BLASTP 2.11.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: N.tabacum BX protein sequences
           86,009 sequences; 32,278,096 total letters



Query= Untitled_sequence

Length=500
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

mRNA_3529_cdsmRNA_3529 gene_2045|id=ATMG00580.1:evalue=2e-87:anno...  104     8e-26
mRNA_3528_cdsmRNA_3528 gene_2045|id=ATMG00580.1:evalue=2e-87:anno...  104     8e-26


>mRNA_3529_cds mRNA_3529 gene_2045|id=ATMG00580.1:evalue=2e-87:annot='NADH dehydrogenase 
subunit 4';id=Solyc11g030570.1.1:evalue=2e-34:annot='NADH-ubiquinone 
oxidoreductase chain 4'
Length=172

 Score = 104 bits (260),  Expect = 8e-26, Method: Compositional matrix adjust.
 Identities = 51/127 (40%), Positives = 80/127 (63%), Gaps = 0/127 (0%)

Query  194  TLNFETSVNQSYPVVLEIIFYIGFFIAFAVKSPIIPLHTWLPDTHGEAHYSTCMLLAGIL  253
            T + + S+   +    +I  +I  F +FAVK P++P+H WLP+ H EA  +  ++LAGI 
Sbjct  14   TTDLQISLTTEFSERRQIFLWIASFASFAVKVPMVPVHIWLPEAHVEAPTAGSVILAGIA  73

Query  254  LKMGAYGLIRINMELLPHAHSIFSPWLMIIGTIQIIYAALTSLGQRNLKKRIAYSSVSHM  313
            LK+G YG +R ++ + P A    +P++  +  I IIY + T+L Q +LKK IAYSSV+HM
Sbjct  74   LKLGTYGFLRFSIPMFPEATLCSTPFIYTLSAIAIIYTSSTTLRQIDLKKIIAYSSVAHM  133

Query  314  GFIIIGI  320
              + IG+
Sbjct  134  NLVTIGM  140


>mRNA_3528_cds mRNA_3528 gene_2045|id=ATMG00580.1:evalue=2e-87:annot='NADH dehydrogenase 
subunit 4';id=Solyc11g030570.1.1:evalue=2e-34:annot='NADH-ubiquinone 
oxidoreductase chain 4'
Length=172

 Score = 104 bits (260),  Expect = 8e-26, Method: Compositional matrix adjust.
 Identities = 51/127 (40%), Positives = 80/127 (63%), Gaps = 0/127 (0%)

Query  194  TLNFETSVNQSYPVVLEIIFYIGFFIAFAVKSPIIPLHTWLPDTHGEAHYSTCMLLAGIL  253
            T + + S+   +    +I  +I  F +FAVK P++P+H WLP+ H EA  +  ++LAGI 
Sbjct  14   TTDLQISLTTEFSERRQIFLWIASFASFAVKVPMVPVHIWLPEAHVEAPTAGSVILAGIA  73

Query  254  LKMGAYGLIRINMELLPHAHSIFSPWLMIIGTIQIIYAALTSLGQRNLKKRIAYSSVSHM  313
            LK+G YG +R ++ + P A    +P++  +  I IIY + T+L Q +LKK IAYSSV+HM
Sbjct  74   LKLGTYGFLRFSIPMFPEATLCSTPFIYTLSAIAIIYTSSTTLRQIDLKKIIAYSSVAHM  133

Query  314  GFIIIGI  320
              + IG+
Sbjct  134  NLVTIGM  140



Lambda      K        H        a         alpha
   0.332    0.147    0.460    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 8898671340


  Database: N.tabacum BX protein sequences
    Posted date:  Mar 21, 2024  3:19 PM
  Number of letters in database: 32,278,096
  Number of sequences in database:  86,009



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40