Clone hba-203j14

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Clone
Clone name
hba-203j14
LE_HBa0203J14
C09HBa0203J14
P203J14
Clone type
BAC
Contamination
none
Estimated length
118,547
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3960 : [coherent], 3 members:
LE_HBa0136D21, C09HBa0203J14, LE_HBa0260J17
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
TG9Tomato-EXPEN 2000plate 14G7
Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
127-07 1CompositeFluorescencePhase75.0%
127-10 1CompositeFluorescencePhase79.9%
127-18 1CompositeFluorescencePhase74.2%
127-22 1CompositeFluorescencePhase76.2%
127-23 1CompositeFluorescencePhase80.6%
127-24 1CompositeFluorescencePhase77.7%
127-25 1CompositeFluorescencePhase76.8%
127-26 1CompositeFluorescencePhase80.5%
Mean±sd77.6%±2.5%

Given an estimated length of 5.4µm for chromosome 9P and the mean percentage above, this BAC is approximately 4.2µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 9 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 9
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
111,868 bp
C09HBa0203J14.1
EF647607.1 (GenBank)
[View]
[Download fasta]
[Download full submission] (502.92 MB)
End Sequences
Warnings
none
FTP
Chromosome Assembly   
Restriction Fragments
HindIII in vitro (26)
1505, 1587, 1679, 1822, 1982, 2013, 2512, 2622, 3221, 3249, 3425, 3453, 3630, 3875, 4328, 4353, 5711, 6127, 6173, 6223, 6223, 6480, 6480, 6999, 6999, 15876
HindIII in silico (27)
1042, 1157, 1501, 1575, 1678, 1812, 1934, 1965, 2006, 2313, 2553, 2648, 2656, 3225, 3439, 3637, 3919, 4279, 4382, 5393, 5888, 6025, 6139, 6435, 7030, 7067, 15303
Fragment Lengths Match Score
0.96
Annotations  
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LE_HBa0203J14 has no browsable sequence annotations
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