Clone hba-1m12

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Clone
Clone name
hba-1m12
LE_HBa0001M12
C01HBa0001M12
P001M12
Clone type
BAC
Contamination
none
Estimated length
140,000
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2188 : [coherent], 7 members:
C01HBa0001M12, LE_HBa0122A12, C02HBa0189G15, LE_HBa0297F02, LE_HBa0310L17, LE_HBa0332H04, LE_HBa0336N23
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
cLET-14-E21Tomato-EXPEN 2000plate 8H1
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 1
FISH map 
View on FISH map chromosome 2
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
317-02 1CompositeFluorescencePhase77.3%
317-03 1CompositeFluorescencePhase76.3%
317-04 1CompositeFluorescencePhase78.3%
317-06 1CompositeFluorescencePhase77.3%
317-11 1CompositeFluorescencePhase77.2%
317-12 1CompositeFluorescencePhase76.2%
317-14 1CompositeFluorescencePhase78.3%
317-18 1CompositeFluorescencePhase78.4%
317-19 1CompositeFluorescencePhase78.8%
317-20 1CompositeFluorescencePhase78.1%
317-21 1CompositeFluorescencePhase76.1%
317-22 1CompositeFluorescencePhase76.6%
317-23 1CompositeFluorescencePhase77.1%
317-24 1CompositeFluorescencePhase77.1%
317-27 1CompositeFluorescencePhase77.0%
Mean±sd77.3%±0.9%

Given an estimated length of 18.9µm for chromosome 2Q and the mean percentage above, this BAC is approximately 14.6µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
USA
IL-mapped to chromosome
-
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
1 - fragmented, unordered
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
142,092 bp
C01HBa0001M12.2
AC218052.1 (GenBank) 		[View]
End Sequences
Warnings
  • sequence is not finished to HTGS3
 FTP
Chromosome 1 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (35)
1037, 1119, 1330, 1330, 1543, 1675, 2081, 2081, 2305, 2363, 2456, 2456, 2545, 2545, 2709, 2750, 2750, 2929, 2929, 3230, 3230, 3631, 3866, 4610, 4709, 5620, 6073, 6216, 6497, 6804, 8000, 8503, 8922, 9578, 9578
HindIII in silico (36)
1028, 1089, 1313, 1533, 1642, 2035, 2055, 2286, 2353, 2413, 2458, 2474, 2498, 2545, 2552, 2576, 2699, 2757, 2923, 2937, 3239, 3255, 3678, 3760, 3907, 4148, 4543, 4622, 4854, 5269, 6014, 6393, 7974, 8883, 9330, 9570
Fragment Lengths Match Score
0.97
Annotations  
Browse
LE_HBa0001M12 has no browsable sequence annotations
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End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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