .......... .......... .......... .......... 320 TGTATAAATT CTTATCCTGG TTAGTGTTTG ATTTAAATCC TCTTTCTTAC ACTTTCCTAT 4744 .......... .......... .......... .......... .......... .......... 320 TGAAGGTACC CTGCTGACTT ATACAAAAGA GCAAAGGTAG ACTCTGTGTT GGACTGGCAT 4684 ||||| |||||||||| |||||||||| |||||||| | |||||||||| |||||||||| .....GTACC CTGCTGACTT ATACAAAAGA GCAAAGGTGG ACTCTGTGTT GGACTGGCAT 375 CACTCAAACT TGCGTCGCGG TGCAGGTGCT TATATCAGAT ATTGAGATCA TTTCAGTTTC 4624 |||||||||| |||||||||| ||||| CACTCAAACT TGCGTCGCGG TGCAG..... .......... .......... .......... 400 CGGTTGAATT ATGATTACTT TACGTTGACC TTACTTGAGT TAATTTAGTG GTGAATGCTG 4564 .......... .......... .......... .......... .......... .......... 400 GGATATGCAA GAACTTGGGC AAAAAGATGC ATACAGGCTA CAGTTAAGGC AATGACTGGA 4504 .......... .......... .......... .......... .......... .......... 400 TTAAAATTTT ATCCCAAATA AGATTAATCA AGGAAATCTG TTTGTATGAT CAGTTTTAAA 4444 .......... .......... .......... .......... .......... .......... 400 AAAGAGTATC TGCTTGTCAT GATGCTCTTT GCTAAGATTC TCGCTACTAA CCTGATACAA 4384 .......... .......... .......... .......... .......... .......... 400 GAGAGTTGCT GCAAGCCTAC AAGTTTCTTA GTCTATCTTT AGGACTTGAT TCCTCTAATT 4324 .......... .......... .......... .......... .......... .......... 400 GAAACACCTG ACCTTTCTCA AACCTCTAAT TGTGAGTCTA GCTACTGCAT GATGGTGACT 4264 .......... .......... .......... .......... .......... .......... 400 TCTTTTCTTC AACAATAACA GTGACTAAAA CCACTAGGAT AGGTAGTCTT TTCACCAATG 4204 .......... .......... .......... .......... .......... .......... 400 TTCTATAATT ACTACTTCTT GTTGATAACT TATTATGCCA CTCTTTATCT GTTTCCAACA 4144 .......... .......... .......... .......... .......... .......... 400 GTTTTCTGAA GCAGTACTTA TGTTTTCAGC TGGATATATC TTTAATACTG TTCTTGCTCC 4084 | |||||||||| |||||||||| |||||||||| .......... .......... .........C TGGATATATC TTTAATACTG TTCTTGCTCC 431 TGCTTTTGGG CT 4072 |||||||||| || TGCTTTTGGG CT 443 hqPGS_C08HBa0251H04.1-_SGN-E312633+ (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4072) ******************************************************************************** EST sequence 74 +strand 660 n (File: SGN-E210728+) 1 TTTGAGGAGA TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAGAA 61 ATTAATCCCA TGAAGCAAGT ACCAGCTATA ATTGATGGAA GATTTAAGCT TTTCGAAAGT 121 CATGCAATTC TTAGATATCT GGCTTGTGCA TTTCCAGGAA TAGCAGATCA TTGGTACCCT 181 GCTGACTTAT ACAAAAGAGC AAAGGTAGAC TCTGTGTTGG ACTGGCATCA CTCAAACTTG 241 CGTCGCGGTG CAGCTGGATA TATCTTTAAT ACTGTTCTTG CTCCTGCTTT TGGGCTGCCA 301 TTGAATCCAC AAGCAGCAGC AGAAGCTGAG AAAGTCCTTT TGGCATCTCT TGCAAAAGTC 361 GAGTCTGTTT GGCTCCAGAG AAAAGGACGG TTTTTGCTTG GGAGTGGCCA ACCTTCAATT 421 GCAGATCTTA GCTTAGTGTG TGAGATTATG GAACTTGAGA TTTTGGATGA GAAGGATCGT 481 GAGCGGATAA TAGGCCCATA CAAGAGAGTT TTGAAGTGGA TTGATGACAC AAAGAATGCC 541 ATGGAACCTC ATTTCCAAGA GGTACATGTG ATCCTCTTCA AAGCTAAAGA GAAGTTCCAC 601 AAGCAAAGAC ATGCTGTAGG AAGTAGCATT CCTCAATCGA GCAGAAAACC TGACTTGCAC Predicted gene structure (within gDNA segment 11757 to 6114): Exon 1 10910 10853 ( 58 n); cDNA 1 58 ( 58 n); score: 0.810 Intron 1 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 2 10740 10680 ( 61 n); cDNA 59 119 ( 61 n); score: 0.902 Intron 2 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 3 9985 9932 ( 54 n); cDNA 120 173 ( 54 n); score: 0.926 Intron 3 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 4 9750 9671 ( 80 n); cDNA 174 253 ( 80 n); score: 0.850 Intron 4 9670 9147 ( 524 n); Pd: 0.883 (s: 0.80), Pa: 0.000 (s: 0.78) Exon 5 9146 8941 ( 206 n); cDNA 254 459 ( 206 n); score: 0.850 Intron 5 8940 8563 ( 378 n); Pd: 0.997 (s: 0.96), Pa: 0.999 (s: 0.94) Exon 6 8562 8363 ( 200 n); cDNA 460 659 ( 200 n); score: 0.815 MATCH C08HBa0251H04.1- SGN-E210728+ 0.847 659 0.998 C PGS_C08HBa0251H04.1-_SGN-E210728+ (10910 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8363) Alignment (genomic DNA sequence = upper lines): TTTGAGGAAG TTCATATAGA TCTCTCCAAA CGCCAGCAAT TGTCTCCTGA ATATCGAGGT 10851 |||||||| | || ||| | ||||||||| |||||||| | | |||||||| || | || TTTGAGGAGA TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAG.. 58 TTGTGTTTTT TTAATAAACA ATACTTTTCC AACTAAGAAA GGGGTTGATT TGTTATTTGT 10791 .......... .......... .......... .......... .......... .......... 58 TTCTTAATTT TCCACATAAT TTCAATAGTT TCCTTTGTGT TCGTCTTCAG AAATTAATCC 10731 |||||||||| .......... .......... .......... .......... .......... AAATTAATCC 68 CATCCGGCAA ATACCAGCTA TAATGGATGG AAGATTTAAG CTTTCCGAAA GGTAATTTGA 10671 ||| |||| ||||||||| |||| ||||| |||||||||| |||| ||||| | CATGAAGCAA GTACCAGCTA TAATTGATGG AAGATTTAAG CTTTTCGAAA G......... 119 GCTTCAACTG AAAAGTTGGC ATTTTCCCAT ATTGCATTCA TCTTAAGCTT AATTCGAAAA 10611 .......... .......... .......... .......... .......... .......... 119 TACTGATACC GTTATATCTT GAAAGCGTTG TCAATGTCTT TATCATTTTA TGTGATCACT 10551 .......... .......... .......... .......... .......... .......... 119 TGTATATATG TATTATAAAG GCAAAATACT CAGTGGTAAG TGGAGAATAT TAATAAAGCT 10491 .......... .......... .......... .......... .......... .......... 119 TATATGTAGT GTGCTAGCGT AATGAAGCAC TAAACCTATG GGTAATGAAA CAGATTTGCC 10431 .......... .......... .......... .......... .......... .......... 119 CCATGGGGTA CAAGAAACTA CCATAGACAA TGCCCATGGA GACAAGTATG TCAAATAACT 10371 .......... .......... .......... .......... .......... .......... 119 TTTGGGCGAA GGGTTGAATT TGTCCTAAAA CTATGCGTAA TGAAACAGAT TTTCCCTCCG 10311 .......... .......... .......... .......... .......... .......... 119 TTAATAGTTG GGGTCAAATA TGCTCTGGCC GTTACTAAAA TGGGCTAAAA TTGCCCTTAT 10251 .......... .......... .......... .......... .......... .......... 119 TTTCAAATGC CAGAAGCTTA CCACCCGGTG ATGCCATGTA ATGGTGAGGG CCTAATTGAC 10191 .......... .......... .......... .......... .......... .......... 119 CTAACTATTA ACGGGAGGGC AAATATGTTC TATTTCACAC AGTTTTCGCA TAGTTCTAGG 10131 .......... .......... .......... .......... .......... .......... 119 TCAAATTTAA CCCTTTTCCC ATATTTTTCT CGCGGTGATA TGAGGCTGGG CCTTTTTCTT 10071 .......... .......... .......... .......... .......... .......... 119 TTGATCTTTT GCTGAGTTTC AGATAGAGTA ATGTATGACT TGGATAACAT GTAAATGTTC 10011 .......... .......... .......... .......... .......... .......... 119 GTTAACTAAC TTTATGTGTT TGCAGTCATG CAATTCTTAA ATACCTGGCT TGTGCATTTC 9951 ||||| ||||||||| ||| |||||| |||||||||| .......... .......... .....TCATG CAATTCTTAG ATATCTGGCT TGTGCATTTC 154 CACGAATTGC AGATCATTGG TAAGTTCAGC AAGTTTGAGT CTTTCAGTAT ATACCTGAAT 9891 || |||| || ||||||||| CAGGAATAGC AGATCATTG. .......... .......... .......... .......... 173 TGATGTTCTT TTTAATCATG GAAATGACAA TGAGTACACT TTAAATCTCT TGTGCTTGAC 9831 .......... .......... .......... .......... .......... .......... 173 AATGGGTACC CGACAATAGG CATTATGAAT CATCTTTTCT GCACTTAACA TCTCTTGTTT 9771 .......... .......... .......... .......... .......... .......... 173 CTTACACCGT CCTATTGAAG GTATCCAGCT GACTTATACA AAAGAGCAAA GGTTGAATCT 9711 ||| || ||| |||||||||| |||||||||| ||| || ||| .......... .......... GTACCCTGCT GACTTATACA AAAGAGCAAA GGTAGACTCT 213 GTGTTGGATT GGCATCGTAC TAACTTCCCT CGCGGTCCAG GTGCTTATAT CAGAGATTGT 9651 |||||||| | |||||| | ||||| | | |||||| ||| GTGTTGGACT GGCATCACTC AAACTTGCGT CGCGGTGCAG .......... .......... 253 GATTACTTTA GTTTCCAGTT GGAATTTGAT ATCTTGCTTG AGTTAATTTA ATGGTGAATC 9591 .......... .......... .......... .......... .......... .......... 253 TTGGGAGAAC TTGGACAACA AAATTGTTAA GGTTAAGTCT GCATCCGAGT TAAATTTTTA 9531 .......... .......... .......... .......... .......... .......... 253 TCCTAAAATG AGATTAACCA GGAAAATTTG TTTGTATGAT CTTTTCAAAA GAAGAGCAGC 9471 .......... .......... .......... .......... .......... .......... 253 AGGCATGCTA TAATGCTCTT TATTAAGATT CTGGTGACTA CCTAAATCAA GACTCTTTGC 9411 .......... .......... .......... .......... .......... .......... 253 TGCAAGTCTG CGAGTTTCTT ACTGTCTTTA GTCTTTAGTA CTTCTGTTTC TTGTAATTGA 9351 .......... .......... .......... .......... .......... .......... 253 AACAATTGAC CTTTGTCAAA ACTTTAGTTG CAAGTCTGGT CGATTATGAT GGTGATGTTT 9291 .......... .......... .......... .......... .......... .......... 253 TTTCCAGTTA TGATGATGAG TCGATGACTA ATAACACTTG AGATAAACGT GATTTATTCC 9231 .......... .......... .......... .......... .......... .......... 253 GCAGCATTCT GTGATTACTA CTTGTTGATA TGCCTCTCCG TTTAGCTATT TCCAACACTT 9171 .......... .......... .......... .......... .......... .......... 253 CCCTCAAGAA CGTTTCTGTT TCAGGTTCAT ATACCTTTTA TTCTGTTCTT GCTCCTACTG 9111 | || ||| |||| | | |||||||| |||||| || .......... .......... ....CTGGAT ATATCTTTAA TACTGTTCTT GCTCCTGCTT 289 TTGGGTTGCC TTTAAATACA AAAGCAGCAG CAAGAACTGA GAAGATGTTT ATAGCATGTC 9051 ||||| |||| || ||| || ||||||||| || | |||| ||| | || | |||| || TTGGGCTGCC ATTGAATCCA CAAGCAGCAG CAGAAGCTGA GAAAGTCCTT TTGGCATCTC 349 TTGCAACGAT CGAGTCTGTT TGGCTCCAGA AAAAAGGACG TTTTTTGCTT GGGAGTGACC 8991 |||||| | |||||||||| |||||||||| ||||||||| ||||||||| ||||||| || TTGCAAAAGT CGAGTCTGTT TGGCTCCAGA GAAAAGGACG GTTTTTGCTT GGGAGTGGCC 409 AACCTTCAAT TGCAGATCTT AGCTTAGCGT GTGAGATTAT GCAACTTGAG GTTAAGAGAC 8931 |||||||||| |||||||||| ||||||| || |||||||||| | |||||||| AACCTTCAAT TGCAGATCTT AGCTTAGTGT GTGAGATTAT GGAACTTGAG .......... 459 TTTCCCTTTT TTTCTTTTCT TTTTTGCTGT TGCTTTACTA TCTTTTGATT CAAAGCATAA 8871 .......... .......... .......... .......... .......... .......... 459 TTATCTATTG CTTCGGACAT TATTTGGAAC AAGGTTGGCT CAATTCAGAA CTGTATCAAA 8811 .......... .......... .......... .......... .......... .......... 459 ATGTTGCTTT TCTCTCCATC AGCTTGTATA AACTTCCACT GAAGTTGAAT AATAATACCC 8751 .......... .......... .......... .......... .......... .......... 459 ATAGTAATCA TCCTGTATAT GTGGTTTCAT TCAGCCGTCC TGTATATGTA GTTTCAGTCA 8691 .......... .......... .......... .......... .......... .......... 459 GCCGGTCATT TGCTTCTGTA TAGTCATTAC TAGTATAGCT GATGAAAGAA CGATAATTTT 8631 .......... .......... .......... .......... .......... .......... 459 CCGTCCATCA ACTGAATGCA GTTAATTCTG AGTTCTTATA GAAAACCATT TTTTCTTGCT 8571 .......... .......... .......... .......... .......... .......... 459 GAATTTAGAT TCTGGATGAG AAGGATCGTG AGCGGATATT AGGCCCATTC AAGAGAGTTC 8511 || | |||||||| |||||||||| |||||||| | |||||||| | ||||||||| ........AT TTTGGATGAG AAGGATCGTG AGCGGATAAT AGGCCCATAC AAGAGAGTTT 511 TGAAGTGGCT TGATGATACG AAGAATGCCA TGGCACCTCA TTTCGAGGAA GTACAATCAA 8451 |||||||| | |||||| || |||||||||| ||| |||||| |||| | || ||||| | TGAAGTGGAT TGATGACACA AAGAATGCCA TGGAACCTCA TTTCCAAGAG GTACATGTGA 571 CCCTCGCTGG TTATAAGGAG AAGGTGCAGA AGCAAAGAAA TACTTTAGGA AGTAAAATTA 8391 |||| ||| ||| ||| | || | |||||||| | | || ||||| |||| ||| TCCTCTTCAA AGCTAAAGAG AAGTTCCACA AGCAAAGACA TGCTGTAGGA AGTAGCATTC 631 CTCAATCGGG CAGAAAACCT GTCCTGCA 8363 |||||||| | |||||||||| | | |||| CTCAATCGAG CAGAAAACCT GACTTGCA 659 hqPGS_C08HBa0251H04.1-_SGN-E210728+ (10910 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8363) ******************************************************************************** EST sequence 78 +strand 737 n (File: SGN-E263709+) 1 AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 61 TCTGCAAGTT AAATGGAATA GACTTTGAGG AGATACACAT AAATCTCTCC AAGCGCCAGC 121 AGTTATCTCC TGAATTTAAA GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG 181 GAAGATTTAA GCTTTTCGAA AGTCATGCAA TTCTTAGATA TCTGGCTTGT GCATTTCCAG 241 GAATAGCAGA TCATTGGTAC CCTGCTGACT TATACAAAAG AGCAAAGGTA GACTCTGTGT 301 TGGACTGGCA TCACTCAAAC TTGCGTCGCG GTGCAGCTGG ATATATCTTT AATACTGTTC 361 TTGCTCCTGC TTTTGGGCTG CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC 421 TTTTGGCATC TCTTGCAAAA GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC 481 TTGGGAGTGG CCAACCTTCA ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG 541 AGATTTTGGA TGAGAAGGAT CGTGAGCGGA TAATAGGCCC ATACAAGAGA GTTTTGAAGT 601 GGATTGATGA CACAAAGAAT GCCATGGAAC CTCATTTCCA AGAGGTACAT GTGATCCTCT 661 TCAAGCTNAA GAGAAGTTCA CAAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCCTCATCG 721 AGCAGAAAAC CTGACTC Predicted gene structure (within gDNA segment 12587 to 6174): Exon 1 11442 11376 ( 67 n); cDNA 1 67 ( 67 n); score: 0.896 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 0.90), Pa: 0.992 (s: 0.86) Exon 2 10926 10853 ( 74 n); cDNA 68 141 ( 74 n); score: 0.838 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 3 10740 10680 ( 61 n); cDNA 142 202 ( 61 n); score: 0.902 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 4 9985 9932 ( 54 n); cDNA 203 256 ( 54 n); score: 0.926 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 5 9750 9671 ( 80 n); cDNA 257 336 ( 80 n); score: 0.850 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.80), Pa: 0.000 (s: 0.78) Exon 6 9146 8941 ( 206 n); cDNA 337 542 ( 206 n); score: 0.850 Intron 6 8940 8563 ( 378 n); Pd: 0.997 (s: 0.96), Pa: 0.999 (s: 0.94) Exon 7 8562 8370 ( 193 n); cDNA 543 733 ( 191 n); score: 0.803 MATCH C08HBa0251H04.1- SGN-E263709+ 0.850 735 0.997 C PGS_C08HBa0251H04.1-_SGN-E263709+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) Alignment (genomic DNA sequence = upper lines): AGATGACGCT CAAATTATAC GTAGATCGTA TGTCTCAAGC TTGTCGTGAA GTTATAATCT 11383 |||||||| | ||||||||| |||||||||| |||| ||| | || ||||| | ||||| |||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 60 TCTGCAAGTA AGCAATTTTT TGTTTGTTAG AATTAGTTTT GAAGCTCAAA TTGGAGCTTT 11323 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 67 ATACTTAAAT CTTTCAATTT AAAGTTTTTA GTATGAATAT GTTTTGTTGC AGAATAAGTT 11263 .......... .......... .......... .......... .......... .......... 67 GTTTTCTTAC TAATTAACTT GCATTTGAGG AGGAGAATTG CCAAGTTTTG CCCTATCTCA 11203 .......... .......... .......... .......... .......... .......... 67 TTAACCATTG ACGTAGGACT ATTGTCCTTT TGTCATTCTT TAACACCTAC CTCATAACTA 11143 .......... .......... .......... .......... .......... .......... 67 TGTTATCACG TAAAATTAGG TGTTAGGCCT AACTTACGTC CCAAAAGTTA GCTAAAAAAG 11083 .......... .......... .......... .......... .......... .......... 67 AGGAGTATTG CCCAAGCGTT ATAAGAAGTC TACCCATTTC ATTATCCATC GATGTGAGAC 11023 .......... .......... .......... .......... .......... .......... 67 TTTTGTCCTC CTTTAACAAT GGCGAGGAAG TTTTTCTTGA TATTGTTTGT AGTTTTGAAG 10963 .......... .......... .......... .......... .......... .......... 67 CTCAAAATGG AGTTATAATG ATTATTTTCC CAACAGGTTA AATGGGATAG ACTTTGAGGA 10903 |||| ||||| |||| |||||||||| .......... .......... .......... ......GTTA AATGGAATAG ACTTTGAGGA 91 AGTTCATATA GATCTCTCCA AACGCCAGCA ATTGTCTCCT GAATATCGAG GTTTGTGTTT 10843 | || ||| ||||||||| | |||||||| || |||||| |||| | || GATACACATA AATCTCTCCA AGCGCCAGCA GTTATCTCCT GAATTTAAAG .......... 141 TTTTAATAAA CAATACTTTT CCAACTAAGA AAGGGGTTGA TTTGTTATTT GTTTCTTAAT 10783 .......... .......... .......... .......... .......... .......... 141 TTTCCACATA ATTTCAATAG TTTCCTTTGT GTTCGTCTTC AGAAATTAAT CCCATCCGGC 10723 |||||||| ||||| || .......... .......... .......... .......... ..AAATTAAT CCCATGAAGC 159 AAATACCAGC TATAATGGAT GGAAGATTTA AGCTTTCCGA AAGGTAATTT GAGCTTCAAC 10663 || ||||||| |||||| ||| |||||||||| |||||| ||| ||| AAGTACCAGC TATAATTGAT GGAAGATTTA AGCTTTTCGA AAG....... .......... 202 TGAAAAGTTG GCATTTTCCC ATATTGCATT CATCTTAAGC TTAATTCGAA AATACTGATA 10603 .......... .......... .......... .......... .......... .......... 202 CCGTTATATC TTGAAAGCGT TGTCAATGTC TTTATCATTT TATGTGATCA CTTGTATATA 10543 .......... .......... .......... .......... .......... .......... 202 TGTATTATAA AGGCAAAATA CTCAGTGGTA AGTGGAGAAT ATTAATAAAG CTTATATGTA 10483 .......... .......... .......... .......... .......... .......... 202 GTGTGCTAGC GTAATGAAGC ACTAAACCTA TGGGTAATGA AACAGATTTG CCCCATGGGG 10423 .......... .......... .......... .......... .......... .......... 202 TACAAGAAAC TACCATAGAC AATGCCCATG GAGACAAGTA TGTCAAATAA CTTTTGGGCG 10363 .......... .......... .......... .......... .......... .......... 202 AAGGGTTGAA TTTGTCCTAA AACTATGCGT AATGAAACAG ATTTTCCCTC CGTTAATAGT 10303 .......... .......... .......... .......... .......... .......... 202 TGGGGTCAAA TATGCTCTGG CCGTTACTAA AATGGGCTAA AATTGCCCTT ATTTTCAAAT 10243 .......... .......... .......... .......... .......... .......... 202 GCCAGAAGCT TACCACCCGG TGATGCCATG TAATGGTGAG GGCCTAATTG ACCTAACTAT 10183 .......... .......... .......... .......... .......... .......... 202 TAACGGGAGG GCAAATATGT TCTATTTCAC ACAGTTTTCG CATAGTTCTA GGTCAAATTT 10123 .......... .......... .......... .......... .......... .......... 202 AACCCTTTTC CCATATTTTT CTCGCGGTGA TATGAGGCTG GGCCTTTTTC TTTTGATCTT 10063 .......... .......... .......... .......... .......... .......... 202 TTGCTGAGTT TCAGATAGAG TAATGTATGA CTTGGATAAC ATGTAAATGT TCGTTAACTA 10003 .......... .......... .......... .......... .......... .......... 202 ACTTTATGTG TTTGCAGTCA TGCAATTCTT AAATACCTGG CTTGTGCATT TCCACGAATT 9943 ||| |||||||||| | ||| |||| |||||||||| |||| |||| .......... .......TCA TGCAATTCTT AGATATCTGG CTTGTGCATT TCCAGGAATA 245 GCAGATCATT GGTAAGTTCA GCAAGTTTGA GTCTTTCAGT ATATACCTGA ATTGATGTTC 9883 |||||||||| | GCAGATCATT G......... .......... .......... .......... .......... 256 TTTTTAATCA TGGAAATGAC AATGAGTACA CTTTAAATCT CTTGTGCTTG ACAATGGGTA 9823 .......... .......... .......... .......... .......... .......... 256 CCCGACAATA GGCATTATGA ATCATCTTTT CTGCACTTAA CATCTCTTGT TTCTTACACC 9763 .......... .......... .......... .......... .......... .......... 256 GTCCTATTGA AGGTATCCAG CTGACTTATA CAAAAGAGCA AAGGTTGAAT CTGTGTTGGA 9703 ||| || | |||||||||| |||||||||| ||||| || | |||||||||| .......... ..GTACCCTG CTGACTTATA CAAAAGAGCA AAGGTAGACT CTGTGTTGGA 304 TTGGCATCGT ACTAACTTCC CTCGCGGTCC AGGTGCTTAT ATCAGAGATT GTGATTACTT 9643 ||||||| | ||||| | ||||||| | || CTGGCATCAC TCAAACTTGC GTCGCGGTGC AG........ .......... .......... 336 TAGTTTCCAG TTGGAATTTG ATATCTTGCT TGAGTTAATT TAATGGTGAA TCTTGGGAGA 9583 .......... .......... .......... .......... .......... .......... 336 ACTTGGACAA CAAAATTGTT AAGGTTAAGT CTGCATCCGA GTTAAATTTT TATCCTAAAA 9523 .......... .......... .......... .......... .......... .......... 336 TGAGATTAAC CAGGAAAATT TGTTTGTATG ATCTTTTCAA AAGAAGAGCA GCAGGCATGC 9463 .......... .......... .......... .......... .......... .......... 336 TATAATGCTC TTTATTAAGA TTCTGGTGAC TACCTAAATC AAGACTCTTT GCTGCAAGTC 9403 .......... .......... .......... .......... .......... .......... 336 TGCGAGTTTC TTACTGTCTT TAGTCTTTAG TACTTCTGTT TCTTGTAATT GAAACAATTG 9343 .......... .......... .......... .......... .......... .......... 336 ACCTTTGTCA AAACTTTAGT TGCAAGTCTG GTCGATTATG ATGGTGATGT TTTTTCCAGT 9283 .......... .......... .......... .......... .......... .......... 336 TATGATGATG AGTCGATGAC TAATAACACT TGAGATAAAC GTGATTTATT CCGCAGCATT 9223 .......... .......... .......... .......... .......... .......... 336 CTGTGATTAC TACTTGTTGA TATGCCTCTC CGTTTAGCTA TTTCCAACAC TTCCCTCAAG 9163 .......... .......... .......... .......... .......... .......... 336 AACGTTTCTG TTTCAGGTTC ATATACCTTT TATTCTGTTC TTGCTCCTAC TGTTGGGTTG 9103 | ||||| |||| || |||||| |||||||| | | ||||| || .......... ......CTGG ATATATCTTT AATACTGTTC TTGCTCCTGC TTTTGGGCTG 380 CCTTTAAATA CAAAAGCAGC AGCAAGAACT GAGAAGATGT TTATAGCATG TCTTGCAACG 9043 || || ||| || ||||||| |||| | || ||||| | || | |||| |||||||| CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC TTTTGGCATC TCTTGCAAAA 440 ATCGAGTCTG TTTGGCTCCA GAAAAAAGGA CGTTTTTTGC TTGGGAGTGA CCAACCTTCA 8983 ||||||||| |||||||||| || ||||||| || ||||||| ||||||||| |||||||||| GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC TTGGGAGTGG CCAACCTTCA 500 ATTGCAGATC TTAGCTTAGC GTGTGAGATT ATGCAACTTG AGGTTAAGAG ACTTTCCCTT 8923 |||||||||| ||||||||| |||||||||| ||| |||||| || ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG AG........ .......... 542 TTTTTCTTTT CTTTTTTGCT GTTGCTTTAC TATCTTTTGA TTCAAAGCAT AATTATCTAT 8863 .......... .......... .......... .......... .......... .......... 542 TGCTTCGGAC ATTATTTGGA ACAAGGTTGG CTCAATTCAG AACTGTATCA AAATGTTGCT 8803 .......... .......... .......... .......... .......... .......... 542 TTTCTCTCCA TCAGCTTGTA TAAACTTCCA CTGAAGTTGA ATAATAATAC CCATAGTAAT 8743 .......... .......... .......... .......... .......... .......... 542 CATCCTGTAT ATGTGGTTTC ATTCAGCCGT CCTGTATATG TAGTTTCAGT CAGCCGGTCA 8683 .......... .......... .......... .......... .......... .......... 542 TTTGCTTCTG TATAGTCATT ACTAGTATAG CTGATGAAAG AACGATAATT TTCCGTCCAT 8623 .......... .......... .......... .......... .......... .......... 542 CAACTGAATG CAGTTAATTC TGAGTTCTTA TAGAAAACCA TTTTTTCTTG CTGAATTTAG 8563 .......... .......... .......... .......... .......... .......... 542 ATTCTGGATG AGAAGGATCG TGAGCGGATA TTAGGCCCAT TCAAGAGAGT TCTGAAGTGG 8503 ||| |||||| |||||||||| |||||||||| ||||||||| ||||||||| | |||||||| ATTTTGGATG AGAAGGATCG TGAGCGGATA ATAGGCCCAT ACAAGAGAGT TTTGAAGTGG 602 CTTGATGATA CGAAGAATGC CATGGCACCT CATTTCGAGG AAGTACAATC AACCCTCGCT 8443 ||||||| | | |||||||| ||||| |||| |||||| | | | ||||| | |||| | ATTGATGACA CAAAGAATGC CATGGAACCT CATTTCCAAG AGGTACATGT GATCCTC-TT 661 GGTTATAAGG AGAAGGTGCA GAAGCAAAGA AATACTTTAG GAAGTAAAAT TACTCAATCG 8383 | | | |||| || || ||||||||| || || ||| |||||| || | | |||| CAAGCTNAAG AGAA-GTTCA CAAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCCTCATCG 720 GGCAGAAAAC CTG 8370 ||||||||| ||| AGCAGAAAAC CTG 733 hqPGS_C08HBa0251H04.1-_SGN-E263709+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) ******************************************************************************** EST sequence 137 +strand 737 n (File: SGN-E260745+) 1 AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 61 TCTGCAAGTT AAATGGAATA GACTTTGAGG AGATACACAT AAATCTCTCC AAGCGCCAGC 121 AGTTATCTCC TGAATTTAAA GAAATTAATC CCATGAAGCA AGTACCAGCT ATAATTGATG 181 GAAGATTTAA GCTTTTCGAA AGTCATGCAA TTCTTAGATA TCTGGCTTGT GCATTTCCAG 241 GAATAGCAGA TCATTGGTAC CCTGCTGACT TATACAAAAG AGCAAAGGTA GACTCTGTGT 301 TGGACTGGCA TCACTCAAAC TTGCGTCGCG GTGCAGCTGG ATATATCTTT AATACTGTTC 361 TTGCTCCTGC TTTTGGGCTG CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC 421 TTTTGGCATC TCTTGCAAAA GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC 481 TTGGGAGTGG CCAACCTTCA ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG 541 AGATTTTGGA TGAGAAGGAT CGTGAGCGGA TAATAGGCCC ATACAAGAAA GTTTTGAAGT 601 GGATTGATGA CACAAAGAAT GCCATGGAAC CTCATTTTCA AGAGGTACAT GTGATCCTCT 661 TCAAAGCTAA AGAGAAAGTC CACNAGCAAA GACATGCTGT AGGAAGTAGC ATTCCTCAAT 721 CGAGCAGAAA ACCTGAC Predicted gene structure (within gDNA segment 12587 to 6174): Exon 1 11442 11376 ( 67 n); cDNA 1 67 ( 67 n); score: 0.896 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 0.90), Pa: 0.992 (s: 0.86) Exon 2 10926 10853 ( 74 n); cDNA 68 141 ( 74 n); score: 0.838 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 3 10740 10680 ( 61 n); cDNA 142 202 ( 61 n); score: 0.902 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 4 9985 9932 ( 54 n); cDNA 203 256 ( 54 n); score: 0.926 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 5 9750 9671 ( 80 n); cDNA 257 336 ( 80 n); score: 0.850 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.80), Pa: 0.000 (s: 0.78) Exon 6 9146 8941 ( 206 n); cDNA 337 542 ( 206 n); score: 0.850 Intron 6 8940 8563 ( 378 n); Pd: 0.997 (s: 0.96), Pa: 0.999 (s: 0.92) Exon 7 8562 8370 ( 193 n); cDNA 543 735 ( 193 n); score: 0.803 MATCH C08HBa0251H04.1- SGN-E260745+ 0.850 735 0.997 C PGS_C08HBa0251H04.1-_SGN-E260745+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) Alignment (genomic DNA sequence = upper lines): AGATGACGCT CAAATTATAC GTAGATCGTA TGTCTCAAGC TTGTCGTGAA GTTATAATCT 11383 |||||||| | ||||||||| |||||||||| |||| ||| | || ||||| | ||||| |||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 60 TCTGCAAGTA AGCAATTTTT TGTTTGTTAG AATTAGTTTT GAAGCTCAAA TTGGAGCTTT 11323 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 67 ATACTTAAAT CTTTCAATTT AAAGTTTTTA GTATGAATAT GTTTTGTTGC AGAATAAGTT 11263 .......... .......... .......... .......... .......... .......... 67 GTTTTCTTAC TAATTAACTT GCATTTGAGG AGGAGAATTG CCAAGTTTTG CCCTATCTCA 11203 .......... .......... .......... .......... .......... .......... 67 TTAACCATTG ACGTAGGACT ATTGTCCTTT TGTCATTCTT TAACACCTAC CTCATAACTA 11143 .......... .......... .......... .......... .......... .......... 67 TGTTATCACG TAAAATTAGG TGTTAGGCCT AACTTACGTC CCAAAAGTTA GCTAAAAAAG 11083 .......... .......... .......... .......... .......... .......... 67 AGGAGTATTG CCCAAGCGTT ATAAGAAGTC TACCCATTTC ATTATCCATC GATGTGAGAC 11023 .......... .......... .......... .......... .......... .......... 67 TTTTGTCCTC CTTTAACAAT GGCGAGGAAG TTTTTCTTGA TATTGTTTGT AGTTTTGAAG 10963 .......... .......... .......... .......... .......... .......... 67 CTCAAAATGG AGTTATAATG ATTATTTTCC CAACAGGTTA AATGGGATAG ACTTTGAGGA 10903 |||| ||||| |||| |||||||||| .......... .......... .......... ......GTTA AATGGAATAG ACTTTGAGGA 91 AGTTCATATA GATCTCTCCA AACGCCAGCA ATTGTCTCCT GAATATCGAG GTTTGTGTTT 10843 | || ||| ||||||||| | |||||||| || |||||| |||| | || GATACACATA AATCTCTCCA AGCGCCAGCA GTTATCTCCT GAATTTAAAG .......... 141 TTTTAATAAA CAATACTTTT CCAACTAAGA AAGGGGTTGA TTTGTTATTT GTTTCTTAAT 10783 .......... .......... .......... .......... .......... .......... 141 TTTCCACATA ATTTCAATAG TTTCCTTTGT GTTCGTCTTC AGAAATTAAT CCCATCCGGC 10723 |||||||| ||||| || .......... .......... .......... .......... ..AAATTAAT CCCATGAAGC 159 AAATACCAGC TATAATGGAT GGAAGATTTA AGCTTTCCGA AAGGTAATTT GAGCTTCAAC 10663 || ||||||| |||||| ||| |||||||||| |||||| ||| ||| AAGTACCAGC TATAATTGAT GGAAGATTTA AGCTTTTCGA AAG....... .......... 202 TGAAAAGTTG GCATTTTCCC ATATTGCATT CATCTTAAGC TTAATTCGAA AATACTGATA 10603 .......... .......... .......... .......... .......... .......... 202 CCGTTATATC TTGAAAGCGT TGTCAATGTC TTTATCATTT TATGTGATCA CTTGTATATA 10543 .......... .......... .......... .......... .......... .......... 202 TGTATTATAA AGGCAAAATA CTCAGTGGTA AGTGGAGAAT ATTAATAAAG CTTATATGTA 10483 .......... .......... .......... .......... .......... .......... 202 GTGTGCTAGC GTAATGAAGC ACTAAACCTA TGGGTAATGA AACAGATTTG CCCCATGGGG 10423 .......... .......... .......... .......... .......... .......... 202 TACAAGAAAC TACCATAGAC AATGCCCATG GAGACAAGTA TGTCAAATAA CTTTTGGGCG 10363 .......... .......... .......... .......... .......... .......... 202 AAGGGTTGAA TTTGTCCTAA AACTATGCGT AATGAAACAG ATTTTCCCTC CGTTAATAGT 10303 .......... .......... .......... .......... .......... .......... 202 TGGGGTCAAA TATGCTCTGG CCGTTACTAA AATGGGCTAA AATTGCCCTT ATTTTCAAAT 10243 .......... .......... .......... .......... .......... .......... 202 GCCAGAAGCT TACCACCCGG TGATGCCATG TAATGGTGAG GGCCTAATTG ACCTAACTAT 10183 .......... .......... .......... .......... .......... .......... 202 TAACGGGAGG GCAAATATGT TCTATTTCAC ACAGTTTTCG CATAGTTCTA GGTCAAATTT 10123 .......... .......... .......... .......... .......... .......... 202 AACCCTTTTC CCATATTTTT CTCGCGGTGA TATGAGGCTG GGCCTTTTTC TTTTGATCTT 10063 .......... .......... .......... .......... .......... .......... 202 TTGCTGAGTT TCAGATAGAG TAATGTATGA CTTGGATAAC ATGTAAATGT TCGTTAACTA 10003 .......... .......... .......... .......... .......... .......... 202 ACTTTATGTG TTTGCAGTCA TGCAATTCTT AAATACCTGG CTTGTGCATT TCCACGAATT 9943 ||| |||||||||| | ||| |||| |||||||||| |||| |||| .......... .......TCA TGCAATTCTT AGATATCTGG CTTGTGCATT TCCAGGAATA 245 GCAGATCATT GGTAAGTTCA GCAAGTTTGA GTCTTTCAGT ATATACCTGA ATTGATGTTC 9883 |||||||||| | GCAGATCATT G......... .......... .......... .......... .......... 256 TTTTTAATCA TGGAAATGAC AATGAGTACA CTTTAAATCT CTTGTGCTTG ACAATGGGTA 9823 .......... .......... .......... .......... .......... .......... 256 CCCGACAATA GGCATTATGA ATCATCTTTT CTGCACTTAA CATCTCTTGT TTCTTACACC 9763 .......... .......... .......... .......... .......... .......... 256 GTCCTATTGA AGGTATCCAG CTGACTTATA CAAAAGAGCA AAGGTTGAAT CTGTGTTGGA 9703 ||| || | |||||||||| |||||||||| ||||| || | |||||||||| .......... ..GTACCCTG CTGACTTATA CAAAAGAGCA AAGGTAGACT CTGTGTTGGA 304 TTGGCATCGT ACTAACTTCC CTCGCGGTCC AGGTGCTTAT ATCAGAGATT GTGATTACTT 9643 ||||||| | ||||| | ||||||| | || CTGGCATCAC TCAAACTTGC GTCGCGGTGC AG........ .......... .......... 336 TAGTTTCCAG TTGGAATTTG ATATCTTGCT TGAGTTAATT TAATGGTGAA TCTTGGGAGA 9583 .......... .......... .......... .......... .......... .......... 336 ACTTGGACAA CAAAATTGTT AAGGTTAAGT CTGCATCCGA GTTAAATTTT TATCCTAAAA 9523 .......... .......... .......... .......... .......... .......... 336 TGAGATTAAC CAGGAAAATT TGTTTGTATG ATCTTTTCAA AAGAAGAGCA GCAGGCATGC 9463 .......... .......... .......... .......... .......... .......... 336 TATAATGCTC TTTATTAAGA TTCTGGTGAC TACCTAAATC AAGACTCTTT GCTGCAAGTC 9403 .......... .......... .......... .......... .......... .......... 336 TGCGAGTTTC TTACTGTCTT TAGTCTTTAG TACTTCTGTT TCTTGTAATT GAAACAATTG 9343 .......... .......... .......... .......... .......... .......... 336 ACCTTTGTCA AAACTTTAGT TGCAAGTCTG GTCGATTATG ATGGTGATGT TTTTTCCAGT 9283 .......... .......... .......... .......... .......... .......... 336 TATGATGATG AGTCGATGAC TAATAACACT TGAGATAAAC GTGATTTATT CCGCAGCATT 9223 .......... .......... .......... .......... .......... .......... 336 CTGTGATTAC TACTTGTTGA TATGCCTCTC CGTTTAGCTA TTTCCAACAC TTCCCTCAAG 9163 .......... .......... .......... .......... .......... .......... 336 AACGTTTCTG TTTCAGGTTC ATATACCTTT TATTCTGTTC TTGCTCCTAC TGTTGGGTTG 9103 | ||||| |||| || |||||| |||||||| | | ||||| || .......... ......CTGG ATATATCTTT AATACTGTTC TTGCTCCTGC TTTTGGGCTG 380 CCTTTAAATA CAAAAGCAGC AGCAAGAACT GAGAAGATGT TTATAGCATG TCTTGCAACG 9043 || || ||| || ||||||| |||| | || ||||| | || | |||| |||||||| CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC TTTTGGCATC TCTTGCAAAA 440 ATCGAGTCTG TTTGGCTCCA GAAAAAAGGA CGTTTTTTGC TTGGGAGTGA CCAACCTTCA 8983 ||||||||| |||||||||| || ||||||| || ||||||| ||||||||| |||||||||| GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC TTGGGAGTGG CCAACCTTCA 500 ATTGCAGATC TTAGCTTAGC GTGTGAGATT ATGCAACTTG AGGTTAAGAG ACTTTCCCTT 8923 |||||||||| ||||||||| |||||||||| ||| |||||| || ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG AG........ .......... 542 TTTTTCTTTT CTTTTTTGCT GTTGCTTTAC TATCTTTTGA TTCAAAGCAT AATTATCTAT 8863 .......... .......... .......... .......... .......... .......... 542 TGCTTCGGAC ATTATTTGGA ACAAGGTTGG CTCAATTCAG AACTGTATCA AAATGTTGCT 8803 .......... .......... .......... .......... .......... .......... 542 TTTCTCTCCA TCAGCTTGTA TAAACTTCCA CTGAAGTTGA ATAATAATAC CCATAGTAAT 8743 .......... .......... .......... .......... .......... .......... 542 CATCCTGTAT ATGTGGTTTC ATTCAGCCGT CCTGTATATG TAGTTTCAGT CAGCCGGTCA 8683 .......... .......... .......... .......... .......... .......... 542 TTTGCTTCTG TATAGTCATT ACTAGTATAG CTGATGAAAG AACGATAATT TTCCGTCCAT 8623 .......... .......... .......... .......... .......... .......... 542 CAACTGAATG CAGTTAATTC TGAGTTCTTA TAGAAAACCA TTTTTTCTTG CTGAATTTAG 8563 .......... .......... .......... .......... .......... .......... 542 ATTCTGGATG AGAAGGATCG TGAGCGGATA TTAGGCCCAT TCAAGAGAGT TCTGAAGTGG 8503 ||| |||||| |||||||||| |||||||||| ||||||||| ||||| ||| | |||||||| ATTTTGGATG AGAAGGATCG TGAGCGGATA ATAGGCCCAT ACAAGAAAGT TTTGAAGTGG 602 CTTGATGATA CGAAGAATGC CATGGCACCT CATTTCGAGG AAGTACAATC AACCCTCGCT 8443 ||||||| | | |||||||| ||||| |||| ||||| | | | ||||| | |||| ATTGATGACA CAAAGAATGC CATGGAACCT CATTTTCAAG AGGTACATGT GATCCTCTTC 662 GGTTATAAGG AGAAGGTGCA GAAGCAAAGA AATACTTTAG GAAGTAAAAT TACTCAATCG 8383 ||| | |||| || || |||||||| || || ||| |||||| || | |||||||| AAAGCTAAAG AGAAAGTCCA CNAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCTCAATCG 722 GGCAGAAAAC CTG 8370 ||||||||| ||| AGCAGAAAAC CTG 735 hqPGS_C08HBa0251H04.1-_SGN-E260745+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) ******************************************************************************** EST sequence 82 +strand 736 n (File: SGN-E261719+) 1 ATGACGTTGA AATTATACGT AGATCGTATG TCCCAACCTT CTCGTGCAGT TATCATCTTC 61 TGCAAGTTAA ATGGAATAGA CTTTGAGGAG ATACACATAA ATCTCTCCAA GCGCCAGCAG 121 TTATCTCCTG AATTTAAAGA AATTAATCCC ATGAAGCAAG TACCAGCTAT AATTGATGGA 181 AGATTTAAGC TTTTCGAAAG TCATGCAATT CTTAGATATC TGGCTTGTGC ATTTCCAGGA 241 ATAGCAGATC ATTGGTACCC TGCTGACTTA TACAAAAGAG CAAAGGTAGA CTCTGTGTTG 301 GACTGGCATC ACTCAAACTT GCGTCGCGGT GCAGCTGGAT ATATCTTTAA TACTGTTCTT 361 GCTCCTGCTT TTGGGCTGCC ATTGAATCCA CAAGCAGCAG CAGAAGCTGA GAAAGTCCTT 421 TTGGCATCTC TTGCAAAAGT CGAGTCTGTT TGGCTCCAGA GAAAAGGACG GTTTTTGCTT 481 GGGAGTGGCC AACCTTCAAT TGCAGATCTT AGCTTAGTGT GTGAGATTAT GGAACTTGAG 541 ATTTTGGATG AGAAGGATCG TGAGCGGATA ATAGGCCCAT ACAAGAGAGT TTTGAAGTGG 601 ATTGATGACA CAAAGAATGC CATGGAACCT CATTTCCAAG AGGTACATGT GATCCTCTTC 661 AAAGCTAAAG AGAAAGTTCA CAAGCAAAGA CATGCTGTAG GAAGTAGCAT TCCCTCATCG 721 AGCAGAAAAC CTGACT Predicted gene structure (within gDNA segment 12567 to 6164): Exon 1 11440 11376 ( 65 n); cDNA 1 65 ( 65 n); score: 0.892 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 0.90), Pa: 0.992 (s: 0.86) Exon 2 10926 10853 ( 74 n); cDNA 66 139 ( 74 n); score: 0.838 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 3 10740 10680 ( 61 n); cDNA 140 200 ( 61 n); score: 0.902 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 4 9985 9932 ( 54 n); cDNA 201 254 ( 54 n); score: 0.926 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 5 9750 9671 ( 80 n); cDNA 255 334 ( 80 n); score: 0.850 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.80), Pa: 0.000 (s: 0.78) Exon 6 9146 8941 ( 206 n); cDNA 335 540 ( 206 n); score: 0.850 Intron 6 8940 8563 ( 378 n); Pd: 0.997 (s: 0.96), Pa: 0.999 (s: 0.94) Exon 7 8562 8370 ( 193 n); cDNA 541 733 ( 193 n); score: 0.803 MATCH C08HBa0251H04.1- SGN-E261719+ 0.850 733 0.996 C PGS_C08HBa0251H04.1-_SGN-E261719+ (11440 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) Alignment (genomic DNA sequence = upper lines): ATGACGCTCA AATTATACGT AGATCGTATG TCTCAAGCTT GTCGTGAAGT TATAATCTTC 11381 |||||| | | |||||||||| |||||||||| || ||| ||| ||||| ||| ||| |||||| ATGACGTTGA AATTATACGT AGATCGTATG TCCCAACCTT CTCGTGCAGT TATCATCTTC 60 TGCAAGTAAG CAATTTTTTG TTTGTTAGAA TTAGTTTTGA AGCTCAAATT GGAGCTTTAT 11321 ||||| TGCAA..... .......... .......... .......... .......... .......... 65 ACTTAAATCT TTCAATTTAA AGTTTTTAGT ATGAATATGT TTTGTTGCAG AATAAGTTGT 11261 .......... .......... .......... .......... .......... .......... 65 TTTCTTACTA ATTAACTTGC ATTTGAGGAG GAGAATTGCC AAGTTTTGCC CTATCTCATT 11201 .......... .......... .......... .......... .......... .......... 65 AACCATTGAC GTAGGACTAT TGTCCTTTTG TCATTCTTTA ACACCTACCT CATAACTATG 11141 .......... .......... .......... .......... .......... .......... 65 TTATCACGTA AAATTAGGTG TTAGGCCTAA CTTACGTCCC AAAAGTTAGC TAAAAAAGAG 11081 .......... .......... .......... .......... .......... .......... 65 GAGTATTGCC CAAGCGTTAT AAGAAGTCTA CCCATTTCAT TATCCATCGA TGTGAGACTT 11021 .......... .......... .......... .......... .......... .......... 65 TTGTCCTCCT TTAACAATGG CGAGGAAGTT TTTCTTGATA TTGTTTGTAG TTTTGAAGCT 10961 .......... .......... .......... .......... .......... .......... 65 CAAAATGGAG TTATAATGAT TATTTTCCCA ACAGGTTAAA TGGGATAGAC TTTGAGGAAG 10901 |||||| ||| |||||| |||||||| .......... .......... .......... ....GTTAAA TGGAATAGAC TTTGAGGAGA 91 TTCATATAGA TCTCTCCAAA CGCCAGCAAT TGTCTCCTGA ATATCGAGGT TTGTGTTTTT 10841 | || ||| | ||||||||| |||||||| | | |||||||| || | || TACACATAAA TCTCTCCAAG CGCCAGCAGT TATCTCCTGA ATTTAAAG.. .......... 139 TTAATAAACA ATACTTTTCC AACTAAGAAA GGGGTTGATT TGTTATTTGT TTCTTAATTT 10781 .......... .......... .......... .......... .......... .......... 139 TCCACATAAT TTCAATAGTT TCCTTTGTGT TCGTCTTCAG AAATTAATCC CATCCGGCAA 10721 |||||||||| ||| |||| .......... .......... .......... .......... AAATTAATCC CATGAAGCAA 159 ATACCAGCTA TAATGGATGG AAGATTTAAG CTTTCCGAAA GGTAATTTGA GCTTCAACTG 10661 ||||||||| |||| ||||| |||||||||| |||| ||||| | GTACCAGCTA TAATTGATGG AAGATTTAAG CTTTTCGAAA G......... .......... 200 AAAAGTTGGC ATTTTCCCAT ATTGCATTCA TCTTAAGCTT AATTCGAAAA TACTGATACC 10601 .......... .......... .......... .......... .......... .......... 200 GTTATATCTT GAAAGCGTTG TCAATGTCTT TATCATTTTA TGTGATCACT TGTATATATG 10541 .......... .......... .......... .......... .......... .......... 200 TATTATAAAG GCAAAATACT CAGTGGTAAG TGGAGAATAT TAATAAAGCT TATATGTAGT 10481 .......... .......... .......... .......... .......... .......... 200 GTGCTAGCGT AATGAAGCAC TAAACCTATG GGTAATGAAA CAGATTTGCC CCATGGGGTA 10421 .......... .......... .......... .......... .......... .......... 200 CAAGAAACTA CCATAGACAA TGCCCATGGA GACAAGTATG TCAAATAACT TTTGGGCGAA 10361 .......... .......... .......... .......... .......... .......... 200 GGGTTGAATT TGTCCTAAAA CTATGCGTAA TGAAACAGAT TTTCCCTCCG TTAATAGTTG 10301 .......... .......... .......... .......... .......... .......... 200 GGGTCAAATA TGCTCTGGCC GTTACTAAAA TGGGCTAAAA TTGCCCTTAT TTTCAAATGC 10241 .......... .......... .......... .......... .......... .......... 200 CAGAAGCTTA CCACCCGGTG ATGCCATGTA ATGGTGAGGG CCTAATTGAC CTAACTATTA 10181 .......... .......... .......... .......... .......... .......... 200 ACGGGAGGGC AAATATGTTC TATTTCACAC AGTTTTCGCA TAGTTCTAGG TCAAATTTAA 10121 .......... .......... .......... .......... .......... .......... 200 CCCTTTTCCC ATATTTTTCT CGCGGTGATA TGAGGCTGGG CCTTTTTCTT TTGATCTTTT 10061 .......... .......... .......... .......... .......... .......... 200 GCTGAGTTTC AGATAGAGTA ATGTATGACT TGGATAACAT GTAAATGTTC GTTAACTAAC 10001 .......... .......... .......... .......... .......... .......... 200 TTTATGTGTT TGCAGTCATG CAATTCTTAA ATACCTGGCT TGTGCATTTC CACGAATTGC 9941 ||||| ||||||||| ||| |||||| |||||||||| || |||| || .......... .....TCATG CAATTCTTAG ATATCTGGCT TGTGCATTTC CAGGAATAGC 245 AGATCATTGG TAAGTTCAGC AAGTTTGAGT CTTTCAGTAT ATACCTGAAT TGATGTTCTT 9881 ||||||||| AGATCATTG. .......... .......... .......... .......... .......... 254 TTTAATCATG GAAATGACAA TGAGTACACT TTAAATCTCT TGTGCTTGAC AATGGGTACC 9821 .......... .......... .......... .......... .......... .......... 254 CGACAATAGG CATTATGAAT CATCTTTTCT GCACTTAACA TCTCTTGTTT CTTACACCGT 9761 .......... .......... .......... .......... .......... .......... 254 CCTATTGAAG GTATCCAGCT GACTTATACA AAAGAGCAAA GGTTGAATCT GTGTTGGATT 9701 ||| || ||| |||||||||| |||||||||| ||| || ||| |||||||| | .......... GTACCCTGCT GACTTATACA AAAGAGCAAA GGTAGACTCT GTGTTGGACT 304 GGCATCGTAC TAACTTCCCT CGCGGTCCAG GTGCTTATAT CAGAGATTGT GATTACTTTA 9641 |||||| | ||||| | | |||||| ||| GGCATCACTC AAACTTGCGT CGCGGTGCAG .......... .......... .......... 334 GTTTCCAGTT GGAATTTGAT ATCTTGCTTG AGTTAATTTA ATGGTGAATC TTGGGAGAAC 9581 .......... .......... .......... .......... .......... .......... 334 TTGGACAACA AAATTGTTAA GGTTAAGTCT GCATCCGAGT TAAATTTTTA TCCTAAAATG 9521 .......... .......... .......... .......... .......... .......... 334 AGATTAACCA GGAAAATTTG TTTGTATGAT CTTTTCAAAA GAAGAGCAGC AGGCATGCTA 9461 .......... .......... .......... .......... .......... .......... 334 TAATGCTCTT TATTAAGATT CTGGTGACTA CCTAAATCAA GACTCTTTGC TGCAAGTCTG 9401 .......... .......... .......... .......... .......... .......... 334 CGAGTTTCTT ACTGTCTTTA GTCTTTAGTA CTTCTGTTTC TTGTAATTGA AACAATTGAC 9341 .......... .......... .......... .......... .......... .......... 334 CTTTGTCAAA ACTTTAGTTG CAAGTCTGGT CGATTATGAT GGTGATGTTT TTTCCAGTTA 9281 .......... .......... .......... .......... .......... .......... 334 TGATGATGAG TCGATGACTA ATAACACTTG AGATAAACGT GATTTATTCC GCAGCATTCT 9221 .......... .......... .......... .......... .......... .......... 334 GTGATTACTA CTTGTTGATA TGCCTCTCCG TTTAGCTATT TCCAACACTT CCCTCAAGAA 9161 .......... .......... .......... .......... .......... .......... 334 CGTTTCTGTT TCAGGTTCAT ATACCTTTTA TTCTGTTCTT GCTCCTACTG TTGGGTTGCC 9101 | || ||| |||| | | |||||||| |||||| || ||||| |||| .......... ....CTGGAT ATATCTTTAA TACTGTTCTT GCTCCTGCTT TTGGGCTGCC 380 TTTAAATACA AAAGCAGCAG CAAGAACTGA GAAGATGTTT ATAGCATGTC TTGCAACGAT 9041 || ||| || ||||||||| || | |||| ||| | || | |||| || |||||| | ATTGAATCCA CAAGCAGCAG CAGAAGCTGA GAAAGTCCTT TTGGCATCTC TTGCAAAAGT 440 CGAGTCTGTT TGGCTCCAGA AAAAAGGACG TTTTTTGCTT GGGAGTGACC AACCTTCAAT 8981 |||||||||| |||||||||| ||||||||| ||||||||| ||||||| || |||||||||| CGAGTCTGTT TGGCTCCAGA GAAAAGGACG GTTTTTGCTT GGGAGTGGCC AACCTTCAAT 500 TGCAGATCTT AGCTTAGCGT GTGAGATTAT GCAACTTGAG GTTAAGAGAC TTTCCCTTTT 8921 |||||||||| ||||||| || |||||||||| | |||||||| TGCAGATCTT AGCTTAGTGT GTGAGATTAT GGAACTTGAG .......... .......... 540 TTTCTTTTCT TTTTTGCTGT TGCTTTACTA TCTTTTGATT CAAAGCATAA TTATCTATTG 8861 .......... .......... .......... .......... .......... .......... 540 CTTCGGACAT TATTTGGAAC AAGGTTGGCT CAATTCAGAA CTGTATCAAA ATGTTGCTTT 8801 .......... .......... .......... .......... .......... .......... 540 TCTCTCCATC AGCTTGTATA AACTTCCACT GAAGTTGAAT AATAATACCC ATAGTAATCA 8741 .......... .......... .......... .......... .......... .......... 540 TCCTGTATAT GTGGTTTCAT TCAGCCGTCC TGTATATGTA GTTTCAGTCA GCCGGTCATT 8681 .......... .......... .......... .......... .......... .......... 540 TGCTTCTGTA TAGTCATTAC TAGTATAGCT GATGAAAGAA CGATAATTTT CCGTCCATCA 8621 .......... .......... .......... .......... .......... .......... 540 ACTGAATGCA GTTAATTCTG AGTTCTTATA GAAAACCATT TTTTCTTGCT GAATTTAGAT 8561 || .......... .......... .......... .......... .......... ........AT 542 TCTGGATGAG AAGGATCGTG AGCGGATATT AGGCCCATTC AAGAGAGTTC TGAAGTGGCT 8501 | |||||||| |||||||||| |||||||| | |||||||| | ||||||||| |||||||| | TTTGGATGAG AAGGATCGTG AGCGGATAAT AGGCCCATAC AAGAGAGTTT TGAAGTGGAT 602 TGATGATACG AAGAATGCCA TGGCACCTCA TTTCGAGGAA GTACAATCAA CCCTCGCTGG 8441 |||||| || |||||||||| ||| |||||| |||| | || ||||| | |||| TGATGACACA AAGAATGCCA TGGAACCTCA TTTCCAAGAG GTACATGTGA TCCTCTTCAA 662 TTATAAGGAG AAGGTGCAGA AGCAAAGAAA TACTTTAGGA AGTAAAATTA CTCAATCGGG 8381 ||| ||| || || || | |||||||| | | || ||||| |||| ||| | |||| | AGCTAAAGAG AAAGTTCACA AGCAAAGACA TGCTGTAGGA AGTAGCATTC CCTCATCGAG 722 CAGAAAACCT G 8370 |||||||||| | CAGAAAACCT G 733 hqPGS_C08HBa0251H04.1-_SGN-E261719+ (11440 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) ******************************************************************************** EST sequence 87 +strand 688 n (File: SGN-E373791+) 1 GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 61 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 121 TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA GCAGTTATCT 181 CCTGAATTTA AAGAAATTAA TCCCATGAAG CAAGTACCAG CTATAATTGA TGGAAGATTT 241 AAGCTTTTCG AAAGTCATGC AATTCTTAGA TATCTGGCTT GTGCATTTCC AGGAATAGCA 301 GATCATTGGT ACCCTGCTGA CTTATACAAA AGAGCAAAGG TAGACTCTGT GTTGGACTGG 361 CATCACTCAA ACTTGCGTCG CGGTGCAGCT GGATATATCT TTAATACTGT TCTTGCTCCT 421 GCTTTTGGGC TGCCATTGAA TCCACAAGCA GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA 481 TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC CAGAGAAAAG GACGGTTTTT GCTTGGGAGT 541 GGCCAACCTT CAATTGCAGA TCTTAGCTTA GTGTGTGAGA TTATGGAACT TGAGATTTTG 601 GATGAGAAGG ATCGTGAGCG GATAATAGGC CCATACAAGA GAGTTTTGAA GTGGATTGAT 661 GACACANAGA ATGCCATGGA ACCTCATT Predicted gene structure (within gDNA segment 13107 to 7184): Exon 1 11442 11376 ( 67 n); cDNA 53 119 ( 67 n); score: 0.896 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 0.90), Pa: 0.992 (s: 0.86) Exon 2 10926 10853 ( 74 n); cDNA 120 193 ( 74 n); score: 0.838 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 3 10740 10680 ( 61 n); cDNA 194 254 ( 61 n); score: 0.902 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 4 9985 9932 ( 54 n); cDNA 255 308 ( 54 n); score: 0.926 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 5 9750 9671 ( 80 n); cDNA 309 388 ( 80 n); score: 0.850 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.80), Pa: 0.000 (s: 0.78) Exon 6 9146 8941 ( 206 n); cDNA 389 594 ( 206 n); score: 0.850 Intron 6 8940 8563 ( 378 n); Pd: 0.997 (s: 0.96), Pa: 0.999 (s: 0.94) Exon 7 8562 8469 ( 94 n); cDNA 595 688 ( 94 n); score: 0.904 MATCH C08HBa0251H04.1- SGN-E373791+ 0.873 636 0.924 C PGS_C08HBa0251H04.1-_SGN-E373791+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8469) Alignment (genomic DNA sequence = upper lines): AGATGACGCT CAAATTATAC GTAGATCGTA TGTCTCAAGC TTGTCGTGAA GTTATAATCT 11383 |||||||| | ||||||||| |||||||||| |||| ||| | || ||||| | ||||| |||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 112 TCTGCAAGTA AGCAATTTTT TGTTTGTTAG AATTAGTTTT GAAGCTCAAA TTGGAGCTTT 11323 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 119 ATACTTAAAT CTTTCAATTT AAAGTTTTTA GTATGAATAT GTTTTGTTGC AGAATAAGTT 11263 .......... .......... .......... .......... .......... .......... 119 GTTTTCTTAC TAATTAACTT GCATTTGAGG AGGAGAATTG CCAAGTTTTG CCCTATCTCA 11203 .......... .......... .......... .......... .......... .......... 119 TTAACCATTG ACGTAGGACT ATTGTCCTTT TGTCATTCTT TAACACCTAC CTCATAACTA 11143 .......... .......... .......... .......... .......... .......... 119 TGTTATCACG TAAAATTAGG TGTTAGGCCT AACTTACGTC CCAAAAGTTA GCTAAAAAAG 11083 .......... .......... .......... .......... .......... .......... 119 AGGAGTATTG CCCAAGCGTT ATAAGAAGTC TACCCATTTC ATTATCCATC GATGTGAGAC 11023 .......... .......... .......... .......... .......... .......... 119 TTTTGTCCTC CTTTAACAAT GGCGAGGAAG TTTTTCTTGA TATTGTTTGT AGTTTTGAAG 10963 .......... .......... .......... .......... .......... .......... 119 CTCAAAATGG AGTTATAATG ATTATTTTCC CAACAGGTTA AATGGGATAG ACTTTGAGGA 10903 |||| ||||| |||| |||||||||| .......... .......... .......... ......GTTA AATGGAATAG ACTTTGAGGA 143 AGTTCATATA GATCTCTCCA AACGCCAGCA ATTGTCTCCT GAATATCGAG GTTTGTGTTT 10843 | || ||| ||||||||| | |||||||| || |||||| |||| | || GATACACATA AATCTCTCCA AGCGCCAGCA GTTATCTCCT GAATTTAAAG .......... 193 TTTTAATAAA CAATACTTTT CCAACTAAGA AAGGGGTTGA TTTGTTATTT GTTTCTTAAT 10783 .......... .......... .......... .......... .......... .......... 193 TTTCCACATA ATTTCAATAG TTTCCTTTGT GTTCGTCTTC AGAAATTAAT CCCATCCGGC 10723 |||||||| ||||| || .......... .......... .......... .......... ..AAATTAAT CCCATGAAGC 211 AAATACCAGC TATAATGGAT GGAAGATTTA AGCTTTCCGA AAGGTAATTT GAGCTTCAAC 10663 || ||||||| |||||| ||| |||||||||| |||||| ||| ||| AAGTACCAGC TATAATTGAT GGAAGATTTA AGCTTTTCGA AAG....... .......... 254 TGAAAAGTTG GCATTTTCCC ATATTGCATT CATCTTAAGC TTAATTCGAA AATACTGATA 10603 .......... .......... .......... .......... .......... .......... 254 CCGTTATATC TTGAAAGCGT TGTCAATGTC TTTATCATTT TATGTGATCA CTTGTATATA 10543 .......... .......... .......... .......... .......... .......... 254 TGTATTATAA AGGCAAAATA CTCAGTGGTA AGTGGAGAAT ATTAATAAAG CTTATATGTA 10483 .......... .......... .......... .......... .......... .......... 254 GTGTGCTAGC GTAATGAAGC ACTAAACCTA TGGGTAATGA AACAGATTTG CCCCATGGGG 10423 .......... .......... .......... .......... .......... .......... 254 TACAAGAAAC TACCATAGAC AATGCCCATG GAGACAAGTA TGTCAAATAA CTTTTGGGCG 10363 .......... .......... .......... .......... .......... .......... 254 AAGGGTTGAA TTTGTCCTAA AACTATGCGT AATGAAACAG ATTTTCCCTC CGTTAATAGT 10303 .......... .......... .......... .......... .......... .......... 254 TGGGGTCAAA TATGCTCTGG CCGTTACTAA AATGGGCTAA AATTGCCCTT ATTTTCAAAT 10243 .......... .......... .......... .......... .......... .......... 254 GCCAGAAGCT TACCACCCGG TGATGCCATG TAATGGTGAG GGCCTAATTG ACCTAACTAT 10183 .......... .......... .......... .......... .......... .......... 254 TAACGGGAGG GCAAATATGT TCTATTTCAC ACAGTTTTCG CATAGTTCTA GGTCAAATTT 10123 .......... .......... .......... .......... .......... .......... 254 AACCCTTTTC CCATATTTTT CTCGCGGTGA TATGAGGCTG GGCCTTTTTC TTTTGATCTT 10063 .......... .......... .......... .......... .......... .......... 254 TTGCTGAGTT TCAGATAGAG TAATGTATGA CTTGGATAAC ATGTAAATGT TCGTTAACTA 10003 .......... .......... .......... .......... .......... .......... 254 ACTTTATGTG TTTGCAGTCA TGCAATTCTT AAATACCTGG CTTGTGCATT TCCACGAATT 9943 ||| |||||||||| | ||| |||| |||||||||| |||| |||| .......... .......TCA TGCAATTCTT AGATATCTGG CTTGTGCATT TCCAGGAATA 297 GCAGATCATT GGTAAGTTCA GCAAGTTTGA GTCTTTCAGT ATATACCTGA ATTGATGTTC 9883 |||||||||| | GCAGATCATT G......... .......... .......... .......... .......... 308 TTTTTAATCA TGGAAATGAC AATGAGTACA CTTTAAATCT CTTGTGCTTG ACAATGGGTA 9823 .......... .......... .......... .......... .......... .......... 308 CCCGACAATA GGCATTATGA ATCATCTTTT CTGCACTTAA CATCTCTTGT TTCTTACACC 9763 .......... .......... .......... .......... .......... .......... 308 GTCCTATTGA AGGTATCCAG CTGACTTATA CAAAAGAGCA AAGGTTGAAT CTGTGTTGGA 9703 ||| || | |||||||||| |||||||||| ||||| || | |||||||||| .......... ..GTACCCTG CTGACTTATA CAAAAGAGCA AAGGTAGACT CTGTGTTGGA 356 TTGGCATCGT ACTAACTTCC CTCGCGGTCC AGGTGCTTAT ATCAGAGATT GTGATTACTT 9643 ||||||| | ||||| | ||||||| | || CTGGCATCAC TCAAACTTGC GTCGCGGTGC AG........ .......... .......... 388 TAGTTTCCAG TTGGAATTTG ATATCTTGCT TGAGTTAATT TAATGGTGAA TCTTGGGAGA 9583 .......... .......... .......... .......... .......... .......... 388 ACTTGGACAA CAAAATTGTT AAGGTTAAGT CTGCATCCGA GTTAAATTTT TATCCTAAAA 9523 .......... .......... .......... .......... .......... .......... 388 TGAGATTAAC CAGGAAAATT TGTTTGTATG ATCTTTTCAA AAGAAGAGCA GCAGGCATGC 9463 .......... .......... .......... .......... .......... .......... 388 TATAATGCTC TTTATTAAGA TTCTGGTGAC TACCTAAATC AAGACTCTTT GCTGCAAGTC 9403 .......... .......... .......... .......... .......... .......... 388 TGCGAGTTTC TTACTGTCTT TAGTCTTTAG TACTTCTGTT TCTTGTAATT GAAACAATTG 9343 .......... .......... .......... .......... .......... .......... 388 ACCTTTGTCA AAACTTTAGT TGCAAGTCTG GTCGATTATG ATGGTGATGT TTTTTCCAGT 9283 .......... .......... .......... .......... .......... .......... 388 TATGATGATG AGTCGATGAC TAATAACACT TGAGATAAAC GTGATTTATT CCGCAGCATT 9223 .......... .......... .......... .......... .......... .......... 388 CTGTGATTAC TACTTGTTGA TATGCCTCTC CGTTTAGCTA TTTCCAACAC TTCCCTCAAG 9163 .......... .......... .......... .......... .......... .......... 388 AACGTTTCTG TTTCAGGTTC ATATACCTTT TATTCTGTTC TTGCTCCTAC TGTTGGGTTG 9103 | ||||| |||| || |||||| |||||||| | | ||||| || .......... ......CTGG ATATATCTTT AATACTGTTC TTGCTCCTGC TTTTGGGCTG 432 CCTTTAAATA CAAAAGCAGC AGCAAGAACT GAGAAGATGT TTATAGCATG TCTTGCAACG 9043 || || ||| || ||||||| |||| | || ||||| | || | |||| |||||||| CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC TTTTGGCATC TCTTGCAAAA 492 ATCGAGTCTG TTTGGCTCCA GAAAAAAGGA CGTTTTTTGC TTGGGAGTGA CCAACCTTCA 8983 ||||||||| |||||||||| || ||||||| || ||||||| ||||||||| |||||||||| GTCGAGTCTG TTTGGCTCCA GAGAAAAGGA CGGTTTTTGC TTGGGAGTGG CCAACCTTCA 552 ATTGCAGATC TTAGCTTAGC GTGTGAGATT ATGCAACTTG AGGTTAAGAG ACTTTCCCTT 8923 |||||||||| ||||||||| |||||||||| ||| |||||| || ATTGCAGATC TTAGCTTAGT GTGTGAGATT ATGGAACTTG AG........ .......... 594 TTTTTCTTTT CTTTTTTGCT GTTGCTTTAC TATCTTTTGA TTCAAAGCAT AATTATCTAT 8863 .......... .......... .......... .......... .......... .......... 594 TGCTTCGGAC ATTATTTGGA ACAAGGTTGG CTCAATTCAG AACTGTATCA AAATGTTGCT 8803 .......... .......... .......... .......... .......... .......... 594 TTTCTCTCCA TCAGCTTGTA TAAACTTCCA CTGAAGTTGA ATAATAATAC CCATAGTAAT 8743 .......... .......... .......... .......... .......... .......... 594 CATCCTGTAT ATGTGGTTTC ATTCAGCCGT CCTGTATATG TAGTTTCAGT CAGCCGGTCA 8683 .......... .......... .......... .......... .......... .......... 594 TTTGCTTCTG TATAGTCATT ACTAGTATAG CTGATGAAAG AACGATAATT TTCCGTCCAT 8623 .......... .......... .......... .......... .......... .......... 594 CAACTGAATG CAGTTAATTC TGAGTTCTTA TAGAAAACCA TTTTTTCTTG CTGAATTTAG 8563 .......... .......... .......... .......... .......... .......... 594 ATTCTGGATG AGAAGGATCG TGAGCGGATA TTAGGCCCAT TCAAGAGAGT TCTGAAGTGG 8503 ||| |||||| |||||||||| |||||||||| ||||||||| ||||||||| | |||||||| ATTTTGGATG AGAAGGATCG TGAGCGGATA ATAGGCCCAT ACAAGAGAGT TTTGAAGTGG 654 CTTGATGATA CGAAGAATGC CATGGCACCT CATT 8469 ||||||| | | ||||||| ||||| |||| |||| ATTGATGACA CANAGAATGC CATGGAACCT CATT 688 hqPGS_C08HBa0251H04.1-_SGN-E373791+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8469) ******************************************************************************** EST sequence 13 -strand 399 n (File: SGN-E223897-) 1 GCAAAGAAAT ACTTTAGGAA GTAAAATTAC TCAATCGGGC AGAAAACCTG TCCTGCAGTC 61 GAATATGTGA CACAGGAGTT AGGAAACCAG ACGCTGCATT ATGCTTGAAG CATCGCGGAT 121 GATTGTTTCT TCTACACTGT CTATTTGAAG CTGATCAGGA AGAGATTTCT AGTCCGAGCT 181 TTCCTACTAC TTTTTTCAAT TCTAAAAACG TTTTTCTTGA AATGAGAGTC TGTCATAAAC 241 AACCTCTCTA CCTCAAACAA GACGGGGTAA GATCCGCGTT CATTCTACTA CCTTCTCCAG 301 ACTTCACACT GGGTCTGTTG CTGTATTTGT ATTCATAAAA TAAGTGCACC GAATATGTAA 361 CAAGACAAGC TGAGTGTTCC TTAAAAAAAA AAAAAAAAA Predicted gene structure (within gDNA segment 9029 to 7286): Exon 1 8419 8036 ( 384 n); cDNA 1 384 ( 384 n); score: 1.000 PPA cDNA 385 399 MATCH C08HBa0251H04.1- SGN-E223897- 1.000 384 0.962 C PGS_C08HBa0251H04.1-_SGN-E223897- (8419 8036) Alignment (genomic DNA sequence = upper lines): GCAAAGAAAT ACTTTAGGAA GTAAAATTAC TCAATCGGGC AGAAAACCTG TCCTGCAGTC 8360 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCAAAGAAAT ACTTTAGGAA GTAAAATTAC TCAATCGGGC AGAAAACCTG TCCTGCAGTC 60 GAATATGTGA CACAGGAGTT AGGAAACCAG ACGCTGCATT ATGCTTGAAG CATCGCGGAT 8300 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAATATGTGA CACAGGAGTT AGGAAACCAG ACGCTGCATT ATGCTTGAAG CATCGCGGAT 120 GATTGTTTCT TCTACACTGT CTATTTGAAG CTGATCAGGA AGAGATTTCT AGTCCGAGCT 8240 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATTGTTTCT TCTACACTGT CTATTTGAAG CTGATCAGGA AGAGATTTCT AGTCCGAGCT 180 TTCCTACTAC TTTTTTCAAT TCTAAAAACG TTTTTCTTGA AATGAGAGTC TGTCATAAAC 8180 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCCTACTAC TTTTTTCAAT TCTAAAAACG TTTTTCTTGA AATGAGAGTC TGTCATAAAC 240 AACCTCTCTA CCTCAAACAA GACGGGGTAA GATCCGCGTT CATTCTACTA CCTTCTCCAG 8120 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACCTCTCTA CCTCAAACAA GACGGGGTAA GATCCGCGTT CATTCTACTA CCTTCTCCAG 300 ACTTCACACT GGGTCTGTTG CTGTATTTGT ATTCATAAAA TAAGTGCACC GAATATGTAA 8060 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTTCACACT GGGTCTGTTG CTGTATTTGT ATTCATAAAA TAAGTGCACC GAATATGTAA 360 CAAGACAAGC TGAGTGTTCC TTAA 8036 |||||||||| |||||||||| |||| CAAGACAAGC TGAGTGTTCC TTAA 384 hqPGS_C08HBa0251H04.1-_SGN-E223897- (8419 8036) ******************************************************************************** EST sequence 14 -strand 247 n (File: SGN-E224294-) 1 GATCAGAAAA AGATTTCTAG TCCGAGCTTT CCTACTACTT TTTTCAATTC TAAAAACGTT 61 TTTCTTGAAA TAAGAGTCTG TCATAAACAA CCTCTCTACC TCAAACAAGA CGGGGTAAGA 121 TCCGCGTTCA TTCTACTACC TTCTCCAGAC TTCACACTGG GTCTGTTGCT GTATTTGTAT 181 TCATAAAATA AGTGCACCGA ATATGTAACA AGACAAGCTG AGTGTTCCTT AAAAAAAAAA 241 AAAAAAA Predicted gene structure (within gDNA segment 8967 to 7286): Exon 1 8267 8036 ( 232 n); cDNA 1 232 ( 232 n); score: 0.987 PPA cDNA 233 247 MATCH C08HBa0251H04.1- SGN-E224294- 0.987 232 0.939 C PGS_C08HBa0251H04.1-_SGN-E224294- (8267 8036) Alignment (genomic DNA sequence = upper lines): GATCAGGAAG AGATTTCTAG TCCGAGCTTT CCTACTACTT TTTTCAATTC TAAAAACGTT 8208 |||||| || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATCAGAAAA AGATTTCTAG TCCGAGCTTT CCTACTACTT TTTTCAATTC TAAAAACGTT 60 TTTCTTGAAA TGAGAGTCTG TCATAAACAA CCTCTCTACC TCAAACAAGA CGGGGTAAGA 8148 |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCTTGAAA TAAGAGTCTG TCATAAACAA CCTCTCTACC TCAAACAAGA CGGGGTAAGA 120 TCCGCGTTCA TTCTACTACC TTCTCCAGAC TTCACACTGG GTCTGTTGCT GTATTTGTAT 8088 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCGCGTTCA TTCTACTACC TTCTCCAGAC TTCACACTGG GTCTGTTGCT GTATTTGTAT 180 TCATAAAATA AGTGCACCGA ATATGTAACA AGACAAGCTG AGTGTTCCTT AA 8036 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || TCATAAAATA AGTGCACCGA ATATGTAACA AGACAAGCTG AGTGTTCCTT AA 232 hqPGS_C08HBa0251H04.1-_SGN-E224294- (8267 8036) ******************************************************************************** EST sequence 80 +strand 598 n (File: SGN-E292336+) 1 GGCGGTGAGT TCGTCGGAGT TAATTTCCAG TGAGAATAGA ATTGATTGAA AGAGATGACG 61 TTGAAATTAT ACGTAGATCG TATGTCCCAA CCTTCTCGTG CAGTTATCAT CTTCTGCAAG 121 TTAAATGGAA TAGACTTTGA GGAGATACAC ATAAATCTCT CCAAGCGCCA GCAGTTATCT 181 CCTGAATTTA AAGAAATTAA TCCCATGAAG CAAGTACCAG CTATAATTGA TGGAAGATTT 241 AAGCTTTTCG AAAGTCATGC AATTCTTAGA TATCTGGCTT GTGCATTTCC AGGAATAGCA 301 GATCATTGGT ACCCTGCTGA CTTATACAAA AGAGCAAAGG TAGACTCTGT GTTGGACTGG 361 CATCACTCAA ACTTGCGTCG CGGTGCACCT GGATATATCT TTAATACTGA TCTTGCTCCT 421 GCTTTTGGGC TGCCATTGAA TCCACAAGCA GCAGCAGAAG CTGAGAAAGT CCTTTTGGCA 481 TCTCTTGCAA AAGTCGAGTC TGTTTGGCTC CAGAGAAAAC GACGGTTTTT GCTTGGGAGT 541 GGCCAACCTT CAATTGCAGA GCTTAGCTTA GTGTGTGAGA TTATGGAACT TGAGATTT Predicted gene structure (within gDNA segment 13107 to 7985): Exon 1 11442 11376 ( 67 n); cDNA 53 119 ( 67 n); score: 0.896 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 0.90), Pa: 0.992 (s: 0.86) Exon 2 10926 10853 ( 74 n); cDNA 120 193 ( 74 n); score: 0.838 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 0.78), Pa: 0.974 (s: 0.90) Exon 3 10740 10680 ( 61 n); cDNA 194 254 ( 61 n); score: 0.902 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 0.88), Pa: 0.997 (s: 0.92) Exon 4 9985 9932 ( 54 n); cDNA 255 308 ( 54 n); score: 0.926 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 0.92), Pa: 0.987 (s: 0.90) Exon 5 9750 9671 ( 80 n); cDNA 309 388 ( 80 n); score: 0.837 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.78), Pa: 0.000 (s: 0.76) Exon 6 9146 8941 ( 206 n); cDNA 389 594 ( 206 n); score: 0.835 MATCH C08HBa0251H04.1- SGN-E292336+ 0.860 542 0.906 C PGS_C08HBa0251H04.1-_SGN-E292336+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941) Alignment (genomic DNA sequence = upper lines): AGATGACGCT CAAATTATAC GTAGATCGTA TGTCTCAAGC TTGTCGTGAA GTTATAATCT 11383 |||||||| | ||||||||| |||||||||| |||| ||| | || ||||| | ||||| |||| AGATGACGTT GAAATTATAC GTAGATCGTA TGTCCCAACC TTCTCGTGCA GTTATCATCT 112 TCTGCAAGTA AGCAATTTTT TGTTTGTTAG AATTAGTTTT GAAGCTCAAA TTGGAGCTTT 11323 ||||||| TCTGCAA... .......... .......... .......... .......... .......... 119 ATACTTAAAT CTTTCAATTT AAAGTTTTTA GTATGAATAT GTTTTGTTGC AGAATAAGTT 11263 .......... .......... .......... .......... .......... .......... 119 GTTTTCTTAC TAATTAACTT GCATTTGAGG AGGAGAATTG CCAAGTTTTG CCCTATCTCA 11203 .......... .......... .......... .......... .......... .......... 119 TTAACCATTG ACGTAGGACT ATTGTCCTTT TGTCATTCTT TAACACCTAC CTCATAACTA 11143 .......... .......... .......... .......... .......... .......... 119 TGTTATCACG TAAAATTAGG TGTTAGGCCT AACTTACGTC CCAAAAGTTA GCTAAAAAAG 11083 .......... .......... .......... .......... .......... .......... 119 AGGAGTATTG CCCAAGCGTT ATAAGAAGTC TACCCATTTC ATTATCCATC GATGTGAGAC 11023 .......... .......... .......... .......... .......... .......... 119 TTTTGTCCTC CTTTAACAAT GGCGAGGAAG TTTTTCTTGA TATTGTTTGT AGTTTTGAAG 10963 .......... .......... .......... .......... .......... .......... 119 CTCAAAATGG AGTTATAATG ATTATTTTCC CAACAGGTTA AATGGGATAG ACTTTGAGGA 10903 |||| ||||| |||| |||||||||| .......... .......... .......... ......GTTA AATGGAATAG ACTTTGAGGA 143 AGTTCATATA GATCTCTCCA AACGCCAGCA ATTGTCTCCT GAATATCGAG GTTTGTGTTT 10843 | || ||| ||||||||| | |||||||| || |||||| |||| | || GATACACATA AATCTCTCCA AGCGCCAGCA GTTATCTCCT GAATTTAAAG .......... 193 TTTTAATAAA CAATACTTTT CCAACTAAGA AAGGGGTTGA TTTGTTATTT GTTTCTTAAT 10783 .......... .......... .......... .......... .......... .......... 193 TTTCCACATA ATTTCAATAG TTTCCTTTGT GTTCGTCTTC AGAAATTAAT CCCATCCGGC 10723 |||||||| ||||| || .......... .......... .......... .......... ..AAATTAAT CCCATGAAGC 211 AAATACCAGC TATAATGGAT GGAAGATTTA AGCTTTCCGA AAGGTAATTT GAGCTTCAAC 10663 || ||||||| |||||| ||| |||||||||| |||||| ||| ||| AAGTACCAGC TATAATTGAT GGAAGATTTA AGCTTTTCGA AAG....... .......... 254 TGAAAAGTTG GCATTTTCCC ATATTGCATT CATCTTAAGC TTAATTCGAA AATACTGATA 10603 .......... .......... .......... .......... .......... .......... 254 CCGTTATATC TTGAAAGCGT TGTCAATGTC TTTATCATTT TATGTGATCA CTTGTATATA 10543 .......... .......... .......... .......... .......... .......... 254 TGTATTATAA AGGCAAAATA CTCAGTGGTA AGTGGAGAAT ATTAATAAAG CTTATATGTA 10483 .......... .......... .......... .......... .......... .......... 254 GTGTGCTAGC GTAATGAAGC ACTAAACCTA TGGGTAATGA AACAGATTTG CCCCATGGGG 10423 .......... .......... .......... .......... .......... .......... 254 TACAAGAAAC TACCATAGAC AATGCCCATG GAGACAAGTA TGTCAAATAA CTTTTGGGCG 10363 .......... .......... .......... .......... .......... .......... 254 AAGGGTTGAA TTTGTCCTAA AACTATGCGT AATGAAACAG ATTTTCCCTC CGTTAATAGT 10303 .......... .......... .......... .......... .......... .......... 254 TGGGGTCAAA TATGCTCTGG CCGTTACTAA AATGGGCTAA AATTGCCCTT ATTTTCAAAT 10243 .......... .......... .......... .......... .......... .......... 254 GCCAGAAGCT TACCACCCGG TGATGCCATG TAATGGTGAG GGCCTAATTG ACCTAACTAT 10183 .......... .......... .......... .......... .......... .......... 254 TAACGGGAGG GCAAATATGT TCTATTTCAC ACAGTTTTCG CATAGTTCTA GGTCAAATTT 10123 .......... .......... .......... .......... .......... .......... 254 AACCCTTTTC CCATATTTTT CTCGCGGTGA TATGAGGCTG GGCCTTTTTC TTTTGATCTT 10063 .......... .......... .......... .......... .......... .......... 254 TTGCTGAGTT TCAGATAGAG TAATGTATGA CTTGGATAAC ATGTAAATGT TCGTTAACTA 10003 .......... .......... .......... .......... .......... .......... 254 ACTTTATGTG TTTGCAGTCA TGCAATTCTT AAATACCTGG CTTGTGCATT TCCACGAATT 9943 ||| |||||||||| | ||| |||| |||||||||| |||| |||| .......... .......TCA TGCAATTCTT AGATATCTGG CTTGTGCATT TCCAGGAATA 297 GCAGATCATT GGTAAGTTCA GCAAGTTTGA GTCTTTCAGT ATATACCTGA ATTGATGTTC 9883 |||||||||| | GCAGATCATT G......... .......... .......... .......... .......... 308 TTTTTAATCA TGGAAATGAC AATGAGTACA CTTTAAATCT CTTGTGCTTG ACAATGGGTA 9823 .......... .......... .......... .......... .......... .......... 308 CCCGACAATA GGCATTATGA ATCATCTTTT CTGCACTTAA CATCTCTTGT TTCTTACACC 9763 .......... .......... .......... .......... .......... .......... 308 GTCCTATTGA AGGTATCCAG CTGACTTATA CAAAAGAGCA AAGGTTGAAT CTGTGTTGGA 9703 ||| || | |||||||||| |||||||||| ||||| || | |||||||||| .......... ..GTACCCTG CTGACTTATA CAAAAGAGCA AAGGTAGACT CTGTGTTGGA 356 TTGGCATCGT ACTAACTTCC CTCGCGGTCC AGGTGCTTAT ATCAGAGATT GTGATTACTT 9643 ||||||| | ||||| | ||||||| | | CTGGCATCAC TCAAACTTGC GTCGCGGTGC AC........ .......... .......... 388 TAGTTTCCAG TTGGAATTTG ATATCTTGCT TGAGTTAATT TAATGGTGAA TCTTGGGAGA 9583 .......... .......... .......... .......... .......... .......... 388 ACTTGGACAA CAAAATTGTT AAGGTTAAGT CTGCATCCGA GTTAAATTTT TATCCTAAAA 9523 .......... .......... .......... .......... .......... .......... 388 TGAGATTAAC CAGGAAAATT TGTTTGTATG ATCTTTTCAA AAGAAGAGCA GCAGGCATGC 9463 .......... .......... .......... .......... .......... .......... 388 TATAATGCTC TTTATTAAGA TTCTGGTGAC TACCTAAATC AAGACTCTTT GCTGCAAGTC 9403 .......... .......... .......... .......... .......... .......... 388 TGCGAGTTTC TTACTGTCTT TAGTCTTTAG TACTTCTGTT TCTTGTAATT GAAACAATTG 9343 .......... .......... .......... .......... .......... .......... 388 ACCTTTGTCA AAACTTTAGT TGCAAGTCTG GTCGATTATG ATGGTGATGT TTTTTCCAGT 9283 .......... .......... .......... .......... .......... .......... 388 TATGATGATG AGTCGATGAC TAATAACACT TGAGATAAAC GTGATTTATT CCGCAGCATT 9223 .......... .......... .......... .......... .......... .......... 388 CTGTGATTAC TACTTGTTGA TATGCCTCTC CGTTTAGCTA TTTCCAACAC TTCCCTCAAG 9163 .......... .......... .......... .......... .......... .......... 388 AACGTTTCTG TTTCAGGTTC ATATACCTTT TATTCTGTTC TTGCTCCTAC TGTTGGGTTG 9103 | ||||| |||| || ||| || |||||||| | | ||||| || .......... ......CTGG ATATATCTTT AATACTGATC TTGCTCCTGC TTTTGGGCTG 432 CCTTTAAATA CAAAAGCAGC AGCAAGAACT GAGAAGATGT TTATAGCATG TCTTGCAACG 9043 || || ||| || ||||||| |||| | || ||||| | || | |||| |||||||| CCATTGAATC CACAAGCAGC AGCAGAAGCT GAGAAAGTCC TTTTGGCATC TCTTGCAAAA 492 ATCGAGTCTG TTTGGCTCCA GAAAAAAGGA CGTTTTTTGC TTGGGAGTGA CCAACCTTCA 8983 ||||||||| |||||||||| || |||| || || ||||||| ||||||||| |||||||||| GTCGAGTCTG TTTGGCTCCA GAGAAAACGA CGGTTTTTGC TTGGGAGTGG CCAACCTTCA 552 ATTGCAGATC TTAGCTTAGC GTGTGAGATT ATGCAACTTG AG 8941 |||||||| | ||||||||| |||||||||| ||| |||||| || ATTGCAGAGC TTAGCTTAGT GTGTGAGATT ATGGAACTTG AG 594 hqPGS_C08HBa0251H04.1-_SGN-E292336+ (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941) ******************************************************************************** EST sequence 107 +strand 496 n (File: SGN-E311539+) 1 ATAGAGATGA CGCTCAAATT ATACGTAGAT CGTATGTCTC AAGCTTGTCG TGAAGTTATA 61 ATCTTCTGCA AGTTAAATGG GATAGACTTT GAGGAAGTTC ATATAGATCT CTCCAAACGC 121 CAGCAATTGT CTCCTGAATA TCGAGAAATT AATCCCATCC GGCAAATACC AGCTATAATG 181 GATGGAAGAT TTAAGCTTTC CGAAAGTCAT GCAATTCTTA AATACCTGGC TTGTGCATTT 241 CCACGAATTG CAGATCATTG GTATCCAGCT GACTTATACA AAAGAGCAAA GGTTGAATCT 301 GTGTTGGATT GGCATCGTAC TAACTTCCCT CGCGGTCCAG GTTCATATAC CTTTTATTCT 361 GTTCTTGCTC CTACTGTTGG GTTGCCTTTA AATACAAAAG CAGCAGCAAG AACTGAGAAG 421 ATGTTTATAG CATGTCTTGC AACGATCGAG TCTGTTTGGC TCCAGAAAAA AGGACGTTTT 481 TTGCTTGGGA GTGACC Predicted gene structure (within gDNA segment 12046 to 8381): Exon 1 11446 11376 ( 71 n); cDNA 1 71 ( 71 n); score: 1.000 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 10926 10853 ( 74 n); cDNA 72 145 ( 74 n); score: 1.000 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 1.00), Pa: 0.974 (s: 1.00) Exon 3 10740 10680 ( 61 n); cDNA 146 206 ( 61 n); score: 1.000 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 4 9985 9932 ( 54 n); cDNA 207 260 ( 54 n); score: 1.000 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 5 9750 9671 ( 80 n); cDNA 261 340 ( 80 n); score: 1.000 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 6 9146 8991 ( 156 n); cDNA 341 496 ( 156 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E311539+ 1.000 496 1.000 C PGS_C08HBa0251H04.1-_SGN-E311539+ (11446 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8991) Alignment (genomic DNA sequence = upper lines): ATAGAGATGA CGCTCAAATT ATACGTAGAT CGTATGTCTC AAGCTTGTCG TGAAGTTATA 11387 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAGAGATGA CGCTCAAATT ATACGTAGAT CGTATGTCTC AAGCTTGTCG TGAAGTTATA 60 ATCTTCTGCA AGTAAGCAAT TTTTTGTTTG TTAGAATTAG TTTTGAAGCT CAAATTGGAG 11327 |||||||||| | ATCTTCTGCA A......... .......... .......... .......... .......... 71 CTTTATACTT AAATCTTTCA ATTTAAAGTT TTTAGTATGA ATATGTTTTG TTGCAGAATA 11267 .......... .......... .......... .......... .......... .......... 71 AGTTGTTTTC TTACTAATTA ACTTGCATTT GAGGAGGAGA ATTGCCAAGT TTTGCCCTAT 11207 .......... .......... .......... .......... .......... .......... 71 CTCATTAACC ATTGACGTAG GACTATTGTC CTTTTGTCAT TCTTTAACAC CTACCTCATA 11147 .......... .......... .......... .......... .......... .......... 71 ACTATGTTAT CACGTAAAAT TAGGTGTTAG GCCTAACTTA CGTCCCAAAA GTTAGCTAAA 11087 .......... .......... .......... .......... .......... .......... 71 AAAGAGGAGT ATTGCCCAAG CGTTATAAGA AGTCTACCCA TTTCATTATC CATCGATGTG 11027 .......... .......... .......... .......... .......... .......... 71 AGACTTTTGT CCTCCTTTAA CAATGGCGAG GAAGTTTTTC TTGATATTGT TTGTAGTTTT 10967 .......... .......... .......... .......... .......... .......... 71 GAAGCTCAAA ATGGAGTTAT AATGATTATT TTCCCAACAG GTTAAATGGG ATAGACTTTG 10907 |||||||||| |||||||||| .......... .......... .......... .......... GTTAAATGGG ATAGACTTTG 91 AGGAAGTTCA TATAGATCTC TCCAAACGCC AGCAATTGTC TCCTGAATAT CGAGGTTTGT 10847 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| AGGAAGTTCA TATAGATCTC TCCAAACGCC AGCAATTGTC TCCTGAATAT CGAG...... 145 GTTTTTTTAA TAAACAATAC TTTTCCAACT AAGAAAGGGG TTGATTTGTT ATTTGTTTCT 10787 .......... .......... .......... .......... .......... .......... 145 TAATTTTCCA CATAATTTCA ATAGTTTCCT TTGTGTTCGT CTTCAGAAAT TAATCCCATC 10727 |||| |||||||||| .......... .......... .......... .......... ......AAAT TAATCCCATC 159 CGGCAAATAC CAGCTATAAT GGATGGAAGA TTTAAGCTTT CCGAAAGGTA ATTTGAGCTT 10667 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| CGGCAAATAC CAGCTATAAT GGATGGAAGA TTTAAGCTTT CCGAAAG... .......... 206 CAACTGAAAA GTTGGCATTT TCCCATATTG CATTCATCTT AAGCTTAATT CGAAAATACT 10607 .......... .......... .......... .......... .......... .......... 206 GATACCGTTA TATCTTGAAA GCGTTGTCAA TGTCTTTATC ATTTTATGTG ATCACTTGTA 10547 .......... .......... .......... .......... .......... .......... 206 TATATGTATT ATAAAGGCAA AATACTCAGT GGTAAGTGGA GAATATTAAT AAAGCTTATA 10487 .......... .......... .......... .......... .......... .......... 206 TGTAGTGTGC TAGCGTAATG AAGCACTAAA CCTATGGGTA ATGAAACAGA TTTGCCCCAT 10427 .......... .......... .......... .......... .......... .......... 206 GGGGTACAAG AAACTACCAT AGACAATGCC CATGGAGACA AGTATGTCAA ATAACTTTTG 10367 .......... .......... .......... .......... .......... .......... 206 GGCGAAGGGT TGAATTTGTC CTAAAACTAT GCGTAATGAA ACAGATTTTC CCTCCGTTAA 10307 .......... .......... .......... .......... .......... .......... 206 TAGTTGGGGT CAAATATGCT CTGGCCGTTA CTAAAATGGG CTAAAATTGC CCTTATTTTC 10247 .......... .......... .......... .......... .......... .......... 206 AAATGCCAGA AGCTTACCAC CCGGTGATGC CATGTAATGG TGAGGGCCTA ATTGACCTAA 10187 .......... .......... .......... .......... .......... .......... 206 CTATTAACGG GAGGGCAAAT ATGTTCTATT TCACACAGTT TTCGCATAGT TCTAGGTCAA 10127 .......... .......... .......... .......... .......... .......... 206 ATTTAACCCT TTTCCCATAT TTTTCTCGCG GTGATATGAG GCTGGGCCTT TTTCTTTTGA 10067 .......... .......... .......... .......... .......... .......... 206 TCTTTTGCTG AGTTTCAGAT AGAGTAATGT ATGACTTGGA TAACATGTAA ATGTTCGTTA 10007 .......... .......... .......... .......... .......... .......... 206 ACTAACTTTA TGTGTTTGCA GTCATGCAAT TCTTAAATAC CTGGCTTGTG CATTTCCACG 9947 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .TCATGCAAT TCTTAAATAC CTGGCTTGTG CATTTCCACG 245 AATTGCAGAT CATTGGTAAG TTCAGCAAGT TTGAGTCTTT CAGTATATAC CTGAATTGAT 9887 |||||||||| ||||| AATTGCAGAT CATTG..... .......... .......... .......... .......... 260 GTTCTTTTTA ATCATGGAAA TGACAATGAG TACACTTTAA ATCTCTTGTG CTTGACAATG 9827 .......... .......... .......... .......... .......... .......... 260 GGTACCCGAC AATAGGCATT ATGAATCATC TTTTCTGCAC TTAACATCTC TTGTTTCTTA 9767 .......... .......... .......... .......... .......... .......... 260 CACCGTCCTA TTGAAGGTAT CCAGCTGACT TATACAAAAG AGCAAAGGTT GAATCTGTGT 9707 |||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ......GTAT CCAGCTGACT TATACAAAAG AGCAAAGGTT GAATCTGTGT 304 TGGATTGGCA TCGTACTAAC TTCCCTCGCG GTCCAGGTGC TTATATCAGA GATTGTGATT 9647 |||||||||| |||||||||| |||||||||| |||||| TGGATTGGCA TCGTACTAAC TTCCCTCGCG GTCCAG.... .......... .......... 340 ACTTTAGTTT CCAGTTGGAA TTTGATATCT TGCTTGAGTT AATTTAATGG TGAATCTTGG 9587 .......... .......... .......... .......... .......... .......... 340 GAGAACTTGG ACAACAAAAT TGTTAAGGTT AAGTCTGCAT CCGAGTTAAA TTTTTATCCT 9527 .......... .......... .......... .......... .......... .......... 340 AAAATGAGAT TAACCAGGAA AATTTGTTTG TATGATCTTT TCAAAAGAAG AGCAGCAGGC 9467 .......... .......... .......... .......... .......... .......... 340 ATGCTATAAT GCTCTTTATT AAGATTCTGG TGACTACCTA AATCAAGACT CTTTGCTGCA 9407 .......... .......... .......... .......... .......... .......... 340 AGTCTGCGAG TTTCTTACTG TCTTTAGTCT TTAGTACTTC TGTTTCTTGT AATTGAAACA 9347 .......... .......... .......... .......... .......... .......... 340 ATTGACCTTT GTCAAAACTT TAGTTGCAAG TCTGGTCGAT TATGATGGTG ATGTTTTTTC 9287 .......... .......... .......... .......... .......... .......... 340 CAGTTATGAT GATGAGTCGA TGACTAATAA CACTTGAGAT AAACGTGATT TATTCCGCAG 9227 .......... .......... .......... .......... .......... .......... 340 CATTCTGTGA TTACTACTTG TTGATATGCC TCTCCGTTTA GCTATTTCCA ACACTTCCCT 9167 .......... .......... .......... .......... .......... .......... 340 CAAGAACGTT TCTGTTTCAG GTTCATATAC CTTTTATTCT GTTCTTGCTC CTACTGTTGG 9107 |||||||||| |||||||||| |||||||||| |||||||||| .......... .......... GTTCATATAC CTTTTATTCT GTTCTTGCTC CTACTGTTGG 380 GTTGCCTTTA AATACAAAAG CAGCAGCAAG AACTGAGAAG ATGTTTATAG CATGTCTTGC 9047 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTGCCTTTA AATACAAAAG CAGCAGCAAG AACTGAGAAG ATGTTTATAG CATGTCTTGC 440 AACGATCGAG TCTGTTTGGC TCCAGAAAAA AGGACGTTTT TTGCTTGGGA GTGACC 8991 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| AACGATCGAG TCTGTTTGGC TCCAGAAAAA AGGACGTTTT TTGCTTGGGA GTGACC 496 hqPGS_C08HBa0251H04.1-_SGN-E311539+ (11446 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8991) ******************************************************************************** EST sequence 114 +strand 417 n (File: SGN-E289168+) 1 TCAGTTGACT GCGTCTAAAT CCAAAATAAG AAAATCGAAA GTTGAAGCAC AGTGGGAGAT 61 TATAGAGATG ACGCTCAAAT TATACGTAGA TCGTATGTCT CAAGCTTGTC GTGAAGTTAT 121 AATCTTCTGC AAGTTAAATG GGATAGACTT TGAGGAAGTT CATATAGATC TCTCCAAACG 181 CCAGCAATTG TCTCCTGAAT ATCGAGAAAT TAATCCCATC CGGCAAATAC CAGCTATAAT 241 GGATGGAAGA TTTAAGCTTT CCGAAAGTCA TGCAATTCTT AAATACCTGG CTTGTGCATT 301 TCCACGAATT GCAGATCATT GGTATCCAGC TGACTTATAC AAAAGAGCAA AAGTTGAATC 361 TGTGTTGGAT TGGCATCGTA CTAACTTCCC TCGCGGTCCA GGTTCATATA CCTTTTA Predicted gene structure (within gDNA segment 12215 to 8919): Exon 1 11507 11376 ( 132 n); cDNA 1 132 ( 132 n); score: 0.992 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 10926 10853 ( 74 n); cDNA 133 206 ( 74 n); score: 1.000 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 1.00), Pa: 0.974 (s: 1.00) Exon 3 10740 10680 ( 61 n); cDNA 207 267 ( 61 n); score: 1.000 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 4 9985 9932 ( 54 n); cDNA 268 321 ( 54 n); score: 1.000 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 1.00), Pa: 0.987 (s: 0.98) Exon 5 9750 9671 ( 80 n); cDNA 322 401 ( 80 n); score: 0.988 Intron 5 9670 9147 ( 524 n); Pd: 0.883 (s: 0.98), Pa: 0.000 (s: 0) Exon 6 9146 9131 ( 16 n); cDNA 402 417 ( 16 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E289168+ 0.995 417 1.000 C PGS_C08HBa0251H04.1-_SGN-E289168+ (11507 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 9131) Alignment (genomic DNA sequence = upper lines): TCAGTTGACT GTGTCTAAAT CCAAAATAAG AAAATCGAAA GTTGAAGCAC AGTGGGAGAT 11448 |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCAGTTGACT GCGTCTAAAT CCAAAATAAG AAAATCGAAA GTTGAAGCAC AGTGGGAGAT 60 TATAGAGATG ACGCTCAAAT TATACGTAGA TCGTATGTCT CAAGCTTGTC GTGAAGTTAT 11388 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATAGAGATG ACGCTCAAAT TATACGTAGA TCGTATGTCT CAAGCTTGTC GTGAAGTTAT 120 AATCTTCTGC AAGTAAGCAA TTTTTTGTTT GTTAGAATTA GTTTTGAAGC TCAAATTGGA 11328 |||||||||| || AATCTTCTGC AA........ .......... .......... .......... .......... 132 GCTTTATACT TAAATCTTTC AATTTAAAGT TTTTAGTATG AATATGTTTT GTTGCAGAAT 11268 .......... .......... .......... .......... .......... .......... 132 AAGTTGTTTT CTTACTAATT AACTTGCATT TGAGGAGGAG AATTGCCAAG TTTTGCCCTA 11208 .......... .......... .......... .......... .......... .......... 132 TCTCATTAAC CATTGACGTA GGACTATTGT CCTTTTGTCA TTCTTTAACA CCTACCTCAT 11148 .......... .......... .......... .......... .......... .......... 132 AACTATGTTA TCACGTAAAA TTAGGTGTTA GGCCTAACTT ACGTCCCAAA AGTTAGCTAA 11088 .......... .......... .......... .......... .......... .......... 132 AAAAGAGGAG TATTGCCCAA GCGTTATAAG AAGTCTACCC ATTTCATTAT CCATCGATGT 11028 .......... .......... .......... .......... .......... .......... 132 GAGACTTTTG TCCTCCTTTA ACAATGGCGA GGAAGTTTTT CTTGATATTG TTTGTAGTTT 10968 .......... .......... .......... .......... .......... .......... 132 TGAAGCTCAA AATGGAGTTA TAATGATTAT TTTCCCAACA GGTTAAATGG GATAGACTTT 10908 ||||||||| |||||||||| .......... .......... .......... .......... .GTTAAATGG GATAGACTTT 151 GAGGAAGTTC ATATAGATCT CTCCAAACGC CAGCAATTGT CTCCTGAATA TCGAGGTTTG 10848 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| GAGGAAGTTC ATATAGATCT CTCCAAACGC CAGCAATTGT CTCCTGAATA TCGAG..... 206 TGTTTTTTTA ATAAACAATA CTTTTCCAAC TAAGAAAGGG GTTGATTTGT TATTTGTTTC 10788 .......... .......... .......... .......... .......... .......... 206 TTAATTTTCC ACATAATTTC AATAGTTTCC TTTGTGTTCG TCTTCAGAAA TTAATCCCAT 10728 ||| |||||||||| .......... .......... .......... .......... .......AAA TTAATCCCAT 219 CCGGCAAATA CCAGCTATAA TGGATGGAAG ATTTAAGCTT TCCGAAAGGT AATTTGAGCT 10668 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| CCGGCAAATA CCAGCTATAA TGGATGGAAG ATTTAAGCTT TCCGAAAG.. .......... 267 TCAACTGAAA AGTTGGCATT TTCCCATATT GCATTCATCT TAAGCTTAAT TCGAAAATAC 10608 .......... .......... .......... .......... .......... .......... 267 TGATACCGTT ATATCTTGAA AGCGTTGTCA ATGTCTTTAT CATTTTATGT GATCACTTGT 10548 .......... .......... .......... .......... .......... .......... 267 ATATATGTAT TATAAAGGCA AAATACTCAG TGGTAAGTGG AGAATATTAA TAAAGCTTAT 10488 .......... .......... .......... .......... .......... .......... 267 ATGTAGTGTG CTAGCGTAAT GAAGCACTAA ACCTATGGGT AATGAAACAG ATTTGCCCCA 10428 .......... .......... .......... .......... .......... .......... 267 TGGGGTACAA GAAACTACCA TAGACAATGC CCATGGAGAC AAGTATGTCA AATAACTTTT 10368 .......... .......... .......... .......... .......... .......... 267 GGGCGAAGGG TTGAATTTGT CCTAAAACTA TGCGTAATGA AACAGATTTT CCCTCCGTTA 10308 .......... .......... .......... .......... .......... .......... 267 ATAGTTGGGG TCAAATATGC TCTGGCCGTT ACTAAAATGG GCTAAAATTG CCCTTATTTT 10248 .......... .......... .......... .......... .......... .......... 267 CAAATGCCAG AAGCTTACCA CCCGGTGATG CCATGTAATG GTGAGGGCCT AATTGACCTA 10188 .......... .......... .......... .......... .......... .......... 267 ACTATTAACG GGAGGGCAAA TATGTTCTAT TTCACACAGT TTTCGCATAG TTCTAGGTCA 10128 .......... .......... .......... .......... .......... .......... 267 AATTTAACCC TTTTCCCATA TTTTTCTCGC GGTGATATGA GGCTGGGCCT TTTTCTTTTG 10068 .......... .......... .......... .......... .......... .......... 267 ATCTTTTGCT GAGTTTCAGA TAGAGTAATG TATGACTTGG ATAACATGTA AATGTTCGTT 10008 .......... .......... .......... .......... .......... .......... 267 AACTAACTTT ATGTGTTTGC AGTCATGCAA TTCTTAAATA CCTGGCTTGT GCATTTCCAC 9948 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..TCATGCAA TTCTTAAATA CCTGGCTTGT GCATTTCCAC 305 GAATTGCAGA TCATTGGTAA GTTCAGCAAG TTTGAGTCTT TCAGTATATA CCTGAATTGA 9888 |||||||||| |||||| GAATTGCAGA TCATTG.... .......... .......... .......... .......... 321 TGTTCTTTTT AATCATGGAA ATGACAATGA GTACACTTTA AATCTCTTGT GCTTGACAAT 9828 .......... .......... .......... .......... .......... .......... 321 GGGTACCCGA CAATAGGCAT TATGAATCAT CTTTTCTGCA CTTAACATCT CTTGTTTCTT 9768 .......... .......... .......... .......... .......... .......... 321 ACACCGTCCT ATTGAAGGTA TCCAGCTGAC TTATACAAAA GAGCAAAGGT TGAATCTGTG 9708 ||| |||||||||| |||||||||| ||||||| || |||||||||| .......... .......GTA TCCAGCTGAC TTATACAAAA GAGCAAAAGT TGAATCTGTG 364 TTGGATTGGC ATCGTACTAA CTTCCCTCGC GGTCCAGGTG CTTATATCAG AGATTGTGAT 9648 |||||||||| |||||||||| |||||||||| ||||||| TTGGATTGGC ATCGTACTAA CTTCCCTCGC GGTCCAG... .......... .......... 401 TACTTTAGTT TCCAGTTGGA ATTTGATATC TTGCTTGAGT TAATTTAATG GTGAATCTTG 9588 .......... .......... .......... .......... .......... .......... 401 GGAGAACTTG GACAACAAAA TTGTTAAGGT TAAGTCTGCA TCCGAGTTAA ATTTTTATCC 9528 .......... .......... .......... .......... .......... .......... 401 TAAAATGAGA TTAACCAGGA AAATTTGTTT GTATGATCTT TTCAAAAGAA GAGCAGCAGG 9468 .......... .......... .......... .......... .......... .......... 401 CATGCTATAA TGCTCTTTAT TAAGATTCTG GTGACTACCT AAATCAAGAC TCTTTGCTGC 9408 .......... .......... .......... .......... .......... .......... 401 AAGTCTGCGA GTTTCTTACT GTCTTTAGTC TTTAGTACTT CTGTTTCTTG TAATTGAAAC 9348 .......... .......... .......... .......... .......... .......... 401 AATTGACCTT TGTCAAAACT TTAGTTGCAA GTCTGGTCGA TTATGATGGT GATGTTTTTT 9288 .......... .......... .......... .......... .......... .......... 401 CCAGTTATGA TGATGAGTCG ATGACTAATA ACACTTGAGA TAAACGTGAT TTATTCCGCA 9228 .......... .......... .......... .......... .......... .......... 401 GCATTCTGTG ATTACTACTT GTTGATATGC CTCTCCGTTT AGCTATTTCC AACACTTCCC 9168 .......... .......... .......... .......... .......... .......... 401 TCAAGAACGT TTCTGTTTCA GGTTCATATA CCTTTTA 9131 ||||||||| ||||||| .......... .......... .GTTCATATA CCTTTTA 417 hqPGS_C08HBa0251H04.1-_SGN-E289168+ (11507 11376,10926 10853,10740 10680,9985 9932,9750 9671) ******************************************************************************** EST sequence 133 +strand 314 n (File: SGN-E245867+) 1 GAAAATCGAA AGTTGAAGCA CAGTGGGAGA TTATAGAGAT GACGCTCAAA TTATACGTAG 61 ATCGTATGTC TCAAGCTTGT CGTGAAGTTA TAATCTTCTG CAAGTTAAAT GGGATAGACT 121 TTGAGGAAGT TCATATAGAT CTCTCCAAAC GCCAGCAATT GTCTCCTGAA TATCGAGAAA 181 TTAATCCCAT CCGGCAAATA CCAGCTATAA TGGATGGAAG ATTTAAGCTT TCCGAAAGTC 241 ATGCAATTCT TAAATACCTG GCTTGTGCAT TTCCACGAAT TGCAGATCAT TGGTATCCAG 301 CTGACTTATA CAAA Predicted gene structure (within gDNA segment 12078 to 9129): Exon 1 11478 11376 ( 103 n); cDNA 1 103 ( 103 n); score: 1.000 Intron 1 11375 10927 ( 449 n); Pd: 0.595 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 10926 10853 ( 74 n); cDNA 104 177 ( 74 n); score: 1.000 Intron 2 10852 10741 ( 112 n); Pd: 0.985 (s: 1.00), Pa: 0.974 (s: 1.00) Exon 3 10740 10680 ( 61 n); cDNA 178 238 ( 61 n); score: 1.000 Intron 3 10679 9986 ( 694 n); Pd: 0.987 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 4 9985 9932 ( 54 n); cDNA 239 292 ( 54 n); score: 1.000 Intron 4 9931 9751 ( 181 n); Pd: 0.995 (s: 1.00), Pa: 0.987 (s: 0) Exon 5 9750 9729 ( 22 n); cDNA 293 314 ( 22 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E245867+ 1.000 314 1.000 C PGS_C08HBa0251H04.1-_SGN-E245867+ (11478 11376,10926 10853,10740 10680,9985 9932,9750 9729) Alignment (genomic DNA sequence = upper lines): GAAAATCGAA AGTTGAAGCA CAGTGGGAGA TTATAGAGAT GACGCTCAAA TTATACGTAG 11419 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAAATCGAA AGTTGAAGCA CAGTGGGAGA TTATAGAGAT GACGCTCAAA TTATACGTAG 60 ATCGTATGTC TCAAGCTTGT CGTGAAGTTA TAATCTTCTG CAAGTAAGCA ATTTTTTGTT 11359 |||||||||| |||||||||| |||||||||| |||||||||| ||| ATCGTATGTC TCAAGCTTGT CGTGAAGTTA TAATCTTCTG CAA....... .......... 103 TGTTAGAATT AGTTTTGAAG CTCAAATTGG AGCTTTATAC TTAAATCTTT CAATTTAAAG 11299 .......... .......... .......... .......... .......... .......... 103 TTTTTAGTAT GAATATGTTT TGTTGCAGAA TAAGTTGTTT TCTTACTAAT TAACTTGCAT 11239 .......... .......... .......... .......... .......... .......... 103 TTGAGGAGGA GAATTGCCAA GTTTTGCCCT ATCTCATTAA CCATTGACGT AGGACTATTG 11179 .......... .......... .......... .......... .......... .......... 103 TCCTTTTGTC ATTCTTTAAC ACCTACCTCA TAACTATGTT ATCACGTAAA ATTAGGTGTT 11119 .......... .......... .......... .......... .......... .......... 103 AGGCCTAACT TACGTCCCAA AAGTTAGCTA AAAAAGAGGA GTATTGCCCA AGCGTTATAA 11059 .......... .......... .......... .......... .......... .......... 103 GAAGTCTACC CATTTCATTA TCCATCGATG TGAGACTTTT GTCCTCCTTT AACAATGGCG 10999 .......... .......... .......... .......... .......... .......... 103 AGGAAGTTTT TCTTGATATT GTTTGTAGTT TTGAAGCTCA AAATGGAGTT ATAATGATTA 10939 .......... .......... .......... .......... .......... .......... 103 TTTTCCCAAC AGGTTAAATG GGATAGACTT TGAGGAAGTT CATATAGATC TCTCCAAACG 10879 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..GTTAAATG GGATAGACTT TGAGGAAGTT CATATAGATC TCTCCAAACG 151 CCAGCAATTG TCTCCTGAAT ATCGAGGTTT GTGTTTTTTT AATAAACAAT ACTTTTCCAA 10819 |||||||||| |||||||||| |||||| CCAGCAATTG TCTCCTGAAT ATCGAG.... .......... .......... .......... 177 CTAAGAAAGG GGTTGATTTG TTATTTGTTT CTTAATTTTC CACATAATTT CAATAGTTTC 10759 .......... .......... .......... .......... .......... .......... 177 CTTTGTGTTC GTCTTCAGAA ATTAATCCCA TCCGGCAAAT ACCAGCTATA ATGGATGGAA 10699 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........AA ATTAATCCCA TCCGGCAAAT ACCAGCTATA ATGGATGGAA 219 GATTTAAGCT TTCCGAAAGG TAATTTGAGC TTCAACTGAA AAGTTGGCAT TTTCCCATAT 10639 |||||||||| ||||||||| GATTTAAGCT TTCCGAAAG. .......... .......... .......... .......... 238 TGCATTCATC TTAAGCTTAA TTCGAAAATA CTGATACCGT TATATCTTGA AAGCGTTGTC 10579 .......... .......... .......... .......... .......... .......... 238 AATGTCTTTA TCATTTTATG TGATCACTTG TATATATGTA TTATAAAGGC AAAATACTCA 10519 .......... .......... .......... .......... .......... .......... 238 GTGGTAAGTG GAGAATATTA ATAAAGCTTA TATGTAGTGT GCTAGCGTAA TGAAGCACTA 10459 .......... .......... .......... .......... .......... .......... 238 AACCTATGGG TAATGAAACA GATTTGCCCC ATGGGGTACA AGAAACTACC ATAGACAATG 10399 .......... .......... .......... .......... .......... .......... 238 CCCATGGAGA CAAGTATGTC AAATAACTTT TGGGCGAAGG GTTGAATTTG TCCTAAAACT 10339 .......... .......... .......... .......... .......... .......... 238 ATGCGTAATG AAACAGATTT TCCCTCCGTT AATAGTTGGG GTCAAATATG CTCTGGCCGT 10279 .......... .......... .......... .......... .......... .......... 238 TACTAAAATG GGCTAAAATT GCCCTTATTT TCAAATGCCA GAAGCTTACC ACCCGGTGAT 10219 .......... .......... .......... .......... .......... .......... 238 GCCATGTAAT GGTGAGGGCC TAATTGACCT AACTATTAAC GGGAGGGCAA ATATGTTCTA 10159 .......... .......... .......... .......... .......... .......... 238 TTTCACACAG TTTTCGCATA GTTCTAGGTC AAATTTAACC CTTTTCCCAT ATTTTTCTCG 10099 .......... .......... .......... .......... .......... .......... 238 CGGTGATATG AGGCTGGGCC TTTTTCTTTT GATCTTTTGC TGAGTTTCAG ATAGAGTAAT 10039 .......... .......... .......... .......... .......... .......... 238 GTATGACTTG GATAACATGT AAATGTTCGT TAACTAACTT TATGTGTTTG CAGTCATGCA 9979 ||||||| .......... .......... .......... .......... .......... ...TCATGCA 245 ATTCTTAAAT ACCTGGCTTG TGCATTTCCA CGAATTGCAG ATCATTGGTA AGTTCAGCAA 9919 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| ATTCTTAAAT ACCTGGCTTG TGCATTTCCA CGAATTGCAG ATCATTG... .......... 292 GTTTGAGTCT TTCAGTATAT ACCTGAATTG ATGTTCTTTT TAATCATGGA AATGACAATG 9859 .......... .......... .......... .......... .......... .......... 292 AGTACACTTT AAATCTCTTG TGCTTGACAA TGGGTACCCG ACAATAGGCA TTATGAATCA 9799 .......... .......... .......... .......... .......... .......... 292 TCTTTTCTGC ACTTAACATC TCTTGTTTCT TACACCGTCC TATTGAAGGT ATCCAGCTGA 9739 || |||||||||| .......... .......... .......... .......... ........GT ATCCAGCTGA 304 CTTATACAAA 9729 |||||||||| CTTATACAAA 314 hqPGS_C08HBa0251H04.1-_SGN-E245867+ (11478 11376,10926 10853,10740 10680,9985 9932,9750 9729) ******************************************************************************** EST sequence 18 +strand 645 n (File: SGN-E302605+) 1 TACAATTGAT CGGCGCCGGA CTTTGATCAC CGTCGGCGTT TCTAAGCCGT TGCCGGACTA 61 TGCCGGAGCA AATCTCAATC AAGTGAGCTA ACCACTCTGT GTTTCAACTT CTGGTTATAA 121 ATTTTTTAAA GCCTTTGCTC CGTTAGGTTA GGTAGATACG GATCCTATGA CTTCAGAGAT 181 GGAACCAGTT CAGATTAATG GTGCACCTAG GAGACCTCGT ATTCTGCTGG CAGCAAGTGG 241 AAGTGTGGCT TCAATTAAGT TTGCTAATCT ATGTCGTTGT TTTTCTGAAT GGGCAGAAGT 301 TAAAGCAGTT GCAACGAAAC CTTCTCTTCA TTTCATAGAC AAAGCTTCGC TTCCGGAAGA 361 TGTCATTCTT TATACTGATG AGGAGGAATG GTCCACTTGG TGCAGATTGG TGATAGTGTG 421 CTACACATTG AGCTCCGCAG ATGGGCTGAT ATTATGGTTA TTGCCCCTTT GTCAGCAAAC 481 ACACTTGGGA AGATTGCAGG TGGACTATGT GATAACTTGT TAACCTGCAT CGTACGAGCA 541 TGGGACTACA ATAAACCCCT TTTTGTGGCA CCAGCCATGA ATACATTGAT GTGGAATAAT 601 CCATTCACAG AACGACACCT TATGGTAATT GATGAGCTTG GAATC Predicted gene structure (within gDNA segment 12030 to 16985): Exon 1 12630 12700 ( 71 n); cDNA 1 71 ( 71 n); score: 1.000 Intron 1 12701 12847 ( 147 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 12848 12922 ( 75 n); cDNA 72 146 ( 75 n); score: 1.000 Intron 2 12923 15596 (2674 n); Pd: 0.942 (s: 1.00), Pa: 0.983 (s: 1.00) Exon 3 15597 15943 ( 347 n); cDNA 147 492 ( 346 n); score: 0.991 Intron 3 15944 16222 ( 279 n); Pd: 0.999 (s: 1.00), Pa: 0.965 (s: 1.00) Exon 4 16223 16375 ( 153 n); cDNA 493 645 ( 153 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E302605+ 0.995 646 1.002 C PGS_C08HBa0251H04.1+_SGN-E302605+ (12630 12700,12848 12922,15597 15943,16223 16375) Alignment (genomic DNA sequence = upper lines): TACAATTGAT CGGCGCCGGA CTTTGATCAC CGTCGGCGTT TCTAAGCCGT TGCCGGACTA 12689 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACAATTGAT CGGCGCCGGA CTTTGATCAC CGTCGGCGTT TCTAAGCCGT TGCCGGACTA 60 TGCCGGAGCA AGTGAGTTTC AAAAGACTTC AGCCGAATTC ATCGAAAACC TTTTGTTTTC 12749 |||||||||| | TGCCGGAGCA A......... .......... .......... .......... .......... 71 CCCTTAATAT TTTACTTTTT TACTTTCTCC ACATACTGTT TTCATTTTAT TTATTTTTTG 12809 .......... .......... .......... .......... .......... .......... 71 CAATTCATGT GTATTGATAT TGATTATCTC TGTTACAGAT CTCAATCAAG TGAGCTAACC 12869 || |||||||||| |||||||||| .......... .......... .......... ........AT CTCAATCAAG TGAGCTAACC 93 ACTCTGTGTT TCAACTTCTG GTTATAAATT TTTTAAAGCC TTTGCTCCGT TAGGTAAAGT 12929 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| ACTCTGTGTT TCAACTTCTG GTTATAAATT TTTTAAAGCC TTTGCTCCGT TAG....... 146 TTCTGCTCAG TGTCTTACTC TTTACTTTTT TAGCTTTTCA ATTTTGCATT TTTTTTAATC 12989 .......... .......... .......... .......... .......... .......... 146 TCGTGTAATT GCTTAGTTTA ATTATGCAAT AGTGTGCCTC ATGCAGAATA AATTTTTTCA 13049 .......... .......... .......... .......... .......... .......... 146 TGATATTGTT GACCTCCCAA AATAATTCAG TTATAAATGC TTTGTTGTAG AGCTCATGTT 13109 .......... .......... .......... .......... .......... .......... 146 TTTCGCATCC CAAAGCAAAA TCTCAGTATT TTAAGGCAAT TTTCTTCAAT TCTAAGACAA 13169 .......... .......... .......... .......... .......... .......... 146 ACCCAGTTCA ATTCTGTGTT TAATCTTCCT GTTTCATCTG TCTCAAAGCC GATCCGAACA 13229 .......... .......... .......... .......... .......... .......... 146 GTTTTACCCA ATTCTTTGGT TACCTCTTAA GGGTTTTGAG TTGTGGGGTT TAAGATCAGA 13289 .......... .......... .......... .......... .......... .......... 146 TCATACCATG GCTAGTCAAT CCAGCAATCC TTAATCTGTT TATGATTTCA CTGTCAAGGT 13349 .......... .......... .......... .......... .......... .......... 146 TAGAACCTTT GCTTATTTTG ATTTGGGTTT TTTTCTAAAT TGTTCAATTT TGCTTATTTT 13409 .......... .......... .......... .......... .......... .......... 146 GACTGATCGG TTTAGAAAGA ATGTTGTTTT TGTTTGTTTT ATTAGTGTTT GGGGTTTGGA 13469 .......... .......... .......... .......... .......... .......... 146 AATTGAGTTA TGTGGTGTGG TTTTTATCAA CTTTTGGGTT AGTGGGTGAT TGAAATTTGG 13529 .......... .......... .......... .......... .......... .......... 146 TTTATATTGG TTATGAATCA GTAACATAAG CAAAGAATGT GAAAGATCAT TCATTTTTTG 13589 .......... .......... .......... .......... .......... .......... 146 TTTTCTTTTG TGTTAAAGGT TGCTCATTTA TCTATGCTTT TGTTTAGTTT CTTGGTTACC 13649 .......... .......... .......... .......... .......... .......... 146 TCATGAGTTT GAGCCTTGGA AACACGGTAA GGCTGCGTAC GATAGGCTCT TGTGGTACGG 13709 .......... .......... .......... .......... .......... .......... 146 CCCTTCGCCC CTTCCCGAGC TGGAGCATAG TGCATTGGCT TGCCTTTTGT TTTTTGCAAT 13769 .......... .......... .......... .......... .......... .......... 146 TAGAGATTTG GGGCTTTAAG TTCTGAGGAG TTATTTTCAA CTTATTAGCC CTTGCTTTAT 13829 .......... .......... .......... .......... .......... .......... 146 TGGTTTGTAT AGATTTGGAA TGACAAGATA GTGTAAAATA TGGTCTTTTC TATTCACTTC 13889 .......... .......... .......... .......... .......... .......... 146 ATCTTTTGAG GTAAAGAGAG ATGCATCTGC TGTGTAGGTA GCTAGGTTAA CTTGTTACAT 13949 .......... .......... .......... .......... .......... .......... 146 GAATTTGGTT GATCTAGACA AGCATCTGTA TTGCAAACAC TTTCCAACCA TTTAAGTGTT 14009 .......... .......... .......... .......... .......... .......... 146 ATAGTGCAAA TGATTTTGAT TTACCAGAGA GGGAAAAAAA AAAAGATAAG CAGAGGGATG 14069 .......... .......... .......... .......... .......... .......... 146 TGTGTGAGCT TGTGATATAT GGTACCAAAT TGGTGCTTGA AGCAAACTAA GGCTAGTTGC 14129 .......... .......... .......... .......... .......... .......... 146 TGCATTTATC ATCTAAACTT GGGGCTTGTT TTGTTTCTTT GGACTTGCTT CACAATCATA 14189 .......... .......... .......... .......... .......... .......... 146 ACTATTTGGA CTTAACTCAC CCTAGGTGTG GCCAAATCAA TGCTTAGCTG AATTTCTCAA 14249 .......... .......... .......... .......... .......... .......... 146 AACCAATTTT AGTCCTCATC TTCACAGACA TCAAAAACAG TTGGCCACCA GCTAATTAGC 14309 .......... .......... .......... .......... .......... .......... 146 TGATGTAGAT ACACAGATTT GAACTTTGAC TTGCTTCATG ACATGTTCAC CTGAACTTTT 14369 .......... .......... .......... .......... .......... .......... 146 AGTTGGTCCT TAATTCGGTT AAATTGGTTT TGCTGGTCTT TCATTTTGCT GAACTAGCTT 14429 .......... .......... .......... .......... .......... .......... 146 TCAGCTCGAG TAAACTGACC TCAACTCGGC ATATCTTTTT TTTTTTTGCC TAGCTTAAAA 14489 .......... .......... .......... .......... .......... .......... 146 TAAGCTACTT GTTCTTGTAG AGGACTAGTT GTTGACAGAG TCATTGACCG TAACAACCTT 14549 .......... .......... .......... .......... .......... .......... 146 TCATTAGATT TGATGGAACT TTGTAGTTCT GCTTCCATCA TGGTAATTCT AGATTTCTCA 14609 .......... .......... .......... .......... .......... .......... 146 CATTTGAAGG TGAGTTTCAA ACTTGTCTTG AGGCTCATGG ATTGGATCCA AGTACCACCA 14669 .......... .......... .......... .......... .......... .......... 146 TCAGTTTCTT TCTCAAGTGT TGTGGGCTTG TCAAATTAGT CAATTTCTTT AACTGCTATT 14729 .......... .......... .......... .......... .......... .......... 146 TGGGATGAAT TACTTATTTC CACCTTAAAT CAATTCAATA TTGAATATGA ACAGTCAATA 14789 .......... .......... .......... .......... .......... .......... 146 GAAATACCTA ATTGTTGATA AATTAGGGTT TTTGTAAGAG AAAATAAGAT GGGTAGTGAA 14849 .......... .......... .......... .......... .......... .......... 146 TTGTTGATAA AGTTGGAATA TTTTGGTATT CCTATAGACT GTTCATATTC AATATTGAAT 14909 .......... .......... .......... .......... .......... .......... 146 TGATTTTGAG GTGGAAATAG GTAATTCATC CCAACTAGTT GTTAAAGAAA TTGATTAATT 14969 .......... .......... .......... .......... .......... .......... 146 TGGCAAGCCC ATAACACTTG AGAAAGAAAC TGATGGTGGT TACTTGGATC CAATCCATGA 15029 .......... .......... .......... .......... .......... .......... 146 GCCTCAAGAC AAGTTTGAAA CTCACCTTCA AATGTGAGAA ATCCAGAATT ACCATGATGG 15089 .......... .......... .......... .......... .......... .......... 146 AAGCAGAACT ACAAAATTCC ATCAAATCTA AGGAAGGTTG TTAAGGTCAA TGAATCTGTC 15149 .......... .......... .......... .......... .......... .......... 146 AAAATCTAGT CCTCTACAAG AACAAGTAGC TTATTTTACC CTAGGCAAAA AAAAAAAGAT 15209 .......... .......... .......... .......... .......... .......... 146 GCCGAGTTGA GGTCACTTTA CTCGAGCTGA AGGCTAGTTC AGCAGAATGA AAGACCAGCA 15269 .......... .......... .......... .......... .......... .......... 146 AACCGAATTA AGGACCAATT AACCAGTTCA AGTGAACATG TCAGTAAGCA AGTCAAAGTT 15329 .......... .......... .......... .......... .......... .......... 146 CAAATCCGTG TATCTACATC AGCTAACCAG CTGGTGGCCA ATTTTTTTTG ATGTCTATGA 15389 .......... .......... .......... .......... .......... .......... 146 AGATGAGGGC TAAATGAGAA ATTAAGATAA GCATTGATTT GGTCACACCT AGGGTGAGTT 15449 .......... .......... .......... .......... .......... .......... 146 AAGTCCAAAG AGATGATTGT GAAGCAAGTT ACCTGAGGGC AGATCTCTTA ATTATTTACT 15509 .......... .......... .......... .......... .......... .......... 146 GTTGTTGTTC CCCTTAGTTG CACGCATGTA CAATTACTTC TATCTTTCTC TTAGTGCAGT 15569 .......... .......... .......... .......... .......... .......... 146 TAAAGAATCC TTTTATATGT ATCACAGGTT AGGTAGATAC GGATCCTATG ACTTCAGAGA 15629 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......GTT AGGTAGATAC GGATCCTATG ACTTCAGAGA 179 TGGAACCAGT TCAGATTAAT GGTGCACCTA GGAGACCTCG TATTCTGCTG GCAGCAAGTG 15689 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGAACCAGT TCAGATTAAT GGTGCACCTA GGAGACCTCG TATTCTGCTG GCAGCAAGTG 239 GAAGTGTGGC TTCAATTAAG TTTGCTAATC TATGTCGTTG TTTTTCTGAA TGGGCAGAAG 15749 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGTGGC TTCAATTAAG TTTGCTAATC TATGTCGTTG TTTTTCTGAA TGGGCAGAAG 299 TTAAAGCAGT TGCAACGAAA CCTTCTCTTC ATTTCATAGA CAAAGCTTCG CTTCCGGAAG 15809 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAAAGCAGT TGCAACGAAA CCTTCTCTTC ATTTCATAGA CAAAGCTTCG CTTCCGGAAG 359 ATGTCATTCT TTATACTGAT GAGGAGGAAT GGTCCACTTG GAAGAAGATT GGTGATAGTG 15869 |||||||||| |||||||||| |||||||||| |||||||||| | | ||||| |||||||||| ATGTCATTCT TTATACTGAT GAGGAGGAAT GGTCCACTTG G-TGCAGATT GGTGATAGTG 418 TGCTACACAT TGAGCTCCGC AGATGGGCTG ATATTATGGT TATTGCCCCT TTGTCAGCAA 15929 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTACACAT TGAGCTCCGC AGATGGGCTG ATATTATGGT TATTGCCCCT TTGTCAGCAA 478 ACACACTTGG GAAGGTGATG CATCATTCCA TGTTTTATTA CTGAAATTTT ATTTTATGAT 15989 |||||||||| |||| ACACACTTGG GAAG...... .......... .......... .......... .......... 492 AACTCTCATT CTGCCTCAAA AACACAATTT ATTCCCTTAT CCTGTACTAA TTGATCTTTA 16049 .......... .......... .......... .......... .......... .......... 492 TGAAATGGAC TATCAACAAT GTTAAACATA CTAATTGAAC ATGTCCATGC TAGAAGATTA 16109 .......... .......... .......... .......... .......... .......... 492 ACGTTAAGAA TGACACCAAT TCAATTCCTT ACATCATTAA GAAAAAGTTT GTAGTATTAC 16169 .......... .......... .......... .......... .......... .......... 492 TTATATTGCG ATCTACTTTG CAATTAAGCT GATATATGTC TTATGTACTG TAGATTGCAG 16229 ||||||| .......... .......... .......... .......... .......... ...ATTGCAG 499 GTGGACTATG TGATAACTTG TTAACCTGCA TCGTACGAGC ATGGGACTAC AATAAACCCC 16289 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGGACTATG TGATAACTTG TTAACCTGCA TCGTACGAGC ATGGGACTAC AATAAACCCC 559 TTTTTGTGGC ACCAGCCATG AATACATTGA TGTGGAATAA TCCATTCACA GAACGACACC 16349 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTGTGGC ACCAGCCATG AATACATTGA TGTGGAATAA TCCATTCACA GAACGACACC 619 TTATGGTAAT TGATGAGCTT GGAATC 16375 |||||||||| |||||||||| |||||| TTATGGTAAT TGATGAGCTT GGAATC 645 hqPGS_C08HBa0251H04.1+_SGN-E302605+ (12630 12700,12848 12922,15597 15943,16223 16375) ******************************************************************************** EST sequence 39 +strand 447 n (File: SGN-E283812+) 1 ATCGGCGCCG GACTTTGATC ACCGTCGGCG TTTCTAAGCC GTTGCCGGAC TATGCCGGAG 61 CAAATCTCAA TCAAGTGAGC TAACCACTCT GTGTTTCAAC TTCTGGTTAT AAATTTTTTA 121 AAGCCTTTGC TCCGTTAGGT TAGGTAGATA CGGATCCTAT GACTTCAGAG ATGGAACCAG 181 TTCAGATTAA TGGTGCACCT AGGAGACCTC GTATTCTGCT GGCAGCAAGT GGAAGTGTGG 241 CTTCAATTAA GTTTGCTAAT CTATGTCGTT GTTTTTCTGA ATGGGCAGAA GTTAAAGCAG 301 TTGCAACGAA ACCTTCTCTT CATTTCATAG ACAAAGCTTC GCTTCCGGAA GATGTCATTC 361 TTTATACTGA TGAGGAGGAA TGGTCCACTT GGAAGAAGAT TGGTGATAGT GTGCTACACA 421 TTGAGCTCCG CAGATGGGCT GATATTA Predicted gene structure (within gDNA segment 12038 to 16515): Exon 1 12638 12700 ( 63 n); cDNA 1 63 ( 63 n); score: 1.000 Intron 1 12701 12847 ( 147 n); Pd: 0.000 (s: 1.00), Pa: 0.990 (s: 1.00) Exon 2 12848 12922 ( 75 n); cDNA 64 138 ( 75 n); score: 1.000 Intron 2 12923 15596 (2674 n); Pd: 0.942 (s: 1.00), Pa: 0.983 (s: 1.00) Exon 3 15597 15905 ( 309 n); cDNA 139 447 ( 309 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E283812+ 1.000 447 1.000 C PGS_C08HBa0251H04.1+_SGN-E283812+ (12638 12700,12848 12922,15597 15905) Alignment (genomic DNA sequence = upper lines): ATCGGCGCCG GACTTTGATC ACCGTCGGCG TTTCTAAGCC GTTGCCGGAC TATGCCGGAG 12697 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCGGCGCCG GACTTTGATC ACCGTCGGCG TTTCTAAGCC GTTGCCGGAC TATGCCGGAG 60 CAAGTGAGTT TCAAAAGACT TCAGCCGAAT TCATCGAAAA CCTTTTGTTT TCCCCTTAAT 12757 ||| CAA....... .......... .......... .......... .......... .......... 63 ATTTTACTTT TTTACTTTCT CCACATACTG TTTTCATTTT ATTTATTTTT TGCAATTCAT 12817 .......... .......... .......... .......... .......... .......... 63 GTGTATTGAT ATTGATTATC TCTGTTACAG ATCTCAATCA AGTGAGCTAA CCACTCTGTG 12877 |||||||||| |||||||||| |||||||||| .......... .......... .......... ATCTCAATCA AGTGAGCTAA CCACTCTGTG 93 TTTCAACTTC TGGTTATAAA TTTTTTAAAG CCTTTGCTCC GTTAGGTAAA GTTTCTGCTC 12937 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TTTCAACTTC TGGTTATAAA TTTTTTAAAG CCTTTGCTCC GTTAG..... .......... 138 AGTGTCTTAC TCTTTACTTT TTTAGCTTTT CAATTTTGCA TTTTTTTTAA TCTCGTGTAA 12997 .......... .......... .......... .......... .......... .......... 138 TTGCTTAGTT TAATTATGCA ATAGTGTGCC TCATGCAGAA TAAATTTTTT CATGATATTG 13057 .......... .......... .......... .......... .......... .......... 138 TTGACCTCCC AAAATAATTC AGTTATAAAT GCTTTGTTGT AGAGCTCATG TTTTTCGCAT 13117 .......... .......... .......... .......... .......... .......... 138 CCCAAAGCAA AATCTCAGTA TTTTAAGGCA ATTTTCTTCA ATTCTAAGAC AAACCCAGTT 13177 .......... .......... .......... .......... .......... .......... 138 CAATTCTGTG TTTAATCTTC CTGTTTCATC TGTCTCAAAG CCGATCCGAA CAGTTTTACC 13237 .......... .......... .......... .......... .......... .......... 138 CAATTCTTTG GTTACCTCTT AAGGGTTTTG AGTTGTGGGG TTTAAGATCA GATCATACCA 13297 .......... .......... .......... .......... .......... .......... 138 TGGCTAGTCA ATCCAGCAAT CCTTAATCTG TTTATGATTT CACTGTCAAG GTTAGAACCT 13357 .......... .......... .......... .......... .......... .......... 138 TTGCTTATTT TGATTTGGGT TTTTTTCTAA ATTGTTCAAT TTTGCTTATT TTGACTGATC 13417 .......... .......... .......... .......... .......... .......... 138 GGTTTAGAAA GAATGTTGTT TTTGTTTGTT TTATTAGTGT TTGGGGTTTG GAAATTGAGT 13477 .......... .......... .......... .......... .......... .......... 138 TATGTGGTGT GGTTTTTATC AACTTTTGGG TTAGTGGGTG ATTGAAATTT GGTTTATATT 13537 .......... .......... .......... .......... .......... .......... 138 GGTTATGAAT CAGTAACATA AGCAAAGAAT GTGAAAGATC ATTCATTTTT TGTTTTCTTT 13597 .......... .......... .......... .......... .......... .......... 138 TGTGTTAAAG GTTGCTCATT TATCTATGCT TTTGTTTAGT TTCTTGGTTA CCTCATGAGT 13657 .......... .......... .......... .......... .......... .......... 138 TTGAGCCTTG GAAACACGGT AAGGCTGCGT ACGATAGGCT CTTGTGGTAC GGCCCTTCGC 13717 .......... .......... .......... .......... .......... .......... 138 CCCTTCCCGA GCTGGAGCAT AGTGCATTGG CTTGCCTTTT GTTTTTTGCA ATTAGAGATT 13777 .......... .......... .......... .......... .......... .......... 138 TGGGGCTTTA AGTTCTGAGG AGTTATTTTC AACTTATTAG CCCTTGCTTT ATTGGTTTGT 13837 .......... .......... .......... .......... .......... .......... 138 ATAGATTTGG AATGACAAGA TAGTGTAAAA TATGGTCTTT TCTATTCACT TCATCTTTTG 13897 .......... .......... .......... .......... .......... .......... 138 AGGTAAAGAG AGATGCATCT GCTGTGTAGG TAGCTAGGTT AACTTGTTAC ATGAATTTGG 13957 .......... .......... .......... .......... .......... .......... 138 TTGATCTAGA CAAGCATCTG TATTGCAAAC ACTTTCCAAC CATTTAAGTG TTATAGTGCA 14017 .......... .......... .......... .......... .......... .......... 138 AATGATTTTG ATTTACCAGA GAGGGAAAAA AAAAAAGATA AGCAGAGGGA TGTGTGTGAG 14077 .......... .......... .......... .......... .......... .......... 138 CTTGTGATAT ATGGTACCAA ATTGGTGCTT GAAGCAAACT AAGGCTAGTT GCTGCATTTA 14137 .......... .......... .......... .......... .......... .......... 138 TCATCTAAAC TTGGGGCTTG TTTTGTTTCT TTGGACTTGC TTCACAATCA TAACTATTTG 14197 .......... .......... .......... .......... .......... .......... 138 GACTTAACTC ACCCTAGGTG TGGCCAAATC AATGCTTAGC TGAATTTCTC AAAACCAATT 14257 .......... .......... .......... .......... .......... .......... 138 TTAGTCCTCA TCTTCACAGA CATCAAAAAC AGTTGGCCAC CAGCTAATTA GCTGATGTAG 14317 .......... .......... .......... .......... .......... .......... 138 ATACACAGAT TTGAACTTTG ACTTGCTTCA TGACATGTTC ACCTGAACTT TTAGTTGGTC 14377 .......... .......... .......... .......... .......... .......... 138 CTTAATTCGG TTAAATTGGT TTTGCTGGTC TTTCATTTTG CTGAACTAGC TTTCAGCTCG 14437 .......... .......... .......... .......... .......... .......... 138 AGTAAACTGA CCTCAACTCG GCATATCTTT TTTTTTTTTG CCTAGCTTAA AATAAGCTAC 14497 .......... .......... .......... .......... .......... .......... 138 TTGTTCTTGT AGAGGACTAG TTGTTGACAG AGTCATTGAC CGTAACAACC TTTCATTAGA 14557 .......... .......... .......... .......... .......... .......... 138 TTTGATGGAA CTTTGTAGTT CTGCTTCCAT CATGGTAATT CTAGATTTCT CACATTTGAA 14617 .......... .......... .......... .......... .......... .......... 138 GGTGAGTTTC AAACTTGTCT TGAGGCTCAT GGATTGGATC CAAGTACCAC CATCAGTTTC 14677 .......... .......... .......... .......... .......... .......... 138 TTTCTCAAGT GTTGTGGGCT TGTCAAATTA GTCAATTTCT TTAACTGCTA TTTGGGATGA 14737 .......... .......... .......... .......... .......... .......... 138 ATTACTTATT TCCACCTTAA ATCAATTCAA TATTGAATAT GAACAGTCAA TAGAAATACC 14797 .......... .......... .......... .......... .......... .......... 138 TAATTGTTGA TAAATTAGGG TTTTTGTAAG AGAAAATAAG ATGGGTAGTG AATTGTTGAT 14857 .......... .......... .......... .......... .......... .......... 138 AAAGTTGGAA TATTTTGGTA TTCCTATAGA CTGTTCATAT TCAATATTGA ATTGATTTTG 14917 .......... .......... .......... .......... .......... .......... 138 AGGTGGAAAT AGGTAATTCA TCCCAACTAG TTGTTAAAGA AATTGATTAA TTTGGCAAGC 14977 .......... .......... .......... .......... .......... .......... 138 CCATAACACT TGAGAAAGAA ACTGATGGTG GTTACTTGGA TCCAATCCAT GAGCCTCAAG 15037 .......... .......... .......... .......... .......... .......... 138 ACAAGTTTGA AACTCACCTT CAAATGTGAG AAATCCAGAA TTACCATGAT GGAAGCAGAA 15097 .......... .......... .......... .......... .......... .......... 138 CTACAAAATT CCATCAAATC TAAGGAAGGT TGTTAAGGTC AATGAATCTG TCAAAATCTA 15157 .......... .......... .......... .......... .......... .......... 138 GTCCTCTACA AGAACAAGTA GCTTATTTTA CCCTAGGCAA AAAAAAAAAG ATGCCGAGTT 15217 .......... .......... .......... .......... .......... .......... 138 GAGGTCACTT TACTCGAGCT GAAGGCTAGT TCAGCAGAAT GAAAGACCAG CAAACCGAAT 15277 .......... .......... .......... .......... .......... .......... 138 TAAGGACCAA TTAACCAGTT CAAGTGAACA TGTCAGTAAG CAAGTCAAAG TTCAAATCCG 15337 .......... .......... .......... .......... .......... .......... 138 TGTATCTACA TCAGCTAACC AGCTGGTGGC CAATTTTTTT TGATGTCTAT GAAGATGAGG 15397 .......... .......... .......... .......... .......... .......... 138 GCTAAATGAG AAATTAAGAT AAGCATTGAT TTGGTCACAC CTAGGGTGAG TTAAGTCCAA 15457 .......... .......... .......... .......... .......... .......... 138 AGAGATGATT GTGAAGCAAG TTACCTGAGG GCAGATCTCT TAATTATTTA CTGTTGTTGT 15517 .......... .......... .......... .......... .......... .......... 138 TCCCCTTAGT TGCACGCATG TACAATTACT TCTATCTTTC TCTTAGTGCA GTTAAAGAAT 15577 .......... .......... .......... .......... .......... .......... 138 CCTTTTATAT GTATCACAGG TTAGGTAGAT ACGGATCCTA TGACTTCAGA GATGGAACCA 15637 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........G TTAGGTAGAT ACGGATCCTA TGACTTCAGA GATGGAACCA 179 GTTCAGATTA ATGGTGCACC TAGGAGACCT CGTATTCTGC TGGCAGCAAG TGGAAGTGTG 15697 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTCAGATTA ATGGTGCACC TAGGAGACCT CGTATTCTGC TGGCAGCAAG TGGAAGTGTG 239 GCTTCAATTA AGTTTGCTAA TCTATGTCGT TGTTTTTCTG AATGGGCAGA AGTTAAAGCA 15757 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTCAATTA AGTTTGCTAA TCTATGTCGT TGTTTTTCTG AATGGGCAGA AGTTAAAGCA 299 GTTGCAACGA AACCTTCTCT TCATTTCATA GACAAAGCTT CGCTTCCGGA AGATGTCATT 15817 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTGCAACGA AACCTTCTCT TCATTTCATA GACAAAGCTT CGCTTCCGGA AGATGTCATT 359 CTTTATACTG ATGAGGAGGA ATGGTCCACT TGGAAGAAGA TTGGTGATAG TGTGCTACAC 15877 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTTATACTG ATGAGGAGGA ATGGTCCACT TGGAAGAAGA TTGGTGATAG TGTGCTACAC 419 ATTGAGCTCC GCAGATGGGC TGATATTA 15905 |||||||||| |||||||||| |||||||| ATTGAGCTCC GCAGATGGGC TGATATTA 447 hqPGS_C08HBa0251H04.1+_SGN-E283812+ (12638 12700,12848 12922,15597 15905) ******************************************************************************** EST sequence 60 +strand 795 n (File: SGN-E349881+) 1 GCCGGAGCAA ATCTCAATCA AGTGAGCTAA CCACTCTGTG TTTCAACTTC TGGTTATAAA 61 TTTTTTAAAG CCTTTGCTCC GTTAGGTTAG GTAGATACGG ATCCTATGAC TTCAGAGATG 121 GAACCAGTTC AGATTAATGG TGCACCTAGG AGACCTCGTA TTCTGCTGGC AGCAAGTGGA 181 AGTGTGGCTT CAATTAAGTT TGCTAATCTA TGTCGTTGTT TTTCTGAATG GGCAGAAGTT 241 AAAGCAGTTG CAACGAAACC TTCTCTTCAT TTCATAGACA AAGCTTCGCT TCCGGAAGAT 301 GTCATTCTTT ATACTGATGA GGAGGAATGG TCCACTTGGA AGAAGATTGG TGATAGTGTG 361 CTACACATTG AGCTCCGCAG ATGGGCTGAT ATTATGGTTA TTGCCCCTTT GTCAGCAAAC 421 ACACTTGGGA AGATTGCAGG TGGACTATGT GATAACTTGT TAACCTGCAT CGTACGAGCA 481 TGGGACTACA ATAAACCCCT TTTTGTGGCA CCAGCCATGA ATACATTGAT GTGGAATAAT 541 CCATTCACAG AACGACACCT TATGGTAATT GATGAGCTTG GAATCTCTCT CATACCACCA 601 GTTTCAAAAA GACTAGCATG TGGAGATTAT GGAAATGGCG CTATGGCTGA ACCTTCTCTC 661 ATCTACTCAA CTGTAAGACT CTTCTATGAG TCACGGTCAC AATCAGGTGG CATCAACTTG 721 GCTTGATTCG CAGATCATTA AATTTTATTC ATCGGTTTGT ACAAGTGGTA GAAATTGTTG 781 AAATCAGGAC CTGGA Predicted gene structure (within gDNA segment 12148 to 17294): Exon 1 12691 12700 ( 10 n); cDNA 1 10 ( 10 n); score: 1.000 Intron 1 12701 12847 ( 147 n); Pd: 0.000 (s: 0), Pa: 0.990 (s: 1.00) Exon 2 12848 12922 ( 75 n); cDNA 11 85 ( 75 n); score: 1.000 Intron 2 12923 15596 (2674 n); Pd: 0.942 (s: 1.00), Pa: 0.983 (s: 1.00) Exon 3 15597 15943 ( 347 n); cDNA 86 432 ( 347 n); score: 1.000 Intron 3 15944 16222 ( 279 n); Pd: 0.999 (s: 1.00), Pa: 0.965 (s: 1.00) Exon 4 16223 16586 ( 364 n); cDNA 433 795 ( 363 n); score: 0.997 MATCH C08HBa0251H04.1+ SGN-E349881+ 0.999 796 1.001 C PGS_C08HBa0251H04.1+_SGN-E349881+ (12691 12700,12848 12922,15597 15943,16223 16586) Alignment (genomic DNA sequence = upper lines): GCCGGAGCAA GTGAGTTTCA AAAGACTTCA GCCGAATTCA TCGAAAACCT TTTGTTTTCC 12750 |||||||||| GCCGGAGCAA .......... .......... .......... .......... .......... 10 CCTTAATATT TTACTTTTTT ACTTTCTCCA CATACTGTTT TCATTTTATT TATTTTTTGC 12810 .......... .......... .......... .......... .......... .......... 10 AATTCATGTG TATTGATATT GATTATCTCT GTTACAGATC TCAATCAAGT GAGCTAACCA 12870 ||| |||||||||| |||||||||| .......... .......... .......... .......ATC TCAATCAAGT GAGCTAACCA 33 CTCTGTGTTT CAACTTCTGG TTATAAATTT TTTAAAGCCT TTGCTCCGTT AGGTAAAGTT 12930 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || CTCTGTGTTT CAACTTCTGG TTATAAATTT TTTAAAGCCT TTGCTCCGTT AG........ 85 TCTGCTCAGT GTCTTACTCT TTACTTTTTT AGCTTTTCAA TTTTGCATTT TTTTTAATCT 12990 .......... .......... .......... .......... .......... .......... 85 CGTGTAATTG CTTAGTTTAA TTATGCAATA GTGTGCCTCA TGCAGAATAA ATTTTTTCAT 13050 .......... .......... .......... .......... .......... .......... 85 GATATTGTTG ACCTCCCAAA ATAATTCAGT TATAAATGCT TTGTTGTAGA GCTCATGTTT 13110 .......... .......... .......... .......... .......... .......... 85 TTCGCATCCC AAAGCAAAAT CTCAGTATTT TAAGGCAATT TTCTTCAATT CTAAGACAAA 13170 .......... .......... .......... .......... .......... .......... 85 CCCAGTTCAA TTCTGTGTTT AATCTTCCTG TTTCATCTGT CTCAAAGCCG ATCCGAACAG 13230 .......... .......... .......... .......... .......... .......... 85 TTTTACCCAA TTCTTTGGTT ACCTCTTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 13290 .......... .......... .......... .......... .......... .......... 85 CATACCATGG CTAGTCAATC CAGCAATCCT TAATCTGTTT ATGATTTCAC TGTCAAGGTT 13350 .......... .......... .......... .......... .......... .......... 85 AGAACCTTTG CTTATTTTGA TTTGGGTTTT TTTCTAAATT GTTCAATTTT GCTTATTTTG 13410 .......... .......... .......... .......... .......... .......... 85 ACTGATCGGT TTAGAAAGAA TGTTGTTTTT GTTTGTTTTA TTAGTGTTTG GGGTTTGGAA 13470 .......... .......... .......... .......... .......... .......... 85 ATTGAGTTAT GTGGTGTGGT TTTTATCAAC TTTTGGGTTA GTGGGTGATT GAAATTTGGT 13530 .......... .......... .......... .......... .......... .......... 85 TTATATTGGT TATGAATCAG TAACATAAGC AAAGAATGTG AAAGATCATT CATTTTTTGT 13590 .......... .......... .......... .......... .......... .......... 85 TTTCTTTTGT GTTAAAGGTT GCTCATTTAT CTATGCTTTT GTTTAGTTTC TTGGTTACCT 13650 .......... .......... .......... .......... .......... .......... 85 CATGAGTTTG AGCCTTGGAA ACACGGTAAG GCTGCGTACG ATAGGCTCTT GTGGTACGGC 13710 .......... .......... .......... .......... .......... .......... 85 CCTTCGCCCC TTCCCGAGCT GGAGCATAGT GCATTGGCTT GCCTTTTGTT TTTTGCAATT 13770 .......... .......... .......... .......... .......... .......... 85 AGAGATTTGG GGCTTTAAGT TCTGAGGAGT TATTTTCAAC TTATTAGCCC TTGCTTTATT 13830 .......... .......... .......... .......... .......... .......... 85 GGTTTGTATA GATTTGGAAT GACAAGATAG TGTAAAATAT GGTCTTTTCT ATTCACTTCA 13890 .......... .......... .......... .......... .......... .......... 85 TCTTTTGAGG TAAAGAGAGA TGCATCTGCT GTGTAGGTAG CTAGGTTAAC TTGTTACATG 13950 .......... .......... .......... .......... .......... .......... 85 AATTTGGTTG ATCTAGACAA GCATCTGTAT TGCAAACACT TTCCAACCAT TTAAGTGTTA 14010 .......... .......... .......... .......... .......... .......... 85 TAGTGCAAAT GATTTTGATT TACCAGAGAG GGAAAAAAAA AAAGATAAGC AGAGGGATGT 14070 .......... .......... .......... .......... .......... .......... 85 GTGTGAGCTT GTGATATATG GTACCAAATT GGTGCTTGAA GCAAACTAAG GCTAGTTGCT 14130 .......... .......... .......... .......... .......... .......... 85 GCATTTATCA TCTAAACTTG GGGCTTGTTT TGTTTCTTTG GACTTGCTTC ACAATCATAA 14190 .......... .......... .......... .......... .......... .......... 85 CTATTTGGAC TTAACTCACC CTAGGTGTGG CCAAATCAAT GCTTAGCTGA ATTTCTCAAA 14250 .......... .......... .......... .......... .......... .......... 85 ACCAATTTTA GTCCTCATCT TCACAGACAT CAAAAACAGT TGGCCACCAG CTAATTAGCT 14310 .......... .......... .......... .......... .......... .......... 85 GATGTAGATA CACAGATTTG AACTTTGACT TGCTTCATGA CATGTTCACC TGAACTTTTA 14370 .......... .......... .......... .......... .......... .......... 85 GTTGGTCCTT AATTCGGTTA AATTGGTTTT GCTGGTCTTT CATTTTGCTG AACTAGCTTT 14430 .......... .......... .......... .......... .......... .......... 85 CAGCTCGAGT AAACTGACCT CAACTCGGCA TATCTTTTTT TTTTTTGCCT AGCTTAAAAT 14490 .......... .......... .......... .......... .......... .......... 85 AAGCTACTTG TTCTTGTAGA GGACTAGTTG TTGACAGAGT CATTGACCGT AACAACCTTT 14550 .......... .......... .......... .......... .......... .......... 85 CATTAGATTT GATGGAACTT TGTAGTTCTG CTTCCATCAT GGTAATTCTA GATTTCTCAC 14610 .......... .......... .......... .......... .......... .......... 85 ATTTGAAGGT GAGTTTCAAA CTTGTCTTGA GGCTCATGGA TTGGATCCAA GTACCACCAT 14670 .......... .......... .......... .......... .......... .......... 85 CAGTTTCTTT CTCAAGTGTT GTGGGCTTGT CAAATTAGTC AATTTCTTTA ACTGCTATTT 14730 .......... .......... .......... .......... .......... .......... 85 GGGATGAATT ACTTATTTCC ACCTTAAATC AATTCAATAT TGAATATGAA CAGTCAATAG 14790 .......... .......... .......... .......... .......... .......... 85 AAATACCTAA TTGTTGATAA ATTAGGGTTT TTGTAAGAGA AAATAAGATG GGTAGTGAAT 14850 .......... .......... .......... .......... .......... .......... 85 TGTTGATAAA GTTGGAATAT TTTGGTATTC CTATAGACTG TTCATATTCA ATATTGAATT 14910 .......... .......... .......... .......... .......... .......... 85 GATTTTGAGG TGGAAATAGG TAATTCATCC CAACTAGTTG TTAAAGAAAT TGATTAATTT 14970 .......... .......... .......... .......... .......... .......... 85 GGCAAGCCCA TAACACTTGA GAAAGAAACT GATGGTGGTT ACTTGGATCC AATCCATGAG 15030 .......... .......... .......... .......... .......... .......... 85 CCTCAAGACA AGTTTGAAAC TCACCTTCAA ATGTGAGAAA TCCAGAATTA CCATGATGGA 15090 .......... .......... .......... .......... .......... .......... 85 AGCAGAACTA CAAAATTCCA TCAAATCTAA GGAAGGTTGT TAAGGTCAAT GAATCTGTCA 15150 .......... .......... .......... .......... .......... .......... 85 AAATCTAGTC CTCTACAAGA ACAAGTAGCT TATTTTACCC TAGGCAAAAA AAAAAAGATG 15210 .......... .......... .......... .......... .......... .......... 85 CCGAGTTGAG GTCACTTTAC TCGAGCTGAA GGCTAGTTCA GCAGAATGAA AGACCAGCAA 15270 .......... .......... .......... .......... .......... .......... 85 ACCGAATTAA GGACCAATTA ACCAGTTCAA GTGAACATGT CAGTAAGCAA GTCAAAGTTC 15330 .......... .......... .......... .......... .......... .......... 85 AAATCCGTGT ATCTACATCA GCTAACCAGC TGGTGGCCAA TTTTTTTTGA TGTCTATGAA 15390 .......... .......... .......... .......... .......... .......... 85 GATGAGGGCT AAATGAGAAA TTAAGATAAG CATTGATTTG GTCACACCTA GGGTGAGTTA 15450 .......... .......... .......... .......... .......... .......... 85 AGTCCAAAGA GATGATTGTG AAGCAAGTTA CCTGAGGGCA GATCTCTTAA TTATTTACTG 15510 .......... .......... .......... .......... .......... .......... 85 TTGTTGTTCC CCTTAGTTGC ACGCATGTAC AATTACTTCT ATCTTTCTCT TAGTGCAGTT 15570 .......... .......... .......... .......... .......... .......... 85 AAAGAATCCT TTTATATGTA TCACAGGTTA GGTAGATACG GATCCTATGA CTTCAGAGAT 15630 |||| |||||||||| |||||||||| |||||||||| .......... .......... ......GTTA GGTAGATACG GATCCTATGA CTTCAGAGAT 119 GGAACCAGTT CAGATTAATG GTGCACCTAG GAGACCTCGT ATTCTGCTGG CAGCAAGTGG 15690 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAACCAGTT CAGATTAATG GTGCACCTAG GAGACCTCGT ATTCTGCTGG CAGCAAGTGG 179 AAGTGTGGCT TCAATTAAGT TTGCTAATCT ATGTCGTTGT TTTTCTGAAT GGGCAGAAGT 15750 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGTGGCT TCAATTAAGT TTGCTAATCT ATGTCGTTGT TTTTCTGAAT GGGCAGAAGT 239 TAAAGCAGTT GCAACGAAAC CTTCTCTTCA TTTCATAGAC AAAGCTTCGC TTCCGGAAGA 15810 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAAGCAGTT GCAACGAAAC CTTCTCTTCA TTTCATAGAC AAAGCTTCGC TTCCGGAAGA 299 TGTCATTCTT TATACTGATG AGGAGGAATG GTCCACTTGG AAGAAGATTG GTGATAGTGT 15870 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTCATTCTT TATACTGATG AGGAGGAATG GTCCACTTGG AAGAAGATTG GTGATAGTGT 359 GCTACACATT GAGCTCCGCA GATGGGCTGA TATTATGGTT ATTGCCCCTT TGTCAGCAAA 15930 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTACACATT GAGCTCCGCA GATGGGCTGA TATTATGGTT ATTGCCCCTT TGTCAGCAAA 419 CACACTTGGG AAGGTGATGC ATCATTCCAT GTTTTATTAC TGAAATTTTA TTTTATGATA 15990 |||||||||| ||| CACACTTGGG AAG....... .......... .......... .......... .......... 432 ACTCTCATTC TGCCTCAAAA ACACAATTTA TTCCCTTATC CTGTACTAAT TGATCTTTAT 16050 .......... .......... .......... .......... .......... .......... 432 GAAATGGACT ATCAACAATG TTAAACATAC TAATTGAACA TGTCCATGCT AGAAGATTAA 16110 .......... .......... .......... .......... .......... .......... 432 CGTTAAGAAT GACACCAATT CAATTCCTTA CATCATTAAG AAAAAGTTTG TAGTATTACT 16170 .......... .......... .......... .......... .......... .......... 432 TATATTGCGA TCTACTTTGC AATTAAGCTG ATATATGTCT TATGTACTGT AGATTGCAGG 16230 |||||||| .......... .......... .......... .......... .......... ..ATTGCAGG 440 TGGACTATGT GATAACTTGT TAACCTGCAT CGTACGAGCA TGGGACTACA ATAAACCCCT 16290 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGACTATGT GATAACTTGT TAACCTGCAT CGTACGAGCA TGGGACTACA ATAAACCCCT 500 TTTTGTGGCA CCAGCCATGA ATACATTGAT GTGGAATAAT CCATTCACAG AACGACACCT 16350 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTGTGGCA CCAGCCATGA ATACATTGAT GTGGAATAAT CCATTCACAG AACGACACCT 560 TATGGTAATT GATGAGCTTG GAATCTCTCT CATACCACCA GTTTCAAAAA GACTAGCATG 16410 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATGGTAATT GATGAGCTTG GAATCTCTCT CATACCACCA GTTTCAAAAA GACTAGCATG 620 TGGAGATTAT GGAAATGGCG CTATGGCTGA ACCTTCTCTC ATCTACTCAA CTGTAAGACT 16470 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGAGATTAT GGAAATGGCG CTATGGCTGA ACCTTCTCTC ATCTACTCAA CTGTAAGACT 680 CTTCTATGAG TCACGGTCAC AATCAGGTGG CATCAACTTG GCTTGATTCG CAGATCATTA 16530 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCTATGAG TCACGGTCAC AATCAGGTGG CATCAACTTG GCTTGATTCG CAGATCATTA 740 AATTTTATTC ATCGGTTTGT ACAAGTGGTA GAAATTGTTG AAATTCAGGA CCTGGA 16586 |||||||||| |||||||||| |||||||||| |||||||||| ||| |||||| |||||| AATTTTATTC ATCGGTTTGT ACAAGTGGTA GAAATTGTTG AAA-TCAGGA CCTGGA 795 hqPGS_C08HBa0251H04.1+_SGN-E349881+ (12848 12922,15597 15943,16223 16586) ******************************************************************************** EST sequence 127 -strand 613 n (File: SGN-E273340-) 1 AATCAAGTGA GCTAACCACT CTGTGTTTCA ACTTCTGGTT ATAAATTTTT TAAAGCCTTT 61 GCTCCGTTAG ATTGCAGGTG GACTATGTGA TAACTTGTTA ACCTGCATCG TACGAGCATG 121 GGACTACAAT AAACCCCTTT TTGTGGCACC AGCCATGAAT ACATTGATGT GGAATAATCC 181 ATTCACAGAA CGACCCCTTA TGGTAATTGA TGAGCTTGGA ATCTCTCTCA TACCCCCAGT 241 TTCAAAAAGA CTAGCATGTG GAGATTATGG AAATGGCGCT ATGGCTGAAC CTTCTCTCAT 301 TTACTCAACT GTAAGACTCT TTTATGAGTC ACGGTCACAA TCAGGTGGCA TCAACTTGGC 361 TTGATTCGCA GATCATTAAA TTTTATTCAT CGGTTTGTAC AAGTGGTAAA AATTGTTGAA 421 ATTCAGGACC TGGAACAGTG TTACTTAGCA TACACCAGTT GTGTTTATTT TTCTCCTAAA 481 TTCTTCACCT CTTTAACTTG TGTCATGTTT GTATCCAAGC GGTAACTTTC AATCAAGTTT 541 CATGTTCATT GTAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAACC AAAAAAACTC 601 GAGACTAGTT CTC Predicted gene structure (within gDNA segment 12243 to 17905): Exon 1 12853 12922 ( 70 n); cDNA 1 70 ( 70 n); score: 1.000 Intron 1 12923 16222 (3300 n); Pd: 0.942 (s: 1.00), Pa: 0.965 (s: 1.00) Exon 2 16223 16705 ( 483 n); cDNA 71 553 ( 483 n); score: 0.990 PPA cDNA 563 589 MATCH C08HBa0251H04.1+ SGN-E273340- 0.991 553 0.902 C PGS_C08HBa0251H04.1+_SGN-E273340- (12853 12922,16223 16705) Alignment (genomic DNA sequence = upper lines): AATCAAGTGA GCTAACCACT CTGTGTTTCA ACTTCTGGTT ATAAATTTTT TAAAGCCTTT 12912 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATCAAGTGA GCTAACCACT CTGTGTTTCA ACTTCTGGTT ATAAATTTTT TAAAGCCTTT 60 GCTCCGTTAG GTAAAGTTTC TGCTCAGTGT CTTACTCTTT ACTTTTTTAG CTTTTCAATT 12972 |||||||||| GCTCCGTTAG .......... .......... .......... .......... .......... 70 TTGCATTTTT TTTAATCTCG TGTAATTGCT TAGTTTAATT ATGCAATAGT GTGCCTCATG 13032 .......... .......... .......... .......... .......... .......... 70 CAGAATAAAT TTTTTCATGA TATTGTTGAC CTCCCAAAAT AATTCAGTTA TAAATGCTTT 13092 .......... .......... .......... .......... .......... .......... 70 GTTGTAGAGC TCATGTTTTT CGCATCCCAA AGCAAAATCT CAGTATTTTA AGGCAATTTT 13152 .......... .......... .......... .......... .......... .......... 70 CTTCAATTCT AAGACAAACC CAGTTCAATT CTGTGTTTAA TCTTCCTGTT TCATCTGTCT 13212 .......... .......... .......... .......... .......... .......... 70 CAAAGCCGAT CCGAACAGTT TTACCCAATT CTTTGGTTAC CTCTTAAGGG TTTTGAGTTG 13272 .......... .......... .......... .......... .......... .......... 70 TGGGGTTTAA GATCAGATCA TACCATGGCT AGTCAATCCA GCAATCCTTA ATCTGTTTAT 13332 .......... .......... .......... .......... .......... .......... 70 GATTTCACTG TCAAGGTTAG AACCTTTGCT TATTTTGATT TGGGTTTTTT TCTAAATTGT 13392 .......... .......... .......... .......... .......... .......... 70 TCAATTTTGC TTATTTTGAC TGATCGGTTT AGAAAGAATG TTGTTTTTGT TTGTTTTATT 13452 .......... .......... .......... .......... .......... .......... 70 AGTGTTTGGG GTTTGGAAAT TGAGTTATGT GGTGTGGTTT TTATCAACTT TTGGGTTAGT 13512 .......... .......... .......... .......... .......... .......... 70 GGGTGATTGA AATTTGGTTT ATATTGGTTA TGAATCAGTA ACATAAGCAA AGAATGTGAA 13572 .......... .......... .......... .......... .......... .......... 70 AGATCATTCA TTTTTTGTTT TCTTTTGTGT TAAAGGTTGC TCATTTATCT ATGCTTTTGT 13632 .......... .......... .......... .......... .......... .......... 70 TTAGTTTCTT GGTTACCTCA TGAGTTTGAG CCTTGGAAAC ACGGTAAGGC TGCGTACGAT 13692 .......... .......... .......... .......... .......... .......... 70 AGGCTCTTGT GGTACGGCCC TTCGCCCCTT CCCGAGCTGG AGCATAGTGC ATTGGCTTGC 13752 .......... .......... .......... .......... .......... .......... 70 CTTTTGTTTT TTGCAATTAG AGATTTGGGG CTTTAAGTTC TGAGGAGTTA TTTTCAACTT 13812 .......... .......... .......... .......... .......... .......... 70 ATTAGCCCTT GCTTTATTGG TTTGTATAGA TTTGGAATGA CAAGATAGTG TAAAATATGG 13872 .......... .......... .......... .......... .......... .......... 70 TCTTTTCTAT TCACTTCATC TTTTGAGGTA AAGAGAGATG CATCTGCTGT GTAGGTAGCT 13932 .......... .......... .......... .......... .......... .......... 70 AGGTTAACTT GTTACATGAA TTTGGTTGAT CTAGACAAGC ATCTGTATTG CAAACACTTT 13992 .......... .......... .......... .......... .......... .......... 70 CCAACCATTT AAGTGTTATA GTGCAAATGA TTTTGATTTA CCAGAGAGGG AAAAAAAAAA 14052 .......... .......... .......... .......... .......... .......... 70 AGATAAGCAG AGGGATGTGT GTGAGCTTGT GATATATGGT ACCAAATTGG TGCTTGAAGC 14112 .......... .......... .......... .......... .......... .......... 70 AAACTAAGGC TAGTTGCTGC ATTTATCATC TAAACTTGGG GCTTGTTTTG TTTCTTTGGA 14172 .......... .......... .......... .......... .......... .......... 70 CTTGCTTCAC AATCATAACT ATTTGGACTT AACTCACCCT AGGTGTGGCC AAATCAATGC 14232 .......... .......... .......... .......... .......... .......... 70 TTAGCTGAAT TTCTCAAAAC CAATTTTAGT CCTCATCTTC ACAGACATCA AAAACAGTTG 14292 .......... .......... .......... .......... .......... .......... 70 GCCACCAGCT AATTAGCTGA TGTAGATACA CAGATTTGAA CTTTGACTTG CTTCATGACA 14352 .......... .......... .......... .......... .......... .......... 70 TGTTCACCTG AACTTTTAGT TGGTCCTTAA TTCGGTTAAA TTGGTTTTGC TGGTCTTTCA 14412 .......... .......... .......... .......... .......... .......... 70 TTTTGCTGAA CTAGCTTTCA GCTCGAGTAA ACTGACCTCA ACTCGGCATA TCTTTTTTTT 14472 .......... .......... .......... .......... .......... .......... 70 TTTTGCCTAG CTTAAAATAA GCTACTTGTT CTTGTAGAGG ACTAGTTGTT GACAGAGTCA 14532 .......... .......... .......... .......... .......... .......... 70 TTGACCGTAA CAACCTTTCA TTAGATTTGA TGGAACTTTG TAGTTCTGCT TCCATCATGG 14592 .......... .......... .......... .......... .......... .......... 70 TAATTCTAGA TTTCTCACAT TTGAAGGTGA GTTTCAAACT TGTCTTGAGG CTCATGGATT 14652 .......... .......... .......... .......... .......... .......... 70 GGATCCAAGT ACCACCATCA GTTTCTTTCT CAAGTGTTGT GGGCTTGTCA AATTAGTCAA 14712 .......... .......... .......... .......... .......... .......... 70 TTTCTTTAAC TGCTATTTGG GATGAATTAC TTATTTCCAC CTTAAATCAA TTCAATATTG 14772 .......... .......... .......... .......... .......... .......... 70 AATATGAACA GTCAATAGAA ATACCTAATT GTTGATAAAT TAGGGTTTTT GTAAGAGAAA 14832 .......... .......... .......... .......... .......... .......... 70 ATAAGATGGG TAGTGAATTG TTGATAAAGT TGGAATATTT TGGTATTCCT ATAGACTGTT 14892 .......... .......... .......... .......... .......... .......... 70 CATATTCAAT ATTGAATTGA TTTTGAGGTG GAAATAGGTA ATTCATCCCA ACTAGTTGTT 14952 .......... .......... .......... .......... .......... .......... 70 AAAGAAATTG ATTAATTTGG CAAGCCCATA ACACTTGAGA AAGAAACTGA TGGTGGTTAC 15012 .......... .......... .......... .......... .......... .......... 70 TTGGATCCAA TCCATGAGCC TCAAGACAAG TTTGAAACTC ACCTTCAAAT GTGAGAAATC 15072 .......... .......... .......... .......... .......... .......... 70 CAGAATTACC ATGATGGAAG CAGAACTACA AAATTCCATC AAATCTAAGG AAGGTTGTTA 15132 .......... .......... .......... .......... .......... .......... 70 AGGTCAATGA ATCTGTCAAA ATCTAGTCCT CTACAAGAAC AAGTAGCTTA TTTTACCCTA 15192 .......... .......... .......... .......... .......... .......... 70 GGCAAAAAAA AAAAGATGCC GAGTTGAGGT CACTTTACTC GAGCTGAAGG CTAGTTCAGC 15252 .......... .......... .......... .......... .......... .......... 70 AGAATGAAAG ACCAGCAAAC CGAATTAAGG ACCAATTAAC CAGTTCAAGT GAACATGTCA 15312 .......... .......... .......... .......... .......... .......... 70 GTAAGCAAGT CAAAGTTCAA ATCCGTGTAT CTACATCAGC TAACCAGCTG GTGGCCAATT 15372 .......... .......... .......... .......... .......... .......... 70 TTTTTTGATG TCTATGAAGA TGAGGGCTAA ATGAGAAATT AAGATAAGCA TTGATTTGGT 15432 .......... .......... .......... .......... .......... .......... 70 CACACCTAGG GTGAGTTAAG TCCAAAGAGA TGATTGTGAA GCAAGTTACC TGAGGGCAGA 15492 .......... .......... .......... .......... .......... .......... 70 TCTCTTAATT ATTTACTGTT GTTGTTCCCC TTAGTTGCAC GCATGTACAA TTACTTCTAT 15552 .......... .......... .......... .......... .......... .......... 70 CTTTCTCTTA GTGCAGTTAA AGAATCCTTT TATATGTATC ACAGGTTAGG TAGATACGGA 15612 .......... .......... .......... .......... .......... .......... 70 TCCTATGACT TCAGAGATGG AACCAGTTCA GATTAATGGT GCACCTAGGA GACCTCGTAT 15672 .......... .......... .......... .......... .......... .......... 70 TCTGCTGGCA GCAAGTGGAA GTGTGGCTTC AATTAAGTTT GCTAATCTAT GTCGTTGTTT 15732 .......... .......... .......... .......... .......... .......... 70 TTCTGAATGG GCAGAAGTTA AAGCAGTTGC AACGAAACCT TCTCTTCATT TCATAGACAA 15792 .......... .......... .......... .......... .......... .......... 70 AGCTTCGCTT CCGGAAGATG TCATTCTTTA TACTGATGAG GAGGAATGGT CCACTTGGAA 15852 .......... .......... .......... .......... .......... .......... 70 GAAGATTGGT GATAGTGTGC TACACATTGA GCTCCGCAGA TGGGCTGATA TTATGGTTAT 15912 .......... .......... .......... .......... .......... .......... 70 TGCCCCTTTG TCAGCAAACA CACTTGGGAA GGTGATGCAT CATTCCATGT TTTATTACTG 15972 .......... .......... .......... .......... .......... .......... 70 AAATTTTATT TTATGATAAC TCTCATTCTG CCTCAAAAAC ACAATTTATT CCCTTATCCT 16032 .......... .......... .......... .......... .......... .......... 70 GTACTAATTG ATCTTTATGA AATGGACTAT CAACAATGTT AAACATACTA ATTGAACATG 16092 .......... .......... .......... .......... .......... .......... 70 TCCATGCTAG AAGATTAACG TTAAGAATGA CACCAATTCA ATTCCTTACA TCATTAAGAA 16152 .......... .......... .......... .......... .......... .......... 70 AAAGTTTGTA GTATTACTTA TATTGCGATC TACTTTGCAA TTAAGCTGAT ATATGTCTTA 16212 .......... .......... .......... .......... .......... .......... 70 TGTACTGTAG ATTGCAGGTG GACTATGTGA TAACTTGTTA ACCTGCATCG TACGAGCATG 16272 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ATTGCAGGTG GACTATGTGA TAACTTGTTA ACCTGCATCG TACGAGCATG 120 GGACTACAAT AAACCCCTTT TTGTGGCACC AGCCATGAAT ACATTGATGT GGAATAATCC 16332 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGACTACAAT AAACCCCTTT TTGTGGCACC AGCCATGAAT ACATTGATGT GGAATAATCC 180 ATTCACAGAA CGACACCTTA TGGTAATTGA TGAGCTTGGA ATCTCTCTCA TACCACCAGT 16392 |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| |||| ||||| ATTCACAGAA CGACCCCTTA TGGTAATTGA TGAGCTTGGA ATCTCTCTCA TACCCCCAGT 240 TTCAAAAAGA CTAGCATGTG GAGATTATGG AAATGGCGCT ATGGCTGAAC CTTCTCTCAT 16452 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCAAAAAGA CTAGCATGTG GAGATTATGG AAATGGCGCT ATGGCTGAAC CTTCTCTCAT 300 CTACTCAACT GTAAGACTCT TCTATGAGTC ACGGTCACAA TCAGGTGGCA TCAACTTGGC 16512 ||||||||| |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| TTACTCAACT GTAAGACTCT TTTATGAGTC ACGGTCACAA TCAGGTGGCA TCAACTTGGC 360 TTGATTCGCA GATCATTAAA TTTTATTCAT CGGTTTGTAC AAGTGGTAGA AATTGTTGAA 16572 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| | |||||||||| TTGATTCGCA GATCATTAAA TTTTATTCAT CGGTTTGTAC AAGTGGTAAA AATTGTTGAA 420 ATTCAGGACC TGGAACAGTG TTACTTAGCA TACACCAGTT GTGTTTATTT TTCTCCTAAA 16632 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTCAGGACC TGGAACAGTG TTACTTAGCA TACACCAGTT GTGTTTATTT TTCTCCTAAA 480 TTCTTCACCT CTTTAACTTG TGTCATGTTT GTATCCAAGC GGTAACTTTC AATCAAGTTT 16692 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCTTCACCT CTTTAACTTG TGTCATGTTT GTATCCAAGC GGTAACTTTC AATCAAGTTT 540 CATGTTCATT GTA 16705 |||||||||| ||| CATGTTCATT GTA 553 hqPGS_C08HBa0251H04.1+_SGN-E273340- (12853 12922,16223 16705) ******************************************************************************** EST sequence 54 +strand 339 n (File: SGN-E201524+) 1 CTTCTCATCC CAAAGCAAAA TCTCAGTATT TTAAGGCGAT TTTCTTCGAT TCTAAGACAA 61 ACCCAGTTCA ATTCTGTGTT TAATCTTCCT GTTTCGTCTT TCTCTAAGCC GATCCGAACA 121 GTTTTACCCA ATTCTTTGGT TACCTCTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 181 CATACCATGG CTAGTCAATC AAGCAACCCT CAATCTGTTT ATGATTTCAC TGTCAAGGAT 241 GCTAAGGGCA AGGACGTTGA TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT 301 GTTGCATCAC AGTGTGGCCT GACTAATTCG AACTACACT Predicted gene structure (within gDNA segment 12465 to 14968): Exon 1 13111 13347 ( 237 n); cDNA 2 237 ( 236 n); score: 0.958 MATCH C08HBa0251H04.1+ SGN-E201524+ 0.958 237 0.699 C PGS_C08HBa0251H04.1+_SGN-E201524+ (13111 13347) Alignment (genomic DNA sequence = upper lines): TTCGCATCCC AAAGCAAAAT CTCAGTATTT TAAGGCAATT TTCTTCAATT CTAAGACAAA 13170 ||| |||||| |||||||||| |||||||||| |||||| ||| |||||| ||| |||||||||| TTCTCATCCC AAAGCAAAAT CTCAGTATTT TAAGGCGATT TTCTTCGATT CTAAGACAAA 61 CCCAGTTCAA TTCTGTGTTT AATCTTCCTG TTTCATCTGT CTCAAAGCCG ATCCGAACAG 13230 |||||||||| |||||||||| |||||||||| |||| ||| | ||| |||||| |||||||||| CCCAGTTCAA TTCTGTGTTT AATCTTCCTG TTTCGTCTTT CTCTAAGCCG ATCCGAACAG 121 TTTTACCCAA TTCTTTGGTT ACCTCTTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 13290 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| TTTTACCCAA TTCTTTGGTT ACCTC-TAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 180 CATACCATGG CTAGTCAATC CAGCAATCCT TAATCTGTTT ATGATTTCAC TGTCAAG 13347 |||||||||| |||||||||| ||||| ||| ||||||||| |||||||||| ||||||| CATACCATGG CTAGTCAATC AAGCAACCCT CAATCTGTTT ATGATTTCAC TGTCAAG 237 hqPGS_C08HBa0251H04.1+_SGN-E201524+ (13111 13347) ******************************************************************************** EST sequence 59 +strand 576 n (File: SGN-E390994+) 1 CAAAATCTCA GTATTTTAAG GCGATTTTCT TCGATTCTAA GACAAACCCA GTTCAATTCT 61 GTGTTTAATC TTCCTGTTTC GTCTTTCTCT AAGCCGATCC GAACAGTTTT ACCCAATTCT 121 TTGGTTACCT CTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 181 CAATCAAGCA ACCCTCAATC TGTTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA 241 CGTTGATCTC AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG 301 TGGCCTGACT AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA 361 AGGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT 421 TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA 481 GGTTGACGTG AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG 541 TGGGTTCTTT GGAAATGGTA TCAAGTGGAA CTTCTC Predicted gene structure (within gDNA segment 12525 to 17478): Exon 1 13125 13347 ( 223 n); cDNA 1 223 ( 223 n); score: 0.948 MATCH C08HBa0251H04.1+ SGN-E390994+ 0.948 223 0.387 C PGS_C08HBa0251H04.1+_SGN-E390994+ (13125 13347) Alignment (genomic DNA sequence = upper lines): CAAAATCTCA GTATTTTAAG GCAATTTTCT TCAATTCTAA GACAAACCCA GTTCAATTCT 13184 |||||||||| |||||||||| || ||||||| || ||||||| |||||||||| |||||||||| CAAAATCTCA GTATTTTAAG GCGATTTTCT TCGATTCTAA GACAAACCCA GTTCAATTCT 60 GTGTTTAATC TTCCTGTTTC ATCTGTCTCA AAGCCGATCC GAACAGTTTT ACCCAATTCT 13244 |||||||||| |||||||||| ||| |||| |||||||||| |||||||||| |||||||||| GTGTTTAATC TTCCTGTTTC GTCTTTCTCT AAGCCGATCC GAACAGTTTT ACCCAATTCT 120 TTGGTTACCT CTTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG 13304 |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGGTTACCT C-TAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG 179 TCAATCCAGC AATCCTTAAT CTG-TTTATG ATTTCACTGT CAAG 13347 |||||| ||| || ||| ||| ||| |||||| |||||||||| |||| TCAATCAAGC AACCCTCAAT CTGTTTTATG ATTTCACTGT CAAG 223 hqPGS_C08HBa0251H04.1+_SGN-E390994+ (13125 13347) ******************************************************************************** EST sequence 52 +strand 429 n (File: SGN-E287624+) 1 AAAATCTCAG TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG 61 TGTTTAATCT TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT 121 TGGTTACCTC TAACGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC 181 AATCAAGCAA CCCTCAATCT GTTTTATGAT TTCACTGTCA AGGATGCTAA CGGCAAGGAC 241 GTTGATCTCA GTGTTCACAA GGGAAAAGTC CTTATCATTG TCAATGTTGC ATGACAGTGT 301 CGGCCTGACT AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT GCAAGGATCA 361 ATGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGATTGGT GGACAGGAGC CTGGGAACAT 421 TGAAGATAT Predicted gene structure (within gDNA segment 12526 to 16018): Exon 1 13126 13347 ( 222 n); cDNA 1 222 ( 222 n); score: 0.944 MATCH C08HBa0251H04.1+ SGN-E287624+ 0.944 222 0.517 C PGS_C08HBa0251H04.1+_SGN-E287624+ (13126 13347) Alignment (genomic DNA sequence = upper lines): AAAATCTCAG TATTTTAAGG CAATTTTCTT CAATTCTAAG ACAAACCCAG TTCAATTCTG 13185 |||||||||| |||||||||| | |||||||| | |||||||| |||||||||| |||||||||| AAAATCTCAG TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG 60 TGTTTAATCT TCCTGTTTCA TCTGTCTCAA AGCCGATCCG AACAGTTTTA CCCAATTCTT 13245 |||||||||| ||||||||| ||| |||| | |||||||||| |||||||||| |||||||||| TGTTTAATCT TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT 120 TGGTTACCTC TTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 13305 |||||||||| ||| ||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTACCTC -TAACGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 179 CAATCCAGCA ATCCTTAATC TG-TTTATGA TTTCACTGTC AAG 13347 ||||| |||| | ||| |||| || ||||||| |||||||||| ||| CAATCAAGCA ACCCTCAATC TGTTTTATGA TTTCACTGTC AAG 222 hqPGS_C08HBa0251H04.1+_SGN-E287624+ (13126 13347) ******************************************************************************** EST sequence 31 +strand 327 n (File: SGN-E335811+) 1 ATCTCAGAAT TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT 61 TTAATCTTCC TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 121 TTACCTCTAA GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT 181 CAAGCAACCC TCAATCTGTT TATGATTTCA CTGTCAAGGA TGCTAATGGC AAGGACGTTG 241 ATCTCAGTAT TTACAAAGGA AAAGTACTTA TCATTGTCAA TGTTGCATCA CAGTGTGGGC 301 TGACTAATTC GAACTACACT GACATGA Predicted gene structure (within gDNA segment 12268 to 15038): Exon 1 13129 13347 ( 219 n); cDNA 1 218 ( 218 n); score: 0.954 MATCH C08HBa0251H04.1+ SGN-E335811+ 0.954 219 0.670 C PGS_C08HBa0251H04.1+_SGN-E335811+ (13129 13347) Alignment (genomic DNA sequence = upper lines): ATCTCAGTAT TTTAAGGCAA TTTTCTTCAA TTCTAAGACA AACCCAGTTC AATTCTGTGT 13188 ||||||| || |||||||| | |||||||| | |||||||||| |||||||||| |||||||||| ATCTCAGAAT TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT 60 TTAATCTTCC TGTTTCATCT GTCTCAAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 13248 |||||||||| |||||| ||| |||| |||| |||||||||| |||||||||| |||||||||| TTAATCTTCC TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 120 TTACCTCTTA AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA 13308 ||||||| || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTACCTC-TA AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA 179 TCCAGCAATC CTTAATCTGT TTATGATTTC ACTGTCAAG 13347 || ||||| | || ||||||| |||||||||| ||||||||| TCAAGCAACC CTCAATCTGT TTATGATTTC ACTGTCAAG 218 hqPGS_C08HBa0251H04.1+_SGN-E335811+ (13129 13347) ******************************************************************************** EST sequence 25 +strand 411 n (File: SGN-E291487+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT T Predicted gene structure (within gDNA segment 12318 to 15928): Exon 1 13134 13347 ( 214 n); cDNA 1 213 ( 213 n); score: 0.958 MATCH C08HBa0251H04.1+ SGN-E291487+ 0.958 214 0.521 C PGS_C08HBa0251H04.1+_SGN-E291487+ (13134 13347) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCAATTTTC TTCAATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 13193 |||||||||| ||| |||||| ||| |||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CATCTGTCTC AAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 13253 |||||||||| | ||| |||| ||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTTAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCCAG 13313 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || TC-TAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCAAG 179 CAATCCTTAA TCTGTTTATG ATTTCACTGT CAAG 13347 ||| ||| || |||||||||| |||||||||| |||| CAACCCTCAA TCTGTTTATG ATTTCACTGT CAAG 213 hqPGS_C08HBa0251H04.1+_SGN-E291487+ (13134 13347) ******************************************************************************** EST sequence 29 +strand 395 n (File: SGN-E232753+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACA Predicted gene structure (within gDNA segment 12318 to 15768): Exon 1 13134 13347 ( 214 n); cDNA 1 213 ( 213 n); score: 0.958 MATCH C08HBa0251H04.1+ SGN-E232753+ 0.958 214 0.542 C PGS_C08HBa0251H04.1+_SGN-E232753+ (13134 13347) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCAATTTTC TTCAATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 13193 |||||||||| ||| |||||| ||| |||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CATCTGTCTC AAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 13253 |||||||||| | ||| |||| ||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTTAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCCAG 13313 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || TC-TAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCAAG 179 CAATCCTTAA TCTGTTTATG ATTTCACTGT CAAG 13347 ||| ||| || |||||||||| |||||||||| |||| CAACCCTCAA TCTGTTTATG ATTTCACTGT CAAG 213 hqPGS_C08HBa0251H04.1+_SGN-E232753+ (13134 13347) ******************************************************************************** EST sequence 33 +strand 609 n (File: SGN-E275539+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC 421 CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTTGACGTG 481 AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT 541 GGAGATGGTA TCAAGTGGAA CTTCTCCAAA TTCCTCATTG ACAAAGAAGG ACATGTCGTT 601 GATCGCTAC Predicted gene structure (within gDNA segment 12318 to 17908): Exon 1 13134 13347 ( 214 n); cDNA 1 213 ( 213 n); score: 0.958 MATCH C08HBa0251H04.1+ SGN-E275539+ 0.958 214 0.351 C PGS_C08HBa0251H04.1+_SGN-E275539+ (13134 13347) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCAATTTTC TTCAATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 13193 |||||||||| ||| |||||| ||| |||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CATCTGTCTC AAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 13253 |||||||||| | ||| |||| ||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTTAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCCAG 13313 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || TC-TAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCAAG 179 CAATCCTTAA TCTGTTTATG ATTTCACTGT CAAG 13347 ||| ||| || |||||||||| |||||||||| |||| CAACCCTCAA TCTGTTTATG ATTTCACTGT CAAG 213 hqPGS_C08HBa0251H04.1+_SGN-E275539+ (13134 13347) ******************************************************************************** EST sequence 41 +strand 582 n (File: SGN-E308395+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTGCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC 421 CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTTGACGTG 481 AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT 541 GGAGATGGTA TCAAGTGGAA CTTCTCCAAA TTCCTCATTG AC Predicted gene structure (within gDNA segment 12318 to 17638): Exon 1 13134 13347 ( 214 n); cDNA 1 213 ( 213 n); score: 0.953 MATCH C08HBa0251H04.1+ SGN-E308395+ 0.953 214 0.368 C PGS_C08HBa0251H04.1+_SGN-E308395+ (13134 13347) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCAATTTTC TTCAATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 13193 |||||||||| ||| |||||| ||| |||||| |||||||||| ||| |||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTGCAATTC TGTGTTTAAT 60 CTTCCTGTTT CATCTGTCTC AAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 13253 |||||||||| | ||| |||| ||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTTAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCCAG 13313 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || TC-TAAGGGT TTTGAGTTGT GGGGTTTAAG ATCAGATCAT ACCATGGCTA GTCAATCAAG 179 CAATCCTTAA TCTGTTTATG ATTTCACTGT CAAG 13347 ||| ||| || |||||||||| |||||||||| |||| CAACCCTCAA TCTGTTTATG ATTTCACTGT CAAG 213 hqPGS_C08HBa0251H04.1+_SGN-E308395+ (13134 13347) ******************************************************************************** EST sequence 7 +strand 508 n (File: SGN-E295010+) 1 TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 61 TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 121 TAAGGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC AATCAAGCAA 181 CCCTCAATCT GTTTATGATT TCACTGTCAA GGATGCTAAG GGCAAGGACG TTGATCTCAG 241 TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGTG GCCTGACTAA 301 TTCGAACTAC ACTGACATGA CGGAGTTATA CAAGAAGTAC AAGGATCAAG GTTTGGAGAT 361 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 421 GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG TTGACGTGAA 481 TGGTGATAAT GCTGCTCCAT TATATAGG Predicted gene structure (within gDNA segment 12338 to 16918): Exon 1 13136 13347 ( 212 n); cDNA 1 211 ( 211 n); score: 0.958 MATCH C08HBa0251H04.1+ SGN-E295010+ 0.958 212 0.417 C PGS_C08HBa0251H04.1+_SGN-E295010+ (13136 13347) Alignment (genomic DNA sequence = upper lines): TATTTTAAGG CAATTTTCTT CAATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 13195 |||||||||| | |||||||| | |||||||| |||||||||| |||||||||| |||||||||| TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 60 TCCTGTTTCA TCTGTCTCAA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 13255 ||||||||| ||| |||| | |||||||||| |||||||||| |||||||||| |||||||||| TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 120 TTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT CAATCCAGCA 13315 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| |||| -TAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT CAATCAAGCA 179 ATCCTTAATC TGTTTATGAT TTCACTGTCA AG 13347 | ||| |||| |||||||||| |||||||||| || ACCCTCAATC TGTTTATGAT TTCACTGTCA AG 211 hqPGS_C08HBa0251H04.1+_SGN-E295010+ (13136 13347) ******************************************************************************** EST sequence 10 +strand 638 n (File: SGN-E371609+) 1 TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 61 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 121 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 181 CTCAATCTGT TTATGATTTC ACTGTCAAGG ATGCTAAGGG CAAGGACGTT GATCTCAGTA 241 TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTGGTAT GTATTAGTTT 301 CGTAGTTATT TTATTCATTA TATGTTTGAA GCCTCTTCAC AATATTTTGG ATTTCAATAG 361 GAACTTTCGA TGAGAGAAAT GATCTGATCG CTAGTTTTTG CTCCTTGTAA TTAAGTGAAA 421 TTTTCATGTG TTGCTCTCGA TAGAATTGAC TCAAAGTAAA GAAGTATTAA ACTTGAACCT 481 TATGCTACAT CAGATAGGGC ATTCCTCCCC GTGATGAGAA TACTATGCAT TGTGATAATA 541 ACTGAATTTA CGAGATATTT AGTGAAGTTT TCAGGTGTTG TCCTCCATAG AATTGACTGG 601 AAGTAAAAAA GTATTAAAAT TGAACCTTAT CTTACACT Predicted gene structure (within gDNA segment 12358 to 18238): Exon 1 13139 13347 ( 209 n); cDNA 1 209 ( 209 n); score: 0.945 MATCH C08HBa0251H04.1+ SGN-E371609+ 0.945 209 0.328 C PGS_C08HBa0251H04.1+_SGN-E371609+ (13139 13347) Alignment (genomic DNA sequence = upper lines): TTTAAGGC-A ATTTTCTTCA ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 13197 |||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 60 CTGTTTCATC TGTCTCAAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTT 13257 ||||||| || | |||| ||| |||||||||| |||||||||| |||||||||| |||||||| | CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTC-T 119 AAGGGTTTTG AGTTGTGGGG TTTAAGATCA GATCATACCA TGGCTAGTCA ATCCAGCAAT 13317 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| ||||| AAGGGTTTTG AGTTGTGGGG TTTAAGATCA GATCATACCA TGGCTAGTCA ATCAAGCAAC 179 CCTTAATCTG TTTATGATTT CACTGTCAAG 13347 ||| |||||| |||||||||| |||||||||| CCTCAATCTG TTTATGATTT CACTGTCAAG 209 hqPGS_C08HBa0251H04.1+_SGN-E371609+ (13139 13347) ******************************************************************************** EST sequence 16 +strand 529 n (File: SGN-E280323+) 1 TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 61 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 121 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 181 CTCAATCTGT TTATGATTTC ACTGTCAAGG ATGCTAAGGG CAAGGACGTT GATCTCAGTA 241 TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTGGTAT GTATTAGTTT 301 CGTAGTTATT TTATTCATTA TATGTTTGAA GCCTCTTCAC AATATTTTGG ATTTCAATAG 361 GAACTTTCGA TGAGAGAAAT GATCTGATCG CTAGTTTTTG CTCCTTGTAA TTAAGTGAAA 421 TTTTCATGTG TTGCTCTCGA TAGAATTGAC TCAAAGTAAA GAAGTATTAA ACTTGAACCT 481 TATGCTACAT CAGATAGGGC ATTCCTCCCC GTGATGAGAA TACTATGCA Predicted gene structure (within gDNA segment 12358 to 17148): Exon 1 13139 13347 ( 209 n); cDNA 1 209 ( 209 n); score: 0.945 MATCH C08HBa0251H04.1+ SGN-E280323+ 0.945 209 0.395 C PGS_C08HBa0251H04.1+_SGN-E280323+ (13139 13347) Alignment (genomic DNA sequence = upper lines): TTTAAGGC-A ATTTTCTTCA ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 13197 |||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 60 CTGTTTCATC TGTCTCAAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTT 13257 ||||||| || | |||| ||| |||||||||| |||||||||| |||||||||| |||||||| | CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTC-T 119 AAGGGTTTTG AGTTGTGGGG TTTAAGATCA GATCATACCA TGGCTAGTCA ATCCAGCAAT 13317 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| ||||| AAGGGTTTTG AGTTGTGGGG TTTAAGATCA GATCATACCA TGGCTAGTCA ATCAAGCAAC 179 CCTTAATCTG TTTATGATTT CACTGTCAAG 13347 ||| |||||| |||||||||| |||||||||| CCTCAATCTG TTTATGATTT CACTGTCAAG 209 hqPGS_C08HBa0251H04.1+_SGN-E280323+ (13139 13347) ******************************************************************************** EST sequence 46 +strand 284 n (File: SGN-E290421+) 1 TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 61 TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTCTAA 121 GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 181 TCAATCTGTT TATGATTTCA CTGTCAAGGA TGCTAAGGGC AAGGACGTTG ATCTCAGTAT 241 TTACAAGGGA AAAGTCCTTA TCATTGTCAA TGTTGCATCA CAGT Predicted gene structure (within gDNA segment 12368 to 14708): Exon 1 13139 13347 ( 209 n); cDNA 1 208 ( 208 n); score: 0.957 MATCH C08HBa0251H04.1+ SGN-E290421+ 0.957 209 0.736 C PGS_C08HBa0251H04.1+_SGN-E290421+ (13139 13347) Alignment (genomic DNA sequence = upper lines): TTTAAGGCAA TTTTCTTCAA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 13198 |||||||| | |||||||| | |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 60 TGTTTCATCT GTCTCAAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTCTTA 13258 |||||| ||| |||| |||| |||||||||| |||||||||| |||||||||| ||||||| || TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTC-TA 119 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCCAGCAATC 13318 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || ||||| | AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 179 CTTAATCTGT TTATGATTTC ACTGTCAAG 13347 || ||||||| |||||||||| ||||||||| CTCAATCTGT TTATGATTTC ACTGTCAAG 208 hqPGS_C08HBa0251H04.1+_SGN-E290421+ (13139 13347) ******************************************************************************** EST sequence 44 +strand 558 n (File: SGN-E290457+) 1 TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT 541 GGTATCAAGT GGAACTTC Predicted gene structure (within gDNA segment 12378 to 17458): Exon 1 13140 13347 ( 208 n); cDNA 1 207 ( 207 n); score: 0.957 MATCH C08HBa0251H04.1+ SGN-E290457+ 0.957 208 0.373 C PGS_C08HBa0251H04.1+_SGN-E290457+ (13140 13347) Alignment (genomic DNA sequence = upper lines): TTAAGGCAAT TTTCTTCAAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 13199 ||||||| || ||||||| || |||||||||| |||||||||| |||||||||| |||||||||| TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 60 GTTTCATCTG TCTCAAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTTAA 13259 ||||| ||| |||| ||||| |||||||||| |||||||||| |||||||||| |||||| ||| GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTC-TAA 119 GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CCAGCAATCC 13319 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | ||||| || GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 179 TTAATCTGTT TATGATTTCA CTGTCAAG 13347 | |||||||| |||||||||| |||||||| TCAATCTGTT TATGATTTCA CTGTCAAG 207 hqPGS_C08HBa0251H04.1+_SGN-E290457+ (13140 13347) ******************************************************************************** EST sequence 56 +strand 501 n (File: SGN-E283296+) 1 TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA T Predicted gene structure (within gDNA segment 12378 to 17488): Exon 1 13140 13347 ( 208 n); cDNA 1 207 ( 207 n); score: 0.957 MATCH C08HBa0251H04.1+ SGN-E283296+ 0.957 208 0.415 C PGS_C08HBa0251H04.1+_SGN-E283296+ (13140 13347) Alignment (genomic DNA sequence = upper lines): TTAAGGCAAT TTTCTTCAAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 13199 ||||||| || ||||||| || |||||||||| |||||||||| |||||||||| |||||||||| TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 60 GTTTCATCTG TCTCAAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTTAA 13259 ||||| ||| |||| ||||| |||||||||| |||||||||| |||||||||| |||||| ||| GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTC-TAA 119 GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CCAGCAATCC 13319 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | ||||| || GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 179 TTAATCTGTT TATGATTTCA CTGTCAAG 13347 | |||||||| |||||||||| |||||||| TCAATCTGTT TATGATTTCA CTGTCAAG 207 hqPGS_C08HBa0251H04.1+_SGN-E283296+ (13140 13347) ******************************************************************************** EST sequence 20 +strand 717 n (File: SGN-E390038+) 1 TACGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 61 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 121 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 181 AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT CTCAGTATTT 241 ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG ACTAATTCGA 301 ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG GAGATTCTGG 361 CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA 421 TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC GTGAATGGTG 481 ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC TTTGGAGATG 541 GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC GTTGATCGCT 601 ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC TTGGGTGTTG 661 CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT AGAAATA Predicted gene structure (within gDNA segment 12388 to 19058): Exon 1 13141 13347 ( 207 n); cDNA 1 206 ( 206 n); score: 0.952 MATCH C08HBa0251H04.1+ SGN-E390038+ 0.952 207 0.289 C PGS_C08HBa0251H04.1+_SGN-E390038+ (13141 13347) Alignment (genomic DNA sequence = upper lines): TAAGGCAATT TTCTTCAATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 13200 || ||| ||| |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| TACGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 60 TTTCATCTGT CTCAAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTTAAG 13260 |||| ||| | ||| |||||| |||||||||| |||||||||| |||||||||| ||||| |||| TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTC-TAAG 119 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC CAGCAATCCT 13320 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| ||| GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 179 TAATCTGTTT ATGATTTCAC TGTCAAG 13347 ||||||||| |||||||||| ||||||| CAATCTGTTT ATGATTTCAC TGTCAAG 206 hqPGS_C08HBa0251H04.1+_SGN-E390038+ (13141 13347) ******************************************************************************** EST sequence 23 +strand 710 n (File: SGN-E549946+) 1 TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 61 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 121 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 181 AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT CTCAGTATTT 241 ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG ACTAATTCGA 301 ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG GAGATTCTGG 361 CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA 421 TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC GTGAATGGTG 481 ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC TTTGGAGATG 541 GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC GTTGATCGCT 601 ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC TTGGGTGTTG 661 CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT Predicted gene structure (within gDNA segment 12388 to 18988): Exon 1 13141 13347 ( 207 n); cDNA 1 206 ( 206 n); score: 0.957 MATCH C08HBa0251H04.1+ SGN-E549946+ 0.957 207 0.292 C PGS_C08HBa0251H04.1+_SGN-E549946+ (13141 13347) Alignment (genomic DNA sequence = upper lines): TAAGGCAATT TTCTTCAATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 13200 |||||| ||| |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 60 TTTCATCTGT CTCAAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTTAAG 13260 |||| ||| | ||| |||||| |||||||||| |||||||||| |||||||||| ||||| |||| TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTC-TAAG 119 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC CAGCAATCCT 13320 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| ||| GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 179 TAATCTGTTT ATGATTTCAC TGTCAAG 13347 ||||||||| |||||||||| ||||||| CAATCTGTTT ATGATTTCAC TGTCAAG 206 hqPGS_C08HBa0251H04.1+_SGN-E549946+ (13141 13347) ******************************************************************************** EST sequence 36 +strand 647 n (File: SGN-E276053+) 1 AGAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT 541 GGTATCAAGT GGAACTTCTC CAAATTCCTC ATTGACAAAG AAGGACATGT CGTTGATCGC 601 TACTCTCCAA CCACTTCTCC AGCTAGCATG GAGAAGGATA TCAAGAA Predicted gene structure (within gDNA segment 12378 to 18348): Exon 1 13142 13347 ( 206 n); cDNA 3 207 ( 205 n); score: 0.956 MATCH C08HBa0251H04.1+ SGN-E276053+ 0.956 206 0.318 C PGS_C08HBa0251H04.1+_SGN-E276053+ (13142 13347) Alignment (genomic DNA sequence = upper lines): AAGGCAATTT TCTTCAATTC TAAGACAAAC CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT 13201 ||||| |||| ||||| |||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGCGATTT TCTTCGATTC TAAGACAAAC CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT 62 TTCATCTGTC TCAAAGCCGA TCCGAACAGT TTTACCCAAT TCTTTGGTTA CCTCTTAAGG 13261 ||| ||| || || ||||||| |||||||||| |||||||||| |||||||||| |||| ||||| TTCGTCTTTC TCTAAGCCGA TCCGAACAGT TTTACCCAAT TCTTTGGTTA CCTC-TAAGG 121 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCC AGCAATCCTT 13321 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ||||| ||| GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 181 AATCTGTTTA TGATTTCACT GTCAAG 13347 |||||||||| |||||||||| |||||| AATCTGTTTA TGATTTCACT GTCAAG 207 hqPGS_C08HBa0251H04.1+_SGN-E276053+ (13142 13347) ******************************************************************************** EST sequence 12 +strand 508 n (File: SGN-E239726+) 1 CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCGTCTTTC TCTAAGCCGA TCCGAACAGT 61 TTTACCCAAT TCTTTGGTTA CCTCTAAGGG TTTTGAGTTG TGGGGTTTAA GATCAGATCA 121 TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG TCAAGGATGC 181 TAAGGGCAAG GACGTTGATC TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT 241 TGCATCACAG TGTGGGCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 301 GTACAAGGAT CAAGGTTTGG AGATTCTGGC CTTCCCTTGC AACCAGTTTG GTGGACAGGA 361 GCCTGGAAAC ATTGAAGATA TCCAGCAAAT GGTCTGCACT CGCTTCAAGG CTGAGTACCC 421 CATATTTGAC AAGGTTGACG TGAATGGTGA TAATGCTGCT CCATTATATA GGTTTCTGAA 481 ATCAAGCAAA GGTGGGTTCT TTGGAGAT Predicted gene structure (within gDNA segment 12572 to 17278): Exon 1 13172 13347 ( 176 n); cDNA 1 175 ( 175 n); score: 0.960 MATCH C08HBa0251H04.1+ SGN-E239726+ 0.960 176 0.346 C PGS_C08HBa0251H04.1+_SGN-E239726+ (13172 13347) Alignment (genomic DNA sequence = upper lines): CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCATCTGTC TCAAAGCCGA TCCGAACAGT 13231 |||||||||| |||||||||| |||||||||| ||| ||| || || ||||||| |||||||||| CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCGTCTTTC TCTAAGCCGA TCCGAACAGT 60 TTTACCCAAT TCTTTGGTTA CCTCTTAAGG GTTTTGAGTT GTGGGGTTTA AGATCAGATC 13291 |||||||||| |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| TTTACCCAAT TCTTTGGTTA CCTC-TAAGG GTTTTGAGTT GTGGGGTTTA AGATCAGATC 119 ATACCATGGC TAGTCAATCC AGCAATCCTT AATCTGTTTA TGATTTCACT GTCAAG 13347 |||||||||| ||||||||| ||||| ||| |||||||||| |||||||||| |||||| ATACCATGGC TAGTCAATCA AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAG 175 hqPGS_C08HBa0251H04.1+_SGN-E239726+ (13172 13347) ******************************************************************************** EST sequence 84 -strand 636 n (File: SGN-E302606-) 1 CTGATGAGGA GGAATGGTCC ACTTGGTGCA AATTGGTAAT AGTGTGCTAC ACATTGAGCT 61 CCGCAGATGG GCTGATATTA TGGTTATTGC CCCTTTGTCA GCAAACACAC TTGGGAAGAT 121 TGCAGGTGGA CTATGTGATA ACTTGTTAAC CTGCATCGTA CGAGCATGGG ACTACAATAA 181 ACCCCTTTTT GTGGCCCCAG CCATGAATAC ATTGATGTGG AATAATCCAT TCACAGAACG 241 ACCCCTTATG GTAATTGATG AGCTTGGAAT CTCTCTCATA CCCCCAGTTT CAAAAAGACT 301 AGCATGTGGA GATTATGGAA ATGGCGCTAT GGCTGAACCT TTTTTCATTT ACTCAACTGT 361 AAGACTTTTT TATGAGTCAC GGTCACAATC AGGTGGCATC AACTTGGCTT GATTCGCAGA 421 TCATTAAATT TTATTCATCG GTTTGTACAA GTGGTAGAAA TTGTTGAAAT TCAGGACCTG 481 GAACAGTGTT ACTTAGCATA CACCAGTTGT GTTTATTTTT CTCCTAAATT CTTCCCCTCT 541 TTAACTTGTG TCATGTTTGT ATCCAAGCGG TAACTTTCAA TCAAGTTTCA TGTTCATTGT 601 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAG AAAAAA Predicted gene structure (within gDNA segment 15215 to 17655): Exon 1 15825 15943 ( 119 n); cDNA 1 118 ( 118 n); score: 0.958 Intron 1 15944 16222 ( 279 n); Pd: 0.999 (s: 1.00), Pa: 0.965 (s: 1.00) Exon 2 16223 16705 ( 483 n); cDNA 119 601 ( 483 n); score: 0.981 PPA cDNA 602 636 MATCH C08HBa0251H04.1+ SGN-E302606- 0.977 602 0.947 C PGS_C08HBa0251H04.1+_SGN-E302606- (15825 15943,16223 16705) Alignment (genomic DNA sequence = upper lines): CTGATGAGGA GGAATGGTCC ACTTGGAAGA AGATTGGTGA TAGTGTGCTA CACATTGAGC 15884 |||||||||| |||||||||| |||||| | | |||||| | |||||||||| |||||||||| CTGATGAGGA GGAATGGTCC ACTTGG-TGC AAATTGGTAA TAGTGTGCTA CACATTGAGC 59 TCCGCAGATG GGCTGATATT ATGGTTATTG CCCCTTTGTC AGCAAACACA CTTGGGAAGG 15944 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TCCGCAGATG GGCTGATATT ATGGTTATTG CCCCTTTGTC AGCAAACACA CTTGGGAAG. 118 TGATGCATCA TTCCATGTTT TATTACTGAA ATTTTATTTT ATGATAACTC TCATTCTGCC 16004 .......... .......... .......... .......... .......... .......... 118 TCAAAAACAC AATTTATTCC CTTATCCTGT ACTAATTGAT CTTTATGAAA TGGACTATCA 16064 .......... .......... .......... .......... .......... .......... 118 ACAATGTTAA ACATACTAAT TGAACATGTC CATGCTAGAA GATTAACGTT AAGAATGACA 16124 .......... .......... .......... .......... .......... .......... 118 CCAATTCAAT TCCTTACATC ATTAAGAAAA AGTTTGTAGT ATTACTTATA TTGCGATCTA 16184 .......... .......... .......... .......... .......... .......... 118 CTTTGCAATT AAGCTGATAT ATGTCTTATG TACTGTAGAT TGCAGGTGGA CTATGTGATA 16244 || |||||||||| |||||||||| .......... .......... .......... ........AT TGCAGGTGGA CTATGTGATA 140 ACTTGTTAAC CTGCATCGTA CGAGCATGGG ACTACAATAA ACCCCTTTTT GTGGCACCAG 16304 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| |||| ACTTGTTAAC CTGCATCGTA CGAGCATGGG ACTACAATAA ACCCCTTTTT GTGGCCCCAG 200 CCATGAATAC ATTGATGTGG AATAATCCAT TCACAGAACG ACACCTTATG GTAATTGATG 16364 |||||||||| |||||||||| |||||||||| |||||||||| || ||||||| |||||||||| CCATGAATAC ATTGATGTGG AATAATCCAT TCACAGAACG ACCCCTTATG GTAATTGATG 260 AGCTTGGAAT CTCTCTCATA CCACCAGTTT CAAAAAGACT AGCATGTGGA GATTATGGAA 16424 |||||||||| |||||||||| || ||||||| |||||||||| |||||||||| |||||||||| AGCTTGGAAT CTCTCTCATA CCCCCAGTTT CAAAAAGACT AGCATGTGGA GATTATGGAA 320 ATGGCGCTAT GGCTGAACCT TCTCTCATCT ACTCAACTGT AAGACTCTTC TATGAGTCAC 16484 |||||||||| |||||||||| | | |||| | |||||||||| |||||| || |||||||||| ATGGCGCTAT GGCTGAACCT TTTTTCATTT ACTCAACTGT AAGACTTTTT TATGAGTCAC 380 GGTCACAATC AGGTGGCATC AACTTGGCTT GATTCGCAGA TCATTAAATT TTATTCATCG 16544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTCACAATC AGGTGGCATC AACTTGGCTT GATTCGCAGA TCATTAAATT TTATTCATCG 440 GTTTGTACAA GTGGTAGAAA TTGTTGAAAT TCAGGACCTG GAACAGTGTT ACTTAGCATA 16604 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTGTACAA GTGGTAGAAA TTGTTGAAAT TCAGGACCTG GAACAGTGTT ACTTAGCATA 500 CACCAGTTGT GTTTATTTTT CTCCTAAATT CTTCACCTCT TTAACTTGTG TCATGTTTGT 16664 |||||||||| |||||||||| |||||||||| |||| ||||| |||||||||| |||||||||| CACCAGTTGT GTTTATTTTT CTCCTAAATT CTTCCCCTCT TTAACTTGTG TCATGTTTGT 560 ATCCAAGCGG TAACTTTCAA TCAAGTTTCA TGTTCATTGT A 16705 |||||||||| |||||||||| |||||||||| |||||||||| | ATCCAAGCGG TAACTTTCAA TCAAGTTTCA TGTTCATTGT A 601 hqPGS_C08HBa0251H04.1+_SGN-E302606- (15825 15943,16223 16705) ******************************************************************************** EST sequence 53 +strand 339 n (File: SGN-E201524+) 1 CTTCTCATCC CAAAGCAAAA TCTCAGTATT TTAAGGCGAT TTTCTTCGAT TCTAAGACAA 61 ACCCAGTTCA ATTCTGTGTT TAATCTTCCT GTTTCGTCTT TCTCTAAGCC GATCCGAACA 121 GTTTTACCCA ATTCTTTGGT TACCTCTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 181 CATACCATGG CTAGTCAATC AAGCAACCCT CAATCTGTTT ATGATTTCAC TGTCAAGGAT 241 GCTAAGGGCA AGGACGTTGA TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT 301 GTTGCATCAC AGTGTGGCCT GACTAATTCG AACTACACT Predicted gene structure (within gDNA segment 20695 to 23436): Exon 1 21295 21531 ( 237 n); cDNA 1 237 ( 237 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 238 314 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 0) Exon 3 23258 23282 ( 25 n); cDNA 315 339 ( 25 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E201524+ 1.000 339 1.000 C PGS_C08HBa0251H04.1+_SGN-E201524+ (21295 21531,22500 22576,23258 23282) Alignment (genomic DNA sequence = upper lines): CTTCTCATCC CAAAGCAAAA TCTCAGTATT TTAAGGCGAT TTTCTTCGAT TCTAAGACAA 21354 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCTCATCC CAAAGCAAAA TCTCAGTATT TTAAGGCGAT TTTCTTCGAT TCTAAGACAA 60 ACCCAGTTCA ATTCTGTGTT TAATCTTCCT GTTTCGTCTT TCTCTAAGCC GATCCGAACA 21414 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCCAGTTCA ATTCTGTGTT TAATCTTCCT GTTTCGTCTT TCTCTAAGCC GATCCGAACA 120 GTTTTACCCA ATTCTTTGGT TACCTCTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 21474 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTACCCA ATTCTTTGGT TACCTCTAAG GGTTTTGAGT TGTGGGGTTT AAGATCAGAT 180 CATACCATGG CTAGTCAATC AAGCAACCCT CAATCTGTTT ATGATTTCAC TGTCAAGGTT 21534 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| CATACCATGG CTAGTCAATC AAGCAACCCT CAATCTGTTT ATGATTTCAC TGTCAAG... 237 AGGACTTTTG CTTATTTTGA TTTGGGGTTT TCCTGAAATG TTCAATTTTG CTTATTTTGA 21594 .......... .......... .......... .......... .......... .......... 237 CTGATTGGTT TAGAAAGAAT GTTGTTTTTG TTTGTTTTGT TAGTGTTTGG GGTTAGGAAG 21654 .......... .......... .......... .......... .......... .......... 237 TTGAGTTATG TGGTGTGGTT TTTATCATTT TTTGGGTTCG TGGGCGGTTA AAATTTGGTT 21714 .......... .......... .......... .......... .......... .......... 237 TTATATTGGG TATGAATCAG TAACATAAGC AATGAATGTG AAAGATTATT TATTTTTGTT 21774 .......... .......... .......... .......... .......... .......... 237 TTCTTTTGTG TTAAAGGTTG CTCATTTATT AATGCTGTTG TTTAGTTTTT TTGGTTTATG 21834 .......... .......... .......... .......... .......... .......... 237 GTCGTACCTT ATGAGTGTGA GCCTTAGAAA CACGGTAAGG CTGCTTGCAA TAGACTCTTG 21894 .......... .......... .......... .......... .......... .......... 237 TGGCTTGGCC CTTCCCTGAG CTGGAGCTTA GTGCATTGGC TTGCTTTTTT GTCTTTTGCA 21954 .......... .......... .......... .......... .......... .......... 237 ATTAGAGATT TGGGGCTTTA AGTTATGAGG AGTTGTTTTC AACTTATTAG CTCTTGCTTT 22014 .......... .......... .......... .......... .......... .......... 237 ATTGGTTTAT ATAGATTTGG AATGACAAGA TAAGCATACA GTGCGAAATT TCAGTCTTCT 22074 .......... .......... .......... .......... .......... .......... 237 TTGTTCACTT CTATCTTTAG GGTGTGAAGA GAGATGCCTC TGCTGTGTAG TTAGCTGGGT 22134 .......... .......... .......... .......... .......... .......... 237 TTAACTTGTT ACATGAATGT GGTTGATCTA CACAAGCATC TTGTATTGCA TACACTTTCC 22194 .......... .......... .......... .......... .......... .......... 237 AACCATCTAA GTGCTATTAG TATAGATGAT TTTGACTTAT CAGAAAGGAA AATAAAAGAT 22254 .......... .......... .......... .......... .......... .......... 237 GAGCAGAGGT GTGAGATTGT GATATATGGT ATAAACTTGG TGCTAGCAAG AAACTAAGGC 22314 .......... .......... .......... .......... .......... .......... 237 TTGTTGTTGC ATTATTATCT AAACTTGGGG CTTGTTTTTG TTTCTTTATT GTCAAAAAGT 22374 .......... .......... .......... .......... .......... .......... 237 TTTGTTTTAG GTCTGTTTTG ATATTTTCAA TATAGTATGT TGAGAGTAGT TCTTTTTTGG 22434 .......... .......... .......... .......... .......... .......... 237 GACTATACAG TTTGAATAAC CTGAATAAAA TCTTGGATAA TTGAAGTTCT TTTTATTCTT 22494 .......... .......... .......... .......... .......... .......... 237 TATAGGATGC TAAGGGCAAG GACGTTGATC TCAGTATTTA CAAGGGAAAA GTCCTTATCA 22554 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....GATGC TAAGGGCAAG GACGTTGATC TCAGTATTTA CAAGGGAAAA GTCCTTATCA 292 TTGTCAATGT TGCATCACAG TGGTATGTAT TAGTTTCGTA GTTATTTTAT TCATTATATG 22614 |||||||||| |||||||||| || TTGTCAATGT TGCATCACAG TG........ .......... .......... .......... 314 TTTGAAGCCT CTTCACAATA TTTTGGATTT CAATAGGAAC TTTCGATGAG AGAAATGATC 22674 .......... .......... .......... .......... .......... .......... 314 TGATCGCTAG TTTTTGCTCC TTGTAATTAA GTGAAATTTT CATGTGTTGC TCTCGATAGA 22734 .......... .......... .......... .......... .......... .......... 314 ATTGACTCAA AGTAAAGAAG TATTAAACTT GAACCTTATG CTACATCAGA TAGGGCATTC 22794 .......... .......... .......... .......... .......... .......... 314 CTCCCCGTGA TGAGAATACT ATGCATTGTG ATAATAACTG AATTTACGAG ATATTTAGTG 22854 .......... .......... .......... .......... .......... .......... 314 AAGTTTTCAG GTGTTGTCCT CCATAGAATT GACTGGAAGT AAAAAAGTAT TAAAATTGAA 22914 .......... .......... .......... .......... .......... .......... 314 CCTTATCTTA CACTGGATAG GGCGTTCCTC CTTGTGATGA AAATACTATG CATTTTTATA 22974 .......... .......... .......... .......... .......... .......... 314 AAAACTGAAT CTACGAGAAA TTGAACATGA CATTATTTTA CTCGAGCCCT GACTAAACAT 23034 .......... .......... .......... .......... .......... .......... 314 TCTTACTATG CTTGGTCCAC TACTCAACAA ACGTGTTCTT AGTACCACTA ATGACAAGTG 23094 .......... .......... .......... .......... .......... .......... 314 ATTGTGCATA TCCAGATAAG ATGTCTTTTT CATTTCGGTA TAATTTGATT TCATTGTGTT 23154 .......... .......... .......... .......... .......... .......... 314 TACTGTGAAA GGTTTGAGCA GTTACTTGTT GTATTTATGA TATCATTCTT TGATCTTGTT 23214 .......... .......... .......... .......... .......... .......... 314 ACATTTGCTT ATGACTGTTA ACTGATTCCA TTTTCTGATG CAGTGGCCTG ACTAATTCGA 23274 ||||||| |||||||||| .......... .......... .......... .......... ...TGGCCTG ACTAATTCGA 331 ACTACACT 23282 |||||||| ACTACACT 339 hqPGS_C08HBa0251H04.1+_SGN-E201524+ (21295 21531,22500 22576,23258 23282) ******************************************************************************** EST sequence 58 +strand 576 n (File: SGN-E390994+) 1 CAAAATCTCA GTATTTTAAG GCGATTTTCT TCGATTCTAA GACAAACCCA GTTCAATTCT 61 GTGTTTAATC TTCCTGTTTC GTCTTTCTCT AAGCCGATCC GAACAGTTTT ACCCAATTCT 121 TTGGTTACCT CTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 181 CAATCAAGCA ACCCTCAATC TGTTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA 241 CGTTGATCTC AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG 301 TGGCCTGACT AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA 361 AGGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT 421 TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA 481 GGTTGACGTG AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG 541 TGGGTTCTTT GGAAATGGTA TCAAGTGGAA CTTCTC Predicted gene structure (within gDNA segment 20710 to 24406): Exon 1 21310 21531 ( 222 n); cDNA 1 223 ( 223 n); score: 0.989 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0.95), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 224 300 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 301 362 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 363 481 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23796 ( 95 n); cDNA 482 576 ( 95 n); score: 0.989 MATCH C08HBa0251H04.1+ SGN-E390994+ 0.994 575 0.998 C PGS_C08HBa0251H04.1+_SGN-E390994+ (21310 21531,22500 22576,23258 23319,23469 23587,23702 23796) Alignment (genomic DNA sequence = upper lines): CAAAATCTCA GTATTTTAAG GCGATTTTCT TCGATTCTAA GACAAACCCA GTTCAATTCT 21369 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAAATCTCA GTATTTTAAG GCGATTTTCT TCGATTCTAA GACAAACCCA GTTCAATTCT 60 GTGTTTAATC TTCCTGTTTC GTCTTTCTCT AAGCCGATCC GAACAGTTTT ACCCAATTCT 21429 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGTTTAATC TTCCTGTTTC GTCTTTCTCT AAGCCGATCC GAACAGTTTT ACCCAATTCT 120 TTGGTTACCT CTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 21489 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGGTTACCT CTAAGGGTTT TGAGTTGTGG GGTTTAAGAT CAGATCATAC CATGGCTAGT 180 CAATCAAGCA ACCCTCAATC TG-TTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA 21548 |||||||||| |||||||||| || ||||||| |||||||||| ||| CAATCAAGCA ACCCTCAATC TGTTTTATGA TTTCACTGTC AAG....... .......... 223 TTTTGATTTG GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA 21608 .......... .......... .......... .......... .......... .......... 223 AAGAATGTTG TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT 21668 .......... .......... .......... .......... .......... .......... 223 GTGGTTTTTA TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG 21728 .......... .......... .......... .......... .......... .......... 223 AATCAGTAAC ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA 21788 .......... .......... .......... .......... .......... .......... 223 AGGTTGCTCA TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA 21848 .......... .......... .......... .......... .......... .......... 223 GTGTGAGCCT TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC 21908 .......... .......... .......... .......... .......... .......... 223 CCTGAGCTGG AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG 21968 .......... .......... .......... .......... .......... .......... 223 GCTTTAAGTT ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG 22028 .......... .......... .......... .......... .......... .......... 223 ATTTGGAATG ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT 22088 .......... .......... .......... .......... .......... .......... 223 CTTTAGGGTG TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT 22148 .......... .......... .......... .......... .......... .......... 223 GAATGTGGTT GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC 22208 .......... .......... .......... .......... .......... .......... 223 TATTAGTATA GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA 22268 .......... .......... .......... .......... .......... .......... 223 GATTGTGATA TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA 22328 .......... .......... .......... .......... .......... .......... 223 TTATCTAAAC TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT 22388 .......... .......... .......... .......... .......... .......... 223 GTTTTGATAT TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG 22448 .......... .......... .......... .......... .......... .......... 223 AATAACCTGA ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG 22508 ||||||||| .......... .......... .......... .......... .......... .GATGCTAAG 232 GGCAAGGACG TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA 22568 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCAAGGACG TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA 292 TCACAGTGGT ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC 22628 |||||||| TCACAGTG.. .......... .......... .......... .......... .......... 300 ACAATATTTT GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT 22688 .......... .......... .......... .......... .......... .......... 300 TGCTCCTTGT AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA 22748 .......... .......... .......... .......... .......... .......... 300 AAGAAGTATT AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG 22808 .......... .......... .......... .......... .......... .......... 300 AATACTATGC ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT 22868 .......... .......... .......... .......... .......... .......... 300 TGTCCTCCAT AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT 22928 .......... .......... .......... .......... .......... .......... 300 GGATAGGGCG TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC 22988 .......... .......... .......... .......... .......... .......... 300 GAGAAATTGA ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG 23048 .......... .......... .......... .......... .......... .......... 300 GTCCACTACT CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA 23108 .......... .......... .......... .......... .......... .......... 300 GATAAGATGT CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT 23168 .......... .......... .......... .......... .......... .......... 300 TGAGCAGTTA CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA 23228 .......... .......... .......... .......... .......... .......... 300 CTGTTAACTG ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG 23288 | |||||||||| |||||||||| |||||||||| .......... .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG 331 ACGGAGTTAT ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG 23348 |||||||||| |||||||||| |||||||||| | ACGGAGTTAT ACAAGAAGTA CAAGGATCAA G......... .......... .......... 362 CCCTCGATTC TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA 23408 .......... .......... .......... .......... .......... .......... 362 ATAAAATGGA TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG 23468 .......... .......... .......... .......... .......... .......... 362 GTTTGGAGAT TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG 23528 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTGGAGAT TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG 422 AAGATATCCA GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG 23588 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AAGATATCCA GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAG. 481 TAGAAATAAC ACGAAGCATT CTTCTCTTCT AGTCATCTAC ACTACAGCTG AAACTGAGAA 23648 .......... .......... .......... .......... .......... .......... 481 TAAATGAGGC AAGCTGTATG TACTGACATC TTCGTGTGTT TTGTGTGGTC TAGGTTGACG 23708 ||||||| .......... .......... .......... .......... .......... ...GTTGACG 488 TGAATGGTGA TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT 23768 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAATGGTGA TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT 548 TTGGAGATGG TATCAAGTGG AACTTCTC 23796 ||||| |||| |||||||||| |||||||| TTGGAAATGG TATCAAGTGG AACTTCTC 576 hqPGS_C08HBa0251H04.1+_SGN-E390994+ (21310 21531,22500 22576,23258 23319,23469 23587,23702 23796) ******************************************************************************** EST sequence 51 +strand 429 n (File: SGN-E287624+) 1 AAAATCTCAG TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG 61 TGTTTAATCT TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT 121 TGGTTACCTC TAACGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC 181 AATCAAGCAA CCCTCAATCT GTTTTATGAT TTCACTGTCA AGGATGCTAA CGGCAAGGAC 241 GTTGATCTCA GTGTTCACAA GGGAAAAGTC CTTATCATTG TCAATGTTGC ATGACAGTGT 301 CGGCCTGACT AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT GCAAGGATCA 361 ATGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGATTGGT GGACAGGAGC CTGGGAACAT 421 TGAAGATAT Predicted gene structure (within gDNA segment 20711 to 24280): Exon 1 21311 21531 ( 221 n); cDNA 1 222 ( 222 n); score: 0.984 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0.95), Pa: 0.999 (s: 0.94) Exon 2 22500 22576 ( 77 n); cDNA 223 299 ( 77 n); score: 0.948 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 0.94), Pa: 1.000 (s: 0.95) Exon 3 23258 23319 ( 62 n); cDNA 300 362 ( 63 n); score: 0.927 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 0.96), Pa: 1.000 (s: 0.98) Exon 4 23469 23535 ( 67 n); cDNA 363 429 ( 67 n); score: 0.970 MATCH C08HBa0251H04.1+ SGN-E287624+ 0.967 427 0.995 C PGS_C08HBa0251H04.1+_SGN-E287624+ (21311 21531,22500 22576,23258 23319,23469 23535) Alignment (genomic DNA sequence = upper lines): AAAATCTCAG TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG 21370 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAATCTCAG TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG 60 TGTTTAATCT TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT 21430 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTTAATCT TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT 120 TGGTTACCTC TAAGGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC 21490 |||||||||| ||| |||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTACCTC TAACGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC 180 AATCAAGCAA CCCTCAATCT G-TTTATGAT TTCACTGTCA AGGTTAGGAC TTTTGCTTAT 21549 |||||||||| |||||||||| | |||||||| |||||||||| || AATCAAGCAA CCCTCAATCT GTTTTATGAT TTCACTGTCA AG........ .......... 222 TTTGATTTGG GGTTTTCCTG AAATGTTCAA TTTTGCTTAT TTTGACTGAT TGGTTTAGAA 21609 .......... .......... .......... .......... .......... .......... 222 AGAATGTTGT TTTTGTTTGT TTTGTTAGTG TTTGGGGTTA GGAAGTTGAG TTATGTGGTG 21669 .......... .......... .......... .......... .......... .......... 222 TGGTTTTTAT CATTTTTTGG GTTCGTGGGC GGTTAAAATT TGGTTTTATA TTGGGTATGA 21729 .......... .......... .......... .......... .......... .......... 222 ATCAGTAACA TAAGCAATGA ATGTGAAAGA TTATTTATTT TTGTTTTCTT TTGTGTTAAA 21789 .......... .......... .......... .......... .......... .......... 222 GGTTGCTCAT TTATTAATGC TGTTGTTTAG TTTTTTTGGT TTATGGTCGT ACCTTATGAG 21849 .......... .......... .......... .......... .......... .......... 222 TGTGAGCCTT AGAAACACGG TAAGGCTGCT TGCAATAGAC TCTTGTGGCT TGGCCCTTCC 21909 .......... .......... .......... .......... .......... .......... 222 CTGAGCTGGA GCTTAGTGCA TTGGCTTGCT TTTTTGTCTT TTGCAATTAG AGATTTGGGG 21969 .......... .......... .......... .......... .......... .......... 222 CTTTAAGTTA TGAGGAGTTG TTTTCAACTT ATTAGCTCTT GCTTTATTGG TTTATATAGA 22029 .......... .......... .......... .......... .......... .......... 222 TTTGGAATGA CAAGATAAGC ATACAGTGCG AAATTTCAGT CTTCTTTGTT CACTTCTATC 22089 .......... .......... .......... .......... .......... .......... 222 TTTAGGGTGT GAAGAGAGAT GCCTCTGCTG TGTAGTTAGC TGGGTTTAAC TTGTTACATG 22149 .......... .......... .......... .......... .......... .......... 222 AATGTGGTTG ATCTACACAA GCATCTTGTA TTGCATACAC TTTCCAACCA TCTAAGTGCT 22209 .......... .......... .......... .......... .......... .......... 222 ATTAGTATAG ATGATTTTGA CTTATCAGAA AGGAAAATAA AAGATGAGCA GAGGTGTGAG 22269 .......... .......... .......... .......... .......... .......... 222 ATTGTGATAT ATGGTATAAA CTTGGTGCTA GCAAGAAACT AAGGCTTGTT GTTGCATTAT 22329 .......... .......... .......... .......... .......... .......... 222 TATCTAAACT TGGGGCTTGT TTTTGTTTCT TTATTGTCAA AAAGTTTTGT TTTAGGTCTG 22389 .......... .......... .......... .......... .......... .......... 222 TTTTGATATT TTCAATATAG TATGTTGAGA GTAGTTCTTT TTTGGGACTA TACAGTTTGA 22449 .......... .......... .......... .......... .......... .......... 222 ATAACCTGAA TAAAATCTTG GATAATTGAA GTTCTTTTTA TTCTTTATAG GATGCTAAGG 22509 |||||||| | .......... .......... .......... .......... .......... GATGCTAACG 232 GCAAGGACGT TGATCTCAGT ATTTACAAGG GAAAAGTCCT TATCATTGTC AATGTTGCAT 22569 |||||||||| |||||||||| || |||||| |||||||||| |||||||||| |||||||||| GCAAGGACGT TGATCTCAGT GTTCACAAGG GAAAAGTCCT TATCATTGTC AATGTTGCAT 292 CACAGTGGTA TGTATTAGTT TCGTAGTTAT TTTATTCATT ATATGTTTGA AGCCTCTTCA 22629 |||||| GACAGTG... .......... .......... .......... .......... .......... 299 CAATATTTTG GATTTCAATA GGAACTTTCG ATGAGAGAAA TGATCTGATC GCTAGTTTTT 22689 .......... .......... .......... .......... .......... .......... 299 GCTCCTTGTA ATTAAGTGAA ATTTTCATGT GTTGCTCTCG ATAGAATTGA CTCAAAGTAA 22749 .......... .......... .......... .......... .......... .......... 299 AGAAGTATTA AACTTGAACC TTATGCTACA TCAGATAGGG CATTCCTCCC CGTGATGAGA 22809 .......... .......... .......... .......... .......... .......... 299 ATACTATGCA TTGTGATAAT AACTGAATTT ACGAGATATT TAGTGAAGTT TTCAGGTGTT 22869 .......... .......... .......... .......... .......... .......... 299 GTCCTCCATA GAATTGACTG GAAGTAAAAA AGTATTAAAA TTGAACCTTA TCTTACACTG 22929 .......... .......... .......... .......... .......... .......... 299 GATAGGGCGT TCCTCCTTGT GATGAAAATA CTATGCATTT TTATAAAAAC TGAATCTACG 22989 .......... .......... .......... .......... .......... .......... 299 AGAAATTGAA CATGACATTA TTTTACTCGA GCCCTGACTA AACATTCTTA CTATGCTTGG 23049 .......... .......... .......... .......... .......... .......... 299 TCCACTACTC AACAAACGTG TTCTTAGTAC CACTAATGAC AAGTGATTGT GCATATCCAG 23109 .......... .......... .......... .......... .......... .......... 299 ATAAGATGTC TTTTTCATTT CGGTATAATT TGATTTCATT GTGTTTACTG TGAAAGGTTT 23169 .......... .......... .......... .......... .......... .......... 299 GAGCAGTTAC TTGTTGTATT TATGATATCA TTCTTTGATC TTGTTACATT TGCTTATGAC 23229 .......... .......... .......... .......... .......... .......... 299 TGTTAACTGA TTCCATTTTC TGATGCAGT- GGCCTGACTA ATTCGAACTA CACTGACATG 23288 | |||||||||| |||||||||| |||||||||| .......... .......... ........TC GGCCTGACTA ATTCGAACTA CACTGACATG 331 ACGGAGTTAT ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG 23348 |||||||||| ||||||||| |||||||||| ACGGAGTTAT ACAAGAAGTG CAAGGATCAA T......... .......... .......... 362 CCCTCGATTC TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA 23408 .......... .......... .......... .......... .......... .......... 362 ATAAAATGGA TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG 23468 .......... .......... .......... .......... .......... .......... 362 GTTTGGAGAT TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG 23528 |||||||||| |||||||||| |||||||||| || ||||||| |||||||||| || ||||||| GTTTGGAGAT TCTGGCCTTC CCTTGCAACC AGATTGGTGG ACAGGAGCCT GGGAACATTG 422 AAGATAT 23535 ||||||| AAGATAT 429 hqPGS_C08HBa0251H04.1+_SGN-E287624+ (21311 21531,22500 22576,23258 23319,23469 23535) ******************************************************************************** EST sequence 30 +strand 327 n (File: SGN-E335811+) 1 ATCTCAGAAT TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT 61 TTAATCTTCC TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 121 TTACCTCTAA GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT 181 CAAGCAACCC TCAATCTGTT TATGATTTCA CTGTCAAGGA TGCTAATGGC AAGGACGTTG 241 ATCTCAGTAT TTACAAAGGA AAAGTACTTA TCATTGTCAA TGTTGCATCA CAGTGTGGGC 301 TGACTAATTC GAACTACACT GACATGA Predicted gene structure (within gDNA segment 20642 to 23506): Exon 1 21314 21531 ( 218 n); cDNA 1 218 ( 218 n); score: 0.995 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 0.94) Exon 2 22500 22576 ( 77 n); cDNA 219 295 ( 77 n); score: 0.961 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 0.96), Pa: 1.000 (s: 0) Exon 3 23258 23289 ( 32 n); cDNA 296 327 ( 32 n); score: 0.969 MATCH C08HBa0251H04.1+ SGN-E335811+ 0.986 327 1.000 C PGS_C08HBa0251H04.1+_SGN-E335811+ (21314 21531,22500 22576,23258 23289) Alignment (genomic DNA sequence = upper lines): ATCTCAGTAT TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT 21373 ||||||| || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCTCAGAAT TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT 60 TTAATCTTCC TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 21433 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAATCTTCC TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG 120 TTACCTCTAA GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT 21493 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTACCTCTAA GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT 180 CAAGCAACCC TCAATCTGTT TATGATTTCA CTGTCAAGGT TAGGACTTTT GCTTATTTTG 21553 |||||||||| |||||||||| |||||||||| |||||||| CAAGCAACCC TCAATCTGTT TATGATTTCA CTGTCAAG.. .......... .......... 218 ATTTGGGGTT TTCCTGAAAT GTTCAATTTT GCTTATTTTG ACTGATTGGT TTAGAAAGAA 21613 .......... .......... .......... .......... .......... .......... 218 TGTTGTTTTT GTTTGTTTTG TTAGTGTTTG GGGTTAGGAA GTTGAGTTAT GTGGTGTGGT 21673 .......... .......... .......... .......... .......... .......... 218 TTTTATCATT TTTTGGGTTC GTGGGCGGTT AAAATTTGGT TTTATATTGG GTATGAATCA 21733 .......... .......... .......... .......... .......... .......... 218 GTAACATAAG CAATGAATGT GAAAGATTAT TTATTTTTGT TTTCTTTTGT GTTAAAGGTT 21793 .......... .......... .......... .......... .......... .......... 218 GCTCATTTAT TAATGCTGTT GTTTAGTTTT TTTGGTTTAT GGTCGTACCT TATGAGTGTG 21853 .......... .......... .......... .......... .......... .......... 218 AGCCTTAGAA ACACGGTAAG GCTGCTTGCA ATAGACTCTT GTGGCTTGGC CCTTCCCTGA 21913 .......... .......... .......... .......... .......... .......... 218 GCTGGAGCTT AGTGCATTGG CTTGCTTTTT TGTCTTTTGC AATTAGAGAT TTGGGGCTTT 21973 .......... .......... .......... .......... .......... .......... 218 AAGTTATGAG GAGTTGTTTT CAACTTATTA GCTCTTGCTT TATTGGTTTA TATAGATTTG 22033 .......... .......... .......... .......... .......... .......... 218 GAATGACAAG ATAAGCATAC AGTGCGAAAT TTCAGTCTTC TTTGTTCACT TCTATCTTTA 22093 .......... .......... .......... .......... .......... .......... 218 GGGTGTGAAG AGAGATGCCT CTGCTGTGTA GTTAGCTGGG TTTAACTTGT TACATGAATG 22153 .......... .......... .......... .......... .......... .......... 218 TGGTTGATCT ACACAAGCAT CTTGTATTGC ATACACTTTC CAACCATCTA AGTGCTATTA 22213 .......... .......... .......... .......... .......... .......... 218 GTATAGATGA TTTTGACTTA TCAGAAAGGA AAATAAAAGA TGAGCAGAGG TGTGAGATTG 22273 .......... .......... .......... .......... .......... .......... 218 TGATATATGG TATAAACTTG GTGCTAGCAA GAAACTAAGG CTTGTTGTTG CATTATTATC 22333 .......... .......... .......... .......... .......... .......... 218 TAAACTTGGG GCTTGTTTTT GTTTCTTTAT TGTCAAAAAG TTTTGTTTTA GGTCTGTTTT 22393 .......... .......... .......... .......... .......... .......... 218 GATATTTTCA ATATAGTATG TTGAGAGTAG TTCTTTTTTG GGACTATACA GTTTGAATAA 22453 .......... .......... .......... .......... .......... .......... 218 CCTGAATAAA ATCTTGGATA ATTGAAGTTC TTTTTATTCT TTATAGGATG CTAAGGGCAA 22513 |||| |||| ||||| .......... .......... .......... .......... ......GATG CTAATGGCAA 232 GGACGTTGAT CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA 22573 |||||||||| |||||||||| |||| ||||| ||| |||||| |||||||||| |||||||||| GGACGTTGAT CTCAGTATTT ACAAAGGAAA AGTACTTATC ATTGTCAATG TTGCATCACA 292 GTGGTATGTA TTAGTTTCGT AGTTATTTTA TTCATTATAT GTTTGAAGCC TCTTCACAAT 22633 ||| GTG....... .......... .......... .......... .......... .......... 295 ATTTTGGATT TCAATAGGAA CTTTCGATGA GAGAAATGAT CTGATCGCTA GTTTTTGCTC 22693 .......... .......... .......... .......... .......... .......... 295 CTTGTAATTA AGTGAAATTT TCATGTGTTG CTCTCGATAG AATTGACTCA AAGTAAAGAA 22753 .......... .......... .......... .......... .......... .......... 295 GTATTAAACT TGAACCTTAT GCTACATCAG ATAGGGCATT CCTCCCCGTG ATGAGAATAC 22813 .......... .......... .......... .......... .......... .......... 295 TATGCATTGT GATAATAACT GAATTTACGA GATATTTAGT GAAGTTTTCA GGTGTTGTCC 22873 .......... .......... .......... .......... .......... .......... 295 TCCATAGAAT TGACTGGAAG TAAAAAAGTA TTAAAATTGA ACCTTATCTT ACACTGGATA 22933 .......... .......... .......... .......... .......... .......... 295 GGGCGTTCCT CCTTGTGATG AAAATACTAT GCATTTTTAT AAAAACTGAA TCTACGAGAA 22993 .......... .......... .......... .......... .......... .......... 295 ATTGAACATG ACATTATTTT ACTCGAGCCC TGACTAAACA TTCTTACTAT GCTTGGTCCA 23053 .......... .......... .......... .......... .......... .......... 295 CTACTCAACA AACGTGTTCT TAGTACCACT AATGACAAGT GATTGTGCAT ATCCAGATAA 23113 .......... .......... .......... .......... .......... .......... 295 GATGTCTTTT TCATTTCGGT ATAATTTGAT TTCATTGTGT TTACTGTGAA AGGTTTGAGC 23173 .......... .......... .......... .......... .......... .......... 295 AGTTACTTGT TGTATTTATG ATATCATTCT TTGATCTTGT TACATTTGCT TATGACTGTT 23233 .......... .......... .......... .......... .......... .......... 295 AACTGATTCC ATTTTCTGAT GCAGTGGCCT GACTAATTCG AACTACACTG ACATGA 23289 ||| || |||||||||| |||||||||| |||||| .......... .......... ....TGGGCT GACTAATTCG AACTACACTG ACATGA 327 hqPGS_C08HBa0251H04.1+_SGN-E335811+ (21314 21531,22500 22576,23258 23289) ******************************************************************************** EST sequence 32 +strand 609 n (File: SGN-E275539+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC 421 CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTTGACGTG 481 AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT 541 GGAGATGGTA TCAAGTGGAA CTTCTCCAAA TTCCTCATTG ACAAAGAAGG ACATGTCGTT 601 GATCGCTAC Predicted gene structure (within gDNA segment 20719 to 24449): Exon 1 21319 21531 ( 213 n); cDNA 1 213 ( 213 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 214 290 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 291 352 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 353 471 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23839 ( 138 n); cDNA 472 609 ( 138 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E275539+ 1.000 609 1.000 C PGS_C08HBa0251H04.1+_SGN-E275539+ (21319 21531,22500 22576,23258 23319,23469 23587,23702 23839) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 21378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 21438 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 21498 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 180 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA TTTTGATTTG 21558 |||||||||| |||||||||| |||||||||| ||| AACCCTCAAT CTGTTTATGA TTTCACTGTC AAG....... .......... .......... 213 GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA AAGAATGTTG 21618 .......... .......... .......... .......... .......... .......... 213 TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT GTGGTTTTTA 21678 .......... .......... .......... .......... .......... .......... 213 TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG AATCAGTAAC 21738 .......... .......... .......... .......... .......... .......... 213 ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA AGGTTGCTCA 21798 .......... .......... .......... .......... .......... .......... 213 TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA GTGTGAGCCT 21858 .......... .......... .......... .......... .......... .......... 213 TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC CCTGAGCTGG 21918 .......... .......... .......... .......... .......... .......... 213 AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG GCTTTAAGTT 21978 .......... .......... .......... .......... .......... .......... 213 ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG ATTTGGAATG 22038 .......... .......... .......... .......... .......... .......... 213 ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT CTTTAGGGTG 22098 .......... .......... .......... .......... .......... .......... 213 TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT GAATGTGGTT 22158 .......... .......... .......... .......... .......... .......... 213 GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC TATTAGTATA 22218 .......... .......... .......... .......... .......... .......... 213 GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA GATTGTGATA 22278 .......... .......... .......... .......... .......... .......... 213 TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA TTATCTAAAC 22338 .......... .......... .......... .......... .......... .......... 213 TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT GTTTTGATAT 22398 .......... .......... .......... .......... .......... .......... 213 TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG AATAACCTGA 22458 .......... .......... .......... .......... .......... .......... 213 ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG GGCAAGGACG 22518 ||||||||| |||||||||| .......... .......... .......... .......... .GATGCTAAG GGCAAGGACG 232 TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGGT 22578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTG.. 290 ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC ACAATATTTT 22638 .......... .......... .......... .......... .......... .......... 290 GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT TGCTCCTTGT 22698 .......... .......... .......... .......... .......... .......... 290 AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA AAGAAGTATT 22758 .......... .......... .......... .......... .......... .......... 290 AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG AATACTATGC 22818 .......... .......... .......... .......... .......... .......... 290 ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT TGTCCTCCAT 22878 .......... .......... .......... .......... .......... .......... 290 AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT GGATAGGGCG 22938 .......... .......... .......... .......... .......... .......... 290 TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC GAGAAATTGA 22998 .......... .......... .......... .......... .......... .......... 290 ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG GTCCACTACT 23058 .......... .......... .......... .......... .......... .......... 290 CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA GATAAGATGT 23118 .......... .......... .......... .......... .......... .......... 290 CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT TGAGCAGTTA 23178 .......... .......... .......... .......... .......... .......... 290 CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA CTGTTAACTG 23238 .......... .......... .......... .......... .......... .......... 290 ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 23298 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 331 ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG CCCTCGATTC 23358 |||||||||| |||||||||| | ACAAGAAGTA CAAGGATCAA G......... .......... .......... .......... 352 TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA ATAAAATGGA 23418 .......... .......... .......... .......... .......... .......... 352 TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG GTTTGGAGAT 23478 |||||||||| .......... .......... .......... .......... .......... GTTTGGAGAT 362 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 23538 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 422 GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG TAGAAATAAC 23598 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAG. .......... 471 ACGAAGCATT CTTCTCTTCT AGTCATCTAC ACTACAGCTG AAACTGAGAA TAAATGAGGC 23658 .......... .......... .......... .......... .......... .......... 471 AAGCTGTATG TACTGACATC TTCGTGTGTT TTGTGTGGTC TAGGTTGACG TGAATGGTGA 23718 ||||||| |||||||||| .......... .......... .......... .......... ...GTTGACG TGAATGGTGA 488 TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 23778 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 548 TATCAAGTGG AACTTCTCCA AATTCCTCAT TGACAAAGAA GGACATGTCG TTGATCGCTA 23838 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATCAAGTGG AACTTCTCCA AATTCCTCAT TGACAAAGAA GGACATGTCG TTGATCGCTA 608 C 23839 | C 609 hqPGS_C08HBa0251H04.1+_SGN-E275539+ (21319 21531,22500 22576,23258 23319,23469 23587,23702 23839) ******************************************************************************** EST sequence 40 +strand 582 n (File: SGN-E308395+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTGCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC 421 CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTTGACGTG 481 AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT 541 GGAGATGGTA TCAAGTGGAA CTTCTCCAAA TTCCTCATTG AC Predicted gene structure (within gDNA segment 20719 to 24422): Exon 1 21319 21531 ( 213 n); cDNA 1 213 ( 213 n); score: 0.995 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 214 290 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 291 352 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 353 471 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23812 ( 111 n); cDNA 472 582 ( 111 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E308395+ 0.998 582 1.000 C PGS_C08HBa0251H04.1+_SGN-E308395+ (21319 21531,22500 22576,23258 23319,23469 23587,23702 23812) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 21378 |||||||||| |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTGCAATTC TGTGTTTAAT 60 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 21438 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 21498 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 180 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA TTTTGATTTG 21558 |||||||||| |||||||||| |||||||||| ||| AACCCTCAAT CTGTTTATGA TTTCACTGTC AAG....... .......... .......... 213 GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA AAGAATGTTG 21618 .......... .......... .......... .......... .......... .......... 213 TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT GTGGTTTTTA 21678 .......... .......... .......... .......... .......... .......... 213 TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG AATCAGTAAC 21738 .......... .......... .......... .......... .......... .......... 213 ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA AGGTTGCTCA 21798 .......... .......... .......... .......... .......... .......... 213 TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA GTGTGAGCCT 21858 .......... .......... .......... .......... .......... .......... 213 TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC CCTGAGCTGG 21918 .......... .......... .......... .......... .......... .......... 213 AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG GCTTTAAGTT 21978 .......... .......... .......... .......... .......... .......... 213 ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG ATTTGGAATG 22038 .......... .......... .......... .......... .......... .......... 213 ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT CTTTAGGGTG 22098 .......... .......... .......... .......... .......... .......... 213 TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT GAATGTGGTT 22158 .......... .......... .......... .......... .......... .......... 213 GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC TATTAGTATA 22218 .......... .......... .......... .......... .......... .......... 213 GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA GATTGTGATA 22278 .......... .......... .......... .......... .......... .......... 213 TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA TTATCTAAAC 22338 .......... .......... .......... .......... .......... .......... 213 TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT GTTTTGATAT 22398 .......... .......... .......... .......... .......... .......... 213 TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG AATAACCTGA 22458 .......... .......... .......... .......... .......... .......... 213 ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG GGCAAGGACG 22518 ||||||||| |||||||||| .......... .......... .......... .......... .GATGCTAAG GGCAAGGACG 232 TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGGT 22578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTG.. 290 ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC ACAATATTTT 22638 .......... .......... .......... .......... .......... .......... 290 GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT TGCTCCTTGT 22698 .......... .......... .......... .......... .......... .......... 290 AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA AAGAAGTATT 22758 .......... .......... .......... .......... .......... .......... 290 AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG AATACTATGC 22818 .......... .......... .......... .......... .......... .......... 290 ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT TGTCCTCCAT 22878 .......... .......... .......... .......... .......... .......... 290 AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT GGATAGGGCG 22938 .......... .......... .......... .......... .......... .......... 290 TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC GAGAAATTGA 22998 .......... .......... .......... .......... .......... .......... 290 ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG GTCCACTACT 23058 .......... .......... .......... .......... .......... .......... 290 CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA GATAAGATGT 23118 .......... .......... .......... .......... .......... .......... 290 CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT TGAGCAGTTA 23178 .......... .......... .......... .......... .......... .......... 290 CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA CTGTTAACTG 23238 .......... .......... .......... .......... .......... .......... 290 ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 23298 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 331 ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG CCCTCGATTC 23358 |||||||||| |||||||||| | ACAAGAAGTA CAAGGATCAA G......... .......... .......... .......... 352 TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA ATAAAATGGA 23418 .......... .......... .......... .......... .......... .......... 352 TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG GTTTGGAGAT 23478 |||||||||| .......... .......... .......... .......... .......... GTTTGGAGAT 362 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 23538 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 422 GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG TAGAAATAAC 23598 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAG. .......... 471 ACGAAGCATT CTTCTCTTCT AGTCATCTAC ACTACAGCTG AAACTGAGAA TAAATGAGGC 23658 .......... .......... .......... .......... .......... .......... 471 AAGCTGTATG TACTGACATC TTCGTGTGTT TTGTGTGGTC TAGGTTGACG TGAATGGTGA 23718 ||||||| |||||||||| .......... .......... .......... .......... ...GTTGACG TGAATGGTGA 488 TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 23778 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 548 TATCAAGTGG AACTTCTCCA AATTCCTCAT TGAC 23812 |||||||||| |||||||||| |||||||||| |||| TATCAAGTGG AACTTCTCCA AATTCCTCAT TGAC 582 hqPGS_C08HBa0251H04.1+_SGN-E308395+ (21319 21531,22500 22576,23258 23319,23469 23587,23702 23812) ******************************************************************************** EST sequence 24 +strand 411 n (File: SGN-E291487+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT T Predicted gene structure (within gDNA segment 20719 to 24137): Exon 1 21319 21531 ( 213 n); cDNA 1 213 ( 213 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 214 290 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 291 352 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23527 ( 59 n); cDNA 353 411 ( 59 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E291487+ 1.000 411 1.000 C PGS_C08HBa0251H04.1+_SGN-E291487+ (21319 21531,22500 22576,23258 23319,23469 23527) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 21378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 21438 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 21498 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 180 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA TTTTGATTTG 21558 |||||||||| |||||||||| |||||||||| ||| AACCCTCAAT CTGTTTATGA TTTCACTGTC AAG....... .......... .......... 213 GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA AAGAATGTTG 21618 .......... .......... .......... .......... .......... .......... 213 TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT GTGGTTTTTA 21678 .......... .......... .......... .......... .......... .......... 213 TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG AATCAGTAAC 21738 .......... .......... .......... .......... .......... .......... 213 ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA AGGTTGCTCA 21798 .......... .......... .......... .......... .......... .......... 213 TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA GTGTGAGCCT 21858 .......... .......... .......... .......... .......... .......... 213 TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC CCTGAGCTGG 21918 .......... .......... .......... .......... .......... .......... 213 AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG GCTTTAAGTT 21978 .......... .......... .......... .......... .......... .......... 213 ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG ATTTGGAATG 22038 .......... .......... .......... .......... .......... .......... 213 ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT CTTTAGGGTG 22098 .......... .......... .......... .......... .......... .......... 213 TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT GAATGTGGTT 22158 .......... .......... .......... .......... .......... .......... 213 GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC TATTAGTATA 22218 .......... .......... .......... .......... .......... .......... 213 GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA GATTGTGATA 22278 .......... .......... .......... .......... .......... .......... 213 TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA TTATCTAAAC 22338 .......... .......... .......... .......... .......... .......... 213 TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT GTTTTGATAT 22398 .......... .......... .......... .......... .......... .......... 213 TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG AATAACCTGA 22458 .......... .......... .......... .......... .......... .......... 213 ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG GGCAAGGACG 22518 ||||||||| |||||||||| .......... .......... .......... .......... .GATGCTAAG GGCAAGGACG 232 TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGGT 22578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTG.. 290 ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC ACAATATTTT 22638 .......... .......... .......... .......... .......... .......... 290 GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT TGCTCCTTGT 22698 .......... .......... .......... .......... .......... .......... 290 AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA AAGAAGTATT 22758 .......... .......... .......... .......... .......... .......... 290 AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG AATACTATGC 22818 .......... .......... .......... .......... .......... .......... 290 ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT TGTCCTCCAT 22878 .......... .......... .......... .......... .......... .......... 290 AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT GGATAGGGCG 22938 .......... .......... .......... .......... .......... .......... 290 TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC GAGAAATTGA 22998 .......... .......... .......... .......... .......... .......... 290 ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG GTCCACTACT 23058 .......... .......... .......... .......... .......... .......... 290 CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA GATAAGATGT 23118 .......... .......... .......... .......... .......... .......... 290 CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT TGAGCAGTTA 23178 .......... .......... .......... .......... .......... .......... 290 CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA CTGTTAACTG 23238 .......... .......... .......... .......... .......... .......... 290 ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 23298 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 331 ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG CCCTCGATTC 23358 |||||||||| |||||||||| | ACAAGAAGTA CAAGGATCAA G......... .......... .......... .......... 352 TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA ATAAAATGGA 23418 .......... .......... .......... .......... .......... .......... 352 TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG GTTTGGAGAT 23478 |||||||||| .......... .......... .......... .......... .......... GTTTGGAGAT 362 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATT 23527 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATT 411 hqPGS_C08HBa0251H04.1+_SGN-E291487+ (21319 21531,22500 22576,23258 23319,23469 23527) ******************************************************************************** EST sequence 28 +strand 395 n (File: SGN-E232753+) 1 AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 61 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 121 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 181 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 241 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 301 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 361 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACA Predicted gene structure (within gDNA segment 20719 to 24121): Exon 1 21319 21531 ( 213 n); cDNA 1 213 ( 213 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 214 290 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 291 352 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23511 ( 43 n); cDNA 353 395 ( 43 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E232753+ 1.000 395 1.000 C PGS_C08HBa0251H04.1+_SGN-E232753+ (21319 21531,22500 22576,23258 23319,23469 23511) Alignment (genomic DNA sequence = upper lines): AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 21378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATTTTAA GGCGATTTTC TTCGATTCTA AGACAAACCC AGTTCAATTC TGTGTTTAAT 60 CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 21438 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCTGTTT CGTCTTTCTC TAAGCCGATC CGAACAGTTT TACCCAATTC TTTGGTTACC 120 TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 21498 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTAAGGGTT TTGAGTTGTG GGGTTTAAGA TCAGATCATA CCATGGCTAG TCAATCAAGC 180 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA TTTTGATTTG 21558 |||||||||| |||||||||| |||||||||| ||| AACCCTCAAT CTGTTTATGA TTTCACTGTC AAG....... .......... .......... 213 GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA AAGAATGTTG 21618 .......... .......... .......... .......... .......... .......... 213 TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT GTGGTTTTTA 21678 .......... .......... .......... .......... .......... .......... 213 TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG AATCAGTAAC 21738 .......... .......... .......... .......... .......... .......... 213 ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA AGGTTGCTCA 21798 .......... .......... .......... .......... .......... .......... 213 TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA GTGTGAGCCT 21858 .......... .......... .......... .......... .......... .......... 213 TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC CCTGAGCTGG 21918 .......... .......... .......... .......... .......... .......... 213 AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG GCTTTAAGTT 21978 .......... .......... .......... .......... .......... .......... 213 ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG ATTTGGAATG 22038 .......... .......... .......... .......... .......... .......... 213 ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT CTTTAGGGTG 22098 .......... .......... .......... .......... .......... .......... 213 TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT GAATGTGGTT 22158 .......... .......... .......... .......... .......... .......... 213 GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC TATTAGTATA 22218 .......... .......... .......... .......... .......... .......... 213 GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA GATTGTGATA 22278 .......... .......... .......... .......... .......... .......... 213 TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA TTATCTAAAC 22338 .......... .......... .......... .......... .......... .......... 213 TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT GTTTTGATAT 22398 .......... .......... .......... .......... .......... .......... 213 TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG AATAACCTGA 22458 .......... .......... .......... .......... .......... .......... 213 ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG GGCAAGGACG 22518 ||||||||| |||||||||| .......... .......... .......... .......... .GATGCTAAG GGCAAGGACG 232 TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGGT 22578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTG.. 290 ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC ACAATATTTT 22638 .......... .......... .......... .......... .......... .......... 290 GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT TGCTCCTTGT 22698 .......... .......... .......... .......... .......... .......... 290 AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA AAGAAGTATT 22758 .......... .......... .......... .......... .......... .......... 290 AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG AATACTATGC 22818 .......... .......... .......... .......... .......... .......... 290 ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT TGTCCTCCAT 22878 .......... .......... .......... .......... .......... .......... 290 AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT GGATAGGGCG 22938 .......... .......... .......... .......... .......... .......... 290 TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC GAGAAATTGA 22998 .......... .......... .......... .......... .......... .......... 290 ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG GTCCACTACT 23058 .......... .......... .......... .......... .......... .......... 290 CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA GATAAGATGT 23118 .......... .......... .......... .......... .......... .......... 290 CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT TGAGCAGTTA 23178 .......... .......... .......... .......... .......... .......... 290 CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA CTGTTAACTG 23238 .......... .......... .......... .......... .......... .......... 290 ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 23298 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 331 ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG CCCTCGATTC 23358 |||||||||| |||||||||| | ACAAGAAGTA CAAGGATCAA G......... .......... .......... .......... 352 TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA ATAAAATGGA 23418 .......... .......... .......... .......... .......... .......... 352 TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG GTTTGGAGAT 23478 |||||||||| .......... .......... .......... .......... .......... GTTTGGAGAT 362 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACA 23511 |||||||||| |||||||||| |||||||||| ||| TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACA 395 hqPGS_C08HBa0251H04.1+_SGN-E232753+ (21319 21531,22500 22576,23258 23319,23469 23511) ******************************************************************************** EST sequence 6 +strand 508 n (File: SGN-E295010+) 1 TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 61 TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 121 TAAGGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC AATCAAGCAA 181 CCCTCAATCT GTTTATGATT TCACTGTCAA GGATGCTAAG GGCAAGGACG TTGATCTCAG 241 TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGTG GCCTGACTAA 301 TTCGAACTAC ACTGACATGA CGGAGTTATA CAAGAAGTAC AAGGATCAAG GTTTGGAGAT 361 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 421 GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG TTGACGTGAA 481 TGGTGATAAT GCTGCTCCAT TATATAGG Predicted gene structure (within gDNA segment 20721 to 24350): Exon 1 21321 21531 ( 211 n); cDNA 1 211 ( 211 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 212 288 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 289 350 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 351 469 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 0) Exon 5 23702 23740 ( 39 n); cDNA 470 508 ( 39 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E295010+ 1.000 508 1.000 C PGS_C08HBa0251H04.1+_SGN-E295010+ (21321 21531,22500 22576,23258 23319,23469 23587,23702 23740) Alignment (genomic DNA sequence = upper lines): TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 21380 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATTTTAAGG CGATTTTCTT CGATTCTAAG ACAAACCCAG TTCAATTCTG TGTTTAATCT 60 TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 21440 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCTGTTTCG TCTTTCTCTA AGCCGATCCG AACAGTTTTA CCCAATTCTT TGGTTACCTC 120 TAAGGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC AATCAAGCAA 21500 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAGGGTTTT GAGTTGTGGG GTTTAAGATC AGATCATACC ATGGCTAGTC AATCAAGCAA 180 CCCTCAATCT GTTTATGATT TCACTGTCAA GGTTAGGACT TTTGCTTATT TTGATTTGGG 21560 |||||||||| |||||||||| |||||||||| | CCCTCAATCT GTTTATGATT TCACTGTCAA G......... .......... .......... 211 GTTTTCCTGA AATGTTCAAT TTTGCTTATT TTGACTGATT GGTTTAGAAA GAATGTTGTT 21620 .......... .......... .......... .......... .......... .......... 211 TTTGTTTGTT TTGTTAGTGT TTGGGGTTAG GAAGTTGAGT TATGTGGTGT GGTTTTTATC 21680 .......... .......... .......... .......... .......... .......... 211 ATTTTTTGGG TTCGTGGGCG GTTAAAATTT GGTTTTATAT TGGGTATGAA TCAGTAACAT 21740 .......... .......... .......... .......... .......... .......... 211 AAGCAATGAA TGTGAAAGAT TATTTATTTT TGTTTTCTTT TGTGTTAAAG GTTGCTCATT 21800 .......... .......... .......... .......... .......... .......... 211 TATTAATGCT GTTGTTTAGT TTTTTTGGTT TATGGTCGTA CCTTATGAGT GTGAGCCTTA 21860 .......... .......... .......... .......... .......... .......... 211 GAAACACGGT AAGGCTGCTT GCAATAGACT CTTGTGGCTT GGCCCTTCCC TGAGCTGGAG 21920 .......... .......... .......... .......... .......... .......... 211 CTTAGTGCAT TGGCTTGCTT TTTTGTCTTT TGCAATTAGA GATTTGGGGC TTTAAGTTAT 21980 .......... .......... .......... .......... .......... .......... 211 GAGGAGTTGT TTTCAACTTA TTAGCTCTTG CTTTATTGGT TTATATAGAT TTGGAATGAC 22040 .......... .......... .......... .......... .......... .......... 211 AAGATAAGCA TACAGTGCGA AATTTCAGTC TTCTTTGTTC ACTTCTATCT TTAGGGTGTG 22100 .......... .......... .......... .......... .......... .......... 211 AAGAGAGATG CCTCTGCTGT GTAGTTAGCT GGGTTTAACT TGTTACATGA ATGTGGTTGA 22160 .......... .......... .......... .......... .......... .......... 211 TCTACACAAG CATCTTGTAT TGCATACACT TTCCAACCAT CTAAGTGCTA TTAGTATAGA 22220 .......... .......... .......... .......... .......... .......... 211 TGATTTTGAC TTATCAGAAA GGAAAATAAA AGATGAGCAG AGGTGTGAGA TTGTGATATA 22280 .......... .......... .......... .......... .......... .......... 211 TGGTATAAAC TTGGTGCTAG CAAGAAACTA AGGCTTGTTG TTGCATTATT ATCTAAACTT 22340 .......... .......... .......... .......... .......... .......... 211 GGGGCTTGTT TTTGTTTCTT TATTGTCAAA AAGTTTTGTT TTAGGTCTGT TTTGATATTT 22400 .......... .......... .......... .......... .......... .......... 211 TCAATATAGT ATGTTGAGAG TAGTTCTTTT TTGGGACTAT ACAGTTTGAA TAACCTGAAT 22460 .......... .......... .......... .......... .......... .......... 211 AAAATCTTGG ATAATTGAAG TTCTTTTTAT TCTTTATAGG ATGCTAAGGG CAAGGACGTT 22520 | |||||||||| |||||||||| .......... .......... .......... .........G ATGCTAAGGG CAAGGACGTT 232 GATCTCAGTA TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTGGTAT 22580 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| GATCTCAGTA TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTG.... 288 GTATTAGTTT CGTAGTTATT TTATTCATTA TATGTTTGAA GCCTCTTCAC AATATTTTGG 22640 .......... .......... .......... .......... .......... .......... 288 ATTTCAATAG GAACTTTCGA TGAGAGAAAT GATCTGATCG CTAGTTTTTG CTCCTTGTAA 22700 .......... .......... .......... .......... .......... .......... 288 TTAAGTGAAA TTTTCATGTG TTGCTCTCGA TAGAATTGAC TCAAAGTAAA GAAGTATTAA 22760 .......... .......... .......... .......... .......... .......... 288 ACTTGAACCT TATGCTACAT CAGATAGGGC ATTCCTCCCC GTGATGAGAA TACTATGCAT 22820 .......... .......... .......... .......... .......... .......... 288 TGTGATAATA ACTGAATTTA CGAGATATTT AGTGAAGTTT TCAGGTGTTG TCCTCCATAG 22880 .......... .......... .......... .......... .......... .......... 288 AATTGACTGG AAGTAAAAAA GTATTAAAAT TGAACCTTAT CTTACACTGG ATAGGGCGTT 22940 .......... .......... .......... .......... .......... .......... 288 CCTCCTTGTG ATGAAAATAC TATGCATTTT TATAAAAACT GAATCTACGA GAAATTGAAC 23000 .......... .......... .......... .......... .......... .......... 288 ATGACATTAT TTTACTCGAG CCCTGACTAA ACATTCTTAC TATGCTTGGT CCACTACTCA 23060 .......... .......... .......... .......... .......... .......... 288 ACAAACGTGT TCTTAGTACC ACTAATGACA AGTGATTGTG CATATCCAGA TAAGATGTCT 23120 .......... .......... .......... .......... .......... .......... 288 TTTTCATTTC GGTATAATTT GATTTCATTG TGTTTACTGT GAAAGGTTTG AGCAGTTACT 23180 .......... .......... .......... .......... .......... .......... 288 TGTTGTATTT ATGATATCAT TCTTTGATCT TGTTACATTT GCTTATGACT GTTAACTGAT 23240 .......... .......... .......... .......... .......... .......... 288 TCCATTTTCT GATGCAGTGG CCTGACTAAT TCGAACTACA CTGACATGAC GGAGTTATAC 23300 ||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .......TGG CCTGACTAAT TCGAACTACA CTGACATGAC GGAGTTATAC 331 AAGAAGTACA AGGATCAAGG TTTGTCCAAT TCTCCCGAAC CAACTGTGCC CTCGATTCTC 23360 |||||||||| ||||||||| AAGAAGTACA AGGATCAAG. .......... .......... .......... .......... 350 CAGCAATGGA GGCGTTCGCC CTGACATATT TCTGCAAAGA GAACTTAAAT AAAATGGATG 23420 .......... .......... .......... .......... .......... .......... 350 TTTATATCTA ATTTAGCTTG TTAATTATAT GTTCTTCCTG TTACACAGGT TTGGAGATTC 23480 || |||||||||| .......... .......... .......... .......... ........GT TTGGAGATTC 362 TGGCCTTCCC TTGCAACCAG TTTGGTGGAC AGGAGCCTGG AAACATTGAA GATATCCAGC 23540 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGCCTTCCC TTGCAACCAG TTTGGTGGAC AGGAGCCTGG AAACATTGAA GATATCCAGC 422 AAATGGTCTG CACTCGCTTC AAGGCTGAGT ACCCCATATT TGACAAGGTA GAAATAACAC 23600 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| AAATGGTCTG CACTCGCTTC AAGGCTGAGT ACCCCATATT TGACAAG... .......... 469 GAAGCATTCT TCTCTTCTAG TCATCTACAC TACAGCTGAA ACTGAGAATA AATGAGGCAA 23660 .......... .......... .......... .......... .......... .......... 469 GCTGTATGTA CTGACATCTT CGTGTGTTTT GTGTGGTCTA GGTTGACGTG AATGGTGATA 23720 ||||||||| |||||||||| .......... .......... .......... .......... .GTTGACGTG AATGGTGATA 488 ATGCTGCTCC ATTATATAGG 23740 |||||||||| |||||||||| ATGCTGCTCC ATTATATAGG 508 hqPGS_C08HBa0251H04.1+_SGN-E295010+ (21321 21531,22500 22576,23258 23319,23469 23587,23702 23740) ******************************************************************************** EST sequence 9 +strand 638 n (File: SGN-E371609+) 1 TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 61 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 121 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 181 CTCAATCTGT TTATGATTTC ACTGTCAAGG ATGCTAAGGG CAAGGACGTT GATCTCAGTA 241 TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTGGTAT GTATTAGTTT 301 CGTAGTTATT TTATTCATTA TATGTTTGAA GCCTCTTCAC AATATTTTGG ATTTCAATAG 361 GAACTTTCGA TGAGAGAAAT GATCTGATCG CTAGTTTTTG CTCCTTGTAA TTAAGTGAAA 421 TTTTCATGTG TTGCTCTCGA TAGAATTGAC TCAAAGTAAA GAAGTATTAA ACTTGAACCT 481 TATGCTACAT CAGATAGGGC ATTCCTCCCC GTGATGAGAA TACTATGCAT TGTGATAATA 541 ACTGAATTTA CGAGATATTT AGTGAAGTTT TCAGGTGTTG TCCTCCATAG AATTGACTGG 601 AAGTAAAAAA GTATTAAAAT TGAACCTTAT CTTACACT Predicted gene structure (within gDNA segment 20624 to 23538): Exon 1 21324 21531 ( 208 n); cDNA 1 209 ( 209 n); score: 0.988 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22928 ( 429 n); cDNA 210 638 ( 429 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E371609+ 0.996 637 0.998 C PGS_C08HBa0251H04.1+_SGN-E371609+ (21324 21531,22500 22928) Alignment (genomic DNA sequence = upper lines): TTTAAGGCGA -TTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 21382 |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 60 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 21442 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 120 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 21502 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 180 CTCAATCTGT TTATGATTTC ACTGTCAAGG TTAGGACTTT TGCTTATTTT GATTTGGGGT 21562 |||||||||| |||||||||| ||||||||| CTCAATCTGT TTATGATTTC ACTGTCAAG. .......... .......... .......... 209 TTTCCTGAAA TGTTCAATTT TGCTTATTTT GACTGATTGG TTTAGAAAGA ATGTTGTTTT 21622 .......... .......... .......... .......... .......... .......... 209 TGTTTGTTTT GTTAGTGTTT GGGGTTAGGA AGTTGAGTTA TGTGGTGTGG TTTTTATCAT 21682 .......... .......... .......... .......... .......... .......... 209 TTTTTGGGTT CGTGGGCGGT TAAAATTTGG TTTTATATTG GGTATGAATC AGTAACATAA 21742 .......... .......... .......... .......... .......... .......... 209 GCAATGAATG TGAAAGATTA TTTATTTTTG TTTTCTTTTG TGTTAAAGGT TGCTCATTTA 21802 .......... .......... .......... .......... .......... .......... 209 TTAATGCTGT TGTTTAGTTT TTTTGGTTTA TGGTCGTACC TTATGAGTGT GAGCCTTAGA 21862 .......... .......... .......... .......... .......... .......... 209 AACACGGTAA GGCTGCTTGC AATAGACTCT TGTGGCTTGG CCCTTCCCTG AGCTGGAGCT 21922 .......... .......... .......... .......... .......... .......... 209 TAGTGCATTG GCTTGCTTTT TTGTCTTTTG CAATTAGAGA TTTGGGGCTT TAAGTTATGA 21982 .......... .......... .......... .......... .......... .......... 209 GGAGTTGTTT TCAACTTATT AGCTCTTGCT TTATTGGTTT ATATAGATTT GGAATGACAA 22042 .......... .......... .......... .......... .......... .......... 209 GATAAGCATA CAGTGCGAAA TTTCAGTCTT CTTTGTTCAC TTCTATCTTT AGGGTGTGAA 22102 .......... .......... .......... .......... .......... .......... 209 GAGAGATGCC TCTGCTGTGT AGTTAGCTGG GTTTAACTTG TTACATGAAT GTGGTTGATC 22162 .......... .......... .......... .......... .......... .......... 209 TACACAAGCA TCTTGTATTG CATACACTTT CCAACCATCT AAGTGCTATT AGTATAGATG 22222 .......... .......... .......... .......... .......... .......... 209 ATTTTGACTT ATCAGAAAGG AAAATAAAAG ATGAGCAGAG GTGTGAGATT GTGATATATG 22282 .......... .......... .......... .......... .......... .......... 209 GTATAAACTT GGTGCTAGCA AGAAACTAAG GCTTGTTGTT GCATTATTAT CTAAACTTGG 22342 .......... .......... .......... .......... .......... .......... 209 GGCTTGTTTT TGTTTCTTTA TTGTCAAAAA GTTTTGTTTT AGGTCTGTTT TGATATTTTC 22402 .......... .......... .......... .......... .......... .......... 209 AATATAGTAT GTTGAGAGTA GTTCTTTTTT GGGACTATAC AGTTTGAATA ACCTGAATAA 22462 .......... .......... .......... .......... .......... .......... 209 AATCTTGGAT AATTGAAGTT CTTTTTATTC TTTATAGGAT GCTAAGGGCA AGGACGTTGA 22522 ||| |||||||||| |||||||||| .......... .......... .......... .......GAT GCTAAGGGCA AGGACGTTGA 232 TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGGTATGT 22582 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGGTATGT 292 ATTAGTTTCG TAGTTATTTT ATTCATTATA TGTTTGAAGC CTCTTCACAA TATTTTGGAT 22642 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTAGTTTCG TAGTTATTTT ATTCATTATA TGTTTGAAGC CTCTTCACAA TATTTTGGAT 352 TTCAATAGGA ACTTTCGATG AGAGAAATGA TCTGATCGCT AGTTTTTGCT CCTTGTAATT 22702 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCAATAGGA ACTTTCGATG AGAGAAATGA TCTGATCGCT AGTTTTTGCT CCTTGTAATT 412 AAGTGAAATT TTCATGTGTT GCTCTCGATA GAATTGACTC AAAGTAAAGA AGTATTAAAC 22762 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGAAATT TTCATGTGTT GCTCTCGATA GAATTGACTC AAAGTAAAGA AGTATTAAAC 472 TTGAACCTTA TGCTACATCA GATAGGGCAT TCCTCCCCGT GATGAGAATA CTATGCATTG 22822 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGAACCTTA TGCTACATCA GATAGGGCAT TCCTCCCCGT GATGAGAATA CTATGCATTG 532 TGATAATAAC TGAATTTACG AGATATTTAG TGAAGTTTTC AGGTGTTGTC CTCCATAGAA 22882 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATAATAAC TGAATTTACG AGATATTTAG TGAAGTTTTC AGGTGTTGTC CTCCATAGAA 592 TTGACTGGAA GTAAAAAAGT ATTAAAATTG AACCTTATCT TACACT 22928 |||||||||| |||||||||| |||||||||| |||||||||| |||||| TTGACTGGAA GTAAAAAAGT ATTAAAATTG AACCTTATCT TACACT 638 hqPGS_C08HBa0251H04.1+_SGN-E371609+ (21324 21531,22500 22928) ******************************************************************************** EST sequence 15 +strand 529 n (File: SGN-E280323+) 1 TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 61 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 121 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 181 CTCAATCTGT TTATGATTTC ACTGTCAAGG ATGCTAAGGG CAAGGACGTT GATCTCAGTA 241 TTTACAAGGG AAAAGTCCTT ATCATTGTCA ATGTTGCATC ACAGTGGTAT GTATTAGTTT 301 CGTAGTTATT TTATTCATTA TATGTTTGAA GCCTCTTCAC AATATTTTGG ATTTCAATAG 361 GAACTTTCGA TGAGAGAAAT GATCTGATCG CTAGTTTTTG CTCCTTGTAA TTAAGTGAAA 421 TTTTCATGTG TTGCTCTCGA TAGAATTGAC TCAAAGTAAA GAAGTATTAA ACTTGAACCT 481 TATGCTACAT CAGATAGGGC ATTCCTCCCC GTGATGAGAA TACTATGCA Predicted gene structure (within gDNA segment 20624 to 23429): Exon 1 21324 21531 ( 208 n); cDNA 1 209 ( 209 n); score: 0.988 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22819 ( 320 n); cDNA 210 529 ( 320 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E280323+ 0.995 528 0.998 C PGS_C08HBa0251H04.1+_SGN-E280323+ (21324 21531,22500 22819) Alignment (genomic DNA sequence = upper lines): TTTAAGGCGA -TTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 21382 |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTTCTTCG ATTCTAAGAC AAACCCAGTT CAATTCTGTG TTTAATCTTC 60 CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 21442 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTTCGTC TTTCTCTAAG CCGATCCGAA CAGTTTTACC CAATTCTTTG GTTACCTCTA 120 AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 21502 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGGTTTTGA GTTGTGGGGT TTAAGATCAG ATCATACCAT GGCTAGTCAA TCAAGCAACC 180 CTCAATCTGT TTATGATTTC ACTGTCAAGG TTAGGACTTT TGCTTATTTT GATTTGGGGT 21562 |||||||||| |||||||||| ||||||||| CTCAATCTGT TTATGATTTC ACTGTCAAG. .......... .......... .......... 209 TTTCCTGAAA TGTTCAATTT TGCTTATTTT GACTGATTGG TTTAGAAAGA ATGTTGTTTT 21622 .......... .......... .......... .......... .......... .......... 209 TGTTTGTTTT GTTAGTGTTT GGGGTTAGGA AGTTGAGTTA TGTGGTGTGG TTTTTATCAT 21682 .......... .......... .......... .......... .......... .......... 209 TTTTTGGGTT CGTGGGCGGT TAAAATTTGG TTTTATATTG GGTATGAATC AGTAACATAA 21742 .......... .......... .......... .......... .......... .......... 209 GCAATGAATG TGAAAGATTA TTTATTTTTG TTTTCTTTTG TGTTAAAGGT TGCTCATTTA 21802 .......... .......... .......... .......... .......... .......... 209 TTAATGCTGT TGTTTAGTTT TTTTGGTTTA TGGTCGTACC TTATGAGTGT GAGCCTTAGA 21862 .......... .......... .......... .......... .......... .......... 209 AACACGGTAA GGCTGCTTGC AATAGACTCT TGTGGCTTGG CCCTTCCCTG AGCTGGAGCT 21922 .......... .......... .......... .......... .......... .......... 209 TAGTGCATTG GCTTGCTTTT TTGTCTTTTG CAATTAGAGA TTTGGGGCTT TAAGTTATGA 21982 .......... .......... .......... .......... .......... .......... 209 GGAGTTGTTT TCAACTTATT AGCTCTTGCT TTATTGGTTT ATATAGATTT GGAATGACAA 22042 .......... .......... .......... .......... .......... .......... 209 GATAAGCATA CAGTGCGAAA TTTCAGTCTT CTTTGTTCAC TTCTATCTTT AGGGTGTGAA 22102 .......... .......... .......... .......... .......... .......... 209 GAGAGATGCC TCTGCTGTGT AGTTAGCTGG GTTTAACTTG TTACATGAAT GTGGTTGATC 22162 .......... .......... .......... .......... .......... .......... 209 TACACAAGCA TCTTGTATTG CATACACTTT CCAACCATCT AAGTGCTATT AGTATAGATG 22222 .......... .......... .......... .......... .......... .......... 209 ATTTTGACTT ATCAGAAAGG AAAATAAAAG ATGAGCAGAG GTGTGAGATT GTGATATATG 22282 .......... .......... .......... .......... .......... .......... 209 GTATAAACTT GGTGCTAGCA AGAAACTAAG GCTTGTTGTT GCATTATTAT CTAAACTTGG 22342 .......... .......... .......... .......... .......... .......... 209 GGCTTGTTTT TGTTTCTTTA TTGTCAAAAA GTTTTGTTTT AGGTCTGTTT TGATATTTTC 22402 .......... .......... .......... .......... .......... .......... 209 AATATAGTAT GTTGAGAGTA GTTCTTTTTT GGGACTATAC AGTTTGAATA ACCTGAATAA 22462 .......... .......... .......... .......... .......... .......... 209 AATCTTGGAT AATTGAAGTT CTTTTTATTC TTTATAGGAT GCTAAGGGCA AGGACGTTGA 22522 ||| |||||||||| |||||||||| .......... .......... .......... .......GAT GCTAAGGGCA AGGACGTTGA 232 TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGGTATGT 22582 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCAGTATT TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGGTATGT 292 ATTAGTTTCG TAGTTATTTT ATTCATTATA TGTTTGAAGC CTCTTCACAA TATTTTGGAT 22642 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTAGTTTCG TAGTTATTTT ATTCATTATA TGTTTGAAGC CTCTTCACAA TATTTTGGAT 352 TTCAATAGGA ACTTTCGATG AGAGAAATGA TCTGATCGCT AGTTTTTGCT CCTTGTAATT 22702 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCAATAGGA ACTTTCGATG AGAGAAATGA TCTGATCGCT AGTTTTTGCT CCTTGTAATT 412 AAGTGAAATT TTCATGTGTT GCTCTCGATA GAATTGACTC AAAGTAAAGA AGTATTAAAC 22762 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGTGAAATT TTCATGTGTT GCTCTCGATA GAATTGACTC AAAGTAAAGA AGTATTAAAC 472 TTGAACCTTA TGCTACATCA GATAGGGCAT TCCTCCCCGT GATGAGAATA CTATGCA 22819 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TTGAACCTTA TGCTACATCA GATAGGGCAT TCCTCCCCGT GATGAGAATA CTATGCA 529 hqPGS_C08HBa0251H04.1+_SGN-E280323+ (21324 21531,22500 22819) ******************************************************************************** EST sequence 45 +strand 284 n (File: SGN-E290421+) 1 TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 61 TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTCTAA 121 GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 181 TCAATCTGTT TATGATTTCA CTGTCAAGGA TGCTAAGGGC AAGGACGTTG ATCTCAGTAT 241 TTACAAGGGA AAAGTCCTTA TCATTGTCAA TGTTGCATCA CAGT Predicted gene structure (within gDNA segment 20724 to 23185): Exon 1 21324 21531 ( 208 n); cDNA 1 208 ( 208 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22575 ( 76 n); cDNA 209 284 ( 76 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E290421+ 1.000 284 1.000 C PGS_C08HBa0251H04.1+_SGN-E290421+ (21324 21531,22500 22575) Alignment (genomic DNA sequence = upper lines): TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 21383 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAAGGCGA TTTTCTTCGA TTCTAAGACA AACCCAGTTC AATTCTGTGT TTAATCTTCC 60 TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTCTAA 21443 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTTCGTCT TTCTCTAAGC CGATCCGAAC AGTTTTACCC AATTCTTTGG TTACCTCTAA 120 GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 21503 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGGTTTTGAG TTGTGGGGTT TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC 180 TCAATCTGTT TATGATTTCA CTGTCAAGGT TAGGACTTTT GCTTATTTTG ATTTGGGGTT 21563 |||||||||| |||||||||| |||||||| TCAATCTGTT TATGATTTCA CTGTCAAG.. .......... .......... .......... 208 TTCCTGAAAT GTTCAATTTT GCTTATTTTG ACTGATTGGT TTAGAAAGAA TGTTGTTTTT 21623 .......... .......... .......... .......... .......... .......... 208 GTTTGTTTTG TTAGTGTTTG GGGTTAGGAA GTTGAGTTAT GTGGTGTGGT TTTTATCATT 21683 .......... .......... .......... .......... .......... .......... 208 TTTTGGGTTC GTGGGCGGTT AAAATTTGGT TTTATATTGG GTATGAATCA GTAACATAAG 21743 .......... .......... .......... .......... .......... .......... 208 CAATGAATGT GAAAGATTAT TTATTTTTGT TTTCTTTTGT GTTAAAGGTT GCTCATTTAT 21803 .......... .......... .......... .......... .......... .......... 208 TAATGCTGTT GTTTAGTTTT TTTGGTTTAT GGTCGTACCT TATGAGTGTG AGCCTTAGAA 21863 .......... .......... .......... .......... .......... .......... 208 ACACGGTAAG GCTGCTTGCA ATAGACTCTT GTGGCTTGGC CCTTCCCTGA GCTGGAGCTT 21923 .......... .......... .......... .......... .......... .......... 208 AGTGCATTGG CTTGCTTTTT TGTCTTTTGC AATTAGAGAT TTGGGGCTTT AAGTTATGAG 21983 .......... .......... .......... .......... .......... .......... 208 GAGTTGTTTT CAACTTATTA GCTCTTGCTT TATTGGTTTA TATAGATTTG GAATGACAAG 22043 .......... .......... .......... .......... .......... .......... 208 ATAAGCATAC AGTGCGAAAT TTCAGTCTTC TTTGTTCACT TCTATCTTTA GGGTGTGAAG 22103 .......... .......... .......... .......... .......... .......... 208 AGAGATGCCT CTGCTGTGTA GTTAGCTGGG TTTAACTTGT TACATGAATG TGGTTGATCT 22163 .......... .......... .......... .......... .......... .......... 208 ACACAAGCAT CTTGTATTGC ATACACTTTC CAACCATCTA AGTGCTATTA GTATAGATGA 22223 .......... .......... .......... .......... .......... .......... 208 TTTTGACTTA TCAGAAAGGA AAATAAAAGA TGAGCAGAGG TGTGAGATTG TGATATATGG 22283 .......... .......... .......... .......... .......... .......... 208 TATAAACTTG GTGCTAGCAA GAAACTAAGG CTTGTTGTTG CATTATTATC TAAACTTGGG 22343 .......... .......... .......... .......... .......... .......... 208 GCTTGTTTTT GTTTCTTTAT TGTCAAAAAG TTTTGTTTTA GGTCTGTTTT GATATTTTCA 22403 .......... .......... .......... .......... .......... .......... 208 ATATAGTATG TTGAGAGTAG TTCTTTTTTG GGACTATACA GTTTGAATAA CCTGAATAAA 22463 .......... .......... .......... .......... .......... .......... 208 ATCTTGGATA ATTGAAGTTC TTTTTATTCT TTATAGGATG CTAAGGGCAA GGACGTTGAT 22523 |||| |||||||||| |||||||||| .......... .......... .......... ......GATG CTAAGGGCAA GGACGTTGAT 232 CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GT 22575 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GT 284 hqPGS_C08HBa0251H04.1+_SGN-E290421+ (21324 21531,22500 22575) ******************************************************************************** EST sequence 43 +strand 558 n (File: SGN-E290457+) 1 TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT 541 GGTATCAAGT GGAACTTC Predicted gene structure (within gDNA segment 20725 to 24404): Exon 1 21325 21531 ( 207 n); cDNA 1 207 ( 207 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 208 284 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 285 346 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 347 465 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23794 ( 93 n); cDNA 466 558 ( 93 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E290457+ 1.000 558 1.000 C PGS_C08HBa0251H04.1+_SGN-E290457+ (21325 21531,22500 22576,23258 23319,23469 23587,23702 23794) Alignment (genomic DNA sequence = upper lines): TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 21384 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 60 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 21444 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 120 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 21504 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 180 CAATCTGTTT ATGATTTCAC TGTCAAGGTT AGGACTTTTG CTTATTTTGA TTTGGGGTTT 21564 |||||||||| |||||||||| ||||||| CAATCTGTTT ATGATTTCAC TGTCAAG... .......... .......... .......... 207 TCCTGAAATG TTCAATTTTG CTTATTTTGA CTGATTGGTT TAGAAAGAAT GTTGTTTTTG 21624 .......... .......... .......... .......... .......... .......... 207 TTTGTTTTGT TAGTGTTTGG GGTTAGGAAG TTGAGTTATG TGGTGTGGTT TTTATCATTT 21684 .......... .......... .......... .......... .......... .......... 207 TTTGGGTTCG TGGGCGGTTA AAATTTGGTT TTATATTGGG TATGAATCAG TAACATAAGC 21744 .......... .......... .......... .......... .......... .......... 207 AATGAATGTG AAAGATTATT TATTTTTGTT TTCTTTTGTG TTAAAGGTTG CTCATTTATT 21804 .......... .......... .......... .......... .......... .......... 207 AATGCTGTTG TTTAGTTTTT TTGGTTTATG GTCGTACCTT ATGAGTGTGA GCCTTAGAAA 21864 .......... .......... .......... .......... .......... .......... 207 CACGGTAAGG CTGCTTGCAA TAGACTCTTG TGGCTTGGCC CTTCCCTGAG CTGGAGCTTA 21924 .......... .......... .......... .......... .......... .......... 207 GTGCATTGGC TTGCTTTTTT GTCTTTTGCA ATTAGAGATT TGGGGCTTTA AGTTATGAGG 21984 .......... .......... .......... .......... .......... .......... 207 AGTTGTTTTC AACTTATTAG CTCTTGCTTT ATTGGTTTAT ATAGATTTGG AATGACAAGA 22044 .......... .......... .......... .......... .......... .......... 207 TAAGCATACA GTGCGAAATT TCAGTCTTCT TTGTTCACTT CTATCTTTAG GGTGTGAAGA 22104 .......... .......... .......... .......... .......... .......... 207 GAGATGCCTC TGCTGTGTAG TTAGCTGGGT TTAACTTGTT ACATGAATGT GGTTGATCTA 22164 .......... .......... .......... .......... .......... .......... 207 CACAAGCATC TTGTATTGCA TACACTTTCC AACCATCTAA GTGCTATTAG TATAGATGAT 22224 .......... .......... .......... .......... .......... .......... 207 TTTGACTTAT CAGAAAGGAA AATAAAAGAT GAGCAGAGGT GTGAGATTGT GATATATGGT 22284 .......... .......... .......... .......... .......... .......... 207 ATAAACTTGG TGCTAGCAAG AAACTAAGGC TTGTTGTTGC ATTATTATCT AAACTTGGGG 22344 .......... .......... .......... .......... .......... .......... 207 CTTGTTTTTG TTTCTTTATT GTCAAAAAGT TTTGTTTTAG GTCTGTTTTG ATATTTTCAA 22404 .......... .......... .......... .......... .......... .......... 207 TATAGTATGT TGAGAGTAGT TCTTTTTTGG GACTATACAG TTTGAATAAC CTGAATAAAA 22464 .......... .......... .......... .......... .......... .......... 207 TCTTGGATAA TTGAAGTTCT TTTTATTCTT TATAGGATGC TAAGGGCAAG GACGTTGATC 22524 ||||| |||||||||| |||||||||| .......... .......... .......... .....GATGC TAAGGGCAAG GACGTTGATC 232 TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT TGCATCACAG TGGTATGTAT 22584 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT TGCATCACAG TG........ 284 TAGTTTCGTA GTTATTTTAT TCATTATATG TTTGAAGCCT CTTCACAATA TTTTGGATTT 22644 .......... .......... .......... .......... .......... .......... 284 CAATAGGAAC TTTCGATGAG AGAAATGATC TGATCGCTAG TTTTTGCTCC TTGTAATTAA 22704 .......... .......... .......... .......... .......... .......... 284 GTGAAATTTT CATGTGTTGC TCTCGATAGA ATTGACTCAA AGTAAAGAAG TATTAAACTT 22764 .......... .......... .......... .......... .......... .......... 284 GAACCTTATG CTACATCAGA TAGGGCATTC CTCCCCGTGA TGAGAATACT ATGCATTGTG 22824 .......... .......... .......... .......... .......... .......... 284 ATAATAACTG AATTTACGAG ATATTTAGTG AAGTTTTCAG GTGTTGTCCT CCATAGAATT 22884 .......... .......... .......... .......... .......... .......... 284 GACTGGAAGT AAAAAAGTAT TAAAATTGAA CCTTATCTTA CACTGGATAG GGCGTTCCTC 22944 .......... .......... .......... .......... .......... .......... 284 CTTGTGATGA AAATACTATG CATTTTTATA AAAACTGAAT CTACGAGAAA TTGAACATGA 23004 .......... .......... .......... .......... .......... .......... 284 CATTATTTTA CTCGAGCCCT GACTAAACAT TCTTACTATG CTTGGTCCAC TACTCAACAA 23064 .......... .......... .......... .......... .......... .......... 284 ACGTGTTCTT AGTACCACTA ATGACAAGTG ATTGTGCATA TCCAGATAAG ATGTCTTTTT 23124 .......... .......... .......... .......... .......... .......... 284 CATTTCGGTA TAATTTGATT TCATTGTGTT TACTGTGAAA GGTTTGAGCA GTTACTTGTT 23184 .......... .......... .......... .......... .......... .......... 284 GTATTTATGA TATCATTCTT TGATCTTGTT ACATTTGCTT ATGACTGTTA ACTGATTCCA 23244 .......... .......... .......... .......... .......... .......... 284 TTTTCTGATG CAGTGGCCTG ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA 23304 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ...TGGCCTG ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA 331 AGTACAAGGA TCAAGGTTTG TCCAATTCTC CCGAACCAAC TGTGCCCTCG ATTCTCCAGC 23364 |||||||||| ||||| AGTACAAGGA TCAAG..... .......... .......... .......... .......... 346 AATGGAGGCG TTCGCCCTGA CATATTTCTG CAAAGAGAAC TTAAATAAAA TGGATGTTTA 23424 .......... .......... .......... .......... .......... .......... 346 TATCTAATTT AGCTTGTTAA TTATATGTTC TTCCTGTTAC ACAGGTTTGG AGATTCTGGC 23484 |||||| |||||||||| .......... .......... .......... .......... ....GTTTGG AGATTCTGGC 362 CTTCCCTTGC AACCAGTTTG GTGGACAGGA GCCTGGAAAC ATTGAAGATA TCCAGCAAAT 23544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCCTTGC AACCAGTTTG GTGGACAGGA GCCTGGAAAC ATTGAAGATA TCCAGCAAAT 422 GGTCTGCACT CGCTTCAAGG CTGAGTACCC CATATTTGAC AAGGTAGAAA TAACACGAAG 23604 |||||||||| |||||||||| |||||||||| |||||||||| ||| GGTCTGCACT CGCTTCAAGG CTGAGTACCC CATATTTGAC AAG....... .......... 465 CATTCTTCTC TTCTAGTCAT CTACACTACA GCTGAAACTG AGAATAAATG AGGCAAGCTG 23664 .......... .......... .......... .......... .......... .......... 465 TATGTACTGA CATCTTCGTG TGTTTTGTGT GGTCTAGGTT GACGTGAATG GTGATAATGC 23724 ||| |||||||||| |||||||||| .......... .......... .......... .......GTT GACGTGAATG GTGATAATGC 488 TGCTCCATTA TATAGGTTTC TGAAATCAAG CAAAGGTGGG TTCTTTGGAG ATGGTATCAA 23784 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTCCATTA TATAGGTTTC TGAAATCAAG CAAAGGTGGG TTCTTTGGAG ATGGTATCAA 548 GTGGAACTTC 23794 |||||||||| GTGGAACTTC 558 hqPGS_C08HBa0251H04.1+_SGN-E290457+ (21325 21531,22500 22576,23258 23319,23469 23587,23702 23794) ******************************************************************************** EST sequence 55 +strand 501 n (File: SGN-E283296+) 1 TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA T Predicted gene structure (within gDNA segment 20725 to 24347): Exon 1 21325 21531 ( 207 n); cDNA 1 207 ( 207 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 208 284 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 285 346 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 347 465 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 0) Exon 5 23702 23737 ( 36 n); cDNA 466 501 ( 36 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E283296+ 1.000 501 1.000 C PGS_C08HBa0251H04.1+_SGN-E283296+ (21325 21531,22500 22576,23258 23319,23469 23587,23702 23737) Alignment (genomic DNA sequence = upper lines): TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 21384 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 60 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 21444 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 120 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 21504 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 180 CAATCTGTTT ATGATTTCAC TGTCAAGGTT AGGACTTTTG CTTATTTTGA TTTGGGGTTT 21564 |||||||||| |||||||||| ||||||| CAATCTGTTT ATGATTTCAC TGTCAAG... .......... .......... .......... 207 TCCTGAAATG TTCAATTTTG CTTATTTTGA CTGATTGGTT TAGAAAGAAT GTTGTTTTTG 21624 .......... .......... .......... .......... .......... .......... 207 TTTGTTTTGT TAGTGTTTGG GGTTAGGAAG TTGAGTTATG TGGTGTGGTT TTTATCATTT 21684 .......... .......... .......... .......... .......... .......... 207 TTTGGGTTCG TGGGCGGTTA AAATTTGGTT TTATATTGGG TATGAATCAG TAACATAAGC 21744 .......... .......... .......... .......... .......... .......... 207 AATGAATGTG AAAGATTATT TATTTTTGTT TTCTTTTGTG TTAAAGGTTG CTCATTTATT 21804 .......... .......... .......... .......... .......... .......... 207 AATGCTGTTG TTTAGTTTTT TTGGTTTATG GTCGTACCTT ATGAGTGTGA GCCTTAGAAA 21864 .......... .......... .......... .......... .......... .......... 207 CACGGTAAGG CTGCTTGCAA TAGACTCTTG TGGCTTGGCC CTTCCCTGAG CTGGAGCTTA 21924 .......... .......... .......... .......... .......... .......... 207 GTGCATTGGC TTGCTTTTTT GTCTTTTGCA ATTAGAGATT TGGGGCTTTA AGTTATGAGG 21984 .......... .......... .......... .......... .......... .......... 207 AGTTGTTTTC AACTTATTAG CTCTTGCTTT ATTGGTTTAT ATAGATTTGG AATGACAAGA 22044 .......... .......... .......... .......... .......... .......... 207 TAAGCATACA GTGCGAAATT TCAGTCTTCT TTGTTCACTT CTATCTTTAG GGTGTGAAGA 22104 .......... .......... .......... .......... .......... .......... 207 GAGATGCCTC TGCTGTGTAG TTAGCTGGGT TTAACTTGTT ACATGAATGT GGTTGATCTA 22164 .......... .......... .......... .......... .......... .......... 207 CACAAGCATC TTGTATTGCA TACACTTTCC AACCATCTAA GTGCTATTAG TATAGATGAT 22224 .......... .......... .......... .......... .......... .......... 207 TTTGACTTAT CAGAAAGGAA AATAAAAGAT GAGCAGAGGT GTGAGATTGT GATATATGGT 22284 .......... .......... .......... .......... .......... .......... 207 ATAAACTTGG TGCTAGCAAG AAACTAAGGC TTGTTGTTGC ATTATTATCT AAACTTGGGG 22344 .......... .......... .......... .......... .......... .......... 207 CTTGTTTTTG TTTCTTTATT GTCAAAAAGT TTTGTTTTAG GTCTGTTTTG ATATTTTCAA 22404 .......... .......... .......... .......... .......... .......... 207 TATAGTATGT TGAGAGTAGT TCTTTTTTGG GACTATACAG TTTGAATAAC CTGAATAAAA 22464 .......... .......... .......... .......... .......... .......... 207 TCTTGGATAA TTGAAGTTCT TTTTATTCTT TATAGGATGC TAAGGGCAAG GACGTTGATC 22524 ||||| |||||||||| |||||||||| .......... .......... .......... .....GATGC TAAGGGCAAG GACGTTGATC 232 TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT TGCATCACAG TGGTATGTAT 22584 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT TGCATCACAG TG........ 284 TAGTTTCGTA GTTATTTTAT TCATTATATG TTTGAAGCCT CTTCACAATA TTTTGGATTT 22644 .......... .......... .......... .......... .......... .......... 284 CAATAGGAAC TTTCGATGAG AGAAATGATC TGATCGCTAG TTTTTGCTCC TTGTAATTAA 22704 .......... .......... .......... .......... .......... .......... 284 GTGAAATTTT CATGTGTTGC TCTCGATAGA ATTGACTCAA AGTAAAGAAG TATTAAACTT 22764 .......... .......... .......... .......... .......... .......... 284 GAACCTTATG CTACATCAGA TAGGGCATTC CTCCCCGTGA TGAGAATACT ATGCATTGTG 22824 .......... .......... .......... .......... .......... .......... 284 ATAATAACTG AATTTACGAG ATATTTAGTG AAGTTTTCAG GTGTTGTCCT CCATAGAATT 22884 .......... .......... .......... .......... .......... .......... 284 GACTGGAAGT AAAAAAGTAT TAAAATTGAA CCTTATCTTA CACTGGATAG GGCGTTCCTC 22944 .......... .......... .......... .......... .......... .......... 284 CTTGTGATGA AAATACTATG CATTTTTATA AAAACTGAAT CTACGAGAAA TTGAACATGA 23004 .......... .......... .......... .......... .......... .......... 284 CATTATTTTA CTCGAGCCCT GACTAAACAT TCTTACTATG CTTGGTCCAC TACTCAACAA 23064 .......... .......... .......... .......... .......... .......... 284 ACGTGTTCTT AGTACCACTA ATGACAAGTG ATTGTGCATA TCCAGATAAG ATGTCTTTTT 23124 .......... .......... .......... .......... .......... .......... 284 CATTTCGGTA TAATTTGATT TCATTGTGTT TACTGTGAAA GGTTTGAGCA GTTACTTGTT 23184 .......... .......... .......... .......... .......... .......... 284 GTATTTATGA TATCATTCTT TGATCTTGTT ACATTTGCTT ATGACTGTTA ACTGATTCCA 23244 .......... .......... .......... .......... .......... .......... 284 TTTTCTGATG CAGTGGCCTG ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA 23304 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ...TGGCCTG ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA 331 AGTACAAGGA TCAAGGTTTG TCCAATTCTC CCGAACCAAC TGTGCCCTCG ATTCTCCAGC 23364 |||||||||| ||||| AGTACAAGGA TCAAG..... .......... .......... .......... .......... 346 AATGGAGGCG TTCGCCCTGA CATATTTCTG CAAAGAGAAC TTAAATAAAA TGGATGTTTA 23424 .......... .......... .......... .......... .......... .......... 346 TATCTAATTT AGCTTGTTAA TTATATGTTC TTCCTGTTAC ACAGGTTTGG AGATTCTGGC 23484 |||||| |||||||||| .......... .......... .......... .......... ....GTTTGG AGATTCTGGC 362 CTTCCCTTGC AACCAGTTTG GTGGACAGGA GCCTGGAAAC ATTGAAGATA TCCAGCAAAT 23544 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTCCCTTGC AACCAGTTTG GTGGACAGGA GCCTGGAAAC ATTGAAGATA TCCAGCAAAT 422 GGTCTGCACT CGCTTCAAGG CTGAGTACCC CATATTTGAC AAGGTAGAAA TAACACGAAG 23604 |||||||||| |||||||||| |||||||||| |||||||||| ||| GGTCTGCACT CGCTTCAAGG CTGAGTACCC CATATTTGAC AAG....... .......... 465 CATTCTTCTC TTCTAGTCAT CTACACTACA GCTGAAACTG AGAATAAATG AGGCAAGCTG 23664 .......... .......... .......... .......... .......... .......... 465 TATGTACTGA CATCTTCGTG TGTTTTGTGT GGTCTAGGTT GACGTGAATG GTGATAATGC 23724 ||| |||||||||| |||||||||| .......... .......... .......... .......GTT GACGTGAATG GTGATAATGC 488 TGCTCCATTA TAT 23737 |||||||||| ||| TGCTCCATTA TAT 501 hqPGS_C08HBa0251H04.1+_SGN-E283296+ (21325 21531,22500 22576,23258 23319,23469 23587,23702 23737) ******************************************************************************** EST sequence 19 +strand 717 n (File: SGN-E390038+) 1 TACGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 61 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 121 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 181 AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT CTCAGTATTT 241 ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG ACTAATTCGA 301 ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG GAGATTCTGG 361 CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA 421 TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC GTGAATGGTG 481 ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC TTTGGAGATG 541 GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC GTTGATCGCT 601 ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC TTGGGTGTTG 661 CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT AGAAATA Predicted gene structure (within gDNA segment 20699 to 24664): Exon 1 21326 21531 ( 206 n); cDNA 1 206 ( 206 n); score: 0.995 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 207 283 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 284 345 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 346 464 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 465 632 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24054 ( 85 n); cDNA 633 717 ( 85 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E390038+ 0.999 717 1.000 C PGS_C08HBa0251H04.1+_SGN-E390038+ (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24054) Alignment (genomic DNA sequence = upper lines): TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 21385 || ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TACGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 60 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 21445 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 120 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 21505 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 180 AATCTGTTTA TGATTTCACT GTCAAGGTTA GGACTTTTGC TTATTTTGAT TTGGGGTTTT 21565 |||||||||| |||||||||| |||||| AATCTGTTTA TGATTTCACT GTCAAG.... .......... .......... .......... 206 CCTGAAATGT TCAATTTTGC TTATTTTGAC TGATTGGTTT AGAAAGAATG TTGTTTTTGT 21625 .......... .......... .......... .......... .......... .......... 206 TTGTTTTGTT AGTGTTTGGG GTTAGGAAGT TGAGTTATGT GGTGTGGTTT TTATCATTTT 21685 .......... .......... .......... .......... .......... .......... 206 TTGGGTTCGT GGGCGGTTAA AATTTGGTTT TATATTGGGT ATGAATCAGT AACATAAGCA 21745 .......... .......... .......... .......... .......... .......... 206 ATGAATGTGA AAGATTATTT ATTTTTGTTT TCTTTTGTGT TAAAGGTTGC TCATTTATTA 21805 .......... .......... .......... .......... .......... .......... 206 ATGCTGTTGT TTAGTTTTTT TGGTTTATGG TCGTACCTTA TGAGTGTGAG CCTTAGAAAC 21865 .......... .......... .......... .......... .......... .......... 206 ACGGTAAGGC TGCTTGCAAT AGACTCTTGT GGCTTGGCCC TTCCCTGAGC TGGAGCTTAG 21925 .......... .......... .......... .......... .......... .......... 206 TGCATTGGCT TGCTTTTTTG TCTTTTGCAA TTAGAGATTT GGGGCTTTAA GTTATGAGGA 21985 .......... .......... .......... .......... .......... .......... 206 GTTGTTTTCA ACTTATTAGC TCTTGCTTTA TTGGTTTATA TAGATTTGGA ATGACAAGAT 22045 .......... .......... .......... .......... .......... .......... 206 AAGCATACAG TGCGAAATTT CAGTCTTCTT TGTTCACTTC TATCTTTAGG GTGTGAAGAG 22105 .......... .......... .......... .......... .......... .......... 206 AGATGCCTCT GCTGTGTAGT TAGCTGGGTT TAACTTGTTA CATGAATGTG GTTGATCTAC 22165 .......... .......... .......... .......... .......... .......... 206 ACAAGCATCT TGTATTGCAT ACACTTTCCA ACCATCTAAG TGCTATTAGT ATAGATGATT 22225 .......... .......... .......... .......... .......... .......... 206 TTGACTTATC AGAAAGGAAA ATAAAAGATG AGCAGAGGTG TGAGATTGTG ATATATGGTA 22285 .......... .......... .......... .......... .......... .......... 206 TAAACTTGGT GCTAGCAAGA AACTAAGGCT TGTTGTTGCA TTATTATCTA AACTTGGGGC 22345 .......... .......... .......... .......... .......... .......... 206 TTGTTTTTGT TTCTTTATTG TCAAAAAGTT TTGTTTTAGG TCTGTTTTGA TATTTTCAAT 22405 .......... .......... .......... .......... .......... .......... 206 ATAGTATGTT GAGAGTAGTT CTTTTTTGGG ACTATACAGT TTGAATAACC TGAATAAAAT 22465 .......... .......... .......... .......... .......... .......... 206 CTTGGATAAT TGAAGTTCTT TTTATTCTTT ATAGGATGCT AAGGGCAAGG ACGTTGATCT 22525 |||||| |||||||||| |||||||||| .......... .......... .......... ....GATGCT AAGGGCAAGG ACGTTGATCT 232 CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT GGTATGTATT 22585 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT G......... 283 AGTTTCGTAG TTATTTTATT CATTATATGT TTGAAGCCTC TTCACAATAT TTTGGATTTC 22645 .......... .......... .......... .......... .......... .......... 283 AATAGGAACT TTCGATGAGA GAAATGATCT GATCGCTAGT TTTTGCTCCT TGTAATTAAG 22705 .......... .......... .......... .......... .......... .......... 283 TGAAATTTTC ATGTGTTGCT CTCGATAGAA TTGACTCAAA GTAAAGAAGT ATTAAACTTG 22765 .......... .......... .......... .......... .......... .......... 283 AACCTTATGC TACATCAGAT AGGGCATTCC TCCCCGTGAT GAGAATACTA TGCATTGTGA 22825 .......... .......... .......... .......... .......... .......... 283 TAATAACTGA ATTTACGAGA TATTTAGTGA AGTTTTCAGG TGTTGTCCTC CATAGAATTG 22885 .......... .......... .......... .......... .......... .......... 283 ACTGGAAGTA AAAAAGTATT AAAATTGAAC CTTATCTTAC ACTGGATAGG GCGTTCCTCC 22945 .......... .......... .......... .......... .......... .......... 283 TTGTGATGAA AATACTATGC ATTTTTATAA AAACTGAATC TACGAGAAAT TGAACATGAC 23005 .......... .......... .......... .......... .......... .......... 283 ATTATTTTAC TCGAGCCCTG ACTAAACATT CTTACTATGC TTGGTCCACT ACTCAACAAA 23065 .......... .......... .......... .......... .......... .......... 283 CGTGTTCTTA GTACCACTAA TGACAAGTGA TTGTGCATAT CCAGATAAGA TGTCTTTTTC 23125 .......... .......... .......... .......... .......... .......... 283 ATTTCGGTAT AATTTGATTT CATTGTGTTT ACTGTGAAAG GTTTGAGCAG TTACTTGTTG 23185 .......... .......... .......... .......... .......... .......... 283 TATTTATGAT ATCATTCTTT GATCTTGTTA CATTTGCTTA TGACTGTTAA CTGATTCCAT 23245 .......... .......... .......... .......... .......... .......... 283 TTTCTGATGC AGTGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 23305 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..TGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 331 GTACAAGGAT CAAGGTTTGT CCAATTCTCC CGAACCAACT GTGCCCTCGA TTCTCCAGCA 23365 |||||||||| |||| GTACAAGGAT CAAG...... .......... .......... .......... .......... 345 ATGGAGGCGT TCGCCCTGAC ATATTTCTGC AAAGAGAACT TAAATAAAAT GGATGTTTAT 23425 .......... .......... .......... .......... .......... .......... 345 ATCTAATTTA GCTTGTTAAT TATATGTTCT TCCTGTTACA CAGGTTTGGA GATTCTGGCC 23485 ||||||| |||||||||| .......... .......... .......... .......... ...GTTTGGA GATTCTGGCC 362 TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT CCAGCAAATG 23545 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT CCAGCAAATG 422 GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AGGTAGAAAT AACACGAAGC 23605 |||||||||| |||||||||| |||||||||| |||||||||| || GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AG........ .......... 464 ATTCTTCTCT TCTAGTCATC TACACTACAG CTGAAACTGA GAATAAATGA GGCAAGCTGT 23665 .......... .......... .......... .......... .......... .......... 464 ATGTACTGAC ATCTTCGTGT GTTTTGTGTG GTCTAGGTTG ACGTGAATGG TGATAATGCT 23725 |||| |||||||||| |||||||||| .......... .......... .......... ......GTTG ACGTGAATGG TGATAATGCT 488 GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA TGGTATCAAG 23785 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA TGGTATCAAG 548 TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG CTACTCTCCA 23845 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG CTACTCTCCA 608 ACCACTTCTC CAGCTAGCAT GGAGGTAAGA ATCCAGTGTC CTGTCCTATG ATAAGCTTCT 23905 |||||||||| |||||||||| |||| ACCACTTCTC CAGCTAGCAT GGAG...... .......... .......... .......... 632 TCACTCAAAA AATTGTTCAC TGACGATATA GCTAATAATA CTCGAACTCT TTGTATTCCT 23965 .......... .......... .......... .......... .......... .......... 632 GCAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG GACTCGGCGG 24025 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....AAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG GACTCGGCGG 688 TGCTGTCTCT GCATTTTGTG CTAGAAATA 24054 |||||||||| |||||||||| ||||||||| TGCTGTCTCT GCATTTTGTG CTAGAAATA 717 hqPGS_C08HBa0251H04.1+_SGN-E390038+ (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24054) ******************************************************************************** EST sequence 22 +strand 710 n (File: SGN-E549946+) 1 TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 61 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 121 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 181 AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT CTCAGTATTT 241 ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG ACTAATTCGA 301 ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG GAGATTCTGG 361 CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA 421 TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC GTGAATGGTG 481 ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC TTTGGAGATG 541 GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC GTTGATCGCT 601 ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC TTGGGTGTTG 661 CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT Predicted gene structure (within gDNA segment 20726 to 24657): Exon 1 21326 21531 ( 206 n); cDNA 1 206 ( 206 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 207 283 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 284 345 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 346 464 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 465 632 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24047 ( 78 n); cDNA 633 710 ( 78 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E549946+ 1.000 710 1.000 C PGS_C08HBa0251H04.1+_SGN-E549946+ (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24047) Alignment (genomic DNA sequence = upper lines): TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 21385 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAGGCGATT TTCTTCGATT CTAAGACAAA CCCAGTTCAA TTCTGTGTTT AATCTTCCTG 60 TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 21445 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCGTCTTT CTCTAAGCCG ATCCGAACAG TTTTACCCAA TTCTTTGGTT ACCTCTAAGG 120 GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 21505 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTGAGTT GTGGGGTTTA AGATCAGATC ATACCATGGC TAGTCAATCA AGCAACCCTC 180 AATCTGTTTA TGATTTCACT GTCAAGGTTA GGACTTTTGC TTATTTTGAT TTGGGGTTTT 21565 |||||||||| |||||||||| |||||| AATCTGTTTA TGATTTCACT GTCAAG.... .......... .......... .......... 206 CCTGAAATGT TCAATTTTGC TTATTTTGAC TGATTGGTTT AGAAAGAATG TTGTTTTTGT 21625 .......... .......... .......... .......... .......... .......... 206 TTGTTTTGTT AGTGTTTGGG GTTAGGAAGT TGAGTTATGT GGTGTGGTTT TTATCATTTT 21685 .......... .......... .......... .......... .......... .......... 206 TTGGGTTCGT GGGCGGTTAA AATTTGGTTT TATATTGGGT ATGAATCAGT AACATAAGCA 21745 .......... .......... .......... .......... .......... .......... 206 ATGAATGTGA AAGATTATTT ATTTTTGTTT TCTTTTGTGT TAAAGGTTGC TCATTTATTA 21805 .......... .......... .......... .......... .......... .......... 206 ATGCTGTTGT TTAGTTTTTT TGGTTTATGG TCGTACCTTA TGAGTGTGAG CCTTAGAAAC 21865 .......... .......... .......... .......... .......... .......... 206 ACGGTAAGGC TGCTTGCAAT AGACTCTTGT GGCTTGGCCC TTCCCTGAGC TGGAGCTTAG 21925 .......... .......... .......... .......... .......... .......... 206 TGCATTGGCT TGCTTTTTTG TCTTTTGCAA TTAGAGATTT GGGGCTTTAA GTTATGAGGA 21985 .......... .......... .......... .......... .......... .......... 206 GTTGTTTTCA ACTTATTAGC TCTTGCTTTA TTGGTTTATA TAGATTTGGA ATGACAAGAT 22045 .......... .......... .......... .......... .......... .......... 206 AAGCATACAG TGCGAAATTT CAGTCTTCTT TGTTCACTTC TATCTTTAGG GTGTGAAGAG 22105 .......... .......... .......... .......... .......... .......... 206 AGATGCCTCT GCTGTGTAGT TAGCTGGGTT TAACTTGTTA CATGAATGTG GTTGATCTAC 22165 .......... .......... .......... .......... .......... .......... 206 ACAAGCATCT TGTATTGCAT ACACTTTCCA ACCATCTAAG TGCTATTAGT ATAGATGATT 22225 .......... .......... .......... .......... .......... .......... 206 TTGACTTATC AGAAAGGAAA ATAAAAGATG AGCAGAGGTG TGAGATTGTG ATATATGGTA 22285 .......... .......... .......... .......... .......... .......... 206 TAAACTTGGT GCTAGCAAGA AACTAAGGCT TGTTGTTGCA TTATTATCTA AACTTGGGGC 22345 .......... .......... .......... .......... .......... .......... 206 TTGTTTTTGT TTCTTTATTG TCAAAAAGTT TTGTTTTAGG TCTGTTTTGA TATTTTCAAT 22405 .......... .......... .......... .......... .......... .......... 206 ATAGTATGTT GAGAGTAGTT CTTTTTTGGG ACTATACAGT TTGAATAACC TGAATAAAAT 22465 .......... .......... .......... .......... .......... .......... 206 CTTGGATAAT TGAAGTTCTT TTTATTCTTT ATAGGATGCT AAGGGCAAGG ACGTTGATCT 22525 |||||| |||||||||| |||||||||| .......... .......... .......... ....GATGCT AAGGGCAAGG ACGTTGATCT 232 CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT GGTATGTATT 22585 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT G......... 283 AGTTTCGTAG TTATTTTATT CATTATATGT TTGAAGCCTC TTCACAATAT TTTGGATTTC 22645 .......... .......... .......... .......... .......... .......... 283 AATAGGAACT TTCGATGAGA GAAATGATCT GATCGCTAGT TTTTGCTCCT TGTAATTAAG 22705 .......... .......... .......... .......... .......... .......... 283 TGAAATTTTC ATGTGTTGCT CTCGATAGAA TTGACTCAAA GTAAAGAAGT ATTAAACTTG 22765 .......... .......... .......... .......... .......... .......... 283 AACCTTATGC TACATCAGAT AGGGCATTCC TCCCCGTGAT GAGAATACTA TGCATTGTGA 22825 .......... .......... .......... .......... .......... .......... 283 TAATAACTGA ATTTACGAGA TATTTAGTGA AGTTTTCAGG TGTTGTCCTC CATAGAATTG 22885 .......... .......... .......... .......... .......... .......... 283 ACTGGAAGTA AAAAAGTATT AAAATTGAAC CTTATCTTAC ACTGGATAGG GCGTTCCTCC 22945 .......... .......... .......... .......... .......... .......... 283 TTGTGATGAA AATACTATGC ATTTTTATAA AAACTGAATC TACGAGAAAT TGAACATGAC 23005 .......... .......... .......... .......... .......... .......... 283 ATTATTTTAC TCGAGCCCTG ACTAAACATT CTTACTATGC TTGGTCCACT ACTCAACAAA 23065 .......... .......... .......... .......... .......... .......... 283 CGTGTTCTTA GTACCACTAA TGACAAGTGA TTGTGCATAT CCAGATAAGA TGTCTTTTTC 23125 .......... .......... .......... .......... .......... .......... 283 ATTTCGGTAT AATTTGATTT CATTGTGTTT ACTGTGAAAG GTTTGAGCAG TTACTTGTTG 23185 .......... .......... .......... .......... .......... .......... 283 TATTTATGAT ATCATTCTTT GATCTTGTTA CATTTGCTTA TGACTGTTAA CTGATTCCAT 23245 .......... .......... .......... .......... .......... .......... 283 TTTCTGATGC AGTGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 23305 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..TGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 331 GTACAAGGAT CAAGGTTTGT CCAATTCTCC CGAACCAACT GTGCCCTCGA TTCTCCAGCA 23365 |||||||||| |||| GTACAAGGAT CAAG...... .......... .......... .......... .......... 345 ATGGAGGCGT TCGCCCTGAC ATATTTCTGC AAAGAGAACT TAAATAAAAT GGATGTTTAT 23425 .......... .......... .......... .......... .......... .......... 345 ATCTAATTTA GCTTGTTAAT TATATGTTCT TCCTGTTACA CAGGTTTGGA GATTCTGGCC 23485 ||||||| |||||||||| .......... .......... .......... .......... ...GTTTGGA GATTCTGGCC 362 TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT CCAGCAAATG 23545 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT CCAGCAAATG 422 GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AGGTAGAAAT AACACGAAGC 23605 |||||||||| |||||||||| |||||||||| |||||||||| || GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AG........ .......... 464 ATTCTTCTCT TCTAGTCATC TACACTACAG CTGAAACTGA GAATAAATGA GGCAAGCTGT 23665 .......... .......... .......... .......... .......... .......... 464 ATGTACTGAC ATCTTCGTGT GTTTTGTGTG GTCTAGGTTG ACGTGAATGG TGATAATGCT 23725 |||| |||||||||| |||||||||| .......... .......... .......... ......GTTG ACGTGAATGG TGATAATGCT 488 GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA TGGTATCAAG 23785 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA TGGTATCAAG 548 TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG CTACTCTCCA 23845 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG CTACTCTCCA 608 ACCACTTCTC CAGCTAGCAT GGAGGTAAGA ATCCAGTGTC CTGTCCTATG ATAAGCTTCT 23905 |||||||||| |||||||||| |||| ACCACTTCTC CAGCTAGCAT GGAG...... .......... .......... .......... 632 TCACTCAAAA AATTGTTCAC TGACGATATA GCTAATAATA CTCGAACTCT TTGTATTCCT 23965 .......... .......... .......... .......... .......... .......... 632 GCAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG GACTCGGCGG 24025 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....AAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG GACTCGGCGG 688 TGCTGTCTCT GCATTTTGTG CT 24047 |||||||||| |||||||||| || TGCTGTCTCT GCATTTTGTG CT 710 hqPGS_C08HBa0251H04.1+_SGN-E549946+ (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24047) ******************************************************************************** EST sequence 35 +strand 647 n (File: SGN-E276053+) 1 AGAAGGCGAT TTTCTTCGAT TCTAAGACAA ACCCAGTTCA ATTCTGTGTT TAATCTTCCT 61 GTTTCGTCTT TCTCTAAGCC GATCCGAACA GTTTTACCCA ATTCTTTGGT TACCTCTAAG 121 GGTTTTGAGT TGTGGGGTTT AAGATCAGAT CATACCATGG CTAGTCAATC AAGCAACCCT 181 CAATCTGTTT ATGATTTCAC TGTCAAGGAT GCTAAGGGCA AGGACGTTGA TCTCAGTATT 241 TACAAGGGAA AAGTCCTTAT CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG 301 AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG 361 GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA 421 ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT 481 GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT 541 GGTATCAAGT GGAACTTCTC CAAATTCCTC ATTGACAAAG AAGGACATGT CGTTGATCGC 601 TACTCTCCAA CCACTTCTCC AGCTAGCATG GAGAAGGATA TCAAGAA Predicted gene structure (within gDNA segment 20707 to 24619): Exon 1 21327 21531 ( 205 n); cDNA 3 207 ( 205 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 208 284 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 285 346 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 347 465 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 466 633 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 0) Exon 6 23970 23983 ( 14 n); cDNA 634 647 ( 14 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E276053+ 1.000 645 0.997 C PGS_C08HBa0251H04.1+_SGN-E276053+ (21327 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 23983) Alignment (genomic DNA sequence = upper lines): AAGGCGATTT TCTTCGATTC TAAGACAAAC CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT 21386 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGCGATTT TCTTCGATTC TAAGACAAAC CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT 62 TTCGTCTTTC TCTAAGCCGA TCCGAACAGT TTTACCCAAT TCTTTGGTTA CCTCTAAGGG 21446 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCGTCTTTC TCTAAGCCGA TCCGAACAGT TTTACCCAAT TCTTTGGTTA CCTCTAAGGG 122 TTTTGAGTTG TGGGGTTTAA GATCAGATCA TACCATGGCT AGTCAATCAA GCAACCCTCA 21506 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTGAGTTG TGGGGTTTAA GATCAGATCA TACCATGGCT AGTCAATCAA GCAACCCTCA 182 ATCTGTTTAT GATTTCACTG TCAAGGTTAG GACTTTTGCT TATTTTGATT TGGGGTTTTC 21566 |||||||||| |||||||||| ||||| ATCTGTTTAT GATTTCACTG TCAAG..... .......... .......... .......... 207 CTGAAATGTT CAATTTTGCT TATTTTGACT GATTGGTTTA GAAAGAATGT TGTTTTTGTT 21626 .......... .......... .......... .......... .......... .......... 207 TGTTTTGTTA GTGTTTGGGG TTAGGAAGTT GAGTTATGTG GTGTGGTTTT TATCATTTTT 21686 .......... .......... .......... .......... .......... .......... 207 TGGGTTCGTG GGCGGTTAAA ATTTGGTTTT ATATTGGGTA TGAATCAGTA ACATAAGCAA 21746 .......... .......... .......... .......... .......... .......... 207 TGAATGTGAA AGATTATTTA TTTTTGTTTT CTTTTGTGTT AAAGGTTGCT CATTTATTAA 21806 .......... .......... .......... .......... .......... .......... 207 TGCTGTTGTT TAGTTTTTTT GGTTTATGGT CGTACCTTAT GAGTGTGAGC CTTAGAAACA 21866 .......... .......... .......... .......... .......... .......... 207 CGGTAAGGCT GCTTGCAATA GACTCTTGTG GCTTGGCCCT TCCCTGAGCT GGAGCTTAGT 21926 .......... .......... .......... .......... .......... .......... 207 GCATTGGCTT GCTTTTTTGT CTTTTGCAAT TAGAGATTTG GGGCTTTAAG TTATGAGGAG 21986 .......... .......... .......... .......... .......... .......... 207 TTGTTTTCAA CTTATTAGCT CTTGCTTTAT TGGTTTATAT AGATTTGGAA TGACAAGATA 22046 .......... .......... .......... .......... .......... .......... 207 AGCATACAGT GCGAAATTTC AGTCTTCTTT GTTCACTTCT ATCTTTAGGG TGTGAAGAGA 22106 .......... .......... .......... .......... .......... .......... 207 GATGCCTCTG CTGTGTAGTT AGCTGGGTTT AACTTGTTAC ATGAATGTGG TTGATCTACA 22166 .......... .......... .......... .......... .......... .......... 207 CAAGCATCTT GTATTGCATA CACTTTCCAA CCATCTAAGT GCTATTAGTA TAGATGATTT 22226 .......... .......... .......... .......... .......... .......... 207 TGACTTATCA GAAAGGAAAA TAAAAGATGA GCAGAGGTGT GAGATTGTGA TATATGGTAT 22286 .......... .......... .......... .......... .......... .......... 207 AAACTTGGTG CTAGCAAGAA ACTAAGGCTT GTTGTTGCAT TATTATCTAA ACTTGGGGCT 22346 .......... .......... .......... .......... .......... .......... 207 TGTTTTTGTT TCTTTATTGT CAAAAAGTTT TGTTTTAGGT CTGTTTTGAT ATTTTCAATA 22406 .......... .......... .......... .......... .......... .......... 207 TAGTATGTTG AGAGTAGTTC TTTTTTGGGA CTATACAGTT TGAATAACCT GAATAAAATC 22466 .......... .......... .......... .......... .......... .......... 207 TTGGATAATT GAAGTTCTTT TTATTCTTTA TAGGATGCTA AGGGCAAGGA CGTTGATCTC 22526 ||||||| |||||||||| |||||||||| .......... .......... .......... ...GATGCTA AGGGCAAGGA CGTTGATCTC 234 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG GTATGTATTA 22586 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG .......... 284 GTTTCGTAGT TATTTTATTC ATTATATGTT TGAAGCCTCT TCACAATATT TTGGATTTCA 22646 .......... .......... .......... .......... .......... .......... 284 ATAGGAACTT TCGATGAGAG AAATGATCTG ATCGCTAGTT TTTGCTCCTT GTAATTAAGT 22706 .......... .......... .......... .......... .......... .......... 284 GAAATTTTCA TGTGTTGCTC TCGATAGAAT TGACTCAAAG TAAAGAAGTA TTAAACTTGA 22766 .......... .......... .......... .......... .......... .......... 284 ACCTTATGCT ACATCAGATA GGGCATTCCT CCCCGTGATG AGAATACTAT GCATTGTGAT 22826 .......... .......... .......... .......... .......... .......... 284 AATAACTGAA TTTACGAGAT ATTTAGTGAA GTTTTCAGGT GTTGTCCTCC ATAGAATTGA 22886 .......... .......... .......... .......... .......... .......... 284 CTGGAAGTAA AAAAGTATTA AAATTGAACC TTATCTTACA CTGGATAGGG CGTTCCTCCT 22946 .......... .......... .......... .......... .......... .......... 284 TGTGATGAAA ATACTATGCA TTTTTATAAA AACTGAATCT ACGAGAAATT GAACATGACA 23006 .......... .......... .......... .......... .......... .......... 284 TTATTTTACT CGAGCCCTGA CTAAACATTC TTACTATGCT TGGTCCACTA CTCAACAAAC 23066 .......... .......... .......... .......... .......... .......... 284 GTGTTCTTAG TACCACTAAT GACAAGTGAT TGTGCATATC CAGATAAGAT GTCTTTTTCA 23126 .......... .......... .......... .......... .......... .......... 284 TTTCGGTATA ATTTGATTTC ATTGTGTTTA CTGTGAAAGG TTTGAGCAGT TACTTGTTGT 23186 .......... .......... .......... .......... .......... .......... 284 ATTTATGATA TCATTCTTTG ATCTTGTTAC ATTTGCTTAT GACTGTTAAC TGATTCCATT 23246 .......... .......... .......... .......... .......... .......... 284 TTCTGATGCA GTGGCCTGAC TAATTCGAAC TACACTGACA TGACGGAGTT ATACAAGAAG 23306 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .TGGCCTGAC TAATTCGAAC TACACTGACA TGACGGAGTT ATACAAGAAG 333 TACAAGGATC AAGGTTTGTC CAATTCTCCC GAACCAACTG TGCCCTCGAT TCTCCAGCAA 23366 |||||||||| ||| TACAAGGATC AAG....... .......... .......... .......... .......... 346 TGGAGGCGTT CGCCCTGACA TATTTCTGCA AAGAGAACTT AAATAAAATG GATGTTTATA 23426 .......... .......... .......... .......... .......... .......... 346 TCTAATTTAG CTTGTTAATT ATATGTTCTT CCTGTTACAC AGGTTTGGAG ATTCTGGCCT 23486 |||||||| |||||||||| .......... .......... .......... .......... ..GTTTGGAG ATTCTGGCCT 364 TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC CAGCAAATGG 23546 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC CAGCAAATGG 424 TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTAGAAATA ACACGAAGCA 23606 |||||||||| |||||||||| |||||||||| |||||||||| | TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA G......... .......... 465 TTCTTCTCTT CTAGTCATCT ACACTACAGC TGAAACTGAG AATAAATGAG GCAAGCTGTA 23666 .......... .......... .......... .......... .......... .......... 465 TGTACTGACA TCTTCGTGTG TTTTGTGTGG TCTAGGTTGA CGTGAATGGT GATAATGCTG 23726 ||||| |||||||||| |||||||||| .......... .......... .......... .....GTTGA CGTGAATGGT GATAATGCTG 490 CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT GGTATCAAGT 23786 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT GGTATCAAGT 550 GGAACTTCTC CAAATTCCTC ATTGACAAAG AAGGACATGT CGTTGATCGC TACTCTCCAA 23846 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAACTTCTC CAAATTCCTC ATTGACAAAG AAGGACATGT CGTTGATCGC TACTCTCCAA 610 CCACTTCTCC AGCTAGCATG GAGGTAAGAA TCCAGTGTCC TGTCCTATGA TAAGCTTCTT 23906 |||||||||| |||||||||| ||| CCACTTCTCC AGCTAGCATG GAG....... .......... .......... .......... 633 CACTCAAAAA ATTGTTCACT GACGATATAG CTAATAATAC TCGAACTCTT TGTATTCCTG 23966 .......... .......... .......... .......... .......... .......... 633 CAGAAGGATA TCAAGAA 23983 ||||||| ||||||| ...AAGGATA TCAAGAA 647 hqPGS_C08HBa0251H04.1+_SGN-E276053+ (21327 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 23983) ******************************************************************************** EST sequence 11 +strand 508 n (File: SGN-E239726+) 1 CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCGTCTTTC TCTAAGCCGA TCCGAACAGT 61 TTTACCCAAT TCTTTGGTTA CCTCTAAGGG TTTTGAGTTG TGGGGTTTAA GATCAGATCA 121 TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG TCAAGGATGC 181 TAAGGGCAAG GACGTTGATC TCAGTATTTA CAAGGGAAAA GTCCTTATCA TTGTCAATGT 241 TGCATCACAG TGTGGGCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT TATACAAGAA 301 GTACAAGGAT CAAGGTTTGG AGATTCTGGC CTTCCCTTGC AACCAGTTTG GTGGACAGGA 361 GCCTGGAAAC ATTGAAGATA TCCAGCAAAT GGTCTGCACT CGCTTCAAGG CTGAGTACCC 421 CATATTTGAC AAGGTTGACG TGAATGGTGA TAATGCTGCT CCATTATATA GGTTTCTGAA 481 ATCAAGCAAA GGTGGGTTCT TTGGAGAT Predicted gene structure (within gDNA segment 20757 to 24386): Exon 1 21357 21531 ( 175 n); cDNA 1 175 ( 175 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 176 252 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 0.98) Exon 3 23258 23319 ( 62 n); cDNA 253 314 ( 62 n); score: 0.984 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 315 433 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23776 ( 75 n); cDNA 434 508 ( 75 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E239726+ 0.998 508 1.000 C PGS_C08HBa0251H04.1+_SGN-E239726+ (21357 21531,22500 22576,23258 23319,23469 23587,23702 23776) Alignment (genomic DNA sequence = upper lines): CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCGTCTTTC TCTAAGCCGA TCCGAACAGT 21416 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCAGTTCAAT TCTGTGTTTA ATCTTCCTGT TTCGTCTTTC TCTAAGCCGA TCCGAACAGT 60 TTTACCCAAT TCTTTGGTTA CCTCTAAGGG TTTTGAGTTG TGGGGTTTAA GATCAGATCA 21476 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTACCCAAT TCTTTGGTTA CCTCTAAGGG TTTTGAGTTG TGGGGTTTAA GATCAGATCA 120 TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG TCAAGGTTAG 21536 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG TCAAG..... 175 GACTTTTGCT TATTTTGATT TGGGGTTTTC CTGAAATGTT CAATTTTGCT TATTTTGACT 21596 .......... .......... .......... .......... .......... .......... 175 GATTGGTTTA GAAAGAATGT TGTTTTTGTT TGTTTTGTTA GTGTTTGGGG TTAGGAAGTT 21656 .......... .......... .......... .......... .......... .......... 175 GAGTTATGTG GTGTGGTTTT TATCATTTTT TGGGTTCGTG GGCGGTTAAA ATTTGGTTTT 21716 .......... .......... .......... .......... .......... .......... 175 ATATTGGGTA TGAATCAGTA ACATAAGCAA TGAATGTGAA AGATTATTTA TTTTTGTTTT 21776 .......... .......... .......... .......... .......... .......... 175 CTTTTGTGTT AAAGGTTGCT CATTTATTAA TGCTGTTGTT TAGTTTTTTT GGTTTATGGT 21836 .......... .......... .......... .......... .......... .......... 175 CGTACCTTAT GAGTGTGAGC CTTAGAAACA CGGTAAGGCT GCTTGCAATA GACTCTTGTG 21896 .......... .......... .......... .......... .......... .......... 175 GCTTGGCCCT TCCCTGAGCT GGAGCTTAGT GCATTGGCTT GCTTTTTTGT CTTTTGCAAT 21956 .......... .......... .......... .......... .......... .......... 175 TAGAGATTTG GGGCTTTAAG TTATGAGGAG TTGTTTTCAA CTTATTAGCT CTTGCTTTAT 22016 .......... .......... .......... .......... .......... .......... 175 TGGTTTATAT AGATTTGGAA TGACAAGATA AGCATACAGT GCGAAATTTC AGTCTTCTTT 22076 .......... .......... .......... .......... .......... .......... 175 GTTCACTTCT ATCTTTAGGG TGTGAAGAGA GATGCCTCTG CTGTGTAGTT AGCTGGGTTT 22136 .......... .......... .......... .......... .......... .......... 175 AACTTGTTAC ATGAATGTGG TTGATCTACA CAAGCATCTT GTATTGCATA CACTTTCCAA 22196 .......... .......... .......... .......... .......... .......... 175 CCATCTAAGT GCTATTAGTA TAGATGATTT TGACTTATCA GAAAGGAAAA TAAAAGATGA 22256 .......... .......... .......... .......... .......... .......... 175 GCAGAGGTGT GAGATTGTGA TATATGGTAT AAACTTGGTG CTAGCAAGAA ACTAAGGCTT 22316 .......... .......... .......... .......... .......... .......... 175 GTTGTTGCAT TATTATCTAA ACTTGGGGCT TGTTTTTGTT TCTTTATTGT CAAAAAGTTT 22376 .......... .......... .......... .......... .......... .......... 175 TGTTTTAGGT CTGTTTTGAT ATTTTCAATA TAGTATGTTG AGAGTAGTTC TTTTTTGGGA 22436 .......... .......... .......... .......... .......... .......... 175 CTATACAGTT TGAATAACCT GAATAAAATC TTGGATAATT GAAGTTCTTT TTATTCTTTA 22496 .......... .......... .......... .......... .......... .......... 175 TAGGATGCTA AGGGCAAGGA CGTTGATCTC AGTATTTACA AGGGAAAAGT CCTTATCATT 22556 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GATGCTA AGGGCAAGGA CGTTGATCTC AGTATTTACA AGGGAAAAGT CCTTATCATT 232 GTCAATGTTG CATCACAGTG GTATGTATTA GTTTCGTAGT TATTTTATTC ATTATATGTT 22616 |||||||||| |||||||||| GTCAATGTTG CATCACAGTG .......... .......... .......... .......... 252 TGAAGCCTCT TCACAATATT TTGGATTTCA ATAGGAACTT TCGATGAGAG AAATGATCTG 22676 .......... .......... .......... .......... .......... .......... 252 ATCGCTAGTT TTTGCTCCTT GTAATTAAGT GAAATTTTCA TGTGTTGCTC TCGATAGAAT 22736 .......... .......... .......... .......... .......... .......... 252 TGACTCAAAG TAAAGAAGTA TTAAACTTGA ACCTTATGCT ACATCAGATA GGGCATTCCT 22796 .......... .......... .......... .......... .......... .......... 252 CCCCGTGATG AGAATACTAT GCATTGTGAT AATAACTGAA TTTACGAGAT ATTTAGTGAA 22856 .......... .......... .......... .......... .......... .......... 252 GTTTTCAGGT GTTGTCCTCC ATAGAATTGA CTGGAAGTAA AAAAGTATTA AAATTGAACC 22916 .......... .......... .......... .......... .......... .......... 252 TTATCTTACA CTGGATAGGG CGTTCCTCCT TGTGATGAAA ATACTATGCA TTTTTATAAA 22976 .......... .......... .......... .......... .......... .......... 252 AACTGAATCT ACGAGAAATT GAACATGACA TTATTTTACT CGAGCCCTGA CTAAACATTC 23036 .......... .......... .......... .......... .......... .......... 252 TTACTATGCT TGGTCCACTA CTCAACAAAC GTGTTCTTAG TACCACTAAT GACAAGTGAT 23096 .......... .......... .......... .......... .......... .......... 252 TGTGCATATC CAGATAAGAT GTCTTTTTCA TTTCGGTATA ATTTGATTTC ATTGTGTTTA 23156 .......... .......... .......... .......... .......... .......... 252 CTGTGAAAGG TTTGAGCAGT TACTTGTTGT ATTTATGATA TCATTCTTTG ATCTTGTTAC 23216 .......... .......... .......... .......... .......... .......... 252 ATTTGCTTAT GACTGTTAAC TGATTCCATT TTCTGATGCA GTGGCCTGAC TAATTCGAAC 23276 ||| ||||| |||||||||| .......... .......... .......... .......... .TGGGCTGAC TAATTCGAAC 271 TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAGGTTTGTC CAATTCTCCC 23336 |||||||||| |||||||||| |||||||||| |||||||||| ||| TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAG....... .......... 314 GAACCAACTG TGCCCTCGAT TCTCCAGCAA TGGAGGCGTT CGCCCTGACA TATTTCTGCA 23396 .......... .......... .......... .......... .......... .......... 314 AAGAGAACTT AAATAAAATG GATGTTTATA TCTAATTTAG CTTGTTAATT ATATGTTCTT 23456 .......... .......... .......... .......... .......... .......... 314 CCTGTTACAC AGGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC 23516 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..GTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC 362 CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA 23576 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA 422 TATTTGACAA GGTAGAAATA ACACGAAGCA TTCTTCTCTT CTAGTCATCT ACACTACAGC 23636 |||||||||| | TATTTGACAA G......... .......... .......... .......... .......... 433 TGAAACTGAG AATAAATGAG GCAAGCTGTA TGTACTGACA TCTTCGTGTG TTTTGTGTGG 23696 .......... .......... .......... .......... .......... .......... 433 TCTAGGTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA 23756 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....GTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA 488 AAGGTGGGTT CTTTGGAGAT 23776 |||||||||| |||||||||| AAGGTGGGTT CTTTGGAGAT 508 hqPGS_C08HBa0251H04.1+_SGN-E239726+ (21357 21531,22500 22576,23258 23319,23469 23587,23702 23776) ******************************************************************************** EST sequence 42 +strand 287 n (File: SGN-E290519+) 1 TAAGATCAGA TCATACCATG GCTAGTGAAT CAAGCAACCC TCAATCTGTT ATGATTTCAC 61 TGGCAAGGAT GCTAAGGGCA AGGACCTTGA TCTCAGAATT TACAAGGGAA AAGGCCTTAT 121 CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG AACTACACTG ACATGACGGC 181 GATATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG GCCTTACCTT GCAACCAGTT 241 TGGGGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA ATGGTCT Predicted gene structure (within gDNA segment 20864 to 24159): Exon 1 21464 21531 ( 68 n); cDNA 1 67 ( 67 n); score: 0.956 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0.94), Pa: 0.999 (s: 0.94) Exon 2 22500 22576 ( 77 n); cDNA 68 144 ( 77 n); score: 0.961 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 0.96), Pa: 1.000 (s: 0.96) Exon 3 23258 23319 ( 62 n); cDNA 145 206 ( 62 n); score: 0.968 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 0.96), Pa: 1.000 (s: 0.96) Exon 4 23469 23549 ( 81 n); cDNA 207 287 ( 81 n); score: 0.975 MATCH C08HBa0251H04.1+ SGN-E290519+ 0.965 288 1.003 C PGS_C08HBa0251H04.1+_SGN-E290519+ (21464 21531,22500 22576,23258 23319,23469 23549) Alignment (genomic DNA sequence = upper lines): TAAGATCAGA TCATACCATG GCTAGTCAAT CAAGCAACCC TCAATCTGTT TATGATTTCA 21523 |||||||||| |||||||||| |||||| ||| |||||||||| |||||||| | |||||||||| TAAGATCAGA TCATACCATG GCTAGTGAAT CAAGCAACCC TCAATCTG-T TATGATTTCA 59 CTGTCAAGGT TAGGACTTTT GCTTATTTTG ATTTGGGGTT TTCCTGAAAT GTTCAATTTT 21583 ||| |||| CTGGCAAG.. .......... .......... .......... .......... .......... 67 GCTTATTTTG ACTGATTGGT TTAGAAAGAA TGTTGTTTTT GTTTGTTTTG TTAGTGTTTG 21643 .......... .......... .......... .......... .......... .......... 67 GGGTTAGGAA GTTGAGTTAT GTGGTGTGGT TTTTATCATT TTTTGGGTTC GTGGGCGGTT 21703 .......... .......... .......... .......... .......... .......... 67 AAAATTTGGT TTTATATTGG GTATGAATCA GTAACATAAG CAATGAATGT GAAAGATTAT 21763 .......... .......... .......... .......... .......... .......... 67 TTATTTTTGT TTTCTTTTGT GTTAAAGGTT GCTCATTTAT TAATGCTGTT GTTTAGTTTT 21823 .......... .......... .......... .......... .......... .......... 67 TTTGGTTTAT GGTCGTACCT TATGAGTGTG AGCCTTAGAA ACACGGTAAG GCTGCTTGCA 21883 .......... .......... .......... .......... .......... .......... 67 ATAGACTCTT GTGGCTTGGC CCTTCCCTGA GCTGGAGCTT AGTGCATTGG CTTGCTTTTT 21943 .......... .......... .......... .......... .......... .......... 67 TGTCTTTTGC AATTAGAGAT TTGGGGCTTT AAGTTATGAG GAGTTGTTTT CAACTTATTA 22003 .......... .......... .......... .......... .......... .......... 67 GCTCTTGCTT TATTGGTTTA TATAGATTTG GAATGACAAG ATAAGCATAC AGTGCGAAAT 22063 .......... .......... .......... .......... .......... .......... 67 TTCAGTCTTC TTTGTTCACT TCTATCTTTA GGGTGTGAAG AGAGATGCCT CTGCTGTGTA 22123 .......... .......... .......... .......... .......... .......... 67 GTTAGCTGGG TTTAACTTGT TACATGAATG TGGTTGATCT ACACAAGCAT CTTGTATTGC 22183 .......... .......... .......... .......... .......... .......... 67 ATACACTTTC CAACCATCTA AGTGCTATTA GTATAGATGA TTTTGACTTA TCAGAAAGGA 22243 .......... .......... .......... .......... .......... .......... 67 AAATAAAAGA TGAGCAGAGG TGTGAGATTG TGATATATGG TATAAACTTG GTGCTAGCAA 22303 .......... .......... .......... .......... .......... .......... 67 GAAACTAAGG CTTGTTGTTG CATTATTATC TAAACTTGGG GCTTGTTTTT GTTTCTTTAT 22363 .......... .......... .......... .......... .......... .......... 67 TGTCAAAAAG TTTTGTTTTA GGTCTGTTTT GATATTTTCA ATATAGTATG TTGAGAGTAG 22423 .......... .......... .......... .......... .......... .......... 67 TTCTTTTTTG GGACTATACA GTTTGAATAA CCTGAATAAA ATCTTGGATA ATTGAAGTTC 22483 .......... .......... .......... .......... .......... .......... 67 TTTTTATTCT TTATAGGATG CTAAGGGCAA GGACGTTGAT CTCAGTATTT ACAAGGGAAA 22543 |||| |||||||||| |||| ||||| ||||| |||| |||||||||| .......... ......GATG CTAAGGGCAA GGACCTTGAT CTCAGAATTT ACAAGGGAAA 111 AGTCCTTATC ATTGTCAATG TTGCATCACA GTGGTATGTA TTAGTTTCGT AGTTATTTTA 22603 || ||||||| |||||||||| |||||||||| ||| AGGCCTTATC ATTGTCAATG TTGCATCACA GTG....... .......... .......... 144 TTCATTATAT GTTTGAAGCC TCTTCACAAT ATTTTGGATT TCAATAGGAA CTTTCGATGA 22663 .......... .......... .......... .......... .......... .......... 144 GAGAAATGAT CTGATCGCTA GTTTTTGCTC CTTGTAATTA AGTGAAATTT TCATGTGTTG 22723 .......... .......... .......... .......... .......... .......... 144 CTCTCGATAG AATTGACTCA AAGTAAAGAA GTATTAAACT TGAACCTTAT GCTACATCAG 22783 .......... .......... .......... .......... .......... .......... 144 ATAGGGCATT CCTCCCCGTG ATGAGAATAC TATGCATTGT GATAATAACT GAATTTACGA 22843 .......... .......... .......... .......... .......... .......... 144 GATATTTAGT GAAGTTTTCA GGTGTTGTCC TCCATAGAAT TGACTGGAAG TAAAAAAGTA 22903 .......... .......... .......... .......... .......... .......... 144 TTAAAATTGA ACCTTATCTT ACACTGGATA GGGCGTTCCT CCTTGTGATG AAAATACTAT 22963 .......... .......... .......... .......... .......... .......... 144 GCATTTTTAT AAAAACTGAA TCTACGAGAA ATTGAACATG ACATTATTTT ACTCGAGCCC 23023 .......... .......... .......... .......... .......... .......... 144 TGACTAAACA TTCTTACTAT GCTTGGTCCA CTACTCAACA AACGTGTTCT TAGTACCACT 23083 .......... .......... .......... .......... .......... .......... 144 AATGACAAGT GATTGTGCAT ATCCAGATAA GATGTCTTTT TCATTTCGGT ATAATTTGAT 23143 .......... .......... .......... .......... .......... .......... 144 TTCATTGTGT TTACTGTGAA AGGTTTGAGC AGTTACTTGT TGTATTTATG ATATCATTCT 23203 .......... .......... .......... .......... .......... .......... 144 TTGATCTTGT TACATTTGCT TATGACTGTT AACTGATTCC ATTTTCTGAT GCAGTGGCCT 23263 |||||| .......... .......... .......... .......... .......... ....TGGCCT 150 GACTAATTCG AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT 23323 |||||||||| |||||||||| ||||||||| | |||||||| |||||||||| |||||| GACTAATTCG AACTACACTG ACATGACGGC GATATACAAG AAGTACAAGG ATCAAG.... 206 GTCCAATTCT CCCGAACCAA CTGTGCCCTC GATTCTCCAG CAATGGAGGC GTTCGCCCTG 23383 .......... .......... .......... .......... .......... .......... 206 ACATATTTCT GCAAAGAGAA CTTAAATAAA ATGGATGTTT ATATCTAATT TAGCTTGTTA 23443 .......... .......... .......... .......... .......... .......... 206 ATTATATGTT CTTCCTGTTA CACAGGTTTG GAGATTCTGG CCTTCCCTTG CAACCAGTTT 23503 ||||| |||||||||| |||| ||||| |||||||||| .......... .......... .....GTTTG GAGATTCTGG CCTTACCTTG CAACCAGTTT 241 GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA TGGTCT 23549 || ||||||| |||||||||| |||||||||| |||||||||| |||||| GGGGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA TGGTCT 287 hqPGS_C08HBa0251H04.1+_SGN-E290519+ (21464 21531,22500 22576,23258 23319,23469 23549) ******************************************************************************** EST sequence 91 -strand 644 n (File: SGN-E550257-) 1 GATCAGATCA TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG 61 TCAAGGATGC TAAGGGCAAG GACGTTGATC TCAGTATTTA CAAGGGAAAA GTCCTTATCA 121 TTGTCAATGT TGCATCACAG TGTGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT 181 TATACAAGAA GTACAAGGAT CAAGGTTTGG AGATTCTGGC CTTCCCTTGC AACCAGTTTG 241 GTGGACAGGA GCCTGGAAAC ATTGAAGATA TCCAGCAAAT GGTCTGCACT CGCTTCAAGG 301 CTGAGTACCC CATATTTGAC AAGGTTGACG TGAATGGTGA TAATGCTGCT CCATTATATA 361 GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG TATCAAGTGG AACTTCTCCA 421 AATTCCTCAT TGACAAAGAA GGACATGTCG TTGATCGCTA CTCTCCAACC ACTTCTCCAG 481 CTAGCATGGA GAAGGATATC AAGAAACTCT TGGGTGTTGC TTAAGCTCTA GAGTCTGGAC 541 TCGGCGGTGC TGTCTCTGCA TTTTGTGCTA GAAATAATGA ACATATCATT GCGAATAAGC 601 TATGTTTGAT GTAATTTTGT TCTTGTTTAG TTCCTACAGA TGTT Predicted gene structure (within gDNA segment 20857 to 24723): Exon 1 21467 21531 ( 65 n); cDNA 1 65 ( 65 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 66 142 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 143 204 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 205 323 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 324 491 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24123 ( 154 n); cDNA 492 644 ( 153 n); score: 0.987 MATCH C08HBa0251H04.1+ SGN-E550257- 0.997 645 1.002 C PGS_C08HBa0251H04.1+_SGN-E550257- (21467 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24123) Alignment (genomic DNA sequence = upper lines): GATCAGATCA TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG 21526 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATCAGATCA TACCATGGCT AGTCAATCAA GCAACCCTCA ATCTGTTTAT GATTTCACTG 60 TCAAGGTTAG GACTTTTGCT TATTTTGATT TGGGGTTTTC CTGAAATGTT CAATTTTGCT 21586 ||||| TCAAG..... .......... .......... .......... .......... .......... 65 TATTTTGACT GATTGGTTTA GAAAGAATGT TGTTTTTGTT TGTTTTGTTA GTGTTTGGGG 21646 .......... .......... .......... .......... .......... .......... 65 TTAGGAAGTT GAGTTATGTG GTGTGGTTTT TATCATTTTT TGGGTTCGTG GGCGGTTAAA 21706 .......... .......... .......... .......... .......... .......... 65 ATTTGGTTTT ATATTGGGTA TGAATCAGTA ACATAAGCAA TGAATGTGAA AGATTATTTA 21766 .......... .......... .......... .......... .......... .......... 65 TTTTTGTTTT CTTTTGTGTT AAAGGTTGCT CATTTATTAA TGCTGTTGTT TAGTTTTTTT 21826 .......... .......... .......... .......... .......... .......... 65 GGTTTATGGT CGTACCTTAT GAGTGTGAGC CTTAGAAACA CGGTAAGGCT GCTTGCAATA 21886 .......... .......... .......... .......... .......... .......... 65 GACTCTTGTG GCTTGGCCCT TCCCTGAGCT GGAGCTTAGT GCATTGGCTT GCTTTTTTGT 21946 .......... .......... .......... .......... .......... .......... 65 CTTTTGCAAT TAGAGATTTG GGGCTTTAAG TTATGAGGAG TTGTTTTCAA CTTATTAGCT 22006 .......... .......... .......... .......... .......... .......... 65 CTTGCTTTAT TGGTTTATAT AGATTTGGAA TGACAAGATA AGCATACAGT GCGAAATTTC 22066 .......... .......... .......... .......... .......... .......... 65 AGTCTTCTTT GTTCACTTCT ATCTTTAGGG TGTGAAGAGA GATGCCTCTG CTGTGTAGTT 22126 .......... .......... .......... .......... .......... .......... 65 AGCTGGGTTT AACTTGTTAC ATGAATGTGG TTGATCTACA CAAGCATCTT GTATTGCATA 22186 .......... .......... .......... .......... .......... .......... 65 CACTTTCCAA CCATCTAAGT GCTATTAGTA TAGATGATTT TGACTTATCA GAAAGGAAAA 22246 .......... .......... .......... .......... .......... .......... 65 TAAAAGATGA GCAGAGGTGT GAGATTGTGA TATATGGTAT AAACTTGGTG CTAGCAAGAA 22306 .......... .......... .......... .......... .......... .......... 65 ACTAAGGCTT GTTGTTGCAT TATTATCTAA ACTTGGGGCT TGTTTTTGTT TCTTTATTGT 22366 .......... .......... .......... .......... .......... .......... 65 CAAAAAGTTT TGTTTTAGGT CTGTTTTGAT ATTTTCAATA TAGTATGTTG AGAGTAGTTC 22426 .......... .......... .......... .......... .......... .......... 65 TTTTTTGGGA CTATACAGTT TGAATAACCT GAATAAAATC TTGGATAATT GAAGTTCTTT 22486 .......... .......... .......... .......... .......... .......... 65 TTATTCTTTA TAGGATGCTA AGGGCAAGGA CGTTGATCTC AGTATTTACA AGGGAAAAGT 22546 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ...GATGCTA AGGGCAAGGA CGTTGATCTC AGTATTTACA AGGGAAAAGT 112 CCTTATCATT GTCAATGTTG CATCACAGTG GTATGTATTA GTTTCGTAGT TATTTTATTC 22606 |||||||||| |||||||||| |||||||||| CCTTATCATT GTCAATGTTG CATCACAGTG .......... .......... .......... 142 ATTATATGTT TGAAGCCTCT TCACAATATT TTGGATTTCA ATAGGAACTT TCGATGAGAG 22666 .......... .......... .......... .......... .......... .......... 142 AAATGATCTG ATCGCTAGTT TTTGCTCCTT GTAATTAAGT GAAATTTTCA TGTGTTGCTC 22726 .......... .......... .......... .......... .......... .......... 142 TCGATAGAAT TGACTCAAAG TAAAGAAGTA TTAAACTTGA ACCTTATGCT ACATCAGATA 22786 .......... .......... .......... .......... .......... .......... 142 GGGCATTCCT CCCCGTGATG AGAATACTAT GCATTGTGAT AATAACTGAA TTTACGAGAT 22846 .......... .......... .......... .......... .......... .......... 142 ATTTAGTGAA GTTTTCAGGT GTTGTCCTCC ATAGAATTGA CTGGAAGTAA AAAAGTATTA 22906 .......... .......... .......... .......... .......... .......... 142 AAATTGAACC TTATCTTACA CTGGATAGGG CGTTCCTCCT TGTGATGAAA ATACTATGCA 22966 .......... .......... .......... .......... .......... .......... 142 TTTTTATAAA AACTGAATCT ACGAGAAATT GAACATGACA TTATTTTACT CGAGCCCTGA 23026 .......... .......... .......... .......... .......... .......... 142 CTAAACATTC TTACTATGCT TGGTCCACTA CTCAACAAAC GTGTTCTTAG TACCACTAAT 23086 .......... .......... .......... .......... .......... .......... 142 GACAAGTGAT TGTGCATATC CAGATAAGAT GTCTTTTTCA TTTCGGTATA ATTTGATTTC 23146 .......... .......... .......... .......... .......... .......... 142 ATTGTGTTTA CTGTGAAAGG TTTGAGCAGT TACTTGTTGT ATTTATGATA TCATTCTTTG 23206 .......... .......... .......... .......... .......... .......... 142 ATCTTGTTAC ATTTGCTTAT GACTGTTAAC TGATTCCATT TTCTGATGCA GTGGCCTGAC 23266 ||||||||| .......... .......... .......... .......... .......... .TGGCCTGAC 151 TAATTCGAAC TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAGGTTTGTC 23326 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| TAATTCGAAC TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAG....... 204 CAATTCTCCC GAACCAACTG TGCCCTCGAT TCTCCAGCAA TGGAGGCGTT CGCCCTGACA 23386 .......... .......... .......... .......... .......... .......... 204 TATTTCTGCA AAGAGAACTT AAATAAAATG GATGTTTATA TCTAATTTAG CTTGTTAATT 23446 .......... .......... .......... .......... .......... .......... 204 ATATGTTCTT CCTGTTACAC AGGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT 23506 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..GTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT 242 GGACAGGAGC CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT 23566 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGACAGGAGC CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT 302 GAGTACCCCA TATTTGACAA GGTAGAAATA ACACGAAGCA TTCTTCTCTT CTAGTCATCT 23626 |||||||||| |||||||||| | GAGTACCCCA TATTTGACAA G......... .......... .......... .......... 323 ACACTACAGC TGAAACTGAG AATAAATGAG GCAAGCTGTA TGTACTGACA TCTTCGTGTG 23686 .......... .......... .......... .......... .......... .......... 323 TTTTGTGTGG TCTAGGTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG 23746 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG 368 AAATCAAGCA AAGGTGGGTT CTTTGGAGAT GGTATCAAGT GGAACTTCTC CAAATTCCTC 23806 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAATCAAGCA AAGGTGGGTT CTTTGGAGAT GGTATCAAGT GGAACTTCTC CAAATTCCTC 428 ATTGACAAAG AAGGACATGT CGTTGATCGC TACTCTCCAA CCACTTCTCC AGCTAGCATG 23866 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGACAAAG AAGGACATGT CGTTGATCGC TACTCTCCAA CCACTTCTCC AGCTAGCATG 488 GAGGTAAGAA TCCAGTGTCC TGTCCTATGA TAAGCTTCTT CACTCAAAAA ATTGTTCACT 23926 ||| GAG....... .......... .......... .......... .......... .......... 491 GACGATATAG CTAATAATAC TCGAACTCTT TGTATTCCTG CAGAAGGATA TCAAGAAACT 23986 ||||||| |||||||||| .......... .......... .......... .......... ...AAGGATA TCAAGAAACT 508 CTTGGGTGTT GCTTAAGCTC TAGAGTCTGG ACTCGGCGGT GCTGTCTCTG CATTTTGTGC 24046 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGGGTGTT GCTTAAGCTC TAGAGTCTGG ACTCGGCGGT GCTGTCTCTG CATTTTGTGC 568 TAGAAATAAT GAACATATCA TTGCGAATAA GCTATGTTTG ATGTAATTTT CTTCTTGTTT 24106 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| || TAGAAATAAT GAACATATCA TTGCGAATAA GCTATGTTTG ATGTAATTTT GTTCTTG-TT 627 TAGTTCCTAC AGATGTT 24123 |||||||||| ||||||| TAGTTCCTAC AGATGTT 644 hqPGS_C08HBa0251H04.1+_SGN-E550257- (21467 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24123) ******************************************************************************** EST sequence 135 -strand 754 n (File: SGN-E396338-) 1 AGTCAATCAA GCAACCCTCA ATCTGTTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA 61 GGACGTTGAT CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TGNCATCACA 121 GTGTGGCCTG ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA 181 TCAAGGTTTG GAGATTCTGG CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA 241 CATTGAAGAT ATCCAGCAAA TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA 301 CAAGGTTGAC GTGAATGGTG ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA 361 AGGTGGGTTC TTTGGAGATG GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA 421 AGGACATGTC GTTGATCGCT ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT 481 CAAGAAACTC TTGGGTGTTG CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC 541 ATTTTGTGCT AGAAATAATG AACATATCAT TGCGAATAAG CTATGTTTGA TGTAATTTTC 601 TTCTTGTTTT AGTTCCTACA GATGTTTGCT ACTTTGTACA ATTTCAATAT ACTGATCAAT 661 ATTTCTATTT ACAAGTATTA TCAAGTTTTT TCCAACTTTT GGTAAAAAGA TTGCTAGTAA 721 AGATAAATAA CACTTCATCA TTTCAAAAAA AAAA Predicted gene structure (within gDNA segment 20877 to 24914): Exon 1 21487 21531 ( 45 n); cDNA 1 46 ( 46 n); score: 0.944 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0.94), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 47 123 ( 77 n); score: 0.974 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 0.96), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 124 185 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 186 304 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 305 472 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24244 ( 275 n); cDNA 473 747 ( 275 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E396338- 0.997 746 0.989 C PGS_C08HBa0251H04.1+_SGN-E396338- (21487 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24244) Alignment (genomic DNA sequence = upper lines): AGTCAATCAA GCAACCCTCA ATCTG-TTTA TGATTTCACT GTCAAGGTTA GGACTTTTGC 21545 |||||||||| |||||||||| ||||| |||| |||||||||| |||||| AGTCAATCAA GCAACCCTCA ATCTGTTTTA TGATTTCACT GTCAAG.... .......... 46 TTATTTTGAT TTGGGGTTTT CCTGAAATGT TCAATTTTGC TTATTTTGAC TGATTGGTTT 21605 .......... .......... .......... .......... .......... .......... 46 AGAAAGAATG TTGTTTTTGT TTGTTTTGTT AGTGTTTGGG GTTAGGAAGT TGAGTTATGT 21665 .......... .......... .......... .......... .......... .......... 46 GGTGTGGTTT TTATCATTTT TTGGGTTCGT GGGCGGTTAA AATTTGGTTT TATATTGGGT 21725 .......... .......... .......... .......... .......... .......... 46 ATGAATCAGT AACATAAGCA ATGAATGTGA AAGATTATTT ATTTTTGTTT TCTTTTGTGT 21785 .......... .......... .......... .......... .......... .......... 46 TAAAGGTTGC TCATTTATTA ATGCTGTTGT TTAGTTTTTT TGGTTTATGG TCGTACCTTA 21845 .......... .......... .......... .......... .......... .......... 46 TGAGTGTGAG CCTTAGAAAC ACGGTAAGGC TGCTTGCAAT AGACTCTTGT GGCTTGGCCC 21905 .......... .......... .......... .......... .......... .......... 46 TTCCCTGAGC TGGAGCTTAG TGCATTGGCT TGCTTTTTTG TCTTTTGCAA TTAGAGATTT 21965 .......... .......... .......... .......... .......... .......... 46 GGGGCTTTAA GTTATGAGGA GTTGTTTTCA ACTTATTAGC TCTTGCTTTA TTGGTTTATA 22025 .......... .......... .......... .......... .......... .......... 46 TAGATTTGGA ATGACAAGAT AAGCATACAG TGCGAAATTT CAGTCTTCTT TGTTCACTTC 22085 .......... .......... .......... .......... .......... .......... 46 TATCTTTAGG GTGTGAAGAG AGATGCCTCT GCTGTGTAGT TAGCTGGGTT TAACTTGTTA 22145 .......... .......... .......... .......... .......... .......... 46 CATGAATGTG GTTGATCTAC ACAAGCATCT TGTATTGCAT ACACTTTCCA ACCATCTAAG 22205 .......... .......... .......... .......... .......... .......... 46 TGCTATTAGT ATAGATGATT TTGACTTATC AGAAAGGAAA ATAAAAGATG AGCAGAGGTG 22265 .......... .......... .......... .......... .......... .......... 46 TGAGATTGTG ATATATGGTA TAAACTTGGT GCTAGCAAGA AACTAAGGCT TGTTGTTGCA 22325 .......... .......... .......... .......... .......... .......... 46 TTATTATCTA AACTTGGGGC TTGTTTTTGT TTCTTTATTG TCAAAAAGTT TTGTTTTAGG 22385 .......... .......... .......... .......... .......... .......... 46 TCTGTTTTGA TATTTTCAAT ATAGTATGTT GAGAGTAGTT CTTTTTTGGG ACTATACAGT 22445 .......... .......... .......... .......... .......... .......... 46 TTGAATAACC TGAATAAAAT CTTGGATAAT TGAAGTTCTT TTTATTCTTT ATAGGATGCT 22505 |||||| .......... .......... .......... .......... .......... ....GATGCT 52 AAGGGCAAGG ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT 22565 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AAGGGCAAGG ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTG 112 GCATCACAGT GGTATGTATT AGTTTCGTAG TTATTTTATT CATTATATGT TTGAAGCCTC 22625 ||||||||| | NCATCACAGT G......... .......... .......... .......... .......... 123 TTCACAATAT TTTGGATTTC AATAGGAACT TTCGATGAGA GAAATGATCT GATCGCTAGT 22685 .......... .......... .......... .......... .......... .......... 123 TTTTGCTCCT TGTAATTAAG TGAAATTTTC ATGTGTTGCT CTCGATAGAA TTGACTCAAA 22745 .......... .......... .......... .......... .......... .......... 123 GTAAAGAAGT ATTAAACTTG AACCTTATGC TACATCAGAT AGGGCATTCC TCCCCGTGAT 22805 .......... .......... .......... .......... .......... .......... 123 GAGAATACTA TGCATTGTGA TAATAACTGA ATTTACGAGA TATTTAGTGA AGTTTTCAGG 22865 .......... .......... .......... .......... .......... .......... 123 TGTTGTCCTC CATAGAATTG ACTGGAAGTA AAAAAGTATT AAAATTGAAC CTTATCTTAC 22925 .......... .......... .......... .......... .......... .......... 123 ACTGGATAGG GCGTTCCTCC TTGTGATGAA AATACTATGC ATTTTTATAA AAACTGAATC 22985 .......... .......... .......... .......... .......... .......... 123 TACGAGAAAT TGAACATGAC ATTATTTTAC TCGAGCCCTG ACTAAACATT CTTACTATGC 23045 .......... .......... .......... .......... .......... .......... 123 TTGGTCCACT ACTCAACAAA CGTGTTCTTA GTACCACTAA TGACAAGTGA TTGTGCATAT 23105 .......... .......... .......... .......... .......... .......... 123 CCAGATAAGA TGTCTTTTTC ATTTCGGTAT AATTTGATTT CATTGTGTTT ACTGTGAAAG 23165 .......... .......... .......... .......... .......... .......... 123 GTTTGAGCAG TTACTTGTTG TATTTATGAT ATCATTCTTT GATCTTGTTA CATTTGCTTA 23225 .......... .......... .......... .......... .......... .......... 123 TGACTGTTAA CTGATTCCAT TTTCTGATGC AGTGGCCTGA CTAATTCGAA CTACACTGAC 23285 |||||||| |||||||||| |||||||||| .......... .......... .......... ..TGGCCTGA CTAATTCGAA CTACACTGAC 151 ATGACGGAGT TATACAAGAA GTACAAGGAT CAAGGTTTGT CCAATTCTCC CGAACCAACT 23345 |||||||||| |||||||||| |||||||||| |||| ATGACGGAGT TATACAAGAA GTACAAGGAT CAAG...... .......... .......... 185 GTGCCCTCGA TTCTCCAGCA ATGGAGGCGT TCGCCCTGAC ATATTTCTGC AAAGAGAACT 23405 .......... .......... .......... .......... .......... .......... 185 TAAATAAAAT GGATGTTTAT ATCTAATTTA GCTTGTTAAT TATATGTTCT TCCTGTTACA 23465 .......... .......... .......... .......... .......... .......... 185 CAGGTTTGGA GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA 23525 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GTTTGGA GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA 242 TTGAAGATAT CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA 23585 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGAAGATAT CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA 302 AGGTAGAAAT AACACGAAGC ATTCTTCTCT TCTAGTCATC TACACTACAG CTGAAACTGA 23645 || AG........ .......... .......... .......... .......... .......... 304 GAATAAATGA GGCAAGCTGT ATGTACTGAC ATCTTCGTGT GTTTTGTGTG GTCTAGGTTG 23705 |||| .......... .......... .......... .......... .......... ......GTTG 308 ACGTGAATGG TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT 23765 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTGAATGG TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT 368 TCTTTGGAGA TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG 23825 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTTGGAGA TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG 428 TCGTTGATCG CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAGGTAAGA ATCCAGTGTC 23885 |||||||||| |||||||||| |||||||||| |||||||||| |||| TCGTTGATCG CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAG...... .......... 472 CTGTCCTATG ATAAGCTTCT TCACTCAAAA AATTGTTCAC TGACGATATA GCTAATAATA 23945 .......... .......... .......... .......... .......... .......... 472 CTCGAACTCT TTGTATTCCT GCAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT 24005 |||||| |||||||||| |||||||||| |||||||||| .......... .......... ....AAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT 508 CTAGAGTCTG GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TGAACATATC 24065 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTAGAGTCTG GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TGAACATATC 568 ATTGCGAATA AGCTATGTTT GATGTAATTT TCTTCTTGTT TTAGTTCCTA CAGATGTTTG 24125 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGCGAATA AGCTATGTTT GATGTAATTT TCTTCTTGTT TTAGTTCCTA CAGATGTTTG 628 CTACTTTGTA CAATTTCAAT ATACTGATCA ATATTTCTAT TTACAAGTAT TATCAAGTTT 24185 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTACTTTGTA CAATTTCAAT ATACTGATCA ATATTTCTAT TTACAAGTAT TATCAAGTTT 688 TTTCCAACTT TTGGTAAAAA GATTGCTAGT AAAGATAAAT AACACTTCAT CATTTCAAA 24244 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TTTCCAACTT TTGGTAAAAA GATTGCTAGT AAAGATAAAT AACACTTCAT CATTTCAAA 747 hqPGS_C08HBa0251H04.1+_SGN-E396338- (21487 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24244) ******************************************************************************** EST sequence 37 +strand 647 n (File: SGN-E274781+) 1 AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT 61 CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG 121 ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG 181 GAGATTCTGG CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT 241 ATCCAGCAAA TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC 301 GTGAATGGTG ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC 361 TTTGGAGATG GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC 421 GTTGATCGCT ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC 481 TTGGGTGTTG CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT 541 AGAAATAATG AACATATCAT TGCGAATAAG CTATGTTTGA TGTAATTTTC TTCTTGTTTT 601 AGTTCCTACA GATGTTTGCT ACTTTGTACA ATTTCAATAT ACTGATC Predicted gene structure (within gDNA segment 20896 to 24764): Exon 1 21496 21531 ( 36 n); cDNA 1 36 ( 36 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 37 113 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 114 175 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 176 294 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 295 462 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24154 ( 185 n); cDNA 463 647 ( 185 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E274781+ 1.000 647 1.000 C PGS_C08HBa0251H04.1+_SGN-E274781+ (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24154) Alignment (genomic DNA sequence = upper lines): AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAGGTTA GGACTTTTGC TTATTTTGAT 21555 |||||||||| |||||||||| |||||||||| |||||| AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAG.... .......... .......... 36 TTGGGGTTTT CCTGAAATGT TCAATTTTGC TTATTTTGAC TGATTGGTTT AGAAAGAATG 21615 .......... .......... .......... .......... .......... .......... 36 TTGTTTTTGT TTGTTTTGTT AGTGTTTGGG GTTAGGAAGT TGAGTTATGT GGTGTGGTTT 21675 .......... .......... .......... .......... .......... .......... 36 TTATCATTTT TTGGGTTCGT GGGCGGTTAA AATTTGGTTT TATATTGGGT ATGAATCAGT 21735 .......... .......... .......... .......... .......... .......... 36 AACATAAGCA ATGAATGTGA AAGATTATTT ATTTTTGTTT TCTTTTGTGT TAAAGGTTGC 21795 .......... .......... .......... .......... .......... .......... 36 TCATTTATTA ATGCTGTTGT TTAGTTTTTT TGGTTTATGG TCGTACCTTA TGAGTGTGAG 21855 .......... .......... .......... .......... .......... .......... 36 CCTTAGAAAC ACGGTAAGGC TGCTTGCAAT AGACTCTTGT GGCTTGGCCC TTCCCTGAGC 21915 .......... .......... .......... .......... .......... .......... 36 TGGAGCTTAG TGCATTGGCT TGCTTTTTTG TCTTTTGCAA TTAGAGATTT GGGGCTTTAA 21975 .......... .......... .......... .......... .......... .......... 36 GTTATGAGGA GTTGTTTTCA ACTTATTAGC TCTTGCTTTA TTGGTTTATA TAGATTTGGA 22035 .......... .......... .......... .......... .......... .......... 36 ATGACAAGAT AAGCATACAG TGCGAAATTT CAGTCTTCTT TGTTCACTTC TATCTTTAGG 22095 .......... .......... .......... .......... .......... .......... 36 GTGTGAAGAG AGATGCCTCT GCTGTGTAGT TAGCTGGGTT TAACTTGTTA CATGAATGTG 22155 .......... .......... .......... .......... .......... .......... 36 GTTGATCTAC ACAAGCATCT TGTATTGCAT ACACTTTCCA ACCATCTAAG TGCTATTAGT 22215 .......... .......... .......... .......... .......... .......... 36 ATAGATGATT TTGACTTATC AGAAAGGAAA ATAAAAGATG AGCAGAGGTG TGAGATTGTG 22275 .......... .......... .......... .......... .......... .......... 36 ATATATGGTA TAAACTTGGT GCTAGCAAGA AACTAAGGCT TGTTGTTGCA TTATTATCTA 22335 .......... .......... .......... .......... .......... .......... 36 AACTTGGGGC TTGTTTTTGT TTCTTTATTG TCAAAAAGTT TTGTTTTAGG TCTGTTTTGA 22395 .......... .......... .......... .......... .......... .......... 36 TATTTTCAAT ATAGTATGTT GAGAGTAGTT CTTTTTTGGG ACTATACAGT TTGAATAACC 22455 .......... .......... .......... .......... .......... .......... 36 TGAATAAAAT CTTGGATAAT TGAAGTTCTT TTTATTCTTT ATAGGATGCT AAGGGCAAGG 22515 |||||| |||||||||| .......... .......... .......... .......... ....GATGCT AAGGGCAAGG 52 ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT 22575 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT 112 GGTATGTATT AGTTTCGTAG TTATTTTATT CATTATATGT TTGAAGCCTC TTCACAATAT 22635 | G......... .......... .......... .......... .......... .......... 113 TTTGGATTTC AATAGGAACT TTCGATGAGA GAAATGATCT GATCGCTAGT TTTTGCTCCT 22695 .......... .......... .......... .......... .......... .......... 113 TGTAATTAAG TGAAATTTTC ATGTGTTGCT CTCGATAGAA TTGACTCAAA GTAAAGAAGT 22755 .......... .......... .......... .......... .......... .......... 113 ATTAAACTTG AACCTTATGC TACATCAGAT AGGGCATTCC TCCCCGTGAT GAGAATACTA 22815 .......... .......... .......... .......... .......... .......... 113 TGCATTGTGA TAATAACTGA ATTTACGAGA TATTTAGTGA AGTTTTCAGG TGTTGTCCTC 22875 .......... .......... .......... .......... .......... .......... 113 CATAGAATTG ACTGGAAGTA AAAAAGTATT AAAATTGAAC CTTATCTTAC ACTGGATAGG 22935 .......... .......... .......... .......... .......... .......... 113 GCGTTCCTCC TTGTGATGAA AATACTATGC ATTTTTATAA AAACTGAATC TACGAGAAAT 22995 .......... .......... .......... .......... .......... .......... 113 TGAACATGAC ATTATTTTAC TCGAGCCCTG ACTAAACATT CTTACTATGC TTGGTCCACT 23055 .......... .......... .......... .......... .......... .......... 113 ACTCAACAAA CGTGTTCTTA GTACCACTAA TGACAAGTGA TTGTGCATAT CCAGATAAGA 23115 .......... .......... .......... .......... .......... .......... 113 TGTCTTTTTC ATTTCGGTAT AATTTGATTT CATTGTGTTT ACTGTGAAAG GTTTGAGCAG 23175 .......... .......... .......... .......... .......... .......... 113 TTACTTGTTG TATTTATGAT ATCATTCTTT GATCTTGTTA CATTTGCTTA TGACTGTTAA 23235 .......... .......... .......... .......... .......... .......... 113 CTGATTCCAT TTTCTGATGC AGTGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT 23295 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..TGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT 151 TATACAAGAA GTACAAGGAT CAAGGTTTGT CCAATTCTCC CGAACCAACT GTGCCCTCGA 23355 |||||||||| |||||||||| |||| TATACAAGAA GTACAAGGAT CAAG...... .......... .......... .......... 175 TTCTCCAGCA ATGGAGGCGT TCGCCCTGAC ATATTTCTGC AAAGAGAACT TAAATAAAAT 23415 .......... .......... .......... .......... .......... .......... 175 GGATGTTTAT ATCTAATTTA GCTTGTTAAT TATATGTTCT TCCTGTTACA CAGGTTTGGA 23475 ||||||| .......... .......... .......... .......... .......... ...GTTTGGA 182 GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT 23535 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT 242 CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AGGTAGAAAT 23595 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AG........ 294 AACACGAAGC ATTCTTCTCT TCTAGTCATC TACACTACAG CTGAAACTGA GAATAAATGA 23655 .......... .......... .......... .......... .......... .......... 294 GGCAAGCTGT ATGTACTGAC ATCTTCGTGT GTTTTGTGTG GTCTAGGTTG ACGTGAATGG 23715 |||| |||||||||| .......... .......... .......... .......... ......GTTG ACGTGAATGG 308 TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA 23775 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA 368 TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG 23835 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG 428 CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAGGTAAGA ATCCAGTGTC CTGTCCTATG 23895 |||||||||| |||||||||| |||||||||| |||| CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAG...... .......... .......... 462 ATAAGCTTCT TCACTCAAAA AATTGTTCAC TGACGATATA GCTAATAATA CTCGAACTCT 23955 .......... .......... .......... .......... .......... .......... 462 TTGTATTCCT GCAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG 24015 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....AAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG 508 GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TGAACATATC ATTGCGAATA 24075 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TGAACATATC ATTGCGAATA 568 AGCTATGTTT GATGTAATTT TCTTCTTGTT TTAGTTCCTA CAGATGTTTG CTACTTTGTA 24135 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTATGTTT GATGTAATTT TCTTCTTGTT TTAGTTCCTA CAGATGTTTG CTACTTTGTA 628 CAATTTCAAT ATACTGATC 24154 |||||||||| ||||||||| CAATTTCAAT ATACTGATC 647 hqPGS_C08HBa0251H04.1+_SGN-E274781+ (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24154) ******************************************************************************** EST sequence 38 +strand 550 n (File: SGN-E276673+) 1 AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAGGATG CTAAGGGCAA GGACGTTGAT 61 CTCAGTATTT ACAAGGGAAA AGTCCTTATC ATTGTCAATG TTGCATCACA GTGTGGCCTG 121 ACTAATTCGA ACTACACTGA CATGACGGAG TTATACAAGA AGTACAAGGA TCAAGGTTTG 181 GAGATTCTGG CCTTCCCTTG CAACCAGTTT GGTGGACAGG AGCCTGGAAA CATTGAAGAT 241 ATCCAGCAAA TGGTCTGCAC TCGCTTCAAG GCTGAGTACC CCATATTTGA CAAGGTTGAC 301 GTGAATGGTG ATAATGCTGC TCCATTATAT AGGTTTCTGA AATCAAGCAA AGGTGGGTTC 361 TTTGGAGATG GTATCAAGTG GAACTTCTCC AAATTCCTCA TTGACAAAGA AGGACATGTC 421 GTTGATCGCT ACTCTCCAAC CACTTCTCCA GCTAGCATGG AGAAGGATAT CAAGAAACTC 481 TTGGGTGTTG CTTAAGCTCT AGAGTCTGGA CTCGGCGGTG CTGTCTCTGC ATTTTGTGCT 541 AGAAATAATG Predicted gene structure (within gDNA segment 20896 to 24667): Exon 1 21496 21531 ( 36 n); cDNA 1 36 ( 36 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 37 113 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 114 175 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 176 294 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 295 462 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 6 23970 24057 ( 88 n); cDNA 463 550 ( 88 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E276673+ 1.000 550 1.000 C PGS_C08HBa0251H04.1+_SGN-E276673+ (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24057) Alignment (genomic DNA sequence = upper lines): AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAGGTTA GGACTTTTGC TTATTTTGAT 21555 |||||||||| |||||||||| |||||||||| |||||| AGCAACCCTC AATCTGTTTA TGATTTCACT GTCAAG.... .......... .......... 36 TTGGGGTTTT CCTGAAATGT TCAATTTTGC TTATTTTGAC TGATTGGTTT AGAAAGAATG 21615 .......... .......... .......... .......... .......... .......... 36 TTGTTTTTGT TTGTTTTGTT AGTGTTTGGG GTTAGGAAGT TGAGTTATGT GGTGTGGTTT 21675 .......... .......... .......... .......... .......... .......... 36 TTATCATTTT TTGGGTTCGT GGGCGGTTAA AATTTGGTTT TATATTGGGT ATGAATCAGT 21735 .......... .......... .......... .......... .......... .......... 36 AACATAAGCA ATGAATGTGA AAGATTATTT ATTTTTGTTT TCTTTTGTGT TAAAGGTTGC 21795 .......... .......... .......... .......... .......... .......... 36 TCATTTATTA ATGCTGTTGT TTAGTTTTTT TGGTTTATGG TCGTACCTTA TGAGTGTGAG 21855 .......... .......... .......... .......... .......... .......... 36 CCTTAGAAAC ACGGTAAGGC TGCTTGCAAT AGACTCTTGT GGCTTGGCCC TTCCCTGAGC 21915 .......... .......... .......... .......... .......... .......... 36 TGGAGCTTAG TGCATTGGCT TGCTTTTTTG TCTTTTGCAA TTAGAGATTT GGGGCTTTAA 21975 .......... .......... .......... .......... .......... .......... 36 GTTATGAGGA GTTGTTTTCA ACTTATTAGC TCTTGCTTTA TTGGTTTATA TAGATTTGGA 22035 .......... .......... .......... .......... .......... .......... 36 ATGACAAGAT AAGCATACAG TGCGAAATTT CAGTCTTCTT TGTTCACTTC TATCTTTAGG 22095 .......... .......... .......... .......... .......... .......... 36 GTGTGAAGAG AGATGCCTCT GCTGTGTAGT TAGCTGGGTT TAACTTGTTA CATGAATGTG 22155 .......... .......... .......... .......... .......... .......... 36 GTTGATCTAC ACAAGCATCT TGTATTGCAT ACACTTTCCA ACCATCTAAG TGCTATTAGT 22215 .......... .......... .......... .......... .......... .......... 36 ATAGATGATT TTGACTTATC AGAAAGGAAA ATAAAAGATG AGCAGAGGTG TGAGATTGTG 22275 .......... .......... .......... .......... .......... .......... 36 ATATATGGTA TAAACTTGGT GCTAGCAAGA AACTAAGGCT TGTTGTTGCA TTATTATCTA 22335 .......... .......... .......... .......... .......... .......... 36 AACTTGGGGC TTGTTTTTGT TTCTTTATTG TCAAAAAGTT TTGTTTTAGG TCTGTTTTGA 22395 .......... .......... .......... .......... .......... .......... 36 TATTTTCAAT ATAGTATGTT GAGAGTAGTT CTTTTTTGGG ACTATACAGT TTGAATAACC 22455 .......... .......... .......... .......... .......... .......... 36 TGAATAAAAT CTTGGATAAT TGAAGTTCTT TTTATTCTTT ATAGGATGCT AAGGGCAAGG 22515 |||||| |||||||||| .......... .......... .......... .......... ....GATGCT AAGGGCAAGG 52 ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT 22575 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTTGATCT CAGTATTTAC AAGGGAAAAG TCCTTATCAT TGTCAATGTT GCATCACAGT 112 GGTATGTATT AGTTTCGTAG TTATTTTATT CATTATATGT TTGAAGCCTC TTCACAATAT 22635 | G......... .......... .......... .......... .......... .......... 113 TTTGGATTTC AATAGGAACT TTCGATGAGA GAAATGATCT GATCGCTAGT TTTTGCTCCT 22695 .......... .......... .......... .......... .......... .......... 113 TGTAATTAAG TGAAATTTTC ATGTGTTGCT CTCGATAGAA TTGACTCAAA GTAAAGAAGT 22755 .......... .......... .......... .......... .......... .......... 113 ATTAAACTTG AACCTTATGC TACATCAGAT AGGGCATTCC TCCCCGTGAT GAGAATACTA 22815 .......... .......... .......... .......... .......... .......... 113 TGCATTGTGA TAATAACTGA ATTTACGAGA TATTTAGTGA AGTTTTCAGG TGTTGTCCTC 22875 .......... .......... .......... .......... .......... .......... 113 CATAGAATTG ACTGGAAGTA AAAAAGTATT AAAATTGAAC CTTATCTTAC ACTGGATAGG 22935 .......... .......... .......... .......... .......... .......... 113 GCGTTCCTCC TTGTGATGAA AATACTATGC ATTTTTATAA AAACTGAATC TACGAGAAAT 22995 .......... .......... .......... .......... .......... .......... 113 TGAACATGAC ATTATTTTAC TCGAGCCCTG ACTAAACATT CTTACTATGC TTGGTCCACT 23055 .......... .......... .......... .......... .......... .......... 113 ACTCAACAAA CGTGTTCTTA GTACCACTAA TGACAAGTGA TTGTGCATAT CCAGATAAGA 23115 .......... .......... .......... .......... .......... .......... 113 TGTCTTTTTC ATTTCGGTAT AATTTGATTT CATTGTGTTT ACTGTGAAAG GTTTGAGCAG 23175 .......... .......... .......... .......... .......... .......... 113 TTACTTGTTG TATTTATGAT ATCATTCTTT GATCTTGTTA CATTTGCTTA TGACTGTTAA 23235 .......... .......... .......... .......... .......... .......... 113 CTGATTCCAT TTTCTGATGC AGTGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT 23295 |||||||| |||||||||| |||||||||| |||||||||| .......... .......... ..TGGCCTGA CTAATTCGAA CTACACTGAC ATGACGGAGT 151 TATACAAGAA GTACAAGGAT CAAGGTTTGT CCAATTCTCC CGAACCAACT GTGCCCTCGA 23355 |||||||||| |||||||||| |||| TATACAAGAA GTACAAGGAT CAAG...... .......... .......... .......... 175 TTCTCCAGCA ATGGAGGCGT TCGCCCTGAC ATATTTCTGC AAAGAGAACT TAAATAAAAT 23415 .......... .......... .......... .......... .......... .......... 175 GGATGTTTAT ATCTAATTTA GCTTGTTAAT TATATGTTCT TCCTGTTACA CAGGTTTGGA 23475 ||||||| .......... .......... .......... .......... .......... ...GTTTGGA 182 GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT 23535 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATTCTGGCC TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT 242 CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AGGTAGAAAT 23595 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || CCAGCAAATG GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AG........ 294 AACACGAAGC ATTCTTCTCT TCTAGTCATC TACACTACAG CTGAAACTGA GAATAAATGA 23655 .......... .......... .......... .......... .......... .......... 294 GGCAAGCTGT ATGTACTGAC ATCTTCGTGT GTTTTGTGTG GTCTAGGTTG ACGTGAATGG 23715 |||| |||||||||| .......... .......... .......... .......... ......GTTG ACGTGAATGG 308 TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA 23775 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA 368 TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG 23835 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG 428 CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAGGTAAGA ATCCAGTGTC CTGTCCTATG 23895 |||||||||| |||||||||| |||||||||| |||| CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAG...... .......... .......... 462 ATAAGCTTCT TCACTCAAAA AATTGTTCAC TGACGATATA GCTAATAATA CTCGAACTCT 23955 .......... .......... .......... .......... .......... .......... 462 TTGTATTCCT GCAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG 24015 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....AAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG 508 GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TG 24057 |||||||||| |||||||||| |||||||||| |||||||||| || GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TG 550 hqPGS_C08HBa0251H04.1+_SGN-E276673+ (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24057) ******************************************************************************** EST sequence 48 +strand 581 n (File: SGN-E276156+) 1 AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGATGCTA AGGGCAAGGA CGTTGATCTC 61 AGTATTTACA AGGGAAAAGT CCTTATCATT GTCAATGTTG CATCACAGTG TGGCCTGACT 121 AATTCGAACT ACACTGACAT GACGGAGTTA TACAAGAAGT ACAAGGATCA AGGTTTGGAG 181 ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC CTGGAAACAT TGAAGATATC 241 CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA TATTTGACAA GGTTGACGTG 301 AATGGTGATA ATGCTGCTCC ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT 361 GGAGATGGTA TCAAGTGGAA CTTCTCCAAA TTCCTCATTG ACAAAGAAGG ACATGTCGTT 421 GATCGCTACT CTCCAACCAC TTGTCCAGCT AGCATGGAGA AGGATATCAA GAAACTCTTG 481 GGTGTTGCTT AAGCTCTAGA GTCTGGACTC GGCGGTGCTG TCTCTGCATT TTGTGCTAGA 541 AATAATGAAC ATATCATTGC GAATAAGCTA TGTTTGATGT A Predicted gene structure (within gDNA segment 20899 to 24701): Exon 1 21499 21531 ( 33 n); cDNA 1 33 ( 33 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 (s: 0), Pa: 0.999 (s: 1.00) Exon 2 22500 22576 ( 77 n); cDNA 34 110 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23258 23319 ( 62 n); cDNA 111 172 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 4 23469 23587 ( 119 n); cDNA 173 291 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 5 23702 23869 ( 168 n); cDNA 292 459 ( 168 n); score: 0.994 Intron 5 23870 23969 ( 100 n); Pd: 1.000 (s: 0.98), Pa: 0.984 (s: 1.00) Exon 6 23970 24091 ( 122 n); cDNA 460 581 ( 122 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E276156+ 0.998 581 1.000 C PGS_C08HBa0251H04.1+_SGN-E276156+ (21499 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24091) Alignment (genomic DNA sequence = upper lines): AACCCTCAAT CTGTTTATGA TTTCACTGTC AAGGTTAGGA CTTTTGCTTA TTTTGATTTG 21558 |||||||||| |||||||||| |||||||||| ||| AACCCTCAAT CTGTTTATGA TTTCACTGTC AAG....... .......... .......... 33 GGGTTTTCCT GAAATGTTCA ATTTTGCTTA TTTTGACTGA TTGGTTTAGA AAGAATGTTG 21618 .......... .......... .......... .......... .......... .......... 33 TTTTTGTTTG TTTTGTTAGT GTTTGGGGTT AGGAAGTTGA GTTATGTGGT GTGGTTTTTA 21678 .......... .......... .......... .......... .......... .......... 33 TCATTTTTTG GGTTCGTGGG CGGTTAAAAT TTGGTTTTAT ATTGGGTATG AATCAGTAAC 21738 .......... .......... .......... .......... .......... .......... 33 ATAAGCAATG AATGTGAAAG ATTATTTATT TTTGTTTTCT TTTGTGTTAA AGGTTGCTCA 21798 .......... .......... .......... .......... .......... .......... 33 TTTATTAATG CTGTTGTTTA GTTTTTTTGG TTTATGGTCG TACCTTATGA GTGTGAGCCT 21858 .......... .......... .......... .......... .......... .......... 33 TAGAAACACG GTAAGGCTGC TTGCAATAGA CTCTTGTGGC TTGGCCCTTC CCTGAGCTGG 21918 .......... .......... .......... .......... .......... .......... 33 AGCTTAGTGC ATTGGCTTGC TTTTTTGTCT TTTGCAATTA GAGATTTGGG GCTTTAAGTT 21978 .......... .......... .......... .......... .......... .......... 33 ATGAGGAGTT GTTTTCAACT TATTAGCTCT TGCTTTATTG GTTTATATAG ATTTGGAATG 22038 .......... .......... .......... .......... .......... .......... 33 ACAAGATAAG CATACAGTGC GAAATTTCAG TCTTCTTTGT TCACTTCTAT CTTTAGGGTG 22098 .......... .......... .......... .......... .......... .......... 33 TGAAGAGAGA TGCCTCTGCT GTGTAGTTAG CTGGGTTTAA CTTGTTACAT GAATGTGGTT 22158 .......... .......... .......... .......... .......... .......... 33 GATCTACACA AGCATCTTGT ATTGCATACA CTTTCCAACC ATCTAAGTGC TATTAGTATA 22218 .......... .......... .......... .......... .......... .......... 33 GATGATTTTG ACTTATCAGA AAGGAAAATA AAAGATGAGC AGAGGTGTGA GATTGTGATA 22278 .......... .......... .......... .......... .......... .......... 33 TATGGTATAA ACTTGGTGCT AGCAAGAAAC TAAGGCTTGT TGTTGCATTA TTATCTAAAC 22338 .......... .......... .......... .......... .......... .......... 33 TTGGGGCTTG TTTTTGTTTC TTTATTGTCA AAAAGTTTTG TTTTAGGTCT GTTTTGATAT 22398 .......... .......... .......... .......... .......... .......... 33 TTTCAATATA GTATGTTGAG AGTAGTTCTT TTTTGGGACT ATACAGTTTG AATAACCTGA 22458 .......... .......... .......... .......... .......... .......... 33 ATAAAATCTT GGATAATTGA AGTTCTTTTT ATTCTTTATA GGATGCTAAG GGCAAGGACG 22518 ||||||||| |||||||||| .......... .......... .......... .......... .GATGCTAAG GGCAAGGACG 52 TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTGGT 22578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TTGATCTCAG TATTTACAAG GGAAAAGTCC TTATCATTGT CAATGTTGCA TCACAGTG.. 110 ATGTATTAGT TTCGTAGTTA TTTTATTCAT TATATGTTTG AAGCCTCTTC ACAATATTTT 22638 .......... .......... .......... .......... .......... .......... 110 GGATTTCAAT AGGAACTTTC GATGAGAGAA ATGATCTGAT CGCTAGTTTT TGCTCCTTGT 22698 .......... .......... .......... .......... .......... .......... 110 AATTAAGTGA AATTTTCATG TGTTGCTCTC GATAGAATTG ACTCAAAGTA AAGAAGTATT 22758 .......... .......... .......... .......... .......... .......... 110 AAACTTGAAC CTTATGCTAC ATCAGATAGG GCATTCCTCC CCGTGATGAG AATACTATGC 22818 .......... .......... .......... .......... .......... .......... 110 ATTGTGATAA TAACTGAATT TACGAGATAT TTAGTGAAGT TTTCAGGTGT TGTCCTCCAT 22878 .......... .......... .......... .......... .......... .......... 110 AGAATTGACT GGAAGTAAAA AAGTATTAAA ATTGAACCTT ATCTTACACT GGATAGGGCG 22938 .......... .......... .......... .......... .......... .......... 110 TTCCTCCTTG TGATGAAAAT ACTATGCATT TTTATAAAAA CTGAATCTAC GAGAAATTGA 22998 .......... .......... .......... .......... .......... .......... 110 ACATGACATT ATTTTACTCG AGCCCTGACT AAACATTCTT ACTATGCTTG GTCCACTACT 23058 .......... .......... .......... .......... .......... .......... 110 CAACAAACGT GTTCTTAGTA CCACTAATGA CAAGTGATTG TGCATATCCA GATAAGATGT 23118 .......... .......... .......... .......... .......... .......... 110 CTTTTTCATT TCGGTATAAT TTGATTTCAT TGTGTTTACT GTGAAAGGTT TGAGCAGTTA 23178 .......... .......... .......... .......... .......... .......... 110 CTTGTTGTAT TTATGATATC ATTCTTTGAT CTTGTTACAT TTGCTTATGA CTGTTAACTG 23238 .......... .......... .......... .......... .......... .......... 110 ATTCCATTTT CTGATGCAGT GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 23298 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........T GGCCTGACTA ATTCGAACTA CACTGACATG ACGGAGTTAT 151 ACAAGAAGTA CAAGGATCAA GGTTTGTCCA ATTCTCCCGA ACCAACTGTG CCCTCGATTC 23358 |||||||||| |||||||||| | ACAAGAAGTA CAAGGATCAA G......... .......... .......... .......... 172 TCCAGCAATG GAGGCGTTCG CCCTGACATA TTTCTGCAAA GAGAACTTAA ATAAAATGGA 23418 .......... .......... .......... .......... .......... .......... 172 TGTTTATATC TAATTTAGCT TGTTAATTAT ATGTTCTTCC TGTTACACAG GTTTGGAGAT 23478 |||||||||| .......... .......... .......... .......... .......... GTTTGGAGAT 182 TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 23538 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT GGAAACATTG AAGATATCCA 242 GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAGG TAGAAATAAC 23598 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA TTTGACAAG. .......... 291 ACGAAGCATT CTTCTCTTCT AGTCATCTAC ACTACAGCTG AAACTGAGAA TAAATGAGGC 23658 .......... .......... .......... .......... .......... .......... 291 AAGCTGTATG TACTGACATC TTCGTGTGTT TTGTGTGGTC TAGGTTGACG TGAATGGTGA 23718 ||||||| |||||||||| .......... .......... .......... .......... ...GTTGACG TGAATGGTGA 308 TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 23778 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATGCTGCT CCATTATATA GGTTTCTGAA ATCAAGCAAA GGTGGGTTCT TTGGAGATGG 368 TATCAAGTGG AACTTCTCCA AATTCCTCAT TGACAAAGAA GGACATGTCG TTGATCGCTA 23838 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATCAAGTGG AACTTCTCCA AATTCCTCAT TGACAAAGAA GGACATGTCG TTGATCGCTA 428 CTCTCCAACC ACTTCTCCAG CTAGCATGGA GGTAAGAATC CAGTGTCCTG TCCTATGATA 23898 |||||||||| |||| ||||| |||||||||| | CTCTCCAACC ACTTGTCCAG CTAGCATGGA G......... .......... .......... 459 AGCTTCTTCA CTCAAAAAAT TGTTCACTGA CGATATAGCT AATAATACTC GAACTCTTTG 23958 .......... .......... .......... .......... .......... .......... 459 TATTCCTGCA GAAGGATATC AAGAAACTCT TGGGTGTTGC TTAAGCTCTA GAGTCTGGAC 24018 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .AAGGATATC AAGAAACTCT TGGGTGTTGC TTAAGCTCTA GAGTCTGGAC 508 TCGGCGGTGC TGTCTCTGCA TTTTGTGCTA GAAATAATGA ACATATCATT GCGAATAAGC 24078 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGGCGGTGC TGTCTCTGCA TTTTGTGCTA GAAATAATGA ACATATCATT GCGAATAAGC 568 TATGTTTGAT GTA 24091 |||||||||| ||| TATGTTTGAT GTA 581 hqPGS_C08HBa0251H04.1+_SGN-E276156+ (21499 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24091) ******************************************************************************** EST sequence 120 -strand 574 n (File: SGN-E290365-) 1 TCATTGTCAA TGTTGCATCA CAGTGTGGCC TGACTAATTC GAACTACACT GACATGACGG 61 AGTTATACAA GAAGTACAAG GATCAAGGTT TGGAGATTCT GGCCTTCCCT TGCAACCAGT 121 TTGGTGGACA GGAGCCTGGA AACATTGAAG ATATCCAGCA AATGGTCTGC ACTCGCTTCA 181 AGGCTGAGTA CCCCATATTT GACAAGGTTG ACGTGAATGG TGATAATGCT GCTCCATTAT 241 ATAGGTTTCT GAAATCAAGC AAAGGTGGGT TCTTTGGAGA TGGTATCAAG TGGAACTTCT 301 CCAAATTCCT CATTGACAAA GAAGGACATG TCGTTGATCG CTACTCTCCA ACCACTTCTC 361 CAGCTAGCAT GGAGAAGGAT ATCAAGAAAC TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG 421 GACTCGGCGG TGCTGTCTCT GCATTTTGTG CTAGAAATAA TGAACATATC ATTGCGAATA 481 AGCTATGTTT GATGTAATTT TCTTCTTGTT TTAGTTCCTA CAGATGTTTG CTACTTTGTA 541 CAATTTCAAT ATACTGATCA ATATTTCTAT TTAA Predicted gene structure (within gDNA segment 22407 to 24778): Exon 1 22552 22576 ( 25 n); cDNA 1 25 ( 25 n); score: 1.000 Intron 1 22577 23257 ( 681 n); Pd: 0.958 (s: 0), Pa: 1.000 (s: 1.00) Exon 2 23258 23319 ( 62 n); cDNA 26 87 ( 62 n); score: 1.000 Intron 2 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23469 23587 ( 119 n); cDNA 88 206 ( 119 n); score: 1.000 Intron 3 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 23702 23869 ( 168 n); cDNA 207 374 ( 168 n); score: 1.000 Intron 4 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 5 23970 24168 ( 199 n); cDNA 375 573 ( 199 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E290365- 1.000 573 0.998 C PGS_C08HBa0251H04.1+_SGN-E290365- (22552 22576,23258 23319,23469 23587,23702 23869,23970 24168) Alignment (genomic DNA sequence = upper lines): TCATTGTCAA TGTTGCATCA CAGTGGTATG TATTAGTTTC GTAGTTATTT TATTCATTAT 22611 |||||||||| |||||||||| ||||| TCATTGTCAA TGTTGCATCA CAGTG..... .......... .......... .......... 25 ATGTTTGAAG CCTCTTCACA ATATTTTGGA TTTCAATAGG AACTTTCGAT GAGAGAAATG 22671 .......... .......... .......... .......... .......... .......... 25 ATCTGATCGC TAGTTTTTGC TCCTTGTAAT TAAGTGAAAT TTTCATGTGT TGCTCTCGAT 22731 .......... .......... .......... .......... .......... .......... 25 AGAATTGACT CAAAGTAAAG AAGTATTAAA CTTGAACCTT ATGCTACATC AGATAGGGCA 22791 .......... .......... .......... .......... .......... .......... 25 TTCCTCCCCG TGATGAGAAT ACTATGCATT GTGATAATAA CTGAATTTAC GAGATATTTA 22851 .......... .......... .......... .......... .......... .......... 25 GTGAAGTTTT CAGGTGTTGT CCTCCATAGA ATTGACTGGA AGTAAAAAAG TATTAAAATT 22911 .......... .......... .......... .......... .......... .......... 25 GAACCTTATC TTACACTGGA TAGGGCGTTC CTCCTTGTGA TGAAAATACT ATGCATTTTT 22971 .......... .......... .......... .......... .......... .......... 25 ATAAAAACTG AATCTACGAG AAATTGAACA TGACATTATT TTACTCGAGC CCTGACTAAA 23031 .......... .......... .......... .......... .......... .......... 25 CATTCTTACT ATGCTTGGTC CACTACTCAA CAAACGTGTT CTTAGTACCA CTAATGACAA 23091 .......... .......... .......... .......... .......... .......... 25 GTGATTGTGC ATATCCAGAT AAGATGTCTT TTTCATTTCG GTATAATTTG ATTTCATTGT 23151 .......... .......... .......... .......... .......... .......... 25 GTTTACTGTG AAAGGTTTGA GCAGTTACTT GTTGTATTTA TGATATCATT CTTTGATCTT 23211 .......... .......... .......... .......... .......... .......... 25 GTTACATTTG CTTATGACTG TTAACTGATT CCATTTTCTG ATGCAGTGGC CTGACTAATT 23271 |||| |||||||||| .......... .......... .......... .......... ......TGGC CTGACTAATT 39 CGAACTACAC TGACATGACG GAGTTATACA AGAAGTACAA GGATCAAGGT TTGTCCAATT 23331 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| CGAACTACAC TGACATGACG GAGTTATACA AGAAGTACAA GGATCAAG.. .......... 87 CTCCCGAACC AACTGTGCCC TCGATTCTCC AGCAATGGAG GCGTTCGCCC TGACATATTT 23391 .......... .......... .......... .......... .......... .......... 87 CTGCAAAGAG AACTTAAATA AAATGGATGT TTATATCTAA TTTAGCTTGT TAATTATATG 23451 .......... .......... .......... .......... .......... .......... 87 TTCTTCCTGT TACACAGGTT TGGAGATTCT GGCCTTCCCT TGCAACCAGT TTGGTGGACA 23511 ||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .......GTT TGGAGATTCT GGCCTTCCCT TGCAACCAGT TTGGTGGACA 130 GGAGCCTGGA AACATTGAAG ATATCCAGCA AATGGTCTGC ACTCGCTTCA AGGCTGAGTA 23571 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAGCCTGGA AACATTGAAG ATATCCAGCA AATGGTCTGC ACTCGCTTCA AGGCTGAGTA 190 CCCCATATTT GACAAGGTAG AAATAACACG AAGCATTCTT CTCTTCTAGT CATCTACACT 23631 |||||||||| |||||| CCCCATATTT GACAAG.... .......... .......... .......... .......... 206 ACAGCTGAAA CTGAGAATAA ATGAGGCAAG CTGTATGTAC TGACATCTTC GTGTGTTTTG 23691 .......... .......... .......... .......... .......... .......... 206 TGTGGTCTAG GTTGACGTGA ATGGTGATAA TGCTGCTCCA TTATATAGGT TTCTGAAATC 23751 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... GTTGACGTGA ATGGTGATAA TGCTGCTCCA TTATATAGGT TTCTGAAATC 256 AAGCAAAGGT GGGTTCTTTG GAGATGGTAT CAAGTGGAAC TTCTCCAAAT TCCTCATTGA 23811 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCAAAGGT GGGTTCTTTG GAGATGGTAT CAAGTGGAAC TTCTCCAAAT TCCTCATTGA 316 CAAAGAAGGA CATGTCGTTG ATCGCTACTC TCCAACCACT TCTCCAGCTA GCATGGAGGT 23871 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| CAAAGAAGGA CATGTCGTTG ATCGCTACTC TCCAACCACT TCTCCAGCTA GCATGGAG.. 374 AAGAATCCAG TGTCCTGTCC TATGATAAGC TTCTTCACTC AAAAAATTGT TCACTGACGA 23931 .......... .......... .......... .......... .......... .......... 374 TATAGCTAAT AATACTCGAA CTCTTTGTAT TCCTGCAGAA GGATATCAAG AAACTCTTGG 23991 || |||||||||| |||||||||| .......... .......... .......... ........AA GGATATCAAG AAACTCTTGG 396 GTGTTGCTTA AGCTCTAGAG TCTGGACTCG GCGGTGCTGT CTCTGCATTT TGTGCTAGAA 24051 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGTTGCTTA AGCTCTAGAG TCTGGACTCG GCGGTGCTGT CTCTGCATTT TGTGCTAGAA 456 ATAATGAACA TATCATTGCG AATAAGCTAT GTTTGATGTA ATTTTCTTCT TGTTTTAGTT 24111 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAATGAACA TATCATTGCG AATAAGCTAT GTTTGATGTA ATTTTCTTCT TGTTTTAGTT 516 CCTACAGATG TTTGCTACTT TGTACAATTT CAATATACTG ATCAATATTT CTATTTA 24168 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| CCTACAGATG TTTGCTACTT TGTACAATTT CAATATACTG ATCAATATTT CTATTTA 573 hqPGS_C08HBa0251H04.1+_SGN-E290365- (22552 22576,23258 23319,23469 23587,23702 23869,23970 24168) ******************************************************************************** EST sequence 57 +strand 602 n (File: SGN-E284082+) 1 CATTGTCAAT GTTGCATCAC AGTGTGGCCT GACTAATTCG AACTACACTG ACATGACGGA 61 GTTATACAAG AAGTACAAGG ATCAAGGTTT GGAGATTCTG GCCTTCCCTT GCAACCAGTT 121 TGGTGGACAG GAGCCTGGAA ACATTGAAGA TATCCAGCAA ATGGTCTGCA CTCGCTTCAA 181 GGCTGAGTAC CCCATATTTG ACAAGGTTGA CGTGAATGGT GATAATGCTG CTCCATTATA 241 TAGGTTTCTG AAATCAAGCA AAGGTGGGTT CTTTGGAGAT GGTATCAAGT GGAACTTCTC 301 CAAATTCCTC ATTGACAAAG AAGGACATGT CGTTGATCGC TACTCTCCAA CCACTTCTCC 361 AGCTAGCATG GAGAAGGATA TCAAGAAACT CTTGGGTGTT GCTTAAGCTC TAGAGTCTGG 421 ACTCGGCGGT GCTGTCTCTG CATTTTGTGC TAGAAATAAT GAACATATCA TTGCGAATAA 481 GCTATGTTTG ATGTAATTTT CTTCTTGTTT TAGTTCCTAC AGATGTTTGC TACTTTGTAC 541 AATTTCAATA TACTGATCAA TATTTCTATT TACAAGTATT ATCAAGTTTT TTCCAACTTT 601 TG Predicted gene structure (within gDNA segment 22427 to 24808): Exon 1 22553 22576 ( 24 n); cDNA 1 24 ( 24 n); score: 1.000 Intron 1 22577 23257 ( 681 n); Pd: 0.958 (s: 0), Pa: 1.000 (s: 1.00) Exon 2 23258 23319 ( 62 n); cDNA 25 86 ( 62 n); score: 1.000 Intron 2 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 3 23469 23587 ( 119 n); cDNA 87 205 ( 119 n); score: 1.000 Intron 3 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 23702 23869 ( 168 n); cDNA 206 373 ( 168 n); score: 1.000 Intron 4 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 5 23970 24198 ( 229 n); cDNA 374 602 ( 229 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E284082+ 1.000 602 1.000 C PGS_C08HBa0251H04.1+_SGN-E284082+ (22553 22576,23258 23319,23469 23587,23702 23869,23970 24198) Alignment (genomic DNA sequence = upper lines): CATTGTCAAT GTTGCATCAC AGTGGTATGT ATTAGTTTCG TAGTTATTTT ATTCATTATA 22612 |||||||||| |||||||||| |||| CATTGTCAAT GTTGCATCAC AGTG...... .......... .......... .......... 24 TGTTTGAAGC CTCTTCACAA TATTTTGGAT TTCAATAGGA ACTTTCGATG AGAGAAATGA 22672 .......... .......... .......... .......... .......... .......... 24 TCTGATCGCT AGTTTTTGCT CCTTGTAATT AAGTGAAATT TTCATGTGTT GCTCTCGATA 22732 .......... .......... .......... .......... .......... .......... 24 GAATTGACTC AAAGTAAAGA AGTATTAAAC TTGAACCTTA TGCTACATCA GATAGGGCAT 22792 .......... .......... .......... .......... .......... .......... 24 TCCTCCCCGT GATGAGAATA CTATGCATTG TGATAATAAC TGAATTTACG AGATATTTAG 22852 .......... .......... .......... .......... .......... .......... 24 TGAAGTTTTC AGGTGTTGTC CTCCATAGAA TTGACTGGAA GTAAAAAAGT ATTAAAATTG 22912 .......... .......... .......... .......... .......... .......... 24 AACCTTATCT TACACTGGAT AGGGCGTTCC TCCTTGTGAT GAAAATACTA TGCATTTTTA 22972 .......... .......... .......... .......... .......... .......... 24 TAAAAACTGA ATCTACGAGA AATTGAACAT GACATTATTT TACTCGAGCC CTGACTAAAC 23032 .......... .......... .......... .......... .......... .......... 24 ATTCTTACTA TGCTTGGTCC ACTACTCAAC AAACGTGTTC TTAGTACCAC TAATGACAAG 23092 .......... .......... .......... .......... .......... .......... 24 TGATTGTGCA TATCCAGATA AGATGTCTTT TTCATTTCGG TATAATTTGA TTTCATTGTG 23152 .......... .......... .......... .......... .......... .......... 24 TTTACTGTGA AAGGTTTGAG CAGTTACTTG TTGTATTTAT GATATCATTC TTTGATCTTG 23212 .......... .......... .......... .......... .......... .......... 24 TTACATTTGC TTATGACTGT TAACTGATTC CATTTTCTGA TGCAGTGGCC TGACTAATTC 23272 ||||| |||||||||| .......... .......... .......... .......... .....TGGCC TGACTAATTC 39 GAACTACACT GACATGACGG AGTTATACAA GAAGTACAAG GATCAAGGTT TGTCCAATTC 23332 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| GAACTACACT GACATGACGG AGTTATACAA GAAGTACAAG GATCAAG... .......... 86 TCCCGAACCA ACTGTGCCCT CGATTCTCCA GCAATGGAGG CGTTCGCCCT GACATATTTC 23392 .......... .......... .......... .......... .......... .......... 86 TGCAAAGAGA ACTTAAATAA AATGGATGTT TATATCTAAT TTAGCTTGTT AATTATATGT 23452 .......... .......... .......... .......... .......... .......... 86 TCTTCCTGTT ACACAGGTTT GGAGATTCTG GCCTTCCCTT GCAACCAGTT TGGTGGACAG 23512 |||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ......GTTT GGAGATTCTG GCCTTCCCTT GCAACCAGTT TGGTGGACAG 130 GAGCCTGGAA ACATTGAAGA TATCCAGCAA ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC 23572 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGCCTGGAA ACATTGAAGA TATCCAGCAA ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC 190 CCCATATTTG ACAAGGTAGA AATAACACGA AGCATTCTTC TCTTCTAGTC ATCTACACTA 23632 |||||||||| ||||| CCCATATTTG ACAAG..... .......... .......... .......... .......... 205 CAGCTGAAAC TGAGAATAAA TGAGGCAAGC TGTATGTACT GACATCTTCG TGTGTTTTGT 23692 .......... .......... .......... .......... .......... .......... 205 GTGGTCTAGG TTGACGTGAA TGGTGATAAT GCTGCTCCAT TATATAGGTT TCTGAAATCA 23752 | |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .........G TTGACGTGAA TGGTGATAAT GCTGCTCCAT TATATAGGTT TCTGAAATCA 256 AGCAAAGGTG GGTTCTTTGG AGATGGTATC AAGTGGAACT TCTCCAAATT CCTCATTGAC 23812 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCAAAGGTG GGTTCTTTGG AGATGGTATC AAGTGGAACT TCTCCAAATT CCTCATTGAC 316 AAAGAAGGAC ATGTCGTTGA TCGCTACTCT CCAACCACTT CTCCAGCTAG CATGGAGGTA 23872 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| AAAGAAGGAC ATGTCGTTGA TCGCTACTCT CCAACCACTT CTCCAGCTAG CATGGAG... 373 AGAATCCAGT GTCCTGTCCT ATGATAAGCT TCTTCACTCA AAAAATTGTT CACTGACGAT 23932 .......... .......... .......... .......... .......... .......... 373 ATAGCTAATA ATACTCGAAC TCTTTGTATT CCTGCAGAAG GATATCAAGA AACTCTTGGG 23992 ||| |||||||||| |||||||||| .......... .......... .......... .......AAG GATATCAAGA AACTCTTGGG 396 TGTTGCTTAA GCTCTAGAGT CTGGACTCGG CGGTGCTGTC TCTGCATTTT GTGCTAGAAA 24052 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTGCTTAA GCTCTAGAGT CTGGACTCGG CGGTGCTGTC TCTGCATTTT GTGCTAGAAA 456 TAATGAACAT ATCATTGCGA ATAAGCTATG TTTGATGTAA TTTTCTTCTT GTTTTAGTTC 24112 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATGAACAT ATCATTGCGA ATAAGCTATG TTTGATGTAA TTTTCTTCTT GTTTTAGTTC 516 CTACAGATGT TTGCTACTTT GTACAATTTC AATATACTGA TCAATATTTC TATTTACAAG 24172 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTACAGATGT TTGCTACTTT GTACAATTTC AATATACTGA TCAATATTTC TATTTACAAG 576 TATTATCAAG TTTTTTCCAA CTTTTG 24198 |||||||||| |||||||||| |||||| TATTATCAAG TTTTTTCCAA CTTTTG 602 hqPGS_C08HBa0251H04.1+_SGN-E284082+ (22553 22576,23258 23319,23469 23587,23702 23869,23970 24198) ******************************************************************************** EST sequence 76 -strand 686 n (File: SGN-E371608-) 1 CGGTATAATT TGATTTCATT GTGTTTACTG TGAAAGGTTT GAGCAGTTAC TTGTTGTATT 61 TATGATATCA TTCTTTGATC TTGTTACATT TGCTTATGAC TGTTAACTGA TTCCATTTTC 121 TGATGCAGTG GCCTGACTAA TTCGAACTAC ACTGACATGA CGGAGTTATA CAAGAAGTAC 181 AAGGATCAAG GTTTGGAGAT TCTGGCCTTC CCTTGCAACC AGTTTGGTGG ACAGGAGCCT 241 GGAAACATTG AAGATATCCA GCAAATGGTC TGCACTCGCT TCAAGGCTGA GTACCCCATA 301 TTTGACAAGG TTGACGTGAA TGGTGATAAT GCTGCTCCAT TATATAGGTT TCTGAAATCA 361 AGCAAAGGTG GGTTCTTTGG AGATGGTATC AAGTGGAACT TCTCCAAATT CCTCATTGAC 421 AAAGAAGGAC ATGTCGTTGA TCGCTACTCT CCAACCACTT CTCCAGCTAG CATGGAGAAG 481 GATATCAAGA AACTCTTGGG TGTTGCTTAA GCTCTAGAGT CTGGACTCGG CGGTGCTGTC 541 TCTGCATTTT GTGCTAGAAA TAATGAACAT ATCATTGCGA ATAAGCTATG TTTGATGTAA 601 TTTTCTTCTT GTTTTAGTTC CTACAGATGT TTGCTACTTT GTACAATTTC AATATACTGA 661 TCAATATTTC TATTTACAAA AAAAAA Predicted gene structure (within gDNA segment 22520 to 24841): Exon 1 23130 23319 ( 190 n); cDNA 1 190 ( 190 n); score: 1.000 Intron 1 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 2 23469 23587 ( 119 n); cDNA 191 309 ( 119 n); score: 1.000 Intron 2 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 3 23702 23869 ( 168 n); cDNA 310 477 ( 168 n); score: 1.000 Intron 3 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 4 23970 24171 ( 202 n); cDNA 478 679 ( 202 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E371608- 1.000 679 0.990 C PGS_C08HBa0251H04.1+_SGN-E371608- (23130 23319,23469 23587,23702 23869,23970 24171) Alignment (genomic DNA sequence = upper lines): CGGTATAATT TGATTTCATT GTGTTTACTG TGAAAGGTTT GAGCAGTTAC TTGTTGTATT 23189 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGGTATAATT TGATTTCATT GTGTTTACTG TGAAAGGTTT GAGCAGTTAC TTGTTGTATT 60 TATGATATCA TTCTTTGATC TTGTTACATT TGCTTATGAC TGTTAACTGA TTCCATTTTC 23249 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATGATATCA TTCTTTGATC TTGTTACATT TGCTTATGAC TGTTAACTGA TTCCATTTTC 120 TGATGCAGTG GCCTGACTAA TTCGAACTAC ACTGACATGA CGGAGTTATA CAAGAAGTAC 23309 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATGCAGTG GCCTGACTAA TTCGAACTAC ACTGACATGA CGGAGTTATA CAAGAAGTAC 180 AAGGATCAAG GTTTGTCCAA TTCTCCCGAA CCAACTGTGC CCTCGATTCT CCAGCAATGG 23369 |||||||||| AAGGATCAAG .......... .......... .......... .......... .......... 190 AGGCGTTCGC CCTGACATAT TTCTGCAAAG AGAACTTAAA TAAAATGGAT GTTTATATCT 23429 .......... .......... .......... .......... .......... .......... 190 AATTTAGCTT GTTAATTATA TGTTCTTCCT GTTACACAGG TTTGGAGATT CTGGCCTTCC 23489 | |||||||||| |||||||||| .......... .......... .......... .........G TTTGGAGATT CTGGCCTTCC 211 CTTGCAACCA GTTTGGTGGA CAGGAGCCTG GAAACATTGA AGATATCCAG CAAATGGTCT 23549 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGCAACCA GTTTGGTGGA CAGGAGCCTG GAAACATTGA AGATATCCAG CAAATGGTCT 271 GCACTCGCTT CAAGGCTGAG TACCCCATAT TTGACAAGGT AGAAATAACA CGAAGCATTC 23609 |||||||||| |||||||||| |||||||||| |||||||| GCACTCGCTT CAAGGCTGAG TACCCCATAT TTGACAAG.. .......... .......... 309 TTCTCTTCTA GTCATCTACA CTACAGCTGA AACTGAGAAT AAATGAGGCA AGCTGTATGT 23669 .......... .......... .......... .......... .......... .......... 309 ACTGACATCT TCGTGTGTTT TGTGTGGTCT AGGTTGACGT GAATGGTGAT AATGCTGCTC 23729 |||||||| |||||||||| |||||||||| .......... .......... .......... ..GTTGACGT GAATGGTGAT AATGCTGCTC 337 CATTATATAG GTTTCTGAAA TCAAGCAAAG GTGGGTTCTT TGGAGATGGT ATCAAGTGGA 23789 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTATATAG GTTTCTGAAA TCAAGCAAAG GTGGGTTCTT TGGAGATGGT ATCAAGTGGA 397 ACTTCTCCAA ATTCCTCATT GACAAAGAAG GACATGTCGT TGATCGCTAC TCTCCAACCA 23849 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTTCTCCAA ATTCCTCATT GACAAAGAAG GACATGTCGT TGATCGCTAC TCTCCAACCA 457 CTTCTCCAGC TAGCATGGAG GTAAGAATCC AGTGTCCTGT CCTATGATAA GCTTCTTCAC 23909 |||||||||| |||||||||| CTTCTCCAGC TAGCATGGAG .......... .......... .......... .......... 477 TCAAAAAATT GTTCACTGAC GATATAGCTA ATAATACTCG AACTCTTTGT ATTCCTGCAG 23969 .......... .......... .......... .......... .......... .......... 477 AAGGATATCA AGAAACTCTT GGGTGTTGCT TAAGCTCTAG AGTCTGGACT CGGCGGTGCT 24029 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGATATCA AGAAACTCTT GGGTGTTGCT TAAGCTCTAG AGTCTGGACT CGGCGGTGCT 537 GTCTCTGCAT TTTGTGCTAG AAATAATGAA CATATCATTG CGAATAAGCT ATGTTTGATG 24089 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTCTGCAT TTTGTGCTAG AAATAATGAA CATATCATTG CGAATAAGCT ATGTTTGATG 597 TAATTTTCTT CTTGTTTTAG TTCCTACAGA TGTTTGCTAC TTTGTACAAT TTCAATATAC 24149 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATTTTCTT CTTGTTTTAG TTCCTACAGA TGTTTGCTAC TTTGTACAAT TTCAATATAC 657 TGATCAATAT TTCTATTTAC AA 24171 |||||||||| |||||||||| || TGATCAATAT TTCTATTTAC AA 679 hqPGS_C08HBa0251H04.1+_SGN-E371608- (23130 23319,23469 23587,23702 23869,23970 24171) ******************************************************************************** EST sequence 83 -strand 599 n (File: SGN-E280324-) 1 ATTTGCTTAT GACTGTTAAC TGATTCCATT TTCTGATGCA GTGGCCTGAC TAATTCGAAC 61 TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAGGTTTGGA GATTCTGGCC 121 TTCCCTTGCA ACCAGTTTGG TGGACAGGAG CCTGGAAACA TTGAAGATAT CCAGCAAATG 181 GTCTGCACTC GCTTCAAGGC TGAGTACCCC ATATTTGACA AGGTTGACGT GAATGGTGAT 241 AATGCTGCTC CATTATATAG GTTTCTGAAA TCAAGCAAAG GTGGGTTCTT TGGAGATGGT 301 ATCAAGTGGA ACTTCTCCAA ATTCCTCATT GACAAAGAAG GACATGTCGT TGATCGCTAC 361 TCTCCAACCA CTTCTCCAGC TAGCATGGAG AAGGATATCA AGAAACTCTT GGGTGTTGCT 421 TAAGCTCTAG AGTCTGGACT CGGCGGTGCT GTCTCTGCAT TTTGTGCTAG AAATAATGAA 481 CATATCATTG CGAATAAGCT ATGTTTGATG TAATTTTCTT CTTGTTTTAG TTCCTACAGA 541 TGTTTGCTAC TTTGTACAAT TTCAATATAC TGATCAATAT TTCTATTTAC AAAAAAAAA Predicted gene structure (within gDNA segment 22607 to 24841): Exon 1 23217 23319 ( 103 n); cDNA 1 103 ( 103 n); score: 1.000 Intron 1 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 2 23469 23587 ( 119 n); cDNA 104 222 ( 119 n); score: 1.000 Intron 2 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 3 23702 23869 ( 168 n); cDNA 223 390 ( 168 n); score: 1.000 Intron 3 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 4 23970 24171 ( 202 n); cDNA 391 592 ( 202 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E280324- 1.000 592 0.988 C PGS_C08HBa0251H04.1+_SGN-E280324- (23217 23319,23469 23587,23702 23869,23970 24171) Alignment (genomic DNA sequence = upper lines): ATTTGCTTAT GACTGTTAAC TGATTCCATT TTCTGATGCA GTGGCCTGAC TAATTCGAAC 23276 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTGCTTAT GACTGTTAAC TGATTCCATT TTCTGATGCA GTGGCCTGAC TAATTCGAAC 60 TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAGGTTTGTC CAATTCTCCC 23336 |||||||||| |||||||||| |||||||||| |||||||||| ||| TACACTGACA TGACGGAGTT ATACAAGAAG TACAAGGATC AAG....... .......... 103 GAACCAACTG TGCCCTCGAT TCTCCAGCAA TGGAGGCGTT CGCCCTGACA TATTTCTGCA 23396 .......... .......... .......... .......... .......... .......... 103 AAGAGAACTT AAATAAAATG GATGTTTATA TCTAATTTAG CTTGTTAATT ATATGTTCTT 23456 .......... .......... .......... .......... .......... .......... 103 CCTGTTACAC AGGTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC 23516 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..GTTTGGAG ATTCTGGCCT TCCCTTGCAA CCAGTTTGGT GGACAGGAGC 151 CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA 23576 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGGAAACAT TGAAGATATC CAGCAAATGG TCTGCACTCG CTTCAAGGCT GAGTACCCCA 211 TATTTGACAA GGTAGAAATA ACACGAAGCA TTCTTCTCTT CTAGTCATCT ACACTACAGC 23636 |||||||||| | TATTTGACAA G......... .......... .......... .......... .......... 222 TGAAACTGAG AATAAATGAG GCAAGCTGTA TGTACTGACA TCTTCGTGTG TTTTGTGTGG 23696 .......... .......... .......... .......... .......... .......... 222 TCTAGGTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA 23756 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....GTTGA CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA 277 AAGGTGGGTT CTTTGGAGAT GGTATCAAGT GGAACTTCTC CAAATTCCTC ATTGACAAAG 23816 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGTGGGTT CTTTGGAGAT GGTATCAAGT GGAACTTCTC CAAATTCCTC ATTGACAAAG 337 AAGGACATGT CGTTGATCGC TACTCTCCAA CCACTTCTCC AGCTAGCATG GAGGTAAGAA 23876 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| AAGGACATGT CGTTGATCGC TACTCTCCAA CCACTTCTCC AGCTAGCATG GAG....... 390 TCCAGTGTCC TGTCCTATGA TAAGCTTCTT CACTCAAAAA ATTGTTCACT GACGATATAG 23936 .......... .......... .......... .......... .......... .......... 390 CTAATAATAC TCGAACTCTT TGTATTCCTG CAGAAGGATA TCAAGAAACT CTTGGGTGTT 23996 ||||||| |||||||||| |||||||||| .......... .......... .......... ...AAGGATA TCAAGAAACT CTTGGGTGTT 417 GCTTAAGCTC TAGAGTCTGG ACTCGGCGGT GCTGTCTCTG CATTTTGTGC TAGAAATAAT 24056 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTAAGCTC TAGAGTCTGG ACTCGGCGGT GCTGTCTCTG CATTTTGTGC TAGAAATAAT 477 GAACATATCA TTGCGAATAA GCTATGTTTG ATGTAATTTT CTTCTTGTTT TAGTTCCTAC 24116 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAACATATCA TTGCGAATAA GCTATGTTTG ATGTAATTTT CTTCTTGTTT TAGTTCCTAC 537 AGATGTTTGC TACTTTGTAC AATTTCAATA TACTGATCAA TATTTCTATT TACAA 24171 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| AGATGTTTGC TACTTTGTAC AATTTCAATA TACTGATCAA TATTTCTATT TACAA 592 hqPGS_C08HBa0251H04.1+_SGN-E280324- (23217 23319,23469 23587,23702 23869,23970 24171) ******************************************************************************** EST sequence 85 -strand 467 n (File: SGN-E389614-) 1 GACTAATTCG AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT 61 GGAGATTCTG GCCTTCCCTT GCAACCAGTT TGGTGGACAG GAGCCTGGAA ACATTGAAGA 121 TATCCAGCAA ATGGTCTGCA CTCGCTTCAA GGCTGAGTAC CCCATATTTG ACAAGGTTGA 181 CGTGAATGGT GATAATGCTG CTCCATTATA TAGGTTTCTG AAATCAAGCA AAGGTGGGTT 241 CTTTGGAGAT GGTATCAAGT GGAACTTCTC CAAATTCCTC ATTGACAAAG AAGGACATGT 301 CGTTGATCGC TACTCTCCAA CCACTTCTCC AGCTAGCATG GAGAAGGATA TCAAGAAACT 361 CTTGGGTGTT GCTTAAGCTC TAGAGTCTGG ACTCGGCGGT GCTGTCTCTG CATTTTGTGC 421 TAGAAATAAT GAACATATCA TTGCGAATAA GCTATGTTTG ATGTAAT Predicted gene structure (within gDNA segment 22654 to 24693): Exon 1 23264 23319 ( 56 n); cDNA 1 56 ( 56 n); score: 1.000 Intron 1 23320 23468 ( 149 n); Pd: 0.000 (s: 1.00), Pa: 1.000 (s: 1.00) Exon 2 23469 23587 ( 119 n); cDNA 57 175 ( 119 n); score: 1.000 Intron 2 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 3 23702 23869 ( 168 n); cDNA 176 343 ( 168 n); score: 1.000 Intron 3 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 4 23970 24093 ( 124 n); cDNA 344 467 ( 124 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E389614- 1.000 467 1.000 C PGS_C08HBa0251H04.1+_SGN-E389614- (23264 23319,23469 23587,23702 23869,23970 24093) Alignment (genomic DNA sequence = upper lines): GACTAATTCG AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAGGTTT 23323 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| GACTAATTCG AACTACACTG ACATGACGGA GTTATACAAG AAGTACAAGG ATCAAG.... 56 GTCCAATTCT CCCGAACCAA CTGTGCCCTC GATTCTCCAG CAATGGAGGC GTTCGCCCTG 23383 .......... .......... .......... .......... .......... .......... 56 ACATATTTCT GCAAAGAGAA CTTAAATAAA ATGGATGTTT ATATCTAATT TAGCTTGTTA 23443 .......... .......... .......... .......... .......... .......... 56 ATTATATGTT CTTCCTGTTA CACAGGTTTG GAGATTCTGG CCTTCCCTTG CAACCAGTTT 23503 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....GTTTG GAGATTCTGG CCTTCCCTTG CAACCAGTTT 91 GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA TGGTCTGCAC TCGCTTCAAG 23563 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTGGACAGG AGCCTGGAAA CATTGAAGAT ATCCAGCAAA TGGTCTGCAC TCGCTTCAAG 151 GCTGAGTACC CCATATTTGA CAAGGTAGAA ATAACACGAA GCATTCTTCT CTTCTAGTCA 23623 |||||||||| |||||||||| |||| GCTGAGTACC CCATATTTGA CAAG...... .......... .......... .......... 175 TCTACACTAC AGCTGAAACT GAGAATAAAT GAGGCAAGCT GTATGTACTG ACATCTTCGT 23683 .......... .......... .......... .......... .......... .......... 175 GTGTTTTGTG TGGTCTAGGT TGACGTGAAT GGTGATAATG CTGCTCCATT ATATAGGTTT 23743 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........GT TGACGTGAAT GGTGATAATG CTGCTCCATT ATATAGGTTT 217 CTGAAATCAA GCAAAGGTGG GTTCTTTGGA GATGGTATCA AGTGGAACTT CTCCAAATTC 23803 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGAAATCAA GCAAAGGTGG GTTCTTTGGA GATGGTATCA AGTGGAACTT CTCCAAATTC 277 CTCATTGACA AAGAAGGACA TGTCGTTGAT CGCTACTCTC CAACCACTTC TCCAGCTAGC 23863 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCATTGACA AAGAAGGACA TGTCGTTGAT CGCTACTCTC CAACCACTTC TCCAGCTAGC 337 ATGGAGGTAA GAATCCAGTG TCCTGTCCTA TGATAAGCTT CTTCACTCAA AAAATTGTTC 23923 |||||| ATGGAG.... .......... .......... .......... .......... .......... 343 ACTGACGATA TAGCTAATAA TACTCGAACT CTTTGTATTC CTGCAGAAGG ATATCAAGAA 23983 |||| |||||||||| .......... .......... .......... .......... ......AAGG ATATCAAGAA 357 ACTCTTGGGT GTTGCTTAAG CTCTAGAGTC TGGACTCGGC GGTGCTGTCT CTGCATTTTG 24043 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTCTTGGGT GTTGCTTAAG CTCTAGAGTC TGGACTCGGC GGTGCTGTCT CTGCATTTTG 417 TGCTAGAAAT AATGAACATA TCATTGCGAA TAAGCTATGT TTGATGTAAT 24093 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTAGAAAT AATGAACATA TCATTGCGAA TAAGCTATGT TTGATGTAAT 467 hqPGS_C08HBa0251H04.1+_SGN-E389614- (23264 23319,23469 23587,23702 23869,23970 24093) ******************************************************************************** EST sequence 110 -strand 551 n (File: SGN-E287358-) 1 AGGATCAAGG TTTGGAGATT TTGGCCTTCC CTTGCAACCA GTTTGGTGGA CAGGAGCCTG 61 GAAACATTGA AGATATCCAG CAAATGGTCT GCACTCGCTT CAAGGCTGAG TACCCCATAT 121 TTGCCAAGGT TCACGTGAAT GGTGATAATG CTGCTCCATT AAATAGGTTT CTGAAATCAA 181 CCAAAGGTGG GTTCTTTGGA GATGGTATCA AGTGGAACTT CTCCAAATTC CTCATTGACA 241 AAAAAGGACA TGTCGTTGAT CGCTACTCTC CAACCACTTT TCCAGCTAGC ATGGAGAAGG 301 ATATCAAGAA ACTCTTGGGT GTTGCTTAAG CTCTAAAGTC TGGACTCGGC GGTGCTGTCT 361 TTGCATTTTG TGCTAGAAAT AATGAACATA TCCTTGCGAA TAAGCTATGT TTGAGGGAAT 421 TTTCTTCTTG TTTTAGTTCC CACAAATGTT TGCTACTTTG TACAATTTCA AAATACTGAC 481 CAATATTTCT TTTTAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 541 AAAAAACTCG A Predicted gene structure (within gDNA segment 22661 to 25544): Exon 1 23311 23319 ( 9 n); cDNA 1 9 ( 9 n); score: 1.000 Intron 1 23320 23468 ( 149 n); Pd: 0.000 (s: 0), Pa: 1.000 (s: 0.98) Exon 2 23469 23587 ( 119 n); cDNA 10 128 ( 119 n); score: 0.983 Intron 2 23588 23701 ( 114 n); Pd: 0.952 (s: 0.98), Pa: 0.998 (s: 0.96) Exon 3 23702 23869 ( 168 n); cDNA 129 296 ( 168 n); score: 0.970 Intron 3 23870 23969 ( 100 n); Pd: 1.000 (s: 0.98), Pa: 0.984 (s: 0.98) Exon 4 23970 24171 ( 202 n); cDNA 297 498 ( 202 n); score: 0.946 PPA cDNA 501 547 MATCH C08HBa0251H04.1+ SGN-E287358- 0.963 498 0.904 C PGS_C08HBa0251H04.1+_SGN-E287358- (23311 23319,23469 23587,23702 23869,23970 24171) Alignment (genomic DNA sequence = upper lines): AGGATCAAGG TTTGTCCAAT TCTCCCGAAC CAACTGTGCC CTCGATTCTC CAGCAATGGA 23370 ||||||||| AGGATCAAG. .......... .......... .......... .......... .......... 9 GGCGTTCGCC CTGACATATT TCTGCAAAGA GAACTTAAAT AAAATGGATG TTTATATCTA 23430 .......... .......... .......... .......... .......... .......... 9 ATTTAGCTTG TTAATTATAT GTTCTTCCTG TTACACAGGT TTGGAGATTC TGGCCTTCCC 23490 || ||||||||| |||||||||| .......... .......... .......... ........GT TTGGAGATTT TGGCCTTCCC 31 TTGCAACCAG TTTGGTGGAC AGGAGCCTGG AAACATTGAA GATATCCAGC AAATGGTCTG 23550 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGCAACCAG TTTGGTGGAC AGGAGCCTGG AAACATTGAA GATATCCAGC AAATGGTCTG 91 CACTCGCTTC AAGGCTGAGT ACCCCATATT TGACAAGGTA GAAATAACAC GAAGCATTCT 23610 |||||||||| |||||||||| |||||||||| || |||| CACTCGCTTC AAGGCTGAGT ACCCCATATT TGCCAAG... .......... .......... 128 TCTCTTCTAG TCATCTACAC TACAGCTGAA ACTGAGAATA AATGAGGCAA GCTGTATGTA 23670 .......... .......... .......... .......... .......... .......... 128 CTGACATCTT CGTGTGTTTT GTGTGGTCTA GGTTGACGTG AATGGTGATA ATGCTGCTCC 23730 ||| ||||| |||||||||| |||||||||| .......... .......... .......... .GTTCACGTG AATGGTGATA ATGCTGCTCC 157 ATTATATAGG TTTCTGAAAT CAAGCAAAGG TGGGTTCTTT GGAGATGGTA TCAAGTGGAA 23790 |||| ||||| |||||||||| ||| |||||| |||||||||| |||||||||| |||||||||| ATTAAATAGG TTTCTGAAAT CAACCAAAGG TGGGTTCTTT GGAGATGGTA TCAAGTGGAA 217 CTTCTCCAAA TTCCTCATTG ACAAAGAAGG ACATGTCGTT GATCGCTACT CTCCAACCAC 23850 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| CTTCTCCAAA TTCCTCATTG ACAAAAAAGG ACATGTCGTT GATCGCTACT CTCCAACCAC 277 TTCTCCAGCT AGCATGGAGG TAAGAATCCA GTGTCCTGTC CTATGATAAG CTTCTTCACT 23910 || ||||||| ||||||||| TTTTCCAGCT AGCATGGAG. .......... .......... .......... .......... 296 CAAAAAATTG TTCACTGACG ATATAGCTAA TAATACTCGA ACTCTTTGTA TTCCTGCAGA 23970 | .......... .......... .......... .......... .......... .........A 297 AGGATATCAA GAAACTCTTG GGTGTTGCTT AAGCTCTAGA GTCTGGACTC GGCGGTGCTG 24030 |||||||||| |||||||||| |||||||||| |||||||| | |||||||||| |||||||||| AGGATATCAA GAAACTCTTG GGTGTTGCTT AAGCTCTAAA GTCTGGACTC GGCGGTGCTG 357 TCTCTGCATT TTGTGCTAGA AATAATGAAC ATATCATTGC GAATAAGCTA TGTTTGATGT 24090 ||| |||||| |||||||||| |||||||||| ||||| |||| |||||||||| ||||||| | TCTTTGCATT TTGTGCTAGA AATAATGAAC ATATCCTTGC GAATAAGCTA TGTTTGAGGG 417 AATTTTCTTC TTGTTTTAGT TCCTACAGAT GTTTGCTACT TTGTACAATT TCAATATACT 24150 |||||||||| |||||||||| ||| ||| || |||||||||| |||||||||| |||| ||||| AATTTTCTTC TTGTTTTAGT TCCCACAAAT GTTTGCTACT TTGTACAATT TCAAAATACT 477 GATCAATATT TCTATTTACA A 24171 || ||||||| ||| |||| | | GACCAATATT TCTTTTTAAA A 498 hqPGS_C08HBa0251H04.1+_SGN-E287358- (23469 23587,23702 23869,23970 24171) ******************************************************************************** EST sequence 138 -strand 496 n (File: SGN-E349657-) 1 CTTGCAACCA GTTTGGTGGA CAGGAGCCTG GAAACATTGA AGATATCCAG CAAATGGTCT 61 GCACTCGCTT CAAGGCTGAG TACCCCATAT TTGACAAGGT TGACGTGAAT GGTGATAATG 121 CTGCTCCATT ATATAGGTTT CTGAAATCAA GCAAAGGTGG GTTCTTTGGA GATGGTATCA 181 AGTGGAACTT CTCCAAATTC CTCATTGACA AAGAAGGACA TGTCGTTGAT CGCTACTCTC 241 CAACCACTTC TCCAGCTAGC ATGGAGAAGG ATATCAAGAA ACTCTTGGGT GTTGCTTAAG 301 CTCTAGAGTC TGGACTCGGC GGTGCTGTCT CTGCATTTTG TGCTAGAAAT AATGAACATA 361 TCATTGCGAA TAAGCTATGT TTGATGTAAT TTTCTTCTTG TTTTAGTTCC TACAGATGTT 421 TGCTACTTTG TACAATTTCA ATATACTGAT CAATATTTCT ATTTACAAGT ATTATCAAGT 481 TTTTTCCAAA AAAAAA Predicted gene structure (within gDNA segment 22880 to 24862): Exon 1 23490 23587 ( 98 n); cDNA 1 98 ( 98 n); score: 1.000 Intron 1 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 2 23702 23869 ( 168 n); cDNA 99 266 ( 168 n); score: 1.000 Intron 2 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 3 23970 24192 ( 223 n); cDNA 267 489 ( 223 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E349657- 1.000 489 0.986 C PGS_C08HBa0251H04.1+_SGN-E349657- (23490 23587,23702 23869,23970 24192) Alignment (genomic DNA sequence = upper lines): CTTGCAACCA GTTTGGTGGA CAGGAGCCTG GAAACATTGA AGATATCCAG CAAATGGTCT 23549 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGCAACCA GTTTGGTGGA CAGGAGCCTG GAAACATTGA AGATATCCAG CAAATGGTCT 60 GCACTCGCTT CAAGGCTGAG TACCCCATAT TTGACAAGGT AGAAATAACA CGAAGCATTC 23609 |||||||||| |||||||||| |||||||||| |||||||| GCACTCGCTT CAAGGCTGAG TACCCCATAT TTGACAAG.. .......... .......... 98 TTCTCTTCTA GTCATCTACA CTACAGCTGA AACTGAGAAT AAATGAGGCA AGCTGTATGT 23669 .......... .......... .......... .......... .......... .......... 98 ACTGACATCT TCGTGTGTTT TGTGTGGTCT AGGTTGACGT GAATGGTGAT AATGCTGCTC 23729 |||||||| |||||||||| |||||||||| .......... .......... .......... ..GTTGACGT GAATGGTGAT AATGCTGCTC 126 CATTATATAG GTTTCTGAAA TCAAGCAAAG GTGGGTTCTT TGGAGATGGT ATCAAGTGGA 23789 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTATATAG GTTTCTGAAA TCAAGCAAAG GTGGGTTCTT TGGAGATGGT ATCAAGTGGA 186 ACTTCTCCAA ATTCCTCATT GACAAAGAAG GACATGTCGT TGATCGCTAC TCTCCAACCA 23849 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTTCTCCAA ATTCCTCATT GACAAAGAAG GACATGTCGT TGATCGCTAC TCTCCAACCA 246 CTTCTCCAGC TAGCATGGAG GTAAGAATCC AGTGTCCTGT CCTATGATAA GCTTCTTCAC 23909 |||||||||| |||||||||| CTTCTCCAGC TAGCATGGAG .......... .......... .......... .......... 266 TCAAAAAATT GTTCACTGAC GATATAGCTA ATAATACTCG AACTCTTTGT ATTCCTGCAG 23969 .......... .......... .......... .......... .......... .......... 266 AAGGATATCA AGAAACTCTT GGGTGTTGCT TAAGCTCTAG AGTCTGGACT CGGCGGTGCT 24029 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGATATCA AGAAACTCTT GGGTGTTGCT TAAGCTCTAG AGTCTGGACT CGGCGGTGCT 326 GTCTCTGCAT TTTGTGCTAG AAATAATGAA CATATCATTG CGAATAAGCT ATGTTTGATG 24089 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCTCTGCAT TTTGTGCTAG AAATAATGAA CATATCATTG CGAATAAGCT ATGTTTGATG 386 TAATTTTCTT CTTGTTTTAG TTCCTACAGA TGTTTGCTAC TTTGTACAAT TTCAATATAC 24149 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAATTTTCTT CTTGTTTTAG TTCCTACAGA TGTTTGCTAC TTTGTACAAT TTCAATATAC 446 TGATCAATAT TTCTATTTAC AAGTATTATC AAGTTTTTTC CAA 24192 |||||||||| |||||||||| |||||||||| |||||||||| ||| TGATCAATAT TTCTATTTAC AAGTATTATC AAGTTTTTTC CAA 489 hqPGS_C08HBa0251H04.1+_SGN-E349657- (23490 23587,23702 23869,23970 24192) ******************************************************************************** EST sequence 17 +strand 445 n (File: SGN-E294534+) 1 ATATCCAGCA AATGGTCTGC ACTCGCTTCA AGGCTGAGTA CCCCATATTT GACAAGGTTG 61 ACGTGAATGG TGATAATGCT GCTCCATTAT ATAGGTTTCT GAAATCAAGC AAAGGTGGGT 121 TCTTTGGAGA TGGTATCAAG TGGAACTTCT CCAAATTCCT CATTGACAAA GAAAGACATG 181 TCGTTGATCG CTACTCTCCA ACCACTTCTC CAGCTAGCAT GGAGAAGGAT ATCAAGAAAC 241 TCTTGGGTGT TGCTTAAGCT CTAGAGTCTG GACTCGGCGG TGCTGTCTCT GCATTTTGTG 301 CTAGAAATAA TGAACATATC ATTGCGAATA AGCTATGTTT GATGTAATTT TCTTCTTGTT 361 TTAGTTCCTA CAGATGTTTG CTACTTTGTA CAATTTCAAT ATACTGATCA ATATTTCTAT 421 TTNAAAAAAA AAAAAAAAAA AAAAA Predicted gene structure (within gDNA segment 22932 to 25007): Exon 1 23532 23587 ( 56 n); cDNA 1 56 ( 56 n); score: 1.000 Intron 1 23588 23701 ( 114 n); Pd: 0.952 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 2 23702 23869 ( 168 n); cDNA 57 224 ( 168 n); score: 0.994 Intron 2 23870 23969 ( 100 n); Pd: 1.000 (s: 1.00), Pa: 0.984 (s: 1.00) Exon 3 23970 24171 ( 202 n); cDNA 225 426 ( 202 n); score: 0.990 PPA cDNA 427 445 MATCH C08HBa0251H04.1+ SGN-E294534+ 0.993 426 0.957 C PGS_C08HBa0251H04.1+_SGN-E294534+ (23532 23587,23702 23869,23970 24171) Alignment (genomic DNA sequence = upper lines): ATATCCAGCA AATGGTCTGC ACTCGCTTCA AGGCTGAGTA CCCCATATTT GACAAGGTAG 23591 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| ATATCCAGCA AATGGTCTGC ACTCGCTTCA AGGCTGAGTA CCCCATATTT GACAAG.... 56 AAATAACACG AAGCATTCTT CTCTTCTAGT CATCTACACT ACAGCTGAAA CTGAGAATAA 23651 .......... .......... .......... .......... .......... .......... 56 ATGAGGCAAG CTGTATGTAC TGACATCTTC GTGTGTTTTG TGTGGTCTAG GTTGACGTGA 23711 |||||||||| .......... .......... .......... .......... .......... GTTGACGTGA 66 ATGGTGATAA TGCTGCTCCA TTATATAGGT TTCTGAAATC AAGCAAAGGT GGGTTCTTTG 23771 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGGTGATAA TGCTGCTCCA TTATATAGGT TTCTGAAATC AAGCAAAGGT GGGTTCTTTG 126 GAGATGGTAT CAAGTGGAAC TTCTCCAAAT TCCTCATTGA CAAAGAAGGA CATGTCGTTG 23831 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || |||||||||| GAGATGGTAT CAAGTGGAAC TTCTCCAAAT TCCTCATTGA CAAAGAAAGA CATGTCGTTG 186 ATCGCTACTC TCCAACCACT TCTCCAGCTA GCATGGAGGT AAGAATCCAG TGTCCTGTCC 23891 |||||||||| |||||||||| |||||||||| |||||||| ATCGCTACTC TCCAACCACT TCTCCAGCTA GCATGGAG.. .......... .......... 224 TATGATAAGC TTCTTCACTC AAAAAATTGT TCACTGACGA TATAGCTAAT AATACTCGAA 23951 .......... .......... .......... .......... .......... .......... 224 CTCTTTGTAT TCCTGCAGAA GGATATCAAG AAACTCTTGG GTGTTGCTTA AGCTCTAGAG 24011 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........AA GGATATCAAG AAACTCTTGG GTGTTGCTTA AGCTCTAGAG 266 TCTGGACTCG GCGGTGCTGT CTCTGCATTT TGTGCTAGAA ATAATGAACA TATCATTGCG 24071 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGGACTCG GCGGTGCTGT CTCTGCATTT TGTGCTAGAA ATAATGAACA TATCATTGCG 326 AATAAGCTAT GTTTGATGTA ATTTTCTTCT TGTTTTAGTT CCTACAGATG TTTGCTACTT 24131 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATAAGCTAT GTTTGATGTA ATTTTCTTCT TGTTTTAGTT CCTACAGATG TTTGCTACTT 386 TGTACAATTT CAATATACTG ATCAATATTT CTATTTACAA 24171 |||||||||| |||||||||| |||||||||| |||||| || TGTACAATTT CAATATACTG ATCAATATTT CTATTTNAAA 426 hqPGS_C08HBa0251H04.1+_SGN-E294534+ (23532 23587,23702 23869,23970 24171) ******************************************************************************** EST sequence 105 +strand 467 n (File: SGN-E310017+) 1 TGTTTTCTTT TCACTTTTCC TGTTTCATAC AGTTTTTTGC TTACTTTTTC ACTTAAAATT 61 ACTATCTTGG TGGTGTCTGT GTACTATAAA TCCTTGGCAT TTGTCAAAGC AAAGGTTAAA 121 TGTGTGATCT TTATAACTTC TGTTGGTTCA AAATTCAATC TTTATGTATT TTCAAGATTT 181 CATTTCTTTC TTCTAGTTTT TAGCTTGTTA ATCAACTTAC TGAAAAACCC ATCTTAAACC 241 CTTCTGAGGA TTTTAAGAAA AGTTGAATCT TTTTCATGGT TTGATTAGAT TTCTGTTGTT 301 GGGTTTTGTG TTCCATTTTG AATTGGCTGA TTGAACTTCA AAAAAGTTAT CACCTTTAGT 361 ACAATAGTAT AATCAAGACT CAGTTTTTCT TCTACTTTTG AAGTTGGGTT TTATGTTATT 421 GTTTGCACTT TATGTTCCAT TTTGAATTGG TTGATTGAAC TACATAA Predicted gene structure (within gDNA segment 30921 to 29228): Exon 1 30301 29838 ( 464 n); cDNA 3 467 ( 465 n); score: 0.973 MATCH C08HBa0251H04.1- SGN-E310017+ 0.973 464 0.994 C PGS_C08HBa0251H04.1-_SGN-E310017+ (30301 29838) Alignment (genomic DNA sequence = upper lines): TTTTCTTTTC ACTTTTCCTG TTTCATACAG TTTTTTGCTT ACTTTTTCAC TTAAAATTAC 30242 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTCTTTTC ACTTTTCCTG TTTCATACAG TTTTTTGCTT ACTTTTTCAC TTAAAATTAC 62 TATCTTGGTG GTGTTTTTGT ACTATAAATC CTTGGCATTT GTCAAAGCAA AGGTTAAATG 30182 |||||||||| |||| | ||| |||||||||| |||||||||| |||||||||| |||||||||| TATCTTGGTG GTGTCTGTGT ACTATAAATC CTTGGCATTT GTCAAAGCAA AGGTTAAATG 122 TGTGATCTTT AGAACTTCTG TTGGTTCAAA ATTCAATCTT TATGTATTTT CAAGATTTCA 30122 |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGATCTTT ATAACTTCTG TTGGTTCAAA ATTCAATCTT TATGTATTTT CAAGATTTCA 182 TTT-TTTTTT CTAGTTTTTA GCTTGTTAAT GAACTTACTG AAAAACCCAT CTTAAACCCT 30063 ||| ||| || |||||||||| |||||||||| ||||||||| |||||||||| |||||||||| TTTCTTTCTT CTAGTTTTTA GCTTGTTAAT CAACTTACTG AAAAACCCAT CTTAAACCCT 242 TCTGAGGATT TTAAGAAAAG TTGAATCTTT TTCATGGTTT GATTAGATTT CTGTTGTTGG 30003 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGAGGATT TTAAGAAAAG TTGAATCTTT TTCATGGTTT GATTAGATTT CTGTTGTTGG 302 GTTTTGTGTT CCATTTTGAA TTGGTTGATT GAACTTCAAA AAAGTTATCA CCTTTAGTAC 29943 |||||||||| |||||||||| |||| ||||| |||||||||| |||||||||| |||||||||| GTTTTGTGTT CCATTTTGAA TTGGCTGATT GAACTTCAAA AAAGTTATCA CCTTTAGTAC 362 AATAGTAGAA TCAAGACTCA GTTTTTCTTC TACTTTTGTT GTTGGGTTTT ATGTTATTGT 29883 ||||||| || |||||||||| |||||||||| |||||||| |||||||||| |||||||||| AATAGTATAA TCAAGACTCA GTTTTTCTTC TACTTTTGAA GTTGGGTTTT ATGTTATTGT 422 TTGTACTTTA TGTTCCATTT TGAATTGGTT GATTGAACTA CATAA 29838 ||| |||||| |||||||||| |||||||||| |||||||||| ||||| TTGCACTTTA TGTTCCATTT TGAATTGGTT GATTGAACTA CATAA 467 hqPGS_C08HBa0251H04.1-_SGN-E310017+ (30301 29838) ******************************************************************************** EST sequence 64 +strand 299 n (File: SGN-E578101+) 1 GTAAATAAAA AACCTTGTCC CTGAAGCCTC TAAATCTCAA AATTTAGCAA GTGGAGTTAT 61 ACATGTGGTA TGACTCCCTT TAAAACTAGT TTAGAAAAAA AAAACTCGAG ACTAGTTCTC 121 CTTTCATTTA TCAGTTAAAT GTTTTAGTAT TGTAACTTTC GAATTTTTCG AAATGTTTTG 181 TTTAGACATT CGCTACTGTT TTATTTAATT TAAAGACTTG GAGTTATATT GTTGTGCTTT 241 TATAATTTTT GTCTAGATTT ATTTAGATAT TGCATGAGTT AGAATGTTAA ATGTTGATT Predicted gene structure (within gDNA segment 35543 to 32355): Exon 1 33143 32965 ( 179 n); cDNA 121 299 ( 179 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E578101+ 1.000 179 0.599 C PGS_C08HBa0251H04.1-_SGN-E578101+ (33143 32965) Alignment (genomic DNA sequence = upper lines): CTTTCATTTA TCAGTTAAAT GTTTTAGTAT TGTAACTTTC GAATTTTTCG AAATGTTTTG 33084 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTTCATTTA TCAGTTAAAT GTTTTAGTAT TGTAACTTTC GAATTTTTCG AAATGTTTTG 180 TTTAGACATT CGCTACTGTT TTATTTAATT TAAAGACTTG GAGTTATATT GTTGTGCTTT 33024 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAGACATT CGCTACTGTT TTATTTAATT TAAAGACTTG GAGTTATATT GTTGTGCTTT 240 TATAATTTTT GTCTAGATTT ATTTAGATAT TGCATGAGTT AGAATGTTAA ATGTTGATT 32965 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TATAATTTTT GTCTAGATTT ATTTAGATAT TGCATGAGTT AGAATGTTAA ATGTTGATT 299 hqPGS_C08HBa0251H04.1-_SGN-E578101+ (33143 32965) ******************************************************************************** EST sequence 139 -strand 635 n (File: SGN-E318680-) 1 TAGACATAGG CGCCCAGCTA TCATACTCTA CCCACAGCTC CCTCAATGTA GAGAAATATA 61 CAGATATAGT GCTCATACCT TGACTGATTG TAGCAATTTC TTTGTGTAAT TGATAGATTC 121 GACTCCCATC CACTTTATCA AATCTTTCAC ACAGTTCCTT CCATACCTCT GCTGCATTAG 181 TTGAGTAAAC AATTCCAGTA AGCAACTCTT TTGATACACA ATTCATAATC CATGATAGAA 241 CAATGGCATT GCACCGTTCC CACATATTCT TTAAGTTTTC TCCATACGCT TCTTTCTTGC 301 AAGATCCATC GATGAATCCT AACTTGTTCC TTCCTAGAAT AACTATTTTC ATAGCTCTGC 361 TCCATACTGA GAAGTTTTCT GATCCAGATA GCTGGATCGA AATCAACATA ACACCTGAGT 421 TATCAATGTT ATTCAAGAAC AATGGATGAT TATGACTCAA TTTTTCTGGG AGTTCATTGT 481 CAACGACGAC TGCCATTGTT GCTTCTTCAA TCTTCCCAAT CTTCCAGATC TAATTTGAAC 541 CAGCTTTGGT TGAATCTACA GATGACTCTG ATACCATGTG AATTAATGTA GATTGAGAGT 601 ATGAAGAAGA GGAAGTAGTA TATTAACAGT AATTT Predicted gene structure (within gDNA segment 37166 to 40981): Exon 1 38254 38739 ( 486 n); cDNA 2 487 ( 486 n); score: 0.938 MATCH C08HBa0251H04.1+ SGN-E318680- 0.938 486 0.765 C PGS_C08HBa0251H04.1+_SGN-E318680- (38254 38739) Alignment (genomic DNA sequence = upper lines): AGACATAGGA ACCAATCTAT CATACTCTAC CCATAGCTCC CTCAATCGAG AAAAATATAC 38313 ||||||||| || | |||| |||||||||| ||| |||||| |||||| || | |||||||| AGACATAGGC GCCCAGCTAT CATACTCTAC CCACAGCTCC CTCAATGTAG AGAAATATAC 61 AGATATAGTG CTCATACCTT GACTGATTGT AGCAATTTCT TTGTGTAATT GATAAATTCA 38373 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| |||| AGATATAGTG CTCATACCTT GACTGATTGT AGCAATTTCT TTGTGTAATT GATAGATTCG 121 ACTCCCATCC ACTTTATCAT ATCTTTCACA CAGTTCCTTC CACACTTCTG CTGCATTAGT 38433 |||||||||| ||||||||| |||||||||| |||||||||| || || |||| |||||||||| ACTCCCATCC ACTTTATCAA ATCTTTCACA CAGTTCCTTC CATACCTCTG CTGCATTAGT 181 TGAGTAAACA ATTCCACTAA GCAACTCTTT TGATACACAA TTCATAATCC ATGATAGAAC 38493 |||||||||| |||||| ||| |||||||||| |||||||||| |||||||||| |||||||||| TGAGTAAACA ATTCCAGTAA GCAACTCTTT TGATACACAA TTCATAATCC ATGATAGAAC 241 AATGGCATTA CATCGTTCCC ACAGATTCTT CAAGTTTTAT CCATATGCTT CATTCTTGCA 38553 ||||||||| || ||||||| ||| |||||| ||||||| | ||||| |||| | |||||||| AATGGCATTG CACCGTTCCC ACATATTCTT TAAGTTTTCT CCATACGCTT CTTTCTTGCA 301 AGATCCATCG ATGAATCCTA GCTTATTTCT TCCTAGAATA GCTATTTTCA TAGCTCTGCT 38613 |||||||||| |||||||||| ||| || || |||||||||| ||||||||| |||||||||| AGATCCATCG ATGAATCCTA ACTTGTTCCT TCCTAGAATA ACTATTTTCA TAGCTCTGCT 361 CCACACTGAG AAGTTTTCTA ATCCAGATAG CTGGATCGAA ATCAACATAA CACCTGAGTT 38673 ||| |||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCATACTGAG AAGTTTTCTG ATCCAGATAG CTGGATCGAA ATCAACATAA CACCTGAGTT 421 ATCGATGTTA TTCAAGAACA ATGGATGATT ATGACTCAAT TTTTCTGGAA GTTCATTATC 38733 ||| |||||| |||||||||| |||||||||| |||||||||| |||||||| | ||||||| || ATCAATGTTA TTCAAGAACA ATGGATGATT ATGACTCAAT TTTTCTGGGA GTTCATTGTC 481 AACGAC 38739 |||||| AACGAC 487 hqPGS_C08HBa0251H04.1+_SGN-E318680- (38254 38739) ******************************************************************************** EST sequence 70 +strand 716 n (File: SGN-E314730+) 1 TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG TTCGAATTGG CTGACTCGAA 61 TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 121 AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 181 TCGTCAGAGT GAGAAATTTA TCGATGATTT CGAAAGAGGT AGACAACAAC TCCCTCAGAT 241 ACCAGTTTCA GCAGAAGCTT CATCAATGGC AATGCCAACA ATGCCATTGG GATCGAGTAT 301 AAATACTCCT CTAATAATCA AAGATGAAGA AGAACATAAA GATGAAAAGA TAAATCTGAA 361 TCCGTTGACA TTACTGAGTC CACCACCAAA TCAACGTGTT CGGATTAATG ATTTTTCATC 421 TCCTCACATC AACGAGGAAT CGAAGACTAT AATCTCTCAG GTCAAATCAG TTTTGGTGTT 481 CCAGCTCACC TCTGGAAGCA CGAAAAACAT TGAAGCAATG ATTAAGTTCG CGAACAATGC 541 ATTCTTCAAC TTGGATTTCC TTGAAGCAGA TTATTCATCT TTCTACAACA ATGTTAGAGA 601 TTTCATCGCG TGTCATTATG ATTTGCTCAT CGCTAAGAAC AGAGAGAAAT GCAGTCATTT 661 CCTATAGAGC TGAAGACTAG ATATGAAAAC GCGATAACTT GTGCAAATGA TCTTAA Predicted gene structure (within gDNA segment 45632 to 43119): Exon 1 45032 44776 ( 257 n); cDNA 1 257 ( 257 n); score: 1.000 Intron 1 44775 44563 ( 213 n); Pd: 1.000 (s: 1.00), Pa: 0.000 (s: 0.49) Exon 2 44562 44518 ( 45 n); cDNA 258 299 ( 42 n); score: 0.489 Intron 2 44517 44437 ( 81 n); Pd: 0.878 (s: 0.49), Pa: 0.840 (s: 0) Exon 3 44436 44400 ( 37 n); cDNA 300 335 ( 36 n); score: 0.568 Intron 3 44399 44094 ( 306 n); Pd: 0.308 (s: 0), Pa: 0.000 (s: 0.98) Exon 4 44093 43729 ( 365 n); cDNA 336 716 ( 381 n); score: 0.871 MATCH C08HBa0251H04.1- SGN-E314730+ 0.924 704 0.983 C PGS_C08HBa0251H04.1-_SGN-E314730+ (45032 44776,44562 44518,44436 44400,44093 43729) Alignment (genomic DNA sequence = upper lines): TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG TTCGAATTGG CTGACTCGAA 44973 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG TTCGAATTGG CTGACTCGAA 60 TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 44913 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 120 AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 44853 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 180 TCGTCAGAGT GAGAAATTTA TCGATGATTT CGAAAGAGGT AGACAACAAC TCCCTCAGAT 44793 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGTCAGAGT GAGAAATTTA TCGATGATTT CGAAAGAGGT AGACAACAAC TCCCTCAGAT 240 ACCAGTTTCA GCAGAAGGTA AGCTTCAATT TTGCCTTTTT TCGTGTTTAT GAGTTTTAAA 44733 |||||||||| ||||||| ACCAGTTTCA GCAGAAG... .......... .......... .......... .......... 257 TTCTAAAATA AAAAACTCAA TTCACTTGAT TTTAAATAAA TTATTTTATT AAATTATTAA 44673 .......... .......... .......... .......... .......... .......... 257 AATTATTGAG TTTTGTCAGA CCTGTAACTG TAACTATGGC TCCAGCCCCT GTCCCTACTA 44613 .......... .......... .......... .......... .......... .......... 257 CCCACTTTTG CTTCACTGCT ATTAGTTTTT CTTTTATGTA AAAAGTACTG GAACTTTAAT 44553 | | ||| .......... .......... .......... .......... .......... CTTCATCAAT 267 CGAAATTTCA GAGACATAGA CATAAAAATT ATTAAGTATA TACCTCTGAA CTCAGTTTTT 44493 | ||| || | | || | ||| || | GGCAATGCCA -ACA-AT-GC CATTGGGATC GAGTA..... .......... .......... 299 TCTTGTAATT TTATATATCT TGTGAATTAT GTGACATATT TCATAACTCC ACGCAGTTGA 44433 | | .......... .......... .......... .......... .......... ......TAAA 303 GACGCCTGAA ATATATTTGA AAGTAAAAAA AATGTATAAA ATTACAAAAA GAAAATGTGT 44373 || ||| | ||| || | | || || || TACTCCTCTA ATA-ATCAAA GATGAAGAAG AAC....... .......... .......... 335 TCAAGGACCG TAATTTAGTT ACCTTTATCC CTATTTTTAT TACATTATGG GAGGAGAGGG 44313 .......... .......... .......... .......... .......... .......... 335 AAAGGGTAAA ATAAGGAGCG CACCAAGTGA ACTAGCCCTC GATTTCGAGG TTTAGTGTTG 44253 .......... .......... .......... .......... .......... .......... 335 GTTCGGGTTC AATTGAATTC ATAATGTTAA AGTTTTGATA TTATTGATTT TGAAACAAAG 44193 .......... .......... .......... .......... .......... .......... 335 TATTGAAAAA TACTAAATCT TTTTGATATT GTTGATTTTG AAGCTTCATC AATGGCAATG 44133 .......... .......... .......... .......... .......... .......... 335 CCAACAATGC CATTGGGATC GAGTATAAAT ACTCTAATAA TCAAAGATGA AAAGATAAAT 44073 | | |||||||| |||||||||| .......... .......... .......... .........A T-AAAGATGA AAAGATAAAT 355 CTGAATCCGT TGACATTACT GAGTCCACCA CCAAATCAAC GTGTTCGGAT TAATGATTTT 44013 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGAATCCGT TGACATTACT GAGTCCACCA CCAAATCAAC GTGTTCGGAT TAATGATTTT 415 TCATCTCCTC ACATCAACGA GGAATCGAAG ACTATAATCT CTCAGGTCAA ATCAGTTTTG 43953 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCATCTCCTC ACATCAACGA GGAATCGAAG ACTATAATCT CTCAGGTCAA ATCAGTTTTG 475 GTGTTCCAGC TCACCTCTGG AAGCACGAAA AACATTGAAG CAATGATTAA GTTCGCGAAC 43893 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGTTCCAGC TCACCTCTGG AAGCACGAAA AACATTGAAG CAATGATTAA GTTCGCGAAC 535 AATGCATTCT TC-A-----A ----CTT--- G--G---ATT CATCTTTCTA CAACAATGTT 43851 |||||||||| || | | ||| | | ||| |||||||||| |||||||||| AATGCATTCT TCAACTTGGA TTTCCTTGAA GCAGATTATT CATCTTTCTA CAACAATGTT 595 AGAGATTTCA TCGCGTGTCA TTATGATTTG CTCATCGCTA AGAGACAGAG AGAAATGCAG 43791 |||||||||| |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| AGAGATTTCA TCGCGTGTCA TTATGATTTG CTCATCGCTA AGA-ACAGAG AGAAATGCAG 654 TCATTTCCTA TAGAGCTGAA GACTAGATAT GAAAACGCGA TAACTTGTGC AAATGATCTT 43731 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCATTTCCTA TAGAGCTGAA GACTAGATAT GAAAACGCGA TAACTTGTGC AAATGATCTT 714 AA 43729 || AA 716 hqPGS_C08HBa0251H04.1-_SGN-E314730+ (45032 44776,44562 44518,44436 44400,44093 43729) ******************************************************************************** EST sequence 71 +strand 544 n (File: SGN-E314578+) 1 TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG GTCGAATTGG CTGACTCGAA 61 TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 121 AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 181 TCGTGAGAGT GAGAAATTTA TCGATGATTT CAAAAGAGGT AGACAACAAC TCCCTCAGAT 241 ACCAGTTTCA GCAGAAGCTT CATCAATGGC AATGCCAACA ATGCCATTGG GATCGAGTAT 301 AAATACTCCT CTAATAATCA AAGATGAAGA AGAACATAAA GATGAAAAGA TAAATCTGAA 361 TCCGGTGACA TTACTGAGTC CACCACCAAA TCAACGTGTT CGGATTAATG AGTTTGCATC 421 TGCTCACATC AACGAGGAAT CGAAGACTAT AATCTCTCAG GTCAGATCAG ATCTGGTGTT 481 CCAGCTCACC TCTGGAAGCA CGAATTACAT TGAAGCAATG ATTAAGTTCG CGAACAATGC 541 ATTC Predicted gene structure (within gDNA segment 45632 to 43274): Exon 1 45032 44776 ( 257 n); cDNA 1 257 ( 257 n); score: 0.988 Intron 1 44775 44563 ( 213 n); Pd: 1.000 (s: 0.98), Pa: 0.000 (s: 0.49) Exon 2 44562 44518 ( 45 n); cDNA 258 299 ( 42 n); score: 0.489 Intron 2 44517 44437 ( 81 n); Pd: 0.878 (s: 0.49), Pa: 0.840 (s: 0) Exon 3 44436 44400 ( 37 n); cDNA 300 335 ( 36 n); score: 0.568 Intron 3 44399 44094 ( 306 n); Pd: 0.308 (s: 0), Pa: 0.000 (s: 0.96) Exon 4 44093 43884 ( 210 n); cDNA 336 544 ( 209 n); score: 0.952 MATCH C08HBa0251H04.1- SGN-E314578+ 0.972 549 1.009 C PGS_C08HBa0251H04.1-_SGN-E314578+ (45032 44776,44562 44518,44436 44400,44093 43884) Alignment (genomic DNA sequence = upper lines): TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG TTCGAATTGG CTGACTCGAA 44973 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| |||||||||| TTTTTTCCCC ATCTTTGAAC TCCAAAAATT ATAATTTACG GTCGAATTGG CTGACTCGAA 60 TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 44913 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTACAATCT ATCGACTAAA AAAAAATCTC AACAATGACA GATAAAAATT ATTTTTCAGA 120 AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 44853 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATACCCCCT GGATTTACCA AATGTGTGCC TAGAAACATT GAAACTCCAA CAATTTATTC 180 TCGTCAGAGT GAGAAATTTA TCGATGATTT CGAAAGAGGT AGACAACAAC TCCCTCAGAT 44793 |||| ||||| |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| TCGTGAGAGT GAGAAATTTA TCGATGATTT CAAAAGAGGT AGACAACAAC TCCCTCAGAT 240 ACCAGTTTCA GCAGAAGGTA AGCTTCAATT TTGCCTTTTT TCGTGTTTAT GAGTTTTAAA 44733 |||||||||| ||||||| ACCAGTTTCA GCAGAAG... .......... .......... .......... .......... 257 TTCTAAAATA AAAAACTCAA TTCACTTGAT TTTAAATAAA TTATTTTATT AAATTATTAA 44673 .......... .......... .......... .......... .......... .......... 257 AATTATTGAG TTTTGTCAGA CCTGTAACTG TAACTATGGC TCCAGCCCCT GTCCCTACTA 44613 .......... .......... .......... .......... .......... .......... 257 CCCACTTTTG CTTCACTGCT ATTAGTTTTT CTTTTATGTA AAAAGTACTG GAACTTTAAT 44553 | | ||| .......... .......... .......... .......... .......... CTTCATCAAT 267 CGAAATTTCA GAGACATAGA CATAAAAATT ATTAAGTATA TACCTCTGAA CTCAGTTTTT 44493 | ||| || | | || | ||| || | GGCAATGCCA -ACA-AT-GC CATTGGGATC GAGTA..... .......... .......... 299 TCTTGTAATT TTATATATCT TGTGAATTAT GTGACATATT TCATAACTCC ACGCAGTTGA 44433 | | .......... .......... .......... .......... .......... ......TAAA 303 GACGCCTGAA ATATATTTGA AAGTAAAAAA AATGTATAAA ATTACAAAAA GAAAATGTGT 44373 || ||| | ||| || | | || || || TACTCCTCTA ATA-ATCAAA GATGAAGAAG AAC....... .......... .......... 335 TCAAGGACCG TAATTTAGTT ACCTTTATCC CTATTTTTAT TACATTATGG GAGGAGAGGG 44313 .......... .......... .......... .......... .......... .......... 335 AAAGGGTAAA ATAAGGAGCG CACCAAGTGA ACTAGCCCTC GATTTCGAGG TTTAGTGTTG 44253 .......... .......... .......... .......... .......... .......... 335 GTTCGGGTTC AATTGAATTC ATAATGTTAA AGTTTTGATA TTATTGATTT TGAAACAAAG 44193 .......... .......... .......... .......... .......... .......... 335 TATTGAAAAA TACTAAATCT TTTTGATATT GTTGATTTTG AAGCTTCATC AATGGCAATG 44133 .......... .......... .......... .......... .......... .......... 335 CCAACAATGC CATTGGGATC GAGTATAAAT ACTCTAATAA TCAAAGATGA AAAGATAAAT 44073 | | |||||||| |||||||||| .......... .......... .......... .........A T-AAAGATGA AAAGATAAAT 355 CTGAATCCGT TGACATTACT GAGTCCACCA CCAAATCAAC GTGTTCGGAT TAATGATTTT 44013 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| ||| CTGAATCCGG TGACATTACT GAGTCCACCA CCAAATCAAC GTGTTCGGAT TAATGAGTTT 415 TCATCTCCTC ACATCAACGA GGAATCGAAG ACTATAATCT CTCAGGTCAA ATCAGTTTTG 43953 ||||| ||| |||||||||| |||||||||| |||||||||| ||||||||| ||||| | || GCATCTGCTC ACATCAACGA GGAATCGAAG ACTATAATCT CTCAGGTCAG ATCAGATCTG 475 GTGTTCCAGC TCACCTCTGG AAGCACGAAA AACATTGAAG CAATGATTAA GTTCGCGAAC 43893 |||||||||| |||||||||| ||||||||| ||||||||| |||||||||| |||||||||| GTGTTCCAGC TCACCTCTGG AAGCACGAAT TACATTGAAG CAATGATTAA GTTCGCGAAC 535 AATGCATTC 43884 ||||||||| AATGCATTC 544 hqPGS_C08HBa0251H04.1-_SGN-E314578+ (45032 44776,44562 44518,44436 44400,44093 43884) ******************************************************************************** EST sequence 132 +strand 388 n (File: SGN-E239441+) 1 TTTTTTTCGT GTTTCTACAA ACCACCAAAC CATTAACTTT TTTATCATTA GCTCCATTTT 61 TAATCACTCT TGAACCTCTT TGAACTAATT TTTCATGTAT TTTGAGAATC CGCAGCAGAA 121 GGAAGCTAAT CAATGTCAAA AACAATTGAA ATTCCACCTG GGTTTACAAA AACATTGTGC 181 AAAAGAATAA GTATTGATCA CGATAGTTAC ATGCAAGATG ATAAGAGGAA AATTGGGTCT 241 GTTAATGAAC TTTCTGGTAG AAGGTGCAGT TTAAGAGAAA GATTTGCCCC AAATCTGTAT 301 ATTCCTGTCC AGTTTCAGCC CCGTCCACGT ACACCGGGCC GCCCTCGGAA ACAGAGATCA 361 GATGTGACAG CTTCACAATC GTCACGAC Predicted gene structure (within gDNA segment 50558 to 48743): Exon 1 49958 49593 ( 366 n); cDNA 1 364 ( 364 n); score: 0.995 MATCH C08HBa0251H04.1- SGN-E239441+ 0.995 366 0.943 C PGS_C08HBa0251H04.1-_SGN-E239441+ (49958 49593) Alignment (genomic DNA sequence = upper lines): TTTTTTTCGT GTTTCTACAA ACCACCAAAC CATTAACTTT TTTTTATCAT TAGCTCCATT 49899 |||||||||| |||||||||| |||||||||| ||||||| | |||||||||| |||||||||| TTTTTTTCGT GTTTCTACAA ACCACCAAAC CATTAAC--T TTTTTATCAT TAGCTCCATT 58 TTTAATCACT CTTGAACCTC TTTGAACTAA TTTTTCATGT ATTTTGAGAA TCCGCAGCAG 49839 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTAATCACT CTTGAACCTC TTTGAACTAA TTTTTCATGT ATTTTGAGAA TCCGCAGCAG 118 AAGGAAGCTA ATCAATGTCA AAAACAATTG AAATTCCACC TGGGTTTACA AAAACATTGT 49779 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGAAGCTA ATCAATGTCA AAAACAATTG AAATTCCACC TGGGTTTACA AAAACATTGT 178 GCAAAAGAAT AAGTATTGAT CACGATAGTT ACATGCAAGA TGATAAGAGG AAAATTGGGT 49719 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCAAAAGAAT AAGTATTGAT CACGATAGTT ACATGCAAGA TGATAAGAGG AAAATTGGGT 238 CTGTTAATGA ACTTTCTGGT AGAAGGTGCA GTTTAAGAGA AAGATTTGCC CCAAATCTGT 49659 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTAATGA ACTTTCTGGT AGAAGGTGCA GTTTAAGAGA AAGATTTGCC CCAAATCTGT 298 ATATTCCTGT CCAGTTTCAG CCCCGTCCAC GTACACCGGG CCGCCCTCGG AAACAGAGAT 49599 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATATTCCTGT CCAGTTTCAG CCCCGTCCAC GTACACCGGG CCGCCCTCGG AAACAGAGAT 358 CAGATG 49593 |||||| CAGATG 364 hqPGS_C08HBa0251H04.1-_SGN-E239441+ (49958 49593) ******************************************************************************** EST sequence 134 +strand 583 n (File: SGN-E369675+) 1 TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 61 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG ACTAAAAACG 121 CCCTTGAGAG ATCAATTAAC ATTTGTGAGA AATCTAACCA GACCAATGCA AGCGCGTTAT 181 GTATATTCTG CTCACATGAT GGTGTGAACC AGAAGCTTTT GACATGTTCT ACTTGCAATG 241 ACAGCTTCCA CTTGAAGTGC ATAGGATGGT CACCAGGAGA TGCCAATGAT TCGAAAGTTT 301 TCATTTGTCC TTATTGTCAC TTCATGAACA GTGGAAAAAT ATCAAGAAAT GGAAGTGATC 361 CTCTGAATAT TGGTAGGAAG AGTTTCAAGT TGCACAAGCT CGTTGAGCTC CTATCTGACG 421 CTGAAGATCT ATGTTTATGG ATACAGGAAA GAGCTGTACT TCATCAAATT GGTCAAAAAG 481 CTCTCGATTT CAAAGCTCGC ATAGAAGAGA TTGTCAAGTT TGTTTTAGCT TATCTAGATG 541 AAGACCTTAG CATTATTTGC AAAAAAATTT CTGTGTTGCT TTT Predicted gene structure (within gDNA segment 57415 to 52893): Exon 1 56815 56706 ( 110 n); cDNA 1 110 ( 110 n); score: 1.000 Intron 1 56705 56601 ( 105 n); Pd: 0.992 (s: 1.00), Pa: 0.919 (s: 1.00) Exon 2 56600 56467 ( 134 n); cDNA 111 244 ( 134 n); score: 1.000 Intron 2 56466 54863 (1604 n); Pd: 1.000 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 54862 54742 ( 121 n); cDNA 245 365 ( 121 n); score: 1.000 Intron 3 54741 54659 ( 83 n); Pd: 0.900 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 54658 54585 ( 74 n); cDNA 366 439 ( 74 n); score: 1.000 Intron 4 54584 53881 ( 704 n); Pd: 0.949 (s: 1.00), Pa: 0.950 (s: 1.00) Exon 5 53880 53740 ( 141 n); cDNA 440 582 ( 143 n); score: 0.965 MATCH C08HBa0251H04.1- SGN-E369675+ 0.991 580 0.995 C PGS_C08HBa0251H04.1-_SGN-E369675+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53740) Alignment (genomic DNA sequence = upper lines): TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 56756 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 60 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG GTTTAAAGCT 56696 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG .......... 110 GTTTTCTCTT TTTATTTTGT TCCCATGGAG CTTTGTTGAC CTGGTTATAT AGCTATTATG 56636 .......... .......... .......... .......... .......... .......... 110 GGGTAGATGT GTGATGCAAT CTAATGGAAC TGCAGACTAA AAACGCCCTT GAGAGATCAA 56576 ||||| |||||||||| |||||||||| .......... .......... .......... .....ACTAA AAACGCCCTT GAGAGATCAA 135 TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 56516 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 195 ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAGG TACTGTATCT 56456 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAG. .......... 244 GGCTAATTAA CTTACTACTT TTGTGTTTCT TAAAAAGTTT TGAGATAGAT GTGTGGTACA 56396 .......... .......... .......... .......... .......... .......... 244 GGGGATTTTG AATGATCATT TGTTATTACC AGTTATCCAG AGGGTTAATA ATACTTAGAG 56336 .......... .......... .......... .......... .......... .......... 244 CCTATATGGT CAAACTTCCA AAAACATTTT ATTTTCAGAA GTACTTTTGG AGAGTACTAG 56276 .......... .......... .......... .......... .......... .......... 244 TAGTTTGTGT ATGGTCAGTT CATTTGAAAG TGTTTTTTCC ATTACTTGAT AAACATATTT 56216 .......... .......... .......... .......... .......... .......... 244 TGGTTGGTCA ATCTCTCCAA GAAGTGTTCT GACTGTGAAA TTACCCAAAA AAGACGTCAT 56156 .......... .......... .......... .......... .......... .......... 244 CAAGGGTCAT TTAGTTAGCA CTGTTAAAAT TGAGAAATGA TTTTAAATAT TTATCATGCA 56096 .......... .......... .......... .......... .......... .......... 244 AAAATTTTAT CTATGCTTTT TATGTTTATT AATTAAAGTA AAAAACATAA ATAATAAAGA 56036 .......... .......... .......... .......... .......... .......... 244 TATACTTAAA ATTTTAATCA ATATAAACAT AGTTAAAGGA GCAATAATAT TGAATATCTT 55976 .......... .......... .......... .......... .......... .......... 244 GTATAATTTT TTATTTTTTT TACATGATTT GAATAAATCA AAATACATCA TTAAATATAA 55916 .......... .......... .......... .......... .......... .......... 244 AATAAAATTC CACTCTAGTA GGAGAAGAAC CGGTAGGAAT AAGAAAGGAA AAGGGGAAGA 55856 .......... .......... .......... .......... .......... .......... 244 GGGTTGTACT CATATTTGAG AACAGATGAG TTGAGTTGAT ATCAAATAGT TTGGTAAATG 55796 .......... .......... .......... .......... .......... .......... 244 TGATCTTTTG ACAAGGATAT GTTTGTCGAG AATGCCCACA TTCAACAGAT GAATGTAGCG 55736 .......... .......... .......... .......... .......... .......... 244 GAGATGGAAA TACTCATATG GATGTGTGGG CATACAGGGG AGATATGATT AGAAACGAAG 55676 .......... .......... .......... .......... .......... .......... 244 TTATACGTGA CAAGGTGGGA GTGACTTAGT GGCAGACAAG ATGAGTGAAG CGAGACCGAG 55616 .......... .......... .......... .......... .......... .......... 244 TCACTGAGAT GGTTCCGGTA TGTGAAGAGA AGGTGCTGGA TGCGCTAGTA AGGAGGTGTG 55556 .......... .......... .......... .......... .......... .......... 244 AGAGGTTGGT TGTAGCAGGA GCTAGGAGAG GTAGAGGTAG GCTGTAGAAA AATTGTAGGA 55496 .......... .......... .......... .......... .......... .......... 244 AGATGATAAG ATAAAATATG ATTCAACTCC AACTTTTGAG GAAATGACCC TAGATAGGAA 55436 .......... .......... .......... .......... .......... .......... 244 GATATGGAGG TCAATAATTA GGGTAGATGA TTAGTAAGTA GTTGAGTATT GTCGCATTTT 55376 .......... .......... .......... .......... .......... .......... 244 ATGTGGAAGA AGCAGAGAGA TAGCCTCCTC TCCATAGTGA TCATGTAGTT CCTTATTCTT 55316 .......... .......... .......... .......... .......... .......... 244 TCATGTATGC TACTATCCGT TGCTTCATTT GCTTTGTATT TTACTATTTT GCTGTCATAT 55256 .......... .......... .......... .......... .......... .......... 244 TTTTCAACCT CTTTTGAATT TTTTTCTATT GAGCCGAGGG TTTACGTGAA ACAACATCTC 55196 .......... .......... .......... .......... .......... .......... 244 TACCCACAAA AGTAACGGTA AGTTCTATGT ACACCCAACC TCCCTAGACC CCATTTGTGG 55136 .......... .......... .......... .......... .......... .......... 244 GATTATTCTG GGTATGTTGC TGCTGCTGCT TTTATTTTTG TAGCTTCTTT CTCTACAACA 55076 .......... .......... .......... .......... .......... .......... 244 GAGAATCTGC TTCTAATACT TCTCAAAATC ACTTTTTCTT CTTGGACCTT AAATCTTAAA 55016 .......... .......... .......... .......... .......... .......... 244 AGTGCTTTTA GATTCCAAGG AACTTGTCCA AATGGGTTAT TAATTTCCTT TAATCGAGGG 54956 .......... .......... .......... .......... .......... .......... 244 ACTATTTTGT CGTTGAACAA CTATGTAGTC ATTAATTTTC ATCTGAAGAT TGCTTTCGTT 54896 .......... .......... .......... .......... .......... .......... 244 ATCACTTATA AAAGATGTTA TGCTTTGATA CAGCTTCCAC TTGAAGTGCA TAGGATGGTC 54836 ||||||| |||||||||| |||||||||| .......... .......... .......... ...CTTCCAC TTGAAGTGCA TAGGATGGTC 271 ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 54776 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 331 TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTGGTATGC TTGACCCAGT AGCGCTGTGA 54716 |||||||||| |||||||||| |||||||||| |||| TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTG...... .......... .......... 365 ATGAGCCTGC ACTTACAGTT TGTATTTATT CGTGATTTCT TGTTTTACTT ATTGCAGAAT 54656 ||| .......... .......... .......... .......... .......... .......AAT 368 ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 54596 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 428 CTATGTTTAT GGTAATGACT ATAGCCTGTT TGAATTGAAA TTTGTGCTTA GAAGACTCTA 54536 |||||||||| | CTATGTTTAT G......... .......... .......... .......... .......... 439 GACTGCTTTT AGGATGCTAG GATATCAATT ATCTTTTCTA TTGTTTCTTT GACTTCTCAT 54476 .......... .......... .......... .......... .......... .......... 439 TTTGGTTGGG ACAATGTGGA GTTCTGGCAG TGAGTGGATG TTGTGTTAGG CAGCATTTGG 54416 .......... .......... .......... .......... .......... .......... 439 TATCCCTCCT TTTATTAGTA GTTTGTAACT CTGATTTTAG AGTTGTATGA TAGAAATTCT 54356 .......... .......... .......... .......... .......... .......... 439 AGAAAACGGG AAAATTACCA TTTGAGACTA AACAAACGAC TCTAGGAATT TGTGTGTATG 54296 .......... .......... .......... .......... .......... .......... 439 GATTTTCAAT TTGTTTTCTC CTGTATTATT TCGACTTAGG TAGAAGGTAG TTGAAAGGAG 54236 .......... .......... .......... .......... .......... .......... 439 TACTGAGTGA TTTTCTGATG TTTTCAAGTG TCTTAACAGA ATATCCAATT AAATCTGAAT 54176 .......... .......... .......... .......... .......... .......... 439 GGATTGATGA AATTTGTGCA GTGGAGACAT TGCATATATC CTGAGCATTA GCAAAGCTTA 54116 .......... .......... .......... .......... .......... .......... 439 TTGACAATAG ATGGCTTATG TTAGCTTCCT TCTCTGCCCA TTCCCCTCCC CAAAAAAAGG 54056 .......... .......... .......... .......... .......... .......... 439 AACCACCTTC CATCTTTTTG TTCTGTTGTG GCGGCGTGGG GTTTCTTTTT TTGCTTCTTT 53996 .......... .......... .......... .......... .......... .......... 439 CAACTTCATT GTGCTTCTAT TGTGTGTAAC ACTTGCTCTC ACAAATTATA TGTGTCCAAA 53936 .......... .......... .......... .......... .......... .......... 439 CCGACGCTCA CTTTCTCTGA AGTGTTTTAC TTAATCATTG ATACTCTCCT GGCAGGATAC 53876 ||||| .......... .......... .......... .......... .......... .....GATAC 444 AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 53816 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 504 AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT A-TTGCAAAA 53757 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | |||||||| AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT ATTTGCAAAA 564 AAA-TTCTGT GTTGCTTT 53740 ||| |||||| |||||||| AAATTTCTGT GTTGCTTT 582 hqPGS_C08HBa0251H04.1-_SGN-E369675+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53740) ******************************************************************************** EST sequence 125 +strand 568 n (File: SGN-E259277+) 1 TGGACATGGT TTTCTCTGAG GTTCATTAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 61 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG ACTAAAAACG 121 CCCTTGAGAG ATCAATTAAC ATTTGTGAGA AATCTAACCA GACCAATGCA AGCGCGTTAT 181 GTATATTCTG CTCACATGAT GGTGTGAACC AGAAGCTTTT GACATGTTCT ACTTGCAATG 241 ACAGCTTCCA CTTGAAGTGC ATAGGATGGT CACCAGGAGA TGCCAATGAT TCGAAAGTTT 301 TCATTTGTCC TTATTGTCAC TTCATGAACA GTGGAAAAAT ATCAAGAAAT GGAAGTGATC 361 CTCTGAATAT TGGTAGGAAG AGTTTCAAGT TGCACAAGCT CGTTGAGCTC CTATCTGACG 421 CTGAAGATCT ATGTTTATGG ATACAGGAAA GAGCTGTACT TCATCAAATT GGTCAAAAAG 481 CTCTCGATTT CAAAGCTCGC ATAGAAGAGA TTGTCAAGTT TGTTTTAGCT TATCTAGATG 541 AAGACCTTAG CATTATTGCA AAAAAATT Predicted gene structure (within gDNA segment 57415 to 53142): Exon 1 56815 56706 ( 110 n); cDNA 1 110 ( 110 n); score: 0.991 Intron 1 56705 56601 ( 105 n); Pd: 0.992 (s: 1.00), Pa: 0.919 (s: 1.00) Exon 2 56600 56467 ( 134 n); cDNA 111 244 ( 134 n); score: 1.000 Intron 2 56466 54863 (1604 n); Pd: 1.000 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 54862 54742 ( 121 n); cDNA 245 365 ( 121 n); score: 1.000 Intron 3 54741 54659 ( 83 n); Pd: 0.900 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 54658 54585 ( 74 n); cDNA 366 439 ( 74 n); score: 1.000 Intron 4 54584 53881 ( 704 n); Pd: 0.949 (s: 1.00), Pa: 0.950 (s: 1.00) Exon 5 53880 53752 ( 129 n); cDNA 440 568 ( 129 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E259277+ 0.998 568 1.000 C PGS_C08HBa0251H04.1-_SGN-E259277+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53752) Alignment (genomic DNA sequence = upper lines): TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 56756 |||||||||| |||||||||| |||||| ||| |||||||||| |||||||||| |||||||||| TGGACATGGT TTTCTCTGAG GTTCATTAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 60 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG GTTTAAAGCT 56696 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG .......... 110 GTTTTCTCTT TTTATTTTGT TCCCATGGAG CTTTGTTGAC CTGGTTATAT AGCTATTATG 56636 .......... .......... .......... .......... .......... .......... 110 GGGTAGATGT GTGATGCAAT CTAATGGAAC TGCAGACTAA AAACGCCCTT GAGAGATCAA 56576 ||||| |||||||||| |||||||||| .......... .......... .......... .....ACTAA AAACGCCCTT GAGAGATCAA 135 TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 56516 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 195 ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAGG TACTGTATCT 56456 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAG. .......... 244 GGCTAATTAA CTTACTACTT TTGTGTTTCT TAAAAAGTTT TGAGATAGAT GTGTGGTACA 56396 .......... .......... .......... .......... .......... .......... 244 GGGGATTTTG AATGATCATT TGTTATTACC AGTTATCCAG AGGGTTAATA ATACTTAGAG 56336 .......... .......... .......... .......... .......... .......... 244 CCTATATGGT CAAACTTCCA AAAACATTTT ATTTTCAGAA GTACTTTTGG AGAGTACTAG 56276 .......... .......... .......... .......... .......... .......... 244 TAGTTTGTGT ATGGTCAGTT CATTTGAAAG TGTTTTTTCC ATTACTTGAT AAACATATTT 56216 .......... .......... .......... .......... .......... .......... 244 TGGTTGGTCA ATCTCTCCAA GAAGTGTTCT GACTGTGAAA TTACCCAAAA AAGACGTCAT 56156 .......... .......... .......... .......... .......... .......... 244 CAAGGGTCAT TTAGTTAGCA CTGTTAAAAT TGAGAAATGA TTTTAAATAT TTATCATGCA 56096 .......... .......... .......... .......... .......... .......... 244 AAAATTTTAT CTATGCTTTT TATGTTTATT AATTAAAGTA AAAAACATAA ATAATAAAGA 56036 .......... .......... .......... .......... .......... .......... 244 TATACTTAAA ATTTTAATCA ATATAAACAT AGTTAAAGGA GCAATAATAT TGAATATCTT 55976 .......... .......... .......... .......... .......... .......... 244 GTATAATTTT TTATTTTTTT TACATGATTT GAATAAATCA AAATACATCA TTAAATATAA 55916 .......... .......... .......... .......... .......... .......... 244 AATAAAATTC CACTCTAGTA GGAGAAGAAC CGGTAGGAAT AAGAAAGGAA AAGGGGAAGA 55856 .......... .......... .......... .......... .......... .......... 244 GGGTTGTACT CATATTTGAG AACAGATGAG TTGAGTTGAT ATCAAATAGT TTGGTAAATG 55796 .......... .......... .......... .......... .......... .......... 244 TGATCTTTTG ACAAGGATAT GTTTGTCGAG AATGCCCACA TTCAACAGAT GAATGTAGCG 55736 .......... .......... .......... .......... .......... .......... 244 GAGATGGAAA TACTCATATG GATGTGTGGG CATACAGGGG AGATATGATT AGAAACGAAG 55676 .......... .......... .......... .......... .......... .......... 244 TTATACGTGA CAAGGTGGGA GTGACTTAGT GGCAGACAAG ATGAGTGAAG CGAGACCGAG 55616 .......... .......... .......... .......... .......... .......... 244 TCACTGAGAT GGTTCCGGTA TGTGAAGAGA AGGTGCTGGA TGCGCTAGTA AGGAGGTGTG 55556 .......... .......... .......... .......... .......... .......... 244 AGAGGTTGGT TGTAGCAGGA GCTAGGAGAG GTAGAGGTAG GCTGTAGAAA AATTGTAGGA 55496 .......... .......... .......... .......... .......... .......... 244 AGATGATAAG ATAAAATATG ATTCAACTCC AACTTTTGAG GAAATGACCC TAGATAGGAA 55436 .......... .......... .......... .......... .......... .......... 244 GATATGGAGG TCAATAATTA GGGTAGATGA TTAGTAAGTA GTTGAGTATT GTCGCATTTT 55376 .......... .......... .......... .......... .......... .......... 244 ATGTGGAAGA AGCAGAGAGA TAGCCTCCTC TCCATAGTGA TCATGTAGTT CCTTATTCTT 55316 .......... .......... .......... .......... .......... .......... 244 TCATGTATGC TACTATCCGT TGCTTCATTT GCTTTGTATT TTACTATTTT GCTGTCATAT 55256 .......... .......... .......... .......... .......... .......... 244 TTTTCAACCT CTTTTGAATT TTTTTCTATT GAGCCGAGGG TTTACGTGAA ACAACATCTC 55196 .......... .......... .......... .......... .......... .......... 244 TACCCACAAA AGTAACGGTA AGTTCTATGT ACACCCAACC TCCCTAGACC CCATTTGTGG 55136 .......... .......... .......... .......... .......... .......... 244 GATTATTCTG GGTATGTTGC TGCTGCTGCT TTTATTTTTG TAGCTTCTTT CTCTACAACA 55076 .......... .......... .......... .......... .......... .......... 244 GAGAATCTGC TTCTAATACT TCTCAAAATC ACTTTTTCTT CTTGGACCTT AAATCTTAAA 55016 .......... .......... .......... .......... .......... .......... 244 AGTGCTTTTA GATTCCAAGG AACTTGTCCA AATGGGTTAT TAATTTCCTT TAATCGAGGG 54956 .......... .......... .......... .......... .......... .......... 244 ACTATTTTGT CGTTGAACAA CTATGTAGTC ATTAATTTTC ATCTGAAGAT TGCTTTCGTT 54896 .......... .......... .......... .......... .......... .......... 244 ATCACTTATA AAAGATGTTA TGCTTTGATA CAGCTTCCAC TTGAAGTGCA TAGGATGGTC 54836 ||||||| |||||||||| |||||||||| .......... .......... .......... ...CTTCCAC TTGAAGTGCA TAGGATGGTC 271 ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 54776 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 331 TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTGGTATGC TTGACCCAGT AGCGCTGTGA 54716 |||||||||| |||||||||| |||||||||| |||| TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTG...... .......... .......... 365 ATGAGCCTGC ACTTACAGTT TGTATTTATT CGTGATTTCT TGTTTTACTT ATTGCAGAAT 54656 ||| .......... .......... .......... .......... .......... .......AAT 368 ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 54596 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 428 CTATGTTTAT GGTAATGACT ATAGCCTGTT TGAATTGAAA TTTGTGCTTA GAAGACTCTA 54536 |||||||||| | CTATGTTTAT G......... .......... .......... .......... .......... 439 GACTGCTTTT AGGATGCTAG GATATCAATT ATCTTTTCTA TTGTTTCTTT GACTTCTCAT 54476 .......... .......... .......... .......... .......... .......... 439 TTTGGTTGGG ACAATGTGGA GTTCTGGCAG TGAGTGGATG TTGTGTTAGG CAGCATTTGG 54416 .......... .......... .......... .......... .......... .......... 439 TATCCCTCCT TTTATTAGTA GTTTGTAACT CTGATTTTAG AGTTGTATGA TAGAAATTCT 54356 .......... .......... .......... .......... .......... .......... 439 AGAAAACGGG AAAATTACCA TTTGAGACTA AACAAACGAC TCTAGGAATT TGTGTGTATG 54296 .......... .......... .......... .......... .......... .......... 439 GATTTTCAAT TTGTTTTCTC CTGTATTATT TCGACTTAGG TAGAAGGTAG TTGAAAGGAG 54236 .......... .......... .......... .......... .......... .......... 439 TACTGAGTGA TTTTCTGATG TTTTCAAGTG TCTTAACAGA ATATCCAATT AAATCTGAAT 54176 .......... .......... .......... .......... .......... .......... 439 GGATTGATGA AATTTGTGCA GTGGAGACAT TGCATATATC CTGAGCATTA GCAAAGCTTA 54116 .......... .......... .......... .......... .......... .......... 439 TTGACAATAG ATGGCTTATG TTAGCTTCCT TCTCTGCCCA TTCCCCTCCC CAAAAAAAGG 54056 .......... .......... .......... .......... .......... .......... 439 AACCACCTTC CATCTTTTTG TTCTGTTGTG GCGGCGTGGG GTTTCTTTTT TTGCTTCTTT 53996 .......... .......... .......... .......... .......... .......... 439 CAACTTCATT GTGCTTCTAT TGTGTGTAAC ACTTGCTCTC ACAAATTATA TGTGTCCAAA 53936 .......... .......... .......... .......... .......... .......... 439 CCGACGCTCA CTTTCTCTGA AGTGTTTTAC TTAATCATTG ATACTCTCCT GGCAGGATAC 53876 ||||| .......... .......... .......... .......... .......... .....GATAC 444 AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 53816 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 504 AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT ATTGCAAAAA 53756 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT ATTGCAAAAA 564 AATT 53752 |||| AATT 568 hqPGS_C08HBa0251H04.1-_SGN-E259277+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53752) ******************************************************************************** EST sequence 104 +strand 565 n (File: SGN-E252039+) 1 TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 61 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG ACTAAAAACG 121 CCCTTGAGAG ATCAATTAAC ATTTGTGAGA AATCTAACCA GACCAATGCA AGCGCGTTAT 181 GTATATTCTG CTCACATGAT GGTGTGAACC AGAAGCTTTT GACATGTTCT ACTTGCAATG 241 ACAGCTTCCA CTTGAAGTGC ATAGGATGGT CACCAGGAGA TGCCAATGAT TCGAAAGTTT 301 TCATTTGTCC TTATTGTCAC TTCATGAACA GTGGAAAAAT ATCAAGAAAT GGAAGTGATC 361 CTCTGAATAT TGGTAGGAAG AGTTTCAAGT TGCACAAGCT CGTTGAGCTC CTATCTGACG 421 CTGAAGATCT ATGTTTATGG ATACAGGAAA GAGCTGTACT TCATCAAATT GGTCAAAAAG 481 CTCTCGATTT CAAAGCTCGC ATAGAAGAGA TTGTCAAGTT TGTTTTAGCT TATCTAGATG 541 AAGACCTTAG CATTATTGCA AAAAA Predicted gene structure (within gDNA segment 57415 to 53145): Exon 1 56815 56706 ( 110 n); cDNA 1 110 ( 110 n); score: 1.000 Intron 1 56705 56601 ( 105 n); Pd: 0.992 (s: 1.00), Pa: 0.919 (s: 1.00) Exon 2 56600 56467 ( 134 n); cDNA 111 244 ( 134 n); score: 1.000 Intron 2 56466 54863 (1604 n); Pd: 1.000 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 54862 54742 ( 121 n); cDNA 245 365 ( 121 n); score: 1.000 Intron 3 54741 54659 ( 83 n); Pd: 0.900 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 54658 54585 ( 74 n); cDNA 366 439 ( 74 n); score: 1.000 Intron 4 54584 53881 ( 704 n); Pd: 0.949 (s: 1.00), Pa: 0.950 (s: 1.00) Exon 5 53880 53755 ( 126 n); cDNA 440 565 ( 126 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E252039+ 1.000 565 1.000 C PGS_C08HBa0251H04.1-_SGN-E252039+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53755) Alignment (genomic DNA sequence = upper lines): TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 56756 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGACATGGT TTTCTCTGAG GTTCATAAGA CAGATGAGTG GAAGCGCCGG TGTGAGGAGG 60 TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG GTTTAAAGCT 56696 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTTACACCC TTCAATTAGA GATGCTAACC TGCTTGCTGC TCTTTTACAG .......... 110 GTTTTCTCTT TTTATTTTGT TCCCATGGAG CTTTGTTGAC CTGGTTATAT AGCTATTATG 56636 .......... .......... .......... .......... .......... .......... 110 GGGTAGATGT GTGATGCAAT CTAATGGAAC TGCAGACTAA AAACGCCCTT GAGAGATCAA 56576 ||||| |||||||||| |||||||||| .......... .......... .......... .....ACTAA AAACGCCCTT GAGAGATCAA 135 TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 56516 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAACATTTG TGAGAAATCT AACCAGACCA ATGCAAGCGC GTTATGTATA TTCTGCTCAC 195 ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAGG TACTGTATCT 56456 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| ATGATGGTGT GAACCAGAAG CTTTTGACAT GTTCTACTTG CAATGACAG. .......... 244 GGCTAATTAA CTTACTACTT TTGTGTTTCT TAAAAAGTTT TGAGATAGAT GTGTGGTACA 56396 .......... .......... .......... .......... .......... .......... 244 GGGGATTTTG AATGATCATT TGTTATTACC AGTTATCCAG AGGGTTAATA ATACTTAGAG 56336 .......... .......... .......... .......... .......... .......... 244 CCTATATGGT CAAACTTCCA AAAACATTTT ATTTTCAGAA GTACTTTTGG AGAGTACTAG 56276 .......... .......... .......... .......... .......... .......... 244 TAGTTTGTGT ATGGTCAGTT CATTTGAAAG TGTTTTTTCC ATTACTTGAT AAACATATTT 56216 .......... .......... .......... .......... .......... .......... 244 TGGTTGGTCA ATCTCTCCAA GAAGTGTTCT GACTGTGAAA TTACCCAAAA AAGACGTCAT 56156 .......... .......... .......... .......... .......... .......... 244 CAAGGGTCAT TTAGTTAGCA CTGTTAAAAT TGAGAAATGA TTTTAAATAT TTATCATGCA 56096 .......... .......... .......... .......... .......... .......... 244 AAAATTTTAT CTATGCTTTT TATGTTTATT AATTAAAGTA AAAAACATAA ATAATAAAGA 56036 .......... .......... .......... .......... .......... .......... 244 TATACTTAAA ATTTTAATCA ATATAAACAT AGTTAAAGGA GCAATAATAT TGAATATCTT 55976 .......... .......... .......... .......... .......... .......... 244 GTATAATTTT TTATTTTTTT TACATGATTT GAATAAATCA AAATACATCA TTAAATATAA 55916 .......... .......... .......... .......... .......... .......... 244 AATAAAATTC CACTCTAGTA GGAGAAGAAC CGGTAGGAAT AAGAAAGGAA AAGGGGAAGA 55856 .......... .......... .......... .......... .......... .......... 244 GGGTTGTACT CATATTTGAG AACAGATGAG TTGAGTTGAT ATCAAATAGT TTGGTAAATG 55796 .......... .......... .......... .......... .......... .......... 244 TGATCTTTTG ACAAGGATAT GTTTGTCGAG AATGCCCACA TTCAACAGAT GAATGTAGCG 55736 .......... .......... .......... .......... .......... .......... 244 GAGATGGAAA TACTCATATG GATGTGTGGG CATACAGGGG AGATATGATT AGAAACGAAG 55676 .......... .......... .......... .......... .......... .......... 244 TTATACGTGA CAAGGTGGGA GTGACTTAGT GGCAGACAAG ATGAGTGAAG CGAGACCGAG 55616 .......... .......... .......... .......... .......... .......... 244 TCACTGAGAT GGTTCCGGTA TGTGAAGAGA AGGTGCTGGA TGCGCTAGTA AGGAGGTGTG 55556 .......... .......... .......... .......... .......... .......... 244 AGAGGTTGGT TGTAGCAGGA GCTAGGAGAG GTAGAGGTAG GCTGTAGAAA AATTGTAGGA 55496 .......... .......... .......... .......... .......... .......... 244 AGATGATAAG ATAAAATATG ATTCAACTCC AACTTTTGAG GAAATGACCC TAGATAGGAA 55436 .......... .......... .......... .......... .......... .......... 244 GATATGGAGG TCAATAATTA GGGTAGATGA TTAGTAAGTA GTTGAGTATT GTCGCATTTT 55376 .......... .......... .......... .......... .......... .......... 244 ATGTGGAAGA AGCAGAGAGA TAGCCTCCTC TCCATAGTGA TCATGTAGTT CCTTATTCTT 55316 .......... .......... .......... .......... .......... .......... 244 TCATGTATGC TACTATCCGT TGCTTCATTT GCTTTGTATT TTACTATTTT GCTGTCATAT 55256 .......... .......... .......... .......... .......... .......... 244 TTTTCAACCT CTTTTGAATT TTTTTCTATT GAGCCGAGGG TTTACGTGAA ACAACATCTC 55196 .......... .......... .......... .......... .......... .......... 244 TACCCACAAA AGTAACGGTA AGTTCTATGT ACACCCAACC TCCCTAGACC CCATTTGTGG 55136 .......... .......... .......... .......... .......... .......... 244 GATTATTCTG GGTATGTTGC TGCTGCTGCT TTTATTTTTG TAGCTTCTTT CTCTACAACA 55076 .......... .......... .......... .......... .......... .......... 244 GAGAATCTGC TTCTAATACT TCTCAAAATC ACTTTTTCTT CTTGGACCTT AAATCTTAAA 55016 .......... .......... .......... .......... .......... .......... 244 AGTGCTTTTA GATTCCAAGG AACTTGTCCA AATGGGTTAT TAATTTCCTT TAATCGAGGG 54956 .......... .......... .......... .......... .......... .......... 244 ACTATTTTGT CGTTGAACAA CTATGTAGTC ATTAATTTTC ATCTGAAGAT TGCTTTCGTT 54896 .......... .......... .......... .......... .......... .......... 244 ATCACTTATA AAAGATGTTA TGCTTTGATA CAGCTTCCAC TTGAAGTGCA TAGGATGGTC 54836 ||||||| |||||||||| |||||||||| .......... .......... .......... ...CTTCCAC TTGAAGTGCA TAGGATGGTC 271 ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 54776 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAGGAGAT GCCAATGATT CGAAAGTTTT CATTTGTCCT TATTGTCACT TCATGAACAG 331 TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTGGTATGC TTGACCCAGT AGCGCTGTGA 54716 |||||||||| |||||||||| |||||||||| |||| TGGAAAAATA TCAAGAAATG GAAGTGATCC TCTG...... .......... .......... 365 ATGAGCCTGC ACTTACAGTT TGTATTTATT CGTGATTTCT TGTTTTACTT ATTGCAGAAT 54656 ||| .......... .......... .......... .......... .......... .......AAT 368 ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 54596 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGGTAGGA AGAGTTTCAA GTTGCACAAG CTCGTTGAGC TCCTATCTGA CGCTGAAGAT 428 CTATGTTTAT GGTAATGACT ATAGCCTGTT TGAATTGAAA TTTGTGCTTA GAAGACTCTA 54536 |||||||||| | CTATGTTTAT G......... .......... .......... .......... .......... 439 GACTGCTTTT AGGATGCTAG GATATCAATT ATCTTTTCTA TTGTTTCTTT GACTTCTCAT 54476 .......... .......... .......... .......... .......... .......... 439 TTTGGTTGGG ACAATGTGGA GTTCTGGCAG TGAGTGGATG TTGTGTTAGG CAGCATTTGG 54416 .......... .......... .......... .......... .......... .......... 439 TATCCCTCCT TTTATTAGTA GTTTGTAACT CTGATTTTAG AGTTGTATGA TAGAAATTCT 54356 .......... .......... .......... .......... .......... .......... 439 AGAAAACGGG AAAATTACCA TTTGAGACTA AACAAACGAC TCTAGGAATT TGTGTGTATG 54296 .......... .......... .......... .......... .......... .......... 439 GATTTTCAAT TTGTTTTCTC CTGTATTATT TCGACTTAGG TAGAAGGTAG TTGAAAGGAG 54236 .......... .......... .......... .......... .......... .......... 439 TACTGAGTGA TTTTCTGATG TTTTCAAGTG TCTTAACAGA ATATCCAATT AAATCTGAAT 54176 .......... .......... .......... .......... .......... .......... 439 GGATTGATGA AATTTGTGCA GTGGAGACAT TGCATATATC CTGAGCATTA GCAAAGCTTA 54116 .......... .......... .......... .......... .......... .......... 439 TTGACAATAG ATGGCTTATG TTAGCTTCCT TCTCTGCCCA TTCCCCTCCC CAAAAAAAGG 54056 .......... .......... .......... .......... .......... .......... 439 AACCACCTTC CATCTTTTTG TTCTGTTGTG GCGGCGTGGG GTTTCTTTTT TTGCTTCTTT 53996 .......... .......... .......... .......... .......... .......... 439 CAACTTCATT GTGCTTCTAT TGTGTGTAAC ACTTGCTCTC ACAAATTATA TGTGTCCAAA 53936 .......... .......... .......... .......... .......... .......... 439 CCGACGCTCA CTTTCTCTGA AGTGTTTTAC TTAATCATTG ATACTCTCCT GGCAGGATAC 53876 ||||| .......... .......... .......... .......... .......... .....GATAC 444 AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 53816 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAAAGAGC TGTACTTCAT CAAATTGGTC AAAAAGCTCT CGATTTCAAA GCTCGCATAG 504 AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT ATTGCAAAAA 53756 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGAGATTGT CAAGTTTGTT TTAGCTTATC TAGATGAAGA CCTTAGCATT ATTGCAAAAA 564 A 53755 | A 565 hqPGS_C08HBa0251H04.1-_SGN-E252039+ (56815 56706,56600 56467,54862 54742,54658 54585,53880 53755) ******************************************************************************** EST sequence 97 +strand 499 n (File: SGN-E255440+) 1 ACATGGTTTT TTCTGAGGTT CATAAGACAG ATGAGTGGAA GCGCCGGTGT GAGGAGGTCT 61 TACACCCTTC AATTAGAGAT GCTAACCTGC TTGCTGCTCT TTTACAGACT AAAAACGCCC 121 TTGAGAGATC AATTAACATT TGTGAGAAAT CTAACCAGAC CAATGCAAGC GCGTTATGTA 181 TATTCTGCTC ACATGATGGT GTGAACCAGA AGCTTTTGAC ATGTTCTACT TGCAATGACA 241 GCTTCCACTT GAAGTGCATA GGATGGTCAC CAGGAGATGC CAATGATTCG AAAGTTTTCA 301 TTTGTCCTTA TTGTCACTTC ATGAACAGTG GAAAAATATC AAGAAATGGA AGTGATCCTC 361 TGAATATTGG TAGGAAGAGT TTCAAGTTGC ACAAGCTCGT TGAGCTCCTA TCTGACGCTG 421 AAGATCTATG TTTATGGATA CAGGAAAGAG CTGTACTTCA TCAAATTGGT CAAAAAGCTC 481 TCGATTTCAA AGCTCGCAT Predicted gene structure (within gDNA segment 57511 to 53208): Exon 1 56812 56706 ( 107 n); cDNA 1 107 ( 107 n); score: 0.991 Intron 1 56705 56601 ( 105 n); Pd: 0.992 (s: 1.00), Pa: 0.919 (s: 1.00) Exon 2 56600 56467 ( 134 n); cDNA 108 241 ( 134 n); score: 1.000 Intron 2 56466 54863 (1604 n); Pd: 1.000 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 54862 54742 ( 121 n); cDNA 242 362 ( 121 n); score: 1.000 Intron 3 54741 54659 ( 83 n); Pd: 0.900 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 54658 54585 ( 74 n); cDNA 363 436 ( 74 n); score: 1.000 Intron 4 54584 53881 ( 704 n); Pd: 0.949 (s: 1.00), Pa: 0.950 (s: 1.00) Exon 5 53880 53818 ( 63 n); cDNA 437 499 ( 63 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E255440+ 0.998 499 1.000 C PGS_C08HBa0251H04.1-_SGN-E255440+ (56812 56706,56600 56467,54862 54742,54658 54585,53880 53818) Alignment (genomic DNA sequence = upper lines): ACATGGTTTT CTCTGAGGTT CATAAGACAG ATGAGTGGAA GCGCCGGTGT GAGGAGGTCT 56753 |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATGGTTTT TTCTGAGGTT CATAAGACAG ATGAGTGGAA GCGCCGGTGT GAGGAGGTCT 60 TACACCCTTC AATTAGAGAT GCTAACCTGC TTGCTGCTCT TTTACAGGTT TAAAGCTGTT 56693 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TACACCCTTC AATTAGAGAT GCTAACCTGC TTGCTGCTCT TTTACAG... .......... 107 TTCTCTTTTT ATTTTGTTCC CATGGAGCTT TGTTGACCTG GTTATATAGC TATTATGGGG 56633 .......... .......... .......... .......... .......... .......... 107 TAGATGTGTG ATGCAATCTA ATGGAACTGC AGACTAAAAA CGCCCTTGAG AGATCAATTA 56573 |||||||| |||||||||| |||||||||| .......... .......... .......... ..ACTAAAAA CGCCCTTGAG AGATCAATTA 135 ACATTTGTGA GAAATCTAAC CAGACCAATG CAAGCGCGTT ATGTATATTC TGCTCACATG 56513 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATTTGTGA GAAATCTAAC CAGACCAATG CAAGCGCGTT ATGTATATTC TGCTCACATG 195 ATGGTGTGAA CCAGAAGCTT TTGACATGTT CTACTTGCAA TGACAGGTAC TGTATCTGGC 56453 |||||||||| |||||||||| |||||||||| |||||||||| |||||| ATGGTGTGAA CCAGAAGCTT TTGACATGTT CTACTTGCAA TGACAG.... .......... 241 TAATTAACTT ACTACTTTTG TGTTTCTTAA AAAGTTTTGA GATAGATGTG TGGTACAGGG 56393 .......... .......... .......... .......... .......... .......... 241 GATTTTGAAT GATCATTTGT TATTACCAGT TATCCAGAGG GTTAATAATA CTTAGAGCCT 56333 .......... .......... .......... .......... .......... .......... 241 ATATGGTCAA ACTTCCAAAA ACATTTTATT TTCAGAAGTA CTTTTGGAGA GTACTAGTAG 56273 .......... .......... .......... .......... .......... .......... 241 TTTGTGTATG GTCAGTTCAT TTGAAAGTGT TTTTTCCATT ACTTGATAAA CATATTTTGG 56213 .......... .......... .......... .......... .......... .......... 241 TTGGTCAATC TCTCCAAGAA GTGTTCTGAC TGTGAAATTA CCCAAAAAAG ACGTCATCAA 56153 .......... .......... .......... .......... .......... .......... 241 GGGTCATTTA GTTAGCACTG TTAAAATTGA GAAATGATTT TAAATATTTA TCATGCAAAA 56093 .......... .......... .......... .......... .......... .......... 241 ATTTTATCTA TGCTTTTTAT GTTTATTAAT TAAAGTAAAA AACATAAATA ATAAAGATAT 56033 .......... .......... .......... .......... .......... .......... 241 ACTTAAAATT TTAATCAATA TAAACATAGT TAAAGGAGCA ATAATATTGA ATATCTTGTA 55973 .......... .......... .......... .......... .......... .......... 241 TAATTTTTTA TTTTTTTTAC ATGATTTGAA TAAATCAAAA TACATCATTA AATATAAAAT 55913 .......... .......... .......... .......... .......... .......... 241 AAAATTCCAC TCTAGTAGGA GAAGAACCGG TAGGAATAAG AAAGGAAAAG GGGAAGAGGG 55853 .......... .......... .......... .......... .......... .......... 241 TTGTACTCAT ATTTGAGAAC AGATGAGTTG AGTTGATATC AAATAGTTTG GTAAATGTGA 55793 .......... .......... .......... .......... .......... .......... 241 TCTTTTGACA AGGATATGTT TGTCGAGAAT GCCCACATTC AACAGATGAA TGTAGCGGAG 55733 .......... .......... .......... .......... .......... .......... 241 ATGGAAATAC TCATATGGAT GTGTGGGCAT ACAGGGGAGA TATGATTAGA AACGAAGTTA 55673 .......... .......... .......... .......... .......... .......... 241 TACGTGACAA GGTGGGAGTG ACTTAGTGGC AGACAAGATG AGTGAAGCGA GACCGAGTCA 55613 .......... .......... .......... .......... .......... .......... 241 CTGAGATGGT TCCGGTATGT GAAGAGAAGG TGCTGGATGC GCTAGTAAGG AGGTGTGAGA 55553 .......... .......... .......... .......... .......... .......... 241 GGTTGGTTGT AGCAGGAGCT AGGAGAGGTA GAGGTAGGCT GTAGAAAAAT TGTAGGAAGA 55493 .......... .......... .......... .......... .......... .......... 241 TGATAAGATA AAATATGATT CAACTCCAAC TTTTGAGGAA ATGACCCTAG ATAGGAAGAT 55433 .......... .......... .......... .......... .......... .......... 241 ATGGAGGTCA ATAATTAGGG TAGATGATTA GTAAGTAGTT GAGTATTGTC GCATTTTATG 55373 .......... .......... .......... .......... .......... .......... 241 TGGAAGAAGC AGAGAGATAG CCTCCTCTCC ATAGTGATCA TGTAGTTCCT TATTCTTTCA 55313 .......... .......... .......... .......... .......... .......... 241 TGTATGCTAC TATCCGTTGC TTCATTTGCT TTGTATTTTA CTATTTTGCT GTCATATTTT 55253 .......... .......... .......... .......... .......... .......... 241 TCAACCTCTT TTGAATTTTT TTCTATTGAG CCGAGGGTTT ACGTGAAACA ACATCTCTAC 55193 .......... .......... .......... .......... .......... .......... 241 CCACAAAAGT AACGGTAAGT TCTATGTACA CCCAACCTCC CTAGACCCCA TTTGTGGGAT 55133 .......... .......... .......... .......... .......... .......... 241 TATTCTGGGT ATGTTGCTGC TGCTGCTTTT ATTTTTGTAG CTTCTTTCTC TACAACAGAG 55073 .......... .......... .......... .......... .......... .......... 241 AATCTGCTTC TAATACTTCT CAAAATCACT TTTTCTTCTT GGACCTTAAA TCTTAAAAGT 55013 .......... .......... .......... .......... .......... .......... 241 GCTTTTAGAT TCCAAGGAAC TTGTCCAAAT GGGTTATTAA TTTCCTTTAA TCGAGGGACT 54953 .......... .......... .......... .......... .......... .......... 241 ATTTTGTCGT TGAACAACTA TGTAGTCATT AATTTTCATC TGAAGATTGC TTTCGTTATC 54893 .......... .......... .......... .......... .......... .......... 241 ACTTATAAAA GATGTTATGC TTTGATACAG CTTCCACTTG AAGTGCATAG GATGGTCACC 54833 |||||||||| |||||||||| |||||||||| .......... .......... .......... CTTCCACTTG AAGTGCATAG GATGGTCACC 271 AGGAGATGCC AATGATTCGA AAGTTTTCAT TTGTCCTTAT TGTCACTTCA TGAACAGTGG 54773 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGAGATGCC AATGATTCGA AAGTTTTCAT TTGTCCTTAT TGTCACTTCA TGAACAGTGG 331 AAAAATATCA AGAAATGGAA GTGATCCTCT GGTATGCTTG ACCCAGTAGC GCTGTGAATG 54713 |||||||||| |||||||||| |||||||||| | AAAAATATCA AGAAATGGAA GTGATCCTCT G......... .......... .......... 362 AGCCTGCACT TACAGTTTGT ATTTATTCGT GATTTCTTGT TTTACTTATT GCAGAATATT 54653 |||||| .......... .......... .......... .......... .......... ....AATATT 368 GGTAGGAAGA GTTTCAAGTT GCACAAGCTC GTTGAGCTCC TATCTGACGC TGAAGATCTA 54593 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTAGGAAGA GTTTCAAGTT GCACAAGCTC GTTGAGCTCC TATCTGACGC TGAAGATCTA 428 TGTTTATGGT AATGACTATA GCCTGTTTGA ATTGAAATTT GTGCTTAGAA GACTCTAGAC 54533 |||||||| TGTTTATG.. .......... .......... .......... .......... .......... 436 TGCTTTTAGG ATGCTAGGAT ATCAATTATC TTTTCTATTG TTTCTTTGAC TTCTCATTTT 54473 .......... .......... .......... .......... .......... .......... 436 GGTTGGGACA ATGTGGAGTT CTGGCAGTGA GTGGATGTTG TGTTAGGCAG CATTTGGTAT 54413 .......... .......... .......... .......... .......... .......... 436 CCCTCCTTTT ATTAGTAGTT TGTAACTCTG ATTTTAGAGT TGTATGATAG AAATTCTAGA 54353 .......... .......... .......... .......... .......... .......... 436 AAACGGGAAA ATTACCATTT GAGACTAAAC AAACGACTCT AGGAATTTGT GTGTATGGAT 54293 .......... .......... .......... .......... .......... .......... 436 TTTCAATTTG TTTTCTCCTG TATTATTTCG ACTTAGGTAG AAGGTAGTTG AAAGGAGTAC 54233 .......... .......... .......... .......... .......... .......... 436 TGAGTGATTT TCTGATGTTT TCAAGTGTCT TAACAGAATA TCCAATTAAA TCTGAATGGA 54173 .......... .......... .......... .......... .......... .......... 436 TTGATGAAAT TTGTGCAGTG GAGACATTGC ATATATCCTG AGCATTAGCA AAGCTTATTG 54113 .......... .......... .......... .......... .......... .......... 436 ACAATAGATG GCTTATGTTA GCTTCCTTCT CTGCCCATTC CCCTCCCCAA AAAAAGGAAC 54053 .......... .......... .......... .......... .......... .......... 436 CACCTTCCAT CTTTTTGTTC TGTTGTGGCG GCGTGGGGTT TCTTTTTTTG CTTCTTTCAA 53993 .......... .......... .......... .......... .......... .......... 436 CTTCATTGTG CTTCTATTGT GTGTAACACT TGCTCTCACA AATTATATGT GTCCAAACCG 53933 .......... .......... .......... .......... .......... .......... 436 ACGCTCACTT TCTCTGAAGT GTTTTACTTA ATCATTGATA CTCTCCTGGC AGGATACAGG 53873 |||||||| .......... .......... .......... .......... .......... ..GATACAGG 444 AAAGAGCTGT ACTTCATCAA ATTGGTCAAA AAGCTCTCGA TTTCAAAGCT CGCAT 53818 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| AAAGAGCTGT ACTTCATCAA ATTGGTCAAA AAGCTCTCGA TTTCAAAGCT CGCAT 499 hqPGS_C08HBa0251H04.1-_SGN-E255440+ (56812 56706,56600 56467,54862 54742,54658 54585,53880 53818) ******************************************************************************** EST sequence 130 +strand 572 n (File: SGN-E302151+) 1 ACCCACCTTC CTATCATGTA TACAACTTGC AGGTTGTGTA TCTCCTTACT TGATTGGGTG 61 AATTGGCTTA ATAGAGCTTT GGAAGTCTCC ATTCTATCTA CTGCTGGAAG ATCCAACTTA 121 AGTGATGCTG AAGAAGTTCT CAGACAGTAT CAGAACATTT GTGTCTCATC TCCTGCAATG 181 ATTAGTCAGC TTCAGAAGGC TATTGAGAAA CACAACTCAT GGATGGATCA GGTGCATTCA 241 TTTTTTGTCC TTAACTTTAA GGATCGATCA TGGGATCTGT TGCTGCAGCT TAAGGAAAAA 301 GGGAATAATG ATGCTTTCAG TTGCTCCGAA TTGGACATGG TTTTCTCTGA GGTTCATAAG 361 ACAGATGAGT GGAAGCGCCG GTGTGAGGAG GTCTTACACC CTTCAATTAG AGATGCTAAC 421 CTGCTTGCTG CTCTTTTACA GACTAAAAAC GCCCTTGAGA GATCAATTAA CATTTGTGAG 481 AAATCTAACC AGACCAATGC AAGCGCGTTA TGTATATTCT GCTCACATGA TGGTGTGAAC 541 CAGAAGCTTT TGACATGTTC TACTTGCAAT GA Predicted gene structure (within gDNA segment 59722 to 55860): Exon 1 59122 58970 ( 153 n); cDNA 1 153 ( 153 n); score: 0.993 Intron 1 58969 58848 ( 122 n); Pd: 0.969 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 2 58847 58786 ( 62 n); cDNA 154 215 ( 62 n); score: 1.000 Intron 2 58785 57725 (1061 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 0.98) Exon 3 57724 57646 ( 79 n); cDNA 216 294 ( 79 n); score: 0.987 Intron 3 57645 56853 ( 793 n); Pd: 0.985 (s: 0.98), Pa: 0.956 (s: 1.00) Exon 4 56852 56706 ( 147 n); cDNA 295 441 ( 147 n); score: 1.000 Intron 4 56705 56601 ( 105 n); Pd: 0.992 (s: 1.00), Pa: 0.919 (s: 1.00) Exon 5 56600 56470 ( 131 n); cDNA 442 572 ( 131 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E302151+ 0.997 572 1.000 C PGS_C08HBa0251H04.1-_SGN-E302151+ (59122 58970,58847 58786,57724 57646,56852 56706,56600 56470) Alignment (genomic DNA sequence = upper lines): ACCCACCTTC CTATCATGTA TACAACTTGC AGTTTGTGTA TCTCCTTACT TGATTGGGTG 59063 |||||||||| |||||||||| |||||||||| || ||||||| |||||||||| |||||||||| ACCCACCTTC CTATCATGTA TACAACTTGC AGGTTGTGTA TCTCCTTACT TGATTGGGTG 60 AATTGGCTTA ATAGAGCTTT GGAAGTCTCC ATTCTATCTA CTGCTGGAAG ATCCAACTTA 59003 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATTGGCTTA ATAGAGCTTT GGAAGTCTCC ATTCTATCTA CTGCTGGAAG ATCCAACTTA 120 AGTGATGCTG AAGAAGTTCT CAGACAGTAT CAGGTTTTTT TATCCATTTT CTATAGTCCT 58943 |||||||||| |||||||||| |||||||||| ||| AGTGATGCTG AAGAAGTTCT CAGACAGTAT CAG....... .......... .......... 153 TATGGGGAAT TGTTCCCTCC TTGTATGTGT TAGCTATTTT GGTATATTCT ACTTACGTGA 58883 .......... .......... .......... .......... .......... .......... 153 TTTTATATTC TATATTTGAT GCCTTTGTGC TGCAGAACAT TTGTGTCTCA TCTCCTGCAA 58823 ||||| |||||||||| |||||||||| .......... .......... .......... .....AACAT TTGTGTCTCA TCTCCTGCAA 178 TGATTAGTCA GCTTCAGAAG GCTATTGAGA AACACAAGTA AGTTACCCAC TGATACAGAT 58763 |||||||||| |||||||||| |||||||||| ||||||| TGATTAGTCA GCTTCAGAAG GCTATTGAGA AACACAA... .......... .......... 215 TTCATGTGTG TCGGCCCGTG GGGTGTGTAT CTGTTTGTCA TCACATATTC ATGATTTCAA 58703 .......... .......... .......... .......... .......... .......... 215 AAAATAGCTT CTTCGGCCAC TATCTAGTGT GGAATAGGAA AAACCTAGTT TTTATATTTA 58643 .......... .......... .......... .......... .......... .......... 215 GAAATAATAT CTCATACCAA GGTCTGATAT CTCATATTAT AGGTAAAATA TCTTATGCTT 58583 .......... .......... .......... .......... .......... .......... 215 CCTTAGTTTT CTTTTCTGAG AGGCCGTTTA GTTCCATGCT ATTTTCTACA TTCTTCCCAC 58523 .......... .......... .......... .......... .......... .......... 215 AATTTTCACT ACTTTTTGTA GTAATATTTG TTACTTTTAT TTAAGAATTA TAATTATAAT 58463 .......... .......... .......... .......... .......... .......... 215 AAGACTGTTA TCTTATTCAG TCTCTTGCTT CTACTAAAAG GTTGGGTTAG GTCGTTTGGA 58403 .......... .......... .......... .......... .......... .......... 215 TTCCATTTGT CATTTATGAA TAGTTAGGTC CGTTCTGTTT GTAGAATGTG GCATAAATCT 58343 .......... .......... .......... .......... .......... .......... 215 ACTGCACCAC CATCTTGTAC ATACCCTTTT TACCCTGCCT GGTAAAAGAA ACTAAAGGAA 58283 .......... .......... .......... .......... .......... .......... 215 AAAGTCAAGA TCAGAAAGCA ATGACAAGTG AATGAATTAC TGAGAAGATG GTTGTGTACA 58223 .......... .......... .......... .......... .......... .......... 215 GAGTTCCAAA GTAATTAGGG AAATGGCTAT TGCAGATGAA ACTATGTCTA GGTGGGGAAT 58163 .......... .......... .......... .......... .......... .......... 215 CATTTGGCAT ATTGACATCA GAAAACCCCT GTCAGACACA CTGGCACATC TATGCTTTTC 58103 .......... .......... .......... .......... .......... .......... 215 ATCTTGTTTT CTTTTCGCTT TCCTTAGTTT TATGATTTCA GATTTACCGC ATTATTTGCT 58043 .......... .......... .......... .......... .......... .......... 215 GTTGTTGCCT TCTTTTTATC CTGAAGTCCA TATAAATGTT TTCAGCTGGC CTGGAAACCA 57983 .......... .......... .......... .......... .......... .......... 215 TGGTCATAAG AAAAAATATA GTCTATATAA TGTTTCTCTA TTATTTACCG TGTTTCTTAC 57923 .......... .......... .......... .......... .......... .......... 215 TTCAGTTTAT TCCTTTTCAT ACTGCTTTGA TATGCGTTAT TTTAGCCGGT GGGTTTTCGA 57863 .......... .......... .......... .......... .......... .......... 215 AAAAAGCCTC TTTACCTCCA TGAGGTAGGG GGGCGGCTGC GTACACTCTA CCTTCCCCAG 57803 .......... .......... .......... .......... .......... .......... 215 ATCCCACCTT TGCGAGTACA TGATATGTTG TTCTTGTTAG TTTTATGATT TTATCACTTA 57743 .......... .......... .......... .......... .......... .......... 215 CAATTTTAAT TTTTGCAGCT CATGGATGGA TCAGGTGCAT TCATTTTTTG TCCTTAACTT 57683 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........CT CATGGATGGA TCAGGTGCAT TCATTTTTTG TCCTTAACTT 257 TAGGGATCGA TCATGGGATC TGTTGCTGCA GCTTAAGGTA ACATATCTTT GTGTGATGTA 57623 || ||||||| |||||||||| |||||||||| ||||||| TAAGGATCGA TCATGGGATC TGTTGCTGCA GCTTAAG... .......... .......... 294 TCTTCTTTCT TCTTCTCAAG GTCAACATAT CTTTTTTTGT TTGTGTTAGA CTAGGTTCCT 57563 .......... .......... .......... .......... .......... .......... 294 TTTCCCTCCT ACAAGTCATA GCTTTTATTT TTCTGGAAAT TTTGTTATTG GGCTACTTTT 57503 .......... .......... .......... .......... .......... .......... 294 GTTGCTTTTG GATTTCTAAC TTATGAGTTT TTATTATGCT GGAAATGGAG AAGTAGTTGA 57443 .......... .......... .......... .......... .......... .......... 294 ATGTCTCATC ATTCGTCTCT GTGCTCAGTA ACTCTTCTAT AGATTGTTAA ATTTGGTATT 57383 .......... .......... .......... .......... .......... .......... 294 TGAAATTTTT CCACCAAATG GGAGAGGGGA TAACCAATAA GATTCGTTTT CACTATATTT 57323 .......... .......... .......... .......... .......... .......... 294 TTCCTTTTTC TAAATCCTTA CTCACACCCT CATAAAATAA CAGCATTCTA AATTATCCCC 57263 .......... .......... .......... .......... .......... .......... 294 CGCTATCTCG CAACCCATTT GAACTGGAGG TATTGTTTTC TCACTTCTGA TTCAGATTCC 57203 .......... .......... .......... .......... .......... .......... 294 TACTTTCAGT TACTCTTATG GAGATTGATT TTACAGCTTG TCTGGAAATT TTCATCTTAC 57143 .......... .......... .......... .......... .......... .......... 294 TACTATGTGT AGAAATTGAG GAACCGAATG AAAATGTAGA AACACTCTAG TGGAGAAACT 57083 .......... .......... .......... .......... .......... .......... 294 TATTGAGAAT TGATGATGAG GAAATTCCCG TGGATTTGAC TCATTGATTT ATCAATTGAC 57023 .......... .......... .......... .......... .......... .......... 294 TTGTTGTCCA CAACTGTAAA CTTTTATCCA TTGAACTGAC CCAAAAAATA CTCTCAACCT 56963 .......... .......... .......... .......... .......... .......... 294 TTCCAAGGAA TTAATATCGA GTTCATGTTC TTATACGTTG AAGCTTAACA TATATTGGTA 56903 .......... .......... .......... .......... .......... .......... 294 TCATGTGATA TTCATTAATT TCATGGTGTC TGGTGATTCG AACACTTCAG GAAAAAGGGA 56843 |||||||||| .......... .......... .......... .......... .......... GAAAAAGGGA 304 ATAATGATGC TTTCAGTTGC TCCGAATTGG ACATGGTTTT CTCTGAGGTT CATAAGACAG 56783 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAATGATGC TTTCAGTTGC TCCGAATTGG ACATGGTTTT CTCTGAGGTT CATAAGACAG 364 ATGAGTGGAA GCGCCGGTGT GAGGAGGTCT TACACCCTTC AATTAGAGAT GCTAACCTGC 56723 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAGTGGAA GCGCCGGTGT GAGGAGGTCT TACACCCTTC AATTAGAGAT GCTAACCTGC 424 TTGCTGCTCT TTTACAGGTT TAAAGCTGTT TTCTCTTTTT ATTTTGTTCC CATGGAGCTT 56663 |||||||||| ||||||| TTGCTGCTCT TTTACAG... .......... .......... .......... .......... 441 TGTTGACCTG GTTATATAGC TATTATGGGG TAGATGTGTG ATGCAATCTA ATGGAACTGC 56603 .......... .......... .......... .......... .......... .......... 441 AGACTAAAAA CGCCCTTGAG AGATCAATTA ACATTTGTGA GAAATCTAAC CAGACCAATG 56543 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ..ACTAAAAA CGCCCTTGAG AGATCAATTA ACATTTGTGA GAAATCTAAC CAGACCAATG 499 CAAGCGCGTT ATGTATATTC TGCTCACATG ATGGTGTGAA CCAGAAGCTT TTGACATGTT 56483 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAGCGCGTT ATGTATATTC TGCTCACATG ATGGTGTGAA CCAGAAGCTT TTGACATGTT 559 CTACTTGCAA TGA 56470 |||||||||| ||| CTACTTGCAA TGA 572 hqPGS_C08HBa0251H04.1-_SGN-E302151+ (59122 58970,58847 58786,57724 57646,56852 56706,56600 56470) ******************************************************************************** EST sequence 106 +strand 531 n (File: SGN-E205815+) 1 GATGATGAAA TATTGAGCAG ATTTGGTAAA ATTGAAAAGC AAATTCAAGC CATAGAATCA 61 GTTGTAGAAG CAGGTCAAGG TCTTGGTTTC AAGTTTGATA TGGTTCCAAA ACTTGAAGAT 121 GCTTGTTCTA CTCTTCGTTG GTGCTTTAGG GCTCTATCTT TTGCTACTGC AATTCCAACA 181 CTTGAGGAAG TCAAGACGAA TTTGGAGATT GCGACCCACC TTCCTATCAT GTATACAACT 241 TGCAGTTTGT GTATCTCCTT ACTTGATTGG GTGAATTGGC TTAATAGAGC TTTGGAAGTC 301 TCCATTCTAT CTACTGCTGG AAGATCCAAC TTAAGTGATG CTGAAGAAGT TCTCAGACAG 361 TATCAGAACA TTTGTGTCTC ATCTCCTGCA ATGATTAGTC AGCTTCAGAA GGCTATTGAG 421 AAACACAACT CATGGATGGA TCAGGTGCAT TCATTTTTTG TCCTTAACTT TAGGGATCGA 481 TCATGGGATC TGTTGCTGCA GCTTAAGGAA AAAGGGAATA ATGATGCTTT C Predicted gene structure (within gDNA segment 60761 to 56805): Exon 1 60161 59976 ( 186 n); cDNA 1 186 ( 186 n); score: 1.000 Intron 1 59975 59150 ( 826 n); Pd: 0.991 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 2 59149 58970 ( 180 n); cDNA 187 366 ( 180 n); score: 1.000 Intron 2 58969 58848 ( 122 n); Pd: 0.969 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 3 58847 58786 ( 62 n); cDNA 367 428 ( 62 n); score: 1.000 Intron 3 58785 57725 (1061 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 4 57724 57646 ( 79 n); cDNA 429 507 ( 79 n); score: 1.000 Intron 4 57645 56853 ( 793 n); Pd: 0.985 (s: 1.00), Pa: 0.956 (s: 0) Exon 5 56852 56829 ( 24 n); cDNA 508 531 ( 24 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E205815+ 1.000 531 1.000 C PGS_C08HBa0251H04.1-_SGN-E205815+ (60161 59976,59149 58970,58847 58786,57724 57646,56852 56829) Alignment (genomic DNA sequence = upper lines): GATGATGAAA TATTGAGCAG ATTTGGTAAA ATTGAAAAGC AAATTCAAGC CATAGAATCA 60102 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATGATGAAA TATTGAGCAG ATTTGGTAAA ATTGAAAAGC AAATTCAAGC CATAGAATCA 60 GTTGTAGAAG CAGGTCAAGG TCTTGGTTTC AAGTTTGATA TGGTTCCAAA ACTTGAAGAT 60042 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTGTAGAAG CAGGTCAAGG TCTTGGTTTC AAGTTTGATA TGGTTCCAAA ACTTGAAGAT 120 GCTTGTTCTA CTCTTCGTTG GTGCTTTAGG GCTCTATCTT TTGCTACTGC AATTCCAACA 59982 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTGTTCTA CTCTTCGTTG GTGCTTTAGG GCTCTATCTT TTGCTACTGC AATTCCAACA 180 CTTGAGGTAA TTATTTGTCT CATAAGATTT CTGTTCAATT TAAAGACAGA TCTGGACAAT 59922 |||||| CTTGAG.... .......... .......... .......... .......... .......... 186 GAATTGAGGT TTTGGCTGTA CATGCACTCC TTACTATTGG GGATAATAAA TGTGATTTTA 59862 .......... .......... .......... .......... .......... .......... 186 CTAGTTTTCT TTGGCTTCTT TTGGTATCCA GTGCCTAAAT TTTTGTTCCT GGCCCCATAT 59802 .......... .......... .......... .......... .......... .......... 186 TCTGCCTCTG GTATCGCCTG TATTCGAGGA TCAACAGCTC TAATACCTTT AGCCTGTCAT 59742 .......... .......... .......... .......... .......... .......... 186 CACCATGGAA TTTAAAAATT TACAGTTTGA ATTTAGTTAT TGATGGTTAG TTATTCGGAG 59682 .......... .......... .......... .......... .......... .......... 186 CAAAATTCTC TTTTAGCCAC TTTTCAATGT TAAATGCTAT CTCTCTTCGG TTCATGTAAA 59622 .......... .......... .......... .......... .......... .......... 186 TCTTTATATA CATAAGTTTG GAAACCACTG GCGGTGGTAT AGTTGGTGCA CCACTCCTTG 59562 .......... .......... .......... .......... .......... .......... 186 TTCATTCCTT ATACTTTTTT ATGTTGGCAA ACCTCTAAGT TGTAAACAGT AAGCTATGTT 59502 .......... .......... .......... .......... .......... .......... 186 GCTGACACCC AAAACCCTTC TCCCGTTTCT CCTTCCTTCA AAATTTTGCC AGCTACCATG 59442 .......... .......... .......... .......... .......... .......... 186 TTGTTAGGAG CTGAGGACCT TTTCTTTTTA TCTTTTATGT GTTATCAGTT GTTTTTTCTT 59382 .......... .......... .......... .......... .......... .......... 186 TTTTAGCCCC TAATTTGGCA CATCAGACTT TTTGTCTTTA TGATTAAAAA AAGAACTCTT 59322 .......... .......... .......... .......... .......... .......... 186 GGTCCTATCT CTCCACATGT AAGCCTTTTA ATTCCATAGT TTGTCCTACC TCTTTGTTAT 59262 .......... .......... .......... .......... .......... .......... 186 GGAGTTGTTG CTTTTATGGT GCCCTGGTTA AGACATTTTC TTACTCTTCA TCTGCCCTCT 59202 .......... .......... .......... .......... .......... .......... 186 TAAAATATGG TACACAATAC ATGCTTAATT TTATTTGTTG ACATTGCTGT AGGAAGTCAA 59142 |||||||| .......... .......... .......... .......... .......... ..GAAGTCAA 194 GACGAATTTG GAGATTGCGA CCCACCTTCC TATCATGTAT ACAACTTGCA GTTTGTGTAT 59082 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACGAATTTG GAGATTGCGA CCCACCTTCC TATCATGTAT ACAACTTGCA GTTTGTGTAT 254 CTCCTTACTT GATTGGGTGA ATTGGCTTAA TAGAGCTTTG GAAGTCTCCA TTCTATCTAC 59022 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCTTACTT GATTGGGTGA ATTGGCTTAA TAGAGCTTTG GAAGTCTCCA TTCTATCTAC 314 TGCTGGAAGA TCCAACTTAA GTGATGCTGA AGAAGTTCTC AGACAGTATC AGGTTTTTTT 58962 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || TGCTGGAAGA TCCAACTTAA GTGATGCTGA AGAAGTTCTC AGACAGTATC AG........ 366 ATCCATTTTC TATAGTCCTT ATGGGGAATT GTTCCCTCCT TGTATGTGTT AGCTATTTTG 58902 .......... .......... .......... .......... .......... .......... 366 GTATATTCTA CTTACGTGAT TTTATATTCT ATATTTGATG CCTTTGTGCT GCAGAACATT 58842 |||||| .......... .......... .......... .......... .......... ....AACATT 372 TGTGTCTCAT CTCCTGCAAT GATTAGTCAG CTTCAGAAGG CTATTGAGAA ACACAAGTAA 58782 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| TGTGTCTCAT CTCCTGCAAT GATTAGTCAG CTTCAGAAGG CTATTGAGAA ACACAA.... 428 GTTACCCACT GATACAGATT TCATGTGTGT CGGCCCGTGG GGTGTGTATC TGTTTGTCAT 58722 .......... .......... .......... .......... .......... .......... 428 CACATATTCA TGATTTCAAA AAATAGCTTC TTCGGCCACT ATCTAGTGTG GAATAGGAAA 58662 .......... .......... .......... .......... .......... .......... 428 AACCTAGTTT TTATATTTAG AAATAATATC TCATACCAAG GTCTGATATC TCATATTATA 58602 .......... .......... .......... .......... .......... .......... 428 GGTAAAATAT CTTATGCTTC CTTAGTTTTC TTTTCTGAGA GGCCGTTTAG TTCCATGCTA 58542 .......... .......... .......... .......... .......... .......... 428 TTTTCTACAT TCTTCCCACA ATTTTCACTA CTTTTTGTAG TAATATTTGT TACTTTTATT 58482 .......... .......... .......... .......... .......... .......... 428 TAAGAATTAT AATTATAATA AGACTGTTAT CTTATTCAGT CTCTTGCTTC TACTAAAAGG 58422 .......... .......... .......... .......... .......... .......... 428 TTGGGTTAGG TCGTTTGGAT TCCATTTGTC ATTTATGAAT AGTTAGGTCC GTTCTGTTTG 58362 .......... .......... .......... .......... .......... .......... 428 TAGAATGTGG CATAAATCTA CTGCACCACC ATCTTGTACA TACCCTTTTT ACCCTGCCTG 58302 .......... .......... .......... .......... .......... .......... 428 GTAAAAGAAA CTAAAGGAAA AAGTCAAGAT CAGAAAGCAA TGACAAGTGA ATGAATTACT 58242 .......... .......... .......... .......... .......... .......... 428 GAGAAGATGG TTGTGTACAG AGTTCCAAAG TAATTAGGGA AATGGCTATT GCAGATGAAA 58182 .......... .......... .......... .......... .......... .......... 428 CTATGTCTAG GTGGGGAATC ATTTGGCATA TTGACATCAG AAAACCCCTG TCAGACACAC 58122 .......... .......... .......... .......... .......... .......... 428 TGGCACATCT ATGCTTTTCA TCTTGTTTTC TTTTCGCTTT CCTTAGTTTT ATGATTTCAG 58062 .......... .......... .......... .......... .......... .......... 428 ATTTACCGCA TTATTTGCTG TTGTTGCCTT CTTTTTATCC TGAAGTCCAT ATAAATGTTT 58002 .......... .......... .......... .......... .......... .......... 428 TCAGCTGGCC TGGAAACCAT GGTCATAAGA AAAAATATAG TCTATATAAT GTTTCTCTAT 57942 .......... .......... .......... .......... .......... .......... 428 TATTTACCGT GTTTCTTACT TCAGTTTATT CCTTTTCATA CTGCTTTGAT ATGCGTTATT 57882 .......... .......... .......... .......... .......... .......... 428 TTAGCCGGTG GGTTTTCGAA AAAAGCCTCT TTACCTCCAT GAGGTAGGGG GGCGGCTGCG 57822 .......... .......... .......... .......... .......... .......... 428 TACACTCTAC CTTCCCCAGA TCCCACCTTT GCGAGTACAT GATATGTTGT TCTTGTTAGT 57762 .......... .......... .......... .......... .......... .......... 428 TTTATGATTT TATCACTTAC AATTTTAATT TTTGCAGCTC ATGGATGGAT CAGGTGCATT 57702 ||| |||||||||| |||||||||| .......... .......... .......... .......CTC ATGGATGGAT CAGGTGCATT 451 CATTTTTTGT CCTTAACTTT AGGGATCGAT CATGGGATCT GTTGCTGCAG CTTAAGGTAA 57642 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| CATTTTTTGT CCTTAACTTT AGGGATCGAT CATGGGATCT GTTGCTGCAG CTTAAG.... 507 CATATCTTTG TGTGATGTAT CTTCTTTCTT CTTCTCAAGG TCAACATATC TTTTTTTGTT 57582 .......... .......... .......... .......... .......... .......... 507 TGTGTTAGAC TAGGTTCCTT TTCCCTCCTA CAAGTCATAG CTTTTATTTT TCTGGAAATT 57522 .......... .......... .......... .......... .......... .......... 507 TTGTTATTGG GCTACTTTTG TTGCTTTTGG ATTTCTAACT TATGAGTTTT TATTATGCTG 57462 .......... .......... .......... .......... .......... .......... 507 GAAATGGAGA AGTAGTTGAA TGTCTCATCA TTCGTCTCTG TGCTCAGTAA CTCTTCTATA 57402 .......... .......... .......... .......... .......... .......... 507 GATTGTTAAA TTTGGTATTT GAAATTTTTC CACCAAATGG GAGAGGGGAT AACCAATAAG 57342 .......... .......... .......... .......... .......... .......... 507 ATTCGTTTTC ACTATATTTT TCCTTTTTCT AAATCCTTAC TCACACCCTC ATAAAATAAC 57282 .......... .......... .......... .......... .......... .......... 507 AGCATTCTAA ATTATCCCCC GCTATCTCGC AACCCATTTG AACTGGAGGT ATTGTTTTCT 57222 .......... .......... .......... .......... .......... .......... 507 CACTTCTGAT TCAGATTCCT ACTTTCAGTT ACTCTTATGG AGATTGATTT TACAGCTTGT 57162 .......... .......... .......... .......... .......... .......... 507 CTGGAAATTT TCATCTTACT ACTATGTGTA GAAATTGAGG AACCGAATGA AAATGTAGAA 57102 .......... .......... .......... .......... .......... .......... 507 ACACTCTAGT GGAGAAACTT ATTGAGAATT GATGATGAGG AAATTCCCGT GGATTTGACT 57042 .......... .......... .......... .......... .......... .......... 507 CATTGATTTA TCAATTGACT TGTTGTCCAC AACTGTAAAC TTTTATCCAT TGAACTGACC 56982 .......... .......... .......... .......... .......... .......... 507 CAAAAAATAC TCTCAACCTT TCCAAGGAAT TAATATCGAG TTCATGTTCT TATACGTTGA 56922 .......... .......... .......... .......... .......... .......... 507 AGCTTAACAT ATATTGGTAT CATGTGATAT TCATTAATTT CATGGTGTCT GGTGATTCGA 56862 .......... .......... .......... .......... .......... .......... 507 ACACTTCAGG AAAAAGGGAA TAATGATGCT TTC 56829 | |||||||||| |||||||||| ||| .........G AAAAAGGGAA TAATGATGCT TTC 531 hqPGS_C08HBa0251H04.1-_SGN-E205815+ (60161 59976,59149 58970,58847 58786,57724 57646,56852 56829) ******************************************************************************** EST sequence 5 -strand 331 n (File: SGN-E215930-) 1 CTATCTTTGA GGAGCTTCTC TACTGAAGTT GTTTGAGAGT GCAATTTCAA TTCTGTCTTT 61 TTCCTGTGTT GTAAATGATT TCTCAATTTC ATTGAGAGTT ATATCAGCCG ATTTTTCCAT 121 CGTTAGACAA ATAATCTAGC TAGCTAGATT AAGATATGTT ACATGTATCT ATGGCTCGAA 181 AACGTCATAT GTAGCTCTCT CCCCTTGGGA TGTGATCAAT CTGGTTCCAG ATAGCATTTG 241 TATACATTTT CCCCAGAAAA GTAACATAGC AATGCTAAAG TAATAAATGA ATCTATTGGC 301 TGACCAAAAA AAACCAAAAA AAAAAAAAAA A Predicted gene structure (within gDNA segment 78852 to 77096): Exon 1 78242 77936 ( 307 n); cDNA 1 307 ( 307 n); score: 1.000 PPA cDNA 316 331 MATCH C08HBa0251H04.1- SGN-E215930- 1.000 307 0.927 C PGS_C08HBa0251H04.1-_SGN-E215930- (78242 77936) Alignment (genomic DNA sequence = upper lines): CTATCTTTGA GGAGCTTCTC TACTGAAGTT GTTTGAGAGT GCAATTTCAA TTCTGTCTTT 78183 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTATCTTTGA GGAGCTTCTC TACTGAAGTT GTTTGAGAGT GCAATTTCAA TTCTGTCTTT 60 TTCCTGTGTT GTAAATGATT TCTCAATTTC ATTGAGAGTT ATATCAGCCG ATTTTTCCAT 78123 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCCTGTGTT GTAAATGATT TCTCAATTTC ATTGAGAGTT ATATCAGCCG ATTTTTCCAT 120 CGTTAGACAA ATAATCTAGC TAGCTAGATT AAGATATGTT ACATGTATCT ATGGCTCGAA 78063 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGTTAGACAA ATAATCTAGC TAGCTAGATT AAGATATGTT ACATGTATCT ATGGCTCGAA 180 AACGTCATAT GTAGCTCTCT CCCCTTGGGA TGTGATCAAT CTGGTTCCAG ATAGCATTTG 78003 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACGTCATAT GTAGCTCTCT CCCCTTGGGA TGTGATCAAT CTGGTTCCAG ATAGCATTTG 240 TATACATTTT CCCCAGAAAA GTAACATAGC AATGCTAAAG TAATAAATGA ATCTATTGGC 77943 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATACATTTT CCCCAGAAAA GTAACATAGC AATGCTAAAG TAATAAATGA ATCTATTGGC 300 TGACCAA 77936 ||||||| TGACCAA 307 hqPGS_C08HBa0251H04.1-_SGN-E215930- (78242 77936) ******************************************************************************** EST sequence 101 +strand 616 n (File: SGN-E250603+) 1 TAAAGTTGGC TCACTTGGGC TGGATATGAT GTTTAGAACA TGCACTGTTC AGGTAAATCT 61 GGACTTCAGT TCTGAAGCTG ACATGATCAG AAAGTTCCGT GCTGGTCTTG CCTTGCAGCC 121 TATTGCTACA GCTCTATTTG CAAATTCACC TTTCACTGAA GGAAAACCTA ATGGTTATCT 181 CAGCAAGAGA AGCCACATTT GGACCGATAC AGATAATAAC CGCGCTGGGA TGCTTCCTTT 241 CGTCTTTGAT GACTCTTTTG GGTTTGAGCA ATATGTAGAT TATGCACTTG ATGTCCCAAT 301 GTATTTTGTC TATCGGAAGA AGAAGTATGT TGATTGTACT GGATTGTCTT TCCGGGACTT 361 CATGAATGGA AAACTTCCGC CCATTCCCGG CGAATACCCT ACTCTTAATG ATTGGGAGAA 421 TCATCTCACA ACAATATTTC CTGAGGTCAG ACTCAAAAGA TATCTGGAAA TGAGAGGTGC 481 TGATGGAGGG CCTTGGAGAC GGTTATGCGC ATTGCCTGCA TTCTGGGTGG GTATACTCTA 541 CGATGAGGGG TCTTTGCAAA GCGTTTTGGA CATGACATTT GATTGGACTG CAGAAGAAAG 601 AGACATGTTA AGGAAT Predicted gene structure (within gDNA segment 82067 to 78674): Exon 1 81467 81416 ( 52 n); cDNA 1 52 ( 52 n); score: 1.000 Intron 1 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 81203 81135 ( 69 n); cDNA 53 121 ( 69 n); score: 1.000 Intron 2 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 3 81055 80985 ( 71 n); cDNA 122 192 ( 71 n); score: 1.000 Intron 3 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 4 80900 80832 ( 69 n); cDNA 193 261 ( 69 n); score: 1.000 Intron 4 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 5 80038 79945 ( 94 n); cDNA 262 355 ( 94 n); score: 1.000 Intron 5 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 6 79873 79784 ( 90 n); cDNA 356 445 ( 90 n); score: 1.000 Intron 6 79783 79695 ( 89 n); Pd: 0.999 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 7 79694 79614 ( 81 n); cDNA 446 526 ( 81 n); score: 1.000 Intron 7 79613 79374 ( 240 n); Pd: 0.993 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 8 79373 79284 ( 90 n); cDNA 527 616 ( 90 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E250603+ 1.000 616 1.000 C PGS_C08HBa0251H04.1-_SGN-E250603+ (81467 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79614,79373 79284) Alignment (genomic DNA sequence = upper lines): TAAAGTTGGC TCACTTGGGC TGGATATGAT GTTTAGAACA TGCACTGTTC AGGTAAGCTG 81408 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| || TAAAGTTGGC TCACTTGGGC TGGATATGAT GTTTAGAACA TGCACTGTTC AG........ 52 AATGGTTTAG CTTTTTTCTT TTCATTTCGT TCCATTTTTT TGATGGGATG CCATTGCCCC 81348 .......... .......... .......... .......... .......... .......... 52 TTGACTATTG GAGTGCATAC CCCTGTGCAG CATTCAAAGC TATATTTTTT TTTCCTGCCC 81288 .......... .......... .......... .......... .......... .......... 52 ATTTGTGTCT GGGTTCCCGT CATGCCCTGC ATGGTAACAT TCATTAGTAA GTTGAATCAA 81228 .......... .......... .......... .......... .......... .......... 52 TATTTACGAA TATGTTGTTG ACAGGTAAAT CTGGACTTCA GTTCTGAAGC TGACATGATC 81168 |||||| |||||||||| |||||||||| |||||||||| .......... .......... ....GTAAAT CTGGACTTCA GTTCTGAAGC TGACATGATC 88 AGAAAGTTCC GTGCTGGTCT TGCCTTGCAG CCTGTATGGT ACCTTTATCC TTTCTGTCCT 81108 |||||||||| |||||||||| |||||||||| ||| AGAAAGTTCC GTGCTGGTCT TGCCTTGCAG CCT....... .......... .......... 121 TTCTATTTCT ACATGAAACT TATTTAGTTA TCTAACTAAT ACAATGAAAC AGATTGCTAC 81048 |||||||| .......... .......... .......... .......... .......... ..ATTGCTAC 129 AGCTCTATTT GCAAATTCAC CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG 80988 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTCTATTT GCAAATTCAC CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG 189 AAGGTATGTG TTTAAAAAAT ATAATGCTTG TTTCTTTGAG GACTATAATC GAAGTTGTAC 80928 ||| AAG....... .......... .......... .......... .......... .......... 192 ATAACAATTT TTTGTTTGTT TCACCAGCCA CATTTGGACC GATACAGATA ATAACCGCGC 80868 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......CCA CATTTGGACC GATACAGATA ATAACCGCGC 225 TGGGATGCTT CCTTTCGTCT TTGATGACTC TTTTGGGTAA GTTCTCCTGT TTTTGCAAAT 80808 |||||||||| |||||||||| |||||||||| |||||| TGGGATGCTT CCTTTCGTCT TTGATGACTC TTTTGG.... .......... .......... 261 AGTTATAGTT TCTTGAGTCG TGACATATTA TTTTCTTTCT TCAGTCAAGA TTACGTTTGT 80748 .......... .......... .......... .......... .......... .......... 261 TAAAGTTGGA GGGCAAATGG TAGGGGCAGT CGGAGTCATA TTAACATTTT CTTTTTCTTG 80688 .......... .......... .......... .......... .......... .......... 261 TGGTATTTGT AAATGAATTC TTTAAAACTA CTCATATGTC TAACCTGATT GTTTTGAAAA 80628 .......... .......... .......... .......... .......... .......... 261 AGTGAAGAAA CAATTCGACC TATTTACTTG AAACGGAAAG GGTAATTCAA CTTATACTAT 80568 .......... .......... .......... .......... .......... .......... 261 TTAACCACAT ATGTTGTGCT GGTTCATAAT TATATAGTAC ATGTGACCAC ATGAAGGAGA 80508 .......... .......... .......... .......... .......... .......... 261 CATTATTGTC TACTAGCAAT CAATTTCACT TCTTTTGAAT GCTGAGTTGA GGTTCATTTC 80448 .......... .......... .......... .......... .......... .......... 261 GGGCATTTAT GCCTTCACAC TATGATTTTC AGCTGGAATT GTGTATATAT CTTTTTGTTA 80388 .......... .......... .......... .......... .......... .......... 261 CCTAAGGCTG CTGTGGGATG GTCTTCAAAG CTTCTTGACT TGTCAGAAAA AACCGTGCTT 80328 .......... .......... .......... .......... .......... .......... 261 CCTTGTACTT TTAAGCGCAT GGTCCTTTTG TTTAATGTAT CTCATGGTGA ATGCACTTGT 80268 .......... .......... .......... .......... .......... .......... 261 CGTACATTAT ACACGAGCTA GGTTAGGGAA ATGGATAAGA TTTGTTGGAG TTCGAGATGG 80208 .......... .......... .......... .......... .......... .......... 261 GCTGTCCTAA CCTGTTTTGA TGTTTGTCAA CAGAGCTATG TGATGGGTTA GGAATCAAAT 80148 .......... .......... .......... .......... .......... .......... 261 GAAATTGTGT ATTTAGTGTT AGCAAAATTC ATCTTTTGTT GTCATCTTCA GAACTTCCAT 80088 .......... .......... .......... .......... .......... .......... 261 TGATTAATTT CGTTTTCACA GCAAACATTT TCTTTTCTAA TGGACGCAGG TTTGAGCAAT 80028 | |||||||||| .......... .......... .......... .......... .........G TTTGAGCAAT 272 ATGTAGATTA TGCACTTGAT GTCCCAATGT ATTTTGTCTA TCGGAAGAAG AAGTATGTTG 79968 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTAGATTA TGCACTTGAT GTCCCAATGT ATTTTGTCTA TCGGAAGAAG AAGTATGTTG 332 ATTGTACTGG ATTGTCTTTC CGGGTATGTT ATTTATAACA ATAACAGATT TATAAATGTC 79908 |||||||||| |||||||||| ||| ATTGTACTGG ATTGTCTTTC CGG....... .......... .......... .......... 355 ACTCTCTTGT GCTGATTTAA CAACATTGCT GCAGGACTTC ATGAATGGAA AACTTCCGCC 79848 |||||| |||||||||| |||||||||| .......... .......... .......... ....GACTTC ATGAATGGAA AACTTCCGCC 381 CATTCCCGGC GAATACCCTA CTCTTAATGA TTGGGAGAAT CATCTCACAA CAATATTTCC 79788 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTCCCGGC GAATACCCTA CTCTTAATGA TTGGGAGAAT CATCTCACAA CAATATTTCC 441 TGAGGTATGC AAGCTTCATC CAAGAAAATG TCCTTTACTA CTGGTTTGTC TATGTATGGT 79728 |||| TGAG...... .......... .......... .......... .......... .......... 445 TATGGAAGTA TGAACTCGGG TTAACTATAT CAGGTCAGAC TCAAAAGATA TCTGGAAATG 79668 ||||||| |||||||||| |||||||||| .......... .......... .......... ...GTCAGAC TCAAAAGATA TCTGGAAATG 472 AGAGGTGCTG ATGGAGGGCC TTGGAGACGG TTATGCGCAT TGCCTGCATT CTGGGTATGC 79608 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| AGAGGTGCTG ATGGAGGGCC TTGGAGACGG TTATGCGCAT TGCCTGCATT CTGG...... 526 AATCTGACTC TAGGATTTAT GATGTTGTTT TTTGTAAAAG AAGGAAACAA AAAGCGAGTA 79548 .......... .......... .......... .......... .......... .......... 526 TTACTCGGTA AAGCACCAGG GAAAGGGAGA ATAGAATATT GTGCTCACCT CACCTCCCAC 79488 .......... .......... .......... .......... .......... .......... 526 TGAGAGGAAC TTCTTACTCT TTCTCCTGTT TGTTGGGACA ATTGCAAAGT TTGTTGTGGC 79428 .......... .......... .......... .......... .......... .......... 526 TACTTTGAAC CAAACAGCTC AGTCATCTTC TTTTTTTCCT TTTCTTTTTT TCAGGTGGGT 79368 |||||| .......... .......... .......... .......... .......... ....GTGGGT 532 ATACTCTACG ATGAGGGGTC TTTGCAAAGC GTTTTGGACA TGACATTTGA TTGGACTGCA 79308 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATACTCTACG ATGAGGGGTC TTTGCAAAGC GTTTTGGACA TGACATTTGA TTGGACTGCA 592 GAAGAAAGAG ACATGTTAAG GAAT 79284 |||||||||| |||||||||| |||| GAAGAAAGAG ACATGTTAAG GAAT 616 hqPGS_C08HBa0251H04.1-_SGN-E250603+ (81467 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79614,79373 79284) ******************************************************************************** EST sequence 116 +strand 454 n (File: SGN-E269213+) 1 GCAAATTCAC CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG AAGGCACATT 61 TGGACGCGAT ACAGATAATA ACCGCGCTGG GATGCTTCCT TTCGTCTTTG ATGACTCTTT 121 TGGGTTTGAG CAATATGTAG ATTATGCACT TGATGTCCCA ATGTATTTTG TCTATCGGAA 181 GAAGAAGTAT GTTGATTGTA CTGGATTGTC TTTCCGGGAC TTCATGAATG GAAAACTTCC 241 GCCCATTCCC GGCGAATACC CTACTCTTAA TGATTGGGAG AATCATCTCA CAACAATATT 301 TCCTGAGGTC AGACTCAAAA GATATCTGGA AATGAGAGGT GCTGATGGAG GGCCTTGGAG 361 ACGGTTATGC GCATTGCCTG CATTCTGGGT ATGCAATCTG ACTCTAGGAT TTATGATGTT 421 GTTTTTTGTA AAAGAAGGAA ACAAAAAGCG AGTA Predicted gene structure (within gDNA segment 81637 to 78938): Exon 1 81037 80985 ( 53 n); cDNA 1 53 ( 53 n); score: 1.000 Intron 1 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 0.93) Exon 2 80900 80832 ( 69 n); cDNA 54 123 ( 70 n); score: 0.949 Intron 2 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 3 80038 79945 ( 94 n); cDNA 124 217 ( 94 n); score: 1.000 Intron 3 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 4 79873 79784 ( 90 n); cDNA 218 307 ( 90 n); score: 1.000 Intron 4 79783 79695 ( 89 n); Pd: 0.999 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 5 79694 79548 ( 147 n); cDNA 308 454 ( 147 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E269213+ 0.992 453 0.998 C PGS_C08HBa0251H04.1-_SGN-E269213+ (81037 80985,80900 80832,80038 79945,79873 79784,79694 79548) Alignment (genomic DNA sequence = upper lines): GCAAATTCAC CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG AAGGTATGTG 80978 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| GCAAATTCAC CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG AAG....... 53 TTTAAAAAAT ATAATGCTTG TTTCTTTGAG GACTATAATC GAAGTTGTAC ATAACAATTT 80918 .......... .......... .......... .......... .......... .......... 53 TTTGTTTGTT TCACCAGCCA CATTTGGAC- CGATACAGAT AATAACCGCG CTGGGATGCT 80859 || ||||||||| |||||||||| |||||||||| |||||||||| .......... .......GCA CATTTGGACG CGATACAGAT AATAACCGCG CTGGGATGCT 96 TCCTTTCGTC TTTGATGACT CTTTTGGGTA AGTTCTCCTG TTTTTGCAAA TAGTTATAGT 80799 |||||||||| |||||||||| ||||||| TCCTTTCGTC TTTGATGACT CTTTTGG... .......... .......... .......... 123 TTCTTGAGTC GTGACATATT ATTTTCTTTC TTCAGTCAAG ATTACGTTTG TTAAAGTTGG 80739 .......... .......... .......... .......... .......... .......... 123 AGGGCAAATG GTAGGGGCAG TCGGAGTCAT ATTAACATTT TCTTTTTCTT GTGGTATTTG 80679 .......... .......... .......... .......... .......... .......... 123 TAAATGAATT CTTTAAAACT ACTCATATGT CTAACCTGAT TGTTTTGAAA AAGTGAAGAA 80619 .......... .......... .......... .......... .......... .......... 123 ACAATTCGAC CTATTTACTT GAAACGGAAA GGGTAATTCA ACTTATACTA TTTAACCACA 80559 .......... .......... .......... .......... .......... .......... 123 TATGTTGTGC TGGTTCATAA TTATATAGTA CATGTGACCA CATGAAGGAG ACATTATTGT 80499 .......... .......... .......... .......... .......... .......... 123 CTACTAGCAA TCAATTTCAC TTCTTTTGAA TGCTGAGTTG AGGTTCATTT CGGGCATTTA 80439 .......... .......... .......... .......... .......... .......... 123 TGCCTTCACA CTATGATTTT CAGCTGGAAT TGTGTATATA TCTTTTTGTT ACCTAAGGCT 80379 .......... .......... .......... .......... .......... .......... 123 GCTGTGGGAT GGTCTTCAAA GCTTCTTGAC TTGTCAGAAA AAACCGTGCT TCCTTGTACT 80319 .......... .......... .......... .......... .......... .......... 123 TTTAAGCGCA TGGTCCTTTT GTTTAATGTA TCTCATGGTG AATGCACTTG TCGTACATTA 80259 .......... .......... .......... .......... .......... .......... 123 TACACGAGCT AGGTTAGGGA AATGGATAAG ATTTGTTGGA GTTCGAGATG GGCTGTCCTA 80199 .......... .......... .......... .......... .......... .......... 123 ACCTGTTTTG ATGTTTGTCA ACAGAGCTAT GTGATGGGTT AGGAATCAAA TGAAATTGTG 80139 .......... .......... .......... .......... .......... .......... 123 TATTTAGTGT TAGCAAAATT CATCTTTTGT TGTCATCTTC AGAACTTCCA TTGATTAATT 80079 .......... .......... .......... .......... .......... .......... 123 TCGTTTTCAC AGCAAACATT TTCTTTTCTA ATGGACGCAG GTTTGAGCAA TATGTAGATT 80019 |||||||||| |||||||||| .......... .......... .......... .......... GTTTGAGCAA TATGTAGATT 143 ATGCACTTGA TGTCCCAATG TATTTTGTCT ATCGGAAGAA GAAGTATGTT GATTGTACTG 79959 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGCACTTGA TGTCCCAATG TATTTTGTCT ATCGGAAGAA GAAGTATGTT GATTGTACTG 203 GATTGTCTTT CCGGGTATGT TATTTATAAC AATAACAGAT TTATAAATGT CACTCTCTTG 79899 |||||||||| |||| GATTGTCTTT CCGG...... .......... .......... .......... .......... 217 TGCTGATTTA ACAACATTGC TGCAGGACTT CATGAATGGA AAACTTCCGC CCATTCCCGG 79839 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....GACTT CATGAATGGA AAACTTCCGC CCATTCCCGG 252 CGAATACCCT ACTCTTAATG ATTGGGAGAA TCATCTCACA ACAATATTTC CTGAGGTATG 79779 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| CGAATACCCT ACTCTTAATG ATTGGGAGAA TCATCTCACA ACAATATTTC CTGAG..... 307 CAAGCTTCAT CCAAGAAAAT GTCCTTTACT ACTGGTTTGT CTATGTATGG TTATGGAAGT 79719 .......... .......... .......... .......... .......... .......... 307 ATGAACTCGG GTTAACTATA TCAGGTCAGA CTCAAAAGAT ATCTGGAAAT GAGAGGTGCT 79659 |||||| |||||||||| |||||||||| |||||||||| .......... .......... ....GTCAGA CTCAAAAGAT ATCTGGAAAT GAGAGGTGCT 343 GATGGAGGGC CTTGGAGACG GTTATGCGCA TTGCCTGCAT TCTGGGTATG CAATCTGACT 79599 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATGGAGGGC CTTGGAGACG GTTATGCGCA TTGCCTGCAT TCTGGGTATG CAATCTGACT 403 CTAGGATTTA TGATGTTGTT TTTTGTAAAA GAAGGAAACA AAAAGCGAGT A 79548 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | CTAGGATTTA TGATGTTGTT TTTTGTAAAA GAAGGAAACA AAAAGCGAGT A 454 hqPGS_C08HBa0251H04.1-_SGN-E269213+ (81037 80985,80900 80832,80038 79945,79873 79784,79694 79548) ******************************************************************************** EST sequence 75 +strand 732 n (File: SGN-E263535+) 1 TAGAACCTGG TGGTCAGTTT GAGCTTAGTG GTGCACCACT TGAAACACTG CATCAAACTT 61 GTGCAGAGGT TAATTCACAT CTTTACCAGG TTAAAGCTGT TGCAGAAGAG ATGGGAATTG 121 GATTCTTAGG AACTGGATTC CAGCCAAAGT GGGGGCTGAA AGATATACCA ATAATGCCGA 181 AGGGGAGATA TGAGATTATG AGAAATTACA TGCCTAAAGT TGGCTCACTT GGGCTGGATA 241 TGATGTTTAG AACATGCACT GTTCAGGTAA ATCTGGACTT CAGTTCTGAA GCTGACATGA 301 TCAGAAAGTT CCGTGCTGGT CTTGCCTTGC AGCCTATTGC TACAGCTCTA TTTGCAAATT 361 CACCTTTCAC TGAAGGAAAA CCTAATGGTT ATCTCAGCAA GAGAAGCCAC ATTTGGACCG 421 ATACAGATAA TAACCGCGCT GGGATGCTTC CTTTCGTCTT TGATGACTCT TTTGGGTTTG 481 AGCAATATGT AGATTATGCA CTTGATGTCC CAATGTATTT TGTCTATCGG AAGAAGAAGT 541 ATGTTGATTG TACTGGATTG TCTTTCCGGG ACTTCATGAA TGGAAAACTT CCGCCCATTC 601 CCGGCGAATA CCCTACTCTT AATGATTGGG AGAATCATCT CACAACAATA TTTCCTGAGG 661 TCAGACTCAA AAGATATCTG GAAAATGAGA GGTGCTGATG GAGGGCCTTG GAGACGGTTA 721 TGCGCATTGC CT Predicted gene structure (within gDNA segment 83909 to 79013): Exon 1 83309 83221 ( 89 n); cDNA 1 89 ( 89 n); score: 1.000 Intron 1 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 82773 82681 ( 93 n); cDNA 90 182 ( 93 n); score: 1.000 Intron 2 82680 81500 (1181 n); Pd: 0.977 (s: 1.00), Pa: 0.707 (s: 1.00) Exon 3 81499 81416 ( 84 n); cDNA 183 266 ( 84 n); score: 1.000 Intron 3 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 4 81203 81135 ( 69 n); cDNA 267 335 ( 69 n); score: 1.000 Intron 4 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 5 81055 80985 ( 71 n); cDNA 336 406 ( 71 n); score: 1.000 Intron 5 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 6 80900 80832 ( 69 n); cDNA 407 475 ( 69 n); score: 1.000 Intron 6 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 7 80038 79945 ( 94 n); cDNA 476 569 ( 94 n); score: 1.000 Intron 7 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 8 79873 79784 ( 90 n); cDNA 570 659 ( 90 n); score: 1.000 Intron 8 79783 79695 ( 89 n); Pd: 0.999 (s: 1.00), Pa: 0.991 (s: 0.95) Exon 9 79694 79623 ( 72 n); cDNA 660 732 ( 73 n); score: 0.965 MATCH C08HBa0251H04.1- SGN-E263535+ 0.997 731 0.999 C PGS_C08HBa0251H04.1-_SGN-E263535+ (83309 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79623) Alignment (genomic DNA sequence = upper lines): TAGAACCTGG TGGTCAGTTT GAGCTTAGTG GTGCACCACT TGAAACACTG CATCAAACTT 83250 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGAACCTGG TGGTCAGTTT GAGCTTAGTG GTGCACCACT TGAAACACTG CATCAAACTT 60 GTGCAGAGGT TAATTCACAT CTTTACCAGG TTTGTACTTT TGAAACTCCA TCAGTTTCCC 83190 |||||||||| |||||||||| ||||||||| GTGCAGAGGT TAATTCACAT CTTTACCAG. .......... .......... .......... 89 TTTTAATGAA ATAGTGGGCA ATTAAGATCT TTCTAACTAT ACATAGTCTG CACTGGGGAA 83130 .......... .......... .......... .......... .......... .......... 89 ATGTAAGCGA ACTTTAATTA TGCGATAACA TGGCTTGGAA TAAAAGGGAA TCTCATTTTT 83070 .......... .......... .......... .......... .......... .......... 89 TTGAGTTTGA GAAATCCTGC AAATGTGTCG GCCATTTTTG CTTCAGGTGG TTGATGACTT 83010 .......... .......... .......... .......... .......... .......... 89 ATTACACTAG GGATTCTTTA CCCAGTATTC TGGTTCTTCT GATCAGAGTC TCTAGTTGTA 82950 .......... .......... .......... .......... .......... .......... 89 TAAGAAAATG TTGCTTCAAA AGAACATTGT TGGGGTGAAA GTAAAGTTAT GACTTCCTGA 82890 .......... .......... .......... .......... .......... .......... 89 CTGTCTTGTC GATGAACATT TGGAGGCAAG AAAACCGAAG CTATTCTCAC AGGAGAAATA 82830 .......... .......... .......... .......... .......... .......... 89 TTCACGGATA AATTTAAGTT TTGCTGATAT GTAATAGTTT TCGTCTAATG GTACAGGTTA 82770 |||| .......... .......... .......... .......... .......... ......GTTA 93 AAGCTGTTGC AGAAGAGATG GGAATTGGAT TCTTAGGAAC TGGATTCCAG CCAAAGTGGG 82710 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCTGTTGC AGAAGAGATG GGAATTGGAT TCTTAGGAAC TGGATTCCAG CCAAAGTGGG 153 GGCTGAAAGA TATACCAATA ATGCCGAAGG TAAAAACAAT ATGCTGATCG ATGTTGTAAC 82650 |||||||||| |||||||||| ||||||||| GGCTGAAAGA TATACCAATA ATGCCGAAG. .......... .......... .......... 182 CAAAAGATTT GTTTGGTGTG TTGGTAACTT AGCATCTTAT CTCTTTGAGA CTGCTATTTT 82590 .......... .......... .......... .......... .......... .......... 182 AACAGTGAAA AGATGTTAAT ATTTTTGTAC ACGACCATTT CACATATTGT TCAGCATGTC 82530 .......... .......... .......... .......... .......... .......... 182 AAGTGCAGTA GAATCCCAGT TTCTAATATA ATTTCATTAT TCATTATTAT TTGATGAGAC 82470 .......... .......... .......... .......... .......... .......... 182 TAGTGTAGCT CGAAGCTCAT TCTCTCTGAT TTCATCATTT TGCAACACCT CATCTTTTTA 82410 .......... .......... .......... .......... .......... .......... 182 CCTACAAACC TTACCAGACA ATGCCTATTT CGCATAGTTA TTTTTGAAGT GAAATTTTTT 82350 .......... .......... .......... .......... .......... .......... 182 TCTAATCCAC CTTCGATCAT GAAAATCATG AATAAGTTAA TCATCGTTGA TATTTCTAGG 82290 .......... .......... .......... .......... .......... .......... 182 AGCCGAAGAG ATGACAGATG AGATATTGTT CTAATGATTA GTTATTCAGA TAAATGGTTC 82230 .......... .......... .......... .......... .......... .......... 182 AGATGTAGCT TGTCTCACAT CTTATTTCTT CTCTAAAAGA CACAAGAACA AGTAAATAAA 82170 .......... .......... .......... .......... .......... .......... 182 GGTGTAGAAC TTCAATTCTT TCCTTTTGAG CTAATCATTC ATCTTCTCCA TTAGGCTAAT 82110 .......... .......... .......... .......... .......... .......... 182 CTAGATTAAC AATCCCCACT TCTTCGACTC TTCTCTTACG GATTTAATAA AAAGGACGAA 82050 .......... .......... .......... .......... .......... .......... 182 GGAGAAAGAA CAAAAGAAAG CTAAAAAAAT CTTTTAGTTG CTGTACTATT CTCTTACTGT 81990 .......... .......... .......... .......... .......... .......... 182 CAACTCGAAG ATTAAAAAAT TTAGCTTTCT TTTTCTTTTC CTGATCAAAA AGAAAATTAC 81930 .......... .......... .......... .......... .......... .......... 182 GATTTGACAG TAAGGGAATG CTAAACCTGA TCCTTGGACC AGATCTAGTA ATTATTTCCA 81870 .......... .......... .......... .......... .......... .......... 182 ATAAATGGAA ATTATATATA GATCTTGCCT ACCTTTTCCC CCCAGAGTCT TCACAATTTA 81810 .......... .......... .......... .......... .......... .......... 182 GAAATGCAAA TAGTTTCCCC CCACTTGTTT CTTTTATGTT CTTTACTTAT TTACTTTTAA 81750 .......... .......... .......... .......... .......... .......... 182 CTAAGCCAGA ATTATGGTAT GTTATTTTGA TTTGCAGCTG TAATTTAAAA ATAATATTTG 81690 .......... .......... .......... .......... .......... .......... 182 CACCATCTAC CTCTATTCAT ATTCTGTACT ATGTACAACA TCATGTGCTT CTCAGACCTC 81630 .......... .......... .......... .......... .......... .......... 182 AATATTATTT GTGTTGTCAT GTTCTTCTAG CTTTGAAAGA TATATCTATT TCTTACTTTT 81570 .......... .......... .......... .......... .......... .......... 182 CTCAAGTATG ATTTGACCTC TTAGATCTGC GATCAGCTTG TGTTTTCACT GACAAAGTAC 81510 .......... .......... .......... .......... .......... .......... 182 TTTGACTCAG GGGAGATATG AGATTATGAG AAATTACATG CCTAAAGTTG GCTCACTTGG 81450 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... GGGAGATATG AGATTATGAG AAATTACATG CCTAAAGTTG GCTCACTTGG 232 GCTGGATATG ATGTTTAGAA CATGCACTGT TCAGGTAAGC TGAATGGTTT AGCTTTTTTC 81390 |||||||||| |||||||||| |||||||||| |||| GCTGGATATG ATGTTTAGAA CATGCACTGT TCAG...... .......... .......... 266 TTTTCATTTC GTTCCATTTT TTTGATGGGA TGCCATTGCC CCTTGACTAT TGGAGTGCAT 81330 .......... .......... .......... .......... .......... .......... 266 ACCCCTGTGC AGCATTCAAA GCTATATTTT TTTTTCCTGC CCATTTGTGT CTGGGTTCCC 81270 .......... .......... .......... .......... .......... .......... 266 GTCATGCCCT GCATGGTAAC ATTCATTAGT AAGTTGAATC AATATTTACG AATATGTTGT 81210 .......... .......... .......... .......... .......... .......... 266 TGACAGGTAA ATCTGGACTT CAGTTCTGAA GCTGACATGA TCAGAAAGTT CCGTGCTGGT 81150 |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ......GTAA ATCTGGACTT CAGTTCTGAA GCTGACATGA TCAGAAAGTT CCGTGCTGGT 320 CTTGCCTTGC AGCCTGTATG GTACCTTTAT CCTTTCTGTC CTTTCTATTT CTACATGAAA 81090 |||||||||| ||||| CTTGCCTTGC AGCCT..... .......... .......... .......... .......... 335 CTTATTTAGT TATCTAACTA ATACAATGAA ACAGATTGCT ACAGCTCTAT TTGCAAATTC 81030 |||||| |||||||||| |||||||||| .......... .......... .......... ....ATTGCT ACAGCTCTAT TTGCAAATTC 361 ACCTTTCACT GAAGGAAAAC CTAATGGTTA TCTCAGCAAG AGAAGGTATG TGTTTAAAAA 80970 |||||||||| |||||||||| |||||||||| |||||||||| ||||| ACCTTTCACT GAAGGAAAAC CTAATGGTTA TCTCAGCAAG AGAAG..... .......... 406 ATATAATGCT TGTTTCTTTG AGGACTATAA TCGAAGTTGT ACATAACAAT TTTTTGTTTG 80910 .......... .......... .......... .......... .......... .......... 406 TTTCACCAGC CACATTTGGA CCGATACAGA TAATAACCGC GCTGGGATGC TTCCTTTCGT 80850 | |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .........C CACATTTGGA CCGATACAGA TAATAACCGC GCTGGGATGC TTCCTTTCGT 457 CTTTGATGAC TCTTTTGGGT AAGTTCTCCT GTTTTTGCAA ATAGTTATAG TTTCTTGAGT 80790 |||||||||| |||||||| CTTTGATGAC TCTTTTGG.. .......... .......... .......... .......... 475 CGTGACATAT TATTTTCTTT CTTCAGTCAA GATTACGTTT GTTAAAGTTG GAGGGCAAAT 80730 .......... .......... .......... .......... .......... .......... 475 GGTAGGGGCA GTCGGAGTCA TATTAACATT TTCTTTTTCT TGTGGTATTT GTAAATGAAT 80670 .......... .......... .......... .......... .......... .......... 475 TCTTTAAAAC TACTCATATG TCTAACCTGA TTGTTTTGAA AAAGTGAAGA AACAATTCGA 80610 .......... .......... .......... .......... .......... .......... 475 CCTATTTACT TGAAACGGAA AGGGTAATTC AACTTATACT ATTTAACCAC ATATGTTGTG 80550 .......... .......... .......... .......... .......... .......... 475 CTGGTTCATA ATTATATAGT ACATGTGACC ACATGAAGGA GACATTATTG TCTACTAGCA 80490 .......... .......... .......... .......... .......... .......... 475 ATCAATTTCA CTTCTTTTGA ATGCTGAGTT GAGGTTCATT TCGGGCATTT ATGCCTTCAC 80430 .......... .......... .......... .......... .......... .......... 475 ACTATGATTT TCAGCTGGAA TTGTGTATAT ATCTTTTTGT TACCTAAGGC TGCTGTGGGA 80370 .......... .......... .......... .......... .......... .......... 475 TGGTCTTCAA AGCTTCTTGA CTTGTCAGAA AAAACCGTGC TTCCTTGTAC TTTTAAGCGC 80310 .......... .......... .......... .......... .......... .......... 475 ATGGTCCTTT TGTTTAATGT ATCTCATGGT GAATGCACTT GTCGTACATT ATACACGAGC 80250 .......... .......... .......... .......... .......... .......... 475 TAGGTTAGGG AAATGGATAA GATTTGTTGG AGTTCGAGAT GGGCTGTCCT AACCTGTTTT 80190 .......... .......... .......... .......... .......... .......... 475 GATGTTTGTC AACAGAGCTA TGTGATGGGT TAGGAATCAA ATGAAATTGT GTATTTAGTG 80130 .......... .......... .......... .......... .......... .......... 475 TTAGCAAAAT TCATCTTTTG TTGTCATCTT CAGAACTTCC ATTGATTAAT TTCGTTTTCA 80070 .......... .......... .......... .......... .......... .......... 475 CAGCAAACAT TTTCTTTTCT AATGGACGCA GGTTTGAGCA ATATGTAGAT TATGCACTTG 80010 ||||||||| |||||||||| |||||||||| .......... .......... .......... .GTTTGAGCA ATATGTAGAT TATGCACTTG 504 ATGTCCCAAT GTATTTTGTC TATCGGAAGA AGAAGTATGT TGATTGTACT GGATTGTCTT 79950 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTCCCAAT GTATTTTGTC TATCGGAAGA AGAAGTATGT TGATTGTACT GGATTGTCTT 564 TCCGGGTATG TTATTTATAA CAATAACAGA TTTATAAATG TCACTCTCTT GTGCTGATTT 79890 ||||| TCCGG..... .......... .......... .......... .......... .......... 569 AACAACATTG CTGCAGGACT TCATGAATGG AAAACTTCCG CCCATTCCCG GCGAATACCC 79830 |||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ......GACT TCATGAATGG AAAACTTCCG CCCATTCCCG GCGAATACCC 613 TACTCTTAAT GATTGGGAGA ATCATCTCAC AACAATATTT CCTGAGGTAT GCAAGCTTCA 79770 |||||||||| |||||||||| |||||||||| |||||||||| |||||| TACTCTTAAT GATTGGGAGA ATCATCTCAC AACAATATTT CCTGAG.... .......... 659 TCCAAGAAAA TGTCCTTTAC TACTGGTTTG TCTATGTATG GTTATGGAAG TATGAACTCG 79710 .......... .......... .......... .......... .......... .......... 659 GGTTAACTAT ATCAGGTCAG ACTCAAAAGA TATCTGG-AA ATGAGAGGTG CTGATGGAGG 79651 ||||| |||||||||| ||||||| || |||||||||| |||||||||| .......... .....GTCAG ACTCAAAAGA TATCTGGAAA ATGAGAGGTG CTGATGGAGG 704 GCCTTGGAGA CGGTTATGCG CATTGCCT 79623 |||||||||| |||||||||| |||||||| GCCTTGGAGA CGGTTATGCG CATTGCCT 732 hqPGS_C08HBa0251H04.1-_SGN-E263535+ (83309 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79623) ******************************************************************************** EST sequence 89 +strand 638 n (File: SGN-E212583+) 1 GCAGAGGTTA ATTCACATCT TTACCAGGTT AAAGCTGTTG CAGAAGAGAT GGGAATTGGA 61 TTCTTAGGAA CTGGATTCCA GCCAAAGTGG GGGCTGAAAG ATATACCAAT AATGCCGAAG 121 GGGAGATATG AGATTATGAG AAATTACATG CCTAAAGTTG GCTCACTTGG GCTGGATATG 181 ATGTTTAGAA CATGCACTGT TCAGGTAAAT CTGGACTTCA GTTCTGAAGC TGACATGATC 241 AGAAAGTTCC GTGCTGGTCT TGCCTTGCAG CCTATTGCTA CAGCTCTATT TGCAAATTCA 301 CCTTTCACTG AAGGAAAACC TAATGGTTAT CTCAGCAAGA GAAGCCACAT TTGGACCGAT 361 ACAGATAATA ACCGCGCTGG GATGCTTCCT TTCGTCTTTG ATGACTCTTT TGGGTTTGAG 421 CAATATGTAG ATTATGCACT TGATGTCCCA ATGTATTTTG TCTATCGGAA GAAGAAGTAT 481 GTTGATTGTA CTGGATTGTC TTTCCGGGAC TTCATGAATG GAAAACTTCC GCCCATTCCC 541 GGCGAATACC CTACTCTTAA TGATTGGGAG AATCATCTCA CAACAATATT TCCTGAGGTC 601 AGACTCAAAA GATATCTGGA AAATGAGAGT GCTGATGG Predicted gene structure (within gDNA segment 83847 to 78782): Exon 1 83247 83221 ( 27 n); cDNA 1 27 ( 27 n); score: 1.000 Intron 1 83220 82774 ( 447 n); Pd: 0.987 (s: 0), Pa: 0.999 (s: 1.00) Exon 2 82773 82681 ( 93 n); cDNA 28 120 ( 93 n); score: 1.000 Intron 2 82680 81500 (1181 n); Pd: 0.977 (s: 1.00), Pa: 0.707 (s: 1.00) Exon 3 81499 81416 ( 84 n); cDNA 121 204 ( 84 n); score: 1.000 Intron 3 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 4 81203 81135 ( 69 n); cDNA 205 273 ( 69 n); score: 1.000 Intron 4 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 5 81055 80985 ( 71 n); cDNA 274 344 ( 71 n); score: 1.000 Intron 5 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 6 80900 80832 ( 69 n); cDNA 345 413 ( 69 n); score: 1.000 Intron 6 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 7 80038 79945 ( 94 n); cDNA 414 507 ( 94 n); score: 1.000 Intron 7 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 8 79873 79784 ( 90 n); cDNA 508 597 ( 90 n); score: 1.000 Intron 8 79783 79695 ( 89 n); Pd: 0.999 (s: 1.00), Pa: 0.991 (s: 0.91) Exon 9 79694 79654 ( 41 n); cDNA 598 638 ( 41 n); score: 0.915 MATCH C08HBa0251H04.1- SGN-E212583+ 1.000 638 1.000 C PGS_C08HBa0251H04.1-_SGN-E212583+ (83247 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79654) Alignment (genomic DNA sequence = upper lines): GCAGAGGTTA ATTCACATCT TTACCAGGTT TGTACTTTTG AAACTCCATC AGTTTCCCTT 83188 |||||||||| |||||||||| ||||||| GCAGAGGTTA ATTCACATCT TTACCAG... .......... .......... .......... 27 TTAATGAAAT AGTGGGCAAT TAAGATCTTT CTAACTATAC ATAGTCTGCA CTGGGGAAAT 83128 .......... .......... .......... .......... .......... .......... 27 GTAAGCGAAC TTTAATTATG CGATAACATG GCTTGGAATA AAAGGGAATC TCATTTTTTT 83068 .......... .......... .......... .......... .......... .......... 27 GAGTTTGAGA AATCCTGCAA ATGTGTCGGC CATTTTTGCT TCAGGTGGTT GATGACTTAT 83008 .......... .......... .......... .......... .......... .......... 27 TACACTAGGG ATTCTTTACC CAGTATTCTG GTTCTTCTGA TCAGAGTCTC TAGTTGTATA 82948 .......... .......... .......... .......... .......... .......... 27 AGAAAATGTT GCTTCAAAAG AACATTGTTG GGGTGAAAGT AAAGTTATGA CTTCCTGACT 82888 .......... .......... .......... .......... .......... .......... 27 GTCTTGTCGA TGAACATTTG GAGGCAAGAA AACCGAAGCT ATTCTCACAG GAGAAATATT 82828 .......... .......... .......... .......... .......... .......... 27 CACGGATAAA TTTAAGTTTT GCTGATATGT AATAGTTTTC GTCTAATGGT ACAGGTTAAA 82768 |||||| .......... .......... .......... .......... .......... ....GTTAAA 33 GCTGTTGCAG AAGAGATGGG AATTGGATTC TTAGGAACTG GATTCCAGCC AAAGTGGGGG 82708 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTGTTGCAG AAGAGATGGG AATTGGATTC TTAGGAACTG GATTCCAGCC AAAGTGGGGG 93 CTGAAAGATA TACCAATAAT GCCGAAGGTA AAAACAATAT GCTGATCGAT GTTGTAACCA 82648 |||||||||| |||||||||| ||||||| CTGAAAGATA TACCAATAAT GCCGAAG... .......... .......... .......... 120 AAAGATTTGT TTGGTGTGTT GGTAACTTAG CATCTTATCT CTTTGAGACT GCTATTTTAA 82588 .......... .......... .......... .......... .......... .......... 120 CAGTGAAAAG ATGTTAATAT TTTTGTACAC GACCATTTCA CATATTGTTC AGCATGTCAA 82528 .......... .......... .......... .......... .......... .......... 120 GTGCAGTAGA ATCCCAGTTT CTAATATAAT TTCATTATTC ATTATTATTT GATGAGACTA 82468 .......... .......... .......... .......... .......... .......... 120 GTGTAGCTCG AAGCTCATTC TCTCTGATTT CATCATTTTG CAACACCTCA TCTTTTTACC 82408 .......... .......... .......... .......... .......... .......... 120 TACAAACCTT ACCAGACAAT GCCTATTTCG CATAGTTATT TTTGAAGTGA AATTTTTTTC 82348 .......... .......... .......... .......... .......... .......... 120 TAATCCACCT TCGATCATGA AAATCATGAA TAAGTTAATC ATCGTTGATA TTTCTAGGAG 82288 .......... .......... .......... .......... .......... .......... 120 CCGAAGAGAT GACAGATGAG ATATTGTTCT AATGATTAGT TATTCAGATA AATGGTTCAG 82228 .......... .......... .......... .......... .......... .......... 120 ATGTAGCTTG TCTCACATCT TATTTCTTCT CTAAAAGACA CAAGAACAAG TAAATAAAGG 82168 .......... .......... .......... .......... .......... .......... 120 TGTAGAACTT CAATTCTTTC CTTTTGAGCT AATCATTCAT CTTCTCCATT AGGCTAATCT 82108 .......... .......... .......... .......... .......... .......... 120 AGATTAACAA TCCCCACTTC TTCGACTCTT CTCTTACGGA TTTAATAAAA AGGACGAAGG 82048 .......... .......... .......... .......... .......... .......... 120 AGAAAGAACA AAAGAAAGCT AAAAAAATCT TTTAGTTGCT GTACTATTCT CTTACTGTCA 81988 .......... .......... .......... .......... .......... .......... 120 ACTCGAAGAT TAAAAAATTT AGCTTTCTTT TTCTTTTCCT GATCAAAAAG AAAATTACGA 81928 .......... .......... .......... .......... .......... .......... 120 TTTGACAGTA AGGGAATGCT AAACCTGATC CTTGGACCAG ATCTAGTAAT TATTTCCAAT 81868 .......... .......... .......... .......... .......... .......... 120 AAATGGAAAT TATATATAGA TCTTGCCTAC CTTTTCCCCC CAGAGTCTTC ACAATTTAGA 81808 .......... .......... .......... .......... .......... .......... 120 AATGCAAATA GTTTCCCCCC ACTTGTTTCT TTTATGTTCT TTACTTATTT ACTTTTAACT 81748 .......... .......... .......... .......... .......... .......... 120 AAGCCAGAAT TATGGTATGT TATTTTGATT TGCAGCTGTA ATTTAAAAAT AATATTTGCA 81688 .......... .......... .......... .......... .......... .......... 120 CCATCTACCT CTATTCATAT TCTGTACTAT GTACAACATC ATGTGCTTCT CAGACCTCAA 81628 .......... .......... .......... .......... .......... .......... 120 TATTATTTGT GTTGTCATGT TCTTCTAGCT TTGAAAGATA TATCTATTTC TTACTTTTCT 81568 .......... .......... .......... .......... .......... .......... 120 CAAGTATGAT TTGACCTCTT AGATCTGCGA TCAGCTTGTG TTTTCACTGA CAAAGTACTT 81508 .......... .......... .......... .......... .......... .......... 120 TGACTCAGGG GAGATATGAG ATTATGAGAA ATTACATGCC TAAAGTTGGC TCACTTGGGC 81448 || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ........GG GAGATATGAG ATTATGAGAA ATTACATGCC TAAAGTTGGC TCACTTGGGC 172 TGGATATGAT GTTTAGAACA TGCACTGTTC AGGTAAGCTG AATGGTTTAG CTTTTTTCTT 81388 |||||||||| |||||||||| |||||||||| || TGGATATGAT GTTTAGAACA TGCACTGTTC AG........ .......... .......... 204 TTCATTTCGT TCCATTTTTT TGATGGGATG CCATTGCCCC TTGACTATTG GAGTGCATAC 81328 .......... .......... .......... .......... .......... .......... 204 CCCTGTGCAG CATTCAAAGC TATATTTTTT TTTCCTGCCC ATTTGTGTCT GGGTTCCCGT 81268 .......... .......... .......... .......... .......... .......... 204 CATGCCCTGC ATGGTAACAT TCATTAGTAA GTTGAATCAA TATTTACGAA TATGTTGTTG 81208 .......... .......... .......... .......... .......... .......... 204 ACAGGTAAAT CTGGACTTCA GTTCTGAAGC TGACATGATC AGAAAGTTCC GTGCTGGTCT 81148 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....GTAAAT CTGGACTTCA GTTCTGAAGC TGACATGATC AGAAAGTTCC GTGCTGGTCT 260 TGCCTTGCAG CCTGTATGGT ACCTTTATCC TTTCTGTCCT TTCTATTTCT ACATGAAACT 81088 |||||||||| ||| TGCCTTGCAG CCT....... .......... .......... .......... .......... 273 TATTTAGTTA TCTAACTAAT ACAATGAAAC AGATTGCTAC AGCTCTATTT GCAAATTCAC 81028 |||||||| |||||||||| |||||||||| .......... .......... .......... ..ATTGCTAC AGCTCTATTT GCAAATTCAC 301 CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG AAGGTATGTG TTTAAAAAAT 80968 |||||||||| |||||||||| |||||||||| |||||||||| ||| CTTTCACTGA AGGAAAACCT AATGGTTATC TCAGCAAGAG AAG....... .......... 344 ATAATGCTTG TTTCTTTGAG GACTATAATC GAAGTTGTAC ATAACAATTT TTTGTTTGTT 80908 .......... .......... .......... .......... .......... .......... 344 TCACCAGCCA CATTTGGACC GATACAGATA ATAACCGCGC TGGGATGCTT CCTTTCGTCT 80848 ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......CCA CATTTGGACC GATACAGATA ATAACCGCGC TGGGATGCTT CCTTTCGTCT 397 TTGATGACTC TTTTGGGTAA GTTCTCCTGT TTTTGCAAAT AGTTATAGTT TCTTGAGTCG 80788 |||||||||| |||||| TTGATGACTC TTTTGG.... .......... .......... .......... .......... 413 TGACATATTA TTTTCTTTCT TCAGTCAAGA TTACGTTTGT TAAAGTTGGA GGGCAAATGG 80728 .......... .......... .......... .......... .......... .......... 413 TAGGGGCAGT CGGAGTCATA TTAACATTTT CTTTTTCTTG TGGTATTTGT AAATGAATTC 80668 .......... .......... .......... .......... .......... .......... 413 TTTAAAACTA CTCATATGTC TAACCTGATT GTTTTGAAAA AGTGAAGAAA CAATTCGACC 80608 .......... .......... .......... .......... .......... .......... 413 TATTTACTTG AAACGGAAAG GGTAATTCAA CTTATACTAT TTAACCACAT ATGTTGTGCT 80548 .......... .......... .......... .......... .......... .......... 413 GGTTCATAAT TATATAGTAC ATGTGACCAC ATGAAGGAGA CATTATTGTC TACTAGCAAT 80488 .......... .......... .......... .......... .......... .......... 413 CAATTTCACT TCTTTTGAAT GCTGAGTTGA GGTTCATTTC GGGCATTTAT GCCTTCACAC 80428 .......... .......... .......... .......... .......... .......... 413 TATGATTTTC AGCTGGAATT GTGTATATAT CTTTTTGTTA CCTAAGGCTG CTGTGGGATG 80368 .......... .......... .......... .......... .......... .......... 413 GTCTTCAAAG CTTCTTGACT TGTCAGAAAA AACCGTGCTT CCTTGTACTT TTAAGCGCAT 80308 .......... .......... .......... .......... .......... .......... 413 GGTCCTTTTG TTTAATGTAT CTCATGGTGA ATGCACTTGT CGTACATTAT ACACGAGCTA 80248 .......... .......... .......... .......... .......... .......... 413 GGTTAGGGAA ATGGATAAGA TTTGTTGGAG TTCGAGATGG GCTGTCCTAA CCTGTTTTGA 80188 .......... .......... .......... .......... .......... .......... 413 TGTTTGTCAA CAGAGCTATG TGATGGGTTA GGAATCAAAT GAAATTGTGT ATTTAGTGTT 80128 .......... .......... .......... .......... .......... .......... 413 AGCAAAATTC ATCTTTTGTT GTCATCTTCA GAACTTCCAT TGATTAATTT CGTTTTCACA 80068 .......... .......... .......... .......... .......... .......... 413 GCAAACATTT TCTTTTCTAA TGGACGCAGG TTTGAGCAAT ATGTAGATTA TGCACTTGAT 80008 | |||||||||| |||||||||| |||||||||| .......... .......... .........G TTTGAGCAAT ATGTAGATTA TGCACTTGAT 444 GTCCCAATGT ATTTTGTCTA TCGGAAGAAG AAGTATGTTG ATTGTACTGG ATTGTCTTTC 79948 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTCCCAATGT ATTTTGTCTA TCGGAAGAAG AAGTATGTTG ATTGTACTGG ATTGTCTTTC 504 CGGGTATGTT ATTTATAACA ATAACAGATT TATAAATGTC ACTCTCTTGT GCTGATTTAA 79888 ||| CGG....... .......... .......... .......... .......... .......... 507 CAACATTGCT GCAGGACTTC ATGAATGGAA AACTTCCGCC CATTCCCGGC GAATACCCTA 79828 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....GACTTC ATGAATGGAA AACTTCCGCC CATTCCCGGC GAATACCCTA 553 CTCTTAATGA TTGGGAGAAT CATCTCACAA CAATATTTCC TGAGGTATGC AAGCTTCATC 79768 |||||||||| |||||||||| |||||||||| |||||||||| |||| CTCTTAATGA TTGGGAGAAT CATCTCACAA CAATATTTCC TGAG...... .......... 597 CAAGAAAATG TCCTTTACTA CTGGTTTGTC TATGTATGGT TATGGAAGTA TGAACTCGGG 79708 .......... .......... .......... .......... .......... .......... 597 TTAACTATAT CAGGTCAGAC TCAAAAGATA TCTGG-AAAT GAGAGGTGCT GATGG 79654 ||||||| |||||||||| ||||| |||| |||| ||||| ||||| .......... ...GTCAGAC TCAAAAGATA TCTGGAAAAT GAGA-GTGCT GATGG 638 hqPGS_C08HBa0251H04.1-_SGN-E212583+ (83247 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79654) ******************************************************************************** EST sequence 65 +strand 771 n (File: SGN-E261343+) 1 GAAGTATGAT CAAATAGCTG ACTTGCTAAA TGGTATTGCT GAGCGGTTTG ATTGGGAAAA 61 AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAGGGA AAGCAAAGCA TATCATTAGA 121 ACCTGGTGGT CAGTTTGAGC TTAGTGGTGC ACCACTTGAA ACACTGCATC AAACTTGTGC 181 AGAGGTTAAT TCACATCTTT ACCAGGTTAA AGCTGTTGCA GAAGAGATGG GAATTGGATT 241 CTTAGGAACT GGATTCCAGC CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAGGG 301 GAGATATGAG ATTATGAGAA ATTACATGCC TAAAGTTGGC TCACTTGGGC TGGATATGAT 361 GTTTAGAACA TGCACTGTTC AGGTAAATCT GGACTTCAGT TCTGAAGCTG ACATGATCAG 421 AAAGTTCCGT GCTGGTCTTG CCTTGCAGCC TATTGCTACA GCTCTATTTG CAAATTCACC 481 TTTCACTGAA GGAAAACCTA ATGGTTATCT CAGCAAGAGA AGCCACATTT GGACCGATAC 541 AGATAATAAC CGCGCTGGGA TGCTTCCTTT CGTCTTTGAT GACTCTTTTG GGTTTGAGCA 601 ATATGTAGAT TATGCACTTG ATGTCCCAAT GTATTTTGTC TATCGGAAGA AGAAGTATGT 661 TGATTGTACT GGATTGTCTT TCCGGGACTT CATGAATGGA AAACTTCCGC CCATTCCCGG 721 CGAATACCCT ACTCTTAATG ATTGGGAGAA TCATCTCACA CAATATTTCC T Predicted gene structure (within gDNA segment 84218 to 79079): Exon 1 83618 83522 ( 97 n); cDNA 1 97 ( 97 n); score: 1.000 Intron 1 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 2 83328 83221 ( 108 n); cDNA 98 205 ( 108 n); score: 1.000 Intron 2 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 82773 82681 ( 93 n); cDNA 206 298 ( 93 n); score: 1.000 Intron 3 82680 81500 (1181 n); Pd: 0.977 (s: 1.00), Pa: 0.707 (s: 1.00) Exon 4 81499 81416 ( 84 n); cDNA 299 382 ( 84 n); score: 1.000 Intron 4 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 81203 81135 ( 69 n); cDNA 383 451 ( 69 n); score: 1.000 Intron 5 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 1.00) Exon 6 81055 80985 ( 71 n); cDNA 452 522 ( 71 n); score: 1.000 Intron 6 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 7 80900 80832 ( 69 n); cDNA 523 591 ( 69 n); score: 1.000 Intron 7 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 8 80038 79945 ( 94 n); cDNA 592 685 ( 94 n); score: 1.000 Intron 8 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 9 79873 79787 ( 87 n); cDNA 686 771 ( 86 n); score: 0.989 MATCH C08HBa0251H04.1- SGN-E261343+ 0.999 772 1.001 C PGS_C08HBa0251H04.1-_SGN-E261343+ (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79787) Alignment (genomic DNA sequence = upper lines): GAAGTATGAT CAAATAGCTG ACTTGCTAAA TGGTATTGCT GAGCGGTTTG ATTGGGAAAA 83559 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTATGAT CAAATAGCTG ACTTGCTAAA TGGTATTGCT GAGCGGTTTG ATTGGGAAAA 60 AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAGGTA ACGTGAACTT GGTGCAAACT 83499 |||||||||| |||||||||| |||||||||| ||||||| AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAG... .......... .......... 97 TTGGTTTAAA ATATTCTAGG TTGAATTTGT CATGGCTCCA TACAATGTAT TATGCTACTT 83439 .......... .......... .......... .......... .......... .......... 97 ACCATGAATA GAATCATAAT TCCTGGTTCA TTTTTTTCCT CAAGTGCAAG TCTGTAGTAA 83379 .......... .......... .......... .......... .......... .......... 97 GTAGTAATTA CTTTAGCATA ATTGGTGGAT AATTTGCACT GTCTTCGCAG GGAAAGCAAA 83319 |||||||||| .......... .......... .......... .......... .......... GGAAAGCAAA 107 GCATATCATT AGAACCTGGT GGTCAGTTTG AGCTTAGTGG TGCACCACTT GAAACACTGC 83259 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATATCATT AGAACCTGGT GGTCAGTTTG AGCTTAGTGG TGCACCACTT GAAACACTGC 167 ATCAAACTTG TGCAGAGGTT AATTCACATC TTTACCAGGT TTGTACTTTT GAAACTCCAT 83199 |||||||||| |||||||||| |||||||||| |||||||| ATCAAACTTG TGCAGAGGTT AATTCACATC TTTACCAG.. .......... .......... 205 CAGTTTCCCT TTTAATGAAA TAGTGGGCAA TTAAGATCTT TCTAACTATA CATAGTCTGC 83139 .......... .......... .......... .......... .......... .......... 205 ACTGGGGAAA TGTAAGCGAA CTTTAATTAT GCGATAACAT GGCTTGGAAT AAAAGGGAAT 83079 .......... .......... .......... .......... .......... .......... 205 CTCATTTTTT TGAGTTTGAG AAATCCTGCA AATGTGTCGG CCATTTTTGC TTCAGGTGGT 83019 .......... .......... .......... .......... .......... .......... 205 TGATGACTTA TTACACTAGG GATTCTTTAC CCAGTATTCT GGTTCTTCTG ATCAGAGTCT 82959 .......... .......... .......... .......... .......... .......... 205 CTAGTTGTAT AAGAAAATGT TGCTTCAAAA GAACATTGTT GGGGTGAAAG TAAAGTTATG 82899 .......... .......... .......... .......... .......... .......... 205 ACTTCCTGAC TGTCTTGTCG ATGAACATTT GGAGGCAAGA AAACCGAAGC TATTCTCACA 82839 .......... .......... .......... .......... .......... .......... 205 GGAGAAATAT TCACGGATAA ATTTAAGTTT TGCTGATATG TAATAGTTTT CGTCTAATGG 82779 .......... .......... .......... .......... .......... .......... 205 TACAGGTTAA AGCTGTTGCA GAAGAGATGG GAATTGGATT CTTAGGAACT GGATTCCAGC 82719 ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .....GTTAA AGCTGTTGCA GAAGAGATGG GAATTGGATT CTTAGGAACT GGATTCCAGC 260 CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAGGT AAAAACAATA TGCTGATCGA 82659 |||||||||| |||||||||| |||||||||| |||||||| CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAG.. .......... .......... 298 TGTTGTAACC AAAAGATTTG TTTGGTGTGT TGGTAACTTA GCATCTTATC TCTTTGAGAC 82599 .......... .......... .......... .......... .......... .......... 298 TGCTATTTTA ACAGTGAAAA GATGTTAATA TTTTTGTACA CGACCATTTC ACATATTGTT 82539 .......... .......... .......... .......... .......... .......... 298 CAGCATGTCA AGTGCAGTAG AATCCCAGTT TCTAATATAA TTTCATTATT CATTATTATT 82479 .......... .......... .......... .......... .......... .......... 298 TGATGAGACT AGTGTAGCTC GAAGCTCATT CTCTCTGATT TCATCATTTT GCAACACCTC 82419 .......... .......... .......... .......... .......... .......... 298 ATCTTTTTAC CTACAAACCT TACCAGACAA TGCCTATTTC GCATAGTTAT TTTTGAAGTG 82359 .......... .......... .......... .......... .......... .......... 298 AAATTTTTTT CTAATCCACC TTCGATCATG AAAATCATGA ATAAGTTAAT CATCGTTGAT 82299 .......... .......... .......... .......... .......... .......... 298 ATTTCTAGGA GCCGAAGAGA TGACAGATGA GATATTGTTC TAATGATTAG TTATTCAGAT 82239 .......... .......... .......... .......... .......... .......... 298 AAATGGTTCA GATGTAGCTT GTCTCACATC TTATTTCTTC TCTAAAAGAC ACAAGAACAA 82179 .......... .......... .......... .......... .......... .......... 298 GTAAATAAAG GTGTAGAACT TCAATTCTTT CCTTTTGAGC TAATCATTCA TCTTCTCCAT 82119 .......... .......... .......... .......... .......... .......... 298 TAGGCTAATC TAGATTAACA ATCCCCACTT CTTCGACTCT TCTCTTACGG ATTTAATAAA 82059 .......... .......... .......... .......... .......... .......... 298 AAGGACGAAG GAGAAAGAAC AAAAGAAAGC TAAAAAAATC TTTTAGTTGC TGTACTATTC 81999 .......... .......... .......... .......... .......... .......... 298 TCTTACTGTC AACTCGAAGA TTAAAAAATT TAGCTTTCTT TTTCTTTTCC TGATCAAAAA 81939 .......... .......... .......... .......... .......... .......... 298 GAAAATTACG ATTTGACAGT AAGGGAATGC TAAACCTGAT CCTTGGACCA GATCTAGTAA 81879 .......... .......... .......... .......... .......... .......... 298 TTATTTCCAA TAAATGGAAA TTATATATAG ATCTTGCCTA CCTTTTCCCC CCAGAGTCTT 81819 .......... .......... .......... .......... .......... .......... 298 CACAATTTAG AAATGCAAAT AGTTTCCCCC CACTTGTTTC TTTTATGTTC TTTACTTATT 81759 .......... .......... .......... .......... .......... .......... 298 TACTTTTAAC TAAGCCAGAA TTATGGTATG TTATTTTGAT TTGCAGCTGT AATTTAAAAA 81699 .......... .......... .......... .......... .......... .......... 298 TAATATTTGC ACCATCTACC TCTATTCATA TTCTGTACTA TGTACAACAT CATGTGCTTC 81639 .......... .......... .......... .......... .......... .......... 298 TCAGACCTCA ATATTATTTG TGTTGTCATG TTCTTCTAGC TTTGAAAGAT ATATCTATTT 81579 .......... .......... .......... .......... .......... .......... 298 CTTACTTTTC TCAAGTATGA TTTGACCTCT TAGATCTGCG ATCAGCTTGT GTTTTCACTG 81519 .......... .......... .......... .......... .......... .......... 298 ACAAAGTACT TTGACTCAGG GGAGATATGA GATTATGAGA AATTACATGC CTAAAGTTGG 81459 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........G GGAGATATGA GATTATGAGA AATTACATGC CTAAAGTTGG 339 CTCACTTGGG CTGGATATGA TGTTTAGAAC ATGCACTGTT CAGGTAAGCT GAATGGTTTA 81399 |||||||||| |||||||||| |||||||||| |||||||||| ||| CTCACTTGGG CTGGATATGA TGTTTAGAAC ATGCACTGTT CAG....... .......... 382 GCTTTTTTCT TTTCATTTCG TTCCATTTTT TTGATGGGAT GCCATTGCCC CTTGACTATT 81339 .......... .......... .......... .......... .......... .......... 382 GGAGTGCATA CCCCTGTGCA GCATTCAAAG CTATATTTTT TTTTCCTGCC CATTTGTGTC 81279 .......... .......... .......... .......... .......... .......... 382 TGGGTTCCCG TCATGCCCTG CATGGTAACA TTCATTAGTA AGTTGAATCA ATATTTACGA 81219 .......... .......... .......... .......... .......... .......... 382 ATATGTTGTT GACAGGTAAA TCTGGACTTC AGTTCTGAAG CTGACATGAT CAGAAAGTTC 81159 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GTAAA TCTGGACTTC AGTTCTGAAG CTGACATGAT CAGAAAGTTC 427 CGTGCTGGTC TTGCCTTGCA GCCTGTATGG TACCTTTATC CTTTCTGTCC TTTCTATTTC 81099 |||||||||| |||||||||| |||| CGTGCTGGTC TTGCCTTGCA GCCT...... .......... .......... .......... 451 TACATGAAAC TTATTTAGTT ATCTAACTAA TACAATGAAA CAGATTGCTA CAGCTCTATT 81039 ||||||| |||||||||| .......... .......... .......... .......... ...ATTGCTA CAGCTCTATT 468 TGCAAATTCA CCTTTCACTG AAGGAAAACC TAATGGTTAT CTCAGCAAGA GAAGGTATGT 80979 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| TGCAAATTCA CCTTTCACTG AAGGAAAACC TAATGGTTAT CTCAGCAAGA GAAG...... 522 GTTTAAAAAA TATAATGCTT GTTTCTTTGA GGACTATAAT CGAAGTTGTA CATAACAATT 80919 .......... .......... .......... .......... .......... .......... 522 TTTTGTTTGT TTCACCAGCC ACATTTGGAC CGATACAGAT AATAACCGCG CTGGGATGCT 80859 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........CC ACATTTGGAC CGATACAGAT AATAACCGCG CTGGGATGCT 564 TCCTTTCGTC TTTGATGACT CTTTTGGGTA AGTTCTCCTG TTTTTGCAAA TAGTTATAGT 80799 |||||||||| |||||||||| ||||||| TCCTTTCGTC TTTGATGACT CTTTTGG... .......... .......... .......... 591 TTCTTGAGTC GTGACATATT ATTTTCTTTC TTCAGTCAAG ATTACGTTTG TTAAAGTTGG 80739 .......... .......... .......... .......... .......... .......... 591 AGGGCAAATG GTAGGGGCAG TCGGAGTCAT ATTAACATTT TCTTTTTCTT GTGGTATTTG 80679 .......... .......... .......... .......... .......... .......... 591 TAAATGAATT CTTTAAAACT ACTCATATGT CTAACCTGAT TGTTTTGAAA AAGTGAAGAA 80619 .......... .......... .......... .......... .......... .......... 591 ACAATTCGAC CTATTTACTT GAAACGGAAA GGGTAATTCA ACTTATACTA TTTAACCACA 80559 .......... .......... .......... .......... .......... .......... 591 TATGTTGTGC TGGTTCATAA TTATATAGTA CATGTGACCA CATGAAGGAG ACATTATTGT 80499 .......... .......... .......... .......... .......... .......... 591 CTACTAGCAA TCAATTTCAC TTCTTTTGAA TGCTGAGTTG AGGTTCATTT CGGGCATTTA 80439 .......... .......... .......... .......... .......... .......... 591 TGCCTTCACA CTATGATTTT CAGCTGGAAT TGTGTATATA TCTTTTTGTT ACCTAAGGCT 80379 .......... .......... .......... .......... .......... .......... 591 GCTGTGGGAT GGTCTTCAAA GCTTCTTGAC TTGTCAGAAA AAACCGTGCT TCCTTGTACT 80319 .......... .......... .......... .......... .......... .......... 591 TTTAAGCGCA TGGTCCTTTT GTTTAATGTA TCTCATGGTG AATGCACTTG TCGTACATTA 80259 .......... .......... .......... .......... .......... .......... 591 TACACGAGCT AGGTTAGGGA AATGGATAAG ATTTGTTGGA GTTCGAGATG GGCTGTCCTA 80199 .......... .......... .......... .......... .......... .......... 591 ACCTGTTTTG ATGTTTGTCA ACAGAGCTAT GTGATGGGTT AGGAATCAAA TGAAATTGTG 80139 .......... .......... .......... .......... .......... .......... 591 TATTTAGTGT TAGCAAAATT CATCTTTTGT TGTCATCTTC AGAACTTCCA TTGATTAATT 80079 .......... .......... .......... .......... .......... .......... 591 TCGTTTTCAC AGCAAACATT TTCTTTTCTA ATGGACGCAG GTTTGAGCAA TATGTAGATT 80019 |||||||||| |||||||||| .......... .......... .......... .......... GTTTGAGCAA TATGTAGATT 611 ATGCACTTGA TGTCCCAATG TATTTTGTCT ATCGGAAGAA GAAGTATGTT GATTGTACTG 79959 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGCACTTGA TGTCCCAATG TATTTTGTCT ATCGGAAGAA GAAGTATGTT GATTGTACTG 671 GATTGTCTTT CCGGGTATGT TATTTATAAC AATAACAGAT TTATAAATGT CACTCTCTTG 79899 |||||||||| |||| GATTGTCTTT CCGG...... .......... .......... .......... .......... 685 TGCTGATTTA ACAACATTGC TGCAGGACTT CATGAATGGA AAACTTCCGC CCATTCCCGG 79839 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....GACTT CATGAATGGA AAACTTCCGC CCATTCCCGG 720 CGAATACCCT ACTCTTAATG ATTGGGAGAA TCATCTCACA ACAATATTTC CT 79787 |||||||||| |||||||||| |||||||||| ||||||||| |||||||||| || CGAATACCCT ACTCTTAATG ATTGGGAGAA TCATCTCAC- ACAATATTTC CT 771 hqPGS_C08HBa0251H04.1-_SGN-E261343+ (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79787) ******************************************************************************** EST sequence 69 +strand 515 n (File: SGN-E250238+) 1 TGGATTCTTA GGAACTGGAT TCCAGCCAAA GTGGGGGCTG AAAGATAGAC CAATGGTGCC 61 GAAGGGGAGA TATGAGATTA TGAGAAATTA CATGCCTAAA GTTGGCTCAC TTGGGCTGGA 121 TATGATGTTT AGAACATGCA CTGTTCAGGT AAATCTGGAC TTCAGTTCTG AAGCTGACAT 181 GATCAGAAAG TTCCGTGCTG GTCTTGCCTT GCAGCCTATT GCTANNGGGC TATTTGCAAA 241 TTCACCTTTC ACTGAAGGAA AACCTAATGG TTATCTCAGC AAGAGAAGCC ACATTTGGAC 301 CGATACAGAT AATAACCGCG CTGGGATGCT TCCTTTCGTC TTTGATGACT CTTTTGGGTT 361 TGAGCAATAT GTAGATTATG CACTTGATGT CCCAATGTAT TTTGTCTATC GGAAGAAGAA 421 GTATGTTGAT TGTACTGGAT TGTCTTTCCG GGACTTCATG AATGGAAAAC TTCCGCCCAT 481 TTCCCGGGGA ATACCCTACT CTTAATGATT GGGAG Predicted gene structure (within gDNA segment 83344 to 79201): Exon 1 82744 82681 ( 64 n); cDNA 1 64 ( 64 n); score: 0.953 Intron 1 82680 81500 (1181 n); Pd: 0.977 (s: 0.94), Pa: 0.707 (s: 1.00) Exon 2 81499 81416 ( 84 n); cDNA 65 148 ( 84 n); score: 1.000 Intron 2 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 81203 81135 ( 69 n); cDNA 149 217 ( 69 n); score: 1.000 Intron 3 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 0.92) Exon 4 81055 80985 ( 71 n); cDNA 218 288 ( 71 n); score: 0.944 Intron 4 80984 80901 ( 84 n); Pd: 0.998 (s: 1.00), Pa: 0.996 (s: 1.00) Exon 5 80900 80832 ( 69 n); cDNA 289 357 ( 69 n); score: 1.000 Intron 5 80831 80039 ( 793 n); Pd: 1.000 (s: 1.00), Pa: 0.982 (s: 1.00) Exon 6 80038 79945 ( 94 n); cDNA 358 451 ( 94 n); score: 1.000 Intron 6 79944 79874 ( 71 n); Pd: 0.939 (s: 1.00), Pa: 0.993 (s: 0.93) Exon 7 79873 79811 ( 63 n); cDNA 452 515 ( 64 n); score: 0.944 MATCH C08HBa0251H04.1- SGN-E250238+ 0.980 514 0.998 C PGS_C08HBa0251H04.1-_SGN-E250238+ (82744 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79811) Alignment (genomic DNA sequence = upper lines): TGGATTCTTA GGAACTGGAT TCCAGCCAAA GTGGGGGCTG AAAGATATAC CAATAATGCC 82685 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| || |||| |||| TGGATTCTTA GGAACTGGAT TCCAGCCAAA GTGGGGGCTG AAAGATAGAC CAATGGTGCC 60 GAAGGTAAAA ACAATATGCT GATCGATGTT GTAACCAAAA GATTTGTTTG GTGTGTTGGT 82625 |||| GAAG...... .......... .......... .......... .......... .......... 64 AACTTAGCAT CTTATCTCTT TGAGACTGCT ATTTTAACAG TGAAAAGATG TTAATATTTT 82565 .......... .......... .......... .......... .......... .......... 64 TGTACACGAC CATTTCACAT ATTGTTCAGC ATGTCAAGTG CAGTAGAATC CCAGTTTCTA 82505 .......... .......... .......... .......... .......... .......... 64 ATATAATTTC ATTATTCATT ATTATTTGAT GAGACTAGTG TAGCTCGAAG CTCATTCTCT 82445 .......... .......... .......... .......... .......... .......... 64 CTGATTTCAT CATTTTGCAA CACCTCATCT TTTTACCTAC AAACCTTACC AGACAATGCC 82385 .......... .......... .......... .......... .......... .......... 64 TATTTCGCAT AGTTATTTTT GAAGTGAAAT TTTTTTCTAA TCCACCTTCG ATCATGAAAA 82325 .......... .......... .......... .......... .......... .......... 64 TCATGAATAA GTTAATCATC GTTGATATTT CTAGGAGCCG AAGAGATGAC AGATGAGATA 82265 .......... .......... .......... .......... .......... .......... 64 TTGTTCTAAT GATTAGTTAT TCAGATAAAT GGTTCAGATG TAGCTTGTCT CACATCTTAT 82205 .......... .......... .......... .......... .......... .......... 64 TTCTTCTCTA AAAGACACAA GAACAAGTAA ATAAAGGTGT AGAACTTCAA TTCTTTCCTT 82145 .......... .......... .......... .......... .......... .......... 64 TTGAGCTAAT CATTCATCTT CTCCATTAGG CTAATCTAGA TTAACAATCC CCACTTCTTC 82085 .......... .......... .......... .......... .......... .......... 64 GACTCTTCTC TTACGGATTT AATAAAAAGG ACGAAGGAGA AAGAACAAAA GAAAGCTAAA 82025 .......... .......... .......... .......... .......... .......... 64 AAAATCTTTT AGTTGCTGTA CTATTCTCTT ACTGTCAACT CGAAGATTAA AAAATTTAGC 81965 .......... .......... .......... .......... .......... .......... 64 TTTCTTTTTC TTTTCCTGAT CAAAAAGAAA ATTACGATTT GACAGTAAGG GAATGCTAAA 81905 .......... .......... .......... .......... .......... .......... 64 CCTGATCCTT GGACCAGATC TAGTAATTAT TTCCAATAAA TGGAAATTAT ATATAGATCT 81845 .......... .......... .......... .......... .......... .......... 64 TGCCTACCTT TTCCCCCCAG AGTCTTCACA ATTTAGAAAT GCAAATAGTT TCCCCCCACT 81785 .......... .......... .......... .......... .......... .......... 64 TGTTTCTTTT ATGTTCTTTA CTTATTTACT TTTAACTAAG CCAGAATTAT GGTATGTTAT 81725 .......... .......... .......... .......... .......... .......... 64 TTTGATTTGC AGCTGTAATT TAAAAATAAT ATTTGCACCA TCTACCTCTA TTCATATTCT 81665 .......... .......... .......... .......... .......... .......... 64 GTACTATGTA CAACATCATG TGCTTCTCAG ACCTCAATAT TATTTGTGTT GTCATGTTCT 81605 .......... .......... .......... .......... .......... .......... 64 TCTAGCTTTG AAAGATATAT CTATTTCTTA CTTTTCTCAA GTATGATTTG ACCTCTTAGA 81545 .......... .......... .......... .......... .......... .......... 64 TCTGCGATCA GCTTGTGTTT TCACTGACAA AGTACTTTGA CTCAGGGGAG ATATGAGATT 81485 ||||| |||||||||| .......... .......... .......... .......... .....GGGAG ATATGAGATT 79 ATGAGAAATT ACATGCCTAA AGTTGGCTCA CTTGGGCTGG ATATGATGTT TAGAACATGC 81425 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAGAAATT ACATGCCTAA AGTTGGCTCA CTTGGGCTGG ATATGATGTT TAGAACATGC 139 ACTGTTCAGG TAAGCTGAAT GGTTTAGCTT TTTTCTTTTC ATTTCGTTCC ATTTTTTTGA 81365 ||||||||| ACTGTTCAG. .......... .......... .......... .......... .......... 148 TGGGATGCCA TTGCCCCTTG ACTATTGGAG TGCATACCCC TGTGCAGCAT TCAAAGCTAT 81305 .......... .......... .......... .......... .......... .......... 148 ATTTTTTTTT CCTGCCCATT TGTGTCTGGG TTCCCGTCAT GCCCTGCATG GTAACATTCA 81245 .......... .......... .......... .......... .......... .......... 148 TTAGTAAGTT GAATCAATAT TTACGAATAT GTTGTTGACA GGTAAATCTG GACTTCAGTT 81185 ||||||||| |||||||||| .......... .......... .......... .......... .GTAAATCTG GACTTCAGTT 167 CTGAAGCTGA CATGATCAGA AAGTTCCGTG CTGGTCTTGC CTTGCAGCCT GTATGGTACC 81125 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGAAGCTGA CATGATCAGA AAGTTCCGTG CTGGTCTTGC CTTGCAGCCT .......... 217 TTTATCCTTT CTGTCCTTTC TATTTCTACA TGAAACTTAT TTAGTTATCT AACTAATACA 81065 .......... .......... .......... .......... .......... .......... 217 ATGAAACAGA TTGCTACAGC TCTATTTGCA AATTCACCTT TCACTGAAGG AAAACCTAAT 81005 | |||||| | ||||||||| |||||||||| |||||||||| |||||||||| .........A TTGCTANNGG GCTATTTGCA AATTCACCTT TCACTGAAGG AAAACCTAAT 268 GGTTATCTCA GCAAGAGAAG GTATGTGTTT AAAAAATATA ATGCTTGTTT CTTTGAGGAC 80945 |||||||||| |||||||||| GGTTATCTCA GCAAGAGAAG .......... .......... .......... .......... 288 TATAATCGAA GTTGTACATA ACAATTTTTT GTTTGTTTCA CCAGCCACAT TTGGACCGAT 80885 |||||| |||||||||| .......... .......... .......... .......... ....CCACAT TTGGACCGAT 304 ACAGATAATA ACCGCGCTGG GATGCTTCCT TTCGTCTTTG ATGACTCTTT TGGGTAAGTT 80825 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| ACAGATAATA ACCGCGCTGG GATGCTTCCT TTCGTCTTTG ATGACTCTTT TGG....... 357 CTCCTGTTTT TGCAAATAGT TATAGTTTCT TGAGTCGTGA CATATTATTT TCTTTCTTCA 80765 .......... .......... .......... .......... .......... .......... 357 GTCAAGATTA CGTTTGTTAA AGTTGGAGGG CAAATGGTAG GGGCAGTCGG AGTCATATTA 80705 .......... .......... .......... .......... .......... .......... 357 ACATTTTCTT TTTCTTGTGG TATTTGTAAA TGAATTCTTT AAAACTACTC ATATGTCTAA 80645 .......... .......... .......... .......... .......... .......... 357 CCTGATTGTT TTGAAAAAGT GAAGAAACAA TTCGACCTAT TTACTTGAAA CGGAAAGGGT 80585 .......... .......... .......... .......... .......... .......... 357 AATTCAACTT ATACTATTTA ACCACATATG TTGTGCTGGT TCATAATTAT ATAGTACATG 80525 .......... .......... .......... .......... .......... .......... 357 TGACCACATG AAGGAGACAT TATTGTCTAC TAGCAATCAA TTTCACTTCT TTTGAATGCT 80465 .......... .......... .......... .......... .......... .......... 357 GAGTTGAGGT TCATTTCGGG CATTTATGCC TTCACACTAT GATTTTCAGC TGGAATTGTG 80405 .......... .......... .......... .......... .......... .......... 357 TATATATCTT TTTGTTACCT AAGGCTGCTG TGGGATGGTC TTCAAAGCTT CTTGACTTGT 80345 .......... .......... .......... .......... .......... .......... 357 CAGAAAAAAC CGTGCTTCCT TGTACTTTTA AGCGCATGGT CCTTTTGTTT AATGTATCTC 80285 .......... .......... .......... .......... .......... .......... 357 ATGGTGAATG CACTTGTCGT ACATTATACA CGAGCTAGGT TAGGGAAATG GATAAGATTT 80225 .......... .......... .......... .......... .......... .......... 357 GTTGGAGTTC GAGATGGGCT GTCCTAACCT GTTTTGATGT TTGTCAACAG AGCTATGTGA 80165 .......... .......... .......... .......... .......... .......... 357 TGGGTTAGGA ATCAAATGAA ATTGTGTATT TAGTGTTAGC AAAATTCATC TTTTGTTGTC 80105 .......... .......... .......... .......... .......... .......... 357 ATCTTCAGAA CTTCCATTGA TTAATTTCGT TTTCACAGCA AACATTTTCT TTTCTAATGG 80045 .......... .......... .......... .......... .......... .......... 357 ACGCAGGTTT GAGCAATATG TAGATTATGC ACTTGATGTC CCAATGTATT TTGTCTATCG 79985 |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ......GTTT GAGCAATATG TAGATTATGC ACTTGATGTC CCAATGTATT TTGTCTATCG 411 GAAGAAGAAG TATGTTGATT GTACTGGATT GTCTTTCCGG GTATGTTATT TATAACAATA 79925 |||||||||| |||||||||| |||||||||| |||||||||| GAAGAAGAAG TATGTTGATT GTACTGGATT GTCTTTCCGG .......... .......... 451 ACAGATTTAT AAATGTCACT CTCTTGTGCT GATTTAACAA CATTGCTGCA GGACTTCATG 79865 ||||||||| .......... .......... .......... .......... .......... .GACTTCATG 460 AATGGAAAAC TTCCGCCCA- TTCCCGGCGA ATACCCTACT CTTAATGATT GGGAG 79811 |||||||||| ||||||||| ||||||| || |||||||||| |||||||||| ||||| AATGGAAAAC TTCCGCCCAT TTCCCGGGGA ATACCCTACT CTTAATGATT GGGAG 515 hqPGS_C08HBa0251H04.1-_SGN-E250238+ (82744 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79811) ******************************************************************************** EST sequence 118 +strand 457 n (File: SGN-E256709+) 1 GAAGTATGAT CAAATTTCTG ACTTGCTAAA TGGTATTGCT GAACGGTGGG ATTGGGAAAA 61 AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAGGGA AAGCAAAGCA TATCATTAGA 121 ACCTGGTGGT CAGTTTGAGC TTAGTGGTGC ACCACTTGAA ACACTGCATC AAACTTGTGC 181 AGAGGTTAAT TCACATCTTT ACCAGGTTAA AGCTGTTGCA CAAGAGATGG GAATTGGATT 241 CTTAGGAACT GGATTCCAGC CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAGGG 301 GAGATATGAG ATTATGAGAA ATTACATGCC TAAAGTTGGC TCACTTGGGC TGGATATGAT 361 GTTTAGAACA TGCACTGTTC AGGTAAATCT GGACTTCAGT TCTGAAGCTG ACATGATCAG 421 AAAGTTCCGT GCTGGTCTTG CCTTGCAGCC TATTGCT Predicted gene structure (within gDNA segment 84371 to 80465): Exon 1 83618 83522 ( 97 n); cDNA 1 97 ( 97 n); score: 0.948 Intron 1 83521 83329 ( 193 n); Pd: 0.994 (s: 0.96), Pa: 0.994 (s: 1.00) Exon 2 83328 83221 ( 108 n); cDNA 98 205 ( 108 n); score: 1.000 Intron 2 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 0.98) Exon 3 82773 82681 ( 93 n); cDNA 206 298 ( 93 n); score: 0.989 Intron 3 82680 81500 (1181 n); Pd: 0.977 (s: 1.00), Pa: 0.707 (s: 1.00) Exon 4 81499 81416 ( 84 n); cDNA 299 382 ( 84 n); score: 1.000 Intron 4 81415 81204 ( 212 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 5 81203 81135 ( 69 n); cDNA 383 451 ( 69 n); score: 1.000 Intron 5 81134 81056 ( 79 n); Pd: 1.000 (s: 1.00), Pa: 0.986 (s: 0) Exon 6 81055 81050 ( 6 n); cDNA 452 457 ( 6 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E256709+ 0.987 457 1.000 C PGS_C08HBa0251H04.1-_SGN-E256709+ (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 81050) Alignment (genomic DNA sequence = upper lines): GAAGTATGAT CAAATAGCTG ACTTGCTAAA TGGTATTGCT GAGCGGTTTG ATTGGGAAAA 83559 |||||||||| ||||| ||| |||||||||| |||||||||| || |||| | |||||||||| GAAGTATGAT CAAATTTCTG ACTTGCTAAA TGGTATTGCT GAACGGTGGG ATTGGGAAAA 60 AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAGGTA ACGTGAACTT GGTGCAAACT 83499 |||||||||| |||||||||| |||||||||| ||||||| AGTAATGGAG GGTGACAAGA TTATTGGCCT GAAACAG... .......... .......... 97 TTGGTTTAAA ATATTCTAGG TTGAATTTGT CATGGCTCCA TACAATGTAT TATGCTACTT 83439 .......... .......... .......... .......... .......... .......... 97 ACCATGAATA GAATCATAAT TCCTGGTTCA TTTTTTTCCT CAAGTGCAAG TCTGTAGTAA 83379 .......... .......... .......... .......... .......... .......... 97 GTAGTAATTA CTTTAGCATA ATTGGTGGAT AATTTGCACT GTCTTCGCAG GGAAAGCAAA 83319 |||||||||| .......... .......... .......... .......... .......... GGAAAGCAAA 107 GCATATCATT AGAACCTGGT GGTCAGTTTG AGCTTAGTGG TGCACCACTT GAAACACTGC 83259 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATATCATT AGAACCTGGT GGTCAGTTTG AGCTTAGTGG TGCACCACTT GAAACACTGC 167 ATCAAACTTG TGCAGAGGTT AATTCACATC TTTACCAGGT TTGTACTTTT GAAACTCCAT 83199 |||||||||| |||||||||| |||||||||| |||||||| ATCAAACTTG TGCAGAGGTT AATTCACATC TTTACCAG.. .......... .......... 205 CAGTTTCCCT TTTAATGAAA TAGTGGGCAA TTAAGATCTT TCTAACTATA CATAGTCTGC 83139 .......... .......... .......... .......... .......... .......... 205 ACTGGGGAAA TGTAAGCGAA CTTTAATTAT GCGATAACAT GGCTTGGAAT AAAAGGGAAT 83079 .......... .......... .......... .......... .......... .......... 205 CTCATTTTTT TGAGTTTGAG AAATCCTGCA AATGTGTCGG CCATTTTTGC TTCAGGTGGT 83019 .......... .......... .......... .......... .......... .......... 205 TGATGACTTA TTACACTAGG GATTCTTTAC CCAGTATTCT GGTTCTTCTG ATCAGAGTCT 82959 .......... .......... .......... .......... .......... .......... 205 CTAGTTGTAT AAGAAAATGT TGCTTCAAAA GAACATTGTT GGGGTGAAAG TAAAGTTATG 82899 .......... .......... .......... .......... .......... .......... 205 ACTTCCTGAC TGTCTTGTCG ATGAACATTT GGAGGCAAGA AAACCGAAGC TATTCTCACA 82839 .......... .......... .......... .......... .......... .......... 205 GGAGAAATAT TCACGGATAA ATTTAAGTTT TGCTGATATG TAATAGTTTT CGTCTAATGG 82779 .......... .......... .......... .......... .......... .......... 205 TACAGGTTAA AGCTGTTGCA GAAGAGATGG GAATTGGATT CTTAGGAACT GGATTCCAGC 82719 ||||| |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| .....GTTAA AGCTGTTGCA CAAGAGATGG GAATTGGATT CTTAGGAACT GGATTCCAGC 260 CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAGGT AAAAACAATA TGCTGATCGA 82659 |||||||||| |||||||||| |||||||||| |||||||| CAAAGTGGGG GCTGAAAGAT ATACCAATAA TGCCGAAG.. .......... .......... 298 TGTTGTAACC AAAAGATTTG TTTGGTGTGT TGGTAACTTA GCATCTTATC TCTTTGAGAC 82599 .......... .......... .......... .......... .......... .......... 298 TGCTATTTTA ACAGTGAAAA GATGTTAATA TTTTTGTACA CGACCATTTC ACATATTGTT 82539 .......... .......... .......... .......... .......... .......... 298 CAGCATGTCA AGTGCAGTAG AATCCCAGTT TCTAATATAA TTTCATTATT CATTATTATT 82479 .......... .......... .......... .......... .......... .......... 298 TGATGAGACT AGTGTAGCTC GAAGCTCATT CTCTCTGATT TCATCATTTT GCAACACCTC 82419 .......... .......... .......... .......... .......... .......... 298 ATCTTTTTAC CTACAAACCT TACCAGACAA TGCCTATTTC GCATAGTTAT TTTTGAAGTG 82359 .......... .......... .......... .......... .......... .......... 298 AAATTTTTTT CTAATCCACC TTCGATCATG AAAATCATGA ATAAGTTAAT CATCGTTGAT 82299 .......... .......... .......... .......... .......... .......... 298 ATTTCTAGGA GCCGAAGAGA TGACAGATGA GATATTGTTC TAATGATTAG TTATTCAGAT 82239 .......... .......... .......... .......... .......... .......... 298 AAATGGTTCA GATGTAGCTT GTCTCACATC TTATTTCTTC TCTAAAAGAC ACAAGAACAA 82179 .......... .......... .......... .......... .......... .......... 298 GTAAATAAAG GTGTAGAACT TCAATTCTTT CCTTTTGAGC TAATCATTCA TCTTCTCCAT 82119 .......... .......... .......... .......... .......... .......... 298 TAGGCTAATC TAGATTAACA ATCCCCACTT CTTCGACTCT TCTCTTACGG ATTTAATAAA 82059 .......... .......... .......... .......... .......... .......... 298 AAGGACGAAG GAGAAAGAAC AAAAGAAAGC TAAAAAAATC TTTTAGTTGC TGTACTATTC 81999 .......... .......... .......... .......... .......... .......... 298 TCTTACTGTC AACTCGAAGA TTAAAAAATT TAGCTTTCTT TTTCTTTTCC TGATCAAAAA 81939 .......... .......... .......... .......... .......... .......... 298 GAAAATTACG ATTTGACAGT AAGGGAATGC TAAACCTGAT CCTTGGACCA GATCTAGTAA 81879 .......... .......... .......... .......... .......... .......... 298 TTATTTCCAA TAAATGGAAA TTATATATAG ATCTTGCCTA CCTTTTCCCC CCAGAGTCTT 81819 .......... .......... .......... .......... .......... .......... 298 CACAATTTAG AAATGCAAAT AGTTTCCCCC CACTTGTTTC TTTTATGTTC TTTACTTATT 81759 .......... .......... .......... .......... .......... .......... 298 TACTTTTAAC TAAGCCAGAA TTATGGTATG TTATTTTGAT TTGCAGCTGT AATTTAAAAA 81699 .......... .......... .......... .......... .......... .......... 298 TAATATTTGC ACCATCTACC TCTATTCATA TTCTGTACTA TGTACAACAT CATGTGCTTC 81639 .......... .......... .......... .......... .......... .......... 298 TCAGACCTCA ATATTATTTG TGTTGTCATG TTCTTCTAGC TTTGAAAGAT ATATCTATTT 81579 .......... .......... .......... .......... .......... .......... 298 CTTACTTTTC TCAAGTATGA TTTGACCTCT TAGATCTGCG ATCAGCTTGT GTTTTCACTG 81519 .......... .......... .......... .......... .......... .......... 298 ACAAAGTACT TTGACTCAGG GGAGATATGA GATTATGAGA AATTACATGC CTAAAGTTGG 81459 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........G GGAGATATGA GATTATGAGA AATTACATGC CTAAAGTTGG 339 CTCACTTGGG CTGGATATGA TGTTTAGAAC ATGCACTGTT CAGGTAAGCT GAATGGTTTA 81399 |||||||||| |||||||||| |||||||||| |||||||||| ||| CTCACTTGGG CTGGATATGA TGTTTAGAAC ATGCACTGTT CAG....... .......... 382 GCTTTTTTCT TTTCATTTCG TTCCATTTTT TTGATGGGAT GCCATTGCCC CTTGACTATT 81339 .......... .......... .......... .......... .......... .......... 382 GGAGTGCATA CCCCTGTGCA GCATTCAAAG CTATATTTTT TTTTCCTGCC CATTTGTGTC 81279 .......... .......... .......... .......... .......... .......... 382 TGGGTTCCCG TCATGCCCTG CATGGTAACA TTCATTAGTA AGTTGAATCA ATATTTACGA 81219 .......... .......... .......... .......... .......... .......... 382 ATATGTTGTT GACAGGTAAA TCTGGACTTC AGTTCTGAAG CTGACATGAT CAGAAAGTTC 81159 ||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .....GTAAA TCTGGACTTC AGTTCTGAAG CTGACATGAT CAGAAAGTTC 427 CGTGCTGGTC TTGCCTTGCA GCCTGTATGG TACCTTTATC CTTTCTGTCC TTTCTATTTC 81099 |||||||||| |||||||||| |||| CGTGCTGGTC TTGCCTTGCA GCCT...... .......... .......... .......... 451 TACATGAAAC TTATTTAGTT ATCTAACTAA TACAATGAAA CAGATTGCT 81050 |||||| .......... .......... .......... .......... ...ATTGCT 457 hqPGS_C08HBa0251H04.1-_SGN-E256709+ (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 81050) ******************************************************************************** EST sequence 126 +strand 360 n (File: SGN-E270608+) 1 ATGAAGTATG ATCAAATAGC TGACTTGCTA AATGGTATTG CTGAGCGGTT TGATTGGGAA 61 AAAGTAATGG AGGGTGACAA GATTATTGGC CTGAAACAGG GAAAGCAAAG CATATCATTA 121 GAACCTGGTG GTCAATTTGA GCTTAGTGGT GCACCACTTG AAACACTGCA TCAAACTTGT 181 GCAGAGGTTA ATTCACATCT TTACCAGGTT AAAGCTGTTG CAGAAAAGAT GGGAATTGGA 241 TTCTTAAGAA CTGGATTCCA GCCAAAGTGG GGGCTGAAAG ATATACCAAT AATGCCGAAG 301 GGGAGATATG AGATTATGAG AAATTACATG CCTAAAGTTG GCTCACTTGG GCTGGATATG Predicted gene structure (within gDNA segment 84220 to 80830): Exon 1 83620 83522 ( 99 n); cDNA 1 99 ( 99 n); score: 1.000 Intron 1 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 0.98) Exon 2 83328 83221 ( 108 n); cDNA 100 207 ( 108 n); score: 0.991 Intron 2 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 0.96) Exon 3 82773 82681 ( 93 n); cDNA 208 300 ( 93 n); score: 0.978 Intron 3 82680 81500 (1181 n); Pd: 0.977 (s: 1.00), Pa: 0.707 (s: 1.00) Exon 4 81499 81440 ( 60 n); cDNA 301 360 ( 60 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E270608+ 0.992 360 1.000 C PGS_C08HBa0251H04.1-_SGN-E270608+ (83620 83522,83328 83221,82773 82681,81499 81440) Alignment (genomic DNA sequence = upper lines): ATGAAGTATG ATCAAATAGC TGACTTGCTA AATGGTATTG CTGAGCGGTT TGATTGGGAA 83561 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGAAGTATG ATCAAATAGC TGACTTGCTA AATGGTATTG CTGAGCGGTT TGATTGGGAA 60 AAAGTAATGG AGGGTGACAA GATTATTGGC CTGAAACAGG TAACGTGAAC TTGGTGCAAA 83501 |||||||||| |||||||||| |||||||||| ||||||||| AAAGTAATGG AGGGTGACAA GATTATTGGC CTGAAACAG. .......... .......... 99 CTTTGGTTTA AAATATTCTA GGTTGAATTT GTCATGGCTC CATACAATGT ATTATGCTAC 83441 .......... .......... .......... .......... .......... .......... 99 TTACCATGAA TAGAATCATA ATTCCTGGTT CATTTTTTTC CTCAAGTGCA AGTCTGTAGT 83381 .......... .......... .......... .......... .......... .......... 99 AAGTAGTAAT TACTTTAGCA TAATTGGTGG ATAATTTGCA CTGTCTTCGC AGGGAAAGCA 83321 |||||||| .......... .......... .......... .......... .......... ..GGAAAGCA 107 AAGCATATCA TTAGAACCTG GTGGTCAGTT TGAGCTTAGT GGTGCACCAC TTGAAACACT 83261 |||||||||| |||||||||| ||||||| || |||||||||| |||||||||| |||||||||| AAGCATATCA TTAGAACCTG GTGGTCAATT TGAGCTTAGT GGTGCACCAC TTGAAACACT 167 GCATCAAACT TGTGCAGAGG TTAATTCACA TCTTTACCAG GTTTGTACTT TTGAAACTCC 83201 |||||||||| |||||||||| |||||||||| |||||||||| GCATCAAACT TGTGCAGAGG TTAATTCACA TCTTTACCAG .......... .......... 207 ATCAGTTTCC CTTTTAATGA AATAGTGGGC AATTAAGATC TTTCTAACTA TACATAGTCT 83141 .......... .......... .......... .......... .......... .......... 207 GCACTGGGGA AATGTAAGCG AACTTTAATT ATGCGATAAC ATGGCTTGGA ATAAAAGGGA 83081 .......... .......... .......... .......... .......... .......... 207 ATCTCATTTT TTTGAGTTTG AGAAATCCTG CAAATGTGTC GGCCATTTTT GCTTCAGGTG 83021 .......... .......... .......... .......... .......... .......... 207 GTTGATGACT TATTACACTA GGGATTCTTT ACCCAGTATT CTGGTTCTTC TGATCAGAGT 82961 .......... .......... .......... .......... .......... .......... 207 CTCTAGTTGT ATAAGAAAAT GTTGCTTCAA AAGAACATTG TTGGGGTGAA AGTAAAGTTA 82901 .......... .......... .......... .......... .......... .......... 207 TGACTTCCTG ACTGTCTTGT CGATGAACAT TTGGAGGCAA GAAAACCGAA GCTATTCTCA 82841 .......... .......... .......... .......... .......... .......... 207 CAGGAGAAAT ATTCACGGAT AAATTTAAGT TTTGCTGATA TGTAATAGTT TTCGTCTAAT 82781 .......... .......... .......... .......... .......... .......... 207 GGTACAGGTT AAAGCTGTTG CAGAAGAGAT GGGAATTGGA TTCTTAGGAA CTGGATTCCA 82721 ||| |||||||||| ||||| |||| |||||||||| |||||| ||| |||||||||| .......GTT AAAGCTGTTG CAGAAAAGAT GGGAATTGGA TTCTTAAGAA CTGGATTCCA 260 GCCAAAGTGG GGGCTGAAAG ATATACCAAT AATGCCGAAG GTAAAAACAA TATGCTGATC 82661 |||||||||| |||||||||| |||||||||| |||||||||| GCCAAAGTGG GGGCTGAAAG ATATACCAAT AATGCCGAAG .......... .......... 300 GATGTTGTAA CCAAAAGATT TGTTTGGTGT GTTGGTAACT TAGCATCTTA TCTCTTTGAG 82601 .......... .......... .......... .......... .......... .......... 300 ACTGCTATTT TAACAGTGAA AAGATGTTAA TATTTTTGTA CACGACCATT TCACATATTG 82541 .......... .......... .......... .......... .......... .......... 300 TTCAGCATGT CAAGTGCAGT AGAATCCCAG TTTCTAATAT AATTTCATTA TTCATTATTA 82481 .......... .......... .......... .......... .......... .......... 300 TTTGATGAGA CTAGTGTAGC TCGAAGCTCA TTCTCTCTGA TTTCATCATT TTGCAACACC 82421 .......... .......... .......... .......... .......... .......... 300 TCATCTTTTT ACCTACAAAC CTTACCAGAC AATGCCTATT TCGCATAGTT ATTTTTGAAG 82361 .......... .......... .......... .......... .......... .......... 300 TGAAATTTTT TTCTAATCCA CCTTCGATCA TGAAAATCAT GAATAAGTTA ATCATCGTTG 82301 .......... .......... .......... .......... .......... .......... 300 ATATTTCTAG GAGCCGAAGA GATGACAGAT GAGATATTGT TCTAATGATT AGTTATTCAG 82241 .......... .......... .......... .......... .......... .......... 300 ATAAATGGTT CAGATGTAGC TTGTCTCACA TCTTATTTCT TCTCTAAAAG ACACAAGAAC 82181 .......... .......... .......... .......... .......... .......... 300 AAGTAAATAA AGGTGTAGAA CTTCAATTCT TTCCTTTTGA GCTAATCATT CATCTTCTCC 82121 .......... .......... .......... .......... .......... .......... 300 ATTAGGCTAA TCTAGATTAA CAATCCCCAC TTCTTCGACT CTTCTCTTAC GGATTTAATA 82061 .......... .......... .......... .......... .......... .......... 300 AAAAGGACGA AGGAGAAAGA ACAAAAGAAA GCTAAAAAAA TCTTTTAGTT GCTGTACTAT 82001 .......... .......... .......... .......... .......... .......... 300 TCTCTTACTG TCAACTCGAA GATTAAAAAA TTTAGCTTTC TTTTTCTTTT CCTGATCAAA 81941 .......... .......... .......... .......... .......... .......... 300 AAGAAAATTA CGATTTGACA GTAAGGGAAT GCTAAACCTG ATCCTTGGAC CAGATCTAGT 81881 .......... .......... .......... .......... .......... .......... 300 AATTATTTCC AATAAATGGA AATTATATAT AGATCTTGCC TACCTTTTCC CCCCAGAGTC 81821 .......... .......... .......... .......... .......... .......... 300 TTCACAATTT AGAAATGCAA ATAGTTTCCC CCCACTTGTT TCTTTTATGT TCTTTACTTA 81761 .......... .......... .......... .......... .......... .......... 300 TTTACTTTTA ACTAAGCCAG AATTATGGTA TGTTATTTTG ATTTGCAGCT GTAATTTAAA 81701 .......... .......... .......... .......... .......... .......... 300 AATAATATTT GCACCATCTA CCTCTATTCA TATTCTGTAC TATGTACAAC ATCATGTGCT 81641 .......... .......... .......... .......... .......... .......... 300 TCTCAGACCT CAATATTATT TGTGTTGTCA TGTTCTTCTA GCTTTGAAAG ATATATCTAT 81581 .......... .......... .......... .......... .......... .......... 300 TTCTTACTTT TCTCAAGTAT GATTTGACCT CTTAGATCTG CGATCAGCTT GTGTTTTCAC 81521 .......... .......... .......... .......... .......... .......... 300 TGACAAAGTA CTTTGACTCA GGGGAGATAT GAGATTATGA GAAATTACAT GCCTAAAGTT 81461 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .GGGAGATAT GAGATTATGA GAAATTACAT GCCTAAAGTT 339 GGCTCACTTG GGCTGGATAT G 81440 |||||||||| |||||||||| | GGCTCACTTG GGCTGGATAT G 360 hqPGS_C08HBa0251H04.1-_SGN-E270608+ (83620 83522,83328 83221,82773 82681,81499 81440) ******************************************************************************** EST sequence 124 +strand 391 n (File: SGN-E284559+) 1 ACTTGATGAG CAGGATAGGC ACTGAACATG AAAAGTTTGG TTTCGAGTTT GGAACCCTGC 61 GACCCATGAA GTATGATCAA ATAGCTGACT TGCTAAATGG TATTGCTGAG CGGTTTGATT 121 GGGAAAAAGT AATGGAGGGT GACAAGATTA TTGGCCTGAA ACAGGGAAAG CAAAGCATAT 181 CATTAGAACC TGGTGGTCAG TTTGAGCTTA GTGGTGCACC ACTTGAAACA CTGCATCAAA 241 CTTGTGCAGA GGTTAATTCA CATCTTTACC AGGTTAAAGC TGTTGCAGAA GAGATGGGAA 301 TTGGATTCTT AGGAACTGGA TTCCAGCCAA AGTGGGGGCT GAAAGATATA CCAATAATGC 361 CGAAGGGGAG ATATGAGATT ATGAGAAATT A Predicted gene structure (within gDNA segment 84285 to 81820): Exon 1 83685 83522 ( 164 n); cDNA 1 164 ( 164 n); score: 1.000 Intron 1 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 2 83328 83221 ( 108 n); cDNA 165 272 ( 108 n); score: 1.000 Intron 2 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 82773 82681 ( 93 n); cDNA 273 365 ( 93 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E284559+ 1.000 365 0.934 C PGS_C08HBa0251H04.1-_SGN-E284559+ (83685 83522,83328 83221,82773 82681) Alignment (genomic DNA sequence = upper lines): ACTTGATGAG CAGGATAGGC ACTGAACATG AAAAGTTTGG TTTCGAGTTT GGAACCCTGC 83626 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTTGATGAG CAGGATAGGC ACTGAACATG AAAAGTTTGG TTTCGAGTTT GGAACCCTGC 60 GACCCATGAA GTATGATCAA ATAGCTGACT TGCTAAATGG TATTGCTGAG CGGTTTGATT 83566 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACCCATGAA GTATGATCAA ATAGCTGACT TGCTAAATGG TATTGCTGAG CGGTTTGATT 120 GGGAAAAAGT AATGGAGGGT GACAAGATTA TTGGCCTGAA ACAGGTAACG TGAACTTGGT 83506 |||||||||| |||||||||| |||||||||| |||||||||| |||| GGGAAAAAGT AATGGAGGGT GACAAGATTA TTGGCCTGAA ACAG...... .......... 164 GCAAACTTTG GTTTAAAATA TTCTAGGTTG AATTTGTCAT GGCTCCATAC AATGTATTAT 83446 .......... .......... .......... .......... .......... .......... 164 GCTACTTACC ATGAATAGAA TCATAATTCC TGGTTCATTT TTTTCCTCAA GTGCAAGTCT 83386 .......... .......... .......... .......... .......... .......... 164 GTAGTAAGTA GTAATTACTT TAGCATAATT GGTGGATAAT TTGCACTGTC TTCGCAGGGA 83326 ||| .......... .......... .......... .......... .......... .......GGA 167 AAGCAAAGCA TATCATTAGA ACCTGGTGGT CAGTTTGAGC TTAGTGGTGC ACCACTTGAA 83266 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCAAAGCA TATCATTAGA ACCTGGTGGT CAGTTTGAGC TTAGTGGTGC ACCACTTGAA 227 ACACTGCATC AAACTTGTGC AGAGGTTAAT TCACATCTTT ACCAGGTTTG TACTTTTGAA 83206 |||||||||| |||||||||| |||||||||| |||||||||| ||||| ACACTGCATC AAACTTGTGC AGAGGTTAAT TCACATCTTT ACCAG..... .......... 272 ACTCCATCAG TTTCCCTTTT AATGAAATAG TGGGCAATTA AGATCTTTCT AACTATACAT 83146 .......... .......... .......... .......... .......... .......... 272 AGTCTGCACT GGGGAAATGT AAGCGAACTT TAATTATGCG ATAACATGGC TTGGAATAAA 83086 .......... .......... .......... .......... .......... .......... 272 AGGGAATCTC ATTTTTTTGA GTTTGAGAAA TCCTGCAAAT GTGTCGGCCA TTTTTGCTTC 83026 .......... .......... .......... .......... .......... .......... 272 AGGTGGTTGA TGACTTATTA CACTAGGGAT TCTTTACCCA GTATTCTGGT TCTTCTGATC 82966 .......... .......... .......... .......... .......... .......... 272 AGAGTCTCTA GTTGTATAAG AAAATGTTGC TTCAAAAGAA CATTGTTGGG GTGAAAGTAA 82906 .......... .......... .......... .......... .......... .......... 272 AGTTATGACT TCCTGACTGT CTTGTCGATG AACATTTGGA GGCAAGAAAA CCGAAGCTAT 82846 .......... .......... .......... .......... .......... .......... 272 TCTCACAGGA GAAATATTCA CGGATAAATT TAAGTTTTGC TGATATGTAA TAGTTTTCGT 82786 .......... .......... .......... .......... .......... .......... 272 CTAATGGTAC AGGTTAAAGC TGTTGCAGAA GAGATGGGAA TTGGATTCTT AGGAACTGGA 82726 |||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ..GTTAAAGC TGTTGCAGAA GAGATGGGAA TTGGATTCTT AGGAACTGGA 320 TTCCAGCCAA AGTGGGGGCT GAAAGATATA CCAATAATGC CGAAG 82681 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TTCCAGCCAA AGTGGGGGCT GAAAGATATA CCAATAATGC CGAAG 365 hqPGS_C08HBa0251H04.1-_SGN-E284559+ (83685 83522,83328 83221,82773 82681) ******************************************************************************** EST sequence 92 +strand 673 n (File: SGN-E339388+) 1 ACAAAGCATG GCCTTGATGT CTCAGGCAGG TTCTTCACAT TGCATTTACT CTGAAAAGGT 61 AAGATGTATC AGTGGACATA GAAGCATCAT TAACAATATG GATATGTTCA CAATGCGGGA 121 GATATGCTTT GGCGTGGATA TTACTTCACG CAATGCCTCA AGACGAGTGC AAGGGAATTA 181 TTTGAATCAC ATAGGACTGG GAAGTAGACC TGGAGACCTG ACAATTGTAG CTGCAAGTCC 241 TCCAACAGAG GATGCTGTTG TTGCAGCAGA GCCGTTAACA AAAGAACATC TTGTAGGATA 301 TCTTGCTTCT GGATGCAAAT CCAAAGAAAA GTGGAGGATA GGCACTGAAC ATGAAAAGTG 361 TGGTTTCCAG TTTGGAACCC TGCGACCCAT GAAGTATGAT CAAATAGCTG ACTTGCTAAA 421 TGGTATTGCT GAGCGGCTTG ATTGGGAAAA AGTAATGGAG GGTGACAAGA TTATTGGCCT 481 GAAACAGGGA AAGCAAAGCA TATCATTAGA ACCTGGTGGT CAGTTTGAGC TTAGAGGTGC 541 ACCACTAGAC ACACTGCATC AAACTTGAGC AGAGGTTAAT GCACATCTTT ACCAGGTTAA 601 AGCTGTTGCA GAAGAGATGG GAATTGGATT CTTAGGAACT GGATTCCAGC CAAAGTGAGG 661 GCTGAAAGAT ATA Predicted gene structure (within gDNA segment 85310 to 81942): Exon 1 84710 84375 ( 336 n); cDNA 1 336 ( 336 n); score: 0.985 Intron 1 84374 83673 ( 702 n); Pd: 0.992 (s: 0.98), Pa: 0.746 (s: 0.96) Exon 2 83672 83522 ( 151 n); cDNA 337 487 ( 151 n); score: 0.980 Intron 2 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 0.98) Exon 3 83328 83221 ( 108 n); cDNA 488 595 ( 108 n); score: 0.954 Intron 3 83220 82774 ( 447 n); Pd: 0.987 (s: 0.92), Pa: 0.999 (s: 1.00) Exon 4 82773 82696 ( 78 n); cDNA 596 673 ( 78 n); score: 0.987 MATCH C08HBa0251H04.1- SGN-E339388+ 0.979 673 1.000 C PGS_C08HBa0251H04.1-_SGN-E339388+ (84710 84375,83672 83522,83328 83221,82773 82696) Alignment (genomic DNA sequence = upper lines): ACAAAGCATG GCCTTGATGT CTCAGGCAGG TTCTTCACAT TGCATTTACT CTGAAAAGGT 84651 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACAAAGCATG GCCTTGATGT CTCAGGCAGG TTCTTCACAT TGCATTTACT CTGAAAAGGT 60 AAGATGTATC AGTGGACATA GAAGCATCAT TAACAATATG GATATGTTCA GAATGCGGGA 84591 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AAGATGTATC AGTGGACATA GAAGCATCAT TAACAATATG GATATGTTCA CAATGCGGGA 120 GATATGCTTT GGCGTGGATA TTTCTTCACG CAATGCCTCA AGACGAGTGC AAGGGAATTA 84531 |||||||||| |||||||||| || ||||||| |||||||||| |||||||||| |||||||||| GATATGCTTT GGCGTGGATA TTACTTCACG CAATGCCTCA AGACGAGTGC AAGGGAATTA 180 TTTGAATCAC ATAGGAGTGG GAAGTAGACG TGGAGACCTG ACAATTGTAG CTGCAAGTCC 84471 |||||||||| |||||| ||| ||||||||| |||||||||| |||||||||| |||||||||| TTTGAATCAC ATAGGACTGG GAAGTAGACC TGGAGACCTG ACAATTGTAG CTGCAAGTCC 240 TCCAACAGAG GATGCTGTTG TTGCAGCAGA GCCGTTAACA AAAGAAGATC TTGTAGGATA 84411 |||||||||| |||||||||| |||||||||| |||||||||| |||||| ||| |||||||||| TCCAACAGAG GATGCTGTTG TTGCAGCAGA GCCGTTAACA AAAGAACATC TTGTAGGATA 300 TCTTGCTTCT GGATGCAAAT CCAAAGAAAA GTGGAGGTTC GAAGTTAAAC TTTTCTTAGT 84351 |||||||||| |||||||||| |||||||||| |||||| TCTTGCTTCT GGATGCAAAT CCAAAGAAAA GTGGAG.... .......... .......... 336 GCTCTTAAAT TTCAGTTGCA TATAGTTAAC CAAGAATATG AATATTCATT CTCTCGTTTG 84291 .......... .......... .......... .......... .......... .......... 336 GCCTTTATAA GTAGAATAGT CTGCTCATTC ACCACATATA TGCCTTGATT GTCTTTCAAG 84231 .......... .......... .......... .......... .......... .......... 336 TATGTCGAGT AATTCATGAA TCAATTCACA TCGCTTGTGT ATTTTAATAT ACGGCTATTT 84171 .......... .......... .......... .......... .......... .......... 336 TTTATGTAGT TATTCTTTCT TCAGCTTCTG TTGTAGTGTA TGATACGGTA GATGTATTAT 84111 .......... .......... .......... .......... .......... .......... 336 ATACTTTTTA CATGTCATTA TTGTGGTTAT TTCTCATTTT CCATTTCTAA GAAATATAAA 84051 .......... .......... .......... .......... .......... .......... 336 TATCATGGGC AAATTCTCAC CGTCATGCAT TCAAGATGTG GATACTAGGG AACTAGGACA 83991 .......... .......... .......... .......... .......... .......... 336 TGATGCAGCT CTTTTCATTG AACTAACAAG TTGTACCATT TGGGCAACTT GAACACTGGA 83931 .......... .......... .......... .......... .......... .......... 336 AACATTAATC AATTTTTCCT AGGACACTGC TGTCTCTAAT TTTGTTGCAA ATTTTGAATT 83871 .......... .......... .......... .......... .......... .......... 336 GTTGAAATTT CGGTAATACA ATTTTTGAAC ATGGAGTTGG GAGCCATTCC CTGGATCAGG 83811 .......... .......... .......... .......... .......... .......... 336 TATTTTTTCA TAAATGTGGT AGCTAGGATT CTGCCTCAGT CATTATTTGG ATAGTTTGTT 83751 .......... .......... .......... .......... .......... .......... 336 TTACTTAACT GTGCGTCTGG ATCTAGACCA TAATAATCAG GTTCAACTTT TCTTGTTCTC 83691 .......... .......... .......... .......... .......... .......... 336 TCAAAACTTG ATGAGCAGGA TAGGCACTGA ACATGAAAAG TTTGGTTTCG AGTTTGGAAC 83631 || |||||||||| |||||||||| | ||||||| |||||||||| .......... ........GA TAGGCACTGA ACATGAAAAG TGTGGTTTCC AGTTTGGAAC 378 CCTGCGACCC ATGAAGTATG ATCAAATAGC TGACTTGCTA AATGGTATTG CTGAGCGGTT 83571 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| | CCTGCGACCC ATGAAGTATG ATCAAATAGC TGACTTGCTA AATGGTATTG CTGAGCGGCT 438 TGATTGGGAA AAAGTAATGG AGGGTGACAA GATTATTGGC CTGAAACAGG TAACGTGAAC 83511 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| TGATTGGGAA AAAGTAATGG AGGGTGACAA GATTATTGGC CTGAAACAG. .......... 487 TTGGTGCAAA CTTTGGTTTA AAATATTCTA GGTTGAATTT GTCATGGCTC CATACAATGT 83451 .......... .......... .......... .......... .......... .......... 487 ATTATGCTAC TTACCATGAA TAGAATCATA ATTCCTGGTT CATTTTTTTC CTCAAGTGCA 83391 .......... .......... .......... .......... .......... .......... 487 AGTCTGTAGT AAGTAGTAAT TACTTTAGCA TAATTGGTGG ATAATTTGCA CTGTCTTCGC 83331 .......... .......... .......... .......... .......... .......... 487 AGGGAAAGCA AAGCATATCA TTAGAACCTG GTGGTCAGTT TGAGCTTAGT GGTGCACCAC 83271 |||||||| |||||||||| |||||||||| |||||||||| ||||||||| |||||||||| ..GGAAAGCA AAGCATATCA TTAGAACCTG GTGGTCAGTT TGAGCTTAGA GGTGCACCAC 545 TTGAAACACT GCATCAAACT TGTGCAGAGG TTAATTCACA TCTTTACCAG GTTTGTACTT 83211 | || ||||| |||||||||| || ||||||| ||||| |||| |||||||||| TAGACACACT GCATCAAACT TGAGCAGAGG TTAATGCACA TCTTTACCAG .......... 595 TTGAAACTCC ATCAGTTTCC CTTTTAATGA AATAGTGGGC AATTAAGATC TTTCTAACTA 83151 .......... .......... .......... .......... .......... .......... 595 TACATAGTCT GCACTGGGGA AATGTAAGCG AACTTTAATT ATGCGATAAC ATGGCTTGGA 83091 .......... .......... .......... .......... .......... .......... 595 ATAAAAGGGA ATCTCATTTT TTTGAGTTTG AGAAATCCTG CAAATGTGTC GGCCATTTTT 83031 .......... .......... .......... .......... .......... .......... 595 GCTTCAGGTG GTTGATGACT TATTACACTA GGGATTCTTT ACCCAGTATT CTGGTTCTTC 82971 .......... .......... .......... .......... .......... .......... 595 TGATCAGAGT CTCTAGTTGT ATAAGAAAAT GTTGCTTCAA AAGAACATTG TTGGGGTGAA 82911 .......... .......... .......... .......... .......... .......... 595 AGTAAAGTTA TGACTTCCTG ACTGTCTTGT CGATGAACAT TTGGAGGCAA GAAAACCGAA 82851 .......... .......... .......... .......... .......... .......... 595 GCTATTCTCA CAGGAGAAAT ATTCACGGAT AAATTTAAGT TTTGCTGATA TGTAATAGTT 82791 .......... .......... .......... .......... .......... .......... 595 TTCGTCTAAT GGTACAGGTT AAAGCTGTTG CAGAAGAGAT GGGAATTGGA TTCTTAGGAA 82731 ||| |||||||||| |||||||||| |||||||||| |||||||||| .......... .......GTT AAAGCTGTTG CAGAAGAGAT GGGAATTGGA TTCTTAGGAA 638 CTGGATTCCA GCCAAAGTGG GGGCTGAAAG ATATA 82696 |||||||||| ||||||||| |||||||||| ||||| CTGGATTCCA GCCAAAGTGA GGGCTGAAAG ATATA 673 hqPGS_C08HBa0251H04.1-_SGN-E339388+ (84710 84375,83672 83522,83328 83221,82773 82696) ******************************************************************************** EST sequence 111 +strand 340 n (File: SGN-E200628+) 1 TCTTGTAGGA TATCTTGCTT CTGGATGCAA ATCCAAAGAA AAGTGGAGGA TAGGCACTGA 61 ACATGAAAAG TTTGGTTTCG AGTTTGGAAC CCTGCGACCC ATGAAGTATG ATCAAATAGC 121 TGACTTGCTA AATGGTATTG CTGAGCGGTT TGATTGGGAA AAAGTAATGG AGGGTGACAA 181 GATTATTGGC CTGAAACAGG GAAAGCAAAG CATATCATTA GAACCTGGTG GTCAGTTTGA 241 GCTTAGTGGT GCACCACTTG AAACACTGCA TCAAACTTGT GCAGAGGTTA ATTCACATCT 301 TTACCAGGTT AAAGCTGTTG CAAGAAGAGA TGGGGAATTG Predicted gene structure (within gDNA segment 85022 to 81708): Exon 1 84422 84375 ( 48 n); cDNA 1 48 ( 48 n); score: 1.000 Intron 1 84374 83673 ( 702 n); Pd: 0.992 (s: 1.00), Pa: 0.746 (s: 1.00) Exon 2 83672 83522 ( 151 n); cDNA 49 199 ( 151 n); score: 1.000 Intron 2 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 3 83328 83221 ( 108 n); cDNA 200 307 ( 108 n); score: 1.000 Intron 3 83220 82774 ( 447 n); Pd: 0.987 (s: 1.00), Pa: 0.999 (s: 0) Exon 4 82773 82748 ( 26 n); cDNA 308 334 ( 27 n); score: 0.904 MATCH C08HBa0251H04.1- SGN-E200628+ 1.000 333 0.979 C PGS_C08HBa0251H04.1-_SGN-E200628+ (84422 84375,83672 83522,83328 83221,82773 82748) Alignment (genomic DNA sequence = upper lines): TCTTGTAGGA TATCTTGCTT CTGGATGCAA ATCCAAAGAA AAGTGGAGGT TCGAAGTTAA 84363 |||||||||| |||||||||| |||||||||| |||||||||| |||||||| TCTTGTAGGA TATCTTGCTT CTGGATGCAA ATCCAAAGAA AAGTGGAG.. .......... 48 ACTTTTCTTA GTGCTCTTAA ATTTCAGTTG CATATAGTTA ACCAAGAATA TGAATATTCA 84303 .......... .......... .......... .......... .......... .......... 48 TTCTCTCGTT TGGCCTTTAT AAGTAGAATA GTCTGCTCAT TCACCACATA TATGCCTTGA 84243 .......... .......... .......... .......... .......... .......... 48 TTGTCTTTCA AGTATGTCGA GTAATTCATG AATCAATTCA CATCGCTTGT GTATTTTAAT 84183 .......... .......... .......... .......... .......... .......... 48 ATACGGCTAT TTTTTATGTA GTTATTCTTT CTTCAGCTTC TGTTGTAGTG TATGATACGG 84123 .......... .......... .......... .......... .......... .......... 48 TAGATGTATT ATATACTTTT TACATGTCAT TATTGTGGTT ATTTCTCATT TTCCATTTCT 84063 .......... .......... .......... .......... .......... .......... 48 AAGAAATATA AATATCATGG GCAAATTCTC ACCGTCATGC ATTCAAGATG TGGATACTAG 84003 .......... .......... .......... .......... .......... .......... 48 GGAACTAGGA CATGATGCAG CTCTTTTCAT TGAACTAACA AGTTGTACCA TTTGGGCAAC 83943 .......... .......... .......... .......... .......... .......... 48 TTGAACACTG GAAACATTAA TCAATTTTTC CTAGGACACT GCTGTCTCTA ATTTTGTTGC 83883 .......... .......... .......... .......... .......... .......... 48 AAATTTTGAA TTGTTGAAAT TTCGGTAATA CAATTTTTGA ACATGGAGTT GGGAGCCATT 83823 .......... .......... .......... .......... .......... .......... 48 CCCTGGATCA GGTATTTTTT CATAAATGTG GTAGCTAGGA TTCTGCCTCA GTCATTATTT 83763 .......... .......... .......... .......... .......... .......... 48 GGATAGTTTG TTTTACTTAA CTGTGCGTCT GGATCTAGAC CATAATAATC AGGTTCAACT 83703 .......... .......... .......... .......... .......... .......... 48 TTTCTTGTTC TCTCAAAACT TGATGAGCAG GATAGGCACT GAACATGAAA AGTTTGGTTT 83643 |||||||||| |||||||||| |||||||||| .......... .......... .......... GATAGGCACT GAACATGAAA AGTTTGGTTT 78 CGAGTTTGGA ACCCTGCGAC CCATGAAGTA TGATCAAATA GCTGACTTGC TAAATGGTAT 83583 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGAGTTTGGA ACCCTGCGAC CCATGAAGTA TGATCAAATA GCTGACTTGC TAAATGGTAT 138 TGCTGAGCGG TTTGATTGGG AAAAAGTAAT GGAGGGTGAC AAGATTATTG GCCTGAAACA 83523 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTGAGCGG TTTGATTGGG AAAAAGTAAT GGAGGGTGAC AAGATTATTG GCCTGAAACA 198 GGTAACGTGA ACTTGGTGCA AACTTTGGTT TAAAATATTC TAGGTTGAAT TTGTCATGGC 83463 | G......... .......... .......... .......... .......... .......... 199 TCCATACAAT GTATTATGCT ACTTACCATG AATAGAATCA TAATTCCTGG TTCATTTTTT 83403 .......... .......... .......... .......... .......... .......... 199 TCCTCAAGTG CAAGTCTGTA GTAAGTAGTA ATTACTTTAG CATAATTGGT GGATAATTTG 83343 .......... .......... .......... .......... .......... .......... 199 CACTGTCTTC GCAGGGAAAG CAAAGCATAT CATTAGAACC TGGTGGTCAG TTTGAGCTTA 83283 |||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ....GGAAAG CAAAGCATAT CATTAGAACC TGGTGGTCAG TTTGAGCTTA 245 GTGGTGCACC ACTTGAAACA CTGCATCAAA CTTGTGCAGA GGTTAATTCA CATCTTTACC 83223 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGGTGCACC ACTTGAAACA CTGCATCAAA CTTGTGCAGA GGTTAATTCA CATCTTTACC 305 AGGTTTGTAC TTTTGAAACT CCATCAGTTT CCCTTTTAAT GAAATAGTGG GCAATTAAGA 83163 || AG........ .......... .......... .......... .......... .......... 307 TCTTTCTAAC TATACATAGT CTGCACTGGG GAAATGTAAG CGAACTTTAA TTATGCGATA 83103 .......... .......... .......... .......... .......... .......... 307 ACATGGCTTG GAATAAAAGG GAATCTCATT TTTTTGAGTT TGAGAAATCC TGCAAATGTG 83043 .......... .......... .......... .......... .......... .......... 307 TCGGCCATTT TTGCTTCAGG TGGTTGATGA CTTATTACAC TAGGGATTCT TTACCCAGTA 82983 .......... .......... .......... .......... .......... .......... 307 TTCTGGTTCT TCTGATCAGA GTCTCTAGTT GTATAAGAAA ATGTTGCTTC AAAAGAACAT 82923 .......... .......... .......... .......... .......... .......... 307 TGTTGGGGTG AAAGTAAAGT TATGACTTCC TGACTGTCTT GTCGATGAAC ATTTGGAGGC 82863 .......... .......... .......... .......... .......... .......... 307 AAGAAAACCG AAGCTATTCT CACAGGAGAA ATATTCACGG ATAAATTTAA GTTTTGCTGA 82803 .......... .......... .......... .......... .......... .......... 307 TATGTAATAG TTTTCGTCTA ATGGTACAGG TTAAAGCTGT TGC-AGAAGA GATGGG 82748 | |||||||||| ||| |||||| |||||| .......... .......... .........G TTAAAGCTGT TGCAAGAAGA GATGGG 334 hqPGS_C08HBa0251H04.1-_SGN-E200628+ (84422 84375,83672 83522,83328 83221,82773 82748) ******************************************************************************** EST sequence 109 +strand 573 n (File: SGN-E204027+) 1 TACAAAGTCT TAATTTTTCC AGTTTACTGA GCCAAGGCAC AAAGCATGGC CTTGATGTCT 61 CAGGCAGGTT CTTCACATTG CATTTACTCT GAAAAGGTAA GATGTATCAG TGGACATAGA 121 AGCATCATTA ACAATATGGA TATGTTCAGA ATGCGGGAGA TATGCTTTGG CGTGGATATT 181 TCTTCACGCA ATGCCTCAAG ACGAGTGCAA GGGAATTATT TGAATCACAT AGGAGTGGGA 241 AGTAGACGTG GAGACCTGAC AATTGTAGCT GCAAGTCCTC CAACAGAGGA TGCTGTTGTT 301 GCAGCAGAGC CGTTAACAAA AGAAGATCTT GTAGGATATC TTGCTTCTGG ATGCAAATCC 361 AAAGAAAAGT GGAGGATAGG CACTGAACAT GAAAAGTTTG GTTTCGAGTT TGGAACCCTG 421 CGACCCATGA AGTATGATCA AATAGCTGAC TTGCTAAATG GTATTGCTGA GCGGTTTGAT 481 TGGGAAAAAG TAATGGAGGG TGACAAGATT ATTGGCCTGA AACAGGGAAA GCAAAGCATA 541 TCATTAGAAC CTGGTGGTCA GTTTGAGCTT AGT Predicted gene structure (within gDNA segment 86275 to 82671): Exon 1 85675 85640 ( 36 n); cDNA 1 36 ( 36 n); score: 1.000 Intron 1 85639 84713 ( 927 n); Pd: 0.935 (s: 0), Pa: 0.993 (s: 1.00) Exon 2 84712 84375 ( 338 n); cDNA 37 374 ( 338 n); score: 1.000 Intron 2 84374 83673 ( 702 n); Pd: 0.992 (s: 1.00), Pa: 0.746 (s: 1.00) Exon 3 83672 83522 ( 151 n); cDNA 375 525 ( 151 n); score: 1.000 Intron 3 83521 83329 ( 193 n); Pd: 0.994 (s: 1.00), Pa: 0.994 (s: 1.00) Exon 4 83328 83281 ( 48 n); cDNA 526 573 ( 48 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E204027+ 1.000 573 1.000 C PGS_C08HBa0251H04.1-_SGN-E204027+ (85675 85640,84712 84375,83672 83522,83328 83281) Alignment (genomic DNA sequence = upper lines): TACAAAGTCT TAATTTTTCC AGTTTACTGA GCCAAGGTAT CACCTTTTTG TCACCTTTTA 85616 |||||||||| |||||||||| |||||||||| |||||| TACAAAGTCT TAATTTTTCC AGTTTACTGA GCCAAG.... .......... .......... 36 TGTTGGTTTC AGTTAGTTTT GTTAATCAAG ATTCAATTTT TCTGACATAC CCATGTTTCT 85556 .......... .......... .......... .......... .......... .......... 36 TTTGTATGCT TTTGTTGCAT TTCTGTTTTC TTCATGAAGT TGTTTTGGTT AATCAAGATT 85496 .......... .......... .......... .......... .......... .......... 36 CAGTTTTTCT GAAGCCCCAT GATTTTTTTC TTTTAGTTTT GGTTTGATGG TTTTGTTAGT 85436 .......... .......... .......... .......... .......... .......... 36 TGTGTTGTAA TTCTTGTTTG TTGCCATATG GGGGTTGATA GTATTGATTA AAGATTCAAA 85376 .......... .......... .......... .......... .......... .......... 36 ATTTATACAT GTGGGTTGTG GTAGTCTGAT TGAATGACAT GCATAGTAAA CTAGAAAGAA 85316 .......... .......... .......... .......... .......... .......... 36 GAAAGAGATA AATGCTTTAG ATAATGTGAA CAAGACTTGT ATTATAGGTT AGACTGCTTT 85256 .......... .......... .......... .......... .......... .......... 36 ACAAGTTTCA GTTTCTCGTG TTCTTCTGAG TAGTTTTAAG TGTTGAATGA AATGGCATTG 85196 .......... .......... .......... .......... .......... .......... 36 TGTAAGATTG CATTTGATGG TCTGGGATTC AGGTATGATT GATGCAGGAC AGGAATATCA 85136 .......... .......... .......... .......... .......... .......... 36 AGCCACATTT TCTTTATTGT AGCTGTGTTT CGATATGGAA TGAGATGGGG GTGGGGTGGG 85076 .......... .......... .......... .......... .......... .......... 36 GGAGTTTACA GCATGTAGAA TTGTAGAGCA CTGTAATGAA AATTTCTAGA TTATTGCAAT 85016 .......... .......... .......... .......... .......... .......... 36 TCGAATCTTA TGTTGCTTAT ATAGTACATC TTAATTAACT TTAATATTTG TTCGGATTGG 84956 .......... .......... .......... .......... .......... .......... 36 TGATTGAGCC TGCTGGGTAG TTGGGTTGGC CCTATCCTAT TGGTGTATAA CTATGCTCAA 84896 .......... .......... .......... .......... .......... .......... 36 TGAAGATGCG AGAGGTTCAT TTGACGTTTC CCATCAAAAG TACTCTTCAA TCATTATTTA 84836 .......... .......... .......... .......... .......... .......... 36 GTTATCCTCT TCTGTTACTA AATGTAATAC ACAAGTTAAA CAATAATCTG AATTCCTTGT 84776 .......... .......... .......... .......... .......... .......... 36 CTACTCAAAT GGCATACATT CTAGTTAAAA GTGGTTGACA TCAGTTTTTC CTGTGATATT 84716 .......... .......... .......... .......... .......... .......... 36 CAGGCACAAA GCATGGCCTT GATGTCTCAG GCAGGTTCTT CACATTGCAT TTACTCTGAA 84656 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GCACAAA GCATGGCCTT GATGTCTCAG GCAGGTTCTT CACATTGCAT TTACTCTGAA 93 AAGGTAAGAT GTATCAGTGG ACATAGAAGC ATCATTAACA ATATGGATAT GTTCAGAATG 84596 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGGTAAGAT GTATCAGTGG ACATAGAAGC ATCATTAACA ATATGGATAT GTTCAGAATG 153 CGGGAGATAT GCTTTGGCGT GGATATTTCT TCACGCAATG CCTCAAGACG AGTGCAAGGG 84536 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGGGAGATAT GCTTTGGCGT GGATATTTCT TCACGCAATG CCTCAAGACG AGTGCAAGGG 213 AATTATTTGA ATCACATAGG AGTGGGAAGT AGACGTGGAG ACCTGACAAT TGTAGCTGCA 84476 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATTATTTGA ATCACATAGG AGTGGGAAGT AGACGTGGAG ACCTGACAAT TGTAGCTGCA 273 AGTCCTCCAA CAGAGGATGC TGTTGTTGCA GCAGAGCCGT TAACAAAAGA AGATCTTGTA 84416 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTCCTCCAA CAGAGGATGC TGTTGTTGCA GCAGAGCCGT TAACAAAAGA AGATCTTGTA 333 GGATATCTTG CTTCTGGATG CAAATCCAAA GAAAAGTGGA GGTTCGAAGT TAAACTTTTC 84356 |||||||||| |||||||||| |||||||||| |||||||||| | GGATATCTTG CTTCTGGATG CAAATCCAAA GAAAAGTGGA G......... .......... 374 TTAGTGCTCT TAAATTTCAG TTGCATATAG TTAACCAAGA ATATGAATAT TCATTCTCTC 84296 .......... .......... .......... .......... .......... .......... 374 GTTTGGCCTT TATAAGTAGA ATAGTCTGCT CATTCACCAC ATATATGCCT TGATTGTCTT 84236 .......... .......... .......... .......... .......... .......... 374 TCAAGTATGT CGAGTAATTC ATGAATCAAT TCACATCGCT TGTGTATTTT AATATACGGC 84176 .......... .......... .......... .......... .......... .......... 374 TATTTTTTAT GTAGTTATTC TTTCTTCAGC TTCTGTTGTA GTGTATGATA CGGTAGATGT 84116 .......... .......... .......... .......... .......... .......... 374 ATTATATACT TTTTACATGT CATTATTGTG GTTATTTCTC ATTTTCCATT TCTAAGAAAT 84056 .......... .......... .......... .......... .......... .......... 374 ATAAATATCA TGGGCAAATT CTCACCGTCA TGCATTCAAG ATGTGGATAC TAGGGAACTA 83996 .......... .......... .......... .......... .......... .......... 374 GGACATGATG CAGCTCTTTT CATTGAACTA ACAAGTTGTA CCATTTGGGC AACTTGAACA 83936 .......... .......... .......... .......... .......... .......... 374 CTGGAAACAT TAATCAATTT TTCCTAGGAC ACTGCTGTCT CTAATTTTGT TGCAAATTTT 83876 .......... .......... .......... .......... .......... .......... 374 GAATTGTTGA AATTTCGGTA ATACAATTTT TGAACATGGA GTTGGGAGCC ATTCCCTGGA 83816 .......... .......... .......... .......... .......... .......... 374 TCAGGTATTT TTTCATAAAT GTGGTAGCTA GGATTCTGCC TCAGTCATTA TTTGGATAGT 83756 .......... .......... .......... .......... .......... .......... 374 TTGTTTTACT TAACTGTGCG TCTGGATCTA GACCATAATA ATCAGGTTCA ACTTTTCTTG 83696 .......... .......... .......... .......... .......... .......... 374 TTCTCTCAAA ACTTGATGAG CAGGATAGGC ACTGAACATG AAAAGTTTGG TTTCGAGTTT 83636 ||||||| |||||||||| |||||||||| |||||||||| .......... .......... ...GATAGGC ACTGAACATG AAAAGTTTGG TTTCGAGTTT 411 GGAACCCTGC GACCCATGAA GTATGATCAA ATAGCTGACT TGCTAAATGG TATTGCTGAG 83576 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAACCCTGC GACCCATGAA GTATGATCAA ATAGCTGACT TGCTAAATGG TATTGCTGAG 471 CGGTTTGATT GGGAAAAAGT AATGGAGGGT GACAAGATTA TTGGCCTGAA ACAGGTAACG 83516 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| CGGTTTGATT GGGAAAAAGT AATGGAGGGT GACAAGATTA TTGGCCTGAA ACAG...... 525 TGAACTTGGT GCAAACTTTG GTTTAAAATA TTCTAGGTTG AATTTGTCAT GGCTCCATAC 83456 .......... .......... .......... .......... .......... .......... 525 AATGTATTAT GCTACTTACC ATGAATAGAA TCATAATTCC TGGTTCATTT TTTTCCTCAA 83396 .......... .......... .......... .......... .......... .......... 525 GTGCAAGTCT GTAGTAAGTA GTAATTACTT TAGCATAATT GGTGGATAAT TTGCACTGTC 83336 .......... .......... .......... .......... .......... .......... 525 TTCGCAGGGA AAGCAAAGCA TATCATTAGA ACCTGGTGGT CAGTTTGAGC TTAGT 83281 ||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| .......GGA AAGCAAAGCA TATCATTAGA ACCTGGTGGT CAGTTTGAGC TTAGT 573 hqPGS_C08HBa0251H04.1-_SGN-E204027+ (85675 85640,84712 84375,83672 83522,83328 83281) ******************************************************************************** EST sequence 63 +strand 704 n (File: SGN-E348164+) 1 TCTCTCTTTA TAGCTAAACA AAAATTAAAG AAATGGGAGT GATAAAGCTC TAGCCGCCTC 61 AGCACACCAA AATCCTAATC ATTTTGCTGA TACCCAATTG TCTCTATATA TATATTGGAA 121 GGTTTTCTTT CAATTTTCAG TCAATTCTAT AGAACAAACC CTACAAAGTC TTAATTTTTC 181 CAGTTTACTG AGCCAAGGCA CAAAGCATGG CCTTGATGTC TCAGGCAGGT TCTTCACATT 241 GCATTTACTC TGAAAAGGTA AGATGTATCA GTGGACATAG AAGCATCATT AACAATATGG 301 ATATGTTCAG AATGCGGGAG ATATGCTTTG GCGTGGATAT TTCTTCACGC AATGCCTCAA 361 GACGAGTGCA AGGGAATTAT TTGAATCACA TAGGAGTGGG AAGTAGACGT GGAGACCTGA 421 CAATTGTAGC TGCAAGTCCT CCAACAGAGG ATGCTGTTGT TGCAGCAGAG CCGTTAACAA 481 AAGAAGATCT TGTAGGATAT CTTGCTTCTG GATGCAAATC CAAANAAAAG TGGAGGATAG 541 GCACTGAACA TGAAAAGTTT GGTTTCGAGT TTGGAACCCT GCGACCCATG AAGTATGATC 601 AAATAGCTGA CTTGCTAAAT GGTATTGCTG AGCGGTTTGA TTGGGAAAAA GTATGGGAGG 661 GTGACAAGAT TATTGGCCTG AAACAGGGGA AAGCAAGCAT ATCA Predicted gene structure (within gDNA segment 86436 to 82741): Exon 1 85836 85640 ( 197 n); cDNA 1 197 ( 197 n); score: 1.000 Intron 1 85639 84713 ( 927 n); Pd: 0.935 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 2 84712 84375 ( 338 n); cDNA 198 535 ( 338 n); score: 0.997 Intron 2 84374 83673 ( 702 n); Pd: 0.992 (s: 0.98), Pa: 0.746 (s: 1.00) Exon 3 83672 83522 ( 151 n); cDNA 536 686 ( 151 n); score: 0.987 Intron 3 83521 83329 ( 193 n); Pd: 0.994 (s: 0.96), Pa: 0.994 (s: 0) Exon 4 83328 83311 ( 18 n); cDNA 687 704 ( 18 n); score: 0.778 MATCH C08HBa0251H04.1- SGN-E348164+ 0.996 704 1.000 C PGS_C08HBa0251H04.1-_SGN-E348164+ (85836 85640,84712 84375,83672 83522,83328 83311) Alignment (genomic DNA sequence = upper lines): TCTCTCTTTA TAGCTAAACA AAAATTAAAG AAATGGGAGT GATAAAGCTC TAGCCGCCTC 85777 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCTCTTTA TAGCTAAACA AAAATTAAAG AAATGGGAGT GATAAAGCTC TAGCCGCCTC 60 AGCACACCAA AATCCTAATC ATTTTGCTGA TACCCAATTG TCTCTATATA TATATTGGAA 85717 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCACACCAA AATCCTAATC ATTTTGCTGA TACCCAATTG TCTCTATATA TATATTGGAA 120 GGTTTTCTTT CAATTTTCAG TCAATTCTAT AGAACAAACC CTACAAAGTC TTAATTTTTC 85657 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTTTTCTTT CAATTTTCAG TCAATTCTAT AGAACAAACC CTACAAAGTC TTAATTTTTC 180 CAGTTTACTG AGCCAAGGTA TCACCTTTTT GTCACCTTTT ATGTTGGTTT CAGTTAGTTT 85597 |||||||||| ||||||| CAGTTTACTG AGCCAAG... .......... .......... .......... .......... 197 TGTTAATCAA GATTCAATTT TTCTGACATA CCCATGTTTC TTTTGTATGC TTTTGTTGCA 85537 .......... .......... .......... .......... .......... .......... 197 TTTCTGTTTT CTTCATGAAG TTGTTTTGGT TAATCAAGAT TCAGTTTTTC TGAAGCCCCA 85477 .......... .......... .......... .......... .......... .......... 197 TGATTTTTTT CTTTTAGTTT TGGTTTGATG GTTTTGTTAG TTGTGTTGTA ATTCTTGTTT 85417 .......... .......... .......... .......... .......... .......... 197 GTTGCCATAT GGGGGTTGAT AGTATTGATT AAAGATTCAA AATTTATACA TGTGGGTTGT 85357 .......... .......... .......... .......... .......... .......... 197 GGTAGTCTGA TTGAATGACA TGCATAGTAA ACTAGAAAGA AGAAAGAGAT AAATGCTTTA 85297 .......... .......... .......... .......... .......... .......... 197 GATAATGTGA ACAAGACTTG TATTATAGGT TAGACTGCTT TACAAGTTTC AGTTTCTCGT 85237 .......... .......... .......... .......... .......... .......... 197 GTTCTTCTGA GTAGTTTTAA GTGTTGAATG AAATGGCATT GTGTAAGATT GCATTTGATG 85177 .......... .......... .......... .......... .......... .......... 197 GTCTGGGATT CAGGTATGAT TGATGCAGGA CAGGAATATC AAGCCACATT TTCTTTATTG 85117 .......... .......... .......... .......... .......... .......... 197 TAGCTGTGTT TCGATATGGA ATGAGATGGG GGTGGGGTGG GGGAGTTTAC AGCATGTAGA 85057 .......... .......... .......... .......... .......... .......... 197 ATTGTAGAGC ACTGTAATGA AAATTTCTAG ATTATTGCAA TTCGAATCTT ATGTTGCTTA 84997 .......... .......... .......... .......... .......... .......... 197 TATAGTACAT CTTAATTAAC TTTAATATTT GTTCGGATTG GTGATTGAGC CTGCTGGGTA 84937 .......... .......... .......... .......... .......... .......... 197 GTTGGGTTGG CCCTATCCTA TTGGTGTATA ACTATGCTCA ATGAAGATGC GAGAGGTTCA 84877 .......... .......... .......... .......... .......... .......... 197 TTTGACGTTT CCCATCAAAA GTACTCTTCA ATCATTATTT AGTTATCCTC TTCTGTTACT 84817 .......... .......... .......... .......... .......... .......... 197 AAATGTAATA CACAAGTTAA ACAATAATCT GAATTCCTTG TCTACTCAAA TGGCATACAT 84757 .......... .......... .......... .......... .......... .......... 197 TCTAGTTAAA AGTGGTTGAC ATCAGTTTTT CCTGTGATAT TCAGGCACAA AGCATGGCCT 84697 |||||| |||||||||| .......... .......... .......... .......... ....GCACAA AGCATGGCCT 213 TGATGTCTCA GGCAGGTTCT TCACATTGCA TTTACTCTGA AAAGGTAAGA TGTATCAGTG 84637 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATGTCTCA GGCAGGTTCT TCACATTGCA TTTACTCTGA AAAGGTAAGA TGTATCAGTG 273 GACATAGAAG CATCATTAAC AATATGGATA TGTTCAGAAT GCGGGAGATA TGCTTTGGCG 84577 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GACATAGAAG CATCATTAAC AATATGGATA TGTTCAGAAT GCGGGAGATA TGCTTTGGCG 333 TGGATATTTC TTCACGCAAT GCCTCAAGAC GAGTGCAAGG GAATTATTTG AATCACATAG 84517 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGATATTTC TTCACGCAAT GCCTCAAGAC GAGTGCAAGG GAATTATTTG AATCACATAG 393 GAGTGGGAAG TAGACGTGGA GACCTGACAA TTGTAGCTGC AAGTCCTCCA ACAGAGGATG 84457 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGTGGGAAG TAGACGTGGA GACCTGACAA TTGTAGCTGC AAGTCCTCCA ACAGAGGATG 453 CTGTTGTTGC AGCAGAGCCG TTAACAAAAG AAGATCTTGT AGGATATCTT GCTTCTGGAT 84397 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTGTTGC AGCAGAGCCG TTAACAAAAG AAGATCTTGT AGGATATCTT GCTTCTGGAT 513 GCAAATCCAA AGAAAAGTGG AGGTTCGAAG TTAAACTTTT CTTAGTGCTC TTAAATTTCA 84337 |||||||||| | |||||||| || GCAAATCCAA ANAAAAGTGG AG........ .......... .......... .......... 535 GTTGCATATA GTTAACCAAG AATATGAATA TTCATTCTCT CGTTTGGCCT TTATAAGTAG 84277 .......... .......... .......... .......... .......... .......... 535 AATAGTCTGC TCATTCACCA CATATATGCC TTGATTGTCT TTCAAGTATG TCGAGTAATT 84217 .......... .......... .......... .......... .......... .......... 535 CATGAATCAA TTCACATCGC TTGTGTATTT TAATATACGG CTATTTTTTA TGTAGTTATT 84157 .......... .......... .......... .......... .......... .......... 535 CTTTCTTCAG CTTCTGTTGT AGTGTATGAT ACGGTAGATG TATTATATAC TTTTTACATG 84097 .......... .......... .......... .......... .......... .......... 535 TCATTATTGT GGTTATTTCT CATTTTCCAT TTCTAAGAAA TATAAATATC ATGGGCAAAT 84037 .......... .......... .......... .......... .......... .......... 535 TCTCACCGTC ATGCATTCAA GATGTGGATA CTAGGGAACT AGGACATGAT GCAGCTCTTT 83977 .......... .......... .......... .......... .......... .......... 535 TCATTGAACT AACAAGTTGT ACCATTTGGG CAACTTGAAC ACTGGAAACA TTAATCAATT 83917 .......... .......... .......... .......... .......... .......... 535 TTTCCTAGGA CACTGCTGTC TCTAATTTTG TTGCAAATTT TGAATTGTTG AAATTTCGGT 83857 .......... .......... .......... .......... .......... .......... 535 AATACAATTT TTGAACATGG AGTTGGGAGC CATTCCCTGG ATCAGGTATT TTTTCATAAA 83797 .......... .......... .......... .......... .......... .......... 535 TGTGGTAGCT AGGATTCTGC CTCAGTCATT ATTTGGATAG TTTGTTTTAC TTAACTGTGC 83737 .......... .......... .......... .......... .......... .......... 535 GTCTGGATCT AGACCATAAT AATCAGGTTC AACTTTTCTT GTTCTCTCAA AACTTGATGA 83677 .......... .......... .......... .......... .......... .......... 535 GCAGGATAGG CACTGAACAT GAAAAGTTTG GTTTCGAGTT TGGAACCCTG CGACCCATGA 83617 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....GATAGG CACTGAACAT GAAAAGTTTG GTTTCGAGTT TGGAACCCTG CGACCCATGA 591 AGTATGATCA AATAGCTGAC TTGCTAAATG GTATTGCTGA GCGGTTTGAT TGGGAAAAAG 83557 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTATGATCA AATAGCTGAC TTGCTAAATG GTATTGCTGA GCGGTTTGAT TGGGAAAAAG 651 TAATGGAGGG TGACAAGATT ATTGGCCTGA AACAGGTAAC GTGAACTTGG TGCAAACTTT 83497 || |||||| |||||||||| |||||||||| ||||| TATGGGAGGG TGACAAGATT ATTGGCCTGA AACAG..... .......... .......... 686 GGTTTAAAAT ATTCTAGGTT GAATTTGTCA TGGCTCCATA CAATGTATTA TGCTACTTAC 83437 .......... .......... .......... .......... .......... .......... 686 CATGAATAGA ATCATAATTC CTGGTTCATT TTTTTCCTCA AGTGCAAGTC TGTAGTAAGT 83377 .......... .......... .......... .......... .......... .......... 686 AGTAATTACT TTAGCATAAT TGGTGGATAA TTTGCACTGT CTTCGCAGGG AAAGCAAAGC 83317 || || |||| .......... .......... .......... .......... ........GG GAAAGCAAGC 698 ATATCA 83311 |||||| ATATCA 704 hqPGS_C08HBa0251H04.1-_SGN-E348164+ (85836 85640,84712 84375,83672 83522,83328 83311) ******************************************************************************** EST sequence 98 +strand 647 n (File: SGN-E342804+) 1 TTGTCTCTCT TTATAGCTAA ACAAAAATTA AAGAAATGGG AGTGATAAAG CTCTAGCCGC 61 CTCAGCACAC CAAAATCCTA ATCATTTTGC TGATACCCAA TTGTCTCTAT ATATATATTG 121 GAAGGTTTTC TTTCAATTTT CAGTCAATTC TATAGAACAA ACCCTACAAA GTCTTAATTT 181 TTCCAGTTTA CTGAGCCAAG GCACAAAGCA TGGCCTTGAT GTCTCAGGCA GGTTCTTCAC 241 ATTGCATTTA CTCTGAAAAG GTAAGATGTA TCAGTGGACA TAGAAGCATC ATTAACAATA 301 TGGATATGTT CAGAATGCGG GAGATATGCT TTGGCGTGGA TATTTCTTCA CGCAATGCCT 361 CAAGACGAGT GCAAGGGAAT TATTTGAATC ACATAGGAGT GGGAAGTAGA CGTGGAGACC 421 TGACAATTGT AGCTGCAAGT CCTCCAACAG AGGATGCTGT TGTTGCAGCA GAGCCGTTAC 481 AAAAGAAGAT CTTGTAGGAT ATCTTGCTTC TGGATGCAAA TCCAAAGAAA AGTGGAGGAT 541 AGGCACTGAA CATGAAAAGT TTGGTTTCGA GTTTGGAACC CTGCGACCCA TGAAGTATGA 601 TCAAATAGCT GACTTGCTAA ATGGTATTGC TGAGCGGTTT GATTGGG Predicted gene structure (within gDNA segment 86439 to 82953): Exon 1 85839 85640 ( 200 n); cDNA 1 200 ( 200 n); score: 1.000 Intron 1 85639 84713 ( 927 n); Pd: 0.935 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 2 84712 84375 ( 338 n); cDNA 201 537 ( 337 n); score: 0.997 Intron 2 84374 83673 ( 702 n); Pd: 0.992 (s: 1.00), Pa: 0.746 (s: 1.00) Exon 3 83672 83563 ( 110 n); cDNA 538 647 ( 110 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E342804+ 0.998 648 1.002 C PGS_C08HBa0251H04.1-_SGN-E342804+ (85839 85640,84712 84375,83672 83563) Alignment (genomic DNA sequence = upper lines): TTGTCTCTCT TTATAGCTAA ACAAAAATTA AAGAAATGGG AGTGATAAAG CTCTAGCCGC 85780 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTCTCTCT TTATAGCTAA ACAAAAATTA AAGAAATGGG AGTGATAAAG CTCTAGCCGC 60 CTCAGCACAC CAAAATCCTA ATCATTTTGC TGATACCCAA TTGTCTCTAT ATATATATTG 85720 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCAGCACAC CAAAATCCTA ATCATTTTGC TGATACCCAA TTGTCTCTAT ATATATATTG 120 GAAGGTTTTC TTTCAATTTT CAGTCAATTC TATAGAACAA ACCCTACAAA GTCTTAATTT 85660 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGGTTTTC TTTCAATTTT CAGTCAATTC TATAGAACAA ACCCTACAAA GTCTTAATTT 180 TTCCAGTTTA CTGAGCCAAG GTATCACCTT TTTGTCACCT TTTATGTTGG TTTCAGTTAG 85600 |||||||||| |||||||||| TTCCAGTTTA CTGAGCCAAG .......... .......... .......... .......... 200 TTTTGTTAAT CAAGATTCAA TTTTTCTGAC ATACCCATGT TTCTTTTGTA TGCTTTTGTT 85540 .......... .......... .......... .......... .......... .......... 200 GCATTTCTGT TTTCTTCATG AAGTTGTTTT GGTTAATCAA GATTCAGTTT TTCTGAAGCC 85480 .......... .......... .......... .......... .......... .......... 200 CCATGATTTT TTTCTTTTAG TTTTGGTTTG ATGGTTTTGT TAGTTGTGTT GTAATTCTTG 85420 .......... .......... .......... .......... .......... .......... 200 TTTGTTGCCA TATGGGGGTT GATAGTATTG ATTAAAGATT CAAAATTTAT ACATGTGGGT 85360 .......... .......... .......... .......... .......... .......... 200 TGTGGTAGTC TGATTGAATG ACATGCATAG TAAACTAGAA AGAAGAAAGA GATAAATGCT 85300 .......... .......... .......... .......... .......... .......... 200 TTAGATAATG TGAACAAGAC TTGTATTATA GGTTAGACTG CTTTACAAGT TTCAGTTTCT 85240 .......... .......... .......... .......... .......... .......... 200 CGTGTTCTTC TGAGTAGTTT TAAGTGTTGA ATGAAATGGC ATTGTGTAAG ATTGCATTTG 85180 .......... .......... .......... .......... .......... .......... 200 ATGGTCTGGG ATTCAGGTAT GATTGATGCA GGACAGGAAT ATCAAGCCAC ATTTTCTTTA 85120 .......... .......... .......... .......... .......... .......... 200 TTGTAGCTGT GTTTCGATAT GGAATGAGAT GGGGGTGGGG TGGGGGAGTT TACAGCATGT 85060 .......... .......... .......... .......... .......... .......... 200 AGAATTGTAG AGCACTGTAA TGAAAATTTC TAGATTATTG CAATTCGAAT CTTATGTTGC 85000 .......... .......... .......... .......... .......... .......... 200 TTATATAGTA CATCTTAATT AACTTTAATA TTTGTTCGGA TTGGTGATTG AGCCTGCTGG 84940 .......... .......... .......... .......... .......... .......... 200 GTAGTTGGGT TGGCCCTATC CTATTGGTGT ATAACTATGC TCAATGAAGA TGCGAGAGGT 84880 .......... .......... .......... .......... .......... .......... 200 TCATTTGACG TTTCCCATCA AAAGTACTCT TCAATCATTA TTTAGTTATC CTCTTCTGTT 84820 .......... .......... .......... .......... .......... .......... 200 ACTAAATGTA ATACACAAGT TAAACAATAA TCTGAATTCC TTGTCTACTC AAATGGCATA 84760 .......... .......... .......... .......... .......... .......... 200 CATTCTAGTT AAAAGTGGTT GACATCAGTT TTTCCTGTGA TATTCAGGCA CAAAGCATGG 84700 ||| |||||||||| .......... .......... .......... .......... .......GCA CAAAGCATGG 213 CCTTGATGTC TCAGGCAGGT TCTTCACATT GCATTTACTC TGAAAAGGTA AGATGTATCA 84640 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCTTGATGTC TCAGGCAGGT TCTTCACATT GCATTTACTC TGAAAAGGTA AGATGTATCA 273 GTGGACATAG AAGCATCATT AACAATATGG ATATGTTCAG AATGCGGGAG ATATGCTTTG 84580 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGGACATAG AAGCATCATT AACAATATGG ATATGTTCAG AATGCGGGAG ATATGCTTTG 333 GCGTGGATAT TTCTTCACGC AATGCCTCAA GACGAGTGCA AGGGAATTAT TTGAATCACA 84520 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCGTGGATAT TTCTTCACGC AATGCCTCAA GACGAGTGCA AGGGAATTAT TTGAATCACA 393 TAGGAGTGGG AAGTAGACGT GGAGACCTGA CAATTGTAGC TGCAAGTCCT CCAACAGAGG 84460 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGGAGTGGG AAGTAGACGT GGAGACCTGA CAATTGTAGC TGCAAGTCCT CCAACAGAGG 453 ATGCTGTTGT TGCAGCAGAG CCGTTAACAA AAGAAGATCT TGTAGGATAT CTTGCTTCTG 84400 |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| ATGCTGTTGT TGCAGCAGAG CCGTT-ACAA AAGAAGATCT TGTAGGATAT CTTGCTTCTG 512 GATGCAAATC CAAAGAAAAG TGGAGGTTCG AAGTTAAACT TTTCTTAGTG CTCTTAAATT 84340 |||||||||| |||||||||| ||||| GATGCAAATC CAAAGAAAAG TGGAG..... .......... .......... .......... 537 TCAGTTGCAT ATAGTTAACC AAGAATATGA ATATTCATTC TCTCGTTTGG CCTTTATAAG 84280 .......... .......... .......... .......... .......... .......... 537 TAGAATAGTC TGCTCATTCA CCACATATAT GCCTTGATTG TCTTTCAAGT ATGTCGAGTA 84220 .......... .......... .......... .......... .......... .......... 537 ATTCATGAAT CAATTCACAT CGCTTGTGTA TTTTAATATA CGGCTATTTT TTATGTAGTT 84160 .......... .......... .......... .......... .......... .......... 537 ATTCTTTCTT CAGCTTCTGT TGTAGTGTAT GATACGGTAG ATGTATTATA TACTTTTTAC 84100 .......... .......... .......... .......... .......... .......... 537 ATGTCATTAT TGTGGTTATT TCTCATTTTC CATTTCTAAG AAATATAAAT ATCATGGGCA 84040 .......... .......... .......... .......... .......... .......... 537 AATTCTCACC GTCATGCATT CAAGATGTGG ATACTAGGGA ACTAGGACAT GATGCAGCTC 83980 .......... .......... .......... .......... .......... .......... 537 TTTTCATTGA ACTAACAAGT TGTACCATTT GGGCAACTTG AACACTGGAA ACATTAATCA 83920 .......... .......... .......... .......... .......... .......... 537 ATTTTTCCTA GGACACTGCT GTCTCTAATT TTGTTGCAAA TTTTGAATTG TTGAAATTTC 83860 .......... .......... .......... .......... .......... .......... 537 GGTAATACAA TTTTTGAACA TGGAGTTGGG AGCCATTCCC TGGATCAGGT ATTTTTTCAT 83800 .......... .......... .......... .......... .......... .......... 537 AAATGTGGTA GCTAGGATTC TGCCTCAGTC ATTATTTGGA TAGTTTGTTT TACTTAACTG 83740 .......... .......... .......... .......... .......... .......... 537 TGCGTCTGGA TCTAGACCAT AATAATCAGG TTCAACTTTT CTTGTTCTCT CAAAACTTGA 83680 .......... .......... .......... .......... .......... .......... 537 TGAGCAGGAT AGGCACTGAA CATGAAAAGT TTGGTTTCGA GTTTGGAACC CTGCGACCCA 83620 ||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......GAT AGGCACTGAA CATGAAAAGT TTGGTTTCGA GTTTGGAACC CTGCGACCCA 590 TGAAGTATGA TCAAATAGCT GACTTGCTAA ATGGTATTGC TGAGCGGTTT GATTGGG 83563 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TGAAGTATGA TCAAATAGCT GACTTGCTAA ATGGTATTGC TGAGCGGTTT GATTGGG 647 hqPGS_C08HBa0251H04.1-_SGN-E342804+ (85839 85640,84712 84375,83672 83563) ******************************************************************************** EST sequence 99 +strand 465 n (File: SGN-E306778+) 1 AAGTCTTAAT TTTTCCAGTT TACTGAGCCA AAGCACAAAG CATGGCCTTG ATGTCTCAGG 61 CAGGTTCTTC ACATTGCATT TACTCTGAAA AGGTAAGATG TATCAGTGGA CATAGAAGCA 121 TCATTAACAA TATGGATATG TTCAGAATGC GGGAGATATG CTTTGGCGTG GATATTTCTT 181 CACGCAATGC CTCAAGACGA GTGCAAGGGA ATTATTTGAA TCACATAGGA GTGGGAAGTA 241 GACGTGGAGA CCTGACAATT GTAGCTGCAA GTCCTCCAAC AGAGGATGCT GTTGTTGCAG 301 CAGAGCCGTT AACAAAAGAA GATCTTGTAG GATATCTTGC TTCTGGATGC AAATCCAAAG 361 AAAAGTGGAG GATAGGCACT GAACATGAAA AGTTTGGTTT CGAGTTTGGA ACCCTGCGAC 421 CCATGAAGTA TGATCAAATA GCTGACTTGC TAAATGGTAT TGCTG Predicted gene structure (within gDNA segment 86271 to 82968): Exon 1 85671 85640 ( 32 n); cDNA 1 32 ( 32 n); score: 0.969 Intron 1 85639 84713 ( 927 n); Pd: 0.935 (s: 0), Pa: 0.993 (s: 1.00) Exon 2 84712 84375 ( 338 n); cDNA 33 370 ( 338 n); score: 1.000 Intron 2 84374 83673 ( 702 n); Pd: 0.992 (s: 1.00), Pa: 0.746 (s: 1.00) Exon 3 83672 83578 ( 95 n); cDNA 371 465 ( 95 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E306778+ 1.000 465 1.000 C PGS_C08HBa0251H04.1-_SGN-E306778+ (85671 85640,84712 84375,83672 83578) Alignment (genomic DNA sequence = upper lines): AAGTCTTAAT TTTTCCAGTT TACTGAGCCA AGGTATCACC TTTTTGTCAC CTTTTATGTT 85612 |||||||||| |||||||||| |||||||||| | AAGTCTTAAT TTTTCCAGTT TACTGAGCCA AA........ .......... .......... 32 GGTTTCAGTT AGTTTTGTTA ATCAAGATTC AATTTTTCTG ACATACCCAT GTTTCTTTTG 85552 .......... .......... .......... .......... .......... .......... 32 TATGCTTTTG TTGCATTTCT GTTTTCTTCA TGAAGTTGTT TTGGTTAATC AAGATTCAGT 85492 .......... .......... .......... .......... .......... .......... 32 TTTTCTGAAG CCCCATGATT TTTTTCTTTT AGTTTTGGTT TGATGGTTTT GTTAGTTGTG 85432 .......... .......... .......... .......... .......... .......... 32 TTGTAATTCT TGTTTGTTGC CATATGGGGG TTGATAGTAT TGATTAAAGA TTCAAAATTT 85372 .......... .......... .......... .......... .......... .......... 32 ATACATGTGG GTTGTGGTAG TCTGATTGAA TGACATGCAT AGTAAACTAG AAAGAAGAAA 85312 .......... .......... .......... .......... .......... .......... 32 GAGATAAATG CTTTAGATAA TGTGAACAAG ACTTGTATTA TAGGTTAGAC TGCTTTACAA 85252 .......... .......... .......... .......... .......... .......... 32 GTTTCAGTTT CTCGTGTTCT TCTGAGTAGT TTTAAGTGTT GAATGAAATG GCATTGTGTA 85192 .......... .......... .......... .......... .......... .......... 32 AGATTGCATT TGATGGTCTG GGATTCAGGT ATGATTGATG CAGGACAGGA ATATCAAGCC 85132 .......... .......... .......... .......... .......... .......... 32 ACATTTTCTT TATTGTAGCT GTGTTTCGAT ATGGAATGAG ATGGGGGTGG GGTGGGGGAG 85072 .......... .......... .......... .......... .......... .......... 32 TTTACAGCAT GTAGAATTGT AGAGCACTGT AATGAAAATT TCTAGATTAT TGCAATTCGA 85012 .......... .......... .......... .......... .......... .......... 32 ATCTTATGTT GCTTATATAG TACATCTTAA TTAACTTTAA TATTTGTTCG GATTGGTGAT 84952 .......... .......... .......... .......... .......... .......... 32 TGAGCCTGCT GGGTAGTTGG GTTGGCCCTA TCCTATTGGT GTATAACTAT GCTCAATGAA 84892 .......... .......... .......... .......... .......... .......... 32 GATGCGAGAG GTTCATTTGA CGTTTCCCAT CAAAAGTACT CTTCAATCAT TATTTAGTTA 84832 .......... .......... .......... .......... .......... .......... 32 TCCTCTTCTG TTACTAAATG TAATACACAA GTTAAACAAT AATCTGAATT CCTTGTCTAC 84772 .......... .......... .......... .......... .......... .......... 32 TCAAATGGCA TACATTCTAG TTAAAAGTGG TTGACATCAG TTTTTCCTGT GATATTCAGG 84712 | .......... .......... .......... .......... .......... .........G 33 CACAAAGCAT GGCCTTGATG TCTCAGGCAG GTTCTTCACA TTGCATTTAC TCTGAAAAGG 84652 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACAAAGCAT GGCCTTGATG TCTCAGGCAG GTTCTTCACA TTGCATTTAC TCTGAAAAGG 93 TAAGATGTAT CAGTGGACAT AGAAGCATCA TTAACAATAT GGATATGTTC AGAATGCGGG 84592 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAGATGTAT CAGTGGACAT AGAAGCATCA TTAACAATAT GGATATGTTC AGAATGCGGG 153 AGATATGCTT TGGCGTGGAT ATTTCTTCAC GCAATGCCTC AAGACGAGTG CAAGGGAATT 84532 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATATGCTT TGGCGTGGAT ATTTCTTCAC GCAATGCCTC AAGACGAGTG CAAGGGAATT 213 ATTTGAATCA CATAGGAGTG GGAAGTAGAC GTGGAGACCT GACAATTGTA GCTGCAAGTC 84472 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTGAATCA CATAGGAGTG GGAAGTAGAC GTGGAGACCT GACAATTGTA GCTGCAAGTC 273 CTCCAACAGA GGATGCTGTT GTTGCAGCAG AGCCGTTAAC AAAAGAAGAT CTTGTAGGAT 84412 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCCAACAGA GGATGCTGTT GTTGCAGCAG AGCCGTTAAC AAAAGAAGAT CTTGTAGGAT 333 ATCTTGCTTC TGGATGCAAA TCCAAAGAAA AGTGGAGGTT CGAAGTTAAA CTTTTCTTAG 84352 |||||||||| |||||||||| |||||||||| ||||||| ATCTTGCTTC TGGATGCAAA TCCAAAGAAA AGTGGAG... .......... .......... 370 TGCTCTTAAA TTTCAGTTGC ATATAGTTAA CCAAGAATAT GAATATTCAT TCTCTCGTTT 84292 .......... .......... .......... .......... .......... .......... 370 GGCCTTTATA AGTAGAATAG TCTGCTCATT CACCACATAT ATGCCTTGAT TGTCTTTCAA 84232 .......... .......... .......... .......... .......... .......... 370 GTATGTCGAG TAATTCATGA ATCAATTCAC ATCGCTTGTG TATTTTAATA TACGGCTATT 84172 .......... .......... .......... .......... .......... .......... 370 TTTTATGTAG TTATTCTTTC TTCAGCTTCT GTTGTAGTGT ATGATACGGT AGATGTATTA 84112 .......... .......... .......... .......... .......... .......... 370 TATACTTTTT ACATGTCATT ATTGTGGTTA TTTCTCATTT TCCATTTCTA AGAAATATAA 84052 .......... .......... .......... .......... .......... .......... 370 ATATCATGGG CAAATTCTCA CCGTCATGCA TTCAAGATGT GGATACTAGG GAACTAGGAC 83992 .......... .......... .......... .......... .......... .......... 370 ATGATGCAGC TCTTTTCATT GAACTAACAA GTTGTACCAT TTGGGCAACT TGAACACTGG 83932 .......... .......... .......... .......... .......... .......... 370 AAACATTAAT CAATTTTTCC TAGGACACTG CTGTCTCTAA TTTTGTTGCA AATTTTGAAT 83872 .......... .......... .......... .......... .......... .......... 370 TGTTGAAATT TCGGTAATAC AATTTTTGAA CATGGAGTTG GGAGCCATTC CCTGGATCAG 83812 .......... .......... .......... .......... .......... .......... 370 GTATTTTTTC ATAAATGTGG TAGCTAGGAT TCTGCCTCAG TCATTATTTG GATAGTTTGT 83752 .......... .......... .......... .......... .......... .......... 370 TTTACTTAAC TGTGCGTCTG GATCTAGACC ATAATAATCA GGTTCAACTT TTCTTGTTCT 83692 .......... .......... .......... .......... .......... .......... 370 CTCAAAACTT GATGAGCAGG ATAGGCACTG AACATGAAAA GTTTGGTTTC GAGTTTGGAA 83632 | |||||||||| |||||||||| |||||||||| |||||||||| .......... .........G ATAGGCACTG AACATGAAAA GTTTGGTTTC GAGTTTGGAA 411 CCCTGCGACC CATGAAGTAT GATCAAATAG CTGACTTGCT AAATGGTATT GCTG 83578 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| CCCTGCGACC CATGAAGTAT GATCAAATAG CTGACTTGCT AAATGGTATT GCTG 465 hqPGS_C08HBa0251H04.1-_SGN-E306778+ (85671 85640,84712 84375,83672 83578) ******************************************************************************** EST sequence 121 +strand 535 n (File: SGN-E207723+) 1 ACACCAAAAT CCTAATCATT TTGCTGATAC CCAATTGTCT CTATATATAT ATTGGAAGGT 61 TTTCTTTCAA TTTTCAGTCA ATTCTATAGA ACAAACCCTA CAAAGTCTTA ATTTTTCCAG 121 TTTACTGAGC CAAGGCACAA AGCATGGCCT TGATGTCTCA GGCAGGTTCT TCACATTGCA 181 TTTACTCTGA AAAGGTAAGA TGTATCAGTG GACATAGAAG CATCATTAAC AATATGGATA 241 TGTTCAGAAT GCGGGAGATA TGCTTTGGCG TGGATATTTC TTCACGCAAT GCCTCAAGAC 301 GAGTGCAAGG GAATTATTTG AATCACATAG GAGTGGGAAG TAGACGTGGA GACCTGACAA 361 TTGTAGCTGC AAGTCCTCCA ACAGAGGATG CTGTTGTTGC AGCAGAGCCG TTAACAAAAG 421 AAGATCTTGT AGGATATCTT GCTTCTGGAT GCAAATCCAA AGAAAAGTGG AGGATAGGCA 481 CTGAACATGA AAAGTTTGGT TTCGAGTTTG GAACCCTGCG ACCCATGAAG TATGA Predicted gene structure (within gDNA segment 86373 to 83000): Exon 1 85773 85640 ( 134 n); cDNA 1 134 ( 134 n); score: 1.000 Intron 1 85639 84713 ( 927 n); Pd: 0.935 (s: 1.00), Pa: 0.993 (s: 1.00) Exon 2 84712 84375 ( 338 n); cDNA 135 472 ( 338 n); score: 1.000 Intron 2 84374 83673 ( 702 n); Pd: 0.992 (s: 1.00), Pa: 0.746 (s: 1.00) Exon 3 83672 83610 ( 63 n); cDNA 473 535 ( 63 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E207723+ 1.000 535 1.000 C PGS_C08HBa0251H04.1-_SGN-E207723+ (85773 85640,84712 84375,83672 83610) Alignment (genomic DNA sequence = upper lines): ACACCAAAAT CCTAATCATT TTGCTGATAC CCAATTGTCT CTATATATAT ATTGGAAGGT 85714 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACACCAAAAT CCTAATCATT TTGCTGATAC CCAATTGTCT CTATATATAT ATTGGAAGGT 60 TTTCTTTCAA TTTTCAGTCA ATTCTATAGA ACAAACCCTA CAAAGTCTTA ATTTTTCCAG 85654 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCTTTCAA TTTTCAGTCA ATTCTATAGA ACAAACCCTA CAAAGTCTTA ATTTTTCCAG 120 TTTACTGAGC CAAGGTATCA CCTTTTTGTC ACCTTTTATG TTGGTTTCAG TTAGTTTTGT 85594 |||||||||| |||| TTTACTGAGC CAAG...... .......... .......... .......... .......... 134 TAATCAAGAT TCAATTTTTC TGACATACCC ATGTTTCTTT TGTATGCTTT TGTTGCATTT 85534 .......... .......... .......... .......... .......... .......... 134 CTGTTTTCTT CATGAAGTTG TTTTGGTTAA TCAAGATTCA GTTTTTCTGA AGCCCCATGA 85474 .......... .......... .......... .......... .......... .......... 134 TTTTTTTCTT TTAGTTTTGG TTTGATGGTT TTGTTAGTTG TGTTGTAATT CTTGTTTGTT 85414 .......... .......... .......... .......... .......... .......... 134 GCCATATGGG GGTTGATAGT ATTGATTAAA GATTCAAAAT TTATACATGT GGGTTGTGGT 85354 .......... .......... .......... .......... .......... .......... 134 AGTCTGATTG AATGACATGC ATAGTAAACT AGAAAGAAGA AAGAGATAAA TGCTTTAGAT 85294 .......... .......... .......... .......... .......... .......... 134 AATGTGAACA AGACTTGTAT TATAGGTTAG ACTGCTTTAC AAGTTTCAGT TTCTCGTGTT 85234 .......... .......... .......... .......... .......... .......... 134 CTTCTGAGTA GTTTTAAGTG TTGAATGAAA TGGCATTGTG TAAGATTGCA TTTGATGGTC 85174 .......... .......... .......... .......... .......... .......... 134 TGGGATTCAG GTATGATTGA TGCAGGACAG GAATATCAAG CCACATTTTC TTTATTGTAG 85114 .......... .......... .......... .......... .......... .......... 134 CTGTGTTTCG ATATGGAATG AGATGGGGGT GGGGTGGGGG AGTTTACAGC ATGTAGAATT 85054 .......... .......... .......... .......... .......... .......... 134 GTAGAGCACT GTAATGAAAA TTTCTAGATT ATTGCAATTC GAATCTTATG TTGCTTATAT 84994 .......... .......... .......... .......... .......... .......... 134 AGTACATCTT AATTAACTTT AATATTTGTT CGGATTGGTG ATTGAGCCTG CTGGGTAGTT 84934 .......... .......... .......... .......... .......... .......... 134 GGGTTGGCCC TATCCTATTG GTGTATAACT ATGCTCAATG AAGATGCGAG AGGTTCATTT 84874 .......... .......... .......... .......... .......... .......... 134 GACGTTTCCC ATCAAAAGTA CTCTTCAATC ATTATTTAGT TATCCTCTTC TGTTACTAAA 84814 .......... .......... .......... .......... .......... .......... 134 TGTAATACAC AAGTTAAACA ATAATCTGAA TTCCTTGTCT ACTCAAATGG CATACATTCT 84754 .......... .......... .......... .......... .......... .......... 134 AGTTAAAAGT GGTTGACATC AGTTTTTCCT GTGATATTCA GGCACAAAGC ATGGCCTTGA 84694 ||||||||| |||||||||| .......... .......... .......... .......... .GCACAAAGC ATGGCCTTGA 153 TGTCTCAGGC AGGTTCTTCA CATTGCATTT ACTCTGAAAA GGTAAGATGT ATCAGTGGAC 84634 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTCTCAGGC AGGTTCTTCA CATTGCATTT ACTCTGAAAA GGTAAGATGT ATCAGTGGAC 213 ATAGAAGCAT CATTAACAAT ATGGATATGT TCAGAATGCG GGAGATATGC TTTGGCGTGG 84574 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATAGAAGCAT CATTAACAAT ATGGATATGT TCAGAATGCG GGAGATATGC TTTGGCGTGG 273 ATATTTCTTC ACGCAATGCC TCAAGACGAG TGCAAGGGAA TTATTTGAAT CACATAGGAG 84514 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATATTTCTTC ACGCAATGCC TCAAGACGAG TGCAAGGGAA TTATTTGAAT CACATAGGAG 333 TGGGAAGTAG ACGTGGAGAC CTGACAATTG TAGCTGCAAG TCCTCCAACA GAGGATGCTG 84454 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGGAAGTAG ACGTGGAGAC CTGACAATTG TAGCTGCAAG TCCTCCAACA GAGGATGCTG 393 TTGTTGCAGC AGAGCCGTTA ACAAAAGAAG ATCTTGTAGG ATATCTTGCT TCTGGATGCA 84394 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTTGCAGC AGAGCCGTTA ACAAAAGAAG ATCTTGTAGG ATATCTTGCT TCTGGATGCA 453 AATCCAAAGA AAAGTGGAGG TTCGAAGTTA AACTTTTCTT AGTGCTCTTA AATTTCAGTT 84334 |||||||||| ||||||||| AATCCAAAGA AAAGTGGAG. .......... .......... .......... .......... 472 GCATATAGTT AACCAAGAAT ATGAATATTC ATTCTCTCGT TTGGCCTTTA TAAGTAGAAT 84274 .......... .......... .......... .......... .......... .......... 472 AGTCTGCTCA TTCACCACAT ATATGCCTTG ATTGTCTTTC AAGTATGTCG AGTAATTCAT 84214 .......... .......... .......... .......... .......... .......... 472 GAATCAATTC ACATCGCTTG TGTATTTTAA TATACGGCTA TTTTTTATGT AGTTATTCTT 84154 .......... .......... .......... .......... .......... .......... 472 TCTTCAGCTT CTGTTGTAGT GTATGATACG GTAGATGTAT TATATACTTT TTACATGTCA 84094 .......... .......... .......... .......... .......... .......... 472 TTATTGTGGT TATTTCTCAT TTTCCATTTC TAAGAAATAT AAATATCATG GGCAAATTCT 84034 .......... .......... .......... .......... .......... .......... 472 CACCGTCATG CATTCAAGAT GTGGATACTA GGGAACTAGG ACATGATGCA GCTCTTTTCA 83974 .......... .......... .......... .......... .......... .......... 472 TTGAACTAAC AAGTTGTACC ATTTGGGCAA CTTGAACACT GGAAACATTA ATCAATTTTT 83914 .......... .......... .......... .......... .......... .......... 472 CCTAGGACAC TGCTGTCTCT AATTTTGTTG CAAATTTTGA ATTGTTGAAA TTTCGGTAAT 83854 .......... .......... .......... .......... .......... .......... 472 ACAATTTTTG AACATGGAGT TGGGAGCCAT TCCCTGGATC AGGTATTTTT TCATAAATGT 83794 .......... .......... .......... .......... .......... .......... 472 GGTAGCTAGG ATTCTGCCTC AGTCATTATT TGGATAGTTT GTTTTACTTA ACTGTGCGTC 83734 .......... .......... .......... .......... .......... .......... 472 TGGATCTAGA CCATAATAAT CAGGTTCAAC TTTTCTTGTT CTCTCAAAAC TTGATGAGCA 83674 .......... .......... .......... .......... .......... .......... 472 GGATAGGCAC TGAACATGAA AAGTTTGGTT TCGAGTTTGG AACCCTGCGA CCCATGAAGT 83614 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .GATAGGCAC TGAACATGAA AAGTTTGGTT TCGAGTTTGG AACCCTGCGA CCCATGAAGT 531 ATGA 83610 |||| ATGA 535 hqPGS_C08HBa0251H04.1-_SGN-E207723+ (85773 85640,84712 84375,83672 83610) ******************************************************************************** EST sequence 50 -strand 519 n (File: SGN-E311217-) 1 TGTTTGTCTA TGCATCACAG CTTCTACAAT TCATACCAGA GAGGTGTATT GTGTTTGGGA 61 ACTCAAACCA AACCGTCGAG GCTGCACATG ATGCTCAGAT GAAGTGTGTT GCAGTTGCTA 121 GCAAACATCC AGTGTATGAG CTTGGTGCTG CTGACTTGGT CGTGAGGCAC CTTGATGAGC 181 TCTCGATCGT TGACCTAAAG AACCTTGCTG CTGTTGAGCT GACTGAATTT GGATCTCCTG 241 AACCAGAATT GGAAATGGAA GAAGAGGATG ATCCTTATCC TCCATCTGCA GTGGCCGTCG 301 ATGATGGTTT CTGGTAACAT CTCCCCAATG TACAGTAGTC GCGCTTAATA ATATTCAAAT 361 AGGATATAGA AAAATGTAAA TTAAAATGCA GGGCAAGAAG TGTATCAGAA GGTGGTCTAC 421 ATTGGGCTGC CATCAACCTG ACTTTGTATG TGATATTTGT ATTATATATG TATATCTTCT 481 GCCCAAAAAA AAAAAAAAAA AAAACCGAGA CTAGTTCTC Predicted gene structure (within gDNA segment 96890 to 94820): Exon 1 96280 95797 ( 484 n); cDNA 1 484 ( 484 n); score: 0.994 PPA cDNA 485 505 MATCH C08HBa0251H04.1- SGN-E311217- 0.994 484 0.933 C PGS_C08HBa0251H04.1-_SGN-E311217- (96280 95797) Alignment (genomic DNA sequence = upper lines): TGTTTGTCTA TGCATCACAG CTTCTACAAT TCATACCAGA GAGGTGTATT GTGTTTGGGA 96221 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTTGTCTA TGCATCACAG CTTCTACAAT TCATACCAGA GAGGTGTATT GTGTTTGGGA 60 ACTCAAACCA AACCGTCGAG GCTGCACATG ATGCTCAGAT GAAGTGTGTT GCAGTTGCTA 96161 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACTCAAACCA AACCGTCGAG GCTGCACATG ATGCTCAGAT GAAGTGTGTT GCAGTTGCTA 120 GCAAACATCC AGTGTATGAG CTTGGTGCTG CTGACTTGGT TGTGAGGCAC CTTGATGAGC 96101 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| |||||||||| GCAAACATCC AGTGTATGAG CTTGGTGCTG CTGACTTGGT CGTGAGGCAC CTTGATGAGC 180 TCTCGATCGT TGACCTAAAG AACCTTGCTG CTGTTGAGCT GACTGAATTT GGATCTCCTG 96041 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTCGATCGT TGACCTAAAG AACCTTGCTG CTGTTGAGCT GACTGAATTT GGATCTCCTG 240 AACCAGAATT GGAAATGGAA GAAGAGGATG ATCCTTATCC TCCATCTGCA GTGGCCGTCG 95981 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACCAGAATT GGAAATGGAA GAAGAGGATG ATCCTTATCC TCCATCTGCA GTGGCCGTCG 300 ATGATGGTTT CTGGTAACAT CTCCCCAATG TACAGTAGTC GCGCTTAATA ATATTCAAAT 95921 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGATGGTTT CTGGTAACAT CTCCCCAATG TACAGTAGTC GCGCTTAATA ATATTCAAAT 360 AGGATATAGA AAAATGTAAA CTAAAATGCA GGGCAAGAAG TGTATCAGAA GGTGGTCTAC 95861 |||||||||| |||||||||| ||||||||| |||||||||| |||||||||| |||||||||| AGGATATAGA AAAATGTAAA TTAAAATGCA GGGCAAGAAG TGTATCAGAA GGTGGTCTAC 420 ATTGGGCTGC CATCAACCTG ACTTTGTATG TGATATTTGT ATTATATATG TATATCTTCT 95801 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGGGCTGC CATCAACCTG ACTTTGTATG TGATATTTGT ATTATATATG TATATCTTCT 480 GACC 95797 | || GCCC 484 hqPGS_C08HBa0251H04.1-_SGN-E311217- (96280 95797) ******************************************************************************** EST sequence 47 -strand 317 n (File: SGN-E309792-) 1 CTTGCTGCTG TTGAGCTGAC TGAATTTGGA TCTCCTGAAC CAGAATTGGA AATGGAAGAA 61 GAGGATGATC CTTATCCTCC ATCTGCAGTG GCCGTCGATG ATGGTTTCTG GTAACATCTC 121 CCCAATGTAC AGTAGTCGCG CTTAATAATA TTCAAATAGG ATATAGAAAA ATGTAAACTA 181 AAATGCAGGG CAAGAAGTGT ATCAGAAGGT GGTCTACATT GGGCTGCCAT CAACCTGACT 241 TTGTATGTGA TATTTGTATT ATATATGTAT ATCTTCTGAC CAAAAAAAAA AAAAAAAAAA 301 ACCGAGACTA GTTCTCT Predicted gene structure (within gDNA segment 96687 to 94846): Exon 1 96077 95797 ( 281 n); cDNA 1 281 ( 281 n); score: 1.000 PPA cDNA 282 302 MATCH C08HBa0251H04.1- SGN-E309792- 1.000 281 0.886 C PGS_C08HBa0251H04.1-_SGN-E309792- (96077 95797) Alignment (genomic DNA sequence = upper lines): CTTGCTGCTG TTGAGCTGAC TGAATTTGGA TCTCCTGAAC CAGAATTGGA AATGGAAGAA 96018 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTGCTGCTG TTGAGCTGAC TGAATTTGGA TCTCCTGAAC CAGAATTGGA AATGGAAGAA 60 GAGGATGATC CTTATCCTCC ATCTGCAGTG GCCGTCGATG ATGGTTTCTG GTAACATCTC 95958 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGGATGATC CTTATCCTCC ATCTGCAGTG GCCGTCGATG ATGGTTTCTG GTAACATCTC 120 CCCAATGTAC AGTAGTCGCG CTTAATAATA TTCAAATAGG ATATAGAAAA ATGTAAACTA 95898 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCAATGTAC AGTAGTCGCG CTTAATAATA TTCAAATAGG ATATAGAAAA ATGTAAACTA 180 AAATGCAGGG CAAGAAGTGT ATCAGAAGGT GGTCTACATT GGGCTGCCAT CAACCTGACT 95838 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAATGCAGGG CAAGAAGTGT ATCAGAAGGT GGTCTACATT GGGCTGCCAT CAACCTGACT 240 TTGTATGTGA TATTTGTATT ATATATGTAT ATCTTCTGAC C 95797 |||||||||| |||||||||| |||||||||| |||||||||| | TTGTATGTGA TATTTGTATT ATATATGTAT ATCTTCTGAC C 281 hqPGS_C08HBa0251H04.1-_SGN-E309792- (96077 95797) ******************************************************************************** EST sequence 115 +strand 690 n (File: SGN-E332140+) 1 TGAAGACAAT CCAGATCTCG AGAAGCAGGC GTGGTTAGCT CTGTCTCAGG AAGAAGGAAA 61 ATCACCTCCT CCGGGTTTTA TTTTGAAGAG AATTGAAGGA ATGAAGAATG AGCAAGCGAT 121 ATCAGAAGTT CTATGCTGGT CAAGAGATCC TGTTCAAGTG AAGAGAATGG CTACTCGGAA 181 GGAAGATATT TATCAAGCCC TGCAGGGGGG AATATATAGC TTCCGACCTG GATCACAGGA 241 GTTCGTCAAC ACCCTGATGC ACTACAAAGT GCCAATGGCT TTAGTGTCTA CACGTCCAAG 301 AAAATATGTC GAGAATGCAA TTGGTACCAT TGGCTTTGAA GGGATATTTA GTGTAATTGT 361 AGCTGCAGAG GATGTTCACA GAGGGAAGCC TGATCCGGAG ATGTTTGTCT ATGCATCACA 421 GCTTCTACAA TTCATACCAG AGAGGTGTAT TGTGTTTGGG AACTCAAACC AAACCGTCGA 481 GGCTGCACAT GATGCTCAGA TGAAGTGTGT TGCAGTTGCT AGCAAACATC CAGTGTATGA 541 GCTTGGTGCT GCTGACTTGG TCGTGAGGCA CCTTGATGAG CTCTCGATCG TTGACCTAAA 601 GAACCTTGCT GCTGTTGAGC TGACTGAATT TGGATCTCCT GAACCAGAAT TGGAAATGGN 661 NAGAGAGGAT GATCCTTATC CCTCATCTGC Predicted gene structure (within gDNA segment 97281 to 95103): Exon 1 96681 95992 ( 690 n); cDNA 1 690 ( 690 n); score: 0.990 MATCH C08HBa0251H04.1- SGN-E332140+ 0.990 690 1.000 C PGS_C08HBa0251H04.1-_SGN-E332140+ (96681 95992) Alignment (genomic DNA sequence = upper lines): TGAAGACAAT CCAGATCTCG AGAAGCAGGC GTGGTTAGCT CTGTCTCAGG AAGAAGGAAA 96622 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAAGACAAT CCAGATCTCG AGAAGCAGGC GTGGTTAGCT CTGTCTCAGG AAGAAGGAAA 60 ATCACCTCCT CCGGGTTTTA TTTTGAAGAG AATTGAAGGA ATGAAGAATG AGCAAGCGAT 96562 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCACCTCCT CCGGGTTTTA TTTTGAAGAG AATTGAAGGA ATGAAGAATG AGCAAGCGAT 120 ATCAGAAGTT CTATGCTGGT CAAGAGATCC TGTTCAAGTG AAGAGAATGG CTACTCGGAA 96502 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCAGAAGTT CTATGCTGGT CAAGAGATCC TGTTCAAGTG AAGAGAATGG CTACTCGGAA 180 GGAAGATATT TATCAAGCCC TGCAGGGGGG AATATATAGC TTCCGACCTG GATCACAGGA 96442 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAAGATATT TATCAAGCCC TGCAGGGGGG AATATATAGC TTCCGACCTG GATCACAGGA 240 GTTCGTCAAC ACCCTGATGC ACTACAAAGT GCCAATGGCT TTAGTGTCTA CACGTCCAAG 96382 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTCGTCAAC ACCCTGATGC ACTACAAAGT GCCAATGGCT TTAGTGTCTA CACGTCCAAG 300 AAAATATGTC GAGAATGCAA TTGGTACCAT TGGCTTTGAA GGGATATTTA GTGTAATTGT 96322 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAATATGTC GAGAATGCAA TTGGTACCAT TGGCTTTGAA GGGATATTTA GTGTAATTGT 360 AGCTGCAGAG GATGTTCACA GAGGGAAGCC TGATCCGGAG ATGTTTGTCT ATGCATCACA 96262 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTGCAGAG GATGTTCACA GAGGGAAGCC TGATCCGGAG ATGTTTGTCT ATGCATCACA 420 GCTTCTACAA TTCATACCAG AGAGGTGTAT TGTGTTTGGG AACTCAAACC AAACCGTCGA 96202 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTCTACAA TTCATACCAG AGAGGTGTAT TGTGTTTGGG AACTCAAACC AAACCGTCGA 480 GGCTGCACAT GATGCTCAGA TGAAGTGTGT TGCAGTTGCT AGCAAACATC CAGTGTATGA 96142 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCTGCACAT GATGCTCAGA TGAAGTGTGT TGCAGTTGCT AGCAAACATC CAGTGTATGA 540 GCTTGGTGCT GCTGACTTGG TTGTGAGGCA CCTTGATGAG CTCTCGATCG TTGACCTAAA 96082 |||||||||| |||||||||| | |||||||| |||||||||| |||||||||| |||||||||| GCTTGGTGCT GCTGACTTGG TCGTGAGGCA CCTTGATGAG CTCTCGATCG TTGACCTAAA 600 GAACCTTGCT GCTGTTGAGC TGACTGAATT TGGATCTCCT GAACCAGAAT TGGAAATGGA 96022 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| GAACCTTGCT GCTGTTGAGC TGACTGAATT TGGATCTCCT GAACCAGAAT TGGAAATGGN 660 AGAAGAGGAT GATCCTTATC CTCCATCTGC 95992 ||||||| |||||||||| | ||||||| NAGAGAGGAT GATCCTTATC CCTCATCTGC 690 hqPGS_C08HBa0251H04.1-_SGN-E332140+ (96681 95992) ******************************************************************************** EST sequence 123 +strand 542 n (File: SGN-E206842+) 1 ATGGTTGAAT CAATTGGTGC AATATCACTT TTAGGGAGGG TGCCAGTTTA TGGAGTTAAT 61 TGTAGCAAAG ATGTGTCGAG TAAACGGAAG TCGATTGATG TATGTCGATT GCCTTCTGCT 121 GATTTTCTTG GCAGAAAGCT TGTTTGTGGT TCGAATTTAC TGAGATTAAG AGGTGATTGT 181 GTGAGGTTTT CGTTGATTAG AGGTCAGGCG ATGGAATTGA CCAAAGAGGC ATTTTCGTTC 241 AGAGAGGACG AGAAAATATC TAAAGATTTT CGTTATAGGA TTGAGACTGA TGTGGATAAA 301 AAGCCAGGGA AATGGCCACC AACGAATAAA GCTGATAATC CAGCTCTTCA TAATCCATTG 361 CTTAGACAAG AGAGGATGGG CTGTGGATGG TTAGCAGCTA TATTTGAATG GGAAGGTGTG 421 TTAACTGAAG ACAATCCAGA TCTCGAGAAG CAGGCGTGGT TAGCTCTGTC TCAGGAAGAA 481 GGAAAATCAC CTCCTCCGGG TTTTATTTTG AAGAGAATTG AAGGAATGAA GAATGAGCAA 541 GC Predicted gene structure (within gDNA segment 97706 to 95955): Exon 1 97106 96565 ( 542 n); cDNA 1 542 ( 542 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E206842+ 1.000 542 1.000 C PGS_C08HBa0251H04.1-_SGN-E206842+ (97106 96565) Alignment (genomic DNA sequence = upper lines): ATGGTTGAAT CAATTGGTGC AATATCACTT TTAGGGAGGG TGCCAGTTTA TGGAGTTAAT 97047 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGGTTGAAT CAATTGGTGC AATATCACTT TTAGGGAGGG TGCCAGTTTA TGGAGTTAAT 60 TGTAGCAAAG ATGTGTCGAG TAAACGGAAG TCGATTGATG TATGTCGATT GCCTTCTGCT 96987 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTAGCAAAG ATGTGTCGAG TAAACGGAAG TCGATTGATG TATGTCGATT GCCTTCTGCT 120 GATTTTCTTG GCAGAAAGCT TGTTTGTGGT TCGAATTTAC TGAGATTAAG AGGTGATTGT 96927 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATTTTCTTG GCAGAAAGCT TGTTTGTGGT TCGAATTTAC TGAGATTAAG AGGTGATTGT 180 GTGAGGTTTT CGTTGATTAG AGGTCAGGCG ATGGAATTGA CCAAAGAGGC ATTTTCGTTC 96867 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGAGGTTTT CGTTGATTAG AGGTCAGGCG ATGGAATTGA CCAAAGAGGC ATTTTCGTTC 240 AGAGAGGACG AGAAAATATC TAAAGATTTT CGTTATAGGA TTGAGACTGA TGTGGATAAA 96807 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAGAGGACG AGAAAATATC TAAAGATTTT CGTTATAGGA TTGAGACTGA TGTGGATAAA 300 AAGCCAGGGA AATGGCCACC AACGAATAAA GCTGATAATC CAGCTCTTCA TAATCCATTG 96747 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCCAGGGA AATGGCCACC AACGAATAAA GCTGATAATC CAGCTCTTCA TAATCCATTG 360 CTTAGACAAG AGAGGATGGG CTGTGGATGG TTAGCAGCTA TATTTGAATG GGAAGGTGTG 96687 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTTAGACAAG AGAGGATGGG CTGTGGATGG TTAGCAGCTA TATTTGAATG GGAAGGTGTG 420 TTAACTGAAG ACAATCCAGA TCTCGAGAAG CAGGCGTGGT TAGCTCTGTC TCAGGAAGAA 96627 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAACTGAAG ACAATCCAGA TCTCGAGAAG CAGGCGTGGT TAGCTCTGTC TCAGGAAGAA 480 GGAAAATCAC CTCCTCCGGG TTTTATTTTG AAGAGAATTG AAGGAATGAA GAATGAGCAA 96567 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGAAAATCAC CTCCTCCGGG TTTTATTTTG AAGAGAATTG AAGGAATGAA GAATGAGCAA 540 GC 96565 || GC 542 hqPGS_C08HBa0251H04.1-_SGN-E206842+ (97106 96565) ******************************************************************************** EST sequence 95 +strand 530 n (File: SGN-E264431+) 1 GATGGTTGAA TCAATTGGTG CAATATCACT TTTAGGGAGG GTGCCAGTTT ATGGAGTTAA 61 TTGTAGCAAA GATGTGTCGA GTAAACGGAA GTCGATTGAT GTATGTCGAT TGCCTTCTGC 121 TGATTTTCTT GGCAGAAAGC TTGTTTGTGG TTCGAATTTA CTGAGATTAA GAGGTGATTG 181 TGTGAGGTTT TCGTTGATTA GAGGTCAGGC GATGGAATTG ACCAAAGAGG CATTTTCGTT 241 CAGAGAGGAC GAGAAAATAT CTAAAGATTT TCGTTATAGG ATTGAGACTG ATGTGGATAA 301 AAAGCCAGGG AAATGGCCAC CAACGAATAA AGCTGATAAT CCAGCTCTTC ATAATCCATT 361 GCTTAGACAA GAGAGGATGG GCTGTGGATG GTTAGCAGCT ATATTTGAAT GGGAAGGTGT 421 GTTAACTGAA GACAATCCAG ATCTCGAGAA GCAGGCGTGG TTAGCTCTGT CTCAGGAAGA 481 AAGAAAATCA CCTCCTCCGG GTTTTATTTT GAAGAGAATT GAAGGAATGA Predicted gene structure (within gDNA segment 97707 to 95968): Exon 1 97107 96578 ( 530 n); cDNA 1 530 ( 530 n); score: 0.998 MATCH C08HBa0251H04.1- SGN-E264431+ 0.998 530 1.000 C PGS_C08HBa0251H04.1-_SGN-E264431+ (97107 96578) Alignment (genomic DNA sequence = upper lines): GATGGTTGAA TCAATTGGTG CAATATCACT TTTAGGGAGG GTGCCAGTTT ATGGAGTTAA 97048 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GATGGTTGAA TCAATTGGTG CAATATCACT TTTAGGGAGG GTGCCAGTTT ATGGAGTTAA 60 TTGTAGCAAA GATGTGTCGA GTAAACGGAA GTCGATTGAT GTATGTCGAT TGCCTTCTGC 96988 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTAGCAAA GATGTGTCGA GTAAACGGAA GTCGATTGAT GTATGTCGAT TGCCTTCTGC 120 TGATTTTCTT GGCAGAAAGC TTGTTTGTGG TTCGAATTTA CTGAGATTAA GAGGTGATTG 96928 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGATTTTCTT GGCAGAAAGC TTGTTTGTGG TTCGAATTTA CTGAGATTAA GAGGTGATTG 180 TGTGAGGTTT TCGTTGATTA GAGGTCAGGC GATGGAATTG ACCAAAGAGG CATTTTCGTT 96868 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGAGGTTT TCGTTGATTA GAGGTCAGGC GATGGAATTG ACCAAAGAGG CATTTTCGTT 240 CAGAGAGGAC GAGAAAATAT CTAAAGATTT TCGTTATAGG ATTGAGACTG ATGTGGATAA 96808 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGAGAGGAC GAGAAAATAT CTAAAGATTT TCGTTATAGG ATTGAGACTG ATGTGGATAA 300 AAAGCCAGGG AAATGGCCAC CAACGAATAA AGCTGATAAT CCAGCTCTTC ATAATCCATT 96748 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAGCCAGGG AAATGGCCAC CAACGAATAA AGCTGATAAT CCAGCTCTTC ATAATCCATT 360 GCTTAGACAA GAGAGGATGG GCTGTGGATG GTTAGCAGCT ATATTTGAAT GGGAAGGTGT 96688 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTAGACAA GAGAGGATGG GCTGTGGATG GTTAGCAGCT ATATTTGAAT GGGAAGGTGT 420 GTTAACTGAA GACAATCCAG ATCTCGAGAA GCAGGCGTGG TTAGCTCTGT CTCAGGAAGA 96628 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTAACTGAA GACAATCCAG ATCTCGAGAA GCAGGCGTGG TTAGCTCTGT CTCAGGAAGA 480 AGGAAAATCA CCTCCTCCGG GTTTTATTTT GAAGAGAATT GAAGGAATGA 96578 | |||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGAAAATCA CCTCCTCCGG GTTTTATTTT GAAGAGAATT GAAGGAATGA 530 hqPGS_C08HBa0251H04.1-_SGN-E264431+ (97107 96578) ******************************************************************************** EST sequence 108 +strand 502 n (File: SGN-E270165+) 1 TGCAATATCA CTTTTAGGGA GGGTGCCAGG TTATGGAGTT AGAGTGTAGC AAAGATGTGT 61 CGAGTAAACG GAAGTCGATT GATGTATGTC GATTGCCTTC TGCTGATTTT CTTGGCAGAA 121 AGCTTGTTTG TGGTTCGAAT TTACTGAGAT TAAGAGGTGA TTGTGTGAGG TTTTCGTTGA 181 TTAGAGGTCA GGCGATGGAA TTGACCAAAG AGGCATTTTC GTTCAGAGAG GACGAGAAAA 241 TATCTAAAGA TTTTCGTTAT AGGATTGAGA CTGATGTGGA TAAAAAGCCA GGGAAATGGC 301 CACCAACGAA TAAAGCTGAT AATCCAGCTC TTCATAATCC ATTGCTTAGA CAAGAGAGGA 361 TGGGCTGTGG ATGGTTAGCA GCTATATTTG AATGGGAAGG TGTGTTAACT GAAGACAATC 421 CAGATCTCGA GAAGCAGGCG TGGTTAGCTC TGTCTCANGA AGAAGGAAAA TCACCTCCTC 481 CGGGTTTTAT TTTGAAGAGA AT Predicted gene structure (within gDNA segment 97689 to 95979): Exon 1 97089 96589 ( 501 n); cDNA 1 502 ( 502 n); score: 0.989 MATCH C08HBa0251H04.1- SGN-E270165+ 0.989 501 0.998 C PGS_C08HBa0251H04.1-_SGN-E270165+ (97089 96589) Alignment (genomic DNA sequence = upper lines): TGCAATATCA CTTTTAGGGA GGGTGCCAGT TTATGGAGTT A-ATTGTAGC AAAGATGTGT 97031 |||||||||| |||||||||| ||||||||| |||||||||| | | |||||| |||||||||| TGCAATATCA CTTTTAGGGA GGGTGCCAGG TTATGGAGTT AGAGTGTAGC AAAGATGTGT 60 CGAGTAAACG GAAGTCGATT GATGTATGTC GATTGCCTTC TGCTGATTTT CTTGGCAGAA 96971 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGAGTAAACG GAAGTCGATT GATGTATGTC GATTGCCTTC TGCTGATTTT CTTGGCAGAA 120 AGCTTGTTTG TGGTTCGAAT TTACTGAGAT TAAGAGGTGA TTGTGTGAGG TTTTCGTTGA 96911 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTTGTTTG TGGTTCGAAT TTACTGAGAT TAAGAGGTGA TTGTGTGAGG TTTTCGTTGA 180 TTAGAGGTCA GGCGATGGAA TTGACCAAAG AGGCATTTTC GTTCAGAGAG GACGAGAAAA 96851 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAGAGGTCA GGCGATGGAA TTGACCAAAG AGGCATTTTC GTTCAGAGAG GACGAGAAAA 240 TATCTAAAGA TTTTCGTTAT AGGATTGAGA CTGATGTGGA TAAAAAGCCA GGGAAATGGC 96791 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATCTAAAGA TTTTCGTTAT AGGATTGAGA CTGATGTGGA TAAAAAGCCA GGGAAATGGC 300 CACCAACGAA TAAAGCTGAT AATCCAGCTC TTCATAATCC ATTGCTTAGA CAAGAGAGGA 96731 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACCAACGAA TAAAGCTGAT AATCCAGCTC TTCATAATCC ATTGCTTAGA CAAGAGAGGA 360 TGGGCTGTGG ATGGTTAGCA GCTATATTTG AATGGGAAGG TGTGTTAACT GAAGACAATC 96671 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGGCTGTGG ATGGTTAGCA GCTATATTTG AATGGGAAGG TGTGTTAACT GAAGACAATC 420 CAGATCTCGA GAAGCAGGCG TGGTTAGCTC TGTCTCAGGA AGAAGGAAAA TCACCTCCTC 96611 |||||||||| |||||||||| |||||||||| ||||||| || |||||||||| |||||||||| CAGATCTCGA GAAGCAGGCG TGGTTAGCTC TGTCTCANGA AGAAGGAAAA TCACCTCCTC 480 CGGGTTTTAT TTTGAAGAGA AT 96589 |||||||||| |||||||||| || CGGGTTTTAT TTTGAAGAGA AT 502 hqPGS_C08HBa0251H04.1-_SGN-E270165+ (97089 96589) ******************************************************************************** EST sequence 117 +strand 488 n (File: SGN-E206662+) 1 TGGTTGAATC AATTGGTGCA ATATCACTTT TAGGGAGGGT GCCAGTTTAT GGAGTTAATT 61 GTAGCAAAGA TGTGTCGAGT AAACGGAAGT CGATTGATGT ATGTCGATTG CCTTCTGCTG 121 ATTTTCTTGG CAGAAAGCTT GTTTGTGGTT CGAATTTACT GAGATTAAGA GGTGATTGTG 181 TGAGGTTTTC GTTGATTAGA GGTCAGGCGA TGGAATTGAC CAAAGAGGCA TTTTCGTTCA 241 GAGAGGACGA GAAAATATCT AAAGATTTTC GTTATAGGAT TGAGACTGAT GTGGATAAAA 301 AGCCAGGGAA ATGGCCACCA ACGAATAAAG CTGATAATCC AGCTCTTCAT AATCCATTGC 361 TTAGACAAGA GAGGATGGGC TGTGGATGGT TAGCAGCTAT ATTTGAATGG GAAGGTGTGT 421 TAACTGAAGA CAATCCAGAT CTCGAGAAGC AGGCGTGGTT AGCTCTGTCT CAGGAAGAAG 481 GAAAATCA Predicted gene structure (within gDNA segment 97705 to 96008): Exon 1 97105 96618 ( 488 n); cDNA 1 488 ( 488 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E206662+ 1.000 488 1.000 C PGS_C08HBa0251H04.1-_SGN-E206662+ (97105 96618) Alignment (genomic DNA sequence = upper lines): TGGTTGAATC AATTGGTGCA ATATCACTTT TAGGGAGGGT GCCAGTTTAT GGAGTTAATT 97046 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTGAATC AATTGGTGCA ATATCACTTT TAGGGAGGGT GCCAGTTTAT GGAGTTAATT 60 GTAGCAAAGA TGTGTCGAGT AAACGGAAGT CGATTGATGT ATGTCGATTG CCTTCTGCTG 96986 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTAGCAAAGA TGTGTCGAGT AAACGGAAGT CGATTGATGT ATGTCGATTG CCTTCTGCTG 120 ATTTTCTTGG CAGAAAGCTT GTTTGTGGTT CGAATTTACT GAGATTAAGA GGTGATTGTG 96926 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTTCTTGG CAGAAAGCTT GTTTGTGGTT CGAATTTACT GAGATTAAGA GGTGATTGTG 180 TGAGGTTTTC GTTGATTAGA GGTCAGGCGA TGGAATTGAC CAAAGAGGCA TTTTCGTTCA 96866 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAGGTTTTC GTTGATTAGA GGTCAGGCGA TGGAATTGAC CAAAGAGGCA TTTTCGTTCA 240 GAGAGGACGA GAAAATATCT AAAGATTTTC GTTATAGGAT TGAGACTGAT GTGGATAAAA 96806 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGAGGACGA GAAAATATCT AAAGATTTTC GTTATAGGAT TGAGACTGAT GTGGATAAAA 300 AGCCAGGGAA ATGGCCACCA ACGAATAAAG CTGATAATCC AGCTCTTCAT AATCCATTGC 96746 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCCAGGGAA ATGGCCACCA ACGAATAAAG CTGATAATCC AGCTCTTCAT AATCCATTGC 360 TTAGACAAGA GAGGATGGGC TGTGGATGGT TAGCAGCTAT ATTTGAATGG GAAGGTGTGT 96686 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAGACAAGA GAGGATGGGC TGTGGATGGT TAGCAGCTAT ATTTGAATGG GAAGGTGTGT 420 TAACTGAAGA CAATCCAGAT CTCGAGAAGC AGGCGTGGTT AGCTCTGTCT CAGGAAGAAG 96626 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAACTGAAGA CAATCCAGAT CTCGAGAAGC AGGCGTGGTT AGCTCTGTCT CAGGAAGAAG 480 GAAAATCA 96618 |||||||| GAAAATCA 488 hqPGS_C08HBa0251H04.1-_SGN-E206662+ (97105 96618) ******************************************************************************** EST sequence 8 +strand 469 n (File: SGN-E315454+) 1 GGCGGCTCGC TTTACCGACT CCGGCGACGG CGGAAAACCT TATCCGACGG CAAAAATGGG 61 TAAGAAACCG TCGAGGAAGA ATGGAGTTGG TAAAGCATCT GGTTCAATTA CATTGCGAGA 121 AGAATCAGGT GTGAAGAAGA AACAAACTCT TGTTAATGCG AAATCGATGC TGAAATTGGA 181 GCATATAAAG GATATTGCTA ACTGGACTAG TGGAGAAGGT TCCATACCTT CACTTGCTGC 241 GTTTTTTGGG CAGAGATTAG CTGTATCAGC TGAGTCTTTG GGTGTTTCAC CTGACCCTTC 301 ATTATTAACC TGTCAAAGGT GTGAATCTAT CCTTCAGGCT GGCTACAATA GTACAGCACG 361 GATTGAGAAG AACAAAAGAA AGGCACGAAA GAAACGNCAA ACATCAGGTA TTCCCTCGAA 421 GAACTCAGTT GTATATGAGT GTCATTTCTG CTCACATCGA AATCTAAAG Predicted gene structure (within gDNA segment 99844 to 102320): Exon 1 100444 100761 ( 318 n); cDNA 1 318 ( 318 n); score: 1.000 Intron 1 100762 101559 ( 798 n); Pd: 0.899 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 2 101560 101710 ( 151 n); cDNA 319 469 ( 151 n); score: 0.987 MATCH C08HBa0251H04.1+ SGN-E315454+ 0.996 469 1.000 C PGS_C08HBa0251H04.1+_SGN-E315454+ (100444 100761,101560 101710) Alignment (genomic DNA sequence = upper lines): GGCGGCTCGC TTTACCGACT CCGGCGACGG CGGAAAACCT TATCCGACGG CAAAAATGGG 100503 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGCGGCTCGC TTTACCGACT CCGGCGACGG CGGAAAACCT TATCCGACGG CAAAAATGGG 60 TAAGAAACCG TCGAGGAAGA ATGGAGTTGG TAAAGCATCT GGTTCAATTA CATTGCGAGA 100563 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAGAAACCG TCGAGGAAGA ATGGAGTTGG TAAAGCATCT GGTTCAATTA CATTGCGAGA 120 AGAATCAGGT GTGAAGAAGA AACAAACTCT TGTTAATGCG AAATCGATGC TGAAATTGGA 100623 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAATCAGGT GTGAAGAAGA AACAAACTCT TGTTAATGCG AAATCGATGC TGAAATTGGA 180 GCATATAAAG GATATTGCTA ACTGGACTAG TGGAGAAGGT TCCATACCTT CACTTGCTGC 100683 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCATATAAAG GATATTGCTA ACTGGACTAG TGGAGAAGGT TCCATACCTT CACTTGCTGC 240 GTTTTTTGGG CAGAGATTAG CTGTATCAGC TGAGTCTTTG GGTGTTTCAC CTGACCCTTC 100743 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTTTTTTGGG CAGAGATTAG CTGTATCAGC TGAGTCTTTG GGTGTTTCAC CTGACCCTTC 300 ATTATTAACC TGTCAAAGGT TTGAGCTTTT TCATATAATT TTGGTTTGAT TTGTCTTGCA 100803 |||||||||| |||||||| ATTATTAACC TGTCAAAG.. .......... .......... .......... .......... 318 TTTATGAATG CATACTTTGT TAATCTTGTG ATTGGTTAAA AAATGGATAT TTGTTGACGT 100863 .......... .......... .......... .......... .......... .......... 318 TGGGTATAGT AAGAGAGATA GGACTAGAGA TGAGTATATT CGGAATAAGG TAGGAGTGGT 100923 .......... .......... .......... .......... .......... .......... 318 TTTGGTTGAG GACAAGATGC GGGAAGCTAG ACTGAGATGG TTCAGGCATG GAGATGTAGA 100983 .......... .......... .......... .......... .......... .......... 318 TGCACAAATG TGTGGAGGTG TGAGAGAGTG GCTATGGATG GTTTAGAGAG AGGTGATTAG 101043 .......... .......... .......... .......... .......... .......... 318 ACTAGCCATA ACGTAGTTTT TAGCTTACTG AGGACATGAC CTTAGATTGG AGCATATCGA 101103 .......... .......... .......... .......... .......... .......... 318 GGATACAAAT TAGCGCAGGA GGTTATAGAA GATCACTAGT TAGTAATGTG TTGTATTGCT 101163 .......... .......... .......... .......... .......... .......... 318 ATCCATCCAT ACTAGTAGTT GTAATTTCTG TTACTATCTT TTGTTTCTTG TACATTAATT 101223 .......... .......... .......... .......... .......... .......... 318 ATCGTATTGT TTCGTTGTAC TGTTCAGAAT GCGCTGTTAT GCTTACACTT TAGCTTATTC 101283 .......... .......... .......... .......... .......... .......... 318 TCTTTCTGGA CTGCTTTGGA CTGTTTTCTT TGGAGTCTGT CGAAAACAAC CTAAGGTCTG 101343 .......... .......... .......... .......... .......... .......... 318 CCTGCACTAT ATCCTCCTAG ACATCTCTTT TTGACATTTG TACGAACCAA TGTAGAAATG 101403 .......... .......... .......... .......... .......... .......... 318 ACATCCAATG ATGAACTGCA AACGGATCAC TGTACGCATC TAAAGATCTC TTGAGTCTAT 101463 .......... .......... .......... .......... .......... .......... 318 CGGAAACAAC TCTCTATCCT AATGTTGAAT GAGTCATTGT CGACTTCTCA TTCTTTTTCT 101523 .......... .......... .......... .......... .......... .......... 318 ACGCTTGCAT ACTAACTTGT AATTGATATT TCACAGGTGT GAATCTATCC TTCAGGCTGG 101583 |||| |||||||||| |||||||||| .......... .......... .......... ......GTGT GAATCTATCC TTCAGGCTGG 342 CTACAATAGT ACAGCACGGA TTGAGAAGAA CAAAAGAAAG GCACGAAAGA AACGCAAAAC 101643 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| |||| CTACAATAGT ACAGCACGGA TTGAGAAGAA CAAAAGAAAG GCACGAAAGA AACGNCAAAC 402 ATCAGGTATT CCCTCGAAGA ACTCAGTTGT ATATGAGTGT CATTTCTGCT CACATCGAAA 101703 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCAGGTATT CCCTCGAAGA ACTCAGTTGT ATATGAGTGT CATTTCTGCT CACATCGAAA 462 TCTAAAG 101710 ||||||| TCTAAAG 469 hqPGS_C08HBa0251H04.1+_SGN-E315454+ (100444 100761,101560 101710) ******************************************************************************** EST sequence 100 +strand 636 n (File: SGN-E268933+) 1 CAGTTGACAC AATTAAGACT GTTATTCAGT CATGTCCCAA TGATCAGAAG CCACTTTATT 61 ATATCGGCAG ATCATTCATT TCTGAAAGAG GAGTAACAGC ACTTTATCGG GGAATTTCGA 121 CCAATCTTGC TTCGTCTGCA CCCATATCTG CACTTTACAC CTTTACATAC GAATCAGTTA 181 AAGGGGCCTT ACTACCTCTT TTTCCTAAGG AATGCCACTC TTTTGCACAC TGCTTGGCTG 241 GGGGTTCTGC AAGCATTGCC ACTTCATTCA TTTTCACTCC GAGTGAGCGC ATTAAACAAC 301 AGATGCAAGT GGGCTCCCAC TATAAGAACT GCTGGAGTGC CTTAATTGTG ATTACAAGAA 361 GTGGTGGTTT GCCTTCTCTG TATGCTGGAT GGAAAGCTGT ACTCTGGAGG AATATTCCAC 421 ACTCAATCAT TAAGTTCTAT ACATATGAAA GATTGAAGGA GTTGAGGTTA TCATCAGTTC 481 AACTACGCAA CCAAAATGAC ACACTAATGA CACTTGCCTG TGGAGGATTA GCTGGCTCTA 541 CTGCTGCACT ATTCACAACT CCCTTTGATG TTGTCAAGAC AAGATTACAG ACACAGATTC 601 CTGGATCTAT GACTCAGTTT GGTGTATTCG GCACAC Predicted gene structure (within gDNA segment 127067 to 122261): Exon 1 126467 126378 ( 90 n); cDNA 1 90 ( 90 n); score: 1.000 Intron 1 126377 126257 ( 121 n); Pd: 0.937 (s: 1.00), Pa: 0.946 (s: 1.00) Exon 2 126256 126138 ( 119 n); cDNA 91 209 ( 119 n); score: 1.000 Intron 2 126137 125880 ( 258 n); Pd: 0.988 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 125879 125755 ( 125 n); cDNA 210 334 ( 125 n); score: 1.000 Intron 3 125754 123851 (1904 n); Pd: 0.997 (s: 1.00), Pa: 0.763 (s: 1.00) Exon 4 123850 123751 ( 100 n); cDNA 335 434 ( 100 n); score: 1.000 Intron 4 123750 123590 ( 161 n); Pd: 0.998 (s: 1.00), Pa: 0.932 (s: 1.00) Exon 5 123589 123512 ( 78 n); cDNA 435 512 ( 78 n); score: 1.000 Intron 5 123511 123267 ( 245 n); Pd: 0.976 (s: 1.00), Pa: 0.822 (s: 1.00) Exon 6 123266 123183 ( 84 n); cDNA 513 596 ( 84 n); score: 1.000 Intron 6 123182 122911 ( 272 n); Pd: 0.998 (s: 1.00), Pa: 0.995 (s: 1.00) Exon 7 122910 122871 ( 40 n); cDNA 597 636 ( 40 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E268933+ 1.000 636 1.000 C PGS_C08HBa0251H04.1-_SGN-E268933+ (126467 126378,126256 126138,125879 125755,123850 123751,123589 123512,123266 123183,122910 122871) Alignment (genomic DNA sequence = upper lines): CAGTTGACAC AATTAAGACT GTTATTCAGT CATGTCCCAA TGATCAGAAG CCACTTTATT 126408 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGTTGACAC AATTAAGACT GTTATTCAGT CATGTCCCAA TGATCAGAAG CCACTTTATT 60 ATATCGGCAG ATCATTCATT TCTGAAAGAG GTCTATTTAT TTGAAGCTGC ACATCACTTT 126348 |||||||||| |||||||||| |||||||||| ATATCGGCAG ATCATTCATT TCTGAAAGAG .......... .......... .......... 90 TCAATATGAA AATTCCTTAC ATGCAATGAG ATGCTTCTCT TTCTTAGAGC CTATGTTTAA 126288 .......... .......... .......... .......... .......... .......... 90 ACCTTTGAAA TTGTTGGTCA ACTTATCTCA GGAGTAACAG CACTTTATCG GGGAATTTCG 126228 ||||||||| |||||||||| |||||||||| .......... .......... .......... .GAGTAACAG CACTTTATCG GGGAATTTCG 119 ACCAATCTTG CTTCGTCTGC ACCCATATCT GCACTTTACA CCTTTACATA CGAATCAGTT 126168 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAATCTTG CTTCGTCTGC ACCCATATCT GCACTTTACA CCTTTACATA CGAATCAGTT 179 AAAGGGGCCT TACTACCTCT TTTTCCTAAG GTGAATGTTT TGTGTTCTTC TTTTTCATCC 126108 |||||||||| |||||||||| |||||||||| AAAGGGGCCT TACTACCTCT TTTTCCTAAG .......... .......... .......... 209 TTTTATGTTA AACTTCTCCT CAGAATTTCT GTTTGCACTA CCCAAGCAAG CCGAGAATGA 126048 .......... .......... .......... .......... .......... .......... 209 ATTGGAAAAA AAATTGAAAA GAGACTGTTA GGCTTGCGAA GTTGTTATAA TTTCTTTAAA 125988 .......... .......... .......... .......... .......... .......... 209 GTAGTAAAGG AGAAGCTGGT CTATCTCCGT TGGTAACCAT AGATAGTATG ATTCTTTTTA 125928 .......... .......... .......... .......... .......... .......... 209 CAAAGGAAAT TAGTGTATTC CTGATATTAC ACATTTCTAT TACCACAGGA ATGCCACTCT 125868 || |||||||||| .......... .......... .......... .......... ........GA ATGCCACTCT 221 TTTGCACACT GCTTGGCTGG GGGTTCTGCA AGCATTGCCA CTTCATTCAT TTTCACTCCG 125808 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGCACACT GCTTGGCTGG GGGTTCTGCA AGCATTGCCA CTTCATTCAT TTTCACTCCG 281 AGTGAGCGCA TTAAACAACA GATGCAAGTG GGCTCCCACT ATAAGAACTG CTGGTAAGTT 125748 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| AGTGAGCGCA TTAAACAACA GATGCAAGTG GGCTCCCACT ATAAGAACTG CTG....... 334 ATGTTCCTGA AATACATTAG TCATCCCATA TCTGATTCCA CCAGCTTTTG GATCTGTCTG 125688 .......... .......... .......... .......... .......... .......... 334 ATTCTGTTTC CCACAGTTTA ATGATTTGAG AATGCTCCAA GCCTTTGGGT GCTTCCTGTC 125628 .......... .......... .......... .......... .......... .......... 334 TGTGTTTAAG TATGCATCTG TTGCCTAAGT ATCCTGCAAC TCATGTTCTT TTTCAATATA 125568 .......... .......... .......... .......... .......... .......... 334 TATATATATA TATATATATA CACTCATATG TTCCTCAAGG TCTATCCTGG ATAGAATTGA 125508 .......... .......... .......... .......... .......... .......... 334 AGAAAATTCT CAATATGATC TTTGTTGCTA CAACATGTTT AAGTTTCAGT TGGTCCTCAG 125448 .......... .......... .......... .......... .......... .......... 334 TATTATGAAG TCCGTCTCAC AAAATTGAGA TAACTCACAG GAAAAGGTAA ACTGAGGAAT 125388 .......... .......... .......... .......... .......... .......... 334 ACTTGATTAA CCTCAATAGG GTTATTGATG TCTTAAGTTA ATGCTTCTTT GTTATGTGAC 125328 .......... .......... .......... .......... .......... .......... 334 GAGCACAAAT TAGACATTAT TTTCAAGCGC TTCGTTAGTC AAATATAGTA TAGAATAATT 125268 .......... .......... .......... .......... .......... .......... 334 ATTGTCTCCA CATGGATTGG TACATTAATG CCTCAAACGA ACTTTGCTAA GTTTTTATAT 125208 .......... .......... .......... .......... .......... .......... 334 GGAAAATATT AACTGTAATT TGATGCGGTA TTACACCATG ACTATCAAAT ATACTTTTAA 125148 .......... .......... .......... .......... .......... .......... 334 CTAGTGTTGA TTTAAATGAA CTTGGGAATT ATGAAATAAC GATTGATAGG AGAATTAAAT 125088 .......... .......... .......... .......... .......... .......... 334 GATAATGACT AGACAGGTGC AAAAGAAATA AGCAGGATCA ACTAGTTTTC AAGGTTCTTA 125028 .......... .......... .......... .......... .......... .......... 334 TCTGAGTTTT CTTTGCTTTT GGCAAATTCA ATGACTGACT AGCATGTCCT CTATATTAGG 124968 .......... .......... .......... .......... .......... .......... 334 ACACGTCTTT CGAATGAATT TGATATCTTC AAACTCAACT AAGTAAATGC AGAAGAACAT 124908 .......... .......... .......... .......... .......... .......... 334 TTGCAAGAAT AAGCCAATGG AATTGTAAAA GAAAAACTTC CCATGAATAT TTTCCTATAT 124848 .......... .......... .......... .......... .......... .......... 334 TAATTTTAAG AACAATTCAA GAAGCTCTTT CAATTACTGA AAAAATTGCT ATACTAAATC 124788 .......... .......... .......... .......... .......... .......... 334 ATACTCTAGT AGCATAGATT GTCAGTATTA AGCTTCTCTT TTGATTAAAC TAAATAAGAA 124728 .......... .......... .......... .......... .......... .......... 334 TAACTATGCT TATTCTTCAT ACTCCATTAA CAAAATCTAA CAATTTAAGC AAGTCGAATC 124668 .......... .......... .......... .......... .......... .......... 334 CATGAATTAC ACCTAACACA CCATGTCACA AACCCTATGA GGAACTACTC CATAAAATTA 124608 .......... .......... .......... .......... .......... .......... 334 GACAACCTAG TCAAAGAAGA AATAAAATTA GAGTTCATTA CAACGAACTA TCGATCCAAA 124548 .......... .......... .......... .......... .......... .......... 334 CTGCTGTATT GAATTGAAAT GATGATGAAA GTATTGAAAT TCGTTGTCTT CAACCCTTAA 124488 .......... .......... .......... .......... .......... .......... 334 TGCTTACTCT GACTTCCCAA CATCCAGACC CCTATTACTT GCGTTTTTGG AGTATTTATA 124428 .......... .......... .......... .......... .......... .......... 334 CCATGTACAA AAGATCCCCA ACCAAAATAC CCTTCAAAAG ACAACTTGAA AAAAATCTGG 124368 .......... .......... .......... .......... .......... .......... 334 CAACAAAATG CCTGATGCAA CATGTTGGCC CAGGCCAATT TTCCAGGCCT CTGGATTTCT 124308 .......... .......... .......... .......... .......... .......... 334 CGGCAGGGAG CAGCACACAA GGCAGGCACG GTGCACCAAG CCATTTTCCA GTGTCGTCAT 124248 .......... .......... .......... .......... .......... .......... 334 CTACTTCATT CTTTCGCATG CATCTCGGTC CTTGAACCTT GAACTTGATC CCGTTTGAAA 124188 .......... .......... .......... .......... .......... .......... 334 TCCTCTTGGA CCTCTACTTA GACTTCAAAG TACCAAGACA TCTATCTAAG CTCCAAAATT 124128 .......... .......... .......... .......... .......... .......... 334 GCTCAAAAAT CTTAGAAACA AGTCCTACAA CCAAAACACA TGTAATAAGC ACAAAGCATT 124068 .......... .......... .......... .......... .......... .......... 334 AAACATTATA AATCAAACAC TATTAAAATG TAGTTAAATA AGACGAAATG TATCTAGGAA 124008 .......... .......... .......... .......... .......... .......... 334 CTTATGTTTC TTGCATACCA TCATTATGTG GTCTGCTTTA ACCCAGTAAT TCTGTTATAG 123948 .......... .......... .......... .......... .......... .......... 334 TCATTTGCTA CTTCAAATCA GTACTCTAAG GAGAGGAGGA CTATTATTAC CTACTGACAA 123888 .......... .......... .......... .......... .......... .......... 334 GTGACAACAT CTTGCTTTCT CATTTTTGCA TCCTCAGGAG TGCCTTAATT GTGATTACAA 123828 ||| |||||||||| |||||||||| .......... .......... .......... .......GAG TGCCTTAATT GTGATTACAA 357 GAAGTGGTGG TTTGCCTTCT CTGTATGCTG GATGGAAAGC TGTACTCTGG AGGAATATTC 123768 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGGTGG TTTGCCTTCT CTGTATGCTG GATGGAAAGC TGTACTCTGG AGGAATATTC 417 CACACTCAAT CATTAAGGTA AGTTTTGACT TTAGCAATCT GTCCCTGTGC CCTTTTGGAG 123708 |||||||||| ||||||| CACACTCAAT CATTAAG... .......... .......... .......... .......... 434 AGAATCGAGG AGCAGGAAGT TGATTGCTTT ATATTCTCAT TCATCCAAGT TTGACATCAT 123648 .......... .......... .......... .......... .......... .......... 434 CATTCTTTTT AGCCTTCTAT TGCTCTTTTA TATTTTACAT TACCATTTTG ACTGGCAGTT 123588 || .......... .......... .......... .......... .......... ........TT 436 CTATACATAT GAAAGATTGA AGGAGTTGAG GTTATCATCA GTTCAACTAC GCAACCAAAA 123528 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTATACATAT GAAAGATTGA AGGAGTTGAG GTTATCATCA GTTCAACTAC GCAACCAAAA 496 TGACACACTA ATGACAGTTA GTTTTCTTAC TAATGAGTAG TCCTATGTCT ACTTTTCATC 123468 |||||||||| |||||| TGACACACTA ATGACA.... .......... .......... .......... .......... 512 TTTGCCATTA TCGGGCTCAT GATAGATAAT TTGCTGGCAC TTACATTCTC ACATTTACTA 123408 .......... .......... .......... .......... .......... .......... 512 TTGGCTTTAA GGAATGATGG TCAAGGTTAT CGTTTTAGTT AGTTAAGTTT GTAGTTGAGT 123348 .......... .......... .......... .......... .......... .......... 512 CAATGGAGCC TTAGTGTTCT TGGTTTCATT GTCTATTGCA AAACAATAAG GTTTTCCTCA 123288 .......... .......... .......... .......... .......... .......... 512 GTTGTTTTCT CCTCCTTCCA GCTTGCCTGT GGAGGATTAG CTGGCTCTAC TGCTGCACTA 123228 ||||||||| |||||||||| |||||||||| |||||||||| .......... .......... .CTTGCCTGT GGAGGATTAG CTGGCTCTAC TGCTGCACTA 551 TTCACAACTC CCTTTGATGT TGTCAAGACA AGATTACAGA CACAGGTAAT TCATCAAATG 123168 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TTCACAACTC CCTTTGATGT TGTCAAGACA AGATTACAGA CACAG..... .......... 596 TGTTTCCTTC TTGTTCACTA GATGTCAGAA TGTCTATAGT TTCCTTGAAA CTGGGATTAA 123108 .......... .......... .......... .......... .......... .......... 596 GTTAAATCCT TCTGAAAGTG TTCTGTTGCT GTGCTTCACA TCCCTTTTTC CTATTTAATT 123048 .......... .......... .......... .......... .......... .......... 596 GATTGTTATT TATGAAATTG ATTCAAGACT GTTATGAGTG CCTTTGGAAA GCGTCAGCTA 122988 .......... .......... .......... .......... .......... .......... 596 CTGTACTTAA CATCTTACCC CTTACACTAT TTGTGTGTCT GATTTGTAAA GTTCCTAAAT 122928 .......... .......... .......... .......... .......... .......... 596 AAGTCTTGGT TGTGCAGATT CCTGGATCTA TGACTCAGTT TGGTGTATTC GGCACAC 122871 ||| |||||||||| |||||||||| |||||||||| ||||||| .......... .......ATT CCTGGATCTA TGACTCAGTT TGGTGTATTC GGCACAC 636 hqPGS_C08HBa0251H04.1-_SGN-E268933+ (126467 126378,126256 126138,125879 125755,123850 123751,123589 123512,123266 123183,122910 122871) ******************************************************************************** EST sequence 113 +strand 651 n (File: SGN-E342027+) 1 TGGTTCATTG TTAAGTCAGA TGGCAAAGCA TGATGAGATC GACAGGGTGT CAACAGGCAC 61 TTCATCTATA AGGCACTATA ATGAAGTTTT TCATGACAAA AAGTCAAACA CACTAGGGAT 121 TTCACATTCC GAAAATAACT TAGTCTTCCA TGAGTACTCG GTCACTCCAA GTTGTTCTGC 181 TAGTGTACAT GACAAAATTA ATGCTAAGGA TCACATATAT GATTCTGCCA TTAATGAAAA 241 AGTAGAACTG GAGGAAGAGC TCAGTTCTGA AGTACAGAGA TCTGTGGCAA AGGAAAAGCC 301 TCTGTATGCA CTTGCAAAAC AAGAGCATGC TTTTGCAGGA GCAATGGCTG GAATTTTTGT 361 CAGCCTTTGT CTTCATCCAG TTGACACAAT TAAGACTGTT ATTCAGTCAT GTCCCAATGA 421 TCAGAAGCCA CTTTATTATA TCGGCAGATC ATTCATTTCT GAAAGAGGAG TAACAGCACT 481 TTATCGGGGA ATTTCGACCA ATCTTGCTTC GTCTGCACCC ATATCTGCAC TTTACACCTT 541 TACATACGAA TCAGTTAAAG GGGCCTTACT ACCTCTTTTT CCTAAGGAAT GCCACTCTTT 601 TGCACACTGC TTGGCTGGGG GGTTCTGCAA GCATTGCCAC TTCATTCATT T Predicted gene structure (within gDNA segment 127444 to 125206): Exon 1 126844 126378 ( 467 n); cDNA 1 467 ( 467 n); score: 1.000 Intron 1 126377 126257 ( 121 n); Pd: 0.937 (s: 1.00), Pa: 0.946 (s: 1.00) Exon 2 126256 126138 ( 119 n); cDNA 468 586 ( 119 n); score: 1.000 Intron 2 126137 125880 ( 258 n); Pd: 0.988 (s: 1.00), Pa: 0.991 (s: 0.95) Exon 3 125879 125816 ( 64 n); cDNA 587 651 ( 65 n); score: 0.961 MATCH C08HBa0251H04.1- SGN-E342027+ 0.996 650 0.998 C PGS_C08HBa0251H04.1-_SGN-E342027+ (126844 126378,126256 126138,125879 125816) Alignment (genomic DNA sequence = upper lines): TGGTTCATTG TTAAGTCAGA TGGCAAAGCA TGATGAGATC GACAGGGTGT CAACAGGCAC 126785 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTCATTG TTAAGTCAGA TGGCAAAGCA TGATGAGATC GACAGGGTGT CAACAGGCAC 60 TTCATCTATA AGGCACTATA ATGAAGTTTT TCATGACAAA AAGTCAAACA CACTAGGGAT 126725 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCATCTATA AGGCACTATA ATGAAGTTTT TCATGACAAA AAGTCAAACA CACTAGGGAT 120 TTCACATTCC GAAAATAACT TAGTCTTCCA TGAGTACTCG GTCACTCCAA GTTGTTCTGC 126665 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCACATTCC GAAAATAACT TAGTCTTCCA TGAGTACTCG GTCACTCCAA GTTGTTCTGC 180 TAGTGTACAT GACAAAATTA ATGCTAAGGA TCACATATAT GATTCTGCCA TTAATGAAAA 126605 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGTGTACAT GACAAAATTA ATGCTAAGGA TCACATATAT GATTCTGCCA TTAATGAAAA 240 AGTAGAACTG GAGGAAGAGC TCAGTTCTGA AGTACAGAGA TCTGTGGCAA AGGAAAAGCC 126545 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGTAGAACTG GAGGAAGAGC TCAGTTCTGA AGTACAGAGA TCTGTGGCAA AGGAAAAGCC 300 TCTGTATGCA CTTGCAAAAC AAGAGCATGC TTTTGCAGGA GCAATGGCTG GAATTTTTGT 126485 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGTATGCA CTTGCAAAAC AAGAGCATGC TTTTGCAGGA GCAATGGCTG GAATTTTTGT 360 CAGCCTTTGT CTTCATCCAG TTGACACAAT TAAGACTGTT ATTCAGTCAT GTCCCAATGA 126425 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGCCTTTGT CTTCATCCAG TTGACACAAT TAAGACTGTT ATTCAGTCAT GTCCCAATGA 420 TCAGAAGCCA CTTTATTATA TCGGCAGATC ATTCATTTCT GAAAGAGGTC TATTTATTTG 126365 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TCAGAAGCCA CTTTATTATA TCGGCAGATC ATTCATTTCT GAAAGAG... .......... 467 AAGCTGCACA TCACTTTTCA ATATGAAAAT TCCTTACATG CAATGAGATG CTTCTCTTTC 126305 .......... .......... .......... .......... .......... .......... 467 TTAGAGCCTA TGTTTAAACC TTTGAAATTG TTGGTCAACT TATCTCAGGA GTAACAGCAC 126245 || |||||||||| .......... .......... .......... .......... ........GA GTAACAGCAC 479 TTTATCGGGG AATTTCGACC AATCTTGCTT CGTCTGCACC CATATCTGCA CTTTACACCT 126185 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTATCGGGG AATTTCGACC AATCTTGCTT CGTCTGCACC CATATCTGCA CTTTACACCT 539 TTACATACGA ATCAGTTAAA GGGGCCTTAC TACCTCTTTT TCCTAAGGTG AATGTTTTGT 126125 |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TTACATACGA ATCAGTTAAA GGGGCCTTAC TACCTCTTTT TCCTAAG... .......... 586 GTTCTTCTTT TTCATCCTTT TATGTTAAAC TTCTCCTCAG AATTTCTGTT TGCACTACCC 126065 .......... .......... .......... .......... .......... .......... 586 AAGCAAGCCG AGAATGAATT GGAAAAAAAA TTGAAAAGAG ACTGTTAGGC TTGCGAAGTT 126005 .......... .......... .......... .......... .......... .......... 586 GTTATAATTT CTTTAAAGTA GTAAAGGAGA AGCTGGTCTA TCTCCGTTGG TAACCATAGA 125945 .......... .......... .......... .......... .......... .......... 586 TAGTATGATT CTTTTTACAA AGGAAATTAG TGTATTCCTG ATATTACACA TTTCTATTAC 125885 .......... .......... .......... .......... .......... .......... 586 CACAGGAATG CCACTCTTTT GCACACTGCT TGGCT-GGGG GTTCTGCAAG CATTGCCACT 125826 ||||| |||||||||| |||||||||| ||||| |||| |||||||||| |||||||||| .....GAATG CCACTCTTTT GCACACTGCT TGGCTGGGGG GTTCTGCAAG CATTGCCACT 641 TCATTCATTT 125816 |||||||||| TCATTCATTT 651 hqPGS_C08HBa0251H04.1-_SGN-E342027+ (126844 126378,126256 126138,125879 125816) ******************************************************************************** EST sequence 96 +strand 767 n (File: SGN-E318360+) 1 CACAACTCAT TGTTTTACCA ATTATAGGAC TATGGAAGCT GAAACATCCC AATTCTGAAT 61 TTGGTATTTT TTTTCTTCAT TAACCCTACT AACTTGGGGC TGTCATGGGG GAAATTTGCA 121 TCCAATTTCT CTCTGATTTG CCCCAGGTGA AAGATGATTC ACAGCATTCT TGGCACATTG 181 TTTTCAGCTG CTACAAAACA GTCAATTTCA TTCAGGTGTA ATTTGTTACT ACTTGATTTG 241 TTGTGTCCAA TGGAACTAGT GGCTTGGGAC TAGTAAGAGG AAGGAAGCTT TGACAGTTGG 301 CAATCTTCTA GCATTTTATA TTTTGGAGAA CATTTTAAAT CATTGTGATA ATATGGCTGG 361 GGGAAGTCAA CCACGCCAAA GTGACAAACC TTCAATCAGA TACAGGTGTA ATCTACTTGA 421 TGGGGCACTG TTTGAAATCA CTGATTTAGA CCGACAGAGT AATGCCCCTA TTTCTTCTGG 481 AAATAAGGAG AACCCTAAAA ATTCTGACTC TGAATCTTCT AAGATAATGA GCACAAGTGA 541 GCTTATATCA GCAGTTGGAA GTATATGGTG TTCTGCAGCT GGCCCTGTTA CACGAATCCT 601 ATCTAAAGGA AGTTCTAGAT GCAATAATAC CGATCACCAA GAAAACAACA TATTTGGTTA 661 CTCCACTGCA GATAAAATTT CNTGGTGTTT GTTTTTCACC TCCTGATCAC TCAATACCAG 721 TCAATTTGGA TAGTGATACA GATTCTTCAC CTCTACTGCT TGCAAAT Predicted gene structure (within gDNA segment 130489 to 126561): Exon 1 129889 129675 ( 215 n); cDNA 1 215 ( 215 n); score: 1.000 Intron 1 129674 128387 (1288 n); Pd: 0.865 (s: 1.00), Pa: 0.576 (s: 1.00) Exon 2 128386 128306 ( 81 n); cDNA 216 296 ( 81 n); score: 1.000 Intron 2 128305 127641 ( 665 n); Pd: 0.999 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 3 127640 127171 ( 470 n); cDNA 297 767 ( 471 n); score: 0.995 MATCH C08HBa0251H04.1- SGN-E318360+ 0.997 766 0.999 C PGS_C08HBa0251H04.1-_SGN-E318360+ (129889 129675,128386 128306,127640 127171) Alignment (genomic DNA sequence = upper lines): CACAACTCAT TGTTTTACCA ATTATAGGAC TATGGAAGCT GAAACATCCC AATTCTGAAT 129830 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACAACTCAT TGTTTTACCA ATTATAGGAC TATGGAAGCT GAAACATCCC AATTCTGAAT 60 TTGGTATTTT TTTTCTTCAT TAACCCTACT AACTTGGGGC TGTCATGGGG GAAATTTGCA 129770 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGGTATTTT TTTTCTTCAT TAACCCTACT AACTTGGGGC TGTCATGGGG GAAATTTGCA 120 TCCAATTTCT CTCTGATTTG CCCCAGGTGA AAGATGATTC ACAGCATTCT TGGCACATTG 129710 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCAATTTCT CTCTGATTTG CCCCAGGTGA AAGATGATTC ACAGCATTCT TGGCACATTG 180 TTTTCAGCTG CTACAAAACA GTCAATTTCA TTCAGGTACT AGTAACTTTG TATTTGTATA 129650 |||||||||| |||||||||| |||||||||| ||||| TTTTCAGCTG CTACAAAACA GTCAATTTCA TTCAG..... .......... .......... 215 TGTATTGCTC ATAGTGGGGT TGCTGATTAG CTGATAGAAA GTAGGGAAGA ATCATTGAAG 129590 .......... .......... .......... .......... .......... .......... 215 TCTTTGATGT TAATTGTGGG TCATTAAGAC AATTCAAGAA TGGACAATTT CTAGTTGGTT 129530 .......... .......... .......... .......... .......... .......... 215 TTAGGAAAAG GTTTTGATTT TTATGAATGT TGTTGAATTT TGACAAGTAT ATTTGTATTT 129470 .......... .......... .......... .......... .......... .......... 215 GTTTATGTAT TTCTCATAAT GGGGTTGCTG ATAAACTGAT AGAAAGTAGG GAAGAATCAT 129410 .......... .......... .......... .......... .......... .......... 215 TGAAGTCCTT GTTGTTAATT GTGGGTCATT AAGACAATTC AAGAATGGAC AATTTCAAGT 129350 .......... .......... .......... .......... .......... .......... 215 TGGTTTTAGG AAAAAGTTTT GATTTTTATG AATGTTGTGT TTTGACAAGT ATAGTTGTAT 129290 .......... .......... .......... .......... .......... .......... 215 TTCTCATAGT GGGATTTGCT GATAAACTGA TAGAAAGCAA GTAAAGAATC ATTGAAGACT 129230 .......... .......... .......... .......... .......... .......... 215 TAGTTGTTAA TTGTGAATCA TAAAGACATT CAAGAATGGC AATTTCAAGT TGGTTTGATG 129170 .......... .......... .......... .......... .......... .......... 215 AAATAGTTTT GATTTTTATG AATGTCGTTG TATTTTGACA AGTATATTTG TATTTCTTAT 129110 .......... .......... .......... .......... .......... .......... 215 AGTGGGGTTT CTGATAAACT GATAGAAAGC AAGTAAAGAA TCATTGAAGT CTTTTTTGTT 129050 .......... .......... .......... .......... .......... .......... 215 ATTTGTGGAT CATAAAGACA TTCAAGAATG GCAACTTCAA GTTGGTTTAA GGAGAAAAAT 128990 .......... .......... .......... .......... .......... .......... 215 TTTGATTTCT ATAAATGTTG TGCTTTGAGA AGTATATTGT GTATTTATGT CTGTACTTCT 128930 .......... .......... .......... .......... .......... .......... 215 CATAGTGGGG TTGCTGATAA ACTGATAGAA AACAAGCAAG AATCATTGAA GTTCTTATTG 128870 .......... .......... .......... .......... .......... .......... 215 TCCATTGTCG GTCATTGAGA GACAATTCAA GAATGGGCAA TTTCAAGTTG ATTTAAGGAA 128810 .......... .......... .......... .......... .......... .......... 215 AATGTTTTTA TTTTTATGAA TGTTGTTGTA TTTTGACAAC TATATTTGTA ATTTTATGTG 128750 .......... .......... .......... .......... .......... .......... 215 TACTACTCCT AGTTTATTGT CGATGAACTG TGAGAAAGCA AGAGAAGTAT TGAAATGGAC 128690 .......... .......... .......... .......... .......... .......... 215 AGTTTGAAGT TGGTTTTAGT AAAAAATTGA CTTTTTAGTT TTGCTCAAGC TTGCCCAGAC 128630 .......... .......... .......... .......... .......... .......... 215 ACCATGGGTA TCAAAAAAAA AAAAGATAGT GGCCTTTTAG TTCTTAGGGA TGTTTTTGTT 128570 .......... .......... .......... .......... .......... .......... 215 CGGTGAGAAG TATAGTTTTA GGATGGAGAA TTTCATTCTT GTATAGATTT TAGAGGGTTT 128510 .......... .......... .......... .......... .......... .......... 215 CATTTTTTTC CCCTGTAAAT CTTGATTTTG AAATTTGCAA TTGATGTTAC TGTTTGGGTG 128450 .......... .......... .......... .......... .......... .......... 215 TAATTTGTTA CTTCTTGATT GGCTATGTCC AGTTTGAAAT TTATAATTTG ACGTTATTGT 128390 .......... .......... .......... .......... .......... .......... 215 CAGGTGTAAT TTGTTACTAC TTGATTTGTT GTGTCCAATG GAACTAGTGG CTTGGGACTA 128330 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GTGTAAT TTGTTACTAC TTGATTTGTT GTGTCCAATG GAACTAGTGG CTTGGGACTA 272 GTAAGAGGAA GGAAGCTTTG ACAGGTAACG ACACATCCTT TGTTTCTGTT CTACAACTAA 128270 |||||||||| |||||||||| |||| GTAAGAGGAA GGAAGCTTTG ACAG...... .......... .......... .......... 296 TTTTAATGAA TTTGGTTAAA AATGCTTTGT CATTTATCTA TTAACTTTGT GCCGTCATAG 128210 .......... .......... .......... .......... .......... .......... 296 AAATATAGAA CGGCAGGATC ATAAGAGTAA CTGAAATCGC TTGAAACACT TTTAAATCCT 128150 .......... .......... .......... .......... .......... .......... 296 TTTCTCTCTA TATTGTTCTC CTAACATGGT AAGTGTTACC TTTAGAAATT CTATTTAAGA 128090 .......... .......... .......... .......... .......... .......... 296 CGGGGATTTT TTTCTGGAAA TTATGATGTT TATATAATAC TAGTAGCTTT ATTTATTATT 128030 .......... .......... .......... .......... .......... .......... 296 TCAGAGCTTT GACGTGCATT GATTGTGACG AATTGATCAT TTACAAAGTA TAATGCATAA 127970 .......... .......... .......... .......... .......... .......... 296 CATCAAGCAC ACTTAATAGC TAATAGGCAA TTGGTATTGC AATATCATTC AATGTGAAGG 127910 .......... .......... .......... .......... .......... .......... 296 AGGATAGCTC AATATTGTGT GCAGTTTTTG AATCCAACCT TACAAACTTA AACTATAGCA 127850 .......... .......... .......... .......... .......... .......... 296 TCTGGTATAA TTGGCTGAAG AACATCTTAG GCAGGACATG TACAGACATG CTTAAATGAA 127790 .......... .......... .......... .......... .......... .......... 296 CTTGCTGGAT GATTTACTTG GTTTTGTTAC TTTTTACACC AATTTGTTTG GTTTACTTTT 127730 .......... .......... .......... .......... .......... .......... 296 AAGTTTTAAT GATGTTTGTG TGCAAATAAA CTAGTTCTTT TTCACATAAT AATAATTTTA 127670 .......... .......... .......... .......... .......... .......... 296 GTAATAATGC AGTTACATCC ACATTGCAGT TGGCAATCTT CTAGCATTTT ATATTTTGGA 127610 | |||||||||| |||||||||| |||||||||| .......... .......... .........T TGGCAATCTT CTAGCATTTT ATATTTTGGA 327 GAACATTTTA AATCATTGTG ATAATATGGC TGGGGGAAGT CAACCACGCC AAAGTGACAA 127550 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAACATTTTA AATCATTGTG ATAATATGGC TGGGGGAAGT CAACCACGCC AAAGTGACAA 387 ACCTTCAATC AGATACAGGT GTAATCTACT TGATGGGGCA CTGTTTGAAA TCACTGATTT 127490 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCTTCAATC AGATACAGGT GTAATCTACT TGATGGGGCA CTGTTTGAAA TCACTGATTT 447 AGACCGACAG AGTAATGCCC CTATTTCTTC TGGAAATAAG GAGAACCCTA AAAATTCTGA 127430 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGACCGACAG AGTAATGCCC CTATTTCTTC TGGAAATAAG GAGAACCCTA AAAATTCTGA 507 CTCTGAATCT TCTAAGATAA TGAGCACAAG TGAGCTTATA TCAGCAGTTG GAAGTATATG 127370 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTCTGAATCT TCTAAGATAA TGAGCACAAG TGAGCTTATA TCAGCAGTTG GAAGTATATG 567 GTGTTCTGCA GCTGGCCCTG TTACACGAAT CCTATCTAAA GGAAGTTCTA GATGCAATAA 127310 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGTTCTGCA GCTGGCCCTG TTACACGAAT CCTATCTAAA GGAAGTTCTA GATGCAATAA 627 TACCGATCAC CAAGAAAACA ACATATTTGG TTACTCCACT GCAGATAAAA TTTC-TGGTG 127251 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| ||||| TACCGATCAC CAAGAAAACA ACATATTTGG TTACTCCACT GCAGATAAAA TTTCNTGGTG 687 TTTGTTTTTC ACCTCCTGAT CACTCAATAC CAGTCAATTT GGATAGTGAT ACAGATTCTT 127191 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTGTTTTTC ACCTCCTGAT CACTCAATAC CAGTCAATTT GGATAGTGAT ACAGATTCTT 747 CACCTCTACT GCTTGCAAAT 127171 |||||||||| |||||||||| CACCTCTACT GCTTGCAAAT 767 hqPGS_C08HBa0251H04.1-_SGN-E318360+ (129889 129675,128386 128306,127640 127171) ******************************************************************************** EST sequence 88 +strand 746 n (File: SGN-E327710+) 1 TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 61 ACAGTGGTAC AACTCAAAAA GATGTCTGTT GTAAATCAAC AATTTTTCAA CCCCATATGG 121 AAGCCATGTG TTTCTTCATG TCCATTGTTA GCTGCACAGT TTGTTGTGAT GCCAATGAGT 181 AATAGAGAGC TTTCTGTTGT TTCCACCATT GCTGCTGCAA AATCTACAGC TTCGATTGGT 241 GAAGGAAATG ATGATTATGT TGATATTAGT AGTGGGAAAG CAGCAGCCAA AGGGTCGGGT 301 ACTACGGCGA GAGGAAGGAG GTTGTTGAAG GTTAGGGAGG AGAAGAGGAA ACGGGAATAT 361 GAACGTCTTC ACAATTACCC TGCTTGGGCA AAAGTGTTGG AAGATGCCTG TAAACATGAT 421 ACAGAGCTTC GTGCTGTGCT TGGTGATAGC ATTGGAAATC CAGAGCTTAT GAGGAAAAAG 481 GTCGAAGAGA GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT 541 CTTGCATTCA AAGTCAGCTT TAGAGATTTT AATCCTCTTG ATTCATACAT ATGGTTTGAA 601 CTCTATGGCT CACCATCTGA TCGAGACGTT AACATTTTCG GTAGTGTTAT CCAGGCATGG 661 TACGTCATGG GACGTTTANG TGCATTTAAC TCATCGAATT TGCAGCTGGG GGGNTCATCA 721 ATGGAATTCA ATCCTCTCTA TGATGC Predicted gene structure (within gDNA segment 135496 to 131361): Exon 1 134896 134816 ( 81 n); cDNA 1 81 ( 81 n); score: 1.000 Intron 1 134815 134593 ( 223 n); Pd: 0.655 (s: 1.00), Pa: 0.777 (s: 1.00) Exon 2 134592 134282 ( 311 n); cDNA 82 392 ( 311 n); score: 1.000 Intron 2 134281 133706 ( 576 n); Pd: 0.996 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 3 133705 133618 ( 88 n); cDNA 393 480 ( 88 n); score: 1.000 Intron 3 133617 133467 ( 151 n); Pd: 0.987 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 4 133466 133381 ( 86 n); cDNA 481 566 ( 86 n); score: 1.000 Intron 4 133380 132643 ( 738 n); Pd: 0.895 (s: 1.00), Pa: 0.147 (s: 1.00) Exon 5 132642 132564 ( 79 n); cDNA 567 645 ( 79 n); score: 1.000 Intron 5 132563 132463 ( 101 n); Pd: 0.999 (s: 1.00), Pa: 1.000 (s: 0.98) Exon 6 132462 132403 ( 60 n); cDNA 646 705 ( 60 n); score: 0.983 Intron 6 132402 132012 ( 391 n); Pd: 0.999 (s: 0.98), Pa: 0.611 (s: 0.98) Exon 7 132011 131971 ( 41 n); cDNA 706 746 ( 41 n); score: 0.976 MATCH C08HBa0251H04.1- SGN-E327710+ 0.999 746 1.000 C PGS_C08HBa0251H04.1-_SGN-E327710+ (134896 134816,134592 134282,133705 133618,133466 133381,132642 132564,132462 132403,132011 131971) Alignment (genomic DNA sequence = upper lines): TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 134837 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 60 ACAGTGGTAC AACTCAAAAA GGTTATCAAG ATTACTTTTT TTTGAGTTCT GTGTTGAATT 134777 |||||||||| |||||||||| | ACAGTGGTAC AACTCAAAAA G......... .......... .......... .......... 81 TTTTAGCTTT GGTAGTTCTT TTTTTTTTTT TTTGTATGTA ATTGAGTTCT GTGTTGATTT 134717 .......... .......... .......... .......... .......... .......... 81 TCTGGGACTT TCTTGAATTT GGGTAAATGA AAAATTCATT TTTTTTCTCT GAATTCTGTG 134657 .......... .......... .......... .......... .......... .......... 81 TAAACCCAAA TTTGTAGGGT TTATGGTATG CTAAAACCCT TAATGCAATT CCATTTTTAT 134597 .......... .......... .......... .......... .......... .......... 81 GCAGATGTCT GTTGTAAATC AACAATTTTT CAACCCCATA TGGAAGCCAT GTGTTTCTTC 134537 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....ATGTCT GTTGTAAATC AACAATTTTT CAACCCCATA TGGAAGCCAT GTGTTTCTTC 137 ATGTCCATTG TTAGCTGCAC AGTTTGTTGT GATGCCAATG AGTAATAGAG AGCTTTCTGT 134477 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTCCATTG TTAGCTGCAC AGTTTGTTGT GATGCCAATG AGTAATAGAG AGCTTTCTGT 197 TGTTTCCACC ATTGCTGCTG CAAAATCTAC AGCTTCGATT GGTGAAGGAA ATGATGATTA 134417 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTTCCACC ATTGCTGCTG CAAAATCTAC AGCTTCGATT GGTGAAGGAA ATGATGATTA 257 TGTTGATATT AGTAGTGGGA AAGCAGCAGC CAAAGGGTCG GGTACTACGG CGAGAGGAAG 134357 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTGATATT AGTAGTGGGA AAGCAGCAGC CAAAGGGTCG GGTACTACGG CGAGAGGAAG 317 GAGGTTGTTG AAGGTTAGGG AGGAGAAGAG GAAACGGGAA TATGAACGTC TTCACAATTA 134297 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGGTTGTTG AAGGTTAGGG AGGAGAAGAG GAAACGGGAA TATGAACGTC TTCACAATTA 377 CCCTGCTTGG GCAAAGTATA TCTCTTAACT ACTTTGCCTA TTTGATGCTA TATTGTGTGT 134237 |||||||||| ||||| CCCTGCTTGG GCAAA..... .......... .......... .......... .......... 392 TTATATGTAT TTGTTCTGTC CCACACAAAT GGTTTACCTG TAACTAGTTG ATACCCCCAT 134177 .......... .......... .......... .......... .......... .......... 392 GAAAAAGTGA TCATATAGGA ATGCTTTAGA GGTAGAATAC ACTTTGAATT TTGATAAATG 134117 .......... .......... .......... .......... .......... .......... 392 CGTTGCTTGT GAAGTATGTG TCCTTGAGTG TCTTATAGAT TTACCCGCTA TTTGATGTTT 134057 .......... .......... .......... .......... .......... .......... 392 TATGTATTCG TTGTGTTAAA ATGGTATTTG ATACTTTCAT GATTACCATA TATATCTCAC 133997 .......... .......... .......... .......... .......... .......... 392 ACAAATCGTT TACTTGTAAC TAATTAGTAT CTCTAAGAAA AATAATCACA TAGGAATGCT 133937 .......... .......... .......... .......... .......... .......... 392 TGCGAGTCCG AATACTTTGA ATTTGAGAAA TGCATTGCTT GTGAAGTATG TGTGCGTGTA 133877 .......... .......... .......... .......... .......... .......... 392 TGACATATTG ATTTACCCTT TGTTAGTTGG AAGTTGGAAG CCAGACAAGC TAGAGTGCTA 133817 .......... .......... .......... .......... .......... .......... 392 GAGTCCATGC AACGTTGTTG CCATTAGGAT TCCAACATTG ATTTGATCTT CACTTTTACT 133757 .......... .......... .......... .......... .......... .......... 392 TCATCTGTGT CCTTGAGATT GACTTGTTTA TGTTAAAAAT GCTATTTTTA GAGTGTTGGA 133697 ||||||||| .......... .......... .......... .......... .......... .AGTGTTGGA 401 AGATGCCTGT AAACATGATA CAGAGCTTCG TGCTGTGCTT GGTGATAGCA TTGGAAATCC 133637 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATGCCTGT AAACATGATA CAGAGCTTCG TGCTGTGCTT GGTGATAGCA TTGGAAATCC 461 AGAGCTTATG AGGAAAAAGG TAGGTGTAAT TTTTATTTGG GATTTTGAGT TAGGTTTGAC 133577 |||||||||| ||||||||| AGAGCTTATG AGGAAAAAG. .......... .......... .......... .......... 480 AGACAGACAT TTGCAGATAC TCCACATTTT CGTTGATTTC AGTTCGCTTT AGATTTCGAT 133517 .......... .......... .......... .......... .......... .......... 480 TTATGACTTC CAATTGAAAT CTGCTTGTTT CCTTTTCTCC ATTTTGATAG GTCGAAGAGA 133457 |||||||||| .......... .......... .......... .......... .......... GTCGAAGAGA 490 GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT CTTGCATTCA 133397 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT CTTGCATTCA 550 AAGTCAGCTT TAGAGAGTAA TTAAATTCTG ACCAAACTTG ATGCTTCTTC TGTTTTCTCT 133337 |||||||||| |||||| AAGTCAGCTT TAGAGA.... .......... .......... .......... .......... 566 CCAAATTTTC TTTTCCTATC ACTATCTCCT AGATATATGA ATAGCATCCA GTGGCCAAAT 133277 .......... .......... .......... .......... .......... .......... 566 TCCTTCAAAA CATAATCAAG TGAAGTTTTT AAAGTTCCTT GGTAAAATGT GATTTTTTTT 133217 .......... .......... .......... .......... .......... .......... 566 TGTCTATTTG ATCTGTAGCT GTTTGTTCTG TTTAACTAGA GTAAATAAAT GAAGCAGAAT 133157 .......... .......... .......... .......... .......... .......... 566 TTTGGGAAGA ATAACCAGTC TAGGTCGTAG TGTTCTTTTT CATCGTCAGG AGTTCACTTC 133097 .......... .......... .......... .......... .......... .......... 566 TTATGTAGTC AGACTTGTGG CTGAGCTGCT AGAGCTTTTC CTGGGTTATT TTGTTTTAAA 133037 .......... .......... .......... .......... .......... .......... 566 ATAGAGGGGT TTTGTCAAAT TTTTGTTTGT TGGATGGGCT AATACTTTCT TTAAAATCTC 132977 .......... .......... .......... .......... .......... .......... 566 TATATGGTCT ATCAGGGTTC AGGTGTCGAG TTTCAGCACA AACTTTAGAA TACAGTTCAG 132917 .......... .......... .......... .......... .......... .......... 566 GAAGTGTCTG TCATGTTTGT TTGACTTGAC AATTATTCAA TACGATGTCT CCAATTTGTT 132857 .......... .......... .......... .......... .......... .......... 566 GTTCACATAT CATATTTACC GATATTAGTA AGTTGCATGT GGTTTGCAGA TCTTTATAGG 132797 .......... .......... .......... .......... .......... .......... 566 TCATATTGTT TTAATGCAAA TGTGTTTGGT ATGAAAGAAA ATGTTTTCTT GTTTTTAGTT 132737 .......... .......... .......... .......... .......... .......... 566 TGTGATGAAA TATATTTTGC AGAAAATTTC CTTCAAATTC CTTGCTATTT TGTTAAGATG 132677 .......... .......... .......... .......... .......... .......... 566 CATATCACTT ACAGCATTTT ATTTGAATCG ACAGTTTTAA TCCTCTTGAT TCATACATAT 132617 |||||| |||||||||| |||||||||| .......... .......... .......... ....TTTTAA TCCTCTTGAT TCATACATAT 592 GGTTTGAACT CTATGGCTCA CCATCTGATC GAGACGTTAA CATTTTCGGT AGTGTAAGTT 132557 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| GGTTTGAACT CTATGGCTCA CCATCTGATC GAGACGTTAA CATTTTCGGT AGT....... 645 TACTGCTTTC TTCTCTGTTC TCATATTACA TTACTTCATG CTTTTTAGTA TTTTGGGTTT 132497 .......... .......... .......... .......... .......... .......... 645 CATAACTTGT AATATCTTGT GCTTCTATTG GCAGGTTATC CAGGCATGGT ACGTCATGGG 132437 |||||| |||||||||| |||||||||| .......... .......... .......... ....GTTATC CAGGCATGGT ACGTCATGGG 671 ACGTTTAGGT GCATTTAACT CATCGAATTT GCAGGTGAGT TCACTGCTCA ACATGGATTT 132377 ||||||| || |||||||||| |||||||||| |||| ACGTTTANGT GCATTTAACT CATCGAATTT GCAG...... .......... .......... 705 TATTTAAAGT TTCTTTTTTT GCTGTTGTTA AGTGTTGTTC TTGCCATCGC TCTACTTCAG 132317 .......... .......... .......... .......... .......... .......... 705 CAAATAACTA AACATTAAGT TTAGTACGTA CAATCACATG CGTTTGAAAG TTTTCCTACT 132257 .......... .......... .......... .......... .......... .......... 705 TTAAATCACG ATGGTGTGCG GTCAGGTTGT AATTGTAGTA AGTTGTTGGT TCTATTCTTG 132197 .......... .......... .......... .......... .......... .......... 705 ATCATTTCAT TCATTCATCC CCCTTTTATT ATCATACATT ACGAAGAATA GGAAGTTCCT 132137 .......... .......... .......... .......... .......... .......... 705 CCACGTGATC GGAAAAAGGT ATCATTTCAT TCATTCATCC CCCTTTTATT ATCGTACATT 132077 .......... .......... .......... .......... .......... .......... 705 ACGAAGAATA GGAAGTTCCT CCACGTGATC GGAAAAAGGT TCAAGTTTGT GTTAAATGAA 132017 .......... .......... .......... .......... .......... .......... 705 TGCAGCTGGG GGGTTCATCA ATGGAATTCA ATCCTCTCTA TGATGC 131971 ||||| ||| |||||| |||||||||| |||||||||| |||||| .....CTGGG GGGNTCATCA ATGGAATTCA ATCCTCTCTA TGATGC 746 hqPGS_C08HBa0251H04.1-_SGN-E327710+ (134896 134816,134592 134282,133705 133618,133466 133381,132642 132564,132462 132403,132011 131971) ******************************************************************************** EST sequence 119 +strand 596 n (File: SGN-E332397+) 1 TTTTTAGAGA GATTTTAGCT CAACAACTCA TGTTAAACAG TGGTACAACT CAAAAAGATG 61 TCTGTTGTAA ATCAACAATT TTTCAACCCC ATATGGAAGC CATGTGTTTC TTCATGTCCA 121 TTGTTAGCTG CACAGTTTGT TGTGATGCCA ATGAGTAATA GAGAGCTTTC TGTTGTTTCC 181 ACCATTGCTG CTGCAAAATC TACAGCTTCG ATTGGTGAAG GAAATGATGA TTATGTTGAT 241 ATTAGTAGTG GGAAAGCAGC AGCCAAAGGG TCGGGTACTA CGGCGAGAGG AAGGAGGTTG 301 TTGAAGGTTA GGGAGGAGAA GAGGAAACGG GAATATGAAC GTCTTCACAA TTACCCTGCT 361 TGGGCAAAAG TGTTGGAAGA TGCCTGTAAA CATGATACAG AGCTTCGTGC TGTGCTTGGT 421 GATAGCATTG GAAATCCAGA GCTTATGAGG AAAAAGGTCG AAGAGAGAGT TCGAACAAAG 481 GGCCGAAATT TTCAGAAGTC TAAAACAGGA TCAGTTCTTG CATTCAAAGT CAGCTTTAGA 541 AATTTAATCC TCTTGATTCA TACATATGGT TTGAACTCTA TGGCTCACCA TCTGAT Predicted gene structure (within gDNA segment 135472 to 131978): Exon 1 134872 134816 ( 57 n); cDNA 1 57 ( 57 n); score: 1.000 Intron 1 134815 134593 ( 223 n); Pd: 0.655 (s: 1.00), Pa: 0.777 (s: 1.00) Exon 2 134592 134282 ( 311 n); cDNA 58 368 ( 311 n); score: 1.000 Intron 2 134281 133706 ( 576 n); Pd: 0.996 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 3 133705 133618 ( 88 n); cDNA 369 456 ( 88 n); score: 1.000 Intron 3 133617 133467 ( 151 n); Pd: 0.987 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 4 133466 133381 ( 86 n); cDNA 457 541 ( 85 n); score: 0.988 Intron 4 133380 132643 ( 738 n); Pd: 0.895 (s: 0.98), Pa: 0.147 (s: 0.98) Exon 5 132642 132588 ( 55 n); cDNA 542 596 ( 55 n); score: 0.982 MATCH C08HBa0251H04.1- SGN-E332397+ 0.997 597 1.002 C PGS_C08HBa0251H04.1-_SGN-E332397+ (134872 134816,134592 134282,133705 133618,133466 133381,132642 132588) Alignment (genomic DNA sequence = upper lines): TTTTTAGAGA GATTTTAGCT CAACAACTCA TGTTAAACAG TGGTACAACT CAAAAAGGTT 134813 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| TTTTTAGAGA GATTTTAGCT CAACAACTCA TGTTAAACAG TGGTACAACT CAAAAAG... 57 ATCAAGATTA CTTTTTTTTG AGTTCTGTGT TGAATTTTTT AGCTTTGGTA GTTCTTTTTT 134753 .......... .......... .......... .......... .......... .......... 57 TTTTTTTTTG TATGTAATTG AGTTCTGTGT TGATTTTCTG GGACTTTCTT GAATTTGGGT 134693 .......... .......... .......... .......... .......... .......... 57 AAATGAAAAA TTCATTTTTT TTCTCTGAAT TCTGTGTAAA CCCAAATTTG TAGGGTTTAT 134633 .......... .......... .......... .......... .......... .......... 57 GGTATGCTAA AACCCTTAAT GCAATTCCAT TTTTATGCAG ATGTCTGTTG TAAATCAACA 134573 |||||||||| |||||||||| .......... .......... .......... .......... ATGTCTGTTG TAAATCAACA 77 ATTTTTCAAC CCCATATGGA AGCCATGTGT TTCTTCATGT CCATTGTTAG CTGCACAGTT 134513 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTTTCAAC CCCATATGGA AGCCATGTGT TTCTTCATGT CCATTGTTAG CTGCACAGTT 137 TGTTGTGATG CCAATGAGTA ATAGAGAGCT TTCTGTTGTT TCCACCATTG CTGCTGCAAA 134453 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTGTGATG CCAATGAGTA ATAGAGAGCT TTCTGTTGTT TCCACCATTG CTGCTGCAAA 197 ATCTACAGCT TCGATTGGTG AAGGAAATGA TGATTATGTT GATATTAGTA GTGGGAAAGC 134393 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCTACAGCT TCGATTGGTG AAGGAAATGA TGATTATGTT GATATTAGTA GTGGGAAAGC 257 AGCAGCCAAA GGGTCGGGTA CTACGGCGAG AGGAAGGAGG TTGTTGAAGG TTAGGGAGGA 134333 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCAGCCAAA GGGTCGGGTA CTACGGCGAG AGGAAGGAGG TTGTTGAAGG TTAGGGAGGA 317 GAAGAGGAAA CGGGAATATG AACGTCTTCA CAATTACCCT GCTTGGGCAA AGTATATCTC 134273 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | GAAGAGGAAA CGGGAATATG AACGTCTTCA CAATTACCCT GCTTGGGCAA A......... 368 TTAACTACTT TGCCTATTTG ATGCTATATT GTGTGTTTAT ATGTATTTGT TCTGTCCCAC 134213 .......... .......... .......... .......... .......... .......... 368 ACAAATGGTT TACCTGTAAC TAGTTGATAC CCCCATGAAA AAGTGATCAT ATAGGAATGC 134153 .......... .......... .......... .......... .......... .......... 368 TTTAGAGGTA GAATACACTT TGAATTTTGA TAAATGCGTT GCTTGTGAAG TATGTGTCCT 134093 .......... .......... .......... .......... .......... .......... 368 TGAGTGTCTT ATAGATTTAC CCGCTATTTG ATGTTTTATG TATTCGTTGT GTTAAAATGG 134033 .......... .......... .......... .......... .......... .......... 368 TATTTGATAC TTTCATGATT ACCATATATA TCTCACACAA ATCGTTTACT TGTAACTAAT 133973 .......... .......... .......... .......... .......... .......... 368 TAGTATCTCT AAGAAAAATA ATCACATAGG AATGCTTGCG AGTCCGAATA CTTTGAATTT 133913 .......... .......... .......... .......... .......... .......... 368 GAGAAATGCA TTGCTTGTGA AGTATGTGTG CGTGTATGAC ATATTGATTT ACCCTTTGTT 133853 .......... .......... .......... .......... .......... .......... 368 AGTTGGAAGT TGGAAGCCAG ACAAGCTAGA GTGCTAGAGT CCATGCAACG TTGTTGCCAT 133793 .......... .......... .......... .......... .......... .......... 368 TAGGATTCCA ACATTGATTT GATCTTCACT TTTACTTCAT CTGTGTCCTT GAGATTGACT 133733 .......... .......... .......... .......... .......... .......... 368 TGTTTATGTT AAAAATGCTA TTTTTAGAGT GTTGGAAGAT GCCTGTAAAC ATGATACAGA 133673 ||| |||||||||| |||||||||| |||||||||| .......... .......... .......AGT GTTGGAAGAT GCCTGTAAAC ATGATACAGA 401 GCTTCGTGCT GTGCTTGGTG ATAGCATTGG AAATCCAGAG CTTATGAGGA AAAAGGTAGG 133613 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| GCTTCGTGCT GTGCTTGGTG ATAGCATTGG AAATCCAGAG CTTATGAGGA AAAAG..... 456 TGTAATTTTT ATTTGGGATT TTGAGTTAGG TTTGACAGAC AGACATTTGC AGATACTCCA 133553 .......... .......... .......... .......... .......... .......... 456 CATTTTCGTT GATTTCAGTT CGCTTTAGAT TTCGATTTAT GACTTCCAAT TGAAATCTGC 133493 .......... .......... .......... .......... .......... .......... 456 TTGTTTCCTT TTCTCCATTT TGATAGGTCG AAGAGAGAGT TCGAACAAAG GGCCGAAATT 133433 |||| |||||||||| |||||||||| |||||||||| .......... .......... ......GTCG AAGAGAGAGT TCGAACAAAG GGCCGAAATT 490 TTCAGAAGTC TAAAACAGGA TCAGTTCTTG CATTCAAAGT CAGCTTTAGA GAGTAATTAA 133373 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| | TTCAGAAGTC TAAAACAGGA TCAGTTCTTG CATTCAAAGT CAGCTTTAGA -A........ 541 ATTCTGACCA AACTTGATGC TTCTTCTGTT TTCTCTCCAA ATTTTCTTTT CCTATCACTA 133313 .......... .......... .......... .......... .......... .......... 541 TCTCCTAGAT ATATGAATAG CATCCAGTGG CCAAATTCCT TCAAAACATA ATCAAGTGAA 133253 .......... .......... .......... .......... .......... .......... 541 GTTTTTAAAG TTCCTTGGTA AAATGTGATT TTTTTTTGTC TATTTGATCT GTAGCTGTTT 133193 .......... .......... .......... .......... .......... .......... 541 GTTCTGTTTA ACTAGAGTAA ATAAATGAAG CAGAATTTTG GGAAGAATAA CCAGTCTAGG 133133 .......... .......... .......... .......... .......... .......... 541 TCGTAGTGTT CTTTTTCATC GTCAGGAGTT CACTTCTTAT GTAGTCAGAC TTGTGGCTGA 133073 .......... .......... .......... .......... .......... .......... 541 GCTGCTAGAG CTTTTCCTGG GTTATTTTGT TTTAAAATAG AGGGGTTTTG TCAAATTTTT 133013 .......... .......... .......... .......... .......... .......... 541 GTTTGTTGGA TGGGCTAATA CTTTCTTTAA AATCTCTATA TGGTCTATCA GGGTTCAGGT 132953 .......... .......... .......... .......... .......... .......... 541 GTCGAGTTTC AGCACAAACT TTAGAATACA GTTCAGGAAG TGTCTGTCAT GTTTGTTTGA 132893 .......... .......... .......... .......... .......... .......... 541 CTTGACAATT ATTCAATACG ATGTCTCCAA TTTGTTGTTC ACATATCATA TTTACCGATA 132833 .......... .......... .......... .......... .......... .......... 541 TTAGTAAGTT GCATGTGGTT TGCAGATCTT TATAGGTCAT ATTGTTTTAA TGCAAATGTG 132773 .......... .......... .......... .......... .......... .......... 541 TTTGGTATGA AAGAAAATGT TTTCTTGTTT TTAGTTTGTG ATGAAATATA TTTTGCAGAA 132713 .......... .......... .......... .......... .......... .......... 541 AATTTCCTTC AAATTCCTTG CTATTTTGTT AAGATGCATA TCACTTACAG CATTTTATTT 132653 .......... .......... .......... .......... .......... .......... 541 GAATCGACAG TTTTAATCCT CTTGATTCAT ACATATGGTT TGAACTCTAT GGCTCACCAT 132593 ||||||||| |||||||||| |||||||||| |||||||||| |||||||||| .......... ATTTAATCCT CTTGATTCAT ACATATGGTT TGAACTCTAT GGCTCACCAT 591 CTGAT 132588 ||||| CTGAT 596 hqPGS_C08HBa0251H04.1-_SGN-E332397+ (134872 134816,134592 134282,133705 133618,133466 133381,132642 132588) ******************************************************************************** EST sequence 131 +strand 595 n (File: SGN-E329166+) 1 TTTAGAGAGA TTTTATCTCA ACAACTCATG TTAAACAGTG GTACAACTCA AAAAGATGGC 61 TGTTGTAAAT CAACAATTTT TCAACCCCAT ATGGAAGCCA TGTGTTTCTT CATGTCCATT 121 GTTAGCTGCA CAGTTTGTTG TGATGCCAAT GAGTAATAGA GAGCTTTCTG TTGTTTCCAC 181 CATTGCTGCT GCAAAATCTA CAGCTTCGAT TGGTGAAGGA AATGATGATT ATGTTGATAT 241 TAGTAGTGGG AAAGCAGCAG CCAAAGGGTC GGGTACTACG GCGAGAGGAA AGAGGTTGTT 301 GAAGGTTAGG GAGGAGAAGA GGAAACGGGA ATATGAACGT CTTCACAATT ACCCTGCTTG 361 GGCAAAAGTG TTGGAAGATG CCTGTAAACA TGATACAGAG CTTCGTGCTG TGCTTGGTGA 421 TAGCATTGGA AATCCAGAGC TTATGAGGAA AAAGGTCGAA GAGAGAGTTC GAACAAAGGG 481 CCGAAATTTT CAGAAGTCTA AAACAGGATC AGTTCTTGCA TTCAAAGTCA GCTTTAGAGA 541 TTTTAATCCT CTTGATTCAT ACATATGGTT TGAACTCTAT GGCTCACCAT CTGAT Predicted gene structure (within gDNA segment 135614 to 131978): Exon 1 134870 134816 ( 55 n); cDNA 1 55 ( 55 n); score: 0.982 Intron 1 134815 134593 ( 223 n); Pd: 0.655 (s: 0.98), Pa: 0.777 (s: 0.98) Exon 2 134592 134282 ( 311 n); cDNA 56 366 ( 311 n); score: 0.994 Intron 2 134281 133706 ( 576 n); Pd: 0.996 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 3 133705 133618 ( 88 n); cDNA 367 454 ( 88 n); score: 1.000 Intron 3 133617 133467 ( 151 n); Pd: 0.987 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 4 133466 133381 ( 86 n); cDNA 455 540 ( 86 n); score: 1.000 Intron 4 133380 132643 ( 738 n); Pd: 0.895 (s: 1.00), Pa: 0.147 (s: 1.00) Exon 5 132642 132588 ( 55 n); cDNA 541 595 ( 55 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E329166+ 0.995 595 1.000 C PGS_C08HBa0251H04.1-_SGN-E329166+ (134870 134816,134592 134282,133705 133618,133466 133381,132642 132588) Alignment (genomic DNA sequence = upper lines): TTTAGAGAGA TTTTAGCTCA ACAACTCATG TTAAACAGTG GTACAACTCA AAAAGGTTAT 134811 |||||||||| ||||| |||| |||||||||| |||||||||| |||||||||| ||||| TTTAGAGAGA TTTTATCTCA ACAACTCATG TTAAACAGTG GTACAACTCA AAAAG..... 55 CAAGATTACT TTTTTTTGAG TTCTGTGTTG AATTTTTTAG CTTTGGTAGT TCTTTTTTTT 134751 .......... .......... .......... .......... .......... .......... 55 TTTTTTTGTA TGTAATTGAG TTCTGTGTTG ATTTTCTGGG ACTTTCTTGA ATTTGGGTAA 134691 .......... .......... .......... .......... .......... .......... 55 ATGAAAAATT CATTTTTTTT CTCTGAATTC TGTGTAAACC CAAATTTGTA GGGTTTATGG 134631 .......... .......... .......... .......... .......... .......... 55 TATGCTAAAA CCCTTAATGC AATTCCATTT TTATGCAGAT GTCTGTTGTA AATCAACAAT 134571 || | |||||||| |||||||||| .......... .......... .......... ........AT GGCTGTTGTA AATCAACAAT 77 TTTTCAACCC CATATGGAAG CCATGTGTTT CTTCATGTCC ATTGTTAGCT GCACAGTTTG 134511 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTCAACCC CATATGGAAG CCATGTGTTT CTTCATGTCC ATTGTTAGCT GCACAGTTTG 137 TTGTGATGCC AATGAGTAAT AGAGAGCTTT CTGTTGTTTC CACCATTGCT GCTGCAAAAT 134451 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTGATGCC AATGAGTAAT AGAGAGCTTT CTGTTGTTTC CACCATTGCT GCTGCAAAAT 197 CTACAGCTTC GATTGGTGAA GGAAATGATG ATTATGTTGA TATTAGTAGT GGGAAAGCAG 134391 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTACAGCTTC GATTGGTGAA GGAAATGATG ATTATGTTGA TATTAGTAGT GGGAAAGCAG 257 CAGCCAAAGG GTCGGGTACT ACGGCGAGAG GAAGGAGGTT GTTGAAGGTT AGGGAGGAGA 134331 |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| |||||||||| CAGCCAAAGG GTCGGGTACT ACGGCGAGAG GAAAGAGGTT GTTGAAGGTT AGGGAGGAGA 317 AGAGGAAACG GGAATATGAA CGTCTTCACA ATTACCCTGC TTGGGCAAAG TATATCTCTT 134271 |||||||||| |||||||||| |||||||||| |||||||||| ||||||||| AGAGGAAACG GGAATATGAA CGTCTTCACA ATTACCCTGC TTGGGCAAA. .......... 366 AACTACTTTG CCTATTTGAT GCTATATTGT GTGTTTATAT GTATTTGTTC TGTCCCACAC 134211 .......... .......... .......... .......... .......... .......... 366 AAATGGTTTA CCTGTAACTA GTTGATACCC CCATGAAAAA GTGATCATAT AGGAATGCTT 134151 .......... .......... .......... .......... .......... .......... 366 TAGAGGTAGA ATACACTTTG AATTTTGATA AATGCGTTGC TTGTGAAGTA TGTGTCCTTG 134091 .......... .......... .......... .......... .......... .......... 366 AGTGTCTTAT AGATTTACCC GCTATTTGAT GTTTTATGTA TTCGTTGTGT TAAAATGGTA 134031 .......... .......... .......... .......... .......... .......... 366 TTTGATACTT TCATGATTAC CATATATATC TCACACAAAT CGTTTACTTG TAACTAATTA 133971 .......... .......... .......... .......... .......... .......... 366 GTATCTCTAA GAAAAATAAT CACATAGGAA TGCTTGCGAG TCCGAATACT TTGAATTTGA 133911 .......... .......... .......... .......... .......... .......... 366 GAAATGCATT GCTTGTGAAG TATGTGTGCG TGTATGACAT ATTGATTTAC CCTTTGTTAG 133851 .......... .......... .......... .......... .......... .......... 366 TTGGAAGTTG GAAGCCAGAC AAGCTAGAGT GCTAGAGTCC ATGCAACGTT GTTGCCATTA 133791 .......... .......... .......... .......... .......... .......... 366 GGATTCCAAC ATTGATTTGA TCTTCACTTT TACTTCATCT GTGTCCTTGA GATTGACTTG 133731 .......... .......... .......... .......... .......... .......... 366 TTTATGTTAA AAATGCTATT TTTAGAGTGT TGGAAGATGC CTGTAAACAT GATACAGAGC 133671 ||||| |||||||||| |||||||||| |||||||||| .......... .......... .....AGTGT TGGAAGATGC CTGTAAACAT GATACAGAGC 401 TTCGTGCTGT GCTTGGTGAT AGCATTGGAA ATCCAGAGCT TATGAGGAAA AAGGTAGGTG 133611 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| TTCGTGCTGT GCTTGGTGAT AGCATTGGAA ATCCAGAGCT TATGAGGAAA AAG....... 454 TAATTTTTAT TTGGGATTTT GAGTTAGGTT TGACAGACAG ACATTTGCAG ATACTCCACA 133551 .......... .......... .......... .......... .......... .......... 454 TTTTCGTTGA TTTCAGTTCG CTTTAGATTT CGATTTATGA CTTCCAATTG AAATCTGCTT 133491 .......... .......... .......... .......... .......... .......... 454 GTTTCCTTTT CTCCATTTTG ATAGGTCGAA GAGAGAGTTC GAACAAAGGG CCGAAATTTT 133431 |||||| |||||||||| |||||||||| |||||||||| .......... .......... ....GTCGAA GAGAGAGTTC GAACAAAGGG CCGAAATTTT 490 CAGAAGTCTA AAACAGGATC AGTTCTTGCA TTCAAAGTCA GCTTTAGAGA GTAATTAAAT 133371 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGAAGTCTA AAACAGGATC AGTTCTTGCA TTCAAAGTCA GCTTTAGAGA .......... 540 TCTGACCAAA CTTGATGCTT CTTCTGTTTT CTCTCCAAAT TTTCTTTTCC TATCACTATC 133311 .......... .......... .......... .......... .......... .......... 540 TCCTAGATAT ATGAATAGCA TCCAGTGGCC AAATTCCTTC AAAACATAAT CAAGTGAAGT 133251 .......... .......... .......... .......... .......... .......... 540 TTTTAAAGTT CCTTGGTAAA ATGTGATTTT TTTTTGTCTA TTTGATCTGT AGCTGTTTGT 133191 .......... .......... .......... .......... .......... .......... 540 TCTGTTTAAC TAGAGTAAAT AAATGAAGCA GAATTTTGGG AAGAATAACC AGTCTAGGTC 133131 .......... .......... .......... .......... .......... .......... 540 GTAGTGTTCT TTTTCATCGT CAGGAGTTCA CTTCTTATGT AGTCAGACTT GTGGCTGAGC 133071 .......... .......... .......... .......... .......... .......... 540 TGCTAGAGCT TTTCCTGGGT TATTTTGTTT TAAAATAGAG GGGTTTTGTC AAATTTTTGT 133011 .......... .......... .......... .......... .......... .......... 540 TTGTTGGATG GGCTAATACT TTCTTTAAAA TCTCTATATG GTCTATCAGG GTTCAGGTGT 132951 .......... .......... .......... .......... .......... .......... 540 CGAGTTTCAG CACAAACTTT AGAATACAGT TCAGGAAGTG TCTGTCATGT TTGTTTGACT 132891 .......... .......... .......... .......... .......... .......... 540 TGACAATTAT TCAATACGAT GTCTCCAATT TGTTGTTCAC ATATCATATT TACCGATATT 132831 .......... .......... .......... .......... .......... .......... 540 AGTAAGTTGC ATGTGGTTTG CAGATCTTTA TAGGTCATAT TGTTTTAATG CAAATGTGTT 132771 .......... .......... .......... .......... .......... .......... 540 TGGTATGAAA GAAAATGTTT TCTTGTTTTT AGTTTGTGAT GAAATATATT TTGCAGAAAA 132711 .......... .......... .......... .......... .......... .......... 540 TTTCCTTCAA ATTCCTTGCT ATTTTGTTAA GATGCATATC ACTTACAGCA TTTTATTTGA 132651 .......... .......... .......... .......... .......... .......... 540 ATCGACAGTT TTAATCCTCT TGATTCATAC ATATGGTTTG AACTCTATGG CTCACCATCT 132591 || |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ........TT TTAATCCTCT TGATTCATAC ATATGGTTTG AACTCTATGG CTCACCATCT 592 GAT 132588 ||| GAT 595 hqPGS_C08HBa0251H04.1-_SGN-E329166+ (134870 134816,134592 134282,133705 133618,133466 133381,132642 132588) ******************************************************************************** EST sequence 94 +strand 607 n (File: SGN-E328376+) 1 TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 61 ACAGTGGTAC AACTCAAAAA GATGTCTGTT GTAAATCAAC AATTTTTCAA CCCCATATGG 121 AAGCCATGTG TTTCTTCATG TCCATTGTTA GCTGCACAGT TTGTTGTGAT GCCAATGAGT 181 AATAGAGAGC TTTCTGTTGT TTCCACCATT GCTGCTGCAA AATCTACAGC TTCGATTGGT 241 GAAGGAAATG ATGATTATGT TGATATTAGT AGTGGGAAAG CAGCAGCCAA AGGGTCGGGT 301 ACTACGGCGA GAGGAAGGAG GTTGTTGAAG GTTAGGGAGG AGAAGAGGAA ACGGGAATAT 361 GAACGTCTTC ACAATTACCC TGCTTGGGCA AAAGTGTTGG AAGATGCCTG TAAACATGAT 421 ACAGAGCTTC GTGCTGTGCT TGGTGATAGC ATTGGAAATC CAGAGCTTAT GAGGAAAAAA 481 GTCGAAGAGA GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT 541 CTTGCATTTC AAGTCAGCTT TAGAGATTTT AATCCTCTTG ATTCATACAT ATGGTTTGAA 601 CTCTATG Predicted gene structure (within gDNA segment 135496 to 131992): Exon 1 134896 134816 ( 81 n); cDNA 1 81 ( 81 n); score: 1.000 Intron 1 134815 134593 ( 223 n); Pd: 0.655 (s: 1.00), Pa: 0.777 (s: 1.00) Exon 2 134592 134282 ( 311 n); cDNA 82 392 ( 311 n); score: 1.000 Intron 2 134281 133706 ( 576 n); Pd: 0.996 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 3 133705 133618 ( 88 n); cDNA 393 480 ( 88 n); score: 0.989 Intron 3 133617 133467 ( 151 n); Pd: 0.987 (s: 0.98), Pa: 0.991 (s: 1.00) Exon 4 133466 133381 ( 86 n); cDNA 481 566 ( 86 n); score: 0.977 Intron 4 133380 132643 ( 738 n); Pd: 0.895 (s: 0.96), Pa: 0.147 (s: 1.00) Exon 5 132642 132602 ( 41 n); cDNA 567 607 ( 41 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E328376+ 0.995 607 1.000 C PGS_C08HBa0251H04.1-_SGN-E328376+ (134896 134816,134592 134282,133705 133618,133466 133381,132642 132602) Alignment (genomic DNA sequence = upper lines): TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 134837 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTTTCAATC CTCCAAAGTT GCAGTTTTTA GAGAGATTTT AGCTCAACAA CTCATGTTAA 60 ACAGTGGTAC AACTCAAAAA GGTTATCAAG ATTACTTTTT TTTGAGTTCT GTGTTGAATT 134777 |||||||||| |||||||||| | ACAGTGGTAC AACTCAAAAA G......... .......... .......... .......... 81 TTTTAGCTTT GGTAGTTCTT TTTTTTTTTT TTTGTATGTA ATTGAGTTCT GTGTTGATTT 134717 .......... .......... .......... .......... .......... .......... 81 TCTGGGACTT TCTTGAATTT GGGTAAATGA AAAATTCATT TTTTTTCTCT GAATTCTGTG 134657 .......... .......... .......... .......... .......... .......... 81 TAAACCCAAA TTTGTAGGGT TTATGGTATG CTAAAACCCT TAATGCAATT CCATTTTTAT 134597 .......... .......... .......... .......... .......... .......... 81 GCAGATGTCT GTTGTAAATC AACAATTTTT CAACCCCATA TGGAAGCCAT GTGTTTCTTC 134537 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....ATGTCT GTTGTAAATC AACAATTTTT CAACCCCATA TGGAAGCCAT GTGTTTCTTC 137 ATGTCCATTG TTAGCTGCAC AGTTTGTTGT GATGCCAATG AGTAATAGAG AGCTTTCTGT 134477 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGTCCATTG TTAGCTGCAC AGTTTGTTGT GATGCCAATG AGTAATAGAG AGCTTTCTGT 197 TGTTTCCACC ATTGCTGCTG CAAAATCTAC AGCTTCGATT GGTGAAGGAA ATGATGATTA 134417 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTTCCACC ATTGCTGCTG CAAAATCTAC AGCTTCGATT GGTGAAGGAA ATGATGATTA 257 TGTTGATATT AGTAGTGGGA AAGCAGCAGC CAAAGGGTCG GGTACTACGG CGAGAGGAAG 134357 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTTGATATT AGTAGTGGGA AAGCAGCAGC CAAAGGGTCG GGTACTACGG CGAGAGGAAG 317 GAGGTTGTTG AAGGTTAGGG AGGAGAAGAG GAAACGGGAA TATGAACGTC TTCACAATTA 134297 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGGTTGTTG AAGGTTAGGG AGGAGAAGAG GAAACGGGAA TATGAACGTC TTCACAATTA 377 CCCTGCTTGG GCAAAGTATA TCTCTTAACT ACTTTGCCTA TTTGATGCTA TATTGTGTGT 134237 |||||||||| ||||| CCCTGCTTGG GCAAA..... .......... .......... .......... .......... 392 TTATATGTAT TTGTTCTGTC CCACACAAAT GGTTTACCTG TAACTAGTTG ATACCCCCAT 134177 .......... .......... .......... .......... .......... .......... 392 GAAAAAGTGA TCATATAGGA ATGCTTTAGA GGTAGAATAC ACTTTGAATT TTGATAAATG 134117 .......... .......... .......... .......... .......... .......... 392 CGTTGCTTGT GAAGTATGTG TCCTTGAGTG TCTTATAGAT TTACCCGCTA TTTGATGTTT 134057 .......... .......... .......... .......... .......... .......... 392 TATGTATTCG TTGTGTTAAA ATGGTATTTG ATACTTTCAT GATTACCATA TATATCTCAC 133997 .......... .......... .......... .......... .......... .......... 392 ACAAATCGTT TACTTGTAAC TAATTAGTAT CTCTAAGAAA AATAATCACA TAGGAATGCT 133937 .......... .......... .......... .......... .......... .......... 392 TGCGAGTCCG AATACTTTGA ATTTGAGAAA TGCATTGCTT GTGAAGTATG TGTGCGTGTA 133877 .......... .......... .......... .......... .......... .......... 392 TGACATATTG ATTTACCCTT TGTTAGTTGG AAGTTGGAAG CCAGACAAGC TAGAGTGCTA 133817 .......... .......... .......... .......... .......... .......... 392 GAGTCCATGC AACGTTGTTG CCATTAGGAT TCCAACATTG ATTTGATCTT CACTTTTACT 133757 .......... .......... .......... .......... .......... .......... 392 TCATCTGTGT CCTTGAGATT GACTTGTTTA TGTTAAAAAT GCTATTTTTA GAGTGTTGGA 133697 ||||||||| .......... .......... .......... .......... .......... .AGTGTTGGA 401 AGATGCCTGT AAACATGATA CAGAGCTTCG TGCTGTGCTT GGTGATAGCA TTGGAAATCC 133637 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATGCCTGT AAACATGATA CAGAGCTTCG TGCTGTGCTT GGTGATAGCA TTGGAAATCC 461 AGAGCTTATG AGGAAAAAGG TAGGTGTAAT TTTTATTTGG GATTTTGAGT TAGGTTTGAC 133577 |||||||||| |||||||| AGAGCTTATG AGGAAAAAA. .......... .......... .......... .......... 480 AGACAGACAT TTGCAGATAC TCCACATTTT CGTTGATTTC AGTTCGCTTT AGATTTCGAT 133517 .......... .......... .......... .......... .......... .......... 480 TTATGACTTC CAATTGAAAT CTGCTTGTTT CCTTTTCTCC ATTTTGATAG GTCGAAGAGA 133457 |||||||||| .......... .......... .......... .......... .......... GTCGAAGAGA 490 GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT CTTGCATTCA 133397 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||| GAGTTCGAAC AAAGGGCCGA AATTTTCAGA AGTCTAAAAC AGGATCAGTT CTTGCATTTC 550 AAGTCAGCTT TAGAGAGTAA TTAAATTCTG ACCAAACTTG ATGCTTCTTC TGTTTTCTCT 133337 |||||||||| |||||| AAGTCAGCTT TAGAGA.... .......... .......... .......... .......... 566 CCAAATTTTC TTTTCCTATC ACTATCTCCT AGATATATGA ATAGCATCCA GTGGCCAAAT 133277 .......... .......... .......... .......... .......... .......... 566 TCCTTCAAAA CATAATCAAG TGAAGTTTTT AAAGTTCCTT GGTAAAATGT GATTTTTTTT 133217 .......... .......... .......... .......... .......... .......... 566 TGTCTATTTG ATCTGTAGCT GTTTGTTCTG TTTAACTAGA GTAAATAAAT GAAGCAGAAT 133157 .......... .......... .......... .......... .......... .......... 566 TTTGGGAAGA ATAACCAGTC TAGGTCGTAG TGTTCTTTTT CATCGTCAGG AGTTCACTTC 133097 .......... .......... .......... .......... .......... .......... 566 TTATGTAGTC AGACTTGTGG CTGAGCTGCT AGAGCTTTTC CTGGGTTATT TTGTTTTAAA 133037 .......... .......... .......... .......... .......... .......... 566 ATAGAGGGGT TTTGTCAAAT TTTTGTTTGT TGGATGGGCT AATACTTTCT TTAAAATCTC 132977 .......... .......... .......... .......... .......... .......... 566 TATATGGTCT ATCAGGGTTC AGGTGTCGAG TTTCAGCACA AACTTTAGAA TACAGTTCAG 132917 .......... .......... .......... .......... .......... .......... 566 GAAGTGTCTG TCATGTTTGT TTGACTTGAC AATTATTCAA TACGATGTCT CCAATTTGTT 132857 .......... .......... .......... .......... .......... .......... 566 GTTCACATAT CATATTTACC GATATTAGTA AGTTGCATGT GGTTTGCAGA TCTTTATAGG 132797 .......... .......... .......... .......... .......... .......... 566 TCATATTGTT TTAATGCAAA TGTGTTTGGT ATGAAAGAAA ATGTTTTCTT GTTTTTAGTT 132737 .......... .......... .......... .......... .......... .......... 566 TGTGATGAAA TATATTTTGC AGAAAATTTC CTTCAAATTC CTTGCTATTT TGTTAAGATG 132677 .......... .......... .......... .......... .......... .......... 566 CATATCACTT ACAGCATTTT ATTTGAATCG ACAGTTTTAA TCCTCTTGAT TCATACATAT 132617 |||||| |||||||||| |||||||||| .......... .......... .......... ....TTTTAA TCCTCTTGAT TCATACATAT 592 GGTTTGAACT CTATG 132602 |||||||||| ||||| GGTTTGAACT CTATG 607 hqPGS_C08HBa0251H04.1-_SGN-E328376+ (134896 134816,134592 134282,133705 133618,133466 133381,132642 132602) ******************************************************************************** EST sequence 102 +strand 531 n (File: SGN-E271433+) 1 CTCAAAAAGA TGTCTGTTGT GAATCAGCAA TTTTTCAACC CCATATGGAA GCCATGTGTT 61 TCTTCATGTC CATTGTTAGC TGCACAGTTT GTTGTGATGC CAATGAGTAA TAGAGAGCTT 121 TCTGTTGTTT CCACCATTGC TGCTGCAAAA TCTACAGCTT CGATTGGTGA AGGAAATGAT 181 GATTATGTTG ATATTAGTAG TGGGAAAGCA GCAGCCAAAG GGTCGGGTAC TACGGCGAGA 241 GGAAGGAGGT TGTTGAAGGT TAGGGAGGAG AAGAGGAAAC GGGAATATGA ACGTCTTCAC 301 AATTACCCTG CTTGGGCAAA AGTGTTGGAA GATGCCTGTA AACATGATAC AGAGCTTCGT 361 GCTGTGCTTG GTGATAGCAT TGGAAATCCA GAGCTTATGA GGAAAAAGGT CGAAGAGAGA 421 GTTCGAACAA AGGGCCGAAA TTTTCAGAAG TCTAAAACAG GATCAGTTCT TGCATTCAAA 481 GTCAGCTTTA GAGATTNTAA TCCTCTTGAT TCATACATAT GGGTTGAACT C Predicted gene structure (within gDNA segment 135444 to 132401): Exon 1 134824 134816 ( 9 n); cDNA 1 9 ( 9 n); score: 1.000 Intron 1 134815 134593 ( 223 n); Pd: 0.655 (s: 0), Pa: 0.777 (s: 0.96) Exon 2 134592 134282 ( 311 n); cDNA 10 320 ( 311 n); score: 0.994 Intron 2 134281 133706 ( 576 n); Pd: 0.996 (s: 1.00), Pa: 0.987 (s: 1.00) Exon 3 133705 133618 ( 88 n); cDNA 321 408 ( 88 n); score: 1.000 Intron 3 133617 133467 ( 151 n); Pd: 0.987 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 4 133466 133381 ( 86 n); cDNA 409 494 ( 86 n); score: 1.000 Intron 4 133380 132643 ( 738 n); Pd: 0.895 (s: 1.00), Pa: 0.147 (s: 0) Exon 5 132642 132606 ( 37 n); cDNA 495 531 ( 37 n); score: 0.946 MATCH C08HBa0251H04.1- SGN-E271433+ 0.996 531 1.000 C PGS_C08HBa0251H04.1-_SGN-E271433+ (134824 134816,134592 134282,133705 133618,133466 133381,132642 132606) Alignment (genomic DNA sequence = upper lines): CTCAAAAAGG TTATCAAGAT TACTTTTTTT TGAGTTCTGT GTTGAATTTT TTAGCTTTGG 134765 ||||||||| CTCAAAAAG. .......... .......... .......... .......... .......... 9 TAGTTCTTTT TTTTTTTTTT TGTATGTAAT TGAGTTCTGT GTTGATTTTC TGGGACTTTC 134705 .......... .......... .......... .......... .......... .......... 9 TTGAATTTGG GTAAATGAAA AATTCATTTT TTTTCTCTGA ATTCTGTGTA AACCCAAATT 134645 .......... .......... .......... .......... .......... .......... 9 TGTAGGGTTT ATGGTATGCT AAAACCCTTA ATGCAATTCC ATTTTTATGC AGATGTCTGT 134585 |||||||| .......... .......... .......... .......... .......... ..ATGTCTGT 17 TGTAAATCAA CAATTTTTCA ACCCCATATG GAAGCCATGT GTTTCTTCAT GTCCATTGTT 134525 ||| ||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGTGAATCAG CAATTTTTCA ACCCCATATG GAAGCCATGT GTTTCTTCAT GTCCATTGTT 77 AGCTGCACAG TTTGTTGTGA TGCCAATGAG TAATAGAGAG CTTTCTGTTG TTTCCACCAT 134465 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCTGCACAG TTTGTTGTGA TGCCAATGAG TAATAGAGAG CTTTCTGTTG TTTCCACCAT 137 TGCTGCTGCA AAATCTACAG CTTCGATTGG TGAAGGAAAT GATGATTATG TTGATATTAG 134405 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGCTGCTGCA AAATCTACAG CTTCGATTGG TGAAGGAAAT GATGATTATG TTGATATTAG 197 TAGTGGGAAA GCAGCAGCCA AAGGGTCGGG TACTACGGCG AGAGGAAGGA GGTTGTTGAA 134345 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAGTGGGAAA GCAGCAGCCA AAGGGTCGGG TACTACGGCG AGAGGAAGGA GGTTGTTGAA 257 GGTTAGGGAG GAGAAGAGGA AACGGGAATA TGAACGTCTT CACAATTACC CTGCTTGGGC 134285 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GGTTAGGGAG GAGAAGAGGA AACGGGAATA TGAACGTCTT CACAATTACC CTGCTTGGGC 317 AAAGTATATC TCTTAACTAC TTTGCCTATT TGATGCTATA TTGTGTGTTT ATATGTATTT 134225 ||| AAA....... .......... .......... .......... .......... .......... 320 GTTCTGTCCC ACACAAATGG TTTACCTGTA ACTAGTTGAT ACCCCCATGA AAAAGTGATC 134165 .......... .......... .......... .......... .......... .......... 320 ATATAGGAAT GCTTTAGAGG TAGAATACAC TTTGAATTTT GATAAATGCG TTGCTTGTGA 134105 .......... .......... .......... .......... .......... .......... 320 AGTATGTGTC CTTGAGTGTC TTATAGATTT ACCCGCTATT TGATGTTTTA TGTATTCGTT 134045 .......... .......... .......... .......... .......... .......... 320 GTGTTAAAAT GGTATTTGAT ACTTTCATGA TTACCATATA TATCTCACAC AAATCGTTTA 133985 .......... .......... .......... .......... .......... .......... 320 CTTGTAACTA ATTAGTATCT CTAAGAAAAA TAATCACATA GGAATGCTTG CGAGTCCGAA 133925 .......... .......... .......... .......... .......... .......... 320 TACTTTGAAT TTGAGAAATG CATTGCTTGT GAAGTATGTG TGCGTGTATG ACATATTGAT 133865 .......... .......... .......... .......... .......... .......... 320 TTACCCTTTG TTAGTTGGAA GTTGGAAGCC AGACAAGCTA GAGTGCTAGA GTCCATGCAA 133805 .......... .......... .......... .......... .......... .......... 320 CGTTGTTGCC ATTAGGATTC CAACATTGAT TTGATCTTCA CTTTTACTTC ATCTGTGTCC 133745 .......... .......... .......... .......... .......... .......... 320 TTGAGATTGA CTTGTTTATG TTAAAAATGC TATTTTTAGA GTGTTGGAAG ATGCCTGTAA 133685 | |||||||||| |||||||||| .......... .......... .......... .........A GTGTTGGAAG ATGCCTGTAA 341 ACATGATACA GAGCTTCGTG CTGTGCTTGG TGATAGCATT GGAAATCCAG AGCTTATGAG 133625 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATGATACA GAGCTTCGTG CTGTGCTTGG TGATAGCATT GGAAATCCAG AGCTTATGAG 401 GAAAAAGGTA GGTGTAATTT TTATTTGGGA TTTTGAGTTA GGTTTGACAG ACAGACATTT 133565 ||||||| GAAAAAG... .......... .......... .......... .......... .......... 408 GCAGATACTC CACATTTTCG TTGATTTCAG TTCGCTTTAG ATTTCGATTT ATGACTTCCA 133505 .......... .......... .......... .......... .......... .......... 408 ATTGAAATCT GCTTGTTTCC TTTTCTCCAT TTTGATAGGT CGAAGAGAGA GTTCGAACAA 133445 || |||||||||| |||||||||| .......... .......... .......... ........GT CGAAGAGAGA GTTCGAACAA 430 AGGGCCGAAA TTTTCAGAAG TCTAAAACAG GATCAGTTCT TGCATTCAAA GTCAGCTTTA 133385 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGGCCGAAA TTTTCAGAAG TCTAAAACAG GATCAGTTCT TGCATTCAAA GTCAGCTTTA 490 GAGAGTAATT AAATTCTGAC CAAACTTGAT GCTTCTTCTG TTTTCTCTCC AAATTTTCTT 133325 |||| GAGA...... .......... .......... .......... .......... .......... 494 TTCCTATCAC TATCTCCTAG ATATATGAAT AGCATCCAGT GGCCAAATTC CTTCAAAACA 133265 .......... .......... .......... .......... .......... .......... 494 TAATCAAGTG AAGTTTTTAA AGTTCCTTGG TAAAATGTGA TTTTTTTTTG TCTATTTGAT 133205 .......... .......... .......... .......... .......... .......... 494 CTGTAGCTGT TTGTTCTGTT TAACTAGAGT AAATAAATGA AGCAGAATTT TGGGAAGAAT 133145 .......... .......... .......... .......... .......... .......... 494 AACCAGTCTA GGTCGTAGTG TTCTTTTTCA TCGTCAGGAG TTCACTTCTT ATGTAGTCAG 133085 .......... .......... .......... .......... .......... .......... 494 ACTTGTGGCT GAGCTGCTAG AGCTTTTCCT GGGTTATTTT GTTTTAAAAT AGAGGGGTTT 133025 .......... .......... .......... .......... .......... .......... 494 TGTCAAATTT TTGTTTGTTG GATGGGCTAA TACTTTCTTT AAAATCTCTA TATGGTCTAT 132965 .......... .......... .......... .......... .......... .......... 494 CAGGGTTCAG GTGTCGAGTT TCAGCACAAA CTTTAGAATA CAGTTCAGGA AGTGTCTGTC 132905 .......... .......... .......... .......... .......... .......... 494 ATGTTTGTTT GACTTGACAA TTATTCAATA CGATGTCTCC AATTTGTTGT TCACATATCA 132845 .......... .......... .......... .......... .......... .......... 494 TATTTACCGA TATTAGTAAG TTGCATGTGG TTTGCAGATC TTTATAGGTC ATATTGTTTT 132785 .......... .......... .......... .......... .......... .......... 494 AATGCAAATG TGTTTGGTAT GAAAGAAAAT GTTTTCTTGT TTTTAGTTTG TGATGAAATA 132725 .......... .......... .......... .......... .......... .......... 494 TATTTTGCAG AAAATTTCCT TCAAATTCCT TGCTATTTTG TTAAGATGCA TATCACTTAC 132665 .......... .......... .......... .......... .......... .......... 494 AGCATTTTAT TTGAATCGAC AGTTTTAATC CTCTTGATTC ATACATATGG TTTGAACTC 132606 || ||||| |||||||||| |||||||||| |||||||| .......... .......... ..TTNTAATC CTCTTGATTC ATACATATGG GTTGAACTC 531 hqPGS_C08HBa0251H04.1-_SGN-E271433+ (134824 134816,134592 134282,133705 133618,133466 133381,132642 132606) ******************************************************************************** EST sequence 93 +strand 716 n (File: SGN-E316968+) 1 AGCTTATGGA TCAGGTAGCT AATTTAAAGG ACACAATTGC CAAGAAGGAT GAAGAAATTG 61 GGCGGCTGCG GGTTCCCAAA AATAGTGGTA ACGGTGAGAG GCGTAGTGTG AGCTCAACCA 121 GGCATAGCTC TGCCTCTCCA AGAAGGCAAT CTTTAGGCGA TCCACGAACA AACCAAATTT 181 CTGGAGAGAG AAGCTCAAAA CCTACTCAGA AAGCTGCTTC TGACGTGGAC AATAGTTCTG 241 AATACAGCGA CAGACAATCT GACACTGGTT CTCAGCAATC AATGGATGAC TTTAGGCATC 301 ACAGAGATTT CTTTAGACAA TCCAGGCTTG CTGTAGTAGA TGCTGATCTA AATCTGGGTG 361 AACACACTAA TTCAAGGGCT ACTGCGAGAG GAAGTCAAAA TCCTAACGAA GATGTGGTGC 421 TTATTGGGTT TGATGATGCA GATTCTGAAG AGAGATTAAG TGACATATCC GATGGTGTAC 481 TCTCAATGGG AACTGAGACT GATGGTTCAA TCAATAGTAT TGTAGAGTAC ACTCTTTTCC 541 CAGAAACTAC AAAGCCACCT CCAGAGACCC CTGAGAAGCC CTCAATTATA CCTGCTAAAC 601 TTCCACGGCC TACACAAAAG ACGGTGCAAA CAGGATCTTC TCGGATGTCA CTGAAGAAGA 661 GCACCCCTAA AGTCCCGAGT TCTAAAAAAC CTCCTTCCGG AAAACACTTC TGCAGT Predicted gene structure (within gDNA segment 142304 to 139455): Exon 1 141889 141876 ( 14 n); cDNA 1 14 ( 14 n); score: 1.000 Intron 1 141875 141601 ( 275 n); Pd: 0.997 (s: 0), Pa: 0.998 (s: 1.00) Exon 2 141600 141038 ( 563 n); cDNA 15 577 ( 563 n); score: 1.000 Intron 2 141037 140528 ( 510 n); Pd: 0.999 (s: 1.00), Pa: 0.999 (s: 1.00) Exon 3 140527 140427 ( 101 n); cDNA 578 678 ( 101 n); score: 1.000 Intron 3 140426 140273 ( 154 n); Pd: 0.999 (s: 1.00), Pa: 0.996 (s: 0) Exon 4 140272 140236 ( 37 n); cDNA 679 716 ( 38 n); score: 0.932 MATCH C08HBa0251H04.1- SGN-E316968+ 1.000 715 0.999 C PGS_C08HBa0251H04.1-_SGN-E316968+ (141889 141876,141600 141038,140527 140427,140272 140236) Alignment (genomic DNA sequence = upper lines): AGCTTATGGA TCAGGTACCT GACAACACGT TCTAGTAAAT ATCAAAACCT CAGTAGATAA 141830 |||||||||| |||| AGCTTATGGA TCAG...... .......... .......... .......... .......... 14 TGTTCTTCTA GTTGCTCCTA CTTTTTCTCA TTGCCTGCAT CTATAACTGT GCTGTCTACT 141770 .......... .......... .......... .......... .......... .......... 14 TATTTTTTAT GAATCCGACA TCACACTTTG TGCATCTATG ATTAAGACCC ATTTTAATTC 141710 .......... .......... .......... .......... .......... .......... 14 TATCTTGTAC GCTTATCTCT TCTCTAGAGT ACACGAGATT TATGATGCTT GCAATGTTCC 141650 .......... .......... .......... .......... .......... .......... 14 TATTTTCTTT GCTATGGTCA TGTACAAAGT ATTGCTGGTA AATTTTCAGG TAGCTAATTT 141590 | |||||||||| .......... .......... .......... .......... .........G TAGCTAATTT 25 AAAGGACACA ATTGCCAAGA AGGATGAAGA AATTGGGCGG CTGCGGGTTC CCAAAAATAG 141530 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAGGACACA ATTGCCAAGA AGGATGAAGA AATTGGGCGG CTGCGGGTTC CCAAAAATAG 85 TGGTAACGGT GAGAGGCGTA GTGTGAGCTC AACCAGGCAT AGCTCTGCCT CTCCAAGAAG 141470 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTAACGGT GAGAGGCGTA GTGTGAGCTC AACCAGGCAT AGCTCTGCCT CTCCAAGAAG 145 GCAATCTTTA GGCGATCCAC GAACAAACCA AATTTCTGGA GAGAGAAGCT CAAAACCTAC 141410 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCAATCTTTA GGCGATCCAC GAACAAACCA AATTTCTGGA GAGAGAAGCT CAAAACCTAC 205 TCAGAAAGCT GCTTCTGACG TGGACAATAG TTCTGAATAC AGCGACAGAC AATCTGACAC 141350 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCAGAAAGCT GCTTCTGACG TGGACAATAG TTCTGAATAC AGCGACAGAC AATCTGACAC 265 TGGTTCTCAG CAATCAATGG ATGACTTTAG GCATCACAGA GATTTCTTTA GACAATCCAG 141290 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTCTCAG CAATCAATGG ATGACTTTAG GCATCACAGA GATTTCTTTA GACAATCCAG 325 GCTTGCTGTA GTAGATGCTG ATCTAAATCT GGGTGAACAC ACTAATTCAA GGGCTACTGC 141230 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCTTGCTGTA GTAGATGCTG ATCTAAATCT GGGTGAACAC ACTAATTCAA GGGCTACTGC 385 GAGAGGAAGT CAAAATCCTA ACGAAGATGT GGTGCTTATT GGGTTTGATG ATGCAGATTC 141170 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGAGGAAGT CAAAATCCTA ACGAAGATGT GGTGCTTATT GGGTTTGATG ATGCAGATTC 445 TGAAGAGAGA TTAAGTGACA TATCCGATGG TGTACTCTCA ATGGGAACTG AGACTGATGG 141110 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAAGAGAGA TTAAGTGACA TATCCGATGG TGTACTCTCA ATGGGAACTG AGACTGATGG 505 TTCAATCAAT AGTATTGTAG AGTACACTCT TTTCCCAGAA ACTACAAAGC CACCTCCAGA 141050 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTCAATCAAT AGTATTGTAG AGTACACTCT TTTCCCAGAA ACTACAAAGC CACCTCCAGA 565 GACCCCTGAG AAGTGAGTAT TCTACAAATA TGACATATCT GATTATCATT TTGTCCAGTC 140990 |||||||||| || GACCCCTGAG AA........ .......... .......... .......... .......... 577 TTGCCTTACA ATTAGATAAC TTTTTTCTTG AATGTTGACT CACTCATGGA ATCTCTAATT 140930 .......... .......... .......... .......... .......... .......... 577 CCCATTTCCC TGTGATGACC TCAGGTTGAT ATATCAGCAT ATCTTGTATC TTTGTGTTAA 140870 .......... .......... .......... .......... .......... .......... 577 ATTTTGCTTT ACCTGCCTTT GTATTAGCAT TTTTCCTTAT TTCTTTTTCC TTTTTTCATC 140810 .......... .......... .......... .......... .......... .......... 577 TCTTGATCCA TATTTTGTCT TCACAGAAAA TCGACATCAG GTTACATTCT GAATAAGCAT 140750 .......... .......... .......... .......... .......... .......... 577 TTCTTATAGT TTAAAGTCAC AATTCCTGTT TCCAGTTTAA ATGTACTTCA TGACTCTTAG 140690 .......... .......... .......... .......... .......... .......... 577 ATCACATCTC TTCTTAGTAC AGTATAATGT CGAAGATAGC TTATAGTTGT TTGGGAAAAG 140630 .......... .......... .......... .......... .......... .......... 577 CAGAATGCCA AGAGTTCCTG TTGAACTCGT TGTTAAAGAC ACCCAGGACA CTCATCAAAT 140570 .......... .......... .......... .......... .......... .......... 577 TTAAAATGAC TATTACTTAT CTCTTTGCTG GTGAATGATC AGGCCCTCAA TTATACCTGC 140510 |||||||| |||||||||| .......... .......... .......... .......... ..GCCCTCAA TTATACCTGC 595 TAAACTTCCA CGGCCTACAC AAAAGACGGT GCAAACAGGA TCTTCTCGGA TGTCACTGAA 140450 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAACTTCCA CGGCCTACAC AAAAGACGGT GCAAACAGGA TCTTCTCGGA TGTCACTGAA 655 GAAGAGCACC CCTAAAGTCC CGAGTATGTG ATTCTCATAT GTTCTTAGTG TTTGCCTTTA 140390 |||||||||| |||||||||| ||| GAAGAGCACC CCTAAAGTCC CGA....... .......... .......... .......... 678 TTTCCCTTCT TTGTTAATAC TCTCAAGAGT CGAGTTAGAG AAGAGAAATG ACAATCAATG 140330 .......... .......... .......... .......... .......... .......... 678 GTTCACTATT TCCTTCTTTC TGAATCATTC CAACTTTTTC TCTTTTCCGC TTTTCAGGTT 140270 ||| .......... .......... .......... .......... .......... .......GTT 681 CTAAAAAACC TCCTTCCGG- AAACACTTCT GCAGT 140236 |||||||||| ||||||||| |||||||||| ||||| CTAAAAAACC TCCTTCCGGA AAACACTTCT GCAGT 716 hqPGS_C08HBa0251H04.1-_SGN-E316968+ (141889 141876,141600 141038,140527 140427,140272 140236) ******************************************************************************** EST sequence 26 -strand 366 n (File: SGN-E548479-) 1 AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 61 ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 121 ACACGATTGT TGTGTCATCT TCCATTGCTG TGGAAGATGT GTAGGAACTT CAGCTTGTAG 181 CAAACTGCAC TGATCAGATT GAATTTCTTT AGGTGTATTT GCTCCCTGCC TCCCCCTACT 241 CGAAGTGAAG GAGTAAAGGA GGGCGGCGTG GGGTTTGACA TTGTGTAGTG TGTACATGTA 301 GCTCTCTTTA TGAACTTGCT GATGCATTTT TAGGCTATGA AATAGTGTTG ACTGAAAAAA 361 AAAAAA Predicted gene structure (within gDNA segment 140875 to 139201): Exon 1 140265 139908 ( 358 n); cDNA 1 358 ( 358 n); score: 0.997 MATCH C08HBa0251H04.1- SGN-E548479- 0.997 358 0.978 C PGS_C08HBa0251H04.1-_SGN-E548479- (140265 139908) Alignment (genomic DNA sequence = upper lines): AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 140206 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 60 ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 140146 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 120 ACACGATTGT TGTGTCATCT TCCATTGCTG TGGAAGATGT GTAGGAACTT CAGCTTGTAG 140086 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACACGATTGT TGTGTCATCT TCCATTGCTG TGGAAGATGT GTAGGAACTT CAGCTTGTAG 180 CAAACTGCAC TGATCAGATT GAATTTCTTT AGGTGTATTT GCTCCCTGCC TCCCCCTACT 140026 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAAACTGCAC TGATCAGATT GAATTTCTTT AGGTGTATTT GCTCCCTGCC TCCCCCTACT 240 CGAAGTGAAG GAGTAAAGGA GGGCGGCGTG GGGTTTGACA TTGTGTAGTG TGTACATGTA 139966 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGAAGTGAAG GAGTAAAGGA GGGCGGCGTG GGGTTTGACA TTGTGTAGTG TGTACATGTA 300 GCTCTCTTTA TGAACTTGCT GATGCATTTT TAGGCTATGA AATAGTGTTG ACTGATAA 139908 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||| || GCTCTCTTTA TGAACTTGCT GATGCATTTT TAGGCTATGA AATAGTGTTG ACTGAAAA 358 hqPGS_C08HBa0251H04.1-_SGN-E548479- (140265 139908) ******************************************************************************** EST sequence 103 +strand 366 n (File: SGN-E541013+) 1 AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 61 ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 121 ACACGATTGT TGTGTCATCT TCCTTGCGTG GGAAAGATGT GTAGGAACTT CAGCTTGTAG 181 CAAACTGCAC TGATCAGATT GAATTTCTTT AGGTGTATTT GCTCCCTGCC TCCCCCTACT 241 CGAAGTGAAG GAGTAAAGGA GGGCGGCGTG GGGTTTGACA TTGTGTAGTG TGTACATGTA 301 GCTCTCTTTA TGAACTTGCT GATGCATTTT TAGGCTATGA AATAGTGTTG ACTGAAAAAA 361 AAAAAA Predicted gene structure (within gDNA segment 140865 to 139191): Exon 1 140265 139908 ( 358 n); cDNA 1 358 ( 358 n); score: 0.982 MATCH C08HBa0251H04.1- SGN-E541013+ 0.982 358 0.978 C PGS_C08HBa0251H04.1-_SGN-E541013+ (140265 139908) Alignment (genomic DNA sequence = upper lines): AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 140206 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAACCTCCT TCCGGAAACA CTTCTGCAGT TAGGTCTTCC AAAAGATGGC AATAGTACAG 60 ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 140146 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACATTAAATT GTCGAAGAGG ACAGACACTT GACGATCCTT CTTTGTAGAT TAGACTGTAG 120 ACACGATTGT TGTGTCATCT TCCATTGC-T GTGGAAGATG TGTAGGAACT TCAGCTTGTA 140087 |||||||||| |||||||||| ||| |||| | | | |||||| |||||||||| |||||||||| ACACGATTGT TGTGTCATCT TCC-TTGCGT GGGAAAGATG TGTAGGAACT TCAGCTTGTA 179 GCAAACTGCA CTGATCAGAT TGAATTTCTT TAGGTGTATT TGCTCCCTGC CTCCCCCTAC 140027 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GCAAACTGCA CTGATCAGAT TGAATTTCTT TAGGTGTATT TGCTCCCTGC CTCCCCCTAC 239 TCGAAGTGAA GGAGTAAAGG AGGGCGGCGT GGGGTTTGAC ATTGTGTAGT GTGTACATGT 139967 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCGAAGTGAA GGAGTAAAGG AGGGCGGCGT GGGGTTTGAC ATTGTGTAGT GTGTACATGT 299 AGCTCTCTTT ATGAACTTGC TGATGCATTT TTAGGCTATG AAATAGTGTT GACTGATAA 139908 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||| || AGCTCTCTTT ATGAACTTGC TGATGCATTT TTAGGCTATG AAATAGTGTT GACTGAAAA 358 hqPGS_C08HBa0251H04.1-_SGN-E541013+ (140265 139908) ******************************************************************************** EST sequence 66 +strand 365 n (File: SGN-E293454+) 1 AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 61 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 121 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 181 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 241 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 301 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAAAAA 361 AAAAA Predicted gene structure (within gDNA segment 140864 to 139191): Exon 1 140264 139908 ( 357 n); cDNA 1 357 ( 357 n); score: 0.997 MATCH C08HBa0251H04.1- SGN-E293454+ 0.997 357 0.978 C PGS_C08HBa0251H04.1-_SGN-E293454+ (140264 139908) Alignment (genomic DNA sequence = upper lines): AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 140205 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 60 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 140145 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 120 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 140085 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 180 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 140025 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 240 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 139965 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 300 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGATAA 139908 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| || CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAA 357 hqPGS_C08HBa0251H04.1-_SGN-E293454+ (140264 139908) ******************************************************************************** EST sequence 67 +strand 365 n (File: SGN-E293455+) 1 AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 61 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 121 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 181 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 241 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 301 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAAAAA 361 AAAAA Predicted gene structure (within gDNA segment 140864 to 139191): Exon 1 140264 139908 ( 357 n); cDNA 1 357 ( 357 n); score: 0.997 MATCH C08HBa0251H04.1- SGN-E293455+ 0.997 357 0.978 C PGS_C08HBa0251H04.1-_SGN-E293455+ (140264 139908) Alignment (genomic DNA sequence = upper lines): AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 140205 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 60 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 140145 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 120 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 140085 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 180 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 140025 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 240 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 139965 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 300 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGATAA 139908 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| || CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAA 357 hqPGS_C08HBa0251H04.1-_SGN-E293455+ (140264 139908) ******************************************************************************** EST sequence 68 +strand 364 n (File: SGN-E293630+) 1 AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 61 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 121 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 181 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 241 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 301 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAAAAA 361 AAAA Predicted gene structure (within gDNA segment 140864 to 139201): Exon 1 140264 139908 ( 357 n); cDNA 1 357 ( 357 n); score: 0.997 MATCH C08HBa0251H04.1- SGN-E293630+ 0.997 357 0.981 C PGS_C08HBa0251H04.1-_SGN-E293630+ (140264 139908) Alignment (genomic DNA sequence = upper lines): AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 140205 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACCTCCTT CCGGAAACAC TTCTGCAGTT AGGTCTTCCA AAAGATGGCA ATAGTACAGA 60 CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 140145 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATTAAATTG TCGAAGAGGA CAGACACTTG ACGATCCTTC TTTGTAGATT AGACTGTAGA 120 CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 140085 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CACGATTGTT GTGTCATCTT CCATTGCTGT GGAAGATGTG TAGGAACTTC AGCTTGTAGC 180 AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 140025 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAACTGCACT GATCAGATTG AATTTCTTTA GGTGTATTTG CTCCCTGCCT CCCCCTACTC 240 GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 139965 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAGTGAAGG AGTAAAGGAG GGCGGCGTGG GGTTTGACAT TGTGTAGTGT GTACATGTAG 300 CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGATAA 139908 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||| || CTCTCTTTAT GAACTTGCTG ATGCATTTTT AGGCTATGAA ATAGTGTTGA CTGAAAA 357 hqPGS_C08HBa0251H04.1-_SGN-E293630+ (140264 139908) ******************************************************************************** EST sequence 72 +strand 805 n (File: SGN-E262026+) 1 AACATATAAA CAAATCATTA TCTGCTCTTG GGGATGTGAT ATTTGCATTG GCGCAAAAAA 61 GTTCTCATGT GCCATATAGA AATAGCAAAC TAACACAAGT TCTTCAAAGC TCCCTTGGAG 121 GCCAGGCAAA GACACTTATG TTTGTACAGC TCAATCCTGA CGTAGAATCC TACTCAGAAA 181 CCATTAGTAC CCTAAAGTTT GCTGAGAGGG TGTCTGGAGT GGAGTTAGGT GCAGCTCGGA 241 ATAATAAAGA AGGCCGGGGT GTTAAAGAGC TTATGGATCA GGTAGCTAAT TTAAAGGACA 301 CAATTGCCAA GAAGGATGAA GAAATTGGGC GGCTGCGGGT TCCCAAAAAT AGTGGTAACG 361 GTGAGAGGCG TAGTGTGAGC TCAACCAGGC ATAGCTCTGC CTCTCCAAGA AGGCAATCTT 421 TAGGCGATCC ACGAACAAAC CAAATTTCTG GAGAGAGAAG CTCAAAACCT ACTCAGAAAG 481 CTGCTTCTGA CGTGGACAAT AGTTCTGAAT ACAGCGACAG ACAATCTGAC ACTGGTTCTC 541 AGCAATCAAT GGATGACTTT AGGCATCACA GAGATTTCTT TAGACAATCC AGGCTTGCTG 601 TAGTAGATGC TGATCTAAAT CTGGGTGAAC ACACTAATTC AAGGGCTACG GCGAGAGGAA 661 GTCAAAATCC TAACGAAGAT GTGGTGCTTA TTGGGTTTGA TGATGCAGAT TCTGAAGAGA 721 GATTAAGTGA CATATCCGAT GGTGGTACTC TCATGGGAAC TGAGACTGAT GGTTTCATCA 781 TAGTATTGTA GAGTACACTC TTTTC Predicted gene structure (within gDNA segment 142878 to 140466): Exon 1 142278 142162 ( 117 n); cDNA 1 117 ( 117 n); score: 1.000 Intron 1 142161 142040 ( 122 n); Pd: 0.869 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 2 142039 141876 ( 164 n); cDNA 118 281 ( 164 n); score: 1.000 Intron 2 141875 141601 ( 275 n); Pd: 0.997 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 3 141600 141076 ( 525 n); cDNA 282 805 ( 524 n); score: 0.986 MATCH C08HBa0251H04.1- SGN-E262026+ 0.991 806 1.001 C PGS_C08HBa0251H04.1-_SGN-E262026+ (142278 142162,142039 141876,141600 141076) Alignment (genomic DNA sequence = upper lines): AACATATAAA CAAATCATTA TCTGCTCTTG GGGATGTGAT ATTTGCATTG GCGCAAAAAA 142219 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACATATAAA CAAATCATTA TCTGCTCTTG GGGATGTGAT ATTTGCATTG GCGCAAAAAA 60 GTTCTCATGT GCCATATAGA AATAGCAAAC TAACACAAGT TCTTCAAAGC TCCCTTGGTA 142159 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||||||| GTTCTCATGT GCCATATAGA AATAGCAAAC TAACACAAGT TCTTCAAAGC TCCCTTG... 117 GTTAAATATA TTTTCTGTGT TGATTCAAAT TTAAATATTG TTTTACTCTG TAAATAAGTT 142099 .......... .......... .......... .......... .......... .......... 117 TTACTTCTCT TTCCTATTAA AGCCTTTATT TATTTTATTT CACTCATCTC ATGTTACAGG 142039 | .......... .......... .......... .......... .......... .........G 118 AGGCCAGGCA AAGACACTTA TGTTTGTACA GCTCAATCCT GACGTAGAAT CCTACTCAGA 141979 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGGCCAGGCA AAGACACTTA TGTTTGTACA GCTCAATCCT GACGTAGAAT CCTACTCAGA 178 AACCATTAGT ACCCTAAAGT TTGCTGAGAG GGTGTCTGGA GTGGAGTTAG GTGCAGCTCG 141919 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACCATTAGT ACCCTAAAGT TTGCTGAGAG GGTGTCTGGA GTGGAGTTAG GTGCAGCTCG 238 GAATAATAAA GAAGGCCGGG GTGTTAAAGA GCTTATGGAT CAGGTACCTG ACAACACGTT 141859 |||||||||| |||||||||| |||||||||| |||||||||| ||| GAATAATAAA GAAGGCCGGG GTGTTAAAGA GCTTATGGAT CAG....... .......... 281 CTAGTAAATA TCAAAACCTC AGTAGATAAT GTTCTTCTAG TTGCTCCTAC TTTTTCTCAT 141799 .......... .......... .......... .......... .......... .......... 281 TGCCTGCATC TATAACTGTG CTGTCTACTT ATTTTTTATG AATCCGACAT CACACTTTGT 141739 .......... .......... .......... .......... .......... .......... 281 GCATCTATGA TTAAGACCCA TTTTAATTCT ATCTTGTACG CTTATCTCTT CTCTAGAGTA 141679 .......... .......... .......... .......... .......... .......... 281 CACGAGATTT ATGATGCTTG CAATGTTCCT ATTTTCTTTG CTATGGTCAT GTACAAAGTA 141619 .......... .......... .......... .......... .......... .......... 281 TTGCTGGTAA ATTTTCAGGT AGCTAATTTA AAGGACACAA TTGCCAAGAA GGATGAAGAA 141559 || |||||||||| |||||||||| |||||||||| |||||||||| .......... ........GT AGCTAATTTA AAGGACACAA TTGCCAAGAA GGATGAAGAA 323 ATTGGGCGGC TGCGGGTTCC CAAAAATAGT GGTAACGGTG AGAGGCGTAG TGTGAGCTCA 141499 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTGGGCGGC TGCGGGTTCC CAAAAATAGT GGTAACGGTG AGAGGCGTAG TGTGAGCTCA 383 ACCAGGCATA GCTCTGCCTC TCCAAGAAGG CAATCTTTAG GCGATCCACG AACAAACCAA 141439 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACCAGGCATA GCTCTGCCTC TCCAAGAAGG CAATCTTTAG GCGATCCACG AACAAACCAA 443 ATTTCTGGAG AGAGAAGCTC AAAACCTACT CAGAAAGCTG CTTCTGACGT GGACAATAGT 141379 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATTTCTGGAG AGAGAAGCTC AAAACCTACT CAGAAAGCTG CTTCTGACGT GGACAATAGT 503 TCTGAATACA GCGACAGACA ATCTGACACT GGTTCTCAGC AATCAATGGA TGACTTTAGG 141319 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCTGAATACA GCGACAGACA ATCTGACACT GGTTCTCAGC AATCAATGGA TGACTTTAGG 563 CATCACAGAG ATTTCTTTAG ACAATCCAGG CTTGCTGTAG TAGATGCTGA TCTAAATCTG 141259 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATCACAGAG ATTTCTTTAG ACAATCCAGG CTTGCTGTAG TAGATGCTGA TCTAAATCTG 623 GGTGAACACA CTAATTCAAG GGCTACTGCG AGAGGAAGTC AAAATCCTAA CGAAGATGTG 141199 |||||||||| |||||||||| |||||| ||| |||||||||| |||||||||| |||||||||| GGTGAACACA CTAATTCAAG GGCTACGGCG AGAGGAAGTC AAAATCCTAA CGAAGATGTG 683 GTGCTTATTG GGTTTGATGA TGCAGATTCT GAAGAGAGAT TAAGTGACAT ATCCGATGGT 141139 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGCTTATTG GGTTTGATGA TGCAGATTCT GAAGAGAGAT TAAGTGACAT ATCCGATGGT 743 -GTACTCTCA ATGGGAACTG AGACTGATGG TTCAATCAAT AGTATTGTAG AGTACACTCT 141080 |||||||| |||||||||| |||||||||| || ||| || |||||||||| |||||||||| GGTACTCTC- ATGGGAACTG AGACTGATGG TTTCATC-AT AGTATTGTAG AGTACACTCT 801 TTTC 141076 |||| TTTC 805 hqPGS_C08HBa0251H04.1-_SGN-E262026+ (142278 142162,142039 141876,141600 141076) ******************************************************************************** EST sequence 122 +strand 754 n (File: SGN-E332626+) 1 TCAACTACCG AGCTCTGAAT GATCTATTCA ACATCTCCCA GAGTAGGAAA AGTTCCATTG 61 CATATGAAGT TGGTGTTCAA ATGGTTGAGA TATACAATGA GCAAGTGCGA GACTTGCTCT 121 GCAGTGATAC TTCTCAGAAA CGACTTGGGA TTTGGAGTAC TACTCAACCC AATGGATTAG 181 CTGTCCCTGA TGCTAGCATG CATCCAGTCA AATCAACTGC AAATGTCTTA GAATTAATGA 241 ATATTGGCCT AATGAATAGA GCTGTTGGCG CTACTGCTTT AAATGAAAGA AGTAGTCGGT 301 CACATAGTAT CCTCACCGTT CACGTGCGCG GTATAGACTT GGAAACAAAT GATATTCTAC 361 GAGGCTGCCT ACATTTGGTA GATTTGGCTG GCAGTGAAAG AGTAGATCGC TCTGAAGCCA 421 CAGGGGATCG TTTACGAGAA GCACAACATA TAAACAAATC ATTATCTGCT CTTGGGGATG 481 TGATATTTGC ATTGGCGCAA AAAAGTTCTC ATGTGCCATA TAGAAATAGC AAACTAACAC 541 AAGTTCTTCA AAGCTCCCTT GGAGGCCAGG CAAAGACACT TATGTTTGTA CAGCTCAATC 601 CTGACGTAGA ATCCTACTCA GAAACCATTA GTACCCTAAA GTTTGCTGAG AGGGTGTCTG 661 GAGTGGAGTT AGGTGCAGCT CGGAATAATA AAGAAAGGCC GGGTGTTAAA GAGCTTATGG 721 ATCAGGTAGC TAATTTAAAG GACACAATTG CCAA Predicted gene structure (within gDNA segment 143603 to 140962): Exon 1 143003 142862 ( 142 n); cDNA 1 142 ( 142 n); score: 1.000 Intron 1 142861 142669 ( 193 n); Pd: 0.990 (s: 1.00), Pa: 0.000 (s: 1.00) Exon 2 142668 142504 ( 165 n); cDNA 143 307 ( 165 n); score: 1.000 Intron 2 142503 142416 ( 88 n); Pd: 0.951 (s: 1.00), Pa: 0.997 (s: 1.00) Exon 3 142415 142162 ( 254 n); cDNA 308 561 ( 254 n); score: 1.000 Intron 3 142161 142040 ( 122 n); Pd: 0.869 (s: 1.00), Pa: 0.998 (s: 1.00) Exon 4 142039 141876 ( 164 n); cDNA 562 725 ( 164 n); score: 0.982 Intron 4 141875 141601 ( 275 n); Pd: 0.997 (s: 0.94), Pa: 0.998 (s: 0) Exon 5 141600 141572 ( 29 n); cDNA 726 754 ( 29 n); score: 1.000 MATCH C08HBa0251H04.1- SGN-E332626+ 0.996 754 1.000 C PGS_C08HBa0251H04.1-_SGN-E332626+ (143003 142862,142668 142504,142415 142162,142039 141876,141600 141572) Alignment (genomic DNA sequence = upper lines): TCAACTACCG AGCTCTGAAT GATCTATTCA ACATCTCCCA GAGTAGGAAA AGTTCCATTG 142944 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCAACTACCG AGCTCTGAAT GATCTATTCA ACATCTCCCA GAGTAGGAAA AGTTCCATTG 60 CATATGAAGT TGGTGTTCAA ATGGTTGAGA TATACAATGA GCAAGTGCGA GACTTGCTCT 142884 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CATATGAAGT TGGTGTTCAA ATGGTTGAGA TATACAATGA GCAAGTGCGA GACTTGCTCT 120 GCAGTGATAC TTCTCAGAAA CGATATCCTT GTTAAAAAAA TCTATTTGTG TTGTTTGATA 142824 |||||||||| |||||||||| || GCAGTGATAC TTCTCAGAAA CG........ .......... .......... .......... 142 CATTGGCTGT TTTCATTTTC TATGTTCATG TTATACATAT TTATTTCCGT GGATCATTAG 142764 .......... .......... .......... .......... .......... .......... 142 TGCTATATTT TTAAGTTTTC ATTTTCAATA GTTAGTGGAA ATGTCAGAAA TATGTTATGC 142704 .......... .......... .......... .......... .......... .......... 142 CATATGTTTC CTGATGTTTT CTTTAACTTG CGCACACTTG GGATTTGGAG TACTACTCAA 142644 ||||| |||||||||| |||||||||| .......... .......... .......... .....ACTTG GGATTTGGAG TACTACTCAA 167 CCCAATGGAT TAGCTGTCCC TGATGCTAGC ATGCATCCAG TCAAATCAAC TGCAAATGTC 142584 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCAATGGAT TAGCTGTCCC TGATGCTAGC ATGCATCCAG TCAAATCAAC TGCAAATGTC 227 TTAGAATTAA TGAATATTGG CCTAATGAAT AGAGCTGTTG GCGCTACTGC TTTAAATGAA 142524 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTAGAATTAA TGAATATTGG CCTAATGAAT AGAGCTGTTG GCGCTACTGC TTTAAATGAA 287 AGAAGTAGTC GGTCACATAG GTCATCTCTT GTACTAACAC TTCTTATCTC AATTCTGAAC 142464 |||||||||| |||||||||| AGAAGTAGTC GGTCACATAG .......... .......... .......... .......... 307 TTTTTTTCTG TTTGTACATC ATGCTTATTT TAACTCATTA TGATGCAGTA TCCTCACCGT 142404 || |||||||||| .......... .......... .......... .......... ........TA TCCTCACCGT 319 TCACGTGCGC GGTATAGACT TGGAAACAAA TGATATTCTA CGAGGCTGCC TACATTTGGT 142344 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCACGTGCGC GGTATAGACT TGGAAACAAA TGATATTCTA CGAGGCTGCC TACATTTGGT 379 AGATTTGGCT GGCAGTGAAA GAGTAGATCG CTCTGAAGCC ACAGGGGATC GTTTACGAGA 142284 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGATTTGGCT GGCAGTGAAA GAGTAGATCG CTCTGAAGCC ACAGGGGATC GTTTACGAGA 439 AGCACAACAT ATAAACAAAT CATTATCTGC TCTTGGGGAT GTGATATTTG CATTGGCGCA 142224 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGCACAACAT ATAAACAAAT CATTATCTGC TCTTGGGGAT GTGATATTTG CATTGGCGCA 499 AAAAAGTTCT CATGTGCCAT ATAGAAATAG CAAACTAACA CAAGTTCTTC AAAGCTCCCT 142164 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAAAGTTCT CATGTGCCAT ATAGAAATAG CAAACTAACA CAAGTTCTTC AAAGCTCCCT 559 TGGTAGTTAA ATATATTTTC TGTGTTGATT CAAATTTAAA TATTGTTTTA CTCTGTAAAT 142104 || TG........ .......... .......... .......... .......... .......... 561 AAGTTTTACT TCTCTTTCCT ATTAAAGCCT TTATTTATTT TATTTCACTC ATCTCATGTT 142044 .......... .......... .......... .......... .......... .......... 561 ACAGGAGGCC AGGCAAAGAC ACTTATGTTT GTACAGCTCA ATCCTGACGT AGAATCCTAC 141984 |||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ....GAGGCC AGGCAAAGAC ACTTATGTTT GTACAGCTCA ATCCTGACGT AGAATCCTAC 617 TCAGAAACCA TTAGTACCCT AAAGTTTGCT GAGAGGGTGT CTGGAGTGGA GTTAGGTGCA 141924 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCAGAAACCA TTAGTACCCT AAAGTTTGCT GAGAGGGTGT CTGGAGTGGA GTTAGGTGCA 677 GCTCGGAATA ATAAAGAAGG CCGGGGTGTT AAAGAGCTTA TGGATCAGGT ACCTGACAAC 141864 |||||||||| |||||||| | | ||||||| |||||||||| |||||||| GCTCGGAATA ATAAAGAAAG GCCGGGTGTT AAAGAGCTTA TGGATCAG.. .......... 725 ACGTTCTAGT AAATATCAAA ACCTCAGTAG ATAATGTTCT TCTAGTTGCT CCTACTTTTT 141804 .......... .......... .......... .......... .......... .......... 725 CTCATTGCCT GCATCTATAA CTGTGCTGTC TACTTATTTT TTATGAATCC GACATCACAC 141744 .......... .......... .......... .......... .......... .......... 725 TTTGTGCATC TATGATTAAG ACCCATTTTA ATTCTATCTT GTACGCTTAT CTCTTCTCTA 141684 .......... .......... .......... .......... .......... .......... 725 GAGTACACGA GATTTATGAT GCTTGCAATG TTCCTATTTT CTTTGCTATG GTCATGTACA 141624 .......... .......... .......... .......... .......... .......... 725 AAGTATTGCT GGTAAATTTT CAGGTAGCTA ATTTAAAGGA CACAATTGCC AA 141572 ||||||| |||||||||| |||||||||| || .......... .......... ...GTAGCTA ATTTAAAGGA CACAATTGCC AA 754 hqPGS_C08HBa0251H04.1-_SGN-E332626+ (143003 142862,142668 142504,142415 142162,142039 141876,141600 141572) ******************************************************************************** EST sequence 3 +strand 532 n (File: SGN-E324968+) 1 AAGTAGAGGA CTGTTATGAA AGAGAAGGAG GAAGAGAAGA ATCAACTGAT AACAGACGAT 61 GACAGAGATG GAGAAGAAGA ACAACAACAA AGAGGGGAAT GTGGTGATCA CGAAAATGCG 121 GAATCTCAAA CATCGTCGTC TTTAAATGGC AAAGAAATAC TGAAAGCAAT CGAAGTTGTG 181 GAGAGAGATT CTATGGCGAT CGCCCATAGC TACACTTCAC TTTTTGCTTC TCTTCGATCA 241 ACTTTATCAG AGGTTACTAG TACTTCTGTT GATCACATGA TCTGCTTTGG TGATGCAGCA 301 GGACGAGTTC AAGAATGTGC ACTTGATGCA GCAACTAAAG GAAACCGTTA CATAAACTCT 361 TGCTTAAGAT TGAACGAGGA AATGAAAGGA ATTGATAATC TTGCAACCCA GCTGAAAATC 421 ATCCGGAGAA ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA 481 GTGTGGATCT GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT TT Predicted gene structure (within gDNA segment 150283 to 155690): Exon 1 151139 151368 ( 230 n); cDNA 23 252 ( 230 n); score: 1.000 Intron 1 151369 152864 (1496 n); Pd: 0.981 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 152865 152931 ( 67 n); cDNA 253 319 ( 67 n); score: 1.000 Intron 2 152932 154234 (1303 n); Pd: 0.967 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 154235 154283 ( 49 n); cDNA 320 368 ( 49 n); score: 1.000 Intron 3 154284 154605 ( 322 n); Pd: 0.969 (s: 1.00), Pa: 0.862 (s: 1.00) Exon 4 154606 154650 ( 45 n); cDNA 369 413 ( 45 n); score: 1.000 Intron 4 154651 154961 ( 311 n); Pd: 0.990 (s: 1.00), Pa: 0.954 (s: 1.00) Exon 5 154962 155080 ( 119 n); cDNA 414 532 ( 119 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E324968+ 1.000 510 0.959 C PGS_C08HBa0251H04.1+_SGN-E324968+ (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) Alignment (genomic DNA sequence = upper lines): AGAAGGAGGA AGAGAAGAAT CAACTGATAA CAGACGATGA CAGAGATGGA GAAGAAGAAC 151198 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAGGAGGA AGAGAAGAAT CAACTGATAA CAGACGATGA CAGAGATGGA GAAGAAGAAC 82 AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA ATCTCAAACA TCGTCGTCTT 151258 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA ATCTCAAACA TCGTCGTCTT 142 TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA GAGAGATTCT ATGGCGATCG 151318 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA GAGAGATTCT ATGGCGATCG 202 CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC TTTATCAGAG GTTTCTTCTT 151378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC TTTATCAGAG .......... 252 TCTGTCCTTA ATTCTTGCAT TTCCTCATAA TCTTAGTACT CGGTGGTGTT GAGGCCACTG 151438 .......... .......... .......... .......... .......... .......... 252 CTGCAAATAG TGGCGTAGCT TTGAGATAGT GTTATCTGAA AGTAATTATC TTTAGTCCTT 151498 .......... .......... .......... .......... .......... .......... 252 CATCATGTTC AGTGTTTGGC ACGTTTCTTT TTAATTTGGT TACTGATAAA AAGAGAGTCT 151558 .......... .......... .......... .......... .......... .......... 252 TTTTTGTTTT TTTAATTTGG TTTGAGATGA TTGTATGTCA GTGGAGGGAT GATTGTGAGA 151618 .......... .......... .......... .......... .......... .......... 252 TTTAGAGAAT CCTCAATTTA TAATGAACTG GAGTGGATGT TGAGTATAGA TATAGCTTAT 151678 .......... .......... .......... .......... .......... .......... 252 CTTAGTTAGT TTGGGAATTA AGCGAAGAAG TAGTAGTTGG ATAGAAAAGC ATGTTTAAGG 151738 .......... .......... .......... .......... .......... .......... 252 TGATCAACGA GCTGCATGGT GATCTAAGCT CTTTGCCTGC TTTTTGATTG TGCATTTTGC 151798 .......... .......... .......... .......... .......... .......... 252 TTGTAAAATT TCTGAACTGG ATCAGTAGTT GGATAGAAAA GTGTTCTTAT GATGATCAAC 151858 .......... .......... .......... .......... .......... .......... 252 GAGGTGCATG GTGATTTAGG CTCTTCGCCT GCTGTTTGAT TGTATATTTT GCTTGTAAAA 151918 .......... .......... .......... .......... .......... .......... 252 ATGGTAAACT GGAATGAGTA GTTGGATAGA ATATGCGTAT GATGATCAGC GAGCTACATG 151978 .......... .......... .......... .......... .......... .......... 252 GTGATCTAGC CTCTTTGCCT GCTGTTTGAT TGTGCATTTT GCTGGTAAAA TTTCTAAACT 152038 .......... .......... .......... .......... .......... .......... 252 GGAATCAGTT TTCATCTGGG TAGATAGCAA GGTTTTTTCA ATTCATAGCA TTCTGAATTC 152098 .......... .......... .......... .......... .......... .......... 252 TCTGATATGA TCTCTTCTAA GCATCACATT TGACACTTTT CACTTGTAGT TTAAACGGTT 152158 .......... .......... .......... .......... .......... .......... 252 TGGTACGTTG TTGCTTATTC TGCGTAGAGT TATACTTTGT TATCTATCTT CTCTTGGGAT 152218 .......... .......... .......... .......... .......... .......... 252 ATTGCACTTG GGTTTCAGGT CATGTGACTG TCATGTTTCT GATGAAGATT TAGGAATGTT 152278 .......... .......... .......... .......... .......... .......... 252 GCTCCAGTGG CACTTGTATG GTACTTTGGA GGAAAGGAAA GACCACTTGA AGGGGTGGAA 152338 .......... .......... .......... .......... .......... .......... 252 ATTGGAATGC AAGTTTACCA ATTAGGACAT GTCGTTGTTT TTGTTTAATT TTTTGTCTGC 152398 .......... .......... .......... .......... .......... .......... 252 CCGGAGAGAT TACTGTCTGT ATAAATGATT AAGTCGCTTT CAGAGAGAAC CACTAGTTCT 152458 .......... .......... .......... .......... .......... .......... 252 CTTCTTTTTG ATAGATTGCT TGTATACGGA GTTCTTCTCC CACAAGATTA ATAAAATATT 152518 .......... .......... .......... .......... .......... .......... 252 ACTTTATAAT AAGAAACGAT TGGATGAGAT GTCAATCTTA TTGGGAAGAA TTAGTTCGAT 152578 .......... .......... .......... .......... .......... .......... 252 TTTACCTATT TATCCTTAGT ATGGCATGTG ACTGTCATAT GACAATCTTT CAGTGACTTT 152638 .......... .......... .......... .......... .......... .......... 252 TGCCGAGAAG TTATCCGATG ACTAAGTGAG ACTTTTGGGT GAATCTAGAT GGCGAAATGC 152698 .......... .......... .......... .......... .......... .......... 252 AATTCTAGCC ATAGATGGCA TAGTGCCATG TAATTATGAA CCACAAAGTG CCAAAAAGCA 152758 .......... .......... .......... .......... .......... .......... 252 TTACAATTTA GTGGACTTCA TGCATTTGAC ACCTGATATT AGAACTTCTT TTTCTTTTCA 152818 .......... .......... .......... .......... .......... .......... 252 TGTGTGTGTT CTGTCCATGT TTAAAGGAGA TAACATGATT TTGCAGGTTA CTAGTACTTC 152878 |||| |||||||||| .......... .......... .......... .......... ......GTTA CTAGTACTTC 266 TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA GTTCAAGAAT GTGGTATATA 152938 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA GTTCAAGAAT GTG....... 319 CTTTTACGGT TTTACCTCTT CATCTTCAGT TGTTGATGTC CTTACATCAA TAGTTTTTCT 152998 .......... .......... .......... .......... .......... .......... 319 TTTATTCTGC TTGTTGTTCG TTCATTATTT ATTCAACAGA CTAAGAATGA CTGTCTTTAA 153058 .......... .......... .......... .......... .......... .......... 319 GTTCACGCTA GATCCCACAA ATGTTGTTTT GAGGTAAGAT AAACACAAGA TCTAACATAG 153118 .......... .......... .......... .......... .......... .......... 319 TATTGCGCAC TTATCTATCA GTTCTCTTGG AAACATGCAC CTCATCAATA TTCTAAAAGA 153178 .......... .......... .......... .......... .......... .......... 319 AAACCTTCTT AGGTTCATTA AGAATTTAAC TGCCATAAGT TGGTACCATG GATGTACCAG 153238 .......... .......... .......... .......... .......... .......... 319 CTTTATTTGT AGGCAAAGTG GAAGAATTGA TTTTTCCGCT TCTCTCTTTG TTGCTTTAGT 153298 .......... .......... .......... .......... .......... .......... 319 AATCCTTGTT CTAGAAGATG AGGACACTTG GTTTTGCCAG TGATCATTAA TTCAAACTTC 153358 .......... .......... .......... .......... .......... .......... 319 ATCCTAGATG TGACTGCAAG AGGTTTATCT CTGTTCTGCT AACCCCAAAT GCATTATGCA 153418 .......... .......... .......... .......... .......... .......... 319 TGAGACGCCT GGATTTCTCT TGACCAATTT TCTGCATAGT TATCTTTTTT TGGATGTCTC 153478 .......... .......... .......... .......... .......... .......... 319 CTTATTTACC ACTAGGACAA ACTAGTTAGG GTGTCATCCT AAAACTTGAA TTATGGAGAA 153538 .......... .......... .......... .......... .......... .......... 319 TTATTCTGGA GAGTAAGAGA TTTAGGATAG GTAGAAGTAA CAGAATGTAA GGAAGTCTAT 153598 .......... .......... .......... .......... .......... .......... 319 TGGGAAATTG GCGAACTTTT CCCAGCTTCT ATTCTTTGAT GCCTGTGGTA GAAAGAAATA 153658 .......... .......... .......... .......... .......... .......... 319 GTGGATGCTT TGAAGGAAAC CCCTCTCCTA TCCATATTCT GGAATACAGA TTGCTCACAA 153718 .......... .......... .......... .......... .......... .......... 319 ATCTGGAGTA ACCACCACCA GTGCAAATAA TTTTGTTAGC TCCCTAGGAG GTTTATAGAA 153778 .......... .......... .......... .......... .......... .......... 319 GGTCCATTGT TTCCGGGAGC TAACGTTTCT GCTCCAGCTT GTAATTTTTA GAATCTACTG 153838 .......... .......... .......... .......... .......... .......... 319 GATCTTGTTA ATGAAATTTT ACTTTACCAG ATTAAAAAGA AAAGACACAA GTATAAGTAA 153898 .......... .......... .......... .......... .......... .......... 319 GATTAGAGTA TATGCACAGG AAGTTAAGCC AAATAAGGAG TGAAGTCGAA ACGAGATTAG 153958 .......... .......... .......... .......... .......... .......... 319 ATTCAGATAA CTAGACTTGA TTTTCTAGGG AAAAAAGTTT TATTTATAAA AATGTGATCA 154018 .......... .......... .......... .......... .......... .......... 319 TAATTAAAAT AAGAAGTTTG ATAAGTGTGT TATGTAATAG GAGGATACTT ACCAAAGTGA 154078 .......... .......... .......... .......... .......... .......... 319 AAGACAAGTT TTATGTGATT AAATTCCAGA TCTTTCCTTT CCTCATTTTA TTTTCTTAAA 154138 .......... .......... .......... .......... .......... .......... 319 TGCATTCACA TTTCATTAAT ACCTTATTAA CATGTTTTAA GTGATGTACT AAAGTTGAGC 154198 .......... .......... .......... .......... .......... .......... 319 TGCACTAGTA TTAATGTTTG GCTGTTTATC TTGCAGCACT TGATGCAGCA ACTAAAGGAA 154258 |||| |||||||||| |||||||||| .......... .......... .......... ......CACT TGATGCAGCA ACTAAAGGAA 343 ACCGTTACAT AAACTCTTGC TTAAGGTAGT ATTTTTTGTT ATTCTTTAGT CAAGTCCCTG 154318 |||||||||| |||||||||| ||||| ACCGTTACAT AAACTCTTGC TTAAG..... .......... .......... .......... 368 TAGTACCTTC CTGAAAAAAA AAATTATCAG TCTCACCCTA AGGGGTAGTT GGGAGGAAGC 154378 .......... .......... .......... .......... .......... .......... 368 ACCAAGTTAA TGTCCTTATC TTTAGATGGT ATAACATCTT AGATGTTTAT GGTATTCATG 154438 .......... .......... .......... .......... .......... .......... 368 TACGTTTGGT TTTGGTAAAG CAATTCGTCA AGCCTCTAAA TAGGGAACAT ATAACTTGCG 154498 .......... .......... .......... .......... .......... .......... 368 TGTTTCGTAT CAGGTCTATT TTTGCATCAA ATAGTATAAT TACATCCTTC CCACTCCAAA 154558 .......... .......... .......... .......... .......... .......... 368 AATCTACTAT TCTGGCAAAT CTTTATAAGC TAACTCTCTC TTCGCAGATT GAACGAGGAA 154618 ||| |||||||||| .......... .......... .......... .......... .......ATT GAACGAGGAA 381 ATGAAAGGAA TTGATAATCT TGCAACCCAG CTGTATCCTT TCCATGTGAT TATGGTTTGA 154678 |||||||||| |||||||||| |||||||||| || ATGAAAGGAA TTGATAATCT TGCAACCCAG CT........ .......... .......... 413 TGTTAGACAT TATTTCTTCT ACAAGTCTCC AATTCACGTC CACCAATTCT TTGTTTAAAG 154738 .......... .......... .......... .......... .......... .......... 413 TTTGACTAAA GGATGCAATG GTGATTCTTG TCCTTGCAGT TGGCAATTAA CCAGTGGACT 154798 .......... .......... .......... .......... .......... .......... 413 GACAGGTTTC ATTTCATTGT TTTCCTTTTT TATATCAATA GTTTTTCGTT TATTGTCTTG 154858 .......... .......... .......... .......... .......... .......... 413 AACAAGAATT CATGGGCACC TTGCTCCCTG CAATGACATA GTATGACTGT TTAGAACCAA 154918 .......... .......... .......... .......... .......... .......... 413 TCAATATATC ATGTGTTCTT TGTGACTCCT TGATACCTTG AAGGAAAATC ATCCGGAGAA 154978 ||||||| |||||||||| .......... .......... .......... .......... ...GAAAATC ATCCGGAGAA 430 ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA GTGTGGATCT 155038 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA GTGTGGATCT 490 GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT TT 155080 |||||||||| |||||||||| |||||||||| |||||||||| || GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT TT 532 hqPGS_C08HBa0251H04.1+_SGN-E324968+ (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) ******************************************************************************** EST sequence 4 +strand 532 n (File: SGN-E324971+) 1 AAGTAGAGGA CTGTTATGAA AGAGAAGGAG GAAGAGAAGA ATCAACTGAT AACAGACGAT 61 GACAGAGATG GAGAAGAAGA ACAACAACAA AGAGGGGAAT GTGGTGATCA CGAAAATGCG 121 GAATCTCAAA CATCGTCGTC TTTAAATGGC AAAGAAATAC TGAAAGCAAT CGAAGTTGTG 181 GAGAGAGATT CTATGGCGAT CGCCCATAGC TACACTTCAC TTTTTGCTTC TCTTCGATCA 241 ACTTTATCAG AGGTTACTAG TACTTCTGTT GATCACATGA TCTGCTTTGG TGATGCAGCA 301 GGACGAGTTC AAGAATGTGC ACTTGATGCA GCAACTAAAG GAAACCGTTA CATAAACTCT 361 TGCTTAAGAT TGAACGAGGA AATGAAAGGA ATTGATAATC TTGCAACCCA GCTGAAAATC 421 ATCCGGAGAA ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA 481 GTGTGGATCT GTCTTGATGG ACGATGTCAA CTANGTGAGC TGCACATATT TT Predicted gene structure (within gDNA segment 150283 to 155690): Exon 1 151139 151368 ( 230 n); cDNA 23 252 ( 230 n); score: 1.000 Intron 1 151369 152864 (1496 n); Pd: 0.981 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 152865 152931 ( 67 n); cDNA 253 319 ( 67 n); score: 1.000 Intron 2 152932 154234 (1303 n); Pd: 0.967 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 154235 154283 ( 49 n); cDNA 320 368 ( 49 n); score: 1.000 Intron 3 154284 154605 ( 322 n); Pd: 0.969 (s: 1.00), Pa: 0.862 (s: 1.00) Exon 4 154606 154650 ( 45 n); cDNA 369 413 ( 45 n); score: 1.000 Intron 4 154651 154961 ( 311 n); Pd: 0.990 (s: 1.00), Pa: 0.954 (s: 1.00) Exon 5 154962 155080 ( 119 n); cDNA 414 532 ( 119 n); score: 0.992 MATCH C08HBa0251H04.1+ SGN-E324971+ 0.998 510 0.959 C PGS_C08HBa0251H04.1+_SGN-E324971+ (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) Alignment (genomic DNA sequence = upper lines): AGAAGGAGGA AGAGAAGAAT CAACTGATAA CAGACGATGA CAGAGATGGA GAAGAAGAAC 151198 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AGAAGGAGGA AGAGAAGAAT CAACTGATAA CAGACGATGA CAGAGATGGA GAAGAAGAAC 82 AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA ATCTCAAACA TCGTCGTCTT 151258 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA ATCTCAAACA TCGTCGTCTT 142 TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA GAGAGATTCT ATGGCGATCG 151318 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA GAGAGATTCT ATGGCGATCG 202 CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC TTTATCAGAG GTTTCTTCTT 151378 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC TTTATCAGAG .......... 252 TCTGTCCTTA ATTCTTGCAT TTCCTCATAA TCTTAGTACT CGGTGGTGTT GAGGCCACTG 151438 .......... .......... .......... .......... .......... .......... 252 CTGCAAATAG TGGCGTAGCT TTGAGATAGT GTTATCTGAA AGTAATTATC TTTAGTCCTT 151498 .......... .......... .......... .......... .......... .......... 252 CATCATGTTC AGTGTTTGGC ACGTTTCTTT TTAATTTGGT TACTGATAAA AAGAGAGTCT 151558 .......... .......... .......... .......... .......... .......... 252 TTTTTGTTTT TTTAATTTGG TTTGAGATGA TTGTATGTCA GTGGAGGGAT GATTGTGAGA 151618 .......... .......... .......... .......... .......... .......... 252 TTTAGAGAAT CCTCAATTTA TAATGAACTG GAGTGGATGT TGAGTATAGA TATAGCTTAT 151678 .......... .......... .......... .......... .......... .......... 252 CTTAGTTAGT TTGGGAATTA AGCGAAGAAG TAGTAGTTGG ATAGAAAAGC ATGTTTAAGG 151738 .......... .......... .......... .......... .......... .......... 252 TGATCAACGA GCTGCATGGT GATCTAAGCT CTTTGCCTGC TTTTTGATTG TGCATTTTGC 151798 .......... .......... .......... .......... .......... .......... 252 TTGTAAAATT TCTGAACTGG ATCAGTAGTT GGATAGAAAA GTGTTCTTAT GATGATCAAC 151858 .......... .......... .......... .......... .......... .......... 252 GAGGTGCATG GTGATTTAGG CTCTTCGCCT GCTGTTTGAT TGTATATTTT GCTTGTAAAA 151918 .......... .......... .......... .......... .......... .......... 252 ATGGTAAACT GGAATGAGTA GTTGGATAGA ATATGCGTAT GATGATCAGC GAGCTACATG 151978 .......... .......... .......... .......... .......... .......... 252 GTGATCTAGC CTCTTTGCCT GCTGTTTGAT TGTGCATTTT GCTGGTAAAA TTTCTAAACT 152038 .......... .......... .......... .......... .......... .......... 252 GGAATCAGTT TTCATCTGGG TAGATAGCAA GGTTTTTTCA ATTCATAGCA TTCTGAATTC 152098 .......... .......... .......... .......... .......... .......... 252 TCTGATATGA TCTCTTCTAA GCATCACATT TGACACTTTT CACTTGTAGT TTAAACGGTT 152158 .......... .......... .......... .......... .......... .......... 252 TGGTACGTTG TTGCTTATTC TGCGTAGAGT TATACTTTGT TATCTATCTT CTCTTGGGAT 152218 .......... .......... .......... .......... .......... .......... 252 ATTGCACTTG GGTTTCAGGT CATGTGACTG TCATGTTTCT GATGAAGATT TAGGAATGTT 152278 .......... .......... .......... .......... .......... .......... 252 GCTCCAGTGG CACTTGTATG GTACTTTGGA GGAAAGGAAA GACCACTTGA AGGGGTGGAA 152338 .......... .......... .......... .......... .......... .......... 252 ATTGGAATGC AAGTTTACCA ATTAGGACAT GTCGTTGTTT TTGTTTAATT TTTTGTCTGC 152398 .......... .......... .......... .......... .......... .......... 252 CCGGAGAGAT TACTGTCTGT ATAAATGATT AAGTCGCTTT CAGAGAGAAC CACTAGTTCT 152458 .......... .......... .......... .......... .......... .......... 252 CTTCTTTTTG ATAGATTGCT TGTATACGGA GTTCTTCTCC CACAAGATTA ATAAAATATT 152518 .......... .......... .......... .......... .......... .......... 252 ACTTTATAAT AAGAAACGAT TGGATGAGAT GTCAATCTTA TTGGGAAGAA TTAGTTCGAT 152578 .......... .......... .......... .......... .......... .......... 252 TTTACCTATT TATCCTTAGT ATGGCATGTG ACTGTCATAT GACAATCTTT CAGTGACTTT 152638 .......... .......... .......... .......... .......... .......... 252 TGCCGAGAAG TTATCCGATG ACTAAGTGAG ACTTTTGGGT GAATCTAGAT GGCGAAATGC 152698 .......... .......... .......... .......... .......... .......... 252 AATTCTAGCC ATAGATGGCA TAGTGCCATG TAATTATGAA CCACAAAGTG CCAAAAAGCA 152758 .......... .......... .......... .......... .......... .......... 252 TTACAATTTA GTGGACTTCA TGCATTTGAC ACCTGATATT AGAACTTCTT TTTCTTTTCA 152818 .......... .......... .......... .......... .......... .......... 252 TGTGTGTGTT CTGTCCATGT TTAAAGGAGA TAACATGATT TTGCAGGTTA CTAGTACTTC 152878 |||| |||||||||| .......... .......... .......... .......... ......GTTA CTAGTACTTC 266 TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA GTTCAAGAAT GTGGTATATA 152938 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ||| TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA GTTCAAGAAT GTG....... 319 CTTTTACGGT TTTACCTCTT CATCTTCAGT TGTTGATGTC CTTACATCAA TAGTTTTTCT 152998 .......... .......... .......... .......... .......... .......... 319 TTTATTCTGC TTGTTGTTCG TTCATTATTT ATTCAACAGA CTAAGAATGA CTGTCTTTAA 153058 .......... .......... .......... .......... .......... .......... 319 GTTCACGCTA GATCCCACAA ATGTTGTTTT GAGGTAAGAT AAACACAAGA TCTAACATAG 153118 .......... .......... .......... .......... .......... .......... 319 TATTGCGCAC TTATCTATCA GTTCTCTTGG AAACATGCAC CTCATCAATA TTCTAAAAGA 153178 .......... .......... .......... .......... .......... .......... 319 AAACCTTCTT AGGTTCATTA AGAATTTAAC TGCCATAAGT TGGTACCATG GATGTACCAG 153238 .......... .......... .......... .......... .......... .......... 319 CTTTATTTGT AGGCAAAGTG GAAGAATTGA TTTTTCCGCT TCTCTCTTTG TTGCTTTAGT 153298 .......... .......... .......... .......... .......... .......... 319 AATCCTTGTT CTAGAAGATG AGGACACTTG GTTTTGCCAG TGATCATTAA TTCAAACTTC 153358 .......... .......... .......... .......... .......... .......... 319 ATCCTAGATG TGACTGCAAG AGGTTTATCT CTGTTCTGCT AACCCCAAAT GCATTATGCA 153418 .......... .......... .......... .......... .......... .......... 319 TGAGACGCCT GGATTTCTCT TGACCAATTT TCTGCATAGT TATCTTTTTT TGGATGTCTC 153478 .......... .......... .......... .......... .......... .......... 319 CTTATTTACC ACTAGGACAA ACTAGTTAGG GTGTCATCCT AAAACTTGAA TTATGGAGAA 153538 .......... .......... .......... .......... .......... .......... 319 TTATTCTGGA GAGTAAGAGA TTTAGGATAG GTAGAAGTAA CAGAATGTAA GGAAGTCTAT 153598 .......... .......... .......... .......... .......... .......... 319 TGGGAAATTG GCGAACTTTT CCCAGCTTCT ATTCTTTGAT GCCTGTGGTA GAAAGAAATA 153658 .......... .......... .......... .......... .......... .......... 319 GTGGATGCTT TGAAGGAAAC CCCTCTCCTA TCCATATTCT GGAATACAGA TTGCTCACAA 153718 .......... .......... .......... .......... .......... .......... 319 ATCTGGAGTA ACCACCACCA GTGCAAATAA TTTTGTTAGC TCCCTAGGAG GTTTATAGAA 153778 .......... .......... .......... .......... .......... .......... 319 GGTCCATTGT TTCCGGGAGC TAACGTTTCT GCTCCAGCTT GTAATTTTTA GAATCTACTG 153838 .......... .......... .......... .......... .......... .......... 319 GATCTTGTTA ATGAAATTTT ACTTTACCAG ATTAAAAAGA AAAGACACAA GTATAAGTAA 153898 .......... .......... .......... .......... .......... .......... 319 GATTAGAGTA TATGCACAGG AAGTTAAGCC AAATAAGGAG TGAAGTCGAA ACGAGATTAG 153958 .......... .......... .......... .......... .......... .......... 319 ATTCAGATAA CTAGACTTGA TTTTCTAGGG AAAAAAGTTT TATTTATAAA AATGTGATCA 154018 .......... .......... .......... .......... .......... .......... 319 TAATTAAAAT AAGAAGTTTG ATAAGTGTGT TATGTAATAG GAGGATACTT ACCAAAGTGA 154078 .......... .......... .......... .......... .......... .......... 319 AAGACAAGTT TTATGTGATT AAATTCCAGA TCTTTCCTTT CCTCATTTTA TTTTCTTAAA 154138 .......... .......... .......... .......... .......... .......... 319 TGCATTCACA TTTCATTAAT ACCTTATTAA CATGTTTTAA GTGATGTACT AAAGTTGAGC 154198 .......... .......... .......... .......... .......... .......... 319 TGCACTAGTA TTAATGTTTG GCTGTTTATC TTGCAGCACT TGATGCAGCA ACTAAAGGAA 154258 |||| |||||||||| |||||||||| .......... .......... .......... ......CACT TGATGCAGCA ACTAAAGGAA 343 ACCGTTACAT AAACTCTTGC TTAAGGTAGT ATTTTTTGTT ATTCTTTAGT CAAGTCCCTG 154318 |||||||||| |||||||||| ||||| ACCGTTACAT AAACTCTTGC TTAAG..... .......... .......... .......... 368 TAGTACCTTC CTGAAAAAAA AAATTATCAG TCTCACCCTA AGGGGTAGTT GGGAGGAAGC 154378 .......... .......... .......... .......... .......... .......... 368 ACCAAGTTAA TGTCCTTATC TTTAGATGGT ATAACATCTT AGATGTTTAT GGTATTCATG 154438 .......... .......... .......... .......... .......... .......... 368 TACGTTTGGT TTTGGTAAAG CAATTCGTCA AGCCTCTAAA TAGGGAACAT ATAACTTGCG 154498 .......... .......... .......... .......... .......... .......... 368 TGTTTCGTAT CAGGTCTATT TTTGCATCAA ATAGTATAAT TACATCCTTC CCACTCCAAA 154558 .......... .......... .......... .......... .......... .......... 368 AATCTACTAT TCTGGCAAAT CTTTATAAGC TAACTCTCTC TTCGCAGATT GAACGAGGAA 154618 ||| |||||||||| .......... .......... .......... .......... .......ATT GAACGAGGAA 381 ATGAAAGGAA TTGATAATCT TGCAACCCAG CTGTATCCTT TCCATGTGAT TATGGTTTGA 154678 |||||||||| |||||||||| |||||||||| || ATGAAAGGAA TTGATAATCT TGCAACCCAG CT........ .......... .......... 413 TGTTAGACAT TATTTCTTCT ACAAGTCTCC AATTCACGTC CACCAATTCT TTGTTTAAAG 154738 .......... .......... .......... .......... .......... .......... 413 TTTGACTAAA GGATGCAATG GTGATTCTTG TCCTTGCAGT TGGCAATTAA CCAGTGGACT 154798 .......... .......... .......... .......... .......... .......... 413 GACAGGTTTC ATTTCATTGT TTTCCTTTTT TATATCAATA GTTTTTCGTT TATTGTCTTG 154858 .......... .......... .......... .......... .......... .......... 413 AACAAGAATT CATGGGCACC TTGCTCCCTG CAATGACATA GTATGACTGT TTAGAACCAA 154918 .......... .......... .......... .......... .......... .......... 413 TCAATATATC ATGTGTTCTT TGTGACTCCT TGATACCTTG AAGGAAAATC ATCCGGAGAA 154978 ||||||| |||||||||| .......... .......... .......... .......... ...GAAAATC ATCCGGAGAA 430 ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA GTGTGGATCT 155038 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG TTTCCCATGA GTGTGGATCT 490 GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT TT 155080 |||||||||| |||||||||| ||| |||||| |||||||||| || GTCTTGATGG ACGATGTCAA CTANGTGAGC TGCACATATT TT 532 hqPGS_C08HBa0251H04.1+_SGN-E324971+ (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) ******************************************************************************** EST sequence 34 +strand 646 n (File: SGN-E556545+) 1 CAGAGATGGA GAAGAAGAAC AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA 61 ATCTCAAACA TCGTCGTCTT TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA 121 GAGAGATTCT ATGGCGATCG CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC 181 TTTATCAGAG GTTACTAGTA CTTCTGTTGA TCACATGATC TGCTTTGGTG ATGCAGCAGG 241 ACGAGTTCAA GAATGTGCAC TTGATGCAGC AACTAAAGGA AACCGTTACA TAAACTCTTG 301 CTTAAGATTG AACGAGGAAA TGAAAGGAAT TGATAATCTT GCAACCCAGC TGAAAATCAT 361 CCGGAGAAAC GTAGATGCAT TGGACTCGGC TGTAAATAGG CTAGTTCGTT TCCCATGAGT 421 GTGGATCTGT CTTGATGGAC GATGTCAACT AGGTGAGCTG CACATATTTT GTGGTGCTTT 481 ATATATGAGT GAGATGCCAG TTAAGTCTTG CTTCTTTGAA GGTTTAAGAA TAAAACAGTG 541 TGTAATTGAA TGAATTGCAA GGCTGATACA TTTTCTAACC CATAGAAAAG CTTTCACCAA 601 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAA Predicted gene structure (within gDNA segment 150579 to 156289): Exon 1 151179 151368 ( 190 n); cDNA 1 190 ( 190 n); score: 1.000 Intron 1 151369 152864 (1496 n); Pd: 0.981 (s: 1.00), Pa: 0.992 (s: 1.00) Exon 2 152865 152931 ( 67 n); cDNA 191 257 ( 67 n); score: 1.000 Intron 2 152932 154234 (1303 n); Pd: 0.967 (s: 1.00), Pa: 0.991 (s: 1.00) Exon 3 154235 154283 ( 49 n); cDNA 258 306 ( 49 n); score: 1.000 Intron 3 154284 154605 ( 322 n); Pd: 0.969 (s: 1.00), Pa: 0.862 (s: 1.00) Exon 4 154606 154650 ( 45 n); cDNA 307 351 ( 45 n); score: 1.000 Intron 4 154651 154961 ( 311 n); Pd: 0.990 (s: 1.00), Pa: 0.954 (s: 1.00) Exon 5 154962 155208 ( 247 n); cDNA 352 598 ( 247 n); score: 1.000 PPA cDNA 599 646 MATCH C08HBa0251H04.1+ SGN-E556545+ 1.000 598 0.926 C PGS_C08HBa0251H04.1+_SGN-E556545+ (151179 151368,152865 152931,154235 154283,154606 154650,154962 155208) Alignment (genomic DNA sequence = upper lines): CAGAGATGGA GAAGAAGAAC AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA 151238 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CAGAGATGGA GAAGAAGAAC AACAACAAAG AGGGGAATGT GGTGATCACG AAAATGCGGA 60 ATCTCAAACA TCGTCGTCTT TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA 151298 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATCTCAAACA TCGTCGTCTT TAAATGGCAA AGAAATACTG AAAGCAATCG AAGTTGTGGA 120 GAGAGATTCT ATGGCGATCG CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC 151358 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAGAGATTCT ATGGCGATCG CCCATAGCTA CACTTCACTT TTTGCTTCTC TTCGATCAAC 180 TTTATCAGAG GTTTCTTCTT TCTGTCCTTA ATTCTTGCAT TTCCTCATAA TCTTAGTACT 151418 |||||||||| TTTATCAGAG .......... .......... .......... .......... .......... 190 CGGTGGTGTT GAGGCCACTG CTGCAAATAG TGGCGTAGCT TTGAGATAGT GTTATCTGAA 151478 .......... .......... .......... .......... .......... .......... 190 AGTAATTATC TTTAGTCCTT CATCATGTTC AGTGTTTGGC ACGTTTCTTT TTAATTTGGT 151538 .......... .......... .......... .......... .......... .......... 190 TACTGATAAA AAGAGAGTCT TTTTTGTTTT TTTAATTTGG TTTGAGATGA TTGTATGTCA 151598 .......... .......... .......... .......... .......... .......... 190 GTGGAGGGAT GATTGTGAGA TTTAGAGAAT CCTCAATTTA TAATGAACTG GAGTGGATGT 151658 .......... .......... .......... .......... .......... .......... 190 TGAGTATAGA TATAGCTTAT CTTAGTTAGT TTGGGAATTA AGCGAAGAAG TAGTAGTTGG 151718 .......... .......... .......... .......... .......... .......... 190 ATAGAAAAGC ATGTTTAAGG TGATCAACGA GCTGCATGGT GATCTAAGCT CTTTGCCTGC 151778 .......... .......... .......... .......... .......... .......... 190 TTTTTGATTG TGCATTTTGC TTGTAAAATT TCTGAACTGG ATCAGTAGTT GGATAGAAAA 151838 .......... .......... .......... .......... .......... .......... 190 GTGTTCTTAT GATGATCAAC GAGGTGCATG GTGATTTAGG CTCTTCGCCT GCTGTTTGAT 151898 .......... .......... .......... .......... .......... .......... 190 TGTATATTTT GCTTGTAAAA ATGGTAAACT GGAATGAGTA GTTGGATAGA ATATGCGTAT 151958 .......... .......... .......... .......... .......... .......... 190 GATGATCAGC GAGCTACATG GTGATCTAGC CTCTTTGCCT GCTGTTTGAT TGTGCATTTT 152018 .......... .......... .......... .......... .......... .......... 190 GCTGGTAAAA TTTCTAAACT GGAATCAGTT TTCATCTGGG TAGATAGCAA GGTTTTTTCA 152078 .......... .......... .......... .......... .......... .......... 190 ATTCATAGCA TTCTGAATTC TCTGATATGA TCTCTTCTAA GCATCACATT TGACACTTTT 152138 .......... .......... .......... .......... .......... .......... 190 CACTTGTAGT TTAAACGGTT TGGTACGTTG TTGCTTATTC TGCGTAGAGT TATACTTTGT 152198 .......... .......... .......... .......... .......... .......... 190 TATCTATCTT CTCTTGGGAT ATTGCACTTG GGTTTCAGGT CATGTGACTG TCATGTTTCT 152258 .......... .......... .......... .......... .......... .......... 190 GATGAAGATT TAGGAATGTT GCTCCAGTGG CACTTGTATG GTACTTTGGA GGAAAGGAAA 152318 .......... .......... .......... .......... .......... .......... 190 GACCACTTGA AGGGGTGGAA ATTGGAATGC AAGTTTACCA ATTAGGACAT GTCGTTGTTT 152378 .......... .......... .......... .......... .......... .......... 190 TTGTTTAATT TTTTGTCTGC CCGGAGAGAT TACTGTCTGT ATAAATGATT AAGTCGCTTT 152438 .......... .......... .......... .......... .......... .......... 190 CAGAGAGAAC CACTAGTTCT CTTCTTTTTG ATAGATTGCT TGTATACGGA GTTCTTCTCC 152498 .......... .......... .......... .......... .......... .......... 190 CACAAGATTA ATAAAATATT ACTTTATAAT AAGAAACGAT TGGATGAGAT GTCAATCTTA 152558 .......... .......... .......... .......... .......... .......... 190 TTGGGAAGAA TTAGTTCGAT TTTACCTATT TATCCTTAGT ATGGCATGTG ACTGTCATAT 152618 .......... .......... .......... .......... .......... .......... 190 GACAATCTTT CAGTGACTTT TGCCGAGAAG TTATCCGATG ACTAAGTGAG ACTTTTGGGT 152678 .......... .......... .......... .......... .......... .......... 190 GAATCTAGAT GGCGAAATGC AATTCTAGCC ATAGATGGCA TAGTGCCATG TAATTATGAA 152738 .......... .......... .......... .......... .......... .......... 190 CCACAAAGTG CCAAAAAGCA TTACAATTTA GTGGACTTCA TGCATTTGAC ACCTGATATT 152798 .......... .......... .......... .......... .......... .......... 190 AGAACTTCTT TTTCTTTTCA TGTGTGTGTT CTGTCCATGT TTAAAGGAGA TAACATGATT 152858 .......... .......... .......... .......... .......... .......... 190 TTGCAGGTTA CTAGTACTTC TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA 152918 |||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ......GTTA CTAGTACTTC TGTTGATCAC ATGATCTGCT TTGGTGATGC AGCAGGACGA 244 GTTCAAGAAT GTGGTATATA CTTTTACGGT TTTACCTCTT CATCTTCAGT TGTTGATGTC 152978 |||||||||| ||| GTTCAAGAAT GTG....... .......... .......... .......... .......... 257 CTTACATCAA TAGTTTTTCT TTTATTCTGC TTGTTGTTCG TTCATTATTT ATTCAACAGA 153038 .......... .......... .......... .......... .......... .......... 257 CTAAGAATGA CTGTCTTTAA GTTCACGCTA GATCCCACAA ATGTTGTTTT GAGGTAAGAT 153098 .......... .......... .......... .......... .......... .......... 257 AAACACAAGA TCTAACATAG TATTGCGCAC TTATCTATCA GTTCTCTTGG AAACATGCAC 153158 .......... .......... .......... .......... .......... .......... 257 CTCATCAATA TTCTAAAAGA AAACCTTCTT AGGTTCATTA AGAATTTAAC TGCCATAAGT 153218 .......... .......... .......... .......... .......... .......... 257 TGGTACCATG GATGTACCAG CTTTATTTGT AGGCAAAGTG GAAGAATTGA TTTTTCCGCT 153278 .......... .......... .......... .......... .......... .......... 257 TCTCTCTTTG TTGCTTTAGT AATCCTTGTT CTAGAAGATG AGGACACTTG GTTTTGCCAG 153338 .......... .......... .......... .......... .......... .......... 257 TGATCATTAA TTCAAACTTC ATCCTAGATG TGACTGCAAG AGGTTTATCT CTGTTCTGCT 153398 .......... .......... .......... .......... .......... .......... 257 AACCCCAAAT GCATTATGCA TGAGACGCCT GGATTTCTCT TGACCAATTT TCTGCATAGT 153458 .......... .......... .......... .......... .......... .......... 257 TATCTTTTTT TGGATGTCTC CTTATTTACC ACTAGGACAA ACTAGTTAGG GTGTCATCCT 153518 .......... .......... .......... .......... .......... .......... 257 AAAACTTGAA TTATGGAGAA TTATTCTGGA GAGTAAGAGA TTTAGGATAG GTAGAAGTAA 153578 .......... .......... .......... .......... .......... .......... 257 CAGAATGTAA GGAAGTCTAT TGGGAAATTG GCGAACTTTT CCCAGCTTCT ATTCTTTGAT 153638 .......... .......... .......... .......... .......... .......... 257 GCCTGTGGTA GAAAGAAATA GTGGATGCTT TGAAGGAAAC CCCTCTCCTA TCCATATTCT 153698 .......... .......... .......... .......... .......... .......... 257 GGAATACAGA TTGCTCACAA ATCTGGAGTA ACCACCACCA GTGCAAATAA TTTTGTTAGC 153758 .......... .......... .......... .......... .......... .......... 257 TCCCTAGGAG GTTTATAGAA GGTCCATTGT TTCCGGGAGC TAACGTTTCT GCTCCAGCTT 153818 .......... .......... .......... .......... .......... .......... 257 GTAATTTTTA GAATCTACTG GATCTTGTTA ATGAAATTTT ACTTTACCAG ATTAAAAAGA 153878 .......... .......... .......... .......... .......... .......... 257 AAAGACACAA GTATAAGTAA GATTAGAGTA TATGCACAGG AAGTTAAGCC AAATAAGGAG 153938 .......... .......... .......... .......... .......... .......... 257 TGAAGTCGAA ACGAGATTAG ATTCAGATAA CTAGACTTGA TTTTCTAGGG AAAAAAGTTT 153998 .......... .......... .......... .......... .......... .......... 257 TATTTATAAA AATGTGATCA TAATTAAAAT AAGAAGTTTG ATAAGTGTGT TATGTAATAG 154058 .......... .......... .......... .......... .......... .......... 257 GAGGATACTT ACCAAAGTGA AAGACAAGTT TTATGTGATT AAATTCCAGA TCTTTCCTTT 154118 .......... .......... .......... .......... .......... .......... 257 CCTCATTTTA TTTTCTTAAA TGCATTCACA TTTCATTAAT ACCTTATTAA CATGTTTTAA 154178 .......... .......... .......... .......... .......... .......... 257 GTGATGTACT AAAGTTGAGC TGCACTAGTA TTAATGTTTG GCTGTTTATC TTGCAGCACT 154238 |||| .......... .......... .......... .......... .......... ......CACT 261 TGATGCAGCA ACTAAAGGAA ACCGTTACAT AAACTCTTGC TTAAGGTAGT ATTTTTTGTT 154298 |||||||||| |||||||||| |||||||||| |||||||||| ||||| TGATGCAGCA ACTAAAGGAA ACCGTTACAT AAACTCTTGC TTAAG..... .......... 306 ATTCTTTAGT CAAGTCCCTG TAGTACCTTC CTGAAAAAAA AAATTATCAG TCTCACCCTA 154358 .......... .......... .......... .......... .......... .......... 306 AGGGGTAGTT GGGAGGAAGC ACCAAGTTAA TGTCCTTATC TTTAGATGGT ATAACATCTT 154418 .......... .......... .......... .......... .......... .......... 306 AGATGTTTAT GGTATTCATG TACGTTTGGT TTTGGTAAAG CAATTCGTCA AGCCTCTAAA 154478 .......... .......... .......... .......... .......... .......... 306 TAGGGAACAT ATAACTTGCG TGTTTCGTAT CAGGTCTATT TTTGCATCAA ATAGTATAAT 154538 .......... .......... .......... .......... .......... .......... 306 TACATCCTTC CCACTCCAAA AATCTACTAT TCTGGCAAAT CTTTATAAGC TAACTCTCTC 154598 .......... .......... .......... .......... .......... .......... 306 TTCGCAGATT GAACGAGGAA ATGAAAGGAA TTGATAATCT TGCAACCCAG CTGTATCCTT 154658 ||| |||||||||| |||||||||| |||||||||| |||||||||| || .......ATT GAACGAGGAA ATGAAAGGAA TTGATAATCT TGCAACCCAG CT........ 351 TCCATGTGAT TATGGTTTGA TGTTAGACAT TATTTCTTCT ACAAGTCTCC AATTCACGTC 154718 .......... .......... .......... .......... .......... .......... 351 CACCAATTCT TTGTTTAAAG TTTGACTAAA GGATGCAATG GTGATTCTTG TCCTTGCAGT 154778 .......... .......... .......... .......... .......... .......... 351 TGGCAATTAA CCAGTGGACT GACAGGTTTC ATTTCATTGT TTTCCTTTTT TATATCAATA 154838 .......... .......... .......... .......... .......... .......... 351 GTTTTTCGTT TATTGTCTTG AACAAGAATT CATGGGCACC TTGCTCCCTG CAATGACATA 154898 .......... .......... .......... .......... .......... .......... 351 GTATGACTGT TTAGAACCAA TCAATATATC ATGTGTTCTT TGTGACTCCT TGATACCTTG 154958 .......... .......... .......... .......... .......... .......... 351 AAGGAAAATC ATCCGGAGAA ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG 155018 ||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ...GAAAATC ATCCGGAGAA ACGTAGATGC ATTGGACTCG GCTGTAAATA GGCTAGTTCG 408 TTTCCCATGA GTGTGGATCT GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT 155078 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTTCCCATGA GTGTGGATCT GTCTTGATGG ACGATGTCAA CTAGGTGAGC TGCACATATT 468 TTGTGGTGCT TTATATATGA GTGAGATGCC AGTTAAGTCT TGCTTCTTTG AAGGTTTAAG 155138 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGTGGTGCT TTATATATGA GTGAGATGCC AGTTAAGTCT TGCTTCTTTG AAGGTTTAAG 528 AATAAAACAG TGTGTAATTG AATGAATTGC AAGGCTGATA CATTTTCTAA CCCATAGAAA 155198 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATAAAACAG TGTGTAATTG AATGAATTGC AAGGCTGATA CATTTTCTAA CCCATAGAAA 588 AGCTTTCACC 155208 |||||||||| AGCTTTCACC 598 hqPGS_C08HBa0251H04.1+_SGN-E556545+ (151179 151368,152865 152931,154235 154283,154606 154650,154962 155208) ******************************************************************************** EST sequence 27 +strand 646 n (File: SGN-E314977+) 1 AACTGATAAC AGACGATGAC AGAGATGGAG AAGAAGAACA ACAACAAAGA GGGGAATGTG 61 GTGATCACGA AAATGCGGAA TCTCAAACAT CGTCGTCTTT AAATGGCAAA GAAATACTGA 121 AAGCAATCGA AGTTGTGGAG AGAGATTCTA TGGCGATCGC CCATAGCTAC ACTTCACTTT 181 TTGCTTCTCT TCGATCAACT TTATCAGAGG TTTCTTCTTT CTGTCCTTAA TTCTTGCATT 241 TCCTCATAAT CTTAGTACTC GGTGGTGTTG AGGCCACTGC TGCAAATAGT GGCGTAGCTT 301 TGAGATAGTG TTATCTGAAA GTAATTATCT TTAGTCCTTC ATCATGTTCA GTGTTTGGCA 361 CGTTTCTTTT TAATTTGGTT ACTGATAAAA AGAGAGTCTT TTTTGTTTTT TTAATTTGGT 421 TTGAGATGAT TGTATGTCAG TGGAGGGATG ATTGTGAGAT TTAAAGAATC CTCAATTTAT 481 AATGAACTGG AGTGGATGTT GAGTATAGAT ATAGCTTATC TTAGTTAGTT TGGGAATTAA 541 GCGAAGAAGT AGTAGTTGGA TAGAAAAGCA TGTTTTAGGT GATCAACGAG CTGGCATGGT 601 GATCTAAGCT CTTTGCCTGC TTTTTGATTG TGCATTTTGC TTGGAA Predicted gene structure (within gDNA segment 150560 to 152441): Exon 1 151160 151804 ( 645 n); cDNA 1 646 ( 646 n); score: 0.991 MATCH C08HBa0251H04.1+ SGN-E314977+ 0.991 645 0.998 C PGS_C08HBa0251H04.1+_SGN-E314977+ (151160 151804) Alignment (genomic DNA sequence = upper lines): AACTGATAAC AGACGATGAC AGAGATGGAG AAGAAGAACA ACAACAAAGA GGGGAATGTG 151219 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AACTGATAAC AGACGATGAC AGAGATGGAG AAGAAGAACA ACAACAAAGA GGGGAATGTG 60 GTGATCACGA AAATGCGGAA TCTCAAACAT CGTCGTCTTT AAATGGCAAA GAAATACTGA 151279 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTGATCACGA AAATGCGGAA TCTCAAACAT CGTCGTCTTT AAATGGCAAA GAAATACTGA 120 AAGCAATCGA AGTTGTGGAG AGAGATTCTA TGGCGATCGC CCATAGCTAC ACTTCACTTT 151339 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAGCAATCGA AGTTGTGGAG AGAGATTCTA TGGCGATCGC CCATAGCTAC ACTTCACTTT 180 TTGCTTCTCT TCGATCAACT TTATCAGAGG TTTCTTCTTT CTGTCCTTAA TTCTTGCATT 151399 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGCTTCTCT TCGATCAACT TTATCAGAGG TTTCTTCTTT CTGTCCTTAA TTCTTGCATT 240 TCCTCATAAT CTTAGTACTC GGTGGTGTTG AGGCCACTGC TGCAAATAGT GGCGTAGCTT 151459 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCCTCATAAT CTTAGTACTC GGTGGTGTTG AGGCCACTGC TGCAAATAGT GGCGTAGCTT 300 TGAGATAGTG TTATCTGAAA GTAATTATCT TTAGTCCTTC ATCATGTTCA GTGTTTGGCA 151519 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGAGATAGTG TTATCTGAAA GTAATTATCT TTAGTCCTTC ATCATGTTCA GTGTTTGGCA 360 CGTTTCTTTT TAATTTGGTT ACTGATAAAA AGAGAGTCTT TTTTGTTTTT TTAATTTGGT 151579 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGTTTCTTTT TAATTTGGTT ACTGATAAAA AGAGAGTCTT TTTTGTTTTT TTAATTTGGT 420 TTGAGATGAT TGTATGTCAG TGGAGGGATG ATTGTGAGAT TTAGAGAATC CTCAATTTAT 151639 |||||||||| |||||||||| |||||||||| |||||||||| ||| |||||| |||||||||| TTGAGATGAT TGTATGTCAG TGGAGGGATG ATTGTGAGAT TTAAAGAATC CTCAATTTAT 480 AATGAACTGG AGTGGATGTT GAGTATAGAT ATAGCTTATC TTAGTTAGTT TGGGAATTAA 151699 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AATGAACTGG AGTGGATGTT GAGTATAGAT ATAGCTTATC TTAGTTAGTT TGGGAATTAA 540 GCGAAGAAGT AGTAGTTGGA TAGAAAAGCA TGTTTAAGGT GATCAACGAG CT-GCATGGT 151758 |||||||||| |||||||||| |||||||||| ||||| |||| |||||||||| || ||||||| GCGAAGAAGT AGTAGTTGGA TAGAAAAGCA TGTTTTAGGT GATCAACGAG CTGGCATGGT 600 GATCTAAGCT CTTTGCCTGC TTTTTGATTG TGCATTTTGC TTGTAA 151804 |||||||||| |||||||||| |||||||||| |||||||||| ||| || GATCTAAGCT CTTTGCCTGC TTTTTGATTG TGCATTTTGC TTGGAA 646 hqPGS_C08HBa0251H04.1+_SGN-E314977+ (151160 151804) ******************************************************************************** EST sequence 62 -strand 336 n (File: SGN-E237330-) 1 CGAGGAAATG AAAGGAATTG ATAATCTTGC AACCCAGCTG AAAATCATCC GGAGAAACGT 61 AGATGCATTG GACTCGGCTG TAAATAGGCT AGTTCGTTTC CCATGAGTGT GGATCTGTCT 121 TGATGGACGA TGTCAACTAG GTGAGCTGCA CATATTTTGT GGTGCTTTAT ATATGAGTGA 181 GATGCCAGTT AAGTCTTGCT TCTTTGAAGG TTTAAGAATA AAACAGTGTG TAATTGAATG 241 AATTGCAAGG CTGATACATT TTCTAACCCA TAGAAAAGCT TTCACCATTG TTGCTTTTGC 301 ATACAATTTT TACCTCACTA AAAAAAAAAA AAAAAA Predicted gene structure (within gDNA segment 154002 to 156002): Exon 1 154612 154650 ( 39 n); cDNA 1 39 ( 39 n); score: 1.000 Intron 1 154651 154961 ( 311 n); Pd: 0.990 (s: 0), Pa: 0.954 (s: 1.00) Exon 2 154962 155241 ( 280 n); cDNA 40 319 ( 280 n); score: 1.000 PPA cDNA 320 336 MATCH C08HBa0251H04.1+ SGN-E237330- 1.000 319 0.949 C PGS_C08HBa0251H04.1+_SGN-E237330- (154612 154650,154962 155241) Alignment (genomic DNA sequence = upper lines): CGAGGAAATG AAAGGAATTG ATAATCTTGC AACCCAGCTG TATCCTTTCC ATGTGATTAT 154671 |||||||||| |||||||||| |||||||||| ||||||||| CGAGGAAATG AAAGGAATTG ATAATCTTGC AACCCAGCT. .......... .......... 39 GGTTTGATGT TAGACATTAT TTCTTCTACA AGTCTCCAAT TCACGTCCAC CAATTCTTTG 154731 .......... .......... .......... .......... .......... .......... 39 TTTAAAGTTT GACTAAAGGA TGCAATGGTG ATTCTTGTCC TTGCAGTTGG CAATTAACCA 154791 .......... .......... .......... .......... .......... .......... 39 GTGGACTGAC AGGTTTCATT TCATTGTTTT CCTTTTTTAT ATCAATAGTT TTTCGTTTAT 154851 .......... .......... .......... .......... .......... .......... 39 TGTCTTGAAC AAGAATTCAT GGGCACCTTG CTCCCTGCAA TGACATAGTA TGACTGTTTA 154911 .......... .......... .......... .......... .......... .......... 39 GAACCAATCA ATATATCATG TGTTCTTTGT GACTCCTTGA TACCTTGAAG GAAAATCATC 154971 |||||||||| .......... .......... .......... .......... .......... GAAAATCATC 49 CGGAGAAACG TAGATGCATT GGACTCGGCT GTAAATAGGC TAGTTCGTTT CCCATGAGTG 155031 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CGGAGAAACG TAGATGCATT GGACTCGGCT GTAAATAGGC TAGTTCGTTT CCCATGAGTG 109 TGGATCTGTC TTGATGGACG ATGTCAACTA GGTGAGCTGC ACATATTTTG TGGTGCTTTA 155091 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGATCTGTC TTGATGGACG ATGTCAACTA GGTGAGCTGC ACATATTTTG TGGTGCTTTA 169 TATATGAGTG AGATGCCAGT TAAGTCTTGC TTCTTTGAAG GTTTAAGAAT AAAACAGTGT 155151 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TATATGAGTG AGATGCCAGT TAAGTCTTGC TTCTTTGAAG GTTTAAGAAT AAAACAGTGT 229 GTAATTGAAT GAATTGCAAG GCTGATACAT TTTCTAACCC ATAGAAAAGC TTTCACCATT 155211 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GTAATTGAAT GAATTGCAAG GCTGATACAT TTTCTAACCC ATAGAAAAGC TTTCACCATT 289 GTTGCTTTTG CATACAATTT TTACCTCACT 155241 |||||||||| |||||||||| |||||||||| GTTGCTTTTG CATACAATTT TTACCTCACT 319 hqPGS_C08HBa0251H04.1+_SGN-E237330- (154612 154650,154962 155241) ******************************************************************************** EST sequence 61 -strand 300 n (File: SGN-E237221-) 1 GAAAATCATC CGGAGAAACG TAGATGCATT GGACTCGGCT GTAAATAGGC TAGTTCGTTT 61 CCCATGAGTG TGGATCTGTC TTGATGGACG ATGTCAACTA GGTGAGCTGC ACATATTTTG 121 TGGTGCTTTA TATATGAGTG AGATGCCAGT TAAGTCTTGC TTCTTTGAAG GTTTAAGAAT 181 AAAACAGTGT GTAATTGAAT GAATTGCAAG GCTGATACAT TTTCTAACCC ATAGAAAAGC 241 TTTCACCATT GTTGCTTTTG CATACAATTT TTACCTCACT AAAAAAAAAA AAAAAAAACT Predicted gene structure (within gDNA segment 154352 to 156032): Exon 1 154962 155241 ( 280 n); cDNA 1 280 ( 280 n); score: 1.000 PPA cDNA 281 299 MATCH C08HBa0251H04.1+ SGN-E237221- 1.000 280 0.933 C PGS_C08HBa0251H04.1+_SGN-E237221- (154962 155241) Alignment (genomic DNA sequence = upper lines): GAAAATCATC CGGAGAAACG TAGATGCATT GGACTCGGCT GTAAATAGGC TAGTTCGTTT 155021 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| GAAAATCATC CGGAGAAACG TAGATGCATT GGACTCGGCT GTAAATAGGC TAGTTCGTTT 60 CCCATGAGTG TGGATCTGTC TTGATGGACG ATGTCAACTA GGTGAGCTGC ACATATTTTG 155081 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CCCATGAGTG TGGATCTGTC TTGATGGACG ATGTCAACTA GGTGAGCTGC ACATATTTTG 120 TGGTGCTTTA TATATGAGTG AGATGCCAGT TAAGTCTTGC TTCTTTGAAG GTTTAAGAAT 155141 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTGCTTTA TATATGAGTG AGATGCCAGT TAAGTCTTGC TTCTTTGAAG GTTTAAGAAT 180 AAAACAGTGT GTAATTGAAT GAATTGCAAG GCTGATACAT TTTCTAACCC ATAGAAAAGC 155201 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| AAAACAGTGT GTAATTGAAT GAATTGCAAG GCTGATACAT TTTCTAACCC ATAGAAAAGC 240 TTTCACCATT GTTGCTTTTG CATACAATTT TTACCTCACT 155241 |||||||||| |||||||||| |||||||||| |||||||||| TTTCACCATT GTTGCTTTTG CATACAATTT TTACCTCACT 280 hqPGS_C08HBa0251H04.1+_SGN-E237221- (154962 155241) ******************************************************************************** EST sequence 49 +strand 652 n (File: SGN-E322356+) 1 CTGTTTTGGC CTTGTTTCTT GAAATCTGAA ACTAAAAAAT TCTCTTTGTA TGGTCCAATA 61 TGGTTGTGGT CCTGAGAACT AAGATCCCAT TTGGGATCAA GATTCAAGAT TTATGCTTTC 121 TTGGTGTTTT ATGATTTTGG TGTCGTTTTT TAGAATTTTG AGCTGATTTT TTCTTCACTG 181 TCATTTTGGT GAGACAAAAG ATTGAAGCTT TGAAAATGGC TATGGGACAC CAAGATCAAG 241 ATGGAGTCCC AGGCAACTTG AGAAAGCAAC TTGCTCTTGC TGTTAGAGGC ATTCAATGGA 301 GCTATGCAAT CTTCTGGTCA ACTGCAGTTA CACAACCAGG GGTGTTGAAA TGGATTGATG 361 GGTACTATAA TGGGGATATC AAGACCAGGA AGACTGTTCA GGCGGGGGAA GTTAATGAAG 421 ACCAGCTGGG GTTGCATAGA ACTGAGCAAT TGAAAGAACT TTATAGTTCC CTCTTAACAA 481 GTGAAAGTGA AGAAGACCTG CAGCCACAGG CCAAAAGGCC GTCGGCCTCA TTATCTCCAG 541 AAGATCTCAC TGATACAGAG TGGTTACCAG GGAAGACCTT AGCACAAATG AAACTGTTTG 601 GCTGTGCAAC GCTCACCAAG CCGAGAGTAA AGTCTTTTCT CGTTCTTTGC TA Predicted gene structure (within gDNA segment 156758 to 158110): Exon 1 157358 157697 ( 340 n); cDNA 1 340 ( 340 n); score: 1.000 MATCH C08HBa0251H04.1+ SGN-E322356+ 1.000 340 0.521 C PGS_C08HBa0251H04.1+_SGN-E322356+ (157358 157697) Alignment (genomic DNA sequence = upper lines): CTGTTTTGGC CTTGTTTCTT GAAATCTGAA ACTAAAAAAT TCTCTTTGTA TGGTCCAATA 157417 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| CTGTTTTGGC CTTGTTTCTT GAAATCTGAA ACTAAAAAAT TCTCTTTGTA TGGTCCAATA 60 TGGTTGTGGT CCTGAGAACT AAGATCCCAT TTGGGATCAA GATTCAAGAT TTATGCTTTC 157477 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TGGTTGTGGT CCTGAGAACT AAGATCCCAT TTGGGATCAA GATTCAAGAT TTATGCTTTC 120 TTGGTGTTTT ATGATTTTGG TGTCGTTTTT TAGAATTTTG AGCTGATTTT TTCTTCACTG 157537 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TTGGTGTTTT ATGATTTTGG TGTCGTTTTT TAGAATTTTG AGCTGATTTT TTCTTCACTG 180 TCATTTTGGT GAGACAAAAG ATTGAAGCTT TGAAAATGGC TATGGGACAC CAAGATCAAG 157597 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| TCATTTTGGT GAGACAAAAG ATTGAAGCTT TGAAAATGGC TATGGGACAC CAAGATCAAG 240 ATGGAGTCCC AGGCAACTTG AGAAAGCAAC TTGCTCTTGC TGTTAGAGGC ATTCAATGGA 157657 |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| |||||||||| ATGGAGTCCC AGGCAACTTG AGAAAGCAAC TTGCTCTTGC TGTTAGAGGC ATTCAATGGA 300 GCTATGCAAT CTTCTGGTCA ACTGCAGTTA CACAACCAGG 157697 |||||||||| |||||||||| |||||||||| |||||||||| GCTATGCAAT CTTCTGGTCA ACTGCAGTTA CACAACCAGG 340 hqPGS_C08HBa0251H04.1+_SGN-E322356+ (157358 157697) Total number of EST alignments reported: 138 ________________________________________________________________________________ Predicted gene locations (20) in segment 1 to 158110: PGL 1 (- strand): 7203 3232 AGS-1 (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3232) SCR (e 1.000 d 0.998 a 0.991,e 1.000 d 0.999 a 0.960,e 1.000 d 0.991 a 0.986,e 1.000 d 0.996 a 0.999,e 1.000 d 0.850 a 0.096,e 1.000 d 0.996 a 1.000,e 1.000) Exon 1 7203 7073 ( 131 n); score: 1.000 Intron 1 7072 6229 ( 844 n); Pd: 0.998 Pa: 0.991 Exon 2 6228 6155 ( 74 n); score: 1.000 Intron 2 6154 6063 ( 92 n); Pd: 0.999 Pa: 0.960 Exon 3 6062 6002 ( 61 n); score: 1.000 Intron 3 6001 5652 ( 350 n); Pd: 0.991 Pa: 0.986 Exon 4 5651 5598 ( 54 n); score: 1.000 Intron 4 5597 4739 ( 859 n); Pd: 0.996 Pa: 0.999 Exon 5 4738 4659 ( 80 n); score: 1.000 Intron 5 4658 4115 ( 544 n); Pd: 0.850 Pa: 0.096 Exon 6 4114 3909 ( 206 n); score: 1.000 Intron 6 3908 3784 ( 125 n); Pd: 0.996 Pa: 1.000 Exon 7 3783 3232 ( 552 n); score: 1.000 PGS (3562 3232) SGN-E237034- PGS (3557 3232) SGN-E237429- PGS (4686 4659,4114 3909,3783 3304) SGN-E373790- PGS (6212 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3583) SGN-E210728+ PGS (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) SGN-E263709+ PGS (7137 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3588) SGN-E261719+ PGS (7139 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3589) SGN-E260745+ PGS (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3690) SGN-E373791+ PGS (7191 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909) SGN-E292336+ PGS (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4003) SGN-E312517+ PGS (7188 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4046) SGN-E288767+ PGS (7203 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 4072) SGN-E312633+ 3-phase translation of AGS-1 (-strand): . . . . . . 7203 GCTCGCGTAGCCGGCGGTGAGTTCGTCGGAGTTAATTTCCAGTGAGAATAGAATTGATTG A R V A G G E F V G V N F Q - E - N - L L A - P A V S S S E L I S S E N R I D - S R S R R - V R R S - F P V R I E L I . . . . . . 7143 AAAGAGATGACGTTGAAATTATACGTAGATCGTATGTCCCAACCTTCTCGTGCAGTTATC K E M T L K L Y V D R M S Q P S R A V I K R - R - N Y T - I V C P N L L V Q L S E R D D V E I I R R S Y V P T F S C S Y . . : . . . . 7083 ATCTTCTGCAA : GTTAAATGGAATAGACTTTGAGGAGATACACATAAATCTCTCCAAGCGC I F C K : L N G I D F E E I H I N L S K R S S A : S - M E - T L R R Y T - I S P S A H L L Q : V K W N R L - G D T H K S L Q A . . . : . . . 6179 CAGCAGTTATCTCCTGAATTTAAAG : AAATTAATCCCATGAAGCAAGTACCAGCTATAATT Q Q L S P E F K : E I N P M K Q V P A I I S S Y L L N L K : K L I P - S K Y Q L - L P A V I S - I - R : N - S H E A S T S Y N . . . : . . . 6027 GATGGAAGATTTAAGCTTTTCGAAAG : TCATGCAATTCTTAGATATCTGGCTTGTGCATTT D G R F K L F E S : H A I L R Y L A C A F M E D L S F S K : V M Q F L D I W L V H F - W K I - A F R K : S C N S - I S G L C I . . : . . . . 5617 CCAGGAATAGCAGATCATTG : GTACCCTGCTGACTTATACAAAAGAGCAAAGGTAGACTCT P G I A D H W : Y P A D L Y K R A K V D S Q E - Q I I : G T L L T Y T K E Q R - T L S R N S R S L : V P C - L I Q K S K G R L . . . . : . . 4698 GTGTTGGACTGGCATCACTCAAACTTGCGTCGCGGTGCAG : CTGGATATATCTTTAATACT V L D W H H S N L R R G A : A G Y I F N T C W T G I T Q T C V A V Q : L D I S L I L C V G L A S L K L A S R C S : W I Y L - Y . . . . . . 4094 GTTCTTGCTCCTGCTTTTGGGCTGCCATTGAATCCACAAGCAGCAGCAGAAGCTGAGAAA V L A P A F G L P L N P Q A A A E A E K F L L L L L G C H - I H K Q Q Q K L R K C S C S C F W A A I E S T S S S R S - E . . . . . . 4034 GTCCTTTTGGCATCTCTTGCAAAAGTCGAGTCTGTTTGGCTCCAGAGAAAAGGACGGTTT V L L A S L A K V E S V W L Q R K G R F S F W H L L Q K S S L F G S R E K D G F S P F G I S C K S R V C L A P E K R T V . . . . . . 3974 TTGCTTGGGAGTGGCCAACCTTCAATTGCAGATCTTAGCTTAGTGTGTGAGATTATGGAA L L G S G Q P S I A D L S L V C E I M E C L G V A N L Q L Q I L A - C V R L W N F A W E W P T F N C R S - L S V - D Y G . : . . . . . 3914 CTTGAG : ATTTTGGATGAGAAGGATCGTGAGCGGATAATAGGCCCATACAAGAGAGTTTTG L E : I L D E K D R E R I I G P Y K R V L L R : F W M R R I V S G - - A H T R E F - T - : D F G - E G S - A D N R P I Q E S F . . . . . . 3729 AAGTGGATTGATGACACAAAGAATGCCATGGAACCTCATTTCCAAGAGGTACATGTGATC K W I D D T K N A M E P H F Q E V H V I S G L M T Q R M P W N L I S K R Y M - S E V D - - H K E C H G T S F P R G T C D . . . . . . 3669 CTCTTCAAAGCTAAAGAGAAGTTCCACAAGCAAAGACATGCTGTAGGAAGTAGCATTCCT L F K A K E K F H K Q R H A V G S S I P S S K L K R S S T S K D M L - E V A F L P L Q S - R E V P Q A K T C C R K - H S . . . . . . 3609 CAATCGAGCAGAAAACCTGACTTGCACTCTAAGATGTGAGAAACCAACAAGGATCCAGGT Q S S R K P D L H S K M - E T N K D P G N R A E N L T C T L R C E K P T R I Q V S I E Q K T - L A L - D V R N Q Q G S R . . . . . . 3549 GCTGCATTGTGCTCGTAGTATCACCGTGCACCATGGCAGATAAACAGTGTGTATGCAAAA A A L C S - Y H R A P W Q I N S V Y A K L H C A R S I T V H H G R - T V C M Q K C C I V L V V S P C T M A D K Q C V C K . . . . . . 3489 GTTGATACAGGAAGGAGAGTTCTGATCCAAGCTTTCCTTACTCATTTTCTCCATGTATAT V D T G R R V L I Q A F L T H F L H V Y L I Q E G E F - S K L S L L I F S M Y M S - Y R K E S S D P S F P Y S F S P C I . . . . . . 3429 GTACTTTATAAGTTTCTATCAGTTGTTAATGTAGTCCACAACATATGTAATCGAACAAAC V L Y K F L S V V N V V H N I C N R T N Y F I S F Y Q L L M - S T T Y V I E Q T C T L - V S I S C - C S P Q H M - S N K . . . . . . 3369 TGAGAGTCCCTTGCTCTTGTGCTTGTAACTTCAGAGCTTATGAACACATTTTTGTCTACA - E S L A L V L V T S E L M N T F L S T E S P L L L C L - L Q S L - T H F C L Q L R V P C S C A C N F R A Y E H I F V Y . . . . . . 3309 ATGTAATCATTAAAGAGTCTTGGTTATACGGAGATTATTCTCTTGATGTTAATGGAGGAA M - S L K S L G Y T E I I L L M L M E E C N H - R V L V I R R L F S - C - W R K N V I I K E S W L Y G D Y S L D V N G G . . 3249 AATTTGTTGTTTTTCTGG N L L F F W I C C F S K F V V F L Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-1_AGS-1_PPS_1 (7146 7073,6228 6155,6062 6002,5651 5598,4738 4659,4114 3909,3783 3571) (frame '1'; 759 bp, 253 residues) 1 LKEMTLKLYV DRMSQPSRAV IIFCKLNGID FEEIHINLSK RQQLSPEFKE INPMKQVPAI 61 IDGRFKLFES HAILRYLACA FPGIADHWYP ADLYKRAKVD SVLDWHHSNL RRGAAGYIFN 121 TVLAPAFGLP LNPQAAAEAE KVLLASLAKV ESVWLQRKGR FLLGSGQPSI ADLSLVCEIM 181 ELEILDEKDR ERIIGPYKRV LKWIDDTKNA MEPHFQEVHV ILFKAKEKFH KQRHAVGSSI 241 PQSSRKPDLH SKM- PGL 2 (- strand): 11507 8036 AGS-1 (11507 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8036) SCR (e 1.000 d 0.595 a 0.992,e 1.000 d 0.985 a 0.974,e 1.000 d 0.987 a 0.997,e 1.000 d 0.995 a 0.987,e 1.000 d 0.883 a 0.000,e 0.850 d 0.997 a 0.999,e 1.000) Exon 1 11507 11376 ( 132 n); score: 1.000 Intron 1 11375 10927 ( 449 n); Pd: 0.595 Pa: 0.992 Exon 2 10926 10853 ( 74 n); score: 1.000 Intron 2 10852 10741 ( 112 n); Pd: 0.985 Pa: 0.974 Exon 3 10740 10680 ( 61 n); score: 1.000 Intron 3 10679 9986 ( 694 n); Pd: 0.987 Pa: 0.997 Exon 4 9985 9932 ( 54 n); score: 1.000 Intron 4 9931 9751 ( 181 n); Pd: 0.995 Pa: 0.987 Exon 5 9750 9671 ( 80 n); score: 1.000 Intron 5 9670 9147 ( 524 n); Pd: 0.883 Pa: 0.000 Exon 6 9146 8941 ( 206 n); score: 0.850 Intron 6 8940 8563 ( 378 n); Pd: 0.997 Pa: 0.999 Exon 7 8562 8036 ( 527 n); score: 1.000 PGS (8419 8036) SGN-E223897- PGS (8267 8036) SGN-E224294- PGS (10910 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8363) SGN-E210728+ PGS (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) SGN-E263709+ PGS (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) SGN-E260745+ PGS (11440 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8370) SGN-E261719+ PGS (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8469) SGN-E373791+ PGS (11442 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941) SGN-E292336+ PGS (11446 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8991) SGN-E311539+ PGS (11507 11376,10926 10853,10740 10680,9985 9932,9750 9671) SGN-E289168+ PGS (11478 11376,10926 10853,10740 10680,9985 9932,9750 9729) SGN-E245867+ 3-phase translation of AGS-1 (-strand): . . . . . . 11507 TCAGTTGACTGTGTCTAAATCCAAAATAAGAAAATCGAAAGTTGAAGCACAGTGGGAGAT S V D C V - I Q N K K I E S - S T V G D Q L T V S K S K I R K S K V E A Q W E I S - L C L N P K - E N R K L K H S G R . . . . . . 11447 TATAGAGATGACGCTCAAATTATACGTAGATCGTATGTCTCAAGCTTGTCGTGAAGTTAT Y R D D A Q I I R R S Y V S S L S - S Y I E M T L K L Y V D R M S Q A C R E V I L - R - R S N Y T - I V C L K L V V K L . . : . . . . 11387 AATCTTCTGCAA : GTTAAATGGGATAGACTTTGAGGAAGTTCATATAGATCTCTCCAAACG N L L Q : V K W D R L - G S S Y R S L Q T I F C K : L N G I D F E E V H I D L S K R - S S A : S - M G - T L R K F I - I S P N . . . : . . . 10878 CCAGCAATTGTCTCCTGAATATCGAG : AAATTAATCCCATCCGGCAAATACCAGCTATAAT P A I V S - I S R : N - S H P A N T S Y N Q Q L S P E Y R : E I N P I R Q I P A I M A S N C L L N I E : K L I P S G K Y Q L - . . . : . . . 10706 GGATGGAAGATTTAAGCTTTCCGAAAG : TCATGCAATTCTTAAATACCTGGCTTGTGCATT G W K I - A F R K : S C N S - I P G L C I D G R F K L S E S : H A I L K Y L A C A F W M E D L S F P K : V M Q F L N T W L V H . . . : . . . 9952 TCCACGAATTGCAGATCATTG : GTATCCAGCTGACTTATACAAAAGAGCAAAGGTTGAATC S T N C R S L : V S S - L I Q K S K G - I P R I A D H W : Y P A D L Y K R A K V E S F H E L Q I I : G I Q L T Y T K E Q R L N . . . . . : . 9711 TGTGTTGGATTGGCATCGTACTAACTTCCCTCGCGGTCCAG : GTTCATATACCTTTTATTC C V G L A S Y - L P S R S R : F I Y L L F V L D W H R T N F P R G P : G S Y T F Y S L C W I G I V L T S L A V Q : V H I P F I . . . . . . 9127 TGTTCTTGCTCCTACTGTTGGGTTGCCTTTAAATACAAAAGCAGCAGCAAGAACTGAGAA C S C S Y C W V A F K Y K S S S K N - E V L A P T V G L P L N T K A A A R T E K L F L L L L L G C L - I Q K Q Q Q E L R . . . . . . 9067 GATGTTTATAGCATGTCTTGCAACGATCGAGTCTGTTTGGCTCCAGAAAAAAGGACGTTT D V Y S M S C N D R V C L A P E K R T F M F I A C L A T I E S V W L Q K K G R F R C L - H V L Q R S S L F G S R K K D V . . . . . . 9007 TTTGCTTGGGAGTGACCAACCTTCAATTGCAGATCTTAGCTTAGCGTGTGAGATTATGCA F A W E - P T F N C R S - L S V - D Y A L L G S D Q P S I A D L S L A C E I M Q F C L G V T N L Q L Q I L A - R V R L C . : . . . . . 8947 ACTTGAG : ATTCTGGATGAGAAGGATCGTGAGCGGATATTAGGCCCATTCAAGAGAGTTCT T - : D S G - E G S - A D I R P I Q E S S L E : I L D E K D R E R I L G P F K R V L N L R : F W M R R I V S G Y - A H S R E F . . . . . . 8509 GAAGTGGCTTGATGATACGAAGAATGCCATGGCACCTCATTTCGAGGAAGTACAATCAAC E V A - - Y E E C H G T S F R G S T I N K W L D D T K N A M A P H F E E V Q S T - S G L M I R R M P W H L I S R K Y N Q . . . . . . 8449 CCTCGCTGGTTATAAGGAGAAGGTGCAGAAGCAAAGAAATACTTTAGGAAGTAAAATTAC P R W L - G E G A E A K K Y F R K - N Y L A G Y K E K V Q K Q R N T L G S K I T P S L V I R R R C R S K E I L - E V K L . . . . . . 8389 TCAATCGGGCAGAAAACCTGTCCTGCAGTCGAATATGTGACACAGGAGTTAGGAAACCAG S I G Q K T C P A V E Y V T Q E L G N Q Q S G R K P V L Q S N M - H R S - E T R L N R A E N L S C S R I C D T G V R K P . . . . . . 8329 ACGCTGCATTATGCTTGAAGCATCGCGGATGATTGTTTCTTCTACACTGTCTATTTGAAG T L H Y A - S I A D D C F F Y T V Y L K R C I M L E A S R M I V S S T L S I - S D A A L C L K H R G - L F L L H C L F E . . . . . . 8269 CTGATCAGGAAGAGATTTCTAGTCCGAGCTTTCCTACTACTTTTTTCAATTCTAAAAACG L I R K R F L V R A F L L L F S I L K T - S G R D F - S E L S Y Y F F Q F - K R A D Q E E I S S P S F P T T F F N S K N . . . . . . 8209 TTTTTCTTGAAATGAGAGTCTGTCATAAACAACCTCTCTACCTCAAACAAGACGGGGTAA F F L K - E S V I N N L S T S N K T G - F S - N E S L S - T T S L P Q T R R G K V F L E M R V C H K Q P L Y L K Q D G V . . . . . . 8149 GATCCGCGTTCATTCTACTACCTTCTCCAGACTTCACACTGGGTCTGTTGCTGTATTTGT D P R S F Y Y L L Q T S H W V C C C I C I R V H S T T F S R L H T G S V A V F V R S A F I L L P S P D F T L G L L L Y L . . . . . . 8089 ATTCATAAAATAAGTGCACCGAATATGTAACAAGACAAGCTGAGTGTTCCTTAA I H K I S A P N M - Q D K L S V P - F I K - V H R I C N K T S - V F L Y S - N K C T E Y V T R Q A E C S L Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-2_AGS-1_PPS_1 (11506 11376,10926 10853,10740 10680,9985 9932,9750 9671,9146 8941,8562 8350) (frame '2'; 816 bp, 272 residues) 1 QLTVSKSKIR KSKVEAQWEI IEMTLKLYVD RMSQACREVI IFCKLNGIDF EEVHIDLSKR 61 QQLSPEYREI NPIRQIPAIM DGRFKLSESH AILKYLACAF PRIADHWYPA DLYKRAKVES 121 VLDWHRTNFP RGPGSYTFYS VLAPTVGLPL NTKAAARTEK MFIACLATIE SVWLQKKGRF 181 LLGSDQPSIA DLSLACEIMQ LEILDEKDRE RILGPFKRVL KWLDDTKNAM APHFEEVQST 241 LAGYKEKVQK QRNTLGSKIT QSGRKPVLQS NM- >C08HBa0251H04.1-_PGL-2_AGS-1_PPS_2 (8297 8082) (frame '0'; 213 bp, 71 residues) 1 LFLLHCLFEA DQEEISSPSF PTTFFNSKNV FLEMRVCHKQ PLYLKQDGVR SAFILLPSPD 61 FTLGLLLYLY S- PGL 3 (+ strand): 12630 16705 AGS-1 (12630 12700,12848 12922,15597 15943,16223 16705) SCR (e 1.000 d 0.000 a 0.990,e 1.000 d 0.942 a 0.983,e 1.000 d 0.999 a 0.965,e 0.981) Exon 1 12630 12700 ( 71 n); score: 1.000 Intron 1 12701 12847 ( 147 n); Pd: 0.000 Pa: 0.990 Exon 2 12848 12922 ( 75 n); score: 1.000 Intron 2 12923 15596 (2674 n); Pd: 0.942 Pa: 0.983 Exon 3 15597 15943 ( 347 n); score: 1.000 Intron 3 15944 16222 ( 279 n); Pd: 0.999 Pa: 0.965 Exon 4 16223 16705 ( 483 n); score: 0.981 PGS (12630 12700,12848 12922,15597 15943,16223 16375) SGN-E302605+ PGS (12638 12700,12848 12922,15597 15905) SGN-E283812+ PGS (12848 12922,15597 15943,16223 16586) SGN-E349881+ PGS (15825 15943,16223 16705) SGN-E302606- 3-phase translation of AGS-1 (+strand): . . . . . . 12630 TACAATTGATCGGCGCCGGACTTTGATCACCGTCGGCGTTTCTAAGCCGTTGCCGGACTA Y N - S A P D F D H R R R F - A V A G L T I D R R R T L I T V G V S K P L P D Y Q L I G A G L - S P S A F L S R C R T . . : . . . . 12690 TGCCGGAGCAA : ATCTCAATCAAGTGAGCTAACCACTCTGTGTTTCAACTTCTGGTTATAA C R S K : S Q S S E L T T L C F N F W L - A G A : N L N Q V S - P L C V S T S G Y K M P E Q : I S I K - A N H S V F Q L L V I . . . : . . . 12897 ATTTTTTAAAGCCTTTGCTCCGTTAG : GTTAGGTAGATACGGATCCTATGACTTCAGAGAT I F - S L C S V R : L G R Y G S Y D F R D F F K A F A P L : G - V D T D P M T S E M N F L K P L L R - : V R - I R I L - L Q R . . . . . . 15631 GGAACCAGTTCAGATTAATGGTGCACCTAGGAGACCTCGTATTCTGCTGGCAGCAAGTGG G T S S D - W C T - E T S Y S A G S K W E P V Q I N G A P R R P R I L L A A S G W N Q F R L M V H L G D L V F C W Q Q V . . . . . . 15691 AAGTGTGGCTTCAATTAAGTTTGCTAATCTATGTCGTTGTTTTTCTGAATGGGCAGAAGT K C G F N - V C - S M S L F F - M G R S S V A S I K F A N L C R C F S E W A E V E V W L Q L S L L I Y V V V F L N G Q K . . . . . . 15751 TAAAGCAGTTGCAACGAAACCTTCTCTTCATTTCATAGACAAAGCTTCGCTTCCGGAAGA - S S C N E T F S S F H R Q S F A S G R K A V A T K P S L H F I D K A S L P E D L K Q L Q R N L L F I S - T K L R F R K . . . . . . 15811 TGTCATTCTTTATACTGATGAGGAGGAATGGTCCACTTGGAAGAAGATTGGTGATAGTGT C H S L Y - - G G M V H L E E D W - - C V I L Y T D E E E W S T W K K I G D S V M S F F I L M R R N G P L G R R L V I V . . . . . . 15871 GCTACACATTGAGCTCCGCAGATGGGCTGATATTATGGTTATTGCCCCTTTGTCAGCAAA A T H - A P Q M G - Y Y G Y C P F V S K L H I E L R R W A D I M V I A P L S A N C Y T L S S A D G L I L W L L P L C Q Q . . : . . . . 15931 CACACTTGGGAAG : ATTGCAGGTGGACTATGTGATAACTTGTTAACCTGCATCGTACGAGC H T W E : D C R W T M - - L V N L H R T S T L G K : I A G G L C D N L L T C I V R A T H L G R : L Q V D Y V I T C - P A S Y E . . . . . . 16270 ATGGGACTACAATAAACCCCTTTTTGTGGCACCAGCCATGAATACATTGATGTGGAATAA M G L Q - T P F C G T S H E Y I D V E - W D Y N K P L F V A P A M N T L M W N N H G T T I N P F L W H Q P - I H - C G I . . . . . . 16330 TCCATTCACAGAACGACACCTTATGGTAATTGATGAGCTTGGAATCTCTCTCATACCACC S I H R T T P Y G N - - A W N L S H T T P F T E R H L M V I D E L G I S L I P P I H S Q N D T L W - L M S L E S L S Y H . . . . . . 16390 AGTTTCAAAAAGACTAGCATGTGGAGATTATGGAAATGGCGCTATGGCTGAACCTTCTCT S F K K T S M W R L W K W R Y G - T F S V S K R L A C G D Y G N G A M A E P S L Q F Q K D - H V E I M E M A L W L N L L . . . . . . 16450 CATCTACTCAACTGTAAGACTCTTCTATGAGTCACGGTCACAATCAGGTGGCATCAACTT H L L N C K T L L - V T V T I R W H Q L I Y S T V R L F Y E S R S Q S G G I N L S S T Q L - D S S M S H G H N Q V A S T . . . . . . 16510 GGCTTGATTCGCAGATCATTAAATTTTATTCATCGGTTTGTACAAGTGGTAGAAATTGTT G L I R R S L N F I H R F V Q V V E I V A - F A D H - I L F I G L Y K W - K L L W L D S Q I I K F Y S S V C T S G R N C . . . . . . 16570 GAAATTCAGGACCTGGAACAGTGTTACTTAGCATACACCAGTTGTGTTTATTTTTCTCCT E I Q D L E Q C Y L A Y T S C V Y F S P K F R T W N S V T - H T P V V F I F L L - N S G P G T V L L S I H Q L C L F F S . . . . . . 16630 AAATTCTTCACCTCTTTAACTTGTGTCATGTTTGTATCCAAGCGGTAACTTTCAATCAAG K F F T S L T C V M F V S K R - L S I K N S S P L - L V S C L Y P S G N F Q S S - I L H L F N L C H V C I Q A V T F N Q . . 16690 TTTCATGTTCATTGTA F H V H C F M F I V V S C S L Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-3_AGS-1_PPS_1 (15602 15943,16223 16516) (frame '2'; 633 bp, 211 residues) 1 VDTDPMTSEM EPVQINGAPR RPRILLAASG SVASIKFANL CRCFSEWAEV KAVATKPSLH 61 FIDKASLPED VILYTDEEEW STWKKIGDSV LHIELRRWAD IMVIAPLSAN TLGKIAGGLC 121 DNLLTCIVRA WDYNKPLFVA PAMNTLMWNN PFTERHLMVI DELGISLIPP VSKRLACGDY 181 GNGAMAEPSL IYSTVRLFYE SRSQSGGINL A- AGS-2 (12853 12922,16223 16705) SCR (e 1.000 d 0.942 a 0.965,e 0.990) Exon 1 12853 12922 ( 70 n); score: 1.000 Intron 1 12923 16222 (3300 n); Pd: 0.942 Pa: 0.965 Exon 2 16223 16705 ( 483 n); score: 0.990 PGS (12853 12922,16223 16705) SGN-E273340- 3-phase translation of AGS-2 (+strand): . . . . . . 12853 AATCAAGTGAGCTAACCACTCTGTGTTTCAACTTCTGGTTATAAATTTTTTAAAGCCTTT N Q V S - P L C V S T S G Y K F F K A F I K - A N H S V F Q L L V I N F L K P L S S E L T T L C F N F W L - I F - S L . : . . . . . 12913 GCTCCGTTAG : ATTGCAGGTGGACTATGTGATAACTTGTTAACCTGCATCGTACGAGCATG A P L : D C R W T M - - L V N L H R T S M L R - : I A G G L C D N L L T C I V R A W C S V R : L Q V D Y V I T C - P A S Y E H . . . . . . 16273 GGACTACAATAAACCCCTTTTTGTGGCACCAGCCATGAATACATTGATGTGGAATAATCC G L Q - T P F C G T S H E Y I D V E - S D Y N K P L F V A P A M N T L M W N N P G T T I N P F L W H Q P - I H - C G I I . . . . . . 16333 ATTCACAGAACGACACCTTATGGTAATTGATGAGCTTGGAATCTCTCTCATACCACCAGT I H R T T P Y G N - - A W N L S H T T S F T E R H L M V I D E L G I S L I P P V H S Q N D T L W - L M S L E S L S Y H Q . . . . . . 16393 TTCAAAAAGACTAGCATGTGGAGATTATGGAAATGGCGCTATGGCTGAACCTTCTCTCAT F K K T S M W R L W K W R Y G - T F S H S K R L A C G D Y G N G A M A E P S L I F Q K D - H V E I M E M A L W L N L L S . . . . . . 16453 CTACTCAACTGTAAGACTCTTCTATGAGTCACGGTCACAATCAGGTGGCATCAACTTGGC L L N C K T L L - V T V T I R W H Q L G Y S T V R L F Y E S R S Q S G G I N L A S T Q L - D S S M S H G H N Q V A S T W . . . . . . 16513 TTGATTCGCAGATCATTAAATTTTATTCATCGGTTTGTACAAGTGGTAGAAATTGTTGAA L I R R S L N F I H R F V Q V V E I V E - F A D H - I L F I G L Y K W - K L L K L D S Q I I K F Y S S V C T S G R N C - . . . . . . 16573 ATTCAGGACCTGGAACAGTGTTACTTAGCATACACCAGTTGTGTTTATTTTTCTCCTAAA I Q D L E Q C Y L A Y T S C V Y F S P K F R T W N S V T - H T P V V F I F L L N N S G P G T V L L S I H Q L C L F F S - . . . . . . 16633 TTCTTCACCTCTTTAACTTGTGTCATGTTTGTATCCAAGCGGTAACTTTCAATCAAGTTT F F T S L T C V M F V S K R - L S I K F S S P L - L V S C L Y P S G N F Q S S F I L H L F N L C H V C I Q A V T F N Q V . . 16693 CATGTTCATTGTA H V H C M F I V S C S L Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-3_AGS-2_PPS_1 (16223 16516) (frame '2'; 291 bp, 97 residues) 1 IAGGLCDNLL TCIVRAWDYN KPLFVAPAMN TLMWNNPFTE RHLMVIDELG ISLIPPVSKR 61 LACGDYGNGA MAEPSLIYST VRLFYESRSQ SGGINLA- AGS-3 (13111 13347) SCR (e 0.958) Exon 1 13111 13347 ( 237 n); score: 0.958 PGS (13111 13347) SGN-E201524+ PGS (13125 13347) SGN-E390994+ PGS (13126 13347) SGN-E287624+ PGS (13129 13347) SGN-E335811+ PGS (13134 13347) SGN-E291487+ PGS (13134 13347) SGN-E232753+ PGS (13134 13347) SGN-E275539+ PGS (13134 13347) SGN-E308395+ PGS (13136 13347) SGN-E295010+ PGS (13139 13347) SGN-E371609+ PGS (13139 13347) SGN-E280323+ PGS (13139 13347) SGN-E290421+ PGS (13140 13347) SGN-E290457+ PGS (13140 13347) SGN-E283296+ PGS (13141 13347) SGN-E390038+ PGS (13141 13347) SGN-E549946+ PGS (13142 13347) SGN-E276053+ PGS (13172 13347) SGN-E239726+ 3-phase translation of AGS-3 (+strand): . . . . . . 13111 TTCGCATCCCAAAGCAAAATCTCAGTATTTTAAGGCAATTTTCTTCAATTCTAAGACAAA F A S Q S K I S V F - G N F L Q F - D K S H P K A K S Q Y F K A I F F N S K T N R I P K Q N L S I L R Q F S S I L R Q . . . . . . 13171 CCCAGTTCAATTCTGTGTTTAATCTTCCTGTTTCATCTGTCTCAAAGCCGATCCGAACAG P S S I L C L I F L F H L S Q S R S E Q P V Q F C V - S S C F I C L K A D P N S T Q F N S V F N L P V S S V S K P I R T . . . . . . 13231 TTTTACCCAATTCTTTGGTTACCTCTTAAGGGTTTTGAGTTGTGGGGTTTAAGATCAGAT F Y P I L W L P L K G F E L W G L R S D F T Q F F G Y L L R V L S C G V - D Q I V L P N S L V T S - G F - V V G F K I R . . . . . . 13291 CATACCATGGCTAGTCAATCCAGCAATCCTTAATCTGTTTATGATTTCACTGTCAAG H T M A S Q S S N P - S V Y D F T V K I P W L V N P A I L N L F M I S L S S Y H G - S I Q Q S L I C L - F H C Q Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-3 (-strand): . . . . . . 13347 CTTGACAGTGAAATCATAAACAGATTAAGGATTGCTGGATTGACTAGCCATGGTATGATC L D S E I I N R L R I A G L T S H G M I L T V K S - T D - G L L D - L A M V - S - Q - N H K Q I K D C W I D - P W Y D . . . . . . 13287 TGATCTTAAACCCCACAACTCAAAACCCTTAAGAGGTAACCAAAGAATTGGGTAAAACTG - S - T P Q L K T L K R - P K N W V K L D L K P H N S K P L R G N Q R I G - N C L I L N P T T Q N P - E V T K E L G K T . . . . . . 13227 TTCGGATCGGCTTTGAGACAGATGAAACAGGAAGATTAAACACAGAATTGAACTGGGTTT F G S A L R Q M K Q E D - T Q N - T G F S D R L - D R - N R K I K H R I E L G L V R I G F E T D E T G R L N T E L N W V . . . . . . 13167 GTCTTAGAATTGAAGAAAATTGCCTTAAAATACTGAGATTTTGCTTTGGGATGCGAA V L E L K K I A L K Y - D F A L G C E S - N - R K L P - N T E I L L W D A C L R I E E N C L K I L R F C F G M R Maximal non-overlapping open reading frames (>= 64 codons): none PGL 4 (+ strand): 21295 24244 AGS-1 (21295 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24244) SCR (e 1.000 d 0.973 a 0.999,e 1.000 d 0.958 a 1.000,e 1.000 d 0.000 a 1.000,e 1.000 d 0.952 a 0.998,e 1.000 d 1.000 a 0.984,e 1.000) Exon 1 21295 21531 ( 237 n); score: 1.000 Intron 1 21532 22499 ( 968 n); Pd: 0.973 Pa: 0.999 Exon 2 22500 22576 ( 77 n); score: 1.000 Intron 2 22577 23257 ( 681 n); Pd: 0.958 Pa: 1.000 Exon 3 23258 23319 ( 62 n); score: 1.000 Intron 3 23320 23468 ( 149 n); Pd: 0.000 Pa: 1.000 Exon 4 23469 23587 ( 119 n); score: 1.000 Intron 4 23588 23701 ( 114 n); Pd: 0.952 Pa: 0.998 Exon 5 23702 23869 ( 168 n); score: 1.000 Intron 5 23870 23969 ( 100 n); Pd: 1.000 Pa: 0.984 Exon 6 23970 24244 ( 275 n); score: 1.000 PGS (21295 21531,22500 22576,23258 23282) SGN-E201524+ PGS (21310 21531,22500 22576,23258 23319,23469 23587,23702 23796) SGN-E390994+ PGS (21311 21531,22500 22576,23258 23319,23469 23535) SGN-E287624+ PGS (21314 21531,22500 22576,23258 23289) SGN-E335811+ PGS (21319 21531,22500 22576,23258 23319,23469 23587,23702 23839) SGN-E275539+ PGS (21319 21531,22500 22576,23258 23319,23469 23587,23702 23812) SGN-E308395+ PGS (21319 21531,22500 22576,23258 23319,23469 23527) SGN-E291487+ PGS (21319 21531,22500 22576,23258 23319,23469 23511) SGN-E232753+ PGS (21321 21531,22500 22576,23258 23319,23469 23587,23702 23740) SGN-E295010+ PGS (21324 21531,22500 22575) SGN-E290421+ PGS (21325 21531,22500 22576,23258 23319,23469 23587,23702 23794) SGN-E290457+ PGS (21325 21531,22500 22576,23258 23319,23469 23587,23702 23737) SGN-E283296+ PGS (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24054) SGN-E390038+ PGS (21326 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24047) SGN-E549946+ PGS (21327 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 23983) SGN-E276053+ PGS (21357 21531,22500 22576,23258 23319,23469 23587,23702 23776) SGN-E239726+ PGS (21464 21531,22500 22576,23258 23319,23469 23549) SGN-E290519+ PGS (21467 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24123) SGN-E550257- PGS (21487 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24244) SGN-E396338- PGS (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24154) SGN-E274781+ PGS (21496 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24057) SGN-E276673+ PGS (21499 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24091) SGN-E276156+ PGS (22552 22576,23258 23319,23469 23587,23702 23869,23970 24168) SGN-E290365- PGS (22553 22576,23258 23319,23469 23587,23702 23869,23970 24198) SGN-E284082+ PGS (23264 23319,23469 23587,23702 23869,23970 24093) SGN-E389614- PGS (23469 23587,23702 23869,23970 24171) SGN-E287358- PGS (23490 23587,23702 23869,23970 24192) SGN-E349657- PGS (23532 23587,23702 23869,23970 24171) SGN-E294534+ 3-phase translation of AGS-1 (+strand): . . . . . . 21295 CTTCTCATCCCAAAGCAAAATCTCAGTATTTTAAGGCGATTTTCTTCGATTCTAAGACAA L L I P K Q N L S I L R R F S S I L R Q F S S Q S K I S V F - G D F L R F - D K S H P K A K S Q Y F K A I F F D S K T . . . . . . 21355 ACCCAGTTCAATTCTGTGTTTAATCTTCCTGTTTCGTCTTTCTCTAAGCCGATCCGAACA T Q F N S V F N L P V S S F S K P I R T P S S I L C L I F L F R L S L S R S E Q N P V Q F C V - S S C F V F L - A D P N . . . . . . 21415 GTTTTACCCAATTCTTTGGTTACCTCTAAGGGTTTTGAGTTGTGGGGTTTAAGATCAGAT V L P N S L V T S K G F E L W G L R S D F Y P I L W L P L R V L S C G V - D Q I S F T Q F F G Y L - G F - V V G F K I R . . . . . . : 21475 CATACCATGGCTAGTCAATCAAGCAACCCTCAATCTGTTTATGATTTCACTGTCAAG : GAT H T M A S Q S S N P Q S V Y D F T V K : D I P W L V N Q A T L N L F M I S L S R : M S Y H G - S I K Q P S I C L - F H C Q : G . . . . . . 22503 GCTAAGGGCAAGGACGTTGATCTCAGTATTTACAAGGGAAAAGTCCTTATCATTGTCAAT A K G K D V D L S I Y K G K V L I I V N L R A R T L I S V F T R E K S L S L S M C - G Q G R - S Q Y L Q G K S P Y H C Q . . : . . . . 22563 GTTGCATCACAGTG : TGGCCTGACTAATTCGAACTACACTGACATGACGGAGTTATACAAG V A S Q C : G L T N S N Y T D M T E L Y K L H H S : V A - L I R T T L T - R S Y T R C C I T V : W P D - F E L H - H D G V I Q . . : . . . . 23304 AAGTACAAGGATCAAG : GTTTGGAGATTCTGGCCTTCCCTTGCAACCAGTTTGGTGGACAG K Y K D Q : G L E I L A F P C N Q F G G Q S T R I K : V W R F W P S L A T S L V D R E V Q G S R : F G D S G L P L Q P V W W T . . . . . . 23513 GAGCCTGGAAACATTGAAGATATCCAGCAAATGGTCTGCACTCGCTTCAAGGCTGAGTAC E P G N I E D I Q Q M V C T R F K A E Y S L E T L K I S S K W S A L A S R L S T G A W K H - R Y P A N G L H S L Q G - V . . : . . . . 23573 CCCATATTTGACAAG : GTTGACGTGAATGGTGATAATGCTGCTCCATTATATAGGTTTCTG P I F D K : V D V N G D N A A P L Y R F L P Y L T R : L T - M V I M L L H Y I G F - P H I - Q : G - R E W - - C C S I I - V S . . . . . . 23747 AAATCAAGCAAAGGTGGGTTCTTTGGAGATGGTATCAAGTGGAACTTCTCCAAATTCCTC K S S K G G F F G D G I K W N F S K F L N Q A K V G S L E M V S S G T S P N S S E I K Q R W V L W R W Y Q V E L L Q I P . . . . . . 23807 ATTGACAAAGAAGGACATGTCGTTGATCGCTACTCTCCAACCACTTCTCCAGCTAGCATG I D K E G H V V D R Y S P T T S P A S M L T K K D M S L I A T L Q P L L Q L A W H - Q R R T C R - S L L S N H F S S - H . : . . . . . 23867 GAG : AAGGATATCAAGAAACTCTTGGGTGTTGCTTAAGCTCTAGAGTCTGGACTCGGCGGT E : K D I K K L L G V A - A L E S G L G G R : R I S R N S W V L L K L - S L D S A V G : E G Y Q E T L G C C L S S R V W T R R . . . . . . 24027 GCTGTCTCTGCATTTTGTGCTAGAAATAATGAACATATCATTGCGAATAAGCTATGTTTG A V S A F C A R N N E H I I A N K L C L L S L H F V L E I M N I S L R I S Y V - C C L C I L C - K - - T Y H C E - A M F . . . . . . 24087 ATGTAATTTTCTTCTTGTTTTAGTTCCTACAGATGTTTGCTACTTTGTACAATTTCAATA M - F S S C F S S Y R C L L L C T I S I C N F L L V L V P T D V C Y F V Q F Q Y D V I F F L F - F L Q M F A T L Y N F N . . . . . . 24147 TACTGATCAATATTTCTATTTACAAGTATTATCAAGTTTTTTCCAACTTTTGGTAAAAAG Y - S I F L F T S I I K F F P T F G K K T D Q Y F Y L Q V L S S F F Q L L V K R I L I N I S I Y K Y Y Q V F S N F W - K . . . . 24207 ATTGCTAGTAAAGATAAATAACACTTCATCATTTCAAA I A S K D K - H F I I S L L V K I N N T S S F Q D C - - R - I T L H H F K Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-4_AGS-1_PPS_1 (21295 21531,22500 22576,23258 23319,23469 23587,23702 23869,23970 24002) (frame '1'; 693 bp, 231 residues) 1 LLIPKQNLSI LRRFSSILRQ TQFNSVFNLP VSSFSKPIRT VLPNSLVTSK GFELWGLRSD 61 HTMASQSSNP QSVYDFTVKD AKGKDVDLSI YKGKVLIIVN VASQCGLTNS NYTDMTELYK 121 KYKDQGLEIL AFPCNQFGGQ EPGNIEDIQQ MVCTRFKAEY PIFDKVDVNG DNAAPLYRFL 181 KSSKGGFFGD GIKWNFSKFL IDKEGHVVDR YSPTTSPASM EKDIKKLLGV A- AGS-2 (21324 21531,22500 22928) SCR (e 0.988 d 0.973 a 0.999,e 1.000) Exon 1 21324 21531 ( 208 n); score: 0.988 Intron 1 21532 22499 ( 968 n); Pd: 0.973 Pa: 0.999 Exon 2 22500 22928 ( 429 n); score: 1.000 PGS (21324 21531,22500 22928) SGN-E371609+ PGS (21324 21531,22500 22819) SGN-E280323+ 3-phase translation of AGS-2 (+strand): . . . . . . 21324 TTTAAGGCGATTTTCTTCGATTCTAAGACAAACCCAGTTCAATTCTGTGTTTAATCTTCC F K A I F F D S K T N P V Q F C V - S S L R R F S S I L R Q T Q F N S V F N L P - G D F L R F - D K P S S I L C L I F . . . . . . 21384 TGTTTCGTCTTTCTCTAAGCCGATCCGAACAGTTTTACCCAATTCTTTGGTTACCTCTAA C F V F L - A D P N S F T Q F F G Y L - V S S F S K P I R T V L P N S L V T S K L F R L S L S R S E Q F Y P I L W L P L . . . . . . 21444 GGGTTTTGAGTTGTGGGGTTTAAGATCAGATCATACCATGGCTAGTCAATCAAGCAACCC G F - V V G F K I R S Y H G - S I K Q P G F E L W G L R S D H T M A S Q S S N P R V L S C G V - D Q I I P W L V N Q A T . . . : . . . 21504 TCAATCTGTTTATGATTTCACTGTCAAG : GATGCTAAGGGCAAGGACGTTGATCTCAGTAT S I C L - F H C Q : G C - G Q G R - S Q Y Q S V Y D F T V K : D A K G K D V D L S I L N L F M I S L S R : M L R A R T L I S V . . . . . . 22532 TTACAAGGGAAAAGTCCTTATCATTGTCAATGTTGCATCACAGTGGTATGTATTAGTTTC L Q G K S P Y H C Q C C I T V V C I S F Y K G K V L I I V N V A S Q W Y V L V S F T R E K S L S L S M L H H S G M Y - F . . . . . . 22592 GTAGTTATTTTATTCATTATATGTTTGAAGCCTCTTCACAATATTTTGGATTTCAATAGG V V I L F I I C L K P L H N I L D F N R - L F Y S L Y V - S L F T I F W I S I G R S Y F I H Y M F E A S S Q Y F G F Q - . . . . . . 22652 AACTTTCGATGAGAGAAATGATCTGATCGCTAGTTTTTGCTCCTTGTAATTAAGTGAAAT N F R - E K - S D R - F L L L V I K - N T F D E R N D L I A S F C S L - L S E I E L S M R E M I - S L V F A P C N - V K . . . . . . 22712 TTTCATGTGTTGCTCTCGATAGAATTGACTCAAAGTAAAGAAGTATTAAACTTGAACCTT F H V L L S I E L T Q S K E V L N L N L F M C C S R - N - L K V K K Y - T - T L F S C V A L D R I D S K - R S I K L E P . . . . . . 22772 ATGCTACATCAGATAGGGCATTCCTCCCCGTGATGAGAATACTATGCATTGTGATAATAA M L H Q I G H S S P - - E Y Y A L - - - C Y I R - G I P P R D E N T M H C D N N Y A T S D R A F L P V M R I L C I V I I . . . . . . 22832 CTGAATTTACGAGATATTTAGTGAAGTTTTCAGGTGTTGTCCTCCATAGAATTGACTGGA L N L R D I - - S F Q V L S S I E L T G - I Y E I F S E V F R C C P P - N - L E T E F T R Y L V K F S G V V L H R I D W . . . . 22892 AGTAAAAAAGTATTAAAATTGAACCTTATCTTACACT S K K V L K L N L I L H V K K Y - N - T L S Y T K - K S I K I E P Y L T Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-4_AGS-2_PPS_1 (21325 21531,22500 22595) (frame '2'; 300 bp, 100 residues) 1 LRRFSSILRQ TQFNSVFNLP VSSFSKPIRT VLPNSLVTSK GFELWGLRSD HTMASQSSNP 61 QSVYDFTVKD AKGKDVDLSI YKGKVLIIVN VASQWYVLVS - AGS-3 (23130 23319,23469 23587,23702 23869,23970 24171) SCR (e 1.000 d 0.000 a 1.000,e 1.000 d 0.952 a 0.998,e 1.000 d 1.000 a 0.984,e 1.000) Exon 1 23130 23319 ( 190 n); score: 1.000 Intron 1 23320 23468 ( 149 n); Pd: 0.000 Pa: 1.000 Exon 2 23469 23587 ( 119 n); score: 1.000 Intron 2 23588 23701 ( 114 n); Pd: 0.952 Pa: 0.998 Exon 3 23702 23869 ( 168 n); score: 1.000 Intron 3 23870 23969 ( 100 n); Pd: 1.000 Pa: 0.984 Exon 4 23970 24171 ( 202 n); score: 1.000 PGS (23130 23319,23469 23587,23702 23869,23970 24171) SGN-E371608- PGS (23217 23319,23469 23587,23702 23869,23970 24171) SGN-E280324- 3-phase translation of AGS-3 (+strand): . . . . . . 23130 CGGTATAATTTGATTTCATTGTGTTTACTGTGAAAGGTTTGAGCAGTTACTTGTTGTATT R Y N L I S L C L L - K V - A V T C C I G I I - F H C V Y C E R F E Q L L V V F V - F D F I V F T V K G L S S Y L L Y . . . . . . 23190 TATGATATCATTCTTTGATCTTGTTACATTTGCTTATGACTGTTAACTGATTCCATTTTC Y D I I L - S C Y I C L - L L T D S I F M I S F F D L V T F A Y D C - L I P F S L - Y H S L I L L H L L M T V N - F H F . . . . . . 23250 TGATGCAGTGGCCTGACTAATTCGAACTACACTGACATGACGGAGTTATACAAGAAGTAC - C S G L T N S N Y T D M T E L Y K K Y D A V A - L I R T T L T - R S Y T R S T L M Q W P D - F E L H - H D G V I Q E V . : . . . . . 23310 AAGGATCAAG : GTTTGGAGATTCTGGCCTTCCCTTGCAACCAGTTTGGTGGACAGGAGCCT K D Q : G L E I L A F P C N Q F G G Q E P R I K : V W R F W P S L A T S L V D R S L Q G S R : F G D S G L P L Q P V W W T G A . . . . . . 23519 GGAAACATTGAAGATATCCAGCAAATGGTCTGCACTCGCTTCAAGGCTGAGTACCCCATA G N I E D I Q Q M V C T R F K A E Y P I E T L K I S S K W S A L A S R L S T P Y W K H - R Y P A N G L H S L Q G - V P H . : . . . . . 23579 TTTGACAAG : GTTGACGTGAATGGTGATAATGCTGCTCCATTATATAGGTTTCTGAAATCA F D K : V D V N G D N A A P L Y R F L K S L T R : L T - M V I M L L H Y I G F - N Q I - Q : G - R E W - - C C S I I - V S E I . . . . . . 23753 AGCAAAGGTGGGTTCTTTGGAGATGGTATCAAGTGGAACTTCTCCAAATTCCTCATTGAC S K G G F F G D G I K W N F S K F L I D A K V G S L E M V S S G T S P N S S L T K Q R W V L W R W Y Q V E L L Q I P H - . . . . . . : 23813 AAAGAAGGACATGTCGTTGATCGCTACTCTCCAACCACTTCTCCAGCTAGCATGGAG : AAG K E G H V V D R Y S P T T S P A S M E : K K K D M S L I A T L Q P L L Q L A W R : R Q R R T C R - S L L S N H F S S - H G : E . . . . . . 23973 GATATCAAGAAACTCTTGGGTGTTGCTTAAGCTCTAGAGTCTGGACTCGGCGGTGCTGTC D I K K L L G V A - A L E S G L G G A V I S R N S W V L L K L - S L D S A V L S G Y Q E T L G C C L S S R V W T R R C C . . . . . . 24033 TCTGCATTTTGTGCTAGAAATAATGAACATATCATTGCGAATAAGCTATGTTTGATGTAA S A F C A R N N E H I I A N K L C L M - L H F V L E I M N I S L R I S Y V - C N L C I L C - K - - T Y H C E - A M F D V . . . . . . 24093 TTTTCTTCTTGTTTTAGTTCCTACAGATGTTTGCTACTTTGTACAATTTCAATATACTGA F S S C F S S Y R C L L L C T I S I Y - F L L V L V P T D V C Y F V Q F Q Y T D I F F L F - F L Q M F A T L Y N F N I L . . 24153 TCAATATTTCTATTTACAA S I F L F T Q Y F Y L Q I N I S I Y Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-4_AGS-3_PPS_1 (23253 23319,23469 23587,23702 23869,23970 24002) (frame '1'; 384 bp, 128 residues) 1 CSGLTNSNYT DMTELYKKYK DQGLEILAFP CNQFGGQEPG NIEDIQQMVC TRFKAEYPIF 61 DKVDVNGDNA APLYRFLKSS KGGFFGDGIK WNFSKFLIDK EGHVVDRYSP TTSPASMEKD 121 IKKLLGVA- PGL 5 (- strand): 30301 29838 AGS-1 (30301 29838) SCR (e 0.973) Exon 1 30301 29838 ( 464 n); score: 0.973 PGS (30301 29838) SGN-E310017+ 3-phase translation of AGS-1 (-strand): . . . . . . 30301 TTTTCTTTTCACTTTTCCTGTTTCATACAGTTTTTTGCTTACTTTTTCACTTAAAATTAC F S F H F S C F I Q F F A Y F F T - N Y F L F T F P V S Y S F L L T F S L K I T F F S L F L F H T V F C L L F H L K L . . . . . . 30241 TATCTTGGTGGTGTTTTTGTACTATAAATCCTTGGCATTTGTCAAAGCAAAGGTTAAATG Y L G G V F V L - I L G I C Q S K G - M I L V V F L Y Y K S L A F V K A K V K C L S W W C F C T I N P W H L S K Q R L N . . . . . . 30181 TGTGATCTTTAGAACTTCTGTTGGTTCAAAATTCAATCTTTATGTATTTTCAAGATTTCA C D L - N F C W F K I Q S L C I F K I S V I F R T S V G S K F N L Y V F S R F H V - S L E L L L V Q N S I F M Y F Q D F . . . . . . 30121 TTTTTTTTTCTAGTTTTTAGCTTGTTAATGAACTTACTGAAAAACCCATCTTAAACCCTT F F F L V F S L L M N L L K N P S - T L F F F - F L A C - - T Y - K T H L K P F I F F S S F - L V N E L T E K P I L N P . . . . . . 30061 CTGAGGATTTTAAGAAAAGTTGAATCTTTTTCATGGTTTGATTAGATTTCTGTTGTTGGG L R I L R K V E S F S W F D - I S V V G - G F - E K L N L F H G L I R F L L L G S E D F K K S - I F F M V - L D F C C W . . . . . . 30001 TTTTGTGTTCCATTTTGAATTGGTTGATTGAACTTCAAAAAAGTTATCACCTTTAGTACA F C V P F - I G - L N F K K V I T F S T F V F H F E L V D - T S K K L S P L V Q V L C S I L N W L I E L Q K S Y H L - Y . . . . . . 29941 ATAGTAGAATCAAGACTCAGTTTTTCTTCTACTTTTGTTGTTGGGTTTTATGTTATTGTT I V E S R L S F S S T F V V G F Y V I V - - N Q D S V F L L L L L L G F M L L F N S R I K T Q F F F Y F C C W V L C Y C . . . . . 29881 TGTACTTTATGTTCCATTTTGAATTGGTTGATTGAACTACATAA C T L C S I L N W L I E L H V L Y V P F - I G - L N Y I L Y F M F H F E L V D - T T - Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (+strand): . . . . . . 29838 TTATGTAGTTCAATCAACCAATTCAAAATGGAACATAAAGTACAAACAATAACATAAAAC L C S S I N Q F K M E H K V Q T I T - N Y V V Q S T N S K W N I K Y K Q - H K T M - F N Q P I Q N G T - S T N N N I K . . . . . . 29898 CCAACAACAAAAGTAGAAGAAAAACTGAGTCTTGATTCTACTATTGTACTAAAGGTGATA P T T K V E E K L S L D S T I V L K V I Q Q Q K - K K N - V L I L L L Y - R - - P N N K S R R K T E S - F Y Y C T K G D . . . . . . 29958 ACTTTTTTGAAGTTCAATCAACCAATTCAAAATGGAACACAAAACCCAACAACAGAAATC T F L K F N Q P I Q N G T Q N P T T E I L F - S S I N Q F K M E H K T Q Q Q K S N F F E V Q S T N S K W N T K P N N R N . . . . . . 30018 TAATCAAACCATGAAAAAGATTCAACTTTTCTTAAAATCCTCAGAAGGGTTTAAGATGGG - S N H E K D S T F L K I L R R V - D G N Q T M K K I Q L F L K S S E G F K M G L I K P - K R F N F S - N P Q K G L R W . . . . . . 30078 TTTTTCAGTAAGTTCATTAACAAGCTAAAAACTAGAAAAAAAAATGAAATCTTGAAAATA F F S K F I N K L K T R K K N E I L K I F S V S S L T S - K L E K K M K S - K Y V F Q - V H - Q A K N - K K K - N L E N . . . . . . 30138 CATAAAGATTGAATTTTGAACCAACAGAAGTTCTAAAGATCACACATTTAACCTTTGCTT H K D - I L N Q Q K F - R S H I - P L L I K I E F - T N R S S K D H T F N L C F T - R L N F E P T E V L K I T H L T F A . . . . . . 30198 TGACAAATGCCAAGGATTTATAGTACAAAAACACCACCAAGATAGTAATTTTAAGTGAAA - Q M P R I Y S T K T P P R - - F - V K D K C Q G F I V Q K H H Q D S N F K - K L T N A K D L - Y K N T T K I V I L S E . . . . . 30258 AAGTAAGCAAAAAACTGTATGAAACAGGAAAAGTGAAAAGAAAA K - A K N C M K Q E K - K E S K Q K T V - N R K S E K K K V S K K L Y E T G K V K R K Maximal non-overlapping open reading frames (>= 64 codons): none PGL 6 (- strand): 33143 32965 AGS-1 (33143 32965) SCR (e 1.000) Exon 1 33143 32965 ( 179 n); score: 1.000 PGS (33143 32965) SGN-E578101+ 3-phase translation of AGS-1 (-strand): . . . . . . 33143 CTTTCATTTATCAGTTAAATGTTTTAGTATTGTAACTTTCGAATTTTTCGAAATGTTTTG L S F I S - M F - Y C N F R I F R N V L F H L S V K C F S I V T F E F F E M F C F I Y Q L N V L V L - L S N F S K C F . . . . . . 33083 TTTAGACATTCGCTACTGTTTTATTTAATTTAAAGACTTGGAGTTATATTGTTGTGCTTT F R H S L L F Y L I - R L G V I L L C F L D I R Y C F I - F K D L E L Y C C A F V - T F A T V L F N L K T W S Y I V V L . . . . . . 33023 TATAATTTTTGTCTAGATTTATTTAGATATTGCATGAGTTAGAATGTTAAATGTTGATT Y N F C L D L F R Y C M S - N V K C - I I F V - I Y L D I A - V R M L N V D L - F L S R F I - I L H E L E C - M L I Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (+strand): . . . . . . 32965 AATCAACATTTAACATTCTAACTCATGCAATATCTAAATAAATCTAGACAAAAATTATAA N Q H L T F - L M Q Y L N K S R Q K L - I N I - H S N S C N I - I N L D K N Y K S T F N I L T H A I S K - I - T K I I . . . . . . 33025 AAGCACAACAATATAACTCCAAGTCTTTAAATTAAATAAAACAGTAGCGAATGTCTAAAC K H N N I T P S L - I K - N S S E C L N S T T I - L Q V F K L N K T V A N V - T K A Q Q Y N S K S L N - I K Q - R M S K . . . . . . 33085 AAAACATTTCGAAAAATTCGAAAGTTACAATACTAAAACATTTAACTGATAAATGAAAG K T F R K I R K L Q Y - N I - L I N E K H F E K F E S Y N T K T F N - - M K Q N I S K N S K V T I L K H L T D K - K Maximal non-overlapping open reading frames (>= 64 codons): none PGL 7 (+ strand): 38254 38739 AGS-1 (38254 38739) SCR (e 0.938) Exon 1 38254 38739 ( 486 n); score: 0.938 PGS (38254 38739) SGN-E318680- 3-phase translation of AGS-1 (+strand): . . . . . . 38254 AGACATAGGAACCAATCTATCATACTCTACCCATAGCTCCCTCAATCGAGAAAAATATAC R H R N Q S I I L Y P - L P Q S R K I Y D I G T N L S Y S T H S S L N R E K Y T T - E P I Y H T L P I A P S I E K N I . . . . . . 38314 AGATATAGTGCTCATACCTTGACTGATTGTAGCAATTTCTTTGTGTAATTGATAAATTCA R Y S A H T L T D C S N F F V - L I N S D I V L I P - L I V A I S L C N - - I Q Q I - C S Y L D - L - Q F L C V I D K F . . . . . . 38374 ACTCCCATCCACTTTATCATATCTTTCACACAGTTCCTTCCACACTTCTGCTGCATTAGT T P I H F I I S F T Q F L P H F C C I S L P S T L S Y L S H S S F H T S A A L V N S H P L Y H I F H T V P S T L L L H - . . . . . . 38434 TGAGTAAACAATTCCACTAAGCAACTCTTTTGATACACAATTCATAATCCATGATAGAAC - V N N S T K Q L F - Y T I H N P - - N E - T I P L S N S F D T Q F I I H D R T L S K Q F H - A T L L I H N S - S M I E . . . . . . 38494 AATGGCATTACATCGTTCCCACAGATTCTTCAAGTTTTATCCATATGCTTCATTCTTGCA N G I T S F P Q I L Q V L S I C F I L A M A L H R S H R F F K F Y P Y A S F L Q Q W H Y I V P T D S S S F I H M L H S C . . . . . . 38554 AGATCCATCGATGAATCCTAGCTTATTTCTTCCTAGAATAGCTATTTTCATAGCTCTGCT R S I D E S - L I S S - N S Y F H S S A D P S M N P S L F L P R I A I F I A L L K I H R - I L A Y F F L E - L F S - L C . . . . . . 38614 CCACACTGAGAAGTTTTCTAATCCAGATAGCTGGATCGAAATCAACATAACACCTGAGTT P H - E V F - S R - L D R N Q H N T - V H T E K F S N P D S W I E I N I T P E L S T L R S F L I Q I A G S K S T - H L S . . . . . . 38674 ATCGATGTTATTCAAGAACAATGGATGATTATGACTCAATTTTTCTGGAAGTTCATTATC I D V I Q E Q W M I M T Q F F W K F I I S M L F K N N G - L - L N F S G S S L S Y R C Y S R T M D D Y D S I F L E V H Y . 38734 AACGAC N D T Q R Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-7_AGS-1_PPS_1 (38441 38701) (frame '2'; 258 bp, 86 residues) 1 TIPLSNSFDT QFIIHDRTMA LHRSHRFFKF YPYASFLQDP SMNPSLFLPR IAIFIALLHT 61 EKFSNPDSWI EINITPELSM LFKNNG- 3-phase translation of AGS-1 (-strand): . . . . . . 38739 GTCGTTGATAATGAACTTCCAGAAAAATTGAGTCATAATCATCCATTGTTCTTGAATAAC V V D N E L P E K L S H N H P L F L N N S L I M N F Q K N - V I I I H C S - I T R - - - T S R K I E S - S S I V L E - . . . . . . 38679 ATCGATAACTCAGGTGTTATGTTGATTTCGATCCAGCTATCTGGATTAGAAAACTTCTCA I D N S G V M L I S I Q L S G L E N F S S I T Q V L C - F R S S Y L D - K T S Q H R - L R C Y V D F D P A I W I R K L L . . . . . . 38619 GTGTGGAGCAGAGCTATGAAAATAGCTATTCTAGGAAGAAATAAGCTAGGATTCATCGAT V W S R A M K I A I L G R N K L G F I D C G A E L - K - L F - E E I S - D S S M S V E Q S Y E N S Y S R K K - A R I H R . . . . . . 38559 GGATCTTGCAAGAATGAAGCATATGGATAAAACTTGAAGAATCTGTGGGAACGATGTAAT G S C K N E A Y G - N L K N L W E R C N D L A R M K H M D K T - R I C G N D V M W I L Q E - S I W I K L E E S V G T M - . . . . . . 38499 GCCATTGTTCTATCATGGATTATGAATTGTGTATCAAAAGAGTTGCTTAGTGGAATTGTT A I V L S W I M N C V S K E L L S G I V P L F Y H G L - I V Y Q K S C L V E L F C H C S I M D Y E L C I K R V A - W N C . . . . . . 38439 TACTCAACTAATGCAGCAGAAGTGTGGAAGGAACTGTGTGAAAGATATGATAAAGTGGAT Y S T N A A E V W K E L C E R Y D K V D T Q L M Q Q K C G R N C V K D M I K W M L L N - C S R S V E G T V - K I - - S G . . . . . . 38379 GGGAGTTGAATTTATCAATTACACAAAGAAATTGCTACAATCAGTCAAGGTATGAGCACT G S - I Y Q L H K E I A T I S Q G M S T G V E F I N Y T K K L L Q S V K V - A L W E L N L S I T Q R N C Y N Q S R Y E H . . . . . . 38319 ATATCTGTATATTTTTCTCGATTGAGGGAGCTATGGGTAGAGTATGATAGATTGGTTCCT I S V Y F S R L R E L W V E Y D R L V P Y L Y I F L D - G S Y G - S M I D W F L Y I C I F F S I E G A M G R V - - I G S . 38259 ATGTCT M S C Y V Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-7_AGS-1_PPS_1 (38739 38530) (frame '1'; 207 bp, 69 residues) 1 VVDNELPEKL SHNHPLFLNN IDNSGVMLIS IQLSGLENFS VWSRAMKIAI LGRNKLGFID 61 GSCKNEAYG- PGL 8 (- strand): 45032 43729 AGS-1 (45032 44776,44562 44518,44436 44400,44093 43729) SCR (e 1.000 d 1.000 a 0.000,e 0.489 d 0.878 a 0.840,e 0.568 d 0.308 a 0.000,e 0.871) Exon 1 45032 44776 ( 257 n); score: 1.000 Intron 1 44775 44563 ( 213 n); Pd: 1.000 Pa: 0.000 Exon 2 44562 44518 ( 45 n); score: 0.489 Intron 2 44517 44437 ( 81 n); Pd: 0.878 Pa: 0.840 Exon 3 44436 44400 ( 37 n); score: 0.568 Intron 3 44399 44094 ( 306 n); Pd: 0.308 Pa: 0.000 Exon 4 44093 43729 ( 365 n); score: 0.871 PGS (45032 44776,44562 44518,44436 44400,44093 43729) SGN-E314730+ PGS (45032 44776,44562 44518,44436 44400,44093 43884) SGN-E314578+ 3-phase translation of AGS-1 (-strand): . . . . . . 45032 TTTTTTCCCCATCTTTGAACTCCAAAAATTATAATTTACGTTCGAATTGGCTGACTCGAA F F P H L - T P K I I I Y V R I G - L E F F P I F E L Q K L - F T F E L A D S N F S P S L N S K N Y N L R S N W L T R . . . . . . 44972 TCTACAATCTATCGACTAAAAAAAAATCTCAACAATGACAGATAAAAATTATTTTTCAGA S T I Y R L K K N L N N D R - K L F F R L Q S I D - K K I S T M T D K N Y F S E I Y N L S T K K K S Q Q - Q I K I I F Q . . . . . . 44912 AATACCCCCTGGATTTACCAAATGTGTGCCTAGAAACATTGAAACTCCAACAATTTATTC N T P W I Y Q M C A - K H - N S N N L F I P P G F T K C V P R N I E T P T I Y S K Y P L D L P N V C L E T L K L Q Q F I . . . . . . 44852 TCGTCAGAGTGAGAAATTTATCGATGATTTCGAAAGAGGTAGACAACAACTCCCTCAGAT S S E - E I Y R - F R K R - T T T P S D R Q S E K F I D D F E R G R Q Q L P Q I L V R V R N L S M I S K E V D N N S L R . . : . . . . 44792 ACCAGTTTCAGCAGAAG : GAACTTTAATCGAAATTTCAGAGACATAGACATAAAAATTATT T S F S R R : N F N R N F R D I D I K I I P V S A E : G T L I E I S E T - T - K L L Y Q F Q Q K : E L - S K F Q R H R H K N Y . : . . . : . . 44519 AA : TTGAGACGCCTGAAATATATTTGAAAGTAAAAAAAAT : ATCAAAGATGAAAAGATAAAT N : - D A - N I F E S K K N : I K D E K I N : I E T P E I Y L K V K K I : S K M K R - I - : L R R L K Y I - K - K K : Y Q R - K D K . . . . . . 44072 CTGAATCCGTTGACATTACTGAGTCCACCACCAAATCAACGTGTTCGGATTAATGATTTT L N P L T L L S P P P N Q R V R I N D F - I R - H Y - V H H Q I N V F G L M I F S E S V D I T E S T T K S T C S D - - F . . . . . . 44012 TCATCTCCTCACATCAACGAGGAATCGAAGACTATAATCTCTCAGGTCAAATCAGTTTTG S S P H I N E E S K T I I S Q V K S V L H L L T S T R N R R L - S L R S N Q F W F I S S H Q R G I E D Y N L S G Q I S F . . . . . . 43952 GTGTTCCAGCTCACCTCTGGAAGCACGAAAAACATTGAAGCAATGATTAAGTTCGCGAAC V F Q L T S G S T K N I E A M I K F A N C S S S P L E A R K T L K Q - L S S R T G V P A H L W K H E K H - S N D - V R E . . . . . . 43892 AATGCATTCTTCAACTTGGATTCATCTTTCTACAACAATGTTAGAGATTTCATCGCGTGT N A F F N L D S S F Y N N V R D F I A C M H S S T W I H L S T T M L E I S S R V Q C I L Q L G F I F L Q Q C - R F H R V . . . . . . 43832 CATTATGATTTGCTCATCGCTAAGAGACAGAGAGAAATGCAGTCATTTCCTATAGAGCTG H Y D L L I A K R Q R E M Q S F P I E L I M I C S S L R D R E K C S H F L - S - S L - F A H R - E T E R N A V I S Y R A . . . . . 43772 AAGACTAGATATGAAAACGCGATAACTTGTGCAAATGATCTTAA K T R Y E N A I T C A N D L R L D M K T R - L V Q M I L E D - I - K R D N L C K - S - Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-8_AGS-1_PPS_1 (44423 44400,44093 43731) (frame '1'; 387 bp, 129 residues) 1 NIFESKKNIK DEKINLNPLT LLSPPPNQRV RINDFSSPHI NEESKTIISQ VKSVLVFQLT 61 SGSTKNIEAM IKFANNAFFN LDSSFYNNVR DFIACHYDLL IAKRQREMQS FPIELKTRYE 121 NAITCANDL >C08HBa0251H04.1-_PGL-8_AGS-1_PPS_2 (44953 44776,44562 44534) (frame '2'; 204 bp, 68 residues) 1 KKISTMTDKN YFSEIPPGFT KCVPRNIETP TIYSRQSEKF IDDFERGRQQ LPQIPVSAEG 61 TLIEISET- PGL 9 (- strand): 49958 49593 AGS-1 (49958 49593) SCR (e 0.995) Exon 1 49958 49593 ( 366 n); score: 0.995 PGS (49958 49593) SGN-E239441+ 3-phase translation of AGS-1 (-strand): . . . . . . 49958 TTTTTTTCGTGTTTCTACAAACCACCAAACCATTAACTTTTTTTTATCATTAGCTCCATT F F S C F Y K P P N H - L F F I I S S I F F R V S T N H Q T I N F F L S L A P F F F V F L Q T T K P L T F F Y H - L H . . . . . . 49898 TTTAATCACTCTTGAACCTCTTTGAACTAATTTTTCATGTATTTTGAGAATCCGCAGCAG F N H S - T S L N - F F M Y F E N P Q Q L I T L E P L - T N F S C I L R I R S R F - S L L N L F E L I F H V F - E S A A . . . . . . 49838 AAGGAAGCTAATCAATGTCAAAAACAATTGAAATTCCACCTGGGTTTACAAAAACATTGT K E A N Q C Q K Q L K F H L G L Q K H C R K L I N V K N N - N S T W V Y K N I V E G S - S M S K T I E I P P G F T K T L . . . . . . 49778 GCAAAAGAATAAGTATTGATCACGATAGTTACATGCAAGATGATAAGAGGAAAATTGGGT A K E - V L I T I V T C K M I R G K L G Q K N K Y - S R - L H A R - - E E N W V C K R I S I D H D S Y M Q D D K R K I G . . . . . . 49718 CTGTTAATGAACTTTCTGGTAGAAGGTGCAGTTTAAGAGAAAGATTTGCCCCAAATCTGT L L M N F L V E G A V - E K D L P Q I C C - - T F W - K V Q F K R K I C P K S V S V N E L S G R R C S L R E R F A P N L . . . . . . 49658 ATATTCCTGTCCAGTTTCAGCCCCGTCCACGTACACCGGGCCGCCCTCGGAAACAGAGAT I F L S S F S P V H V H R A A L G N R D Y S C P V S A P S T Y T G P P S E T E I Y I P V Q F Q P R P R T P G R P R K Q R . 49598 CAGATG Q M R S D Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-9_AGS-1_PPS_1 (49827 49594) (frame '0'; 234 bp, 78 residues) 1 SMSKTIEIPP GFTKTLCKRI SIDHDSYMQD DKRKIGSVNE LSGRRCSLRE RFAPNLYIPV 61 QFQPRPRTPG RPRKQRSD 3-phase translation of AGS-1 (+strand): . . . . . . 49593 CATCTGATCTCTGTTTCCGAGGGCGGCCCGGTGTACGTGGACGGGGCTGAAACTGGACAG H L I S V S E G G P V Y V D G A E T G Q I - S L F P R A A R C T W T G L K L D R S D L C F R G R P G V R G R G - N W T . . . . . . 49653 GAATATACAGATTTGGGGCAAATCTTTCTCTTAAACTGCACCTTCTACCAGAAAGTTCAT E Y T D L G Q I F L L N C T F Y Q K V H N I Q I W G K S F S - T A P S T R K F I G I Y R F G A N L S L K L H L L P E S S . . . . . . 49713 TAACAGACCCAATTTTCCTCTTATCATCTTGCATGTAACTATCGTGATCAATACTTATTC - Q T Q F S S Y H L A C N Y R D Q Y L F N R P N F P L I I L H V T I V I N T Y S L T D P I F L L S S C M - L S - S I L I . . . . . . 49773 TTTTGCACAATGTTTTTGTAAACCCAGGTGGAATTTCAATTGTTTTTGACATTGATTAGC F C T M F L - T Q V E F Q L F L T L I S F A Q C F C K P R W N F N C F - H - L A L L H N V F V N P G G I S I V F D I D - . . . . . . 49833 TTCCTTCTGCTGCGGATTCTCAAAATACATGAAAAATTAGTTCAAAGAGGTTCAAGAGTG F L L L R I L K I H E K L V Q R G S R V S F C C G F S K Y M K N - F K E V Q E - L P S A A D S Q N T - K I S S K R F K S . . . . . . 49893 ATTAAAAATGGAGCTAATGATAAAAAAAAGTTAATGGTTTGGTGGTTTGTAGAAACACGA I K N G A N D K K K L M V W W F V E T R L K M E L M I K K S - W F G G L - K H E D - K W S - - - K K V N G L V V C R N T . 49953 AAAAAA K K K K K Maximal non-overlapping open reading frames (>= 64 codons): none PGL 10 (- strand): 60161 53740 AGS-1 (60161 59976,59149 58970,58847 58786,57724 57646,56852 56706,56600 56467,54862 54742,54658 54585,53880 53740) SCR (e 1.000 d 0.991 a 0.997,e 1.000 d 0.969 a 0.994,e 1.000 d 0.994 a 0.994,e 1.000 d 0.985 a 0.956,e 1.000 d 0.992 a 0.919,e 1.000 d 1.000 a 0.991,e 1.000 d 0.900 a 0.998,e 1.000 d 0.949 a 0.950,e 1.000) Exon 1 60161 59976 ( 186 n); score: 1.000 Intron 1 59975 59150 ( 826 n); Pd: 0.991 Pa: 0.997 Exon 2 59149 58970 ( 180 n); score: 1.000 Intron 2 58969 58848 ( 122 n); Pd: 0.969 Pa: 0.994 Exon 3 58847 58786 ( 62 n); score: 1.000 Intron 3 58785 57725 (1061 n); Pd: 0.994 Pa: 0.994 Exon 4 57724 57646 ( 79 n); score: 1.000 Intron 4 57645 56853 ( 793 n); Pd: 0.985 Pa: 0.956 Exon 5 56852 56706 ( 147 n); score: 1.000 Intron 5 56705 56601 ( 105 n); Pd: 0.992 Pa: 0.919 Exon 6 56600 56467 ( 134 n); score: 1.000 Intron 6 56466 54863 (1604 n); Pd: 1.000 Pa: 0.991 Exon 7 54862 54742 ( 121 n); score: 1.000 Intron 7 54741 54659 ( 83 n); Pd: 0.900 Pa: 0.998 Exon 8 54658 54585 ( 74 n); score: 1.000 Intron 8 54584 53881 ( 704 n); Pd: 0.949 Pa: 0.950 Exon 9 53880 53740 ( 141 n); score: 1.000 PGS (56815 56706,56600 56467,54862 54742,54658 54585,53880 53740) SGN-E369675+ PGS (56815 56706,56600 56467,54862 54742,54658 54585,53880 53752) SGN-E259277+ PGS (56815 56706,56600 56467,54862 54742,54658 54585,53880 53755) SGN-E252039+ PGS (56812 56706,56600 56467,54862 54742,54658 54585,53880 53818) SGN-E255440+ PGS (59122 58970,58847 58786,57724 57646,56852 56706,56600 56470) SGN-E302151+ PGS (60161 59976,59149 58970,58847 58786,57724 57646,56852 56829) SGN-E205815+ 3-phase translation of AGS-1 (-strand): . . . . . . 60161 GATGATGAAATATTGAGCAGATTTGGTAAAATTGAAAAGCAAATTCAAGCCATAGAATCA D D E I L S R F G K I E K Q I Q A I E S M M K Y - A D L V K L K S K F K P - N Q - - N I E Q I W - N - K A N S S H R I . . . . . . 60101 GTTGTAGAAGCAGGTCAAGGTCTTGGTTTCAAGTTTGATATGGTTCCAAAACTTGAAGAT V V E A G Q G L G F K F D M V P K L E D L - K Q V K V L V S S L I W F Q N L K M S C R S R S R S W F Q V - Y G S K T - R . . . . . . 60041 GCTTGTTCTACTCTTCGTTGGTGCTTTAGGGCTCTATCTTTTGCTACTGCAATTCCAACA A C S T L R W C F R A L S F A T A I P T L V L L F V G A L G L Y L L L L Q F Q H C L F Y S S L V L - G S I F C Y C N S N . : . . . . . 59981 CTTGAG : GAAGTCAAGACGAATTTGGAGATTGCGACCCACCTTCCTATCATGTATACAACT L E : E V K T N L E I A T H L P I M Y T T L R : K S R R I W R L R P T F L S C I Q L T - : G S Q D E F G D C D P P S Y H V Y N . . . . . . 59095 TGCAGTTTGTGTATCTCCTTACTTGATTGGGTGAATTGGCTTAATAGAGCTTTGGAAGTC C S L C I S L L D W V N W L N R A L E V A V C V S P Y L I G - I G L I E L W K S L Q F V Y L L T - L G E L A - - S F G S . . . . . . 59035 TCCATTCTATCTACTGCTGGAAGATCCAACTTAAGTGATGCTGAAGAAGTTCTCAGACAG S I L S T A G R S N L S D A E E V L R Q P F Y L L L E D P T - V M L K K F S D S L H S I Y C W K I Q L K - C - R S S Q T . : . . . . . 58975 TATCAG : AACATTTGTGTCTCATCTCCTGCAATGATTAGTCAGCTTCAGAAGGCTATTGAG Y Q : N I C V S S P A M I S Q L Q K A I E I R : T F V S H L L Q - L V S F R R L L R V S : E H L C L I S C N D - S A S E G Y - . : . . . . . 58793 AAACACAA : CTCATGGATGGATCAGGTGCATTCATTTTTTGTCCTTAACTTTAGGGATCGA K H N : S W M D Q V H S F F V L N F R D R N T : T H G W I R C I H F L S L T L G I D E T Q : L M D G S G A F I F C P - L - G S . . . : . . . 57672 TCATGGGATCTGTTGCTGCAGCTTAAG : GAAAAAGGGAATAATGATGCTTTCAGTTGCTCC S W D L L L Q L K : E K G N N D A F S C S H G I C C C S L R : K K G I M M L S V A P I M G S V A A A - : G K R E - - C F Q L L . . . . . . 56819 GAATTGGACATGGTTTTCTCTGAGGTTCATAAGACAGATGAGTGGAAGCGCCGGTGTGAG E L D M V F S E V H K T D E W K R R C E N W T W F S L R F I R Q M S G S A G V R R I G H G F L - G S - D R - V E A P V - . . . . . . : 56759 GAGGTCTTACACCCTTCAATTAGAGATGCTAACCTGCTTGCTGCTCTTTTACAG : ACTAAA E V L H P S I R D A N L L A A L L Q : T K R S Y T L Q L E M L T C L L L F Y R : L K G G L T P F N - R C - P A C C S F T : D - . . . . . . 56594 AACGCCCTTGAGAGATCAATTAACATTTGTGAGAAATCTAACCAGACCAATGCAAGCGCG N A L E R S I N I C E K S N Q T N A S A T P L R D Q L T F V R N L T R P M Q A R K R P - E I N - H L - E I - P D Q C K R . . . . . . 56534 TTATGTATATTCTGCTCACATGATGGTGTGAACCAGAAGCTTTTGACATGTTCTACTTGC L C I F C S H D G V N Q K L L T C S T C Y V Y S A H M M V - T R S F - H V L L A V M Y I L L T - W C E P E A F D M F Y L . : . . . . . 56474 AATGACAG : CTTCCACTTGAAGTGCATAGGATGGTCACCAGGAGATGCCAATGATTCGAAA N D S : F H L K C I G W S P G D A N D S K M T : A S T - S A - D G H Q E M P M I R K Q - Q : L P L E V H R M V T R R C Q - F E . . . . . . 54810 GTTTTCATTTGTCCTTATTGTCACTTCATGAACAGTGGAAAAATATCAAGAAATGGAAGT V F I C P Y C H F M N S G K I S R N G S F S F V L I V T S - T V E K Y Q E M E V S F H L S L L S L H E Q W K N I K K W K . : . . . . . 54750 GATCCTCTG : AATATTGGTAGGAAGAGTTTCAAGTTGCACAAGCTCGTTGAGCTCCTATCT D P L : N I G R K S F K L H K L V E L L S I L - : I L V G R V S S C T S S L S S Y L - S S : E Y W - E E F Q V A Q A R - A P I . . . : . . . 54607 GACGCTGAAGATCTATGTTTATG : GATACAGGAAAGAGCTGTACTTCATCAAATTGGTCAA D A E D L C L W : I Q E R A V L H Q I G Q T L K I Y V Y : G Y R K E L Y F I K L V K - R - R S M F M : D T G K S C T S S N W S . . . . . . 53843 AAAGCTCTCGATTTCAAAGCTCGCATAGAAGAGATTGTCAAGTTTGTTTTAGCTTATCTA K A L D F K A R I E E I V K F V L A Y L K L S I S K L A - K R L S S L F - L I - K S S R F Q S S H R R D C Q V C F S L S . . . . . 53783 GATGAAGACCTTAGCATTATTGCAAAAAAATTCTGTGTTGCTTT D E D L S I I A K K F C V A M K T L A L L Q K N S V L L R - R P - H Y C K K I L C C F Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-10_AGS-1_PPS_1 (60161 59976,59149 58970,58847 58786,57724 57646,56852 56706,56600 56467,54862 54742,54658 54585,53880 53742) (frame '1'; 1122 bp, 374 residues) 1 DDEILSRFGK IEKQIQAIES VVEAGQGLGF KFDMVPKLED ACSTLRWCFR ALSFATAIPT 61 LEEVKTNLEI ATHLPIMYTT CSLCISLLDW VNWLNRALEV SILSTAGRSN LSDAEEVLRQ 121 YQNICVSSPA MISQLQKAIE KHNSWMDQVH SFFVLNFRDR SWDLLLQLKE KGNNDAFSCS 181 ELDMVFSEVH KTDEWKRRCE EVLHPSIRDA NLLAALLQTK NALERSINIC EKSNQTNASA 241 LCIFCSHDGV NQKLLTCSTC NDSFHLKCIG WSPGDANDSK VFICPYCHFM NSGKISRNGS 301 DPLNIGRKSF KLHKLVELLS DAEDLCLWIQ ERAVLHQIGQ KALDFKARIE EIVKFVLAYL 361 DEDLSIIAKK FCVA PGL 11 (- strand): 78242 77936 AGS-1 (78242 77936) SCR (e 1.000) Exon 1 78242 77936 ( 307 n); score: 1.000 PGS (78242 77936) SGN-E215930- 3-phase translation of AGS-1 (-strand): . . . . . . 78242 CTATCTTTGAGGAGCTTCTCTACTGAAGTTGTTTGAGAGTGCAATTTCAATTCTGTCTTT L S L R S F S T E V V - E C N F N S V F Y L - G A S L L K L F E S A I S I L S F I F E E L L Y - S C L R V Q F Q F C L . . . . . . 78182 TTCCTGTGTTGTAAATGATTTCTCAATTTCATTGAGAGTTATATCAGCCGATTTTTCCAT F L C C K - F L N F I E S Y I S R F F H S C V V N D F S I S L R V I S A D F S I F P V L - M I S Q F H - E L Y Q P I F P . . . . . . 78122 CGTTAGACAAATAATCTAGCTAGCTAGATTAAGATATGTTACATGTATCTATGGCTCGAA R - T N N L A S - I K I C Y M Y L W L E V R Q I I - L A R L R Y V T C I Y G S K S L D K - S S - L D - D M L H V S M A R . . . . . . 78062 AACGTCATATGTAGCTCTCTCCCCTTGGGATGTGATCAATCTGGTTCCAGATAGCATTTG N V I C S S L P L G C D Q S G S R - H L T S Y V A L S P W D V I N L V P D S I C K R H M - L S P L G M - S I W F Q I A F . . . . . . 78002 TATACATTTTCCCCAGAAAAGTAACATAGCAATGCTAAAGTAATAAATGAATCTATTGGC Y T F S P E K - H S N A K V I N E S I G I H F P Q K S N I A M L K - - M N L L A V Y I F P R K V T - Q C - S N K - I Y W . 77942 TGACCAA - P D Q L T Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (+strand): . . . . . . 77936 TTGGTCAGCCAATAGATTCATTTATTACTTTAGCATTGCTATGTTACTTTTCTGGGGAAA L V S Q - I H L L L - H C Y V T F L G K W S A N R F I Y Y F S I A M L L F W G K G Q P I D S F I T L A L L C Y F S G E . . . . . . 77996 ATGTATACAAATGCTATCTGGAACCAGATTGATCACATCCCAAGGGGAGAGAGCTACATA M Y T N A I W N Q I D H I P R G E S Y I C I Q M L S G T R L I T S Q G E R A T Y N V Y K C Y L E P D - S H P K G R E L H . . . . . . 78056 TGACGTTTTCGAGCCATAGATACATGTAACATATCTTAATCTAGCTAGCTAGATTATTTG - R F R A I D T C N I S - S S - L D Y L D V F E P - I H V T Y L N L A S - I I C M T F S S H R Y M - H I L I - L A R L F . . . . . . 78116 TCTAACGATGGAAAAATCGGCTGATATAACTCTCAATGAAATTGAGAAATCATTTACAAC S N D G K I G - Y N S Q - N - E I I Y N L T M E K S A D I T L N E I E K S F T T V - R W K N R L I - L S M K L R N H L Q . . . . . . 78176 ACAGGAAAAAGACAGAATTGAAATTGCACTCTCAAACAACTTCAGTAGAGAAGCTCCTCA T G K R Q N - N C T L K Q L Q - R S S S Q E K D R I E I A L S N N F S R E A P Q H R K K T E L K L H S Q T T S V E K L L . 78236 AAGATAG K I R - K D Maximal non-overlapping open reading frames (>= 64 codons): none PGL 12 (- strand): 85839 79284 AGS-1 (85839 85640,84712 84375,83672 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79614,79373 79284) SCR (e 1.000 d 0.935 a 0.993,e 1.000 d 0.992 a 0.746,e 1.000 d 0.994 a 0.994,e 1.000 d 0.987 a 0.999,e 1.000 d 0.977 a 0.707,e 1.000 d 0.999 a 0.999,e 1.000 d 1.000 a 0.986,e 1.000 d 0.998 a 0.996,e 1.000 d 1.000 a 0.982,e 1.000 d 0.939 a 0.993,e 1.000 d 0.999 a 0.991,e 1.000 d 0.993 a 0.999,e 1.000) Exon 1 85839 85640 ( 200 n); score: 1.000 Intron 1 85639 84713 ( 927 n); Pd: 0.935 Pa: 0.993 Exon 2 84712 84375 ( 338 n); score: 1.000 Intron 2 84374 83673 ( 702 n); Pd: 0.992 Pa: 0.746 Exon 3 83672 83522 ( 151 n); score: 1.000 Intron 3 83521 83329 ( 193 n); Pd: 0.994 Pa: 0.994 Exon 4 83328 83221 ( 108 n); score: 1.000 Intron 4 83220 82774 ( 447 n); Pd: 0.987 Pa: 0.999 Exon 5 82773 82681 ( 93 n); score: 1.000 Intron 5 82680 81500 (1181 n); Pd: 0.977 Pa: 0.707 Exon 6 81499 81416 ( 84 n); score: 1.000 Intron 6 81415 81204 ( 212 n); Pd: 0.999 Pa: 0.999 Exon 7 81203 81135 ( 69 n); score: 1.000 Intron 7 81134 81056 ( 79 n); Pd: 1.000 Pa: 0.986 Exon 8 81055 80985 ( 71 n); score: 1.000 Intron 8 80984 80901 ( 84 n); Pd: 0.998 Pa: 0.996 Exon 9 80900 80832 ( 69 n); score: 1.000 Intron 9 80831 80039 ( 793 n); Pd: 1.000 Pa: 0.982 Exon 10 80038 79945 ( 94 n); score: 1.000 Intron 10 79944 79874 ( 71 n); Pd: 0.939 Pa: 0.993 Exon 11 79873 79784 ( 90 n); score: 1.000 Intron 11 79783 79695 ( 89 n); Pd: 0.999 Pa: 0.991 Exon 12 79694 79614 ( 81 n); score: 1.000 Intron 12 79613 79374 ( 240 n); Pd: 0.993 Pa: 0.999 Exon 13 79373 79284 ( 90 n); score: 1.000 PGS (81467 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79614,79373 79284) SGN-E250603+ PGS (83309 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79623) SGN-E263535+ PGS (83247 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79654) SGN-E212583+ PGS (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79787) SGN-E261343+ PGS (82744 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79811) SGN-E250238+ PGS (83618 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 81050) SGN-E256709+ PGS (83620 83522,83328 83221,82773 82681,81499 81440) SGN-E270608+ PGS (83685 83522,83328 83221,82773 82681) SGN-E284559+ PGS (84710 84375,83672 83522,83328 83221,82773 82696) SGN-E339388+ PGS (84422 84375,83672 83522,83328 83221,82773 82748) SGN-E200628+ PGS (85675 85640,84712 84375,83672 83522,83328 83281) SGN-E204027+ PGS (85836 85640,84712 84375,83672 83522,83328 83311) SGN-E348164+ PGS (85839 85640,84712 84375,83672 83563) SGN-E342804+ PGS (85671 85640,84712 84375,83672 83578) SGN-E306778+ PGS (85773 85640,84712 84375,83672 83610) SGN-E207723+ 3-phase translation of AGS-1 (-strand): . . . . . . 85839 TTGTCTCTCTTTATAGCTAAACAAAAATTAAAGAAATGGGAGTGATAAAGCTCTAGCCGC L S L F I A K Q K L K K W E - - S S S R C L S L - L N K N - R N G S D K A L A A V S L Y S - T K I K E M G V I K L - P . . . . . . 85779 CTCAGCACACCAAAATCCTAATCATTTTGCTGATACCCAATTGTCTCTATATATATATTG L S T P K S - S F C - Y P I V S I Y I L S A H Q N P N H F A D T Q L S L Y I Y W P Q H T K I L I I L L I P N C L Y I Y I . . . . . . 85719 GAAGGTTTTCTTTCAATTTTCAGTCAATTCTATAGAACAAACCCTACAAAGTCTTAATTT E G F L S I F S Q F Y R T N P T K S - F K V F F Q F S V N S I E Q T L Q S L N F G R F S F N F Q S I L - N K P Y K V L I . . : . . . . 85659 TTCCAGTTTACTGAGCCAAG : GCACAAAGCATGGCCTTGATGTCTCAGGCAGGTTCTTCAC F Q F T E P R : H K A W P - C L R Q V L H S S L L S Q : G T K H G L D V S G R F F T F P V Y - A K : A Q S M A L M S Q A G S S . . . . . . 84672 ATTGCATTTACTCTGAAAAGGTAAGATGTATCAGTGGACATAGAAGCATCATTAACAATA I A F T L K R - D V S V D I E A S L T I L H L L - K G K M Y Q W T - K H H - Q Y H C I Y S E K V R C I S G H R S I I N N . . . . . . 84612 TGGATATGTTCAGAATGCGGGAGATATGCTTTGGCGTGGATATTTCTTCACGCAATGCCT W I C S E C G R Y A L A W I F L H A M P G Y V Q N A G D M L W R G Y F F T Q C L M D M F R M R E I C F G V D I S S R N A . . . . . . 84552 CAAGACGAGTGCAAGGGAATTATTTGAATCACATAGGAGTGGGAAGTAGACGTGGAGACC Q D E C K G I I - I T - E W E V D V E T K T S A R E L F E S H R S G K - T W R P S R R V Q G N Y L N H I G V G S R R G D . . . . . . 84492 TGACAATTGTAGCTGCAAGTCCTCCAACAGAGGATGCTGTTGTTGCAGCAGAGCCGTTAA - Q L - L Q V L Q Q R M L L L Q Q S R - D N C S C K S S N R G C C C C S R A V N L T I V A A S P P T E D A V V A A E P L . . . . . . : 84432 CAAAAGAAGATCTTGTAGGATATCTTGCTTCTGGATGCAAATCCAAAGAAAAGTGGAG : GA Q K K I L - D I L L L D A N P K K S G : G K R R S C R I S C F W M Q I Q R K V E : D T K E D L V G Y L A S G C K S K E K W R : . . . . . . 83670 TAGGCACTGAACATGAAAAGTTTGGTTTCGAGTTTGGAACCCTGCGACCCATGAAGTATG - A L N M K S L V S S L E P C D P - S M R H - T - K V W F R V W N P A T H E V - I G T E H E K F G F E F G T L R P M K Y . . . . . . 83610 ATCAAATAGCTGACTTGCTAAATGGTATTGCTGAGCGGTTTGATTGGGAAAAAGTAATGG I K - L T C - M V L L S G L I G K K - W S N S - L A K W Y C - A V - L G K S N G D Q I A D L L N G I A E R F D W E K V M . . . : . . . 83550 AGGGTGACAAGATTATTGGCCTGAAACAG : GGAAAGCAAAGCATATCATTAGAACCTGGTG R V T R L L A - N R : E S K A Y H - N L V G - Q D Y W P E T : G K A K H I I R T W W E G D K I I G L K Q : G K Q S I S L E P G . . . . . . 83297 GTCAGTTTGAGCTTAGTGGTGCACCACTTGAAACACTGCATCAAACTTGTGCAGAGGTTA V S L S L V V H H L K H C I K L V Q R L S V - A - W C T T - N T A S N L C R G - G Q F E L S G A P L E T L H Q T C A E V . . : . . . . 83237 ATTCACATCTTTACCAG : GTTAAAGCTGTTGCAGAAGAGATGGGAATTGGATTCTTAGGAA I H I F T R : L K L L Q K R W E L D S - E F T S L P : G - S C C R R D G N W I L R N N S H L Y Q : V K A V A E E M G I G F L G . . . . . : . 82730 CTGGATTCCAGCCAAAGTGGGGGCTGAAAGATATACCAATAATGCCGAAG : GGGAGATATG L D S S Q S G G - K I Y Q - C R R : G D M W I P A K V G A E R Y T N N A E : G E I - T G F Q P K W G L K D I P I M P K : G R Y . . . . . . 81489 AGATTATGAGAAATTACATGCCTAAAGTTGGCTCACTTGGGCTGGATATGATGTTTAGAA R L - E I T C L K L A H L G W I - C L E D Y E K L H A - S W L T W A G Y D V - N E I M R N Y M P K V G S L G L D M M F R . . : . . . . 81429 CATGCACTGTTCAG : GTAAATCTGGACTTCAGTTCTGAAGCTGACATGATCAGAAAGTTCC H A L F R : - I W T S V L K L T - S E S S M H C S : G K S G L Q F - S - H D Q K V P T C T V Q : V N L D F S S E A D M I R K F . . . : . . . 81157 GTGCTGGTCTTGCCTTGCAGCCT : ATTGCTACAGCTCTATTTGCAAATTCACCTTTCACTG V L V L P C S L : L L Q L Y L Q I H L S L C W S C L A A : Y C Y S S I C K F T F H - R A G L A L Q P : I A T A L F A N S P F T . . . . : . . 81018 AAGGAAAACCTAATGGTTATCTCAGCAAGAGAAG : CCACATTTGGACCGATACAGATAATA K E N L M V I S A R E : A T F G P I Q I I R K T - W L S Q Q E K : P H L D R Y R - - E G K P N G Y L S K R S : H I W T D T D N . . . . . : . 80874 ACCGCGCTGGGATGCTTCCTTTCGTCTTTGATGACTCTTTTGG : GTTTGAGCAATATGTAG T A L G C F L S S L M T L L : G L S N M - P R W D A S F R L - - L F W : V - A I C R N R A G M L P F V F D D S F G : F E Q Y V . . . . . . 80021 ATTATGCACTTGATGTCCCAATGTATTTTGTCTATCGGAAGAAGAAGTATGTTGATTGTA I M H L M S Q C I L S I G R R S M L I V L C T - C P N V F C L S E E E V C - L Y D Y A L D V P M Y F V Y R K K K Y V D C . . : . . . . 79961 CTGGATTGTCTTTCCGG : GACTTCATGAATGGAAAACTTCCGCCCATTCCCGGCGAATACC L D C L S G : T S - M E N F R P F P A N T W I V F P : G L H E W K T S A H S R R I P T G L S F R : D F M N G K L P P I P G E Y . . . . . : . 79830 CTACTCTTAATGATTGGGAGAATCATCTCACAACAATATTTCCTGAG : GTCAGACTCAAAA L L L M I G R I I S Q Q Y F L R : S D S K Y S - - L G E S S H N N I S - : G Q T Q K P T L N D W E N H L T T I F P E : V R L K . . . . . . 79681 GATATCTGGAAATGAGAGGTGCTGATGGAGGGCCTTGGAGACGGTTATGCGCATTGCCTG D I W K - E V L M E G L G D G Y A H C L I S G N E R C - W R A L E T V M R I A C R Y L E M R G A D G G P W R R L C A L P . : . . . . . 79621 CATTCTGG : GTGGGTATACTCTACGATGAGGGGTCTTTGCAAAGCGTTTTGGACATGACAT H S G : W V Y S T M R G L C K A F W T - H I L : G G Y T L R - G V F A K R F G H D I A F W : V G I L Y D E G S L Q S V L D M T . . . . 79321 TTGATTGGACTGCAGAAGAAAGAGACATGTTAAGGAAT L I G L Q K K E T C - G - L D C R R K R H V K E F D W T A E E R D M L R N Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-12_AGS-1_PPS_1 (85645 85640,84712 84375,83672 83522,83328 83221,82773 82681,81499 81416,81203 81135,81055 80985,80900 80832,80038 79945,79873 79784,79694 79614,79373 79284) (frame '0'; 1344 bp, 448 residues) 1 AKAQSMALMS QAGSSHCIYS EKVRCISGHR SIINNMDMFR MREICFGVDI SSRNASRRVQ 61 GNYLNHIGVG SRRGDLTIVA ASPPTEDAVV AAEPLTKEDL VGYLASGCKS KEKWRIGTEH 121 EKFGFEFGTL RPMKYDQIAD LLNGIAERFD WEKVMEGDKI IGLKQGKQSI SLEPGGQFEL 181 SGAPLETLHQ TCAEVNSHLY QVKAVAEEMG IGFLGTGFQP KWGLKDIPIM PKGRYEIMRN 241 YMPKVGSLGL DMMFRTCTVQ VNLDFSSEAD MIRKFRAGLA LQPIATALFA NSPFTEGKPN 301 GYLSKRSHIW TDTDNNRAGM LPFVFDDSFG FEQYVDYALD VPMYFVYRKK KYVDCTGLSF 361 RDFMNGKLPP IPGEYPTLND WENHLTTIFP EVRLKRYLEM RGADGGPWRR LCALPAFWVG 421 ILYDEGSLQS VLDMTFDWTA EERDMLRN AGS-2 (81037 80985,80900 80832,80038 79945,79873 79784,79694 79548) SCR (e 1.000 d 0.998 a 0.996,e 0.949 d 1.000 a 0.982,e 1.000 d 0.939 a 0.993,e 1.000 d 0.999 a 0.991,e 1.000) Exon 1 81037 80985 ( 53 n); score: 1.000 Intron 1 80984 80901 ( 84 n); Pd: 0.998 Pa: 0.996 Exon 2 80900 80832 ( 69 n); score: 0.949 Intron 2 80831 80039 ( 793 n); Pd: 1.000 Pa: 0.982 Exon 3 80038 79945 ( 94 n); score: 1.000 Intron 3 79944 79874 ( 71 n); Pd: 0.939 Pa: 0.993 Exon 4 79873 79784 ( 90 n); score: 1.000 Intron 4 79783 79695 ( 89 n); Pd: 0.999 Pa: 0.991 Exon 5 79694 79548 ( 147 n); score: 1.000 PGS (81037 80985,80900 80832,80038 79945,79873 79784,79694 79548) SGN-E269213+ 3-phase translation of AGS-2 (-strand): . . . . . . : 81037 GCAAATTCACCTTTCACTGAAGGAAAACCTAATGGTTATCTCAGCAAGAGAAG : CCACATT A N S P F T E G K P N G Y L S K R S : H I Q I H L S L K E N L M V I S A R E : A T F K F T F H - R K T - W L S Q Q E K : P H . . . . . . 80893 TGGACCGATACAGATAATAACCGCGCTGGGATGCTTCCTTTCGTCTTTGATGACTCTTTT W T D T D N N R A G M L P F V F D D S F G P I Q I I T A L G C F L S S L M T L L L D R Y R - - P R W D A S F R L - - L F . : . . . . . 80833 GG : GTTTGAGCAATATGTAGATTATGCACTTGATGTCCCAATGTATTTTGTCTATCGGAAG G : F E Q Y V D Y A L D V P M Y F V Y R K : G L S N M - I M H L M S Q C I L S I G R W : V - A I C R L C T - C P N V F C L S E . . . . : . . 79980 AAGAAGTATGTTGATTGTACTGGATTGTCTTTCCGG : GACTTCATGAATGGAAAACTTCCG K K Y V D C T G L S F R : D F M N G K L P R S M L I V L D C L S G : T S - M E N F R E E V C - L Y W I V F P : G L H E W K T S . . . . . . 79849 CCCATTCCCGGCGAATACCCTACTCTTAATGATTGGGAGAATCATCTCACAACAATATTT P I P G E Y P T L N D W E N H L T T I F P F P A N T L L L M I G R I I S Q Q Y F A H S R R I P Y S - - L G E S S H N N I . : . . . . . 79789 CCTGAG : GTCAGACTCAAAAGATATCTGGAAATGAGAGGTGCTGATGGAGGGCCTTGGAGA P E : V R L K R Y L E M R G A D G G P W R L R : S D S K D I W K - E V L M E G L G D S - : G Q T Q K I S G N E R C - W R A L E . . . . . . 79640 CGGTTATGCGCATTGCCTGCATTCTGGGTATGCAATCTGACTCTAGGATTTATGATGTTG R L C A L P A F W V C N L T L G F M M L G Y A H C L H S G Y A I - L - D L - C C T V M R I A C I L G M Q S D S R I Y D V . . . . 79580 TTTTTTGTAAAAGAAGGAAACAAAAAGCGAGTA F F V K E G N K K R V F L - K K E T K S E V F C K R R K Q K A S Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-12_AGS-2_PPS_1 (81037 80985,80900 80832,80038 79945,79873 79784,79694 79548) (frame '1'; 453 bp, 151 residues) 1 ANSPFTEGKP NGYLSKRSHI WTDTDNNRAG MLPFVFDDSF GFEQYVDYAL DVPMYFVYRK 61 KKYVDCTGLS FRDFMNGKLP PIPGEYPTLN DWENHLTTIF PEVRLKRYLE MRGADGGPWR 121 RLCALPAFWV CNLTLGFMML FFVKEGNKKR V PGL 13 (- strand): 97107 95797 AGS-1 (97107 95797) SCR (e 0.990) Exon 1 97107 95797 (1311 n); score: 0.990 PGS (96280 95797) SGN-E311217- PGS (96077 95797) SGN-E309792- PGS (96681 95992) SGN-E332140+ PGS (97106 96565) SGN-E206842+ PGS (97107 96578) SGN-E264431+ PGS (97089 96589) SGN-E270165+ PGS (97105 96618) SGN-E206662+ 3-phase translation of AGS-1 (-strand): . . . . . . 97107 GATGGTTGAATCAATTGGTGCAATATCACTTTTAGGGAGGGTGCCAGTTTATGGAGTTAA D G - I N W C N I T F R E G A S L W S - M V E S I G A I S L L G R V P V Y G V N W L N Q L V Q Y H F - G G C Q F M E L . . . . . . 97047 TTGTAGCAAAGATGTGTCGAGTAAACGGAAGTCGATTGATGTATGTCGATTGCCTTCTGC L - Q R C V E - T E V D - C M S I A F C C S K D V S S K R K S I D V C R L P S A I V A K M C R V N G S R L M Y V D C L L . . . . . . 96987 TGATTTTCTTGGCAGAAAGCTTGTTTGTGGTTCGAATTTACTGAGATTAAGAGGTGATTG - F S W Q K A C L W F E F T E I K R - L D F L G R K L V C G S N L L R L R G D C L I F L A E S L F V V R I Y - D - E V I . . . . . . 96927 TGTGAGGTTTTCGTTGATTAGAGGTCAGGCGATGGAATTGACCAAAGAGGCATTTTCGTT C E V F V D - R S G D G I D Q R G I F V V R F S L I R G Q A M E L T K E A F S F V - G F R - L E V R R W N - P K R H F R . . . . . . 96867 CAGAGAGGACGAGAAAATATCTAAAGATTTTCGTTATAGGATTGAGACTGATGTGGATAA Q R G R E N I - R F S L - D - D - C G - R E D E K I S K D F R Y R I E T D V D K S E R T R K Y L K I F V I G L R L M W I . . . . . . 96807 AAAGCCAGGGAAATGGCCACCAACGAATAAAGCTGATAATCCAGCTCTTCATAATCCATT K A R E M A T N E - S - - S S S S - S I K P G K W P P T N K A D N P A L H N P L K S Q G N G H Q R I K L I I Q L F I I H . . . . . . 96747 GCTTAGACAAGAGAGGATGGGCTGTGGATGGTTAGCAGCTATATTTGAATGGGAAGGTGT A - T R E D G L W M V S S Y I - M G R C L R Q E R M G C G W L A A I F E W E G V C L D K R G W A V D G - Q L Y L N G K V . . . . . . 96687 GTTAACTGAAGACAATCCAGATCTCGAGAAGCAGGCGTGGTTAGCTCTGTCTCAGGAAGA V N - R Q S R S R E A G V V S S V S G R L T E D N P D L E K Q A W L A L S Q E E C - L K T I Q I S R S R R G - L C L R K . . . . . . 96627 AGGAAAATCACCTCCTCCGGGTTTTATTTTGAAGAGAATTGAAGGAATGAAGAATGAGCA R K I T S S G F Y F E E N - R N E E - A G K S P P P G F I L K R I E G M K N E Q K E N H L L R V L F - R E L K E - R M S . . . . . . 96567 AGCGATATCAGAAGTTCTATGCTGGTCAAGAGATCCTGTTCAAGTGAAGAGAATGGCTAC S D I R S S M L V K R S C S S E E N G Y A I S E V L C W S R D P V Q V K R M A T K R Y Q K F Y A G Q E I L F K - R E W L . . . . . . 96507 TCGGAAGGAAGATATTTATCAAGCCCTGCAGGGGGGAATATATAGCTTCCGACCTGGATC S E G R Y L S S P A G G N I - L P T W I R K E D I Y Q A L Q G G I Y S F R P G S L G R K I F I K P C R G E Y I A S D L D . . . . . . 96447 ACAGGAGTTCGTCAACACCCTGATGCACTACAAAGTGCCAATGGCTTTAGTGTCTACACG T G V R Q H P D A L Q S A N G F S V Y T Q E F V N T L M H Y K V P M A L V S T R H R S S S T P - C T T K C Q W L - C L H . . . . . . 96387 TCCAAGAAAATATGTCGAGAATGCAATTGGTACCATTGGCTTTGAAGGGATATTTAGTGT S K K I C R E C N W Y H W L - R D I - C P R K Y V E N A I G T I G F E G I F S V V Q E N M S R M Q L V P L A L K G Y L V . . . . . . 96327 AATTGTAGCTGCAGAGGATGTTCACAGAGGGAAGCCTGATCCGGAGATGTTTGTCTATGC N C S C R G C S Q R E A - S G D V C L C I V A A E D V H R G K P D P E M F V Y A - L - L Q R M F T E G S L I R R C L S M . . . . . . 96267 ATCACAGCTTCTACAATTCATACCAGAGAGGTGTATTGTGTTTGGGAACTCAAACCAAAC I T A S T I H T R E V Y C V W E L K P N S Q L L Q F I P E R C I V F G N S N Q T H H S F Y N S Y Q R G V L C L G T Q T K . . . . . . 96207 CGTCGAGGCTGCACATGATGCTCAGATGAAGTGTGTTGCAGTTGCTAGCAAACATCCAGT R R G C T - C S D E V C C S C - Q T S S V E A A H D A Q M K C V A V A S K H P V P S R L H M M L R - S V L Q L L A N I Q . . . . . . 96147 GTATGAGCTTGGTGCTGCTGACTTGGTTGTGAGGCACCTTGATGAGCTCTCGATCGTTGA V - A W C C - L G C E A P - - A L D R - Y E L G A A D L V V R H L D E L S I V D C M S L V L L T W L - G T L M S S R S L . . . . . . 96087 CCTAAAGAACCTTGCTGCTGTTGAGCTGACTGAATTTGGATCTCCTGAACCAGAATTGGA P K E P C C C - A D - I W I S - T R I G L K N L A A V E L T E F G S P E P E L E T - R T L L L L S - L N L D L L N Q N W . . . . . . 96027 AATGGAAGAAGAGGATGATCCTTATCCTCCATCTGCAGTGGCCGTCGATGATGGTTTCTG N G R R G - S L S S I C S G R R - W F L M E E E D D P Y P P S A V A V D D G F W K W K K R M I L I L H L Q W P S M M V S . . . . . . 95967 GTAACATCTCCCCAATGTACAGTAGTCGCGCTTAATAATATTCAAATAGGATATAGAAAA V T S P Q C T V V A L N N I Q I G Y R K - H L P N V Q - S R L I I F K - D I E K G N I S P M Y S S R A - - Y S N R I - K . . . . . . 95907 ATGTAAACTAAAATGCAGGGCAAGAAGTGTATCAGAAGGTGGTCTACATTGGGCTGCCAT M - T K M Q G K K C I R R W S T L G C H C K L K C R A R S V S E G G L H W A A I N V N - N A G Q E V Y Q K V V Y I G L P . . . . . . 95847 CAACCTGACTTTGTATGTGATATTTGTATTATATATGTATATCTTCTGACC Q P D F V C D I C I I Y V Y L L T N L T L Y V I F V L Y M Y I F - S T - L C M - Y L Y Y I C I S S D Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-13_AGS-1_PPS_1 (97106 95964) (frame '2'; 1140 bp, 380 residues) 1 MVESIGAISL LGRVPVYGVN CSKDVSSKRK SIDVCRLPSA DFLGRKLVCG SNLLRLRGDC 61 VRFSLIRGQA MELTKEAFSF REDEKISKDF RYRIETDVDK KPGKWPPTNK ADNPALHNPL 121 LRQERMGCGW LAAIFEWEGV LTEDNPDLEK QAWLALSQEE GKSPPPGFIL KRIEGMKNEQ 181 AISEVLCWSR DPVQVKRMAT RKEDIYQALQ GGIYSFRPGS QEFVNTLMHY KVPMALVSTR 241 PRKYVENAIG TIGFEGIFSV IVAAEDVHRG KPDPEMFVYA SQLLQFIPER CIVFGNSNQT 301 VEAAHDAQMK CVAVASKHPV YELGAADLVV RHLDELSIVD LKNLAAVELT EFGSPEPELE 361 MEEEDDPYPP SAVAVDDGFW - 3-phase translation of AGS-1 (+strand): . . . . . . 95797 GGTCAGAAGATATACATATATAATACAAATATCACATACAAAGTCAGGTTGATGGCAGCC G Q K I Y I Y N T N I T Y K V R L M A A V R R Y T Y I I Q I S H T K S G - W Q P S E D I H I - Y K Y H I Q S Q V D G S . . . . . . 95857 CAATGTAGACCACCTTCTGATACACTTCTTGCCCTGCATTTTAGTTTACATTTTTCTATA Q C R P P S D T L L A L H F S L H F S I N V D H L L I H F L P C I L V Y I F L Y P M - T T F - Y T S C P A F - F T F F Y . . . . . . 95917 TCCTATTTGAATATTATTAAGCGCGACTACTGTACATTGGGGAGATGTTACCAGAAACCA S Y L N I I K R D Y C T L G R C Y Q K P P I - I L L S A T T V H W G D V T R N H I L F E Y Y - A R L L Y I G E M L P E T . . . . . . 95977 TCATCGACGGCCACTGCAGATGGAGGATAAGGATCATCCTCTTCTTCCATTTCCAATTCT S S T A T A D G G - G S S S S S I S N S H R R P L Q M E D K D H P L L P F P I L I I D G H C R W R I R I I L F F H F Q F . . . . . . 96037 GGTTCAGGAGATCCAAATTCAGTCAGCTCAACAGCAGCAAGGTTCTTTAGGTCAACGATC G S G D P N S V S S T A A R F F R S T I V Q E I Q I Q S A Q Q Q Q G S L G Q R S W F R R S K F S Q L N S S K V L - V N D . . . . . . 96097 GAGAGCTCATCAAGGTGCCTCACAACCAAGTCAGCAGCACCAAGCTCATACACTGGATGT E S S S R C L T T K S A A P S S Y T G C R A H Q G A S Q P S Q Q H Q A H T L D V R E L I K V P H N Q V S S T K L I H W M . . . . . . 96157 TTGCTAGCAACTGCAACACACTTCATCTGAGCATCATGTGCAGCCTCGACGGTTTGGTTT L L A T A T H F I - A S C A A S T V W F C - Q L Q H T S S E H H V Q P R R F G L F A S N C N T L H L S I M C S L D G L V . . . . . . 96217 GAGTTCCCAAACACAATACACCTCTCTGGTATGAATTGTAGAAGCTGTGATGCATAGACA E F P N T I H L S G M N C R S C D A - T S S Q T Q Y T S L V - I V E A V M H R Q - V P K H N T P L W Y E L - K L - C I D . . . . . . 96277 AACATCTCCGGATCAGGCTTCCCTCTGTGAACATCCTCTGCAGCTACAATTACACTAAAT N I S G S G F P L - T S S A A T I T L N T S P D Q A S L C E H P L Q L Q L H - I K H L R I R L P S V N I L C S Y N Y T K . . . . . . 96337 ATCCCTTCAAAGCCAATGGTACCAATTGCATTCTCGACATATTTTCTTGGACGTGTAGAC I P S K P M V P I A F S T Y F L G R V D S L Q S Q W Y Q L H S R H I F L D V - T Y P F K A N G T N C I L D I F S W T C R . . . . . . 96397 ACTAAAGCCATTGGCACTTTGTAGTGCATCAGGGTGTTGACGAACTCCTGTGATCCAGGT T K A I G T L - C I R V L T N S C D P G L K P L A L C S A S G C - R T P V I Q V H - S H W H F V V H Q G V D E L L - S R . . . . . . 96457 CGGAAGCTATATATTCCCCCCTGCAGGGCTTGATAAATATCTTCCTTCCGAGTAGCCATT R K L Y I P P C R A - - I S S F R V A I G S Y I F P P A G L D K Y L P S E - P F S E A I Y S P L Q G L I N I F L P S S H . . . . . . 96517 CTCTTCACTTGAACAGGATCTCTTGACCAGCATAGAACTTCTGATATCGCTTGCTCATTC L F T - T G S L D Q H R T S D I A C S F S S L E Q D L L T S I E L L I S L A H S S L H L N R I S - P A - N F - Y R L L I . . . . . . 96577 TTCATTCCTTCAATTCTCTTCAAAATAAAACCCGGAGGAGGTGATTTTCCTTCTTCCTGA F I P S I L F K I K P G G G D F P S S - S F L Q F S S K - N P E E V I F L L P E L H S F N S L Q N K T R R R - F S F F L . . . . . . 96637 GACAGAGCTAACCACGCCTGCTTCTCGAGATCTGGATTGTCTTCAGTTAACACACCTTCC D R A N H A C F S R S G L S S V N T P S T E L T T P A S R D L D C L Q L T H L P R Q S - P R L L L E I W I V F S - H T F . . . . . . 96697 CATTCAAATATAGCTGCTAACCATCCACAGCCCATCCTCTCTTGTCTAAGCAATGGATTA H S N I A A N H P Q P I L S C L S N G L I Q I - L L T I H S P S S L V - A M D Y P F K Y S C - P S T A H P L L S K Q W I . . . . . . 96757 TGAAGAGCTGGATTATCAGCTTTATTCGTTGGTGGCCATTTCCCTGGCTTTTTATCCACA - R A G L S A L F V G G H F P G F L S T E E L D Y Q L Y S L V A I S L A F Y P H M K S W I I S F I R W W P F P W L F I H . . . . . . 96817 TCAGTCTCAATCCTATAACGAAAATCTTTAGATATTTTCTCGTCCTCTCTGAACGAAAAT S V S I L - R K S L D I F S S S L N E N Q S Q S Y N E N L - I F S R P L - T K M I S L N P I T K I F R Y F L V L S E R K . . . . . . 96877 GCCTCTTTGGTCAATTCCATCGCCTGACCTCTAATCAACGAAAACCTCACACAATCACCT A S L V N S I A - P L I N E N L T Q S P P L W S I P S P D L - S T K T S H N H L C L F G Q F H R L T S N Q R K P H T I T . . . . . . 96937 CTTAATCTCAGTAAATTCGAACCACAAACAAGCTTTCTGCCAAGAAAATCAGCAGAAGGC L N L S K F E P Q T S F L P R K S A E G L I S V N S N H K Q A F C Q E N Q Q K A S - S Q - I R T T N K L S A K K I S R R . . . . . . 96997 AATCGACATACATCAATCGACTTCCGTTTACTCGACACATCTTTGCTACAATTAACTCCA N R H T S I D F R L L D T S L L Q L T P I D I H Q S T S V Y S T H L C Y N - L H Q S T Y I N R L P F T R H I F A T I N S . . . . . . 97057 TAAACTGGCACCCTCCCTAAAAGTGATATTGCACCAATTGATTCAACCATC - T G T L P K S D I A P I D S T I K L A P S L K V I L H Q L I Q P I N W H P P - K - Y C T N - F N H Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-13_AGS-1_PPS_1 (95927 96163) (frame '2'; 234 bp, 78 residues) 1 ILLSATTVHW GDVTRNHHRR PLQMEDKDHP LLPFPILVQE IQIQSAQQQQ GSLGQRSRAH 61 QGASQPSQQH QAHTLDVC- >C08HBa0251H04.1+_PGL-13_AGS-1_PPS_2 (96717 96941) (frame '0'; 222 bp, 74 residues) 1 PSTAHPLLSK QWIMKSWIIS FIRWWPFPWL FIHISLNPIT KIFRYFLVLS ERKCLFGQFH 61 RLTSNQRKPH TITS- >C08HBa0251H04.1+_PGL-13_AGS-1_PPS_3 (95797 96006) (frame '1'; 207 bp, 69 residues) 1 GQKIYIYNTN ITYKVRLMAA QCRPPSDTLL ALHFSLHFSI SYLNIIKRDY CTLGRCYQKP 61 SSTATADGG- PGL 14 (+ strand): 100444 101710 AGS-1 (100444 100761,101560 101710) SCR (e 1.000 d 0.899 a 0.999,e 0.987) Exon 1 100444 100761 ( 318 n); score: 1.000 Intron 1 100762 101559 ( 798 n); Pd: 0.899 Pa: 0.999 Exon 2 101560 101710 ( 151 n); score: 0.987 PGS (100444 100761,101560 101710) SGN-E315454+ 3-phase translation of AGS-1 (+strand): . . . . . . 100444 GGCGGCTCGCTTTACCGACTCCGGCGACGGCGGAAAACCTTATCCGACGGCAAAAATGGG G G S L Y R L R R R R K T L S D G K N G A A R F T D S G D G G K P Y P T A K M G R L A L P T P A T A E N L I R R Q K W . . . . . . 100504 TAAGAAACCGTCGAGGAAGAATGGAGTTGGTAAAGCATCTGGTTCAATTACATTGCGAGA - E T V E E E W S W - S I W F N Y I A R K K P S R K N G V G K A S G S I T L R E V R N R R G R M E L V K H L V Q L H C E . . . . . . 100564 AGAATCAGGTGTGAAGAAGAAACAAACTCTTGTTAATGCGAAATCGATGCTGAAATTGGA R I R C E E E T N S C - C E I D A E I G E S G V K K K Q T L V N A K S M L K L E K N Q V - R R N K L L L M R N R C - N W . . . . . . 100624 GCATATAAAGGATATTGCTAACTGGACTAGTGGAGAAGGTTCCATACCTTCACTTGCTGC A Y K G Y C - L D - W R R F H T F T C C H I K D I A N W T S G E G S I P S L A A S I - R I L L T G L V E K V P Y L H L L . . . . . . 100684 GTTTTTTGGGCAGAGATTAGCTGTATCAGCTGAGTCTTTGGGTGTTTCACCTGACCCTTC V F W A E I S C I S - V F G C F T - P F F F G Q R L A V S A E S L G V S P D P S R F L G R D - L Y Q L S L W V F H L T L . . : . . . . 100744 ATTATTAACCTGTCAAAG : GTGTGAATCTATCCTTCAGGCTGGCTACAATAGTACAGCACG I I N L S K : V - I Y P S G W L Q - Y S T L L T C Q R : C E S I L Q A G Y N S T A R H Y - P V K : G V N L S F R L A T I V Q H . . . . . . 101602 GATTGAGAAGAACAAAAGAAAGGCACGAAAGAAACGCAAAACATCAGGTATTCCCTCGAA D - E E Q K K G T K E T Q N I R Y S L E I E K N K R K A R K K R K T S G I P S K G L R R T K E R H E R N A K H Q V F P R . . . . . 101662 GAACTCAGTTGTATATGAGTGTCATTTCTGCTCACATCGAAATCTAAAG E L S C I - V S F L L T S K S K N S V V Y E C H F C S H R N L K R T Q L Y M S V I S A H I E I - Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-14_AGS-1_PPS_1 (100445 100761,101560 101710) (frame '2'; 468 bp, 156 residues) 1 AARFTDSGDG GKPYPTAKMG KKPSRKNGVG KASGSITLRE ESGVKKKQTL VNAKSMLKLE 61 HIKDIANWTS GEGSIPSLAA FFGQRLAVSA ESLGVSPDPS LLTCQRCESI LQAGYNSTAR 121 IEKNKRKARK KRKTSGIPSK NSVVYECHFC SHRNLK PGL 15 (- strand): 126844 122871 AGS-1 (126844 126378,126256 126138,125879 125755,123850 123751,123589 123512,123266 123183,122910 122871) SCR (e 1.000 d 0.937 a 0.946,e 1.000 d 0.988 a 0.991,e 1.000 d 0.997 a 0.763,e 1.000 d 0.998 a 0.932,e 1.000 d 0.976 a 0.822,e 1.000 d 0.998 a 0.995,e 1.000) Exon 1 126844 126378 ( 467 n); score: 1.000 Intron 1 126377 126257 ( 121 n); Pd: 0.937 Pa: 0.946 Exon 2 126256 126138 ( 119 n); score: 1.000 Intron 2 126137 125880 ( 258 n); Pd: 0.988 Pa: 0.991 Exon 3 125879 125755 ( 125 n); score: 1.000 Intron 3 125754 123851 (1904 n); Pd: 0.997 Pa: 0.763 Exon 4 123850 123751 ( 100 n); score: 1.000 Intron 4 123750 123590 ( 161 n); Pd: 0.998 Pa: 0.932 Exon 5 123589 123512 ( 78 n); score: 1.000 Intron 5 123511 123267 ( 245 n); Pd: 0.976 Pa: 0.822 Exon 6 123266 123183 ( 84 n); score: 1.000 Intron 6 123182 122911 ( 272 n); Pd: 0.998 Pa: 0.995 Exon 7 122910 122871 ( 40 n); score: 1.000 PGS (126467 126378,126256 126138,125879 125755,123850 123751,123589 123512,123266 123183,122910 122871) SGN-E268933+ PGS (126844 126378,126256 126138,125879 125816) SGN-E342027+ 3-phase translation of AGS-1 (-strand): . . . . . . 126844 TGGTTCATTGTTAAGTCAGATGGCAAAGCATGATGAGATCGACAGGGTGTCAACAGGCAC W F I V K S D G K A - - D R Q G V N R H G S L L S Q M A K H D E I D R V S T G T V H C - V R W Q S M M R S T G C Q Q A . . . . . . 126784 TTCATCTATAAGGCACTATAATGAAGTTTTTCATGACAAAAAGTCAAACACACTAGGGAT F I Y K A L - - S F S - Q K V K H T R D S S I R H Y N E V F H D K K S N T L G I L H L - G T I M K F F M T K S Q T H - G . . . . . . 126724 TTCACATTCCGAAAATAACTTAGTCTTCCATGAGTACTCGGTCACTCCAAGTTGTTCTGC F T F R K - L S L P - V L G H S K L F C S H S E N N L V F H E Y S V T P S C S A F H I P K I T - S S M S T R S L Q V V L . . . . . . 126664 TAGTGTACATGACAAAATTAATGCTAAGGATCACATATATGATTCTGCCATTAATGAAAA - C T - Q N - C - G S H I - F C H - - K S V H D K I N A K D H I Y D S A I N E K L V Y M T K L M L R I T Y M I L P L M K . . . . . . 126604 AGTAGAACTGGAGGAAGAGCTCAGTTCTGAAGTACAGAGATCTGTGGCAAAGGAAAAGCC S R T G G R A Q F - S T E I C G K G K A V E L E E E L S S E V Q R S V A K E K P K - N W R K S S V L K Y R D L W Q R K S . . . . . . 126544 TCTGTATGCACTTGCAAAACAAGAGCATGCTTTTGCAGGAGCAATGGCTGGAATTTTTGT S V C T C K T R A C F C R S N G W N F C L Y A L A K Q E H A F A G A M A G I F V L C M H L Q N K S M L L Q E Q W L E F L . . . . . . 126484 CAGCCTTTGTCTTCATCCAGTTGACACAATTAAGACTGTTATTCAGTCATGTCCCAATGA Q P L S S S S - H N - D C Y S V M S Q - S L C L H P V D T I K T V I Q S C P N D S A F V F I Q L T Q L R L L F S H V P M . . . . . : . 126424 TCAGAAGCCACTTTATTATATCGGCAGATCATTCATTTCTGAAAGAG : GAGTAACAGCACT S E A T L L Y R Q I I H F - K R : S N S T Q K P L Y Y I G R S F I S E R : G V T A L I R S H F I I S A D H S F L K E : E - Q H . . . . . . 126243 TTATCGGGGAATTTCGACCAATCTTGCTTCGTCTGCACCCATATCTGCACTTTACACCTT L S G N F D Q S C F V C T H I C T L H L Y R G I S T N L A S S A P I S A L Y T F F I G E F R P I L L R L H P Y L H F T P . . . . . : . 126183 TACATACGAATCAGTTAAAGGGGCCTTACTACCTCTTTTTCCTAAG : GAATGCCACTCTTT Y I R I S - R G L T T S F S - : G M P L F T Y E S V K G A L L P L F P K : E C H S F L H T N Q L K G P Y Y L F F L R : N A T L . . . . . . 125865 TGCACACTGCTTGGCTGGGGGTTCTGCAAGCATTGCCACTTCATTCATTTTCACTCCGAG C T L L G W G F C K H C H F I H F H S E A H C L A G G S A S I A T S F I F T P S L H T A W L G V L Q A L P L H S F S L R . . . . . . : 125805 TGAGCGCATTAAACAACAGATGCAAGTGGGCTCCCACTATAAGAACTGCTG : GAGTGCCTT - A H - T T D A S G L P L - E L L : E C L E R I K Q Q M Q V G S H Y K N C W : S A L V S A L N N R C K W A P T I R T A : G V P . . . . . . 123841 AATTGTGATTACAAGAAGTGGTGGTTTGCCTTCTCTGTATGCTGGATGGAAAGCTGTACT N C D Y K K W W F A F S V C W M E S C T I V I T R S G G L P S L Y A G W K A V L - L - L Q E V V V C L L C M L D G K L Y . . . . : . . 123781 CTGGAGGAATATTCCACACTCAATCATTAAG : TTCTATACATATGAAAGATTGAAGGAGTT L E E Y S T L N H - : V L Y I - K I E G V W R N I P H S I I K : F Y T Y E R L K E L S G G I F H T Q S L S : S I H M K D - R S . . . . . : . 123560 GAGGTTATCATCAGTTCAACTACGCAACCAAAATGACACACTAATGACA : CTTGCCTGTGG E V I I S S T T Q P K - H T N D : T C L W R L S S V Q L R N Q N D T L M T : L A C G - G Y H Q F N Y A T K M T H - - H : L P V . . . . . . 123255 AGGATTAGCTGGCTCTACTGCTGCACTATTCACAACTCCCTTTGATGTTGTCAAGACAAG R I S W L Y C C T I H N S L - C C Q D K G L A G S T A A L F T T P F D V V K T R E D - L A L L L H Y S Q L P L M L S R Q . . : . . . . 123195 ATTACAGACACAG : ATTCCTGGATCTATGACTCAGTTTGGTGTATTCGGCACAC I T D T : D S W I Y D S V W C I R H L Q T Q : I P G S M T Q F G V F G T D Y R H R : F L D L - L S L V Y S A H Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-15_AGS-1_PPS_1 (126843 126378,126256 126138,125879 125755,123850 123751,123589 123512,123266 123183,122910 122872) (frame '2'; 1011 bp, 337 residues) 1 GSLLSQMAKH DEIDRVSTGT SSIRHYNEVF HDKKSNTLGI SHSENNLVFH EYSVTPSCSA 61 SVHDKINAKD HIYDSAINEK VELEEELSSE VQRSVAKEKP LYALAKQEHA FAGAMAGIFV 121 SLCLHPVDTI KTVIQSCPND QKPLYYIGRS FISERGVTAL YRGISTNLAS SAPISALYTF 181 TYESVKGALL PLFPKECHSF AHCLAGGSAS IATSFIFTPS ERIKQQMQVG SHYKNCWSAL 241 IVITRSGGLP SLYAGWKAVL WRNIPHSIIK FYTYERLKEL RLSSVQLRNQ NDTLMTLACG 301 GLAGSTAALF TTPFDVVKTR LQTQIPGSMT QFGVFGT PGL 16 (- strand): 129889 127171 AGS-1 (129889 129675,128386 128306,127640 127171) SCR (e 1.000 d 0.865 a 0.576,e 1.000 d 0.999 a 0.000,e 0.995) Exon 1 129889 129675 ( 215 n); score: 1.000 Intron 1 129674 128387 (1288 n); Pd: 0.865 Pa: 0.576 Exon 2 128386 128306 ( 81 n); score: 1.000 Intron 2 128305 127641 ( 665 n); Pd: 0.999 Pa: 0.000 Exon 3 127640 127171 ( 470 n); score: 0.995 PGS (129889 129675,128386 128306,127640 127171) SGN-E318360+ 3-phase translation of AGS-1 (-strand): . . . . . . 129889 CACAACTCATTGTTTTACCAATTATAGGACTATGGAAGCTGAAACATCCCAATTCTGAAT H N S L F Y Q L - D Y G S - N I P I L N T T H C F T N Y R T M E A E T S Q F - I Q L I V L P I I G L W K L K H P N S E . . . . . . 129829 TTGGTATTTTTTTTCTTCATTAACCCTACTAACTTGGGGCTGTCATGGGGGAAATTTGCA L V F F F F I N P T N L G L S W G K F A W Y F F S S L T L L T W G C H G G N L H F G I F F L H - P Y - L G A V M G E I C . . . . . . 129769 TCCAATTTCTCTCTGATTTGCCCCAGGTGAAAGATGATTCACAGCATTCTTGGCACATTG S N F S L I C P R - K M I H S I L G T L P I S L - F A P G E R - F T A F L A H C I Q F L S D L P Q V K D D S Q H S W H I . . . . : . . 129709 TTTTCAGCTGCTACAAAACAGTCAATTTCATTCAG : GTGTAATTTGTTACTACTTGATTTG F S A A T K Q S I S F R : C N L L L L D L F Q L L Q N S Q F H S : G V I C Y Y L I C V F S C Y K T V N F I Q : V - F V T T - F . . . . . . : 128361 TTGTGTCCAATGGAACTAGTGGCTTGGGACTAGTAAGAGGAAGGAAGCTTTGACAG : TTGG L C P M E L V A W D - - E E G S F D S : W C V Q W N - W L G T S K R K E A L T : V G V V S N G T S G L G L V R G R K L - Q : L . . . . . . 127636 CAATCTTCTAGCATTTTATATTTTGGAGAACATTTTAAATCATTGTGATAATATGGCTGG Q S S S I L Y F G E H F K S L - - Y G W N L L A F Y I L E N I L N H C D N M A G A I F - H F I F W R T F - I I V I I W L . . . . . . 127576 GGGAAGTCAACCACGCCAAAGTGACAAACCTTCAATCAGATACAGGTGTAATCTACTTGA G K S T T P K - Q T F N Q I Q V - S T - G S Q P R Q S D K P S I R Y R C N L L D G E V N H A K V T N L Q S D T G V I Y L . . . . . . 127516 TGGGGCACTGTTTGAAATCACTGATTTAGACCGACAGAGTAATGCCCCTATTTCTTCTGG W G T V - N H - F R P T E - C P Y F F W G A L F E I T D L D R Q S N A P I S S G M G H C L K S L I - T D R V M P L F L L . . . . . . 127456 AAATAAGGAGAACCCTAAAAATTCTGACTCTGAATCTTCTAAGATAATGAGCACAAGTGA K - G E P - K F - L - I F - D N E H K - N K E N P K N S D S E S S K I M S T S E E I R R T L K I L T L N L L R - - A Q V . . . . . . 127396 GCTTATATCAGCAGTTGGAAGTATATGGTGTTCTGCAGCTGGCCCTGTTACACGAATCCT A Y I S S W K Y M V F C S W P C Y T N P L I S A V G S I W C S A A G P V T R I L S L Y Q Q L E V Y G V L Q L A L L H E S . . . . . . 127336 ATCTAAAGGAAGTTCTAGATGCAATAATACCGATCACCAAGAAAACAACATATTTGGTTA I - R K F - M Q - Y R S P R K Q H I W L S K G S S R C N N T D H Q E N N I F G Y Y L K E V L D A I I P I T K K T T Y L V . . . . . . 127276 CTCCACTGCAGATAAAATTTCTGGTGTTTGTTTTTCACCTCCTGATCACTCAATACCAGT L H C R - N F W C L F F T S - S L N T S S T A D K I S G V C F S P P D H S I P V T P L Q I K F L V F V F H L L I T Q Y Q . . . . . 127216 CAATTTGGATAGTGATACAGATTCTTCACCTCTACTGCTTGCAAAT Q F G - - Y R F F T S T A C K N L D S D T D S S P L L L A N S I W I V I Q I L H L Y C L Q Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-16_AGS-1_PPS_1 (128342 128306,127640 127171) (frame '2'; 507 bp, 169 residues) 1 WLGTSKRKEA LTVGNLLAFY ILENILNHCD NMAGGSQPRQ SDKPSIRYRC NLLDGALFEI 61 TDLDRQSNAP ISSGNKENPK NSDSESSKIM STSELISAVG SIWCSAAGPV TRILSKGSSR 121 CNNTDHQENN IFGYSTADKI SGVCFSPPDH SIPVNLDSDT DSSPLLLAN PGL 17 (- strand): 134896 131971 AGS-1 (134896 134816,134592 134282,133705 133618,133466 133381,132642 132564,132462 132403,132011 131971) SCR (e 1.000 d 0.655 a 0.777,e 1.000 d 0.996 a 0.987,e 1.000 d 0.987 a 0.991,e 1.000 d 0.895 a 0.147,e 1.000 d 0.999 a 1.000,e 0.983 d 0.999 a 0.611,e 0.976) Exon 1 134896 134816 ( 81 n); score: 1.000 Intron 1 134815 134593 ( 223 n); Pd: 0.655 Pa: 0.777 Exon 2 134592 134282 ( 311 n); score: 1.000 Intron 2 134281 133706 ( 576 n); Pd: 0.996 Pa: 0.987 Exon 3 133705 133618 ( 88 n); score: 1.000 Intron 3 133617 133467 ( 151 n); Pd: 0.987 Pa: 0.991 Exon 4 133466 133381 ( 86 n); score: 1.000 Intron 4 133380 132643 ( 738 n); Pd: 0.895 Pa: 0.147 Exon 5 132642 132564 ( 79 n); score: 1.000 Intron 5 132563 132463 ( 101 n); Pd: 0.999 Pa: 1.000 Exon 6 132462 132403 ( 60 n); score: 0.983 Intron 6 132402 132012 ( 391 n); Pd: 0.999 Pa: 0.611 Exon 7 132011 131971 ( 41 n); score: 0.976 PGS (134896 134816,134592 134282,133705 133618,133466 133381,132642 132564,132462 132403,132011 131971) SGN-E327710+ PGS (134872 134816,134592 134282,133705 133618,133466 133381,132642 132588) SGN-E332397+ PGS (134870 134816,134592 134282,133705 133618,133466 133381,132642 132588) SGN-E329166+ PGS (134896 134816,134592 134282,133705 133618,133466 133381,132642 132602) SGN-E328376+ PGS (134824 134816,134592 134282,133705 133618,133466 133381,132642 132606) SGN-E271433+ 3-phase translation of AGS-1 (-strand): . . . . . . 134896 TTTTTCAATCCTCCAAAGTTGCAGTTTTTAGAGAGATTTTAGCTCAACAACTCATGTTAA F F N P P K L Q F L E R F - L N N S C - F S I L Q S C S F - R D F S S T T H V K F Q S S K V A V F R E I L A Q Q L M L . . . : . . . 134836 ACAGTGGTACAACTCAAAAAG : ATGTCTGTTGTAAATCAACAATTTTTCAACCCCATATGG T V V Q L K K : M S V V N Q Q F F N P I W Q W Y N S K R : C L L - I N N F S T P Y G N S G T T Q K : D V C C K S T I F Q P H M . . . . . . 134553 AAGCCATGTGTTTCTTCATGTCCATTGTTAGCTGCACAGTTTGTTGTGATGCCAATGAGT K P C V S S C P L L A A Q F V V M P M S S H V F L H V H C - L H S L L - C Q - V E A M C F F M S I V S C T V C C D A N E . . . . . . 134493 AATAGAGAGCTTTCTGTTGTTTCCACCATTGCTGCTGCAAAATCTACAGCTTCGATTGGT N R E L S V V S T I A A A K S T A S I G I E S F L L F P P L L L Q N L Q L R L V - - R A F C C F H H C C C K I Y S F D W . . . . . . 134433 GAAGGAAATGATGATTATGTTGATATTAGTAGTGGGAAAGCAGCAGCCAAAGGGTCGGGT E G N D D Y V D I S S G K A A A K G S G K E M M I M L I L V V G K Q Q P K G R V - R K - - L C - Y - - W E S S S Q R V G . . . . . . 134373 ACTACGGCGAGAGGAAGGAGGTTGTTGAAGGTTAGGGAGGAGAAGAGGAAACGGGAATAT T T A R G R R L L K V R E E K R K R E Y L R R E E G G C - R L G R R R G N G N M Y Y G E R K E V V E G - G G E E E T G I . . . . : . . 134313 GAACGTCTTCACAATTACCCTGCTTGGGCAAA : AGTGTTGGAAGATGCCTGTAAACATGAT E R L H N Y P A W A K : V L E D A C K H D N V F T I T L L G Q : K C W K M P V N M I - T S S Q L P C L G K : S V G R C L - T - . . . . . . : 133677 ACAGAGCTTCGTGCTGTGCTTGGTGATAGCATTGGAAATCCAGAGCTTATGAGGAAAAAG : T E L R A V L G D S I G N P E L M R K K : Q S F V L C L V I A L E I Q S L - G K R : Y R A S C C A W - - H W K S R A Y E E K : . . . . . . 133466 GTCGAAGAGAGAGTTCGAACAAAGGGCCGAAATTTTCAGAAGTCTAAAACAGGATCAGTT V E E R V R T K G R N F Q K S K T G S V S K R E F E Q R A E I F R S L K Q D Q F G R R E S S N K G P K F S E V - N R I S . . . : . . . 133406 CTTGCATTCAAAGTCAGCTTTAGAGA : TTTTAATCCTCTTGATTCATACATATGGTTTGAA L A F K V S F R D : F N P L D S Y I W F E L H S K S A L E : I L I L L I H T Y G L N S C I Q S Q L - R : F - S S - F I H M V - . . . . . : . 132608 CTCTATGGCTCACCATCTGATCGAGACGTTAACATTTTCGGTAGT : GTTATCCAGGCATGG L Y G S P S D R D V N I F G S : V I Q A W S M A H H L I E T L T F S V V : L S R H G T L W L T I - S R R - H F R - : C Y P G M . . . . . : . 132447 TACGTCATGGGACGTTTAGGTGCATTTAACTCATCGAATTTGCAG : CTGGGGGGTTCATCA Y V M G R L G A F N S S N L Q : L G G S S T S W D V - V H L T H R I C S : W G V H Q V R H G T F R C I - L I E F A : A G G F I . . . 131996 ATGGAATTCAATCCTCTCTATGATGC M E F N P L Y D W N S I L S M M N G I Q S S L - C Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-17_AGS-1_PPS_1 (134836 134816,134592 134282,133705 133618,133466 133381,132642 132564,132462 132403,132011 131973) (frame '1'; 684 bp, 228 residues) 1 TVVQLKKMSV VNQQFFNPIW KPCVSSCPLL AAQFVVMPMS NRELSVVSTI AAAKSTASIG 61 EGNDDYVDIS SGKAAAKGSG TTARGRRLLK VREEKRKREY ERLHNYPAWA KVLEDACKHD 121 TELRAVLGDS IGNPELMRKK VEERVRTKGR NFQKSKTGSV LAFKVSFRDF NPLDSYIWFE 181 LYGSPSDRDV NIFGSVIQAW YVMGRLGAFN SSNLQLGGSS MEFNPLYD PGL 18 (- strand): 143003 139908 AGS-1 (143003 142862,142668 142504,142415 142162,142039 141876,141600 141038,140527 140427,140272 139908) SCR (e 1.000 d 0.990 a 0.000,e 1.000 d 0.951 a 0.997,e 1.000 d 0.869 a 0.998,e 1.000 d 0.997 a 0.998,e 1.000 d 0.999 a 0.999,e 1.000 d 0.999 a 0.996,e 0.997) Exon 1 143003 142862 ( 142 n); score: 1.000 Intron 1 142861 142669 ( 193 n); Pd: 0.990 Pa: 0.000 Exon 2 142668 142504 ( 165 n); score: 1.000 Intron 2 142503 142416 ( 88 n); Pd: 0.951 Pa: 0.997 Exon 3 142415 142162 ( 254 n); score: 1.000 Intron 3 142161 142040 ( 122 n); Pd: 0.869 Pa: 0.998 Exon 4 142039 141876 ( 164 n); score: 1.000 Intron 4 141875 141601 ( 275 n); Pd: 0.997 Pa: 0.998 Exon 5 141600 141038 ( 563 n); score: 1.000 Intron 5 141037 140528 ( 510 n); Pd: 0.999 Pa: 0.999 Exon 6 140527 140427 ( 101 n); score: 1.000 Intron 6 140426 140273 ( 154 n); Pd: 0.999 Pa: 0.996 Exon 7 140272 139908 ( 365 n); score: 0.997 PGS (140265 139908) SGN-E548479- PGS (140265 139908) SGN-E541013+ PGS (140264 139908) SGN-E293454+ PGS (140264 139908) SGN-E293455+ PGS (140264 139908) SGN-E293630+ PGS (141889 141876,141600 141038,140527 140427,140272 140236) SGN-E316968+ PGS (142278 142162,142039 141876,141600 141076) SGN-E262026+ PGS (143003 142862,142668 142504,142415 142162,142039 141876,141600 141572) SGN-E332626+ 3-phase translation of AGS-1 (-strand): . . . . . . 143003 TCAACTACCGAGCTCTGAATGATCTATTCAACATCTCCCAGAGTAGGAAAAGTTCCATTG S T T E L - M I Y S T S P R V G K V P L Q L P S S E - S I Q H L P E - E K F H C N Y R A L N D L F N I S Q S R K S S I . . . . . . 142943 CATATGAAGTTGGTGTTCAAATGGTTGAGATATACAATGAGCAAGTGCGAGACTTGCTCT H M K L V F K W L R Y T M S K C E T C S I - S W C S N G - D I Q - A S A R L A L A Y E V G V Q M V E I Y N E Q V R D L L . . . : . . . 142883 GCAGTGATACTTCTCAGAAACG : ACTTGGGATTTGGAGTACTACTCAACCCAATGGATTAG A V I L L R N : D L G F G V L L N P M D - Q - Y F S E T : T W D L E Y Y S T Q W I S C S D T S Q K R : L G I W S T T Q P N G L . . . . . . 142630 CTGTCCCTGATGCTAGCATGCATCCAGTCAAATCAACTGCAAATGTCTTAGAATTAATGA L S L M L A C I Q S N Q L Q M S - N - - C P - C - H A S S Q I N C K C L R I N E A V P D A S M H P V K S T A N V L E L M . . . . . . 142570 ATATTGGCCTAATGAATAGAGCTGTTGGCGCTACTGCTTTAAATGAAAGAAGTAGTCGGT I L A - - I E L L A L L L - M K E V V G Y W P N E - S C W R Y C F K - K K - S V N I G L M N R A V G A T A L N E R S S R . : . . . . . 142510 CACATAG : TATCCTCACCGTTCACGTGCGCGGTATAGACTTGGAAACAAATGATATTCTAC H I : V S S P F T C A V - T W K Q M I F Y T - : Y P H R S R A R Y R L G N K - Y S T S H S : I L T V H V R G I D L E T N D I L . . . . . . 142362 GAGGCTGCCTACATTTGGTAGATTTGGCTGGCAGTGAAAGAGTAGATCGCTCTGAAGCCA E A A Y I W - I W L A V K E - I A L K P R L P T F G R F G W Q - K S R S L - S H R G C L H L V D L A G S E R V D R S E A . . . . . . 142302 CAGGGGATCGTTTACGAGAAGCACAACATATAAACAAATCATTATCTGCTCTTGGGGATG Q G I V Y E K H N I - T N H Y L L L G M R G S F T R S T T Y K Q I I I C S W G C T G D R L R E A Q H I N K S L S A L G D . . . . . . 142242 TGATATTTGCATTGGCGCAAAAAAGTTCTCATGTGCCATATAGAAATAGCAAACTAACAC - Y L H W R K K V L M C H I E I A N - H D I C I G A K K F S C A I - K - Q T N T V I F A L A Q K S S H V P Y R N S K L T . . . : . . . 142182 AAGTTCTTCAAAGCTCCCTTG : GAGGCCAGGCAAAGACACTTATGTTTGTACAGCTCAATC K F F K A P L : E A R Q R H L C L Y S S I S S S K L P W : R P G K D T Y V C T A Q S Q V L Q S S L : G G Q A K T L M F V Q L N . . . . . . 142000 CTGACGTAGAATCCTACTCAGAAACCATTAGTACCCTAAAGTTTGCTGAGAGGGTGTCTG L T - N P T Q K P L V P - S L L R G C L - R R I L L R N H - Y P K V C - E G V W P D V E S Y S E T I S T L K F A E R V S . . . . . . 141940 GAGTGGAGTTAGGTGCAGCTCGGAATAATAAAGAAGGCCGGGGTGTTAAAGAGCTTATGG E W S - V Q L G I I K K A G V L K S L W S G V R C S S E - - R R P G C - R A Y G G V E L G A A R N N K E G R G V K E L M . : . . . . . 141880 ATCAG : GTAGCTAATTTAAAGGACACAATTGCCAAGAAGGATGAAGAAATTGGGCGGCTGC I R : - L I - R T Q L P R R M K K L G G C S : G S - F K G H N C Q E G - R N W A A A D Q : V A N L K D T I A K K D E E I G R L . . . . . . 141545 GGGTTCCCAAAAATAGTGGTAACGGTGAGAGGCGTAGTGTGAGCTCAACCAGGCATAGCT G F P K I V V T V R G V V - A Q P G I A G S Q K - W - R - E A - C E L N Q A - L R V P K N S G N G E R R S V S S T R H S . . . . . . 141485 CTGCCTCTCCAAGAAGGCAATCTTTAGGCGATCCACGAACAAACCAAATTTCTGGAGAGA L P L Q E G N L - A I H E Q T K F L E R C L S K K A I F R R S T N K P N F W R E S A S P R R Q S L G D P R T N Q I S G E . . . . . . 141425 GAAGCTCAAAACCTACTCAGAAAGCTGCTTCTGACGTGGACAATAGTTCTGAATACAGCG E A Q N L L R K L L L T W T I V L N T A K L K T Y S E S C F - R G Q - F - I Q R R S S K P T Q K A A S D V D N S S E Y S . . . . . . 141365 ACAGACAATCTGACACTGGTTCTCAGCAATCAATGGATGACTTTAGGCATCACAGAGATT T D N L T L V L S N Q W M T L G I T E I Q T I - H W F S A I N G - L - A S Q R F D R Q S D T G S Q Q S M D D F R H H R D . . . . . . 141305 TCTTTAGACAATCCAGGCTTGCTGTAGTAGATGCTGATCTAAATCTGGGTGAACACACTA S L D N P G L L - - M L I - I W V N T L L - T I Q A C C S R C - S K S G - T H - F F R Q S R L A V V D A D L N L G E H T . . . . . . 141245 ATTCAAGGGCTACTGCGAGAGGAAGTCAAAATCCTAACGAAGATGTGGTGCTTATTGGGT I Q G L L R E E V K I L T K M W C L L G F K G Y C E R K S K S - R R C G A Y W V N S R A T A R G S Q N P N E D V V L I G . . . . . . 141185 TTGATGATGCAGATTCTGAAGAGAGATTAAGTGACATATCCGATGGTGTACTCTCAATGG L M M Q I L K R D - V T Y P M V Y S Q W - - C R F - R E I K - H I R W C T L N G F D D A D S E E R L S D I S D G V L S M . . . . . . 141125 GAACTGAGACTGATGGTTCAATCAATAGTATTGTAGAGTACACTCTTTTCCCAGAAACTA E L R L M V Q S I V L - S T L F S Q K L N - D - W F N Q - Y C R V H S F P R N Y G T E T D G S I N S I V E Y T L F P E T . . . : . . . 141065 CAAAGCCACCTCCAGAGACCCCTGAGAA : GCCCTCAATTATACCTGCTAAACTTCCACGGC Q S H L Q R P L R : S P Q L Y L L N F H G K A T S R D P - E : A L N Y T C - T S T A T K P P P E T P E K : P S I I P A K L P R . . . . . . 140495 CTACACAAAAGACGGTGCAAACAGGATCTTCTCGGATGTCACTGAAGAAGAGCACCCCTA L H K R R C K Q D L L G C H - R R A P L Y T K D G A N R I F S D V T E E E H P - P T Q K T V Q T G S S R M S L K K S T P . : . . . . . 140435 AAGTCCCGA : GTTCTAAAAAACCTCCTTCCGGAAACACTTCTGCAGTTAGGTCTTCCAAAA K S R : V L K N L L P E T L L Q L G L P K S P E : F - K T S F R K H F C S - V F Q K K V P : S S K K P P S G N T S A V R S S K . . . . . . 140221 GATGGCAATAGTACAGACATTAAATTGTCGAAGAGGACAGACACTTGACGATCCTTCTTT D G N S T D I K L S K R T D T - R S F F M A I V Q T L N C R R G Q T L D D P S L R W Q - Y R H - I V E E D R H L T I L L . . . . . . 140161 GTAGATTAGACTGTAGACACGATTGTTGTGTCATCTTCCATTGCTGTGGAAGATGTGTAG V D - T V D T I V V S S S I A V E D V - - I R L - T R L L C H L P L L W K M C R C R L D C R H D C C V I F H C C G R C V . . . . . . 140101 GAACTTCAGCTTGTAGCAAACTGCACTGATCAGATTGAATTTCTTTAGGTGTATTTGCTC E L Q L V A N C T D Q I E F L - V Y L L N F S L - Q T A L I R L N F F R C I C S G T S A C S K L H - S D - I S L G V F A . . . . . . 140041 CCTGCCTCCCCCTACTCGAAGTGAAGGAGTAAAGGAGGGCGGCGTGGGGTTTGACATTGT P A S P Y S K - R S K G G R R G V - H C L P P P T R S E G V K E G G V G F D I V P C L P L L E V K E - R R A A W G L T L . . . . . . 139981 GTAGTGTGTACATGTAGCTCTCTTTATGAACTTGCTGATGCATTTTTAGGCTATGAAATA V V C T C S S L Y E L A D A F L G Y E I - C V H V A L F M N L L M H F - A M K - C S V Y M - L S L - T C - C I F R L - N . . 139921 GTGTTGACTGATAA V L T D C - L I S V D - - Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1-_PGL-18_AGS-1_PPS_1 (143001 142862,142668 142504,142415 142162,142039 141876,141600 141038,140527 140427,140272 140211) (frame '0'; 1446 bp, 482 residues) 1 NYRALNDLFN ISQSRKSSIA YEVGVQMVEI YNEQVRDLLC SDTSQKRLGI WSTTQPNGLA 61 VPDASMHPVK STANVLELMN IGLMNRAVGA TALNERSSRS HSILTVHVRG IDLETNDILR 121 GCLHLVDLAG SERVDRSEAT GDRLREAQHI NKSLSALGDV IFALAQKSSH VPYRNSKLTQ 181 VLQSSLGGQA KTLMFVQLNP DVESYSETIS TLKFAERVSG VELGAARNNK EGRGVKELMD 241 QVANLKDTIA KKDEEIGRLR VPKNSGNGER RSVSSTRHSS ASPRRQSLGD PRTNQISGER 301 SSKPTQKAAS DVDNSSEYSD RQSDTGSQQS MDDFRHHRDF FRQSRLAVVD ADLNLGEHTN 361 SRATARGSQN PNEDVVLIGF DDADSEERLS DISDGVLSMG TETDGSINSI VEYTLFPETT 421 KPPPETPEKP SIIPAKLPRP TQKTVQTGSS RMSLKKSTPK VPSSKKPPSG NTSAVRSSKR 481 WQ- PGL 19 (+ strand): 151139 155241 AGS-1 (151139 151368,152865 152931,154235 154283,154606 154650,154962 155241) SCR (e 1.000 d 0.981 a 0.992,e 1.000 d 0.967 a 0.991,e 1.000 d 0.969 a 0.862,e 1.000 d 0.990 a 0.954,e 1.000) Exon 1 151139 151368 ( 230 n); score: 1.000 Intron 1 151369 152864 (1496 n); Pd: 0.981 Pa: 0.992 Exon 2 152865 152931 ( 67 n); score: 1.000 Intron 2 152932 154234 (1303 n); Pd: 0.967 Pa: 0.991 Exon 3 154235 154283 ( 49 n); score: 1.000 Intron 3 154284 154605 ( 322 n); Pd: 0.969 Pa: 0.862 Exon 4 154606 154650 ( 45 n); score: 1.000 Intron 4 154651 154961 ( 311 n); Pd: 0.990 Pa: 0.954 Exon 5 154962 155241 ( 280 n); score: 1.000 PGS (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) SGN-E324968+ PGS (151139 151368,152865 152931,154235 154283,154606 154650,154962 155080) SGN-E324971+ PGS (151179 151368,152865 152931,154235 154283,154606 154650,154962 155208) SGN-E556545+ PGS (154612 154650,154962 155241) SGN-E237330- PGS (154962 155241) SGN-E237221- 3-phase translation of AGS-1 (+strand): . . . . . . 151139 AGAAGGAGGAAGAGAAGAATCAACTGATAACAGACGATGACAGAGATGGAGAAGAAGAAC R R R K R R I N - - Q T M T E M E K K N E G G R E E S T D N R R - Q R W R R R T K E E E K N Q L I T D D D R D G E E E . . . . . . 151199 AACAACAAAGAGGGGAATGTGGTGATCACGAAAATGCGGAATCTCAAACATCGTCGTCTT N N K E G N V V I T K M R N L K H R R L T T K R G M W - S R K C G I S N I V V F Q Q Q R G E C G D H E N A E S Q T S S S . . . . . . 151259 TAAATGGCAAAGAAATACTGAAAGCAATCGAAGTTGTGGAGAGAGATTCTATGGCGATCG - M A K K Y - K Q S K L W R E I L W R S K W Q R N T E S N R S C G E R F Y G D R L N G K E I L K A I E V V E R D S M A I . . . . . : . 151319 CCCATAGCTACACTTCACTTTTTGCTTCTCTTCGATCAACTTTATCAGAG : GTTACTAGTA P I A T L H F L L L F D Q L Y Q R : L L V P - L H F T F C F S S I N F I R : G Y - Y A H S Y T S L F A S L R S T L S E : V T S . . . . . . : 152875 CTTCTGTTGATCACATGATCTGCTTTGGTGATGCAGCAGGACGAGTTCAAGAATGTG : CAC L L L I T - S A L V M Q Q D E F K N V : H F C - S H D L L W - C S R T S S R M C : T T S V D H M I C F G D A A G R V Q E C : A . . . . . : . 154238 TTGATGCAGCAACTAAAGGAAACCGTTACATAAACTCTTGCTTAAG : ATTGAACGAGGAAA L M Q Q L K E T V T - T L A - : D - T R K - C S N - R K P L H K L L L K : I E R G N L D A A T K G N R Y I N S C L R : L N E E . . . . : . . 154620 TGAAAGGAATTGATAATCTTGCAACCCAGCT : GAAAATCATCCGGAGAAACGTAGATGCAT - K E L I I L Q P S : - K S S G E T - M H E R N - - S C N P A : E N H P E K R R C I M K G I D N L A T Q L : K I I R R N V D A . . . . . . 154991 TGGACTCGGCTGTAAATAGGCTAGTTCGTTTCCCATGAGTGTGGATCTGTCTTGATGGAC W T R L - I G - F V S H E C G S V L M D G L G C K - A S S F P M S V D L S - W T L D S A V N R L V R F P - V W I C L D G . . . . . . 155051 GATGTCAACTAGGTGAGCTGCACATATTTTGTGGTGCTTTATATATGAGTGAGATGCCAG D V N - V S C T Y F V V L Y I - V R C Q M S T R - A A H I L W C F I Y E - D A S R C Q L G E L H I F C G A L Y M S E M P . . . . . . 155111 TTAAGTCTTGCTTCTTTGAAGGTTTAAGAATAAAACAGTGTGTAATTGAATGAATTGCAA L S L A S L K V - E - N S V - L N E L Q - V L L L - R F K N K T V C N - M N C K V K S C F F E G L R I K Q C V I E - I A . . . . . . 155171 GGCTGATACATTTTCTAACCCATAGAAAAGCTTTCACCATTGTTGCTTTTGCATACAATT G - Y I F - P I E K L S P L L L L H T I A D T F S N P - K S F H H C C F C I Q F R L I H F L T H R K A F T I V A F A Y N . . 155231 TTTACCTCACT F T S L P H F Y L T Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-19_AGS-1_PPS_1 (151141 151368,152865 152931,154235 154283,154606 154650,154962 155028) (frame '0'; 453 bp, 151 residues) 1 KEEEKNQLIT DDDRDGEEEQ QQRGECGDHE NAESQTSSSL NGKEILKAIE VVERDSMAIA 61 HSYTSLFASL RSTLSEVTST SVDHMICFGD AAGRVQECAL DAATKGNRYI NSCLRLNEEM 121 KGIDNLATQL KIIRRNVDAL DSAVNRLVRF P- AGS-2 (151160 151804) SCR (e 0.991) Exon 1 151160 151804 ( 645 n); score: 0.991 PGS (151160 151804) SGN-E314977+ 3-phase translation of AGS-2 (+strand): . . . . . . 151160 AACTGATAACAGACGATGACAGAGATGGAGAAGAAGAACAACAACAAAGAGGGGAATGTG N - - Q T M T E M E K K N N N K E G N V T D N R R - Q R W R R R T T T K R G M W L I T D D D R D G E E E Q Q Q R G E C . . . . . . 151220 GTGATCACGAAAATGCGGAATCTCAAACATCGTCGTCTTTAAATGGCAAAGAAATACTGA V I T K M R N L K H R R L - M A K K Y - - S R K C G I S N I V V F K W Q R N T E G D H E N A E S Q T S S S L N G K E I L . . . . . . 151280 AAGCAATCGAAGTTGTGGAGAGAGATTCTATGGCGATCGCCCATAGCTACACTTCACTTT K Q S K L W R E I L W R S P I A T L H F S N R S C G E R F Y G D R P - L H F T F K A I E V V E R D S M A I A H S Y T S L . . . . . . 151340 TTGCTTCTCTTCGATCAACTTTATCAGAGGTTTCTTCTTTCTGTCCTTAATTCTTGCATT L L L F D Q L Y Q R F L L S V L N S C I C F S S I N F I R G F F F L S L I L A F F A S L R S T L S E V S S F C P - F L H . . . . . . 151400 TCCTCATAATCTTAGTACTCGGTGGTGTTGAGGCCACTGCTGCAAATAGTGGCGTAGCTT S S - S - Y S V V L R P L L Q I V A - L P H N L S T R W C - G H C C K - W R S F F L I I L V L G G V E A T A A N S G V A . . . . . . 151460 TGAGATAGTGTTATCTGAAAGTAATTATCTTTAGTCCTTCATCATGTTCAGTGTTTGGCA - D S V I - K - L S L V L H H V Q C L A E I V L S E S N Y L - S F I M F S V W H L R - C Y L K V I I F S P S S C S V F G . . . . . . 151520 CGTTTCTTTTTAATTTGGTTACTGATAAAAAGAGAGTCTTTTTTGTTTTTTTAATTTGGT R F F L I W L L I K R E S F L F F - F G V S F - F G Y - - K E S L F C F F N L V T F L F N L V T D K K R V F F V F L I W . . . . . . 151580 TTGAGATGATTGTATGTCAGTGGAGGGATGATTGTGAGATTTAGAGAATCCTCAATTTAT L R - L Y V S G G M I V R F R E S S I Y - D D C M S V E G - L - D L E N P Q F I F E M I V C Q W R D D C E I - R I L N L . . . . . . 151640 AATGAACTGGAGTGGATGTTGAGTATAGATATAGCTTATCTTAGTTAGTTTGGGAATTAA N E L E W M L S I D I A Y L S - F G N - M N W S G C - V - I - L I L V S L G I K - - T G V D V E Y R Y S L S - L V W E L . . . . . . 151700 GCGAAGAAGTAGTAGTTGGATAGAAAAGCATGTTTAAGGTGATCAACGAGCTGCATGGTG A K K - - L D R K A C L R - S T S C M V R R S S S W I E K H V - G D Q R A A W - S E E V V V G - K S M F K V I N E L H G . . . . . 151760 ATCTAAGCTCTTTGCCTGCTTTTTGATTGTGCATTTTGCTTGTAA I - A L C L L F D C A F C L - S K L F A C F L I V H F A C D L S S L P A F - L C I L L V Maximal non-overlapping open reading frames (>= 64 codons): >C08HBa0251H04.1+_PGL-19_AGS-2_PPS_1 (151162 151389) (frame '0'; 225 bp, 75 residues) 1 LITDDDRDGE EEQQQRGECG DHENAESQTS SSLNGKEILK AIEVVERDSM AIAHSYTSLF 61 ASLRSTLSEV SSFCP- 3-phase translation of AGS-2 (-strand): . . . . . . 151804 TTACAAGCAAAATGCACAATCAAAAAGCAGGCAAAGAGCTTAGATCACCATGCAGCTCGT L Q A K C T I K K Q A K S L D H H A A R Y K Q N A Q S K S R Q R A - I T M Q L V T S K M H N Q K A G K E L R S P C S S . . . . . . 151744 TGATCACCTTAAACATGCTTTTCTATCCAACTACTACTTCTTCGCTTAATTCCCAAACTA - S P - T C F S I Q L L L L R L I P K L D H L K H A F L S N Y Y F F A - F P N - L I T L N M L F Y P T T T S S L N S Q T . . . . . . 151684 ACTAAGATAAGCTATATCTATACTCAACATCCACTCCAGTTCATTATAAATTGAGGATTC T K I S Y I Y T Q H P L Q F I I N - G F L R - A I S I L N I H S S S L - I E D S N - D K L Y L Y S T S T P V H Y K L R I . . . . . . 151624 TCTAAATCTCACAATCATCCCTCCACTGACATACAATCATCTCAAACCAAATTAAAAAAA S K S H N H P S T D I Q S S Q T K L K K L N L T I I P P L T Y N H L K P N - K N L - I S Q S S L H - H T I I S N Q I K K . . . . . . 151564 CAAAAAAGACTCTCTTTTTATCAGTAACCAAATTAAAAAGAAACGTGCCAAACACTGAAC Q K R L S F Y Q - P N - K E T C Q T L N K K D S L F I S N Q I K K K R A K H - T T K K T L F L S V T K L K R N V P N T E . . . . . . 151504 ATGATGAAGGACTAAAGATAATTACTTTCAGATAACACTATCTCAAAGCTACGCCACTAT M M K D - R - L L S D N T I S K L R H Y - - R T K D N Y F Q I T L S Q S Y A T I H D E G L K I I T F R - H Y L K A T P L . . . . . . 151444 TTGCAGCAGTGGCCTCAACACCACCGAGTACTAAGATTATGAGGAAATGCAAGAATTAAG L Q Q W P Q H H R V L R L - G N A R I K C S S G L N T T E Y - D Y E E M Q E L R F A A V A S T P P S T K I M R K C K N - . . . . . . 151384 GACAGAAAGAAGAAACCTCTGATAAAGTTGATCGAAGAGAAGCAAAAAGTGAAGTGTAGC D R K K K P L I K L I E E K Q K V K C S T E R R N L - - S - S K R S K K - S V A G Q K E E T S D K V D R R E A K S E V - . . . . . . 151324 TATGGGCGATCGCCATAGAATCTCTCTCCACAACTTCGATTGCTTTCAGTATTTCTTTGC Y G R S P - N L S P Q L R L L S V F L C M G D R H R I S L H N F D C F Q Y F F A L W A I A I E S L S T T S I A F S I S L . . . . . . 151264 CATTTAAAGACGACGATGTTTGAGATTCCGCATTTTCGTGATCACCACATTCCCCTCTTT H L K T T M F E I P H F R D H H I P L F I - R R R C L R F R I F V I T T F P S L P F K D D D V - D S A F S - S P H S P L . . . . . 151204 GTTGTTGTTCTTCTTCTCCATCTCTGTCATCGTCTGTTATCAGTT V V V L L L H L C H R L L S V L L F F F S I S V I V C Y Q C C C S S S P S L S S S V I S Maximal non-overlapping open reading frames (>= 64 codons): none PGL 20 (+ strand): 157358 157697 AGS-1 (157358 157697) SCR (e 1.000) Exon 1 157358 157697 ( 340 n); score: 1.000 PGS (157358 157697) SGN-E322356+ 3-phase translation of AGS-1 (+strand): . . . . . . 157358 CTGTTTTGGCCTTGTTTCTTGAAATCTGAAACTAAAAAATTCTCTTTGTATGGTCCAATA L F W P C F L K S E T K K F S L Y G P I C F G L V S - N L K L K N S L C M V Q Y V L A L F L E I - N - K I L F V W S N . . . . . . 157418 TGGTTGTGGTCCTGAGAACTAAGATCCCATTTGGGATCAAGATTCAAGATTTATGCTTTC W L W S - E L R S H L G S R F K I Y A F G C G P E N - D P I W D Q D S R F M L S M V V V L R T K I P F G I K I Q D L C F . . . . . . 157478 TTGGTGTTTTATGATTTTGGTGTCGTTTTTTAGAATTTTGAGCTGATTTTTTCTTCACTG L V F Y D F G V V F - N F E L I F S S L W C F M I L V S F F R I L S - F F L H C L G V L - F W C R F L E F - A D F F F T . . . . . . 157538 TCATTTTGGTGAGACAAAAGATTGAAGCTTTGAAAATGGCTATGGGACACCAAGATCAAG S F W - D K R L K L - K W L W D T K I K H F G E T K D - S F E N G Y G T P R S R V I L V R Q K I E A L K M A M G H Q D Q . . . . . . 157598 ATGGAGTCCCAGGCAACTTGAGAAAGCAACTTGCTCTTGCTGTTAGAGGCATTCAATGGA M E S Q A T - E S N L L L L L E A F N G W S P R Q L E K A T C S C C - R H S M E D G V P G N L R K Q L A L A V R G I Q W . . . . 157658 GCTATGCAATCTTCTGGTCAACTGCAGTTACACAACCAGG A M Q S S G Q L Q L H N Q L C N L L V N C S Y T T R S Y A I F W S T A V T Q P Maximal non-overlapping open reading frames (>= 64 codons): none 3-phase translation of AGS-1 (-strand): . . . . . . 157697 CCTGGTTGTGTAACTGCAGTTGACCAGAAGATTGCATAGCTCCATTGAATGCCTCTAACA P G C V T A V D Q K I A - L H - M P L T L V V - L Q L T R R L H S S I E C L - Q W L C N C S - P E D C I A P L N A S N . . . . . . 157637 GCAAGAGCAAGTTGCTTTCTCAAGTTGCCTGGGACTCCATCTTGATCTTGGTGTCCCATA A R A S C F L K L P G T P S - S W C P I Q E Q V A F S S C L G L H L D L G V P - S K S K L L S Q V A W D S I L I L V S H . . . . . . 157577 GCCATTTTCAAAGCTTCAATCTTTTGTCTCACCAAAATGACAGTGAAGAAAAAATCAGCT A I F K A S I F C L T K M T V K K K S A P F S K L Q S F V S P K - Q - R K N Q L S H F Q S F N L L S H Q N D S E E K I S . . . . . . 157517 CAAAATTCTAAAAAACGACACCAAAATCATAAAACACCAAGAAAGCATAAATCTTGAATC Q N S K K R H Q N H K T P R K H K S - I K I L K N D T K I I K H Q E S I N L E S S K F - K T T P K S - N T K K A - I L N . . . . . . 157457 TTGATCCCAAATGGGATCTTAGTTCTCAGGACCACAACCATATTGGACCATACAAAGAGA L I P N G I L V L R T T T I L D H T K R - S Q M G S - F S G P Q P Y W T I Q R E L D P K W D L S S Q D H N H I G P Y K E . . . . 157397 ATTTTTTAGTTTCAGATTTCAAGAAACAAGGCCAAAACAG I F - F Q I S R N K A K T F F S F R F Q E T R P K Q N F L V S D F K K Q G Q N Maximal non-overlapping open reading frames (>= 64 codons): none ... finished at: Tue Jul 25 11:46:18 2006