### Clone hba-115k16

 Clone and library
Clone
Clone name
hba-115k16
LE_HBa0115K16
C10HBa0115K16
P115K16
Clone type
BAC
Contamination
none
Estimated length
88,437
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
 Physical mapping
 Fingerprint Contig Builds (FPC)
Tomato FPC (AGI 2005)
ctg1375 : [coherent], 10 members:
C10HBa0029I02, LE_HBa0042B06, LE_HBa0104H22, C10HBa0115K16, LE_HBa0154L01, C10HBa0238G16, LE_HBa0239H21, LE_HBa0246C08, C10HBa0304I01, LE_HBa0323M08
 Marker matches - overgo what's this?
Conflicted matches
None

 Marker matches - BLAST None
 Marker matches - manual None
 Physical map (from marker matches)
 FISH map
 FISH images
(percentage of arm length)
653-03 1CompositeFluorescencePhase12.7%
653-05 1CompositeFluorescencePhase12.6%
653-06 1CompositeFluorescencePhase12.2%
653-08 1CompositeFluorescencePhase10.1%
653-11 1CompositeFluorescencePhase11.8%
653-12 1CompositeFluorescencePhase11.7%
653-13 1CompositeFluorescencePhase12.3%
653-21 1CompositeFluorescencePhase11.3%
653-23 1CompositeFluorescencePhase12.5%
653-24 1CompositeFluorescencePhase10.0%
653-25 1CompositeFluorescencePhase8.5%
653-26 1CompositeFluorescencePhase11.7%
653-37 1CompositeFluorescencePhase6.8%
653-48 1CompositeFluorescencePhase13.3%
653-49 1CompositeFluorescencePhase7.4%
653-51 1CompositeFluorescencePhase7.8%
Mean±sd10.8%±2.1%

Given an estimated length of 10.1µm for chromosome 10Q and the mean percentage above, this BAC is approximately 1.1µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
 IL Mapping None
 Sequencing
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
End Sequences
Warnings
• poor correspondence with in vitro restriction fragments
(match score below 0.7)
• sequence length 163,868 is 85.3% different from estimated length 88,437
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly

Restriction Fragments
 HindIII in vitro (20) 2068, 2128, 2301, 2301, 2779, 2979, 2979, 3265, 3410, 3644, 3844, 4088, 4088, 5061, 5913, 5913, 6378, 6550, 9374, 9374 HindIII in silico (50) 1002, 1008, 1010, 1014, 1052, 1117, 1117, 1151, 1184, 1250, 1363, 1469, 1480, 1480, 1502, 1714, 1769, 1776, 1834, 2023, 2039, 2106, 2113, 2113, 2241, 2301, 2309, 2320, 2744, 2800, 2954, 2986, 3030, 3287, 3420, 3638, 3704, 3852, 4095, 4151, 4859, 5547, 5567, 5686, 6214, 6257, 6412, 6562, 9279, 9517 Fragment Lengths Match Score 0.63
 Annotations
Browse
LE_HBa0115K16 has no browsable sequence annotations