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Clone Read LE_HBa0197A16_T7_110231

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0197A16_T7_110231
Primer
T7
ID from Seq. Facility
LE_HBa_197_A16-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
740 trimmed for vector and quality, 957 raw
Genbank Accession
DU095785
Sequence
>LE_HBa0197A16_T7_110231 (raw untrimmed)
TCTACCCGGGTCCCGCGGGTTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTCTGACTTGGTTCGTCTGTACCTATTAAGGCACAAGCCAGCTCCAC
TGTGTCAAAGACTGCTCTTAAGAAGAGCTCAAAAAGGGATGGTGGAGAGGAGAACCCTGAGGATTTTGTTGATCCAGAAACACGTCTGGTTTAGAAGAAG
AAACTCTCCCGAGAAATGGCAAAGACTTTTAATCCCAGTGCTGTAGAGAAATCGTGAGTTCGAAATTGGCTTACCTTAATTTTTCAATTTACTGATTCAG
ACAAAGATTCTATTGTTAGCACACTGTTATGATTCTTTATGCTAACCTGACTAGGTGGTATGCGTGGTGGGAAAAGTCCAATTTCTTTGTTGCAGACCCA
AACAGCGCTAAACCACCTTTTGTGATTGTGAGTTTATCTTCTGACATCTTGTGCTGTTGTTTAAATTGATGCATTTGGTGTGAATGATATTTATGACATA
TATGATCTATAATGAGCTTGGTTCATCTACTAATGCATTTATTGTATTTCTAGTTTTTATGATTATATTTTATGGAGAATCTTTAGTTGGTACTTTTGGT
ATATGATTATTTTTACTTGCTGGGATGTTAATGTTTTGAATTTTTGTCTTTTTTTTTTTTTCTTTTTTATTTTTTTTTTTTTTTTTTTTTTTCTTCCTAC
TATTATCTACTCTCTTTCTCATGGATCACTAATGTACTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT
CTTGTCTGTCCTTTGGTTATACTTCTCTTATCTGATTTCGGAGTGGGGCCTGAACCTCGTCCCCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT
GTTTTCAGCTTTCTACGTTTGGTGTCTACCGCTACATCCACTCTTGTTTTGACTTGT
>LE_HBa0197A16_T7_110231 (trimmed for quality and cloning vector)
AGCTTCTCTGACTTGGTTCGTCTGTACCTATTAAGGCACAAGCCAGCTCCACTGTGTCAAAGACTGCTCTTAAGAAGAGCTCAAAAAGGGATGGTGGAGA
GGAGAACCCTGAGGATTTTGTTGATCCAGAAACACGTCTGGTTTAGAAGAAGAAACTCTCCCGAGAAATGGCAAAGACTTTTAATCCCAGTGCTGTAGAG
AAATCGTGAGTTCGAAATTGGCTTACCTTAATTTTTCAATTTACTGATTCAGACAAAGATTCTATTGTTAGCACACTGTTATGATTCTTTATGCTAACCT
GACTAGGTGGTATGCGTGGTGGGAAAAGTCCAATTTCTTTGTTGCAGACCCAAACAGCGCTAAACCACCTTTTGTGATTGTGAGTTTATCTTCTGACATC
TTGTGCTGTTGTTTAAATTGATGCATTTGGTGTGAATGATATTTATGACATATATGATCTATAATGAGCTTGGTTCATCTACTAATGCATTTATTGTATT
TCTAGTTTTTATGATTATATTTTATGGAGAATCTTTAGTTGGTACTTTTGGTATATGATTATTTTTACTTGCTGGGATGTTAATGTTTTGAATTTTTGTC
TTTTTTTTTTTTTCTTTTTTATTTTTTTTTTTTTTTTTTTTTTTCTTCCTACTATTATCTACTCTCTTTCTCATGGATCACTAATGTACTTTTTTTTTTT
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.909478
Expected error %
3.42596
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g14610.1 Score 70 E Value 4e-20 Identity 55.60% Align. Length 343bp (46.35%) Frame 1
68414.m01737 valyl-tRNA synthetase / valine--tRNA ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS) {Arabidopsis thaliana}
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF63175.1| Score 70 E Value 2e-18 Identity 55.60% Align. Length 343bp (46.35%) Frame 1
T5E21.11 [Arabidopsis thaliana] pir||F86280 protein T5E21.11 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_172913.1| Score 70 E Value 2e-18 Identity 55.60% Align. Length 343bp (46.35%) Frame 1
valyl-tRNA synthetase / valine--tRNA ligase (VALRS) [Arabidopsis thaliana] sp|P93736|SYV_ARATH Valyl-tRNA synthetase (Valine--tRNA ligase) (ValRS)
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0197A16
Clone
Clone name
hba-197a16
LE_HBa0197A16
P197A16
Clone type
BAC
Contamination
none
Estimated length
96,617
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found