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Clone hba-256l16

Clone and library  
Clone
Clone name
hba-256l16
LE_HBa0256L16
C10HBa0256L16
P256L16
Clone type
BAC
Contamination
none
Estimated length
121,657
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg3302 : [coherent], 1 members:
C10HBa0256L16
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
455-01 1CompositeFluorescencePhase77.9%
455-02 1CompositeFluorescencePhase77.9%
455-05 1CompositeFluorescencePhase77.6%
455-06 1CompositeFluorescencePhase80.4%
455-08 1CompositeFluorescencePhase80.4%
455-09 1CompositeFluorescencePhase79.4%
455-10 1CompositeFluorescencePhase79.0%
455-14 1CompositeFluorescencePhase77.4%
455-16 1CompositeFluorescencePhase78.3%
455-17 1CompositeFluorescencePhase80.4%
455-18 1CompositeFluorescencePhase78.7%
455-20a 1CompositeFluorescencePhase78.2%
455-20b 1CompositeFluorescencePhase77.2%
455-21 1CompositeFluorescencePhase79.5%
455-24 1CompositeFluorescencePhase76.4%
455-27 1CompositeFluorescencePhase79.4%
455-28 1CompositeFluorescencePhase80.0%
455-30 1CompositeFluorescencePhase78.3%
455-34 1CompositeFluorescencePhase77.5%
455-36 1CompositeFluorescencePhase76.8%
correction 250.5%
Mean±sd77.2%±1.2%

Given an estimated length of 10.1µm for chromosome 10Q and the mean percentage above, this BAC is approximately 7.8µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

2The position of this BAC has been adjusted slightly to reflect its location relative to the typical position of marker BAC LE_HBa0234C10.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 10 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 10
HTGS phase
3 - finished
Sequencing Status
in_progress
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
159,015 bp
C10HBa0256L16.2
AC233130.7 (GenBank)
[View]
End Sequences
None
Warnings
  • sequence is not finished to HTGS3
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (27)
1988, 2021, 2197, 2311, 2409, 2409, 2702, 2872, 2938, 3044, 3163, 3230, 3380, 3494, 3695, 3814, 3814, 4411, 4803, 5084, 5389, 5389, 6020, 7204, 7935, 11051, 14890
HindIII in silico (45)
1045, 1066, 1190, 1198, 1255, 1270, 1395, 1556, 1592, 1626, 1770, 1810, 1893, 1933, 1968, 1970, 1995, 2191, 2217, 2329, 2391, 2423, 2440, 2702, 2741, 2867, 2965, 3038, 3181, 3248, 3406, 3528, 3768, 3841, 3900, 4421, 4667, 4855, 5082, 5085, 5752, 7216, 7909, 10811, 14374
Fragment Lengths Match Score
0.75
Annotations  
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LE_HBa0256L16 has no browsable sequence annotations
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