This is the protein prediction files for the Nicotiana tabacum unigenes dataset (build_n=2 and date=2008_07_17) using the EstScan (version 3.0) software (http://estscan.sourceforge.net/).

The model for the ESTScan was built based on 483 EMBL tomato (lycopersicon esculentum) nuclear coding sequences using the software provided with ESTScan.  

After we built the model, we evaluated the model in terms of its ability to determine the right frame, identify the coding region and fix the frame shift error.  
The test sequences came from published tomato cDNA sequences with known start and stop, published tomato cDNA sequences with deliberately introduced frame shift errors and tomato ESTs with coding regions identified through alignment to arabidopsis homolog. 
It turned out that ESTScan, with the tomato model we built, was able to find the frame with high accuracy.  Most of the coding regions identified by ESTScan were correct.  
However, it might miss 3 to 5 amino acids at both ends.  
Especially, when the non coding region was short (less than 30 nucleotide), ESTScan might not be able to distinguish it from the coding region.  
ESTScan fixed the frame shift errors in most of the test sequences, not guaranteed at exactly the insert/deletion position but usually within 10 nuceotides up or down stream of the shift error.

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Report:
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Input options:
        * Organism dataset: Nicotiana tabacum
        * Unigene build id: 42
        * Unigene build number for this organism: 2
        * Protein prediction method: estscan
Output data:
        * cds sequences:
                - cds fasta file: Nicotiana_tabacum_cds_predicted_by_estscan_2008_07_17.fasta
                - number of sequences: 80117
                - total bases: 40799580
                - average sequences length: 509
                - maximum sequence length: 6291
                - minimum sequence length: 51
        * protein sequences:
                - protein fasta file: Nicotiana_tabacum_protein_predicted_by_estscan_2008_07_17.fasta
                - number of sequences: 80117
                - total aminoacids: 13599860
                - average sequences length: 169
                - maximum sequence length: 2097
                - minimum sequence length: 17
        * cds unigene mapping:
                - cds-unigene map tab file: Nicotiana_tabacum_unigene_cds_mapping_for_estscan_2008_07_17.membermap.tab

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