Clone Read LE_HBa0144P09_T7_100961

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0144P09_T7_100961
Primer
T7
ID from Seq. Facility
LE_HBa_144_P09-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
711 trimmed for vector and quality, 906 raw
Genbank Accession
DU091686
Sequence
>LE_HBa0144P09_T7_100961 (raw untrimmed)
CTGGGGGCCGGGTCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCGGAAGCAGGATTTGATGTCCTAGGTTTCATCATGTCTGATGGAT
CCCATTTGTCTCCGGCTACTTTTGTTCTTTGCATTGATGTAGCAAGAAATTTTGCAGAGTCTCGTGTTGGACCAGCCGATAGACCTATTCGTGCGGTGGA
TCTTATGACAGGTTCTGCCGCTTGTTTAGCAATCTGGTCTAAAGATACCAGGGAAGCGATGGCAGAGGCAGAAGCTTTGAAGCTGTCTCAGGATATAGGG
GAAATGTGGCTGAGGCTTGTACAGGGTCTGAGGAAAGTTTGTCTGGATCAGAGAGAAGTTAGGAATCATGCTCTTTCATCCCTACAGACGTGCTTGACAG
GAGTAGATGAAATGTACCTTTCACATGGTCTCTGGTTGCAGTGCTTTGATATTGTCATTTTCACAATGCTGGACGACTTGATTGAGCTCACATCACAGAA
AGATTATAGAAACATGGAAGAGACACTTATTCTTGCCTTGAAGCTGTTAACCAAGGTGTTTTTACAATTGCTTCATGAGCTATCTCAATTGACCACCTTC
TGTAAACTCTGGCTTGGAGTCCTCAACCGGATGGAGAAATACATGAAGGTGAAGGTCAGAGGTAAGAAGAGTGAAAAACTTCAGGAGCTAGTCCCTGAAC
TTTCTTAAGAACACCTTGGTTGTAATGAAATCGAAGGGGGTACTCGTTCAGAGGGGTGCTCCTCGTGGAAATAGCTTGTGGGAACTGAACTGTGTGCATG
TCAATAACATTGTTCCTTCTCTGCAACCTGAGGTTTTCCCTAAAAATAGATTGGGGCTTGTAGAGTCTGACCTAACCGACGCCAGATGAGCTGCATTTGA
TGTTGC
>LE_HBa0144P09_T7_100961 (trimmed for quality and cloning vector)
AGCTTCGGAAGCAGGATTTGATGTCCTAGGTTTCATCATGTCTGATGGATCCCATTTGTCTCCGGCTACTTTTGTTCTTTGCATTGATGTAGCAAGAAAT
TTTGCAGAGTCTCGTGTTGGACCAGCCGATAGACCTATTCGTGCGGTGGATCTTATGACAGGTTCTGCCGCTTGTTTAGCAATCTGGTCTAAAGATACCA
GGGAAGCGATGGCAGAGGCAGAAGCTTTGAAGCTGTCTCAGGATATAGGGGAAATGTGGCTGAGGCTTGTACAGGGTCTGAGGAAAGTTTGTCTGGATCA
GAGAGAAGTTAGGAATCATGCTCTTTCATCCCTACAGACGTGCTTGACAGGAGTAGATGAAATGTACCTTTCACATGGTCTCTGGTTGCAGTGCTTTGAT
ATTGTCATTTTCACAATGCTGGACGACTTGATTGAGCTCACATCACAGAAAGATTATAGAAACATGGAAGAGACACTTATTCTTGCCTTGAAGCTGTTAA
CCAAGGTGTTTTTACAATTGCTTCATGAGCTATCTCAATTGACCACCTTCTGTAAACTCTGGCTTGGAGTCCTCAACCGGATGGAGAAATACATGAAGGT
GAAGGTCAGAGGTAAGAAGAGTGAAAAACTTCAGGAGCTAGTCCCTGAACTTTCTTAAGAACACCTTGGTTGTAATGAAATCGAAGGGGGTACTCGTTCA
GAGGGGTGCTC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.972343
Expected error %
0.322326
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g13980.1 Score 279 E Value 3e-78 Identity 66.40% Align. Length 708bp (99.58%) Frame 3
68414.m01647 pattern formation protein (EMB30) (GNOM) identical to SP|Q42510; contains Pfam profile PF01369: Sec7 domain
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g39500.1 Score 225 E Value 2e-60 Identity 50.70% Align. Length 696bp (97.89%) Frame 3
68418.m04783 pattern formation protein, putative similar to SP|Q42510 Pattern formation protein EMB30 {Arabidopsis thaliana}; contains Pfam profile PF01369: Sec7 domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAA91150.1| Score 279 E Value 1e-76 Identity 66.40% Align. Length 708bp (99.58%) Frame 3
GNOM gene product
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD39284.1| Score 279 E Value 1e-76 Identity 66.40% Align. Length 708bp (99.58%) Frame 3
EMB30/GNOM gene product [Arabidopsis thaliana] gb|AAF79403.1| F16A14.20 [Arabidopsis thaliana] ref|NP_172851.1| pattern formation protein (EMB30) (GNOM) [Arabidopsis thaliana] pir||S65571 pattern formation protein GNOM - Arabidopsis thaliana gb|AAB01206.1| similar to the Saccharomyces cerevisiae Sec7 protein, GenBank Accession Number J03918 gb|AAB01205.1| similar to the Saccharomyces cerevisiae Sec7 protein, GenBank Accession Number J03918 gb|AAA91151.1| GNOM gene product sp|Q42510|EM30_ARATH Pattern formation protein EMB30
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0144P09
Clone
Clone name
hba-144p9
LE_HBa0144P09
P144P09
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found