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Clone hba-76i13

Clone and library  
Clone
Clone name
hba-76i13
LE_HBa0076I13
C00HBa0076I13
P076I13
Clone type
BAC
Contamination
none
Estimated length
120,428
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
820-02a 1CompositeFluorescencePhase42.1%
820-02b 1CompositeFluorescencePhase36.8%
820-07 1CompositeFluorescencePhase32.5%
820-12 1CompositeFluorescencePhase42.8%
820-21 1CompositeFluorescencePhase36.6%
820-22 1CompositeFluorescencePhase40.7%
820-23 1CompositeFluorescencePhase36.7%
820-26 1CompositeFluorescencePhase34.5%
820-28 1CompositeFluorescencePhase35.3%
820-32 1CompositeFluorescencePhase38.8%
820-34 1CompositeFluorescencePhase39.1%
820-40 1CompositeFluorescencePhase39.3%
820-46 1CompositeFluorescencePhase40.1%
820-59 1CompositeFluorescencePhase41.2%
Mean±sd38.3%±3.0%

Given an estimated length of 11.7µm for chromosome 8Q and the mean percentage above, this BAC is approximately 4.5µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL Mapping 
Assigned to project
Italy
IL-mapped to chromosome
-
IL-mapped to IL segment
-
IL mapping notes
-
Sequencing  
This clone is registered to be sequenced, but has not been successfully mapped to any chromosome.

Log in as a curator or sequencer to edit this clone's registry information.
Sequencing Project
Chromosome unmapped
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Wellcome Trust Sanger Institute
Full Sequence
133,977 bp
C00HBa0076I13.1
CU457765.7 (GenBank)
[View]
[Download fasta]
[Download full submission] (0.08 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (26)
1146, 1593, 1724, 1990, 1990, 2077, 2097, 2157, 2247, 2573, 2573, 3210, 3865, 4099, 4099, 4322, 5688, 5688, 6257, 6599, 7405, 8223, 8634, 8634, 9589, 11949
HindIII in silico (33)
1023, 1121, 1127, 1138, 1309, 1500, 1603, 1723, 1949, 1980, 1989, 2074, 2111, 2163, 2242, 2569, 2593, 3208, 3906, 3951, 4110, 4110, 4312, 5334, 5388, 6090, 6539, 7303, 8066, 8539, 8547, 9405, 11801
Fragment Lengths Match Score
0.88
Annotations  
Browse
LE_HBa0076I13 has no browsable sequence annotations
Download
 
User comments [Add comment] 
Posted by Helen Beasley (sequencer) on 2008-04-10 04:54:56   
BAC sequenced by Chromosome 4 sequencing project (overgo for marker SSR593). Marker not found in sequence. BAC not on Chromosome 4 from FISH analysis performed at Wageningen University (single focus in euchromatin-heterochromatin border region).
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