Clone hba-300e15

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Clone
Clone name
hba-300e15
LE_HBa0300E15
C09HBa0300E15
P300E15
Clone type
BAC
Contamination
none
Estimated length
128,777
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2556 : [coherent], 1 members:
C09HBa0300E15
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1177Tomato-EXPEN 2000plate 15C9
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 9
FISH map 
View on FISH map chromosome 9
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
364-04 1CompositeFluorescencePhase44.5%
364-07 1CompositeFluorescencePhase48.3%
364-10 1CompositeFluorescencePhase42.8%
364-11 1CompositeFluorescencePhase49.8%
364-14 1CompositeFluorescencePhase52.0%
364-16 1CompositeFluorescencePhase43.8%
364-18 1CompositeFluorescencePhase44.3%
364-20 1CompositeFluorescencePhase45.1%
364-21 1CompositeFluorescencePhase43.7%
364-22a 1CompositeFluorescencePhase47.9%
364-22b 1CompositeFluorescencePhase48.7%
364-23 1CompositeFluorescencePhase51.2%
364-24 1CompositeFluorescencePhase46.5%
364-25 1CompositeFluorescencePhase52.5%
364-26 1CompositeFluorescencePhase48.7%
364-28 1CompositeFluorescencePhase52.2%
364-29 1CompositeFluorescencePhase47.4%
364-32 1CompositeFluorescencePhase45.2%
Mean±sd47.5%±3.2%

Given an estimated length of 5.4µm for chromosome 9P and the mean percentage above, this BAC is approximately 2.6µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 9 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 9
HTGS phase
2 - fragmented, ordered
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
122,248 bp
C09HBa0300E15.2
EF647596.1 (GenBank) 		[View]
End Sequences
None
Warnings
  • sequence is not finished to HTGS3
 FTP
Chromosome 9 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (28)
1131, 1139, 1458, 1619, 1844, 2006, 2006, 2228, 2633, 2680, 3156, 3177, 3328, 3362, 3571, 3595, 4124, 4231, 4391, 4435, 6338, 6589, 7086, 8215, 8779, 10965, 10965, 13726
HindIII in silico (27)
1052, 1143, 1439, 1475, 1610, 1824, 1862, 1958, 1990, 1994, 2230, 2649, 2714, 3187, 3360, 3621, 4077, 4241, 4419, 6132, 6509, 7059, 8034, 8675, 10823, 11000, 13250
Fragment Lengths Match Score
0.97
Annotations  
Browse
LE_HBa0300E15 has no browsable sequence annotations
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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