Clone Read LE_HBa0148N20_T7_101655

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0148N20_T7_101655
Primer
T7
ID from Seq. Facility
LE_HBa_148_N20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
727 trimmed for vector and quality, 792 raw
Genbank Accession
DU092161
Sequence
>LE_HBa0148N20_T7_101655 (raw untrimmed)
CTCGGGACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGACACACAAGTATGTATGATTTCCATATACATTCTAGACTCGAACTAGTCA
CTGGTCTGCCAAAACCATATAGTTTGAGAAAGATCATATTTGTGATGCATGCCAAATGGGTAAGCAAACTTGTTCATCCTTCGAAGAAAAAATATTGTAA
GTATATCTAAACCCCTACAACTTCATCATATAGATGTGTTTGGTCCTACTCGAACTACCAACATTAGAGTTAAAAGATATGCTTTTGTAATTGTTGATGA
CTTCTCAAGGTTTACATGGGTAATTTTTCTAACTCGTAAAAATGAAGCTTTTGCTAAGTTTGAAGTATTTTGTAGAAAAGTTCAATTAGAGGCATAATAT
TTCATTACAACTATCCATAGTGATCATGGTGGTGACTTTGAGAATAAGGTCTTTGAAGAATTTTATGCTCAAAACGGTTTACTTAAAATTTCTTTTCACC
CAGATCACCTCAACAAGACGGAGTAGTAGAAAGGAAAAATTGTTCACTACAAGATACAACAAGAACAATGTTTCTAGATCGAAGTCTACCTTATCATTTT
TGTACAGAAGTCGTAAGTATTGCTTGTCATATTCTTAACAGGTGTTAATCAGACCTATACTAAAGAAGACTCCGTATGAATATGGAGAGGTAAGAAACCA
AACATAATCTATTTCCACCCCCTTTGGGTGATAATGTTTCATCCACAATATTCGGTAGGAATAATCTTGGTAAGTTTGATCCAAGAAACAAT
>LE_HBa0148N20_T7_101655 (trimmed for quality and cloning vector)
AGCTTGGACACACAAGTATGTATGATTTCCATATACATTCTAGACTCGAACTAGTCACTGGTCTGCCAAAACCATATAGTTTGAGAAAGATCATATTTGT
GATGCATGCCAAATGGGTAAGCAAACTTGTTCATCCTTCGAAGAAAAAATATTGTAAGTATATCTAAACCCCTACAACTTCATCATATAGATGTGTTTGG
TCCTACTCGAACTACCAACATTAGAGTTAAAAGATATGCTTTTGTAATTGTTGATGACTTCTCAAGGTTTACATGGGTAATTTTTCTAACTCGTAAAAAT
GAAGCTTTTGCTAAGTTTGAAGTATTTTGTAGAAAAGTTCAATTAGAGGCATAATATTTCATTACAACTATCCATAGTGATCATGGTGGTGACTTTGAGA
ATAAGGTCTTTGAAGAATTTTATGCTCAAAACGGTTTACTTAAAATTTCTTTTCACCCAGATCACCTCAACAAGACGGAGTAGTAGAAAGGAAAAATTGT
TCACTACAAGATACAACAAGAACAATGTTTCTAGATCGAAGTCTACCTTATCATTTTTGTACAGAAGTCGTAAGTATTGCTTGTCATATTCTTAACAGGT
GTTAATCAGACCTATACTAAAGAAGACTCCGTATGAATATGGAGAGGTAAGAAACCAAACATAATCTATTTCCACCCCCTTTGGGTGATAATGTTTCATC
CACAATATTCGGTAGGAATAATCTTGG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.952534
Expected error %
0.700097
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAE76041.1| Score 91 E Value 2e-28 Identity 45.90% Align. Length 620bp (85.28%) Frame 3
B1292H11.27 [Oryza sativa (japonica cultivar-group)] ref|XP_471096.1| B1292H11.27 [Oryza sativa (japonica cultivar-group)] emb|CAE03661.3| OSJNBa0042N22.2 [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAD40009.3| Score 89 E Value 3e-29 Identity 44.90% Align. Length 626bp (86.11%) Frame 3
OSJNBb0052B05.12 [Oryza sativa (japonica cultivar-group)] ref|XP_471366.1| OSJNBb0052B05.12 [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0148N20
Clone
Clone name
hba-148n20
LE_HBa0148N20
P148N20
Clone type
BAC
Contamination
none
Estimated length
76,753
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found