Clone Read LE_HBa0009L22_T7_4411

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0009L22_T7_4411
Primer
T7
ID from Seq. Facility
LE_HBa_009_L22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
688 trimmed for vector and quality, 892 raw
Genbank Accession
DU024363
Sequence
>LE_HBa0009L22_T7_4411 (raw untrimmed)
TCTGGGACCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATTTAAGAATTTCTAAGCACATCCCGTTTATTTCTCATAAAGTCACAATTA
TACAACATTTTTATCAATTCACACTCGGGCAATTTTCTCAAAATGCCTAAAAGGCAATTTCTTAATAACACATTAGACAAGTCCACAATTTAACTTCACA
AAGAAAATAGACAATATACACTCAATCCACATGAACATAAGGGAATACAATGTATGCAAACTCATTTTCTCATTTTACAAGAATCTAACCAAAACATGCA
TAACTTAAGGAGAACATTGGAAACTCATTTTTCACAAAGGACTTCATAACATGAATAAGGATACTAAGAACTCTTAGTTTTAGGCTTGAATAAGCATATA
AGGAAAAGATAAGGATATCTCACTCCCGACATCCTTACTATAACAATACTCCCCACATGGGAGAAACACATCTAAGGTCAACATGAAAATATCACCAAAA
ATCTCAATCAAGGAACCATCAACCTTAACAATACCATTCTCACCCTATCCATTCACAATTCCTACTTGTACTCTTTCTCTCCCTCAACCTTTACATACCA
ATTCCTCAAAGGCGACACTCTAAGGGTCATCATATTAGGAATAAGCAAAAGACATCATAAAGCAAAACTCCTAGGCACGATTTGAAGAATGAAGAAGTAA
AACTTTCTTAGAACCCATAGACTCCTATTAATAATGTGGTACACTTCACTTTTTGAACTAGAACCCACTAAATGTGGTTTTTGAAAAAAAATAAGACCCA
TACCAAAAAGCTTTTTGCCCCCGATTTCCAAGTTTTGTATGAACTCAAGAAGCCCCCGCCAAAGATCCCCAAAAATTGGGTGATTTCCAACC
>LE_HBa0009L22_T7_4411 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTATTTAAGAATTTCTAAGCACATCCCGTTTATTTCTCATAAAGTCACAATTATACAACATTTTTATCAATT
CACACTCGGGCAATTTTCTCAAAATGCCTAAAAGGCAATTTCTTAATAACACATTAGACAAGTCCACAATTTAACTTCACAAAGAAAATAGACAATATAC
ACTCAATCCACATGAACATAAGGGAATACAATGTATGCAAACTCATTTTCTCATTTTACAAGAATCTAACCAAAACATGCATAACTTAAGGAGAACATTG
GAAACTCATTTTTCACAAAGGACTTCATAACATGAATAAGGATACTAAGAACTCTTAGTTTTAGGCTTGAATAAGCATATAAGGAAAAGATAAGGATATC
TCACTCCCGACATCCTTACTATAACAATACTCCCCACATGGGAGAAACACATCTAAGGTCAACATGAAAATATCACCAAAAATCTCAATCAAGGAACCAT
CAACCTTAACAATACCATTCTCACCCTATCCATTCACAATTCCTACTTGTACTCTTTCTCTCCCTCAACCTTTACATACCAATTCCTCAAAGGCGACACT
CTAAGGGTCATCATATTAGGAATAAGCAAAAGACATCATAAAGCAAAACTCCTAGGCACGATTTGAAGAATGAAGAAGTAAAACTTTC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.924239
Expected error %
0.375419
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.65%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.65%) Frame 2
pT7T318U cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0009L22
Clone
Clone name
hba-9l22
LE_HBa0009L22
P009L22
Clone type
BAC
Contamination
none
Estimated length
129,931
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found