Clone Read LE_HBa0074N03_T7_55541

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0074N03_T7_55541
Primer
T7
ID from Seq. Facility
LE_HBa_074_N03-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
676 trimmed for vector and quality, 876 raw
Genbank Accession
DU080165
Sequence
>LE_HBa0074N03_T7_55541 (raw untrimmed)
AAAATGAGTCTGGTCCGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGACTTATGTGTGCATTGAGAAGAAGCTACTGATCATGTCACGACCC
AAAACGAGACGTGAGTGGCACCCACACTTATCCTACTATGTGAGCGAACCAACCAATCTAAACCCCAACATTTCAACTATAATAAACAGAATATAATGCA
GAAGACTTAAAACTCATTAACGAAATCAATAATTAACTTCTAAAACTCAAAACTTATCATTATCCCCAAAATCTGGAAGTCATCATCACAAGAACATCTA
TCCTCAAATTACTAAATCTAAGAGTATCTAAGAAACTAAAATAAGTAAAAAGAGATGGTCCATGTCCGAACTTCAAGACATCAAGACGCGAAGGAAAGAA
TCCAGTCCGAGCTAGGAACAATAGCTCACCCTGAGATCTGATTATGCTGAAGTCTGGCTAGAGTTGCGGACGAGTCGAAGTCGATGGCACGTTTGCTGCA
CTCCACAAATAACAAAGAAAGAAACATACAAGTAGGGGTCAGTACAAAGCACAAGTACTGAGTAGGTATCATCGGCCACCTTAAAATAGAAAGCAATATA
TATCAGATAATAACATAAAATCGACTACCATACTCAACTGGTGACAACAACAAGTACCATAACCATTGACCACAACCCAAGCACATTTATGAGGACTTAG
GCCTCCACACCATACTCATTTGGGAAATAGGTTCTTTAAATCTGAGTATATTAACATAATTCAAGATTCATTCCCTTTACTATCCTGGTGTCGGAACGGG
ACACCTCCAATCCCCTCATCATTCATTTCCTCTTACTATCCTGGGGTCGGAACGTGATACTCCCATCCCCCACCAC
>LE_HBa0074N03_T7_55541 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTGACTTATGTGTGCATTGAGAAGAAGCTACTGATCATGTCACGACCCAAAACGAGACGTGAGTGGCACCCA
CACTTATCCTACTATGTGAGCGAACCAACCAATCTAAACCCCAACATTTCAACTATAATAAACAGAATATAATGCAGAAGACTTAAAACTCATTAACGAA
ATCAATAATTAACTTCTAAAACTCAAAACTTATCATTATCCCCAAAATCTGGAAGTCATCATCACAAGAACATCTATCCTCAAATTACTAAATCTAAGAG
TATCTAAGAAACTAAAATAAGTAAAAAGAGATGGTCCATGTCCGAACTTCAAGACATCAAGACGCGAAGGAAAGAATCCAGTCCGAGCTAGGAACAATAG
CTCACCCTGAGATCTGATTATGCTGAAGTCTGGCTAGAGTTGCGGACGAGTCGAAGTCGATGGCACGTTTGCTGCACTCCACAAATAACAAAGAAAGAAA
CATACAAGTAGGGGTCAGTACAAAGCACAAGTACTGAGTAGGTATCATCGGCCACCTTAAAATAGAAAGCAATATATATCAGATAATAACATAAAATCGA
CTACCATACTCAACTGGTGACAACAACAAGTACCATAACCATTGACCACAACCCAAGCACATTTATGAGGACTTAG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.954148
Expected error %
0.0248016
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65306.2:1-103 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.59%) Frame 2
Cloning vector pGEM-3Zf(+)
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|S52393.1:14-153 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.44%) Frame 2
{polylinker} [pIC20HE, Plasmid Recombinant, 153 nt]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0074N03
Clone
Clone name
hba-74n3
LE_HBa0074N03
C08HBa0074N03
P074N03
Clone type
BAC
Contamination
none
Estimated length
103,934
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found