Clone Read LE_HBa0118I04_T7_40711

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0118I04_T7_40711
Primer
T7
ID from Seq. Facility
LE_HBa_118_I04-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
797 trimmed for vector and quality, 910 raw
Genbank Accession
DU059957
Sequence
>LE_HBa0118I04_T7_40711 (raw untrimmed)
CCAACCTGGGTACCCGGGGTTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCTTGGAAGTTTGTAAAGGTCCCTATAAGTGCATTTGTATTTTCCTC
TCAATTGAGTCCTCAGCTTTGCGTGGATAAAACTGGGTGAAACCCATGTGCACCTTATAGTTGTGATTAATGTAACTGAGCCAAATTATTGTGACTAGAA
AAGGTTTCAGTAATGATTATGGAATTTCATTCTTCTGAAATTGCTCTGCTAGTTGGTTCTCCTTGCCAACTACACATTTTTCAAACAATTTACACTGGAC
ATTACAAAAAGTTACGGTATTAGCCTTGCTAGAATGAAACAATCAAATCTTTAAGCTGCACCCAACTGTAGGCACATCTATCGCGGAACTTCTCTACAGT
TGATGCTGCTGCTTCAACCACAGGCCGTATCCTAACAAGCTCCTTCTCTACTTGTAATCTCATGTTACTCTCAACCTGCCACTCAAAAAGACAATAGATC
AGCAACTTCCTCACTGGAGGGGGGAATAAAAAACAAAGGGGACCCCAATTTGATGAAATCACACAGATGGGGCCAGAGACAACCTTCTCAATGCAGCGTA
CAGTGTCTAAGACGAAGCAGAAATAAGCAAGCTGTTTGTAAAGATCTGCTTCCGTGTACTGCCAGGGTGAGTTGTGCACAAGTTTTATCAGTAATTAGCT
AAAGCCAACAGTGCAGGTTGACCTAAGAGACGATTCAAAAGATATCAGAAAGTAAACCTGTCTTAGTAAGTGTCCATTACAACGAGGATAATTTGGACAA
ACAGTTCCTCTCTCTGAGTCCCCAATTACTCGCAGGTTCAGGCTACGAGTGGTGTAGTTGCAAGTTTCTTCATCGCACTGAACAGAACAAAATAGCTTTA
GTTAACTGTG
>LE_HBa0118I04_T7_40711 (trimmed for quality and cloning vector)
AGCTTCCTTGGAAGTTTGTAAAGGTCCCTATAAGTGCATTTGTATTTTCCTCTCAATTGAGTCCTCAGCTTTGCGTGGATAAAACTGGGTGAAACCCATG
TGCACCTTATAGTTGTGATTAATGTAACTGAGCCAAATTATTGTGACTAGAAAAGGTTTCAGTAATGATTATGGAATTTCATTCTTCTGAAATTGCTCTG
CTAGTTGGTTCTCCTTGCCAACTACACATTTTTCAAACAATTTACACTGGACATTACAAAAAGTTACGGTATTAGCCTTGCTAGAATGAAACAATCAAAT
CTTTAAGCTGCACCCAACTGTAGGCACATCTATCGCGGAACTTCTCTACAGTTGATGCTGCTGCTTCAACCACAGGCCGTATCCTAACAAGCTCCTTCTC
TACTTGTAATCTCATGTTACTCTCAACCTGCCACTCAAAAAGACAATAGATCAGCAACTTCCTCACTGGAGGGGGGAATAAAAAACAAAGGGGACCCCAA
TTTGATGAAATCACACAGATGGGGCCAGAGACAACCTTCTCAATGCAGCGTACAGTGTCTAAGACGAAGCAGAAATAAGCAAGCTGTTTGTAAAGATCTG
CTTCCGTGTACTGCCAGGGTGAGTTGTGCACAAGTTTTATCAGTAATTAGCTAAAGCCAACAGTGCAGGTTGACCTAAGAGACGATTCAAAAGATATCAG
AAAGTAAACCTGTCTTAGTAAGTGTCCATTACAACGAGGATAATTTGGACAAACAGTTCCTCTCTCTGAGTCCCCAATTACTCGCAGGTTCAGGCTA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.97288
Expected error %
0.398526
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g67100.1 Score 102 E Value 3e-22 Identity 11.80% Align. Length 506bp (63.49%) Frame 3
68418.m08460 DNA-directed DNA polymerase alpha catalytic subunit, putative similar to SP|O48653 DNA polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza sativa}; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD81999.1| Score 114 E Value 3e-24 Identity 12.50% Align. Length 509bp (63.86%) Frame 3
putative DNA polymerase alpha catalytic subunit [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
sp|O48653|DPOA_ORYSA Score 114 E Value 3e-24 Identity 12.50% Align. Length 509bp (63.86%) Frame 3
DNA polymerase alpha catalytic subunit dbj|BAA24573.1| DNA polymerase alpha catalytic subunit [Oryza sativa]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0118I04
Clone
Clone name
hba-118i4
LE_HBa0118I04
C02HBa0118I04
P118I04
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found