BLASTN 2.11.0+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: Nitab v4.5 cDNA Edwards2017 69,500 sequences; 76,611,276 total letters Query= Untitled_sequence Length=733 Score E Sequences producing significant alignments: (Bits) Value Nitab4.5_0000906g0010.1DnaJ domain, HSP40/DnaJ peptide-binding, D... 1002 0.0 Nitab4.5_0001301g0160.1HSP40/DnaJ peptide-binding, DnaJ domain, C... 946 0.0 Nitab4.5_0011161g0010.1DnaJ domain, HSP40/DnaJ peptide-binding, C... 654 0.0 Nitab4.5_0010861g0010.1DnaJ domain, Chaperone DnaJ, C-terminal, H... 654 0.0 Nitab4.5_0002899g0020.1 126 4e-28 Nitab4.5_0002693g0050.1 110 4e-23 >Nitab4.5_0000906g0010.1 DnaJ domain, HSP40/DnaJ peptide-binding, DnaJ domain, conserved site, Chaperone DnaJ, C-terminal Length=1041 Score = 1002 bits (542), Expect = 0.0 Identities = 563/573 (98%), Gaps = 1/573 (0%) Strand=Plus/Plus Query 25 GTTTGGAGGGAGAAAAACATATTAAAGCCGGCACCGGGTAAGAGGCAGTGCAACTGCCGA 84 |||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| Sbjct 469 GTTTGGAGGGAGAAAAACATATTAAAGCCGGCACCGGGCAAGAGGCAGTGCAACTGCCGA 528 Query 85 AACGAAGTATATCATCGACAAATCGGTCCAGGAATGTTTCATCAGATGACTGAGCAGGTC 144 ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| Sbjct 529 AACGAAGTATATCATCGACAAATCGGTCCAGGAATGTTTCAGCAGATGACTGAGCAGGTC 588 Query 145 TGTGACCAATGCCCCAACGTGAA-CTTGAAAGGGAGGGCTACCACATTACTGTGGATATT 203 ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| Sbjct 589 TGTGACCAATGCCCCAACGTGAAGTTTGAAAGGGAGGGCTACCACATTACTGTGGATATT 648 Query 204 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGCCTTTTATGAGGATGCTGAGCCTAAGATA 263 ||||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||| Sbjct 649 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGTCTTTTATGAGGATGGTGAGCCTAAGATA 708 Query 264 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 323 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 709 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 768 Query 324 AGAGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGT 383 ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||| Sbjct 769 AGAGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGTGCAAGCTCTGGTTGGT 828 Query 384 TTTGAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTACCTCAAAGGGAATC 443 |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||| Sbjct 829 TTTGAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTAGTTCAAAGGGAATC 888 Query 444 ACTAAACCGAAGGAAGTGAGAAAGTTTAAAGGCAAAGGGATGCCACTGCATTTTAGTAAC 503 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 889 ACTAAACCGAAGGAAGTGAGAAAGTTTAAAGGCAAAGGGATGCCACTGCATTTTAGTAAC 948 Query 504 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 563 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 949 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 1008 Query 564 CACAAGAAACAGATCAAGGAAATTCTTGGCTAA 596 || |||||||||||||||||||||||||||||| Sbjct 1009 CAGAAGAAACAGATCAAGGAAATTCTTGGCTAA 1041 >Nitab4.5_0001301g0160.1 HSP40/DnaJ peptide-binding, DnaJ domain, Chaperone DnaJ, C-terminal, DnaJ domain, conserved site Length=1044 Score = 946 bits (512), Expect = 0.0 Identities = 553/573 (97%), Gaps = 1/573 (0%) Strand=Plus/Plus Query 25 GTTTGGAGGGAGAAAAACATATTAAAGCCGGCACCGGGTAAGAGGCAGTGCAACTGCCGA 84 |||||||||||||||||||| ||||| ||||||||||||||||||| ||||||||||||| Sbjct 472 GTTTGGAGGGAGAAAAACATTTTAAAACCGGCACCGGGTAAGAGGCGGTGCAACTGCCGA 531 Query 85 AACGAAGTATATCATCGACAAATCGGTCCAGGAATGTTTCATCAGATGACTGAGCAGGTC 144 || || ||||||||||||||||||||||||||||||||||| |||||||||||||||||| Sbjct 532 AATGAGGTATATCATCGACAAATCGGTCCAGGAATGTTTCAGCAGATGACTGAGCAGGTC 591 Query 145 TGTGACCAATGCCCCAACGTGAA-CTTGAAAGGGAGGGCTACCACATTACTGTGGATATT 203 ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| Sbjct 592 TGTGACCAATGCCCCAACGTGAAGTTTGAAAGGGAGGGCTACCACATTACTGTGGATATT 651 Query 204 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGCCTTTTATGAGGATGCTGAGCCTAAGATA 263 ||||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||| Sbjct 652 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGTCTTTTATGAGGATGGTGAGCCTAAGATA 711 Query 264 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 323 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 712 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 771 Query 324 AGAGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGT 383 || |||||||||||||||||||||||||||||||||||||||| |||||||||||||||| Sbjct 772 AGGGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGTGCAAGCTCTGGTTGGT 831 Query 384 TTTGAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTACCTCAAAGGGAATC 443 ||||||||||||||||||||||||||||||||||| |||||||||| |||||||||||| Sbjct 832 TTTGAGAAGACCATTAAACATCTCGATGATCATCTAGTGGATATTAGTTCAAAGGGAATC 891 Query 444 ACTAAACCGAAGGAAGTGAGAAAGTTTAAAGGCAAAGGGATGCCACTGCATTTTAGTAAC 503 |||||||| |||||||||||||||||||||||| ||||||||||||||||||||||| || Sbjct 892 ACTAAACCAAAGGAAGTGAGAAAGTTTAAAGGCGAAGGGATGCCACTGCATTTTAGTGAC 951 Query 504 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 563 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 952 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 1011 Query 564 CACAAGAAACAGATCAAGGAAATTCTTGGCTAA 596 || |||||||||||||||||||||||||| ||| Sbjct 1012 CAGAAGAAACAGATCAAGGAAATTCTTGGTTAA 1044 >Nitab4.5_0011161g0010.1 DnaJ domain, HSP40/DnaJ peptide-binding, Chaperone DnaJ, C-terminal, DnaJ domain, conserved site Length=1465 Score = 654 bits (354), Expect = 0.0 Identities = 497/568 (88%), Gaps = 1/568 (0%) Strand=Plus/Plus Query 25 GTTTGGAGGGAGAAAAACATATTAAAGCCGGCACCGGGTAAGAGGCAGTGCAACTGCCGA 84 |||||||| ||||| ||||| |||||||| || || |||||||||| ||||||||||| Sbjct 460 GTTTGGAGAGAGAAGAACATTTTAAAGCCAGCCCCCGGTAAGAGGCGCTGCAACTGCCGC 519 Query 85 AACGAAGTATATCATCGACAAATCGGTCCAGGAATGTTTCATCAGATGACTGAGCAGGTC 144 || || || ||||| || ||||| ||||| |||||||| || |||||||| ||||||||| Sbjct 520 AATGAGGTGTATCACCGGCAAATAGGTCCGGGAATGTTCCAACAGATGACAGAGCAGGTC 579 Query 145 TGTGACCAATGCCCCAACGTGAACTT-GAAAGGGAGGGCTACCACATTACTGTGGATATT 203 |||||| |||||||||| ||||| || |||||||||||||| | ||||||||||||||| Sbjct 580 TGTGACAAATGCCCCAATGTGAAGTTCGAAAGGGAGGGCTATGAAATTACTGTGGATATT 639 Query 204 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGCCTTTTATGAGGATGCTGAGCCTAAGATA 263 |||||||||||||||||||| || ||||| | ||||||||||||| ||||||||||||| Sbjct 640 GAGAAAGGAATGCAAGATGGACAAGAAGTCGTATTTTATGAGGATGGTGAGCCTAAGATA 699 Query 264 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 323 |||||||||||||||||||| ||||||||||| ||||||||||||||||||| ||||| Sbjct 700 GATGGTGAACCTGGAGACCTTAAGTTCCGCATTCGCACAGCTCCTCATGACCGCTTCAAG 759 Query 324 AGAGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGT 383 || |||||||| ||||| | ||| ||||||||||| || |||| ||||| ||||||||| Sbjct 760 AGGGAAGGAAATGACCTACACACAACTGTTACTATTACCCTGGTACAAGCGCTGGTTGGT 819 Query 384 TTTGAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTACCTCAAAGGGAATC 443 || || ||||||||||||||||| ||||| ||| | || |||||| | | ||||| ||| Sbjct 820 TTCGAAAAGACCATTAAACATCTTGATGACCATTTAGTAGATATTGGCACGAAGGGGATC 879 Query 444 ACTAAACCGAAGGAAGTGAGAAAGTTTAAAGGCAAAGGGATGCCACTGCATTTTAGTAAC 503 ||||| || |||||||| |||||| |||||||||||||||||||||||||||| ||| || Sbjct 880 ACTAAGCCAAAGGAAGTTAGAAAGATTAAAGGCAAAGGGATGCCACTGCATTTCAGTGAC 939 Query 504 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 563 ||||| || ||||| ||||||||||||||||||||||||||||||||||| || |||||| Sbjct 940 AAGAAAGGCGATTTGTATGTTAAATTTGAGGTTCTTTTCCCCACATCATTAACAGAGGAC 999 Query 564 CACAAGAAACAGATCAAGGAAATTCTTG 591 || ||||||||||||||||||||||||| Sbjct 1000 CAGAAGAAACAGATCAAGGAAATTCTTG 1027 >Nitab4.5_0010861g0010.1 DnaJ domain, Chaperone DnaJ, C-terminal, HSP40/DnaJ peptide-binding, DnaJ domain, conserved site Length=1466 Score = 654 bits (354), Expect = 0.0 Identities = 497/568 (88%), Gaps = 1/568 (0%) Strand=Plus/Plus Query 25 GTTTGGAGGGAGAAAAACATATTAAAGCCGGCACCGGGTAAGAGGCAGTGCAACTGCCGA 84 || ||||| ||||||||| | |||||||| || || |||||||||| ||||||||||| Sbjct 460 GTGTGGAGAGAGAAAAACGTTTTAAAGCCAGCCCCTGGTAAGAGGCGTTGCAACTGCCGC 519 Query 85 AACGAAGTATATCATCGACAAATCGGTCCAGGAATGTTTCATCAGATGACTGAGCAGGTC 144 || || || ||||| |||||||||||||| |||||||| || |||||||| ||||||||| Sbjct 520 AATGAGGTGTATCACCGACAAATCGGTCCGGGAATGTTCCAGCAGATGACAGAGCAGGTC 579 Query 145 TGTGACCAATGCCCCAACGTGAACTT-GAAAGGGAGGGCTACCACATTACTGTGGATATT 203 |||||| |||||||||| ||||| || |||||||||||||| | |||||||| ||||| Sbjct 580 TGTGACAAATGCCCCAATGTGAAGTTCGAAAGGGAGGGCTATGAAATTACTGTAGATATC 639 Query 204 GAGAAAGGAATGCAAGATGGTCAGGAAGTGGCCTTTTATGAGGATGCTGAGCCTAAGATA 263 ||||||||||||||||| || || ||||| | ||||||||||||| ||||||||||||| Sbjct 640 GAGAAAGGAATGCAAGACGGACAAGAAGTAGTATTTTATGAGGATGGTGAGCCTAAGATA 699 Query 264 GATGGTGAACCTGGAGACCTGAAGTTCCGCATCCGCACAGCTCCTCATGACCAGTTCAAA 323 ||||||||||| |||||| ||||||||||||| ||||||||||||||||||| ||||| Sbjct 700 GATGGTGAACCCGGAGACTTGAAGTTCCGCATTCGCACAGCTCCTCATGACCGCTTCAAG 759 Query 324 AGAGAAGGAAACGACCTTCGCACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGT 383 || |||||||| ||||| | ||| ||||||||||| ||||||| |||||| ||||||||| Sbjct 760 AGGGAAGGAAATGACCTACACACAACTGTTACTATTACTCTGGTGCAAGCGCTGGTTGGT 819 Query 384 TTTGAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTACCTCAAAGGGAATC 443 || || ||||||||||||||||||||||| ||| | || |||||| | ||||||| ||| Sbjct 820 TTCGAAAAGACCATTAAACATCTCGATGACCATTTAGTAGATATTGGCACAAAGGGTATC 879 Query 444 ACTAAACCGAAGGAAGTGAGAAAGTTTAAAGGCAAAGGGATGCCACTGCATTTTAGTAAC 503 ||||| || ||||||||||||||| ||||||| ||||||||||||||||||| |||||| Sbjct 880 ACTAAGCCAAAGGAAGTGAGAAAGATTAAAGGTGAAGGGATGCCACTGCATTTCAGTAAC 939 Query 504 AAGAAGGGTGATTTATATGTTAAATTTGAGGTTCTTTTCCCCACATCATTGACGGAGGAC 563 ||||| || |||||||||||||| |||||||||||||||||||||||||| || |||||| Sbjct 940 AAGAAAGGCGATTTATATGTTAATTTTGAGGTTCTTTTCCCCACATCATTAACAGAGGAC 999 Query 564 CACAAGAAACAGATCAAGGAAATTCTTG 591 || ||||||||||||||| ||||||||| Sbjct 1000 CAGAAGAAACAGATCAAGCAAATTCTTG 1027 >Nitab4.5_0002899g0020.1 Length=1248 Score = 126 bits (68), Expect = 4e-28 Identities = 181/236 (77%), Gaps = 6/236 (3%) Strand=Plus/Plus Query 210 GGAATGCAAGATGGTCAGGAAGTGGCCTTTTATGAGGATGCTGAGCCTAAGATAGATGGT 269 |||||||| ||||| ||||||| || | || ||||| |||||||| ||||||||| Sbjct 616 GGAATGCATGATGGGATGGAAGTGAGTTTGTGTGCGGATGGTGAGCCTACAATAGATGGT 675 Query 270 GAACCTGGAGACCTGAAGTTCCGCATC-C-GCACAGCTCCTCATGACCAGTTCAAAAGAG 327 || | |||||| || ||||||| ||| | | ||| || |||| || ||| | || || Sbjct 676 GAGCTTGGAGAACTAAAGTTCC--ATCTCAGTACAACTTTTCATTACTGGTTTACAACAG 733 Query 328 AAGGAAACGACCTTC-GCACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGTTTT 386 ||| ||| ||||||| || |||| || |||| | || ||||||||| | ||| ||| Sbjct 734 AAGAAAATGACCTTCATCA-CACTATTGCTATGAAGAGGGTGCAAGCTCTTGCTGGGTTT 792 Query 387 GAGAAGACCATTAAACATCTCGATGATCATCTGGTGGATATTACCTCAAAGGGAAT 442 |||||||||||||||||| | |||| ||||||||| |||| | ||||||||||| Sbjct 793 GAGAAGACCATTAAACATTTGGATGTTCATCTGGTAAATATGAGTTCAAAGGGAAT 848 >Nitab4.5_0002693g0050.1 Length=645 Score = 110 bits (59), Expect = 4e-23 Identities = 251/345 (73%), Gaps = 7/345 (2%) Strand=Plus/Plus Query 109 GGTCCAGGAATGTTTCATCAGATGACTGAGCAGGTCTGTGACCAATGCCCCAACGTGAAC 168 |||| |||||| |||| || |||| |||||| | || | ||||| ||||| | || Sbjct 106 GGTCAAGGAATAATTCAGCAAATGATTGAGCACATTTGCTATCAATGGCCCAATGCGAGG 165 Query 169 TT-GAAAGGGAGGGCTACCACATTACTGTGGATATTGAGAAAGGAATGCAAGATGGTCAG 227 || ||||| | |||| | || || ||||||| |||||||| ||||||| ||| | | Sbjct 166 TTCGAAAGCAAAGGCTTTCTCAGTATTGTGGATTTTGAGAAAAGAATGCATGATAGGATG 225 Query 228 GAAGTGGCCTTTTATGAGGA-TGCTGAGCCTAAGATAGATGGTGAACCTGGAGACCTGAA 286 || || | || | || || ||| ||||||||||||||| |||| | || ||| || || Sbjct 226 AAAATGACTTTGTGTGCAGATTGC-GAGCCTAAGATAGATAGTGAGCTTGAAGAACTAAA 284 Query 287 GTTCCGCATC-C-GCACAGCTCCTCATGACCAGTTCAAAAGAGAAGGAAACGACCTTCGC 344 ||| ||| | | ||| ||| ||||||| ||| |||||||||||| ||||||| | Sbjct 285 ATTCT--ATCTCTGTACATCTCTTCATGACTGGTTTGCAAGAGAAGGAAATGACCTTCCC 342 Query 345 ACCACTGTTACTATAACTCTGGCGCAAGCTCTGGTTGGTTTTGAGAAGACCATTAAACAT 404 ||| | ||||||| |||| ||||||||| | || | || || || ||||||||| Sbjct 343 GACACCATCACTATAAAGCTGGTGCAAGCTCTTGCGGGGTGTGTGACGATAATTAAACAT 402 Query 405 CTCGATGATCATCTGGTGGATATTACCTCAAAGGGAATCACTAAA 449 | |||| ||||||||| |||| | || ||||| ||| |||| Sbjct 403 TTGGATGTTCATCTGGTATATATGAGTTCGGAGGGACTCATTAAA 447 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 52886623132 Database: Nitab v4.5 cDNA Edwards2017 Posted date: Mar 21, 2024 4:12 PM Number of letters in database: 76,611,276 Number of sequences in database: 69,500 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5