BLASTP 2.11.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: N.benthamiana Genome v1.0.1 predicted proteins 57,140 sequences; 18,711,326 total letters Query= Untitled_sequence Length=621 Score E Sequences producing significant alignments: (Bits) Value Niben101Scf11609g03002.1sp|Q43358|VCL_THECC *-*- Vicilin IPR00679... 300 7e-92 Niben101Scf09547g02001.1sp|Q43358|VCL_THECC *-*- Vicilin IPR00679... 295 2e-89 Niben101Scf11609g02002.1sp|Q43358|VCL_THECC ***- Vicilin IPR01471... 235 5e-71 Niben101Scf02829g09016.1sp|Q43358|VCL_THECC ***- Vicilin IPR01471... 233 3e-69 Niben101Scf05557g00002.1sp|Q43358|VCL_THECC ***- Vicilin IPR01471... 231 3e-68 Niben101Scf03072g05007.1sp|Q9SPL4|AMP22_MACIN *-*- Vicilin-like a... 91.3 6e-19 Niben101Scf03693g07011.1sp|Q9SPL4|AMP22_MACIN *-*- Vicilin-like a... 90.5 1e-18 Niben101Scf00482g10005.1ref|XP_003597166.1| ***- Vicilin-like ant... 86.7 1e-17 Niben101Scf04639g10006.1ref|XP_003597166.1| ***- Vicilin-like ant... 85.9 2e-17 Niben101Ctg15499g00003.1ref|XP_003597166.1| ***- Vicilin-like ant... 85.9 2e-17 >Niben101Scf11609g03002.1 sp|Q43358|VCL_THECC *-*- Vicilin IPR006792 (Vicilin, N-terminal), IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=777 Score = 300 bits (769), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 153/432 (35%), Positives = 270/432 (63%), Gaps = 32/432 (7%) Query 205 RHKNKNPFHFNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNSKPNTLLLP 264 R + NP+ F S+RF++ F+ +G RVLQ+F +RS+ L+ + +YR+ +P + +LP Sbjct 355 REEENNPYFFESQRFRSRFRATHGDFRVLQKFTERSELLRGIENYRVAVIEFEPLSFMLP 414 Query 265 HHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDNDENLRMIT 324 HH DA+ + V++ G +++ D+++S+NL+ GD +RV AG+T Y++N DN E + Sbjct 415 HHCDAEAIFVVVRGRGTISIAEQDEKNSFNLEHGDVIRVDAGSTIYMLNRDNSERFFVYV 474 Query 325 LAIPVNKPGRFESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFGREEGQQQGE 384 LA VN PG+F+ FF + Q +++ FS +ILE +++T + + + LFG+ Q+QG Sbjct 475 LAQAVNTPGQFQEFFSAGGQNPETFYGAFSSDILETAFNTPRDRLER-LFGQ---QKQG- 529 Query 385 ERLQESVIVEISKKQIRELSKHA-KSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITP 443 ++++ S++QIR +S+HA +S+ ++T PFNL P+ +NK GK FE +P Sbjct 530 ------IVIKASEEQIRAISEHASRSTKQQTKGQTTGPFNLLKERPLITNKFGKFFEASP 583 Query 444 EKNPQLRDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIEL----VGIKEQQQ- 498 ++ QLRDLD ++ V++N+G + LPH+N+++ + ++ EG E+ +G + Q+Q Sbjct 584 DRFEQLRDLDAAVAFVNINQGGMLLPHYNTRSTRLAMVVEGSGRFEMACPHLGSQSQRQG 643 Query 499 -----RQQQEEQPLEV--RKYRAELSEQDIFVIPAGYPVVVNAT--SDLNFFAFGINAEN 549 R+Q+ E+ +V ++ R LS D+ ++PAG+PV + A+ S+L FG+NA N Sbjct 644 SRGERREQESEEGGDVHYQRVRGTLSVGDVLIVPAGHPVTIVASGGSNLRIVGFGVNAHN 703 Query 550 NQRNFLAGSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSESYFVDAQPQQKEEGNKGR 609 N++NFLAG + N+ + + +EL+F K++E +++ Q +SYFV+ P+Q+ + +G Sbjct 704 NRKNFLAGQQ-NIWRNVDREAKELSFNMPGKEVEEILQRQDQSYFVEG-PEQRGQRERGE 761 Query 610 KGP----LSSIL 617 +G LSSIL Sbjct 762 EGKGQQYLSSIL 773 >Niben101Scf09547g02001.1 sp|Q43358|VCL_THECC *-*- Vicilin IPR006792 (Vicilin, N-terminal), IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=793 Score = 295 bits (754), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 158/444 (36%), Positives = 271/444 (61%), Gaps = 37/444 (8%) Query 197 SESQREPRRHKNKNPFHFNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNS 256 E + P+R + NP+ F S+RF++ F+ +G RVLQ+F +RS+ L+ + +YR+ Sbjct 360 GEERENPQREEENNPYLFESQRFRSRFRATHGDFRVLQKFTQRSELLRGIENYRVAVIEF 419 Query 257 KPNTLLLPHHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDN 316 +P + +LPHH DA+ + V++ G +++ D+++S+NL+ GD +RV AG+T Y++N DN Sbjct 420 EPMSFMLPHHCDAEAIFVVVRGRGTISIAEQDEKNSFNLEHGDVIRVNAGSTIYMLNRDN 479 Query 317 DENLRMITLAIPVNKPGRFESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFGR 376 +E + LA +N PG F+ FF + Q +S+ + FS +ILE +++T + + + LFG+ Sbjct 480 NERFFVYVLAQAMNTPGEFQEFFSAGGQNPESFYRAFSSDILETAFNTPRDRLER-LFGQ 538 Query 377 EEGQQQGEERLQESVIVEISKKQIRELSKHA-KSSSRKTISSEDKPFNLRSRDPIYSNKL 435 Q+QG ++++ S++QIR +S+HA +S+ ++T PFNL P+ S+K Sbjct 539 ---QKQG-------IVIKASEEQIRAISEHASRSTKQQTKGQATGPFNLLKERPLISSKF 588 Query 436 GKLFEITPEKNPQLRDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELVG--I 493 GK FE +P++ QLRDLD ++ +++N+G + LP++N+++ + ++ EG E+ + Sbjct 589 GKFFEASPDRFEQLRDLDAAVAFMNINQGGMVLPYYNTRSTRLAMVVEGTGRFEMACPHL 648 Query 494 KEQQQRQ-----------QQEEQPLEVRKYRAELSEQDIFVIPAGYPVVVNAT--SDLNF 540 Q QRQ ++E + +K R LS D+ V+PAG+P+ + AT S+L Sbjct 649 GSQSQRQGSRGERREQESEEEGGDVHYQKVRGTLSVGDVLVVPAGHPITIIATGGSNLRI 708 Query 541 FAFGINAENNQRNFLAGSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSESYFVDAQP- 599 FG+NA NN++NFLAG + N+ + + +EL+F K++E +++ Q +SYFV A P Sbjct 709 VGFGLNAHNNKKNFLAGQQ-NIWRNVDREAKELSFNMPGKEVEEILQRQDQSYFV-AGPE 766 Query 600 ----QQKEEGNKGRKGP--LSSIL 617 QQ+E G +G KG LSSIL Sbjct 767 QRGQQQRERGEEG-KGQQYLSSIL 789 >Niben101Scf11609g02002.1 sp|Q43358|VCL_THECC ***- Vicilin IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=414 Score = 235 bits (600), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 139/416 (33%), Positives = 233/416 (56%), Gaps = 45/416 (11%) Query 210 NPFHFNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNSKPNTLLLPHHADA 269 NP+ F S RF++ F +++G RVL++F +RS+ L L YR+ +P + +LPHH DA Sbjct 31 NPYLFKSHRFRSRFTSKHGEFRVLEKFTERSEFLGGLDKYRVAVLEFEPLSFMLPHHCDA 90 Query 270 DYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDNDENLRMITLAIPV 329 + + ++ G +++ ++++S+NL++GD +RV G+T Y++N DN+E L Sbjct 91 EVIFFVVRGRGTISIAEQEEKNSFNLENGDVIRVNVGSTIYMINRDNNERL--------- 141 Query 330 NKPGRFESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFGREEGQQQGEERLQE 389 FE +F + +S+ + FS ILE +++T + I ++ GQQ ++ Sbjct 142 -----FE-YFSGGGENPESFYRAFSSEILETAFNTPRDTIERLF-----GQQ------RK 184 Query 390 SVIVEISKKQIRELSKHAKSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITPEKNPQL 449 V V+ S++QI+ +S+HA SR PFNL P+ NK G FE TPE+ QL Sbjct 185 GVFVKASEEQIKAISEHA---SRSIKQQTRGPFNLLKERPLLGNKFGHYFEATPERFKQL 241 Query 450 RDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELVG--IKEQQQRQQQEEQPL 507 RDLD ++++N G + LP + ++ +++ EG E+ + Q QRQ + Q + Sbjct 242 RDLDAAAGIMNINRGGMLLPVYCTRTTWFVMVVEGNGRFEMACPHLGSQSQRQGRHYQKV 301 Query 508 EVRKYRAELSEQDIFVIPAGYPVVVNAT--SDLNFFAFGINAENNQRNFLAGSKDNVISQ 565 E LS D+ +I G+P+ V AT SDL G NA NN+ NFLAG + ++ Sbjct 302 E-----GSLSVGDVMIIREGHPITVIATGDSDLRMVGIGFNAHNNRMNFLAGGQ-SIWRN 355 Query 566 IPSQVQELAFPGSAKDIENLIKSQSESYFVDAQPQQK-----EEGNKGRKGPLSSI 616 I + +E++F +A ++E +++ Q +SYFV A+P+Q+ EEG +GR+ SS+ Sbjct 356 IDREAKEVSFNMAASEVEEILQKQDQSYFV-ARPEQRQQQMSEEGTRGRQYVSSSL 410 >Niben101Scf02829g09016.1 sp|Q43358|VCL_THECC ***- Vicilin IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=498 Score = 233 bits (595), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 136/413 (33%), Positives = 228/413 (55%), Gaps = 27/413 (7%) Query 210 NPFHFNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNSKPNTLLLPHHADA 269 NP+ F + F T K Q+G +RVL +F +RS+ + + +YR+ + P T +LP+H DA Sbjct 95 NPYVFEDQHFITGIKTQHGRIRVLPKFTERSKLHKGIENYRVAILEANPQTFILPNHWDA 154 Query 270 DYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDNDENLRMITLAIPV 329 D ++ + G L LV R+ +N++ GD +RV AGTT Y++N DN+E L + L P+ Sbjct 155 DAVLFVAQGRGTLNLVRRGKRNGFNIRPGDVIRVHAGTTAYLINRDNNEKLVIAKLLNPI 214 Query 330 NKPGRFESFFLSSTQAQ-QSYLQGFSKNILEASYDTKFEEINKVLFGREEGQQQGEERLQ 388 + PG+FE FF Q +S+ FS ILE++ + + + + + LFG++ + Sbjct 215 STPGQFEHFFGPGGQENPESFYNAFSSEILESALNVRRDRLQR-LFGQQ----------R 263 Query 389 ESVIVEISKKQIRELSKHAKSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITPEKNPQ 448 E VI+ S++QIR +S+H + NL + P+ SN+ G+L+E+ Q Sbjct 264 EGVIIRASEEQIRAMSQHEEGGIWPFGGESKGSVNLYRQRPLQSNQYGQLYEVDGSHYRQ 323 Query 449 LRDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELV--------GIKEQQQRQ 500 L DLD+ +S+ ++ +G + P ++S+A + V+ +GE +E+ G + +Q R Sbjct 324 LEDLDISVSLANITQGGMLGPAYSSRATKIAVVVDGEGYLEMACPHLSSESGSRGRQSRG 383 Query 501 QQEEQPL--EVRKYRAELSEQDIFVIPAGYPVVVNATS--DLNFFAFGINAENNQRNFLA 556 + E + +K RA L + +IPAG+PVV A++ +L F INA NN+ LA Sbjct 384 SERETRIGSGYQKVRARLRRGMVVIIPAGHPVVNVASNNQNLQVVCFNINASNNEIVPLA 443 Query 557 GSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSESYFVDAQP--QQKEEGNK 607 G ++NV+SQ+ + EL F A+++E + +SQ E F QQ +EG + Sbjct 444 G-RNNVMSQLEREAMELGFGVPAREVEQIFRSQQEELFFQGPGGRQQHQEGGR 495 >Niben101Scf05557g00002.1 sp|Q43358|VCL_THECC ***- Vicilin IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=497 Score = 231 bits (588), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 133/398 (33%), Positives = 222/398 (56%), Gaps = 25/398 (6%) Query 210 NPFHFNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNSKPNTLLLPHHADA 269 NP+ F + F T K Q+G +RVL +F +RS+ L+ + +YR+ + P ++P+H DA Sbjct 94 NPYVFEDQHFITGIKTQHGRIRVLPKFTERSKLLKGIENYRVAILEANPQAFIVPNHWDA 153 Query 270 DYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDNDENLRMITLAIPV 329 D ++ + G L LV R+S+N++ GD +RV AGTT Y++N DN+E L + L PV Sbjct 154 DAVLFVAQGRGTLNLVRRGKRNSFNIRPGDVIRVHAGTTAYLINRDNNEKLVIAKLLNPV 213 Query 330 NKPGRFESFFLSSTQAQ-QSYLQGFSKNILEASYDTKFEEINKVLFGREEGQQQGEERLQ 388 + PG+FE FF + +S+ FS ILE++ + + + + + LFG++ + Sbjct 214 STPGQFEHFFGPGGRDNLESFYNAFSSEILESALNVRRDRLQR-LFGQQ----------R 262 Query 389 ESVIVEISKKQIRELSKHAKSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITPEKNPQ 448 E VI+ S++QIR +S+H + NL + P+ SNK G+L+E+ Q Sbjct 263 EGVIIRASEEQIRAMSQHEEGGIWPFGGESKGSVNLYRQRPLQSNKYGQLYEVDGSHYRQ 322 Query 449 LRDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELV--------GIKEQQQRQ 500 L DLD+ +S+ ++ +G +F P ++S+ + V+ +GE E+ G + +Q R Sbjct 323 LEDLDISVSLANITQGGMFGPAYSSRVTKIAVVVDGEGYFEMACPHLVSESGGRGRQSRG 382 Query 501 QQEEQPL--EVRKYRAELSEQDIFVIPAGYPVVVNATS--DLNFFAFGINAENNQRNFLA 556 + E + +K RA L + +IPAG+PVV A++ +L F INA NN+ LA Sbjct 383 SERETRIGSGYQKVRARLRRGMVVIIPAGHPVVNVASNNQNLQVVCFNINARNNEIVPLA 442 Query 557 GSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSESYF 594 G + NV+SQ+ + EL F A+++E + +SQ E +F Sbjct 443 G-RSNVMSQLERETMELGFGVPAREVEEIFRSQQEEFF 479 >Niben101Scf03072g05007.1 sp|Q9SPL4|AMP22_MACIN *-*- Vicilin-like antimicrobial peptides 2-2 IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=692 Score = 91.3 bits (225), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 80/366 (22%), Positives = 164/366 (45%), Gaps = 36/366 (10%) Query 248 DYRILEFNSKPNTLLLPHHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGT 307 Y + +PN+L LP AD + + G+ LT V+ ++ S +L+ GD R+P GT Sbjct 82 SYHLQFITLEPNSLFLPVVLHADMVFYVHTGSGRLTWVDETEQKSVHLRIGDVFRLPCGT 141 Query 308 TYYVVNPDNDENLRMITLAIPVNKPGRFESFFLSST-----------QAQQSYLQGFSKN 356 +++ + NL P + R S F +S + + + GF + Sbjct 142 IFFI-----ESNLE------PARQKLRVYSIFANSGDDLREPLSGPYSSIRDMVLGFDRK 190 Query 357 ILEASYDTKFEEINKVLFGREE-GQQQGEERLQESVIVEISKKQIRELSKHAKSSSRKTI 415 +L+A++ + I++VL G E G + + + E+ + ++ L S + Sbjct 191 VLQAAFHVPEDVIDEVLNGTEVPAIIHGVPKTTKKTLWEMEAEFMKSLLARGGHSFFDSQ 250 Query 416 SSEDKP--FNLRSRDPIYSNKLGKLFEITPEKNPQLRDLDVFLSVVDMNEGALFLPHFNS 473 S++ K FN+ P + N G IT +K P L+ + VV++ +G++ PH+N Sbjct 251 SNKKKTELFNIFKEKPDFENCNGWSTVITRKKLPALKGSHTGIYVVNLTKGSMMAPHWNP 310 Query 474 KAIVVLVINEGEANIELVGIKEQQQRQQQEEQPLEVRKYRAELSEQDIFVIPAGYPVVVN 533 A + + +GE + +V + L + R + + D+F +P +P+ Sbjct 311 TATEIGIALQGEGMVRVVCSSTGTE--------LGCKNMRFNVEQGDVFAVPRFHPMAQM 362 Query 534 ATSDLNFFAFGINAENNQRN--FLAGSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSE 591 A ++ +F G + + + +L G K +V+ + Q+ +F + ++ ++++Q E Sbjct 363 AFNNNSFVFVGFSTTTGRHHPQYLTG-KASVLRTLDRQILAASFNVTNTTMDRILEAQGE 421 Query 592 SYFVDA 597 S ++ Sbjct 422 SVILEC 427 >Niben101Scf03693g07011.1 sp|Q9SPL4|AMP22_MACIN *-*- Vicilin-like antimicrobial peptides 2-2 IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=714 Score = 90.5 bits (223), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 82/373 (22%), Positives = 165/373 (44%), Gaps = 36/373 (10%) Query 248 DYRILEFNSKPNTLLLPHHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGT 307 Y + +PN+L LP AD + + G+ LT ++ + S +++ GD R+P T Sbjct 82 SYHLQFITLEPNSLFLPVVLHADMVFYVHTGSGRLTWMDETEHKSVDIRIGDVFRLPYET 141 Query 308 TYYVVNPDNDENLRMITLAIPVNKPGRFESFFLSSTQAQQSYLQG-----------FSKN 356 +++ + NL P + R S F +S + L G F +N Sbjct 142 IFFI-----ESNLE------PARQKLRVYSIFANSGNDLREPLSGPYSRIRDMVLGFDRN 190 Query 357 ILEASYDTKFEEINKVLFGREE-GQQQGEERLQESVIVEISKKQIRELSKHAKSSSRKTI 415 +L+A++ + I++VL G E G + + + E+ + ++ L S + Sbjct 191 VLQAAFHVPEDVIDEVLNGTEAPAIIHGVPKTTKKTLWEMEAQFMKSLLGRGGHSFFDSQ 250 Query 416 SSEDKP--FNLRSRDPIYSNKLGKLFEITPEKNPQLRDLDVFLSVVDMNEGALFLPHFNS 473 S++ K FN+ P + N G IT +K P L+ + + VV++ +G++ PH+N Sbjct 251 SNKKKTELFNIFKEKPDFENCNGWSTVITRKKLPALKGSHIGIYVVNLTKGSMMGPHWNP 310 Query 474 KAIVVLVINEGEANIELVGIKEQQQRQQQEEQPLEVRKYRAELSEQDIFVIPAGYPVVVN 533 A + + +GE + +V Q+ + R + E D+F +P +P+ Sbjct 311 TATEIGIALQGEGMVRVVCSSTGTQQG--------CKNMRFNVEEGDVFAVPRFHPMAQM 362 Query 534 ATSD--LNFFAFGINAENNQRNFLAGSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSE 591 A ++ F F + + +L G K +V+ + Q+ +F + + I+ ++++Q E Sbjct 363 AFNNNSFVFVGFSTTTKRHHPQYLTG-KASVLRTLDRQILAASFNVTNRTIDRILEAQGE 421 Query 592 SYFVDAQPQQKEE 604 + ++ +EE Sbjct 422 AVILECTSCAEEE 434 >Niben101Scf00482g10005.1 ref|XP_003597166.1| ***- Vicilin-like antimicrobial peptides 2-3 [Medicago truncatula] IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=486 Score = 86.7 bits (213), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 89/415 (21%), Positives = 173/415 (42%), Gaps = 53/415 (13%) Query 214 FNSKRFQTLFKNQYGHVRVLQRFNKRSQQLQNLRDYRILEFNSKPNTLLLPHHADADYLI 273 F ++ + K + G +++++ +R + I + +PN+L +P + D++ L+ Sbjct 45 FLLRQLHDVVKTEAGAMKLVKGGYRRGTSFHS--PMHIGFISMEPNSLFIPQYLDSNLLL 102 Query 274 VILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDNDENLRMI-TLAIPVNKP 332 + +G A + + D+ NL+ GD VPAG+ +Y+ N ++ L +I ++ + Sbjct 103 FVHHGEARVGHMYKDELAERNLKPGDVYTVPAGSAFYLENIVKNQRLHVICSIDVTSESM 162 Query 333 G--RFESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFGREEG-----QQQGEE 385 G F+SFF+ S L GF L + + EE++ L + G Sbjct 163 GWHAFQSFFIGGGNYPTSILAGFDHTTLSTALNVSTEELSTFLTRQSSGPIVHLSGSHGA 222 Query 386 RLQESVIVEISKKQIRELSKHA-------------KSSSRKTISS----ED--------- 419 + + + K++ L + A S RK + + ED Sbjct 223 HIWSKFLAQEPHKRLAHLKRIANFEEETIPKEEESTWSLRKFLFTLLNREDVVKRVNHKA 282 Query 420 -KPFNLRSRDPIYSNKLGKLFEITPEKNPQLRDLDVFLSVVDMNEGALFLPHFNSKAIVV 478 +NL +R + N G ++ L+ + +V+++ G++ PH N AI Sbjct 283 PSAYNLYNRKADFKNDYGWSKKLDESNYSPLKISGNGVYLVNLSRGSMMAPHVNPTAIEY 342 Query 479 LVINEGEANIELVGIKEQQQRQQQEEQPLEVRKYRAELSEQDIFVIPAGYPVVVNATSD- 537 ++ +G I++V P A + E D+F +P +P A+++ Sbjct 343 GIVLKGTGRIQIV-------------YPNGTLAMNARVREGDVFWVPRYFPFCQIASTNG 389 Query 538 -LNFFAFGINAENNQRNFLAGSKDNVISQIPSQVQELAFPGSAKDIENLIKSQSE 591 FF F +A N + FL G KD+++ + AF S K ++ + +QSE Sbjct 390 PFEFFGFTTSAGRNHQQFLVG-KDSLMQSLKGPEFAAAFGISEKRLKRIANAQSE 443 >Niben101Scf04639g10006.1 ref|XP_003597166.1| ***- Vicilin-like antimicrobial peptides 2-3 [Medicago truncatula] IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=510 Score = 85.9 bits (211), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 84/388 (22%), Positives = 151/388 (39%), Gaps = 65/388 (17%) Query 258 PNTLLLPHHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDND 317 PNTL +P + D+ + + G + + D+ L+ GD + AG+ +Y+VNP Sbjct 89 PNTLFIPQYLDSHLTLFVRRGETRIGHIYKDEFVERRLKEGDVYSIRAGSAFYLVNPTEG 148 Query 318 ENLRMITLAIPVNKPGR--FESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFG 375 + L +I G F+SFF+ S L GF L +++ E++++L Sbjct 149 QRLHIICSISTSTSLGWHGFQSFFIGGGMYPTSVLAGFDSLTLSTAFNVSTTEVSEILTR 208 Query 376 REEG-----------------------QQQGEERLQESVIVEISKKQ---------IREL 403 + G Q Q E L+ V +E Q I L Sbjct 209 QYSGAIVYLNTTHSPTPSIWATFLNLEQHQKLEHLKRIVHLEAKASQEEDEEKEQPIWSL 268 Query 404 SKHA--------------KSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITPEKNPQL 449 K K R+T D +NL R Y N G + L Sbjct 269 RKFLTNLIGNERNRREGKKGDDRRTGKGPDS-YNLFDRKADYKNDYGWSLALDQSDYSPL 327 Query 450 RDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELVGIKEQQQRQQQEEQPLEV 509 + D+ + +V+++ GA+ PH N A ++ G +I++V P Sbjct 328 KHADIGVYLVNLSAGAMMAPHINPTATEYGIVLRGSGSIQIV-------------YPNGT 374 Query 510 RKYRAELSEQDIFVIPAGYPV--VVNATSDLNFFAFGINAENNQRNFLAGSKDNVISQIP 567 + ++E D+F +P +P + + T FF F A+ N+ FL G +++++ + Sbjct 375 LAMNSRVNEGDVFWVPRYFPFCQIASRTGPFEFFGFTTTAKKNRPQFLVG-QNSILQSMR 433 Query 568 SQVQELAFPGSAKDIENLIKSQSESYFV 595 AF S + ++ ++ +Q E+ + Sbjct 434 GPEFAAAFGVSEERLKRILDAQREAVIL 461 >Niben101Ctg15499g00003.1 ref|XP_003597166.1| ***- Vicilin-like antimicrobial peptides 2-3 [Medicago truncatula] IPR014710 (RmlC-like jelly roll fold) GO:0045735 (nutrient reservoir activity) Length=510 Score = 85.9 bits (211), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 84/388 (22%), Positives = 151/388 (39%), Gaps = 65/388 (17%) Query 258 PNTLLLPHHADADYLIVILNGTAILTLVNNDDRDSYNLQSGDALRVPAGTTYYVVNPDND 317 PNTL +P + D+ + + G + + D+ L+ GD + AG+ +Y+VNP Sbjct 89 PNTLFIPQYLDSHLTLFVRRGETRIGHIYKDEFVERRLKEGDVYSIRAGSAFYLVNPTEG 148 Query 318 ENLRMITLAIPVNKPGR--FESFFLSSTQAQQSYLQGFSKNILEASYDTKFEEINKVLFG 375 + L +I G F+SFF+ S L GF L +++ E++++L Sbjct 149 QRLHIICSISTSTSLGWHGFQSFFIGGGMYPTSVLAGFDSLTLSTAFNVSTTEVSEILTR 208 Query 376 REEG-----------------------QQQGEERLQESVIVEISKKQ---------IREL 403 + G Q Q E L+ V +E Q I L Sbjct 209 QYSGAIVYLNTTHSPTPSIWATFLNLEQHQKLEHLKRIVHLEAKASQEEDEEKEQPIWSL 268 Query 404 SKHA--------------KSSSRKTISSEDKPFNLRSRDPIYSNKLGKLFEITPEKNPQL 449 K K R+T D +NL R Y N G + L Sbjct 269 RKFLTNLIGNERNRREGKKGDDRRTGKGPDS-YNLFDRKADYKNDYGWSLALDQSDYSPL 327 Query 450 RDLDVFLSVVDMNEGALFLPHFNSKAIVVLVINEGEANIELVGIKEQQQRQQQEEQPLEV 509 + D+ + +V+++ GA+ PH N A ++ G +I++V P Sbjct 328 KHADIGVYLVNLSAGAMMAPHINPTATEYGIVLRGSGSIQIV-------------YPNGT 374 Query 510 RKYRAELSEQDIFVIPAGYPV--VVNATSDLNFFAFGINAENNQRNFLAGSKDNVISQIP 567 + ++E D+F +P +P + + T FF F A+ N+ FL G +++++ + Sbjct 375 LAMNSRVNEGDVFWVPRYFPFCQIASRTGPFEFFGFTTTAKKNRPQFLVG-QNSILQSMR 433 Query 568 SQVQELAFPGSAKDIENLIKSQSESYFV 595 AF S + ++ ++ +Q E+ + Sbjct 434 GPEFAAAFGVSEERLKRILDAQREAVIL 461 Lambda K H a alpha 0.312 0.131 0.371 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 6475035844 Database: N.benthamiana Genome v1.0.1 predicted proteins Posted date: Mar 21, 2024 4:04 PM Number of letters in database: 18,711,326 Number of sequences in database: 57,140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40