BLASTN 2.11.0+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: N.benthamiana Genome v1.0.1 predicted cDNA 57,140 sequences; 82,582,885 total letters Query= Untitled_sequence Length=696 Score E Sequences producing significant alignments: (Bits) Value Niben101Scf06369g03006.1sp|O23970|GPX1_HELAN *-** Glutathione per... 1286 0.0 Niben101Scf05417g01007.1sp|O23970|GPX1_HELAN *-** Glutathione per... 590 1e-167 Niben101Scf01339g06015.1sp|O23970|GPX1_HELAN *-** Glutathione per... 237 2e-61 Niben101Ctg15145g00003.1sp|O23970|GPX1_HELAN *-** Glutathione per... 158 1e-37 >Niben101Scf06369g03006.1 sp|O23970|GPX1_HELAN *-** Glutathione peroxidase 1 IPR000889 (Glutathione peroxidase), IPR012336 (Thioredoxin-like fold), IPR029760 (Glutathione peroxidase conserved site) GO:0004602 (glutathione peroxidase activity), GO:0006979 (response to oxidative stress), GO:0055114 (oxidation-reduction process) Length=1080 Score = 1286 bits (696), Expect = 0.0 Identities = 696/696 (100%), Gaps = 0/696 (0%) Strand=Plus/Plus Query 1 ATGCTTTATTCTGCAACTCGTGTTCTCATACCAAGAAAAAATCTCAGTTTCCTTAGGCAA 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 71 ATGCTTTATTCTGCAACTCGTGTTCTCATACCAAGAAAAAATCTCAGTTTCCTTAGGCAA 130 Query 61 TTTTCTTCGATTATAAAGCCAACCCATTTCAATTCTGTATCTATTAAGCCGATCCAAACA 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 131 TTTTCTTCGATTATAAAGCCAACCCATTTCAATTCTGTATCTATTAAGCCGATCCAAACA 190 Query 121 GTTCTTTCCAATTCTATTGTTTCCGCTAAGAGGTTTGAGTTGTTTTGTTTAAGATCAGAT 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 191 GTTCTTTCCAATTCTATTGTTTCCGCTAAGAGGTTTGAGTTGTTTTGTTTAAGATCAGAT 250 Query 181 TATAGTACCATGGCCAGTCAATCCAGCAAGCCTCAATCTATTTACGATTTCACTGTCAAG 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 251 TATAGTACCATGGCCAGTCAATCCAGCAAGCCTCAATCTATTTACGATTTCACTGTCAAG 310 Query 241 GATGCTAAGGGTAATGATGTCAATCTCAGCATTTACAAGGGAAAGGTCCTTATTATTGTC 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 311 GATGCTAAGGGTAATGATGTCAATCTCAGCATTTACAAGGGAAAGGTCCTTATTATTGTC 370 Query 301 AATGTTGCATCACATGGTCTGACAAATTCGAACTATACTGATATGACTGAGATATACAAG 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 371 AATGTTGCATCACATGGTCTGACAAATTCGAACTATACTGATATGACTGAGATATACAAG 430 Query 361 AAGTACAAGGATCAAGGTCTGGAGATTCTTGCATTCCCTTGCAACCAGTTTGGTGGGCAG 420 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 431 AAGTACAAGGATCAAGGTCTGGAGATTCTTGCATTCCCTTGCAACCAGTTTGGTGGGCAG 490 Query 421 GAACCTGGAAGCATTGAAGAGATCCAGAACATGGTTTGCACTCGCTTCAAGGCTGAGTAC 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 491 GAACCTGGAAGCATTGAAGAGATCCAGAACATGGTTTGCACTCGCTTCAAGGCTGAGTAC 550 Query 481 CCCATATTTGATAAGGTTGACGTGAATGGTGATAATGCTGCTCCACTGTATAAGTTCTTG 540 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 551 CCCATATTTGATAAGGTTGACGTGAATGGTGATAATGCTGCTCCACTGTATAAGTTCTTG 610 Query 541 AAATCAAGCAAAGGTGGGTTCTTTGGAGATAGTATCAAGTGGAACTTCTCCAAATTCCTT 600 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 611 AAATCAAGCAAAGGTGGGTTCTTTGGAGATAGTATCAAGTGGAACTTCTCCAAATTCCTT 670 Query 601 GTTGACAAAGAAGGAAACGTCGTTGATCGCTACTCTCCAACGACTGCTCCAGCTAGCATG 660 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 671 GTTGACAAAGAAGGAAACGTCGTTGATCGCTACTCTCCAACGACTGCTCCAGCTAGCATG 730 Query 661 GAGAAGGATATCAAGAAACTTTTGGGGGTTGCTTAA 696 |||||||||||||||||||||||||||||||||||| Sbjct 731 GAGAAGGATATCAAGAAACTTTTGGGGGTTGCTTAA 766 >Niben101Scf05417g01007.1 sp|O23970|GPX1_HELAN *-** Glutathione peroxidase 1 IPR000889 (Glutathione peroxidase), IPR012336 (Thioredoxin-like fold), IPR029759 (Glutathione peroxidase active site) GO:0004602 (glutathione peroxidase activity), GO:0006979 (response to oxidative stress), GO:0055114 (oxidation-reduction process) Length=478 Score = 590 bits (319), Expect = 1e-167 Identities = 361/381 (95%), Gaps = 3/381 (1%) Strand=Plus/Plus Query 1 ATGCTTTATTCTGCAACTCGTGTTCTCATACCAAGAAAAAATCTCAGTTTCCTTAGGCAA 60 ||||||||||||| |||||||||||||||||||||||||||||||| ||||||||||||| Sbjct 53 ATGCTTTATTCTGTAACTCGTGTTCTCATACCAAGAAAAAATCTCAATTTCCTTAGGCAA 112 Query 61 TTTTCTTCGATTATAAAGCCAACCCATTTCAATTCTGTATCTATTAAGCCGATCCAAACA 120 ||||||||||| |||| ||||||||||||||||||||||||||| |||||||||||||| Sbjct 113 TTTTCTTCGATCCTAAAACCAACCCATTTCAATTCTGTATCTATTCAGCCGATCCAAACA 172 Query 121 GTTCTTTCCAATTCTATTGTTTCCGCTAAGAGGTTTGAGTTGTTTTGTTTAAGATCAGAT 180 ||||||||||||||| |||||||||||||||| ||||||||||||||||||||||||||| Sbjct 173 GTTCTTTCCAATTCTTTTGTTTCCGCTAAGAGATTTGAGTTGTTTTGTTTAAGATCAGAT 232 Query 181 TATAGTACCATGGCCAGTCAATCCAGCAAGCCTCAATCTATTTACGATTTCACTGTCAAG 240 |||||||||||||||||||||||||||||||||||||||||||| || |||||||||||| Sbjct 233 TATAGTACCATGGCCAGTCAATCCAGCAAGCCTCAATCTATTTATGACTTCACTGTCAAG 292 Query 241 GATGCTAAGGGTAATGATGTCAATCTCAGCATTTACAAGGGAAAGGTCCTTATTATTGTC 300 |||||||||||||||||||| ||||||||||||||||||||||||||||||| |||||| Sbjct 293 GATGCTAAGGGTAATGATGTTGATCTCAGCATTTACAAGGGAAAGGTCCTTATCATTGTC 352 Query 301 AATGTTGCATCACA-TG-GTC-TGACAAATTCGAACTATACTGATATGACTGAGATATAC 357 |||||||||||||| || | | |||||||||||||||||||||| |||| ||||||||| Sbjct 353 AATGTTGCATCACAGTGTGGCTTGACAAATTCGAACTATACTGAATTGACCGAGATATAC 412 Query 358 AAGAAGTACAAGGATCAAGGT 378 ||||||||||||||||||||| Sbjct 413 AAGAAGTACAAGGATCAAGGT 433 >Niben101Scf01339g06015.1 sp|O23970|GPX1_HELAN *-** Glutathione peroxidase 1 IPR000889 (Glutathione peroxidase), IPR012336 (Thioredoxin-like fold), IPR029759 (Glutathione peroxidase active site), IPR029760 (Glutathione peroxidase conserved site) GO:0004602 (glutathione peroxidase activity), GO:0006979 (response to oxidative stress), GO:0055114 (oxidation-reduction process) Length=818 Score = 237 bits (128), Expect = 2e-61 Identities = 317/409 (78%), Gaps = 9/409 (2%) Strand=Plus/Plus Query 215 AATCTATTTACGATTTCACTGTCAAGGATGCTA-AGGGTAATGATGTCAATCTCAGCATT 273 |||| |||||||||||||||||||||||| || ||| ||||| || | | |||| | Sbjct 239 AATCCATTTACGATTTCACTGTCAAGGAT-ATACTGGGAAATGAGGTACCTTTGAGCAAT 297 Query 274 TACAAGGGAAAGGTCCTTATTATTGTCAATGTTGCATCA-CA--TGGTCTGACAAATTCG 330 || | ||||||||| ||| |||||||||||||||||||| | |||| | ||| |||| Sbjct 298 TATAGGGGAAAGGTTCTTCTTATTGTCAATGTTGCATCAAAATGTGGTTTAACAGATTCA 357 Query 331 AACTATACTGATATGACTGAGATATACAAGAAGTACAAGGATCAAGGTCTGGAGATTCTT 390 ||||| | || ||| | | || |||||||||| |||||||| | || ||| | Sbjct 358 AACTACAAGGAGCTGAATATTCTGTATGAGAAGTACAAAGATCAAGGATTTGAAATTTTA 417 Query 391 GCATTCCCTTGCAACCAGTT-TGGTGGGCAGGAACCTGGAAGCATTGAAGAGATCCAG-A 448 ||||| ||||| |||||||| | || |||| |||||||||| | ||| ||||| ||| | Sbjct 418 GCATTTCCTTGTAACCAGTTCTTGT-GGCAAGAACCTGGAACAAATGAGGAGATTCAGGA 476 Query 449 ACATGGTTTGCACTCGCTTCAAGGCTGAGTACCCCATATTTGATAAGGTTGACGTGAATG 508 | | || ||||| | ||||| ||||| | ||| ||||||| |||||||| || || | Sbjct 477 AACT-GTATGCACCAGGTTCAAAGCTGAATTCCCTGTATTTGAAAAGGTTGATGTAAACG 535 Query 509 GTGATAATGCTGCTCCACTGTATAAGTTCTTGAAATCAAGCAAAGGTGGGTTCTTTGGAG 568 | |||||||| || ||||| | |||||||| |||||| ||||| || ||| ||||| Sbjct 536 GGGATAATGCAGCACCACTTTTCAAGTTCTTAAAATCAGAAAAAGGCGGTTTCCTTGGAA 595 Query 569 ATAGTATCAAGTGGAACTTCTCCAAATTCCTTGTTGACAAAGAAGGAAA 617 || ||| |||||||||||| |||||||||||||| ||||||||||||| Sbjct 596 ATGCTATTAAGTGGAACTTCACCAAATTCCTTGTTAACAAAGAAGGAAA 644 >Niben101Ctg15145g00003.1 sp|O23970|GPX1_HELAN *-** Glutathione peroxidase 1 IPR000889 (Glutathione peroxidase), IPR012336 (Thioredoxin-like fold), IPR029759 (Glutathione peroxidase active site), IPR029760 (Glutathione peroxidase conserved site) GO:0004602 (glutathione peroxidase activity), GO:0006979 (response to oxidative stress), GO:0055114 (oxidation-reduction process) Length=798 Score = 158 bits (85), Expect = 1e-37 Identities = 337/460 (73%), Gaps = 12/460 (3%) Strand=Plus/Plus Query 226 GATTTCACTGTCAAGGATGCTAAGGGTAATGATGTCAATCTCAGCATTTACAAGGGAAAG 285 || |||||||| |||||| | ||||| || || | |||| || | ||||| |||||| Sbjct 175 GACTTCACTGTGAAGGATTCAAAGGGAAACTATATAGATCTTAGTAGCTACAAAGGAAAG 234 Query 286 GTCCTTATTATTGTCAATGTTGCATC--A-CATGGTCTGACAAATTCGAACTATACTGAT 342 ||||| |||| ||||||||||| || | ||| |||| || || ||||| | || Sbjct 235 GTCCTACTTATCGTCAATGTTGCTTCCAAGTGTGGGATGACTAACTCAAACTACAAAGAA 294 Query 343 ATGACTGAGATATACAAGAAGTACAAGGATCAAGGTCTGGAGATTCTTGCATTCCCTTGC 402 | | | |||| |||||||||| |||||||| ||||| || | ||||| |||||| Sbjct 295 CTCAACCAATTATATGAGAAGTACAAAGATCAAGGCCTGGAAATATTGGCATTTCCTTGC 354 Query 403 AACCAGTTTGGTGGGCAGGAACCTGGAAGCATTGAAGAGATC-CAGAACATGGTTTGCAC 461 || || ||||| | | | ||||| || |||| ||| ||||| || || | ||||| || Sbjct 355 AATCAATTTGGCGAGGAAGAACCAGGGAGCAATGATCAGATCTTAG-ACTTTGTTTGTAC 413 Query 462 TCGCTTCAAGGCTGAGTACCCCATATTTGATAAGGTTGACGTGAATGGTGATAATGCTGC 521 || |||||| | |||| ||| |||||||| ||| |||| ||||||||||| ||||| | Sbjct 414 ACGTTTCAAGTCAGAGTTCCCTATATTTGACAAGATTGAAGTGAATGGTGAAAATGCATC 473 Query 522 TCCACTGTATAAGTTCTTGAAATCAAGCAAAGGTGGGT-TCTTTGGAGATAGTATCAAGT 580 ||| || |||||||| |||||||| | |||| ||| | ||||| ||| ||| ||| Sbjct 474 TCCTCTTTATAAGTTGTTGAAATCGGG-AAAGTGGGGAATATTTGGGGATGATATTCAGT 532 Query 581 GGAACTTCTCCAAATTCCTTGTTGACAAAGAA-GGAAACGTCGTTGATCGCTACTCTCCA 639 |||| || | || ||||| ||||| || ||| ||| | | | ||||||||| | ||| Sbjct 533 GGAATTTTGCTAAGTTCCTGGTTGATAA-GAATGGACAGGCTGCTGATCGCTATTATCCT 591 Query 640 ACGACTGCTCCAGCTAGC-ATGGAGAAGGATATCAAGAAA 678 || || |||| || | || |||| |||||| || ||| Sbjct 592 ACCACCTCTCCT-CTGACAATTGAGAGGGATATAAA-AAA 629 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 54335154150 Database: N.benthamiana Genome v1.0.1 predicted cDNA Posted date: Mar 21, 2024 4:04 PM Number of letters in database: 82,582,885 Number of sequences in database: 57,140 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5