BLASTP 2.11.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: N.benthamiana Genome v1.0.1 predicted proteins
           57,140 sequences; 18,711,326 total letters



Query= Untitled_sequence

Length=622
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

Niben101Scf00593g04010.1AT1G18870.1 *-** isochorismate synthase 2...  243     5e-76
Niben101Scf05166g06006.1AT1G18870.1 *-** isochorismate synthase 2...  239     1e-74
Niben101Scf11531g01010.1AT1G18870.1 *-*- isochorismate synthase 2...  74.3    6e-14


>Niben101Scf00593g04010.1 AT1G18870.1 *-** isochorismate synthase 2 LENGTH=562 IPR004561 
(Isochorismate synthase) GO:0008909 (isochorismate synthase 
activity), GO:0009058 (biosynthetic process)
Length=250

 Score = 243 bits (619),  Expect = 5e-76, Method: Compositional matrix adjust.
 Identities = 121/241 (50%), Positives = 162/241 (67%), Gaps = 12/241 (5%)

Query  102  QVPIDQQIGAIDWLQAQNEIQ-PRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRN  160
            +VP+++QI A++WL AQN +  PRCFFS R      ++             NGT      
Sbjct  9    KVPVEEQIEALEWLHAQNHVLLPRCFFSGRRAAADSEMCF-----------NGTSHPSSK  57

Query  161  LVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFG  220
            +VSVAG+GSAVFF  L PFS DDWR+IRRFLS   PLIRAYG +RFD    I  EW  FG
Sbjct  58   VVSVAGVGSAVFFTHLRPFSFDDWRAIRRFLSKKCPLIRAYGAIRFDATANIGPEWNAFG  117

Query  221  AFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSL  280
            +FYF VPQVEF+E  GSS++AAT+AWD+ +S T + AIEALQ T+ QVSSV+M+++ +  
Sbjct  118  SFYFMVPQVEFDELEGSSVIAATVAWDNAVSCTYQMAIEALQATIWQVSSVLMRVQKKIS  177

Query  281  GVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQL  340
               +L+  HVP K ++  AV++AL+ I +    L KVVLAR+SR++T  DIDP+ WL+ L
Sbjct  178  RSHLLASTHVPGKASWDQAVKRALQKIRRNDPMLIKVVLARSSRVVTAADIDPLTWLSCL  237

Query  341  Q  341
            +
Sbjct  238  K  238


>Niben101Scf05166g06006.1 AT1G18870.1 *-** isochorismate synthase 2 LENGTH=562 IPR004561 
(Isochorismate synthase) GO:0008909 (isochorismate synthase 
activity), GO:0009058 (biosynthetic process)
Length=252

 Score = 239 bits (611),  Expect = 1e-74, Method: Compositional matrix adjust.
 Identities = 122/241 (51%), Positives = 166/241 (69%), Gaps = 12/241 (5%)

Query  102  QVPIDQQIGAIDWLQAQNEIQ-PRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRN  160
            +VPI++QI A++WL AQN +  PRCFFS R     P++             NGT  S   
Sbjct  9    KVPIEEQIEALEWLHAQNHVLLPRCFFSGRRPAVEPEMCF-----------NGTSHSSSK  57

Query  161  LVSVAGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFG  220
            +VSVAG+GSAVFF  L PFS DDWR+IRRFLS   PLIRAYG +RFD    I  EW  FG
Sbjct  58   VVSVAGVGSAVFFTHLRPFSFDDWRAIRRFLSKKCPLIRAYGAIRFDATANIGPEWNAFG  117

Query  221  AFYFSVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSL  280
            +FYF VPQVEF+E  GSS++AAT+AWD+ +S T + +IEALQ T+ QVSSV+M+++ +  
Sbjct  118  SFYFMVPQVEFDELEGSSVIAATVAWDNAVSCTYQRSIEALQATIWQVSSVLMRVQKKIS  177

Query  281  GVSVLSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQL  340
               +L+  HVP K ++  AV++AL++I + +  L KVVLAR++R++T  DIDP+ WL+ L
Sbjct  178  RSHILASTHVPGKASWDQAVKRALQIIRRNNPMLIKVVLARSTRVVTAADIDPLTWLSCL  237

Query  341  Q  341
            +
Sbjct  238  K  238


>Niben101Scf11531g01010.1 AT1G18870.1 *-*- isochorismate synthase 2 LENGTH=562  
Length=362

 Score = 74.3 bits (181),  Expect = 6e-14, Method: Compositional matrix adjust.
 Identities = 65/244 (27%), Positives = 114/244 (47%), Gaps = 50/244 (20%)

Query  59   LGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVPIDQQIGAIDWLQAQ  118
            + T  TRT++  L+     ER+  AV+ELK++PP  SSG++R +V +     +++W   Q
Sbjct  74   VSTCITRTLSPALTLEQGLERIKEAVAELKAKPPCCSSGMLRFRVAVPPSTKSLNWFCCQ  133

Query  119  NE---IQPRCFFSR-RSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSVAGIGSAVFFR  174
             +   + P+ F S+ R +     L+L      G+  G            + GIG+A+ F 
Sbjct  134  PKSSGVFPQFFLSKERENQTFKSLVL------GHTRG------------IFGIGAAISFE  175

Query  175  DLDPFSHDDWRSIRRFLSSTSPLIRAYG--GMRFDPNGKIAVEWEPFGAFYFSVPQ----  228
                 +  ++    R  +  S  + AYG   +RFD     +++ E  G+FYF +PQ    
Sbjct  176  GFS--ASQEYGEFGRCCAVESTPVVAYGFLNLRFDTISS-SMKQEA-GSFYFFIPQVKMS  231

Query  229  -----------------VEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSV  271
                             VE  EF G+S L+ T+AW   +  T E A++  + ++LQ+ ++
Sbjct  232  LFAWPWYRFFLYSPKCLVELEEFEGASTLSTTVAWHSCIC-TFEEALQVYESSLLQLLTI  290

Query  272  VMKL  275
             + L
Sbjct  291  NIML  294



Lambda      K        H        a         alpha
   0.320    0.134    0.398    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 6487633190


  Database: N.benthamiana Genome v1.0.1 predicted proteins
    Posted date:  Mar 21, 2024  4:04 PM
  Number of letters in database: 18,711,326
  Number of sequences in database:  57,140



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40