Clone Read LE_HBa0001J06_T7_444

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001J06_T7_444
Primer
T7
ID from Seq. Facility
Le-HBa001_J06-T7.ab1
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
744 trimmed for vector and quality, 1384 raw
Genbank Accession
DU020936
Sequence
>LE_HBa0001J06_T7_444 (raw untrimmed)
CAAGCCGGGACCGGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGAAAGGTTCTTTAAGAAAAAAGATCTATAACTGTATCAACAAAATATT
TTCTATGAAATACCTTTTCCTATAAATAATTCTCTCCACAAGAATAGTGAAAAAGAAAAAACCATAATATTTATTTCTCTTTGACTTTTAATAATACTGT
TTTATATCTTAAAATAAATGAAATTCCAAAGAAATTTTTGACGATATTAACTAAAACCTAAAATTTGTAGCCAACTATCAACTAAACTATATATATAAAT
TATACACTTTCGTAGAAAAAAATTTATAACCAGATAAGATCCAAGTTTTGTAAAATCGAAAAATAACAATATGAAAAATAAAGCTCAAGACTTTAAAATA
AAATTGTATTTTCTAATTTTAGTTCTCTCTCAATTTCCTAAGATTTAGAACAATTCTTCCCATTATAAAATGGAACACTATCTTACAATATCAAAATTTG
AAATTGCGTAAATTTAACAAAACTCAACAAATGAAATGAATGTGCTTCTTATTCTTCAAAACATTTAAAAGAAATGCTTCTATGTAACTTCATCATCAAC
AAAGAAGAAATAAATCTATTTCAAATTTTATTTTTTCAATTCACATTACACCTTATCGTTTAAATCTCGATATTTAAAAAAATCAAAAATATTTATTTTA
TTAAATCAAAAGATTCCAGCATGTTAGGCACATGCAACCGACCAGATGTGTGATGCTTATTCAAGTTCATGCATACACATCAATCAATTTCTTCTTTTCA
TTTTCATCTTCTTAAATAATTGATAATTTGGGTAAATATAAAAATCAAAGACACTTACAAATTATTTTAATTTTAAAAGAAAAATAATTTAGCCCCATCT
GAATTTCTAATACACTAAAACTTATACGCCTCCTTAAATTATCGAATAAATTATTCCTAATACTGTTATTCACCCCTCGTATAATTTTCATAAAACATAT
CAAGGTTTTCTAAAAAAAAAAAAAAGGGATTGTTCATATATAAGATTAATACTATTTTAAAATAAAAGGCCCCCTTTAAAAATTTGCTTTTAACCCAATT
TTCTGGGTATTCCTACCAAATTAAACCTTACCCCCCATGAACTTTTTTTTTTTAAAAATTTTTATAAATTTTTAAAAAAATTATTTTTAAACCCTCACCC
CAAAAAAGGGAAAATGGCAATTGTGGTTTCAAAAAAAAAAAAACCCCAAAAAATTTTTATTATTAAAACAAAAAAATTTTTACACAAAAAATTTATATTC
TTCTTAAAAAAAAAAAAAAAAAAAAAAAAATTTTTTTTTTTTTTTTTTTATATAAAAAAAAAAAAAAAAAAAAAAAAAACCTTT
>LE_HBa0001J06_T7_444 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTGAAAGGTTCTTTAAGAAAAAAGATCTATAACTGTATCAACAAAATATTTTCTATGAAATACCTTTTCCT
ATAAATAATTCTCTCCACAAGAATAGTGAAAAAGAAAAAACCATAATATTTATTTCTCTTTGACTTTTAATAATACTGTTTTATATCTTAAAATAAATGA
AATTCCAAAGAAATTTTTGACGATATTAACTAAAACCTAAAATTTGTAGCCAACTATCAACTAAACTATATATATAAATTATACACTTTCGTAGAAAAAA
ATTTATAACCAGATAAGATCCAAGTTTTGTAAAATCGAAAAATAACAATATGAAAAATAAAGCTCAAGACTTTAAAATAAAATTGTATTTTCTAATTTTA
GTTCTCTCTCAATTTCCTAAGATTTAGAACAATTCTTCCCATTATAAAATGGAACACTATCTTACAATATCAAAATTTGAAATTGCGTAAATTTAACAAA
ACTCAACAAATGAAATGAATGTGCTTCTTATTCTTCAAAACATTTAAAAGAAATGCTTCTATGTAACTTCATCATCAACAAAGAAGAAATAAATCTATTT
CAAATTTTATTTTTTCAATTCACATTACACCTTATCGTTTAAATCTCGATATTTAAAAAAATCAAAAATATTTATTTTATTAAATCAAAAGATTCCAGCA
TGTTAGGCACATGCAACCGACCAGATGTGTGATGCTTATTCAAG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.885124
Expected error %
0.377452
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65306.2:1-103 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.30%) Frame 2
Cloning vector pGEM-3Zf(+)
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|M34301.1:10-182 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.17%) Frame 2
Cloning vector pPD18.32
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001J06
Clone
Clone name
hba-1j6
LE_HBa0001J06
P001J06
Clone type
BAC
Contamination
none
Estimated length
109,177
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found