Clone Read LE_HBa0048G23_T7_63693

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0048G23_T7_63693
Primer
T7
ID from Seq. Facility
LE_HBa_048_G23-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
650 trimmed for vector and quality, 789 raw
Genbank Accession
DU071881
Sequence
>LE_HBa0048G23_T7_63693 (raw untrimmed)
TGGGTACCCGGGGAATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATATACTATATGAAGAAGAAATTTCGGTATTATTTTATTGTGTCTCGATTTAT
ATTATGGAATTCCTGGCCTGCTACATGTTTGCCTGAAAGCTTTAGTTGATTTTTCTGATGTTAATTCAACTTTTTTGGCAGGACTCCAAAGCACAGGTGA
CTGCAATACACTTGATAATAGGAACTCTTCAGAGAATGCACATCTTTGGTGTTGAGAACAGGGACACGTTAACGCACAAGGCTACAGGGGTAATTATCTT
AGCATCTCCTATTTCCTTTTGCTGAAGTTAATCTATTACAAATTAATTATAATCACTGTTGCCCTTTTCACCCTCCAGTACTCTGCAAAGCTCTTGAAGA
AACCTGATCAATGTAGAGCTGTGTATGCTTGTTCGCATCTTTTCTGGGTTGACGATCAGGACAATATCAAGGATGGAGAAAGGTTCATAGCTGTGCCTTG
CGTGTTTAGGGGATAAAATATACATTCAAATATGCTATGTCTCTTTGGAGTTCCTTTTAGTTTTCATGCTTTTCTGTAGCCATCTTCTTATGTGTTACTT
ATTTCCCCTATTGATTCAGGGTTTTGCTTTGCTTAAAGCGGGCCTTAAGAATTGCAAATGCTGCTCAACAAATGTCCAATGCTATCCCCGAGGCACCAGT
GTATCTTGCTTGCTCTTTAAAAAATATCTAAAACAAGTAAAATCCCAATCTTCATTTCCCAGTTCCTTTTTTACCCTTCTCAACTACTG
>LE_HBa0048G23_T7_63693 (trimmed for quality and cloning vector)
AGCTTATATACTATATGAAGAAGAAATTTCGGTATTATTTTATTGTGTCTCGATTTATATTATGGAATTCCTGGCCTGCTACATGTTTGCCTGAAAGCTT
TAGTTGATTTTTCTGATGTTAATTCAACTTTTTTGGCAGGACTCCAAAGCACAGGTGACTGCAATACACTTGATAATAGGAACTCTTCAGAGAATGCACA
TCTTTGGTGTTGAGAACAGGGACACGTTAACGCACAAGGCTACAGGGGTAATTATCTTAGCATCTCCTATTTCCTTTTGCTGAAGTTAATCTATTACAAA
TTAATTATAATCACTGTTGCCCTTTTCACCCTCCAGTACTCTGCAAAGCTCTTGAAGAAACCTGATCAATGTAGAGCTGTGTATGCTTGTTCGCATCTTT
TCTGGGTTGACGATCAGGACAATATCAAGGATGGAGAAAGGTTCATAGCTGTGCCTTGCGTGTTTAGGGGATAAAATATACATTCAAATATGCTATGTCT
CTTTGGAGTTCCTTTTAGTTTTCATGCTTTTCTGTAGCCATCTTCTTATGTGTTACTTATTTCCCCTATTGATTCAGGGTTTTGCTTTGCTTAAAGCGGG
CCTTAAGAATTGCAAATGCTGCTCAACAAATGTCCAATGCTATCCCCGAG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.95946
Expected error %
0.804862
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g75850.1 Score 74 E Value 8e-29 Identity 86.50% Align. Length 307bp (47.23%) Frame 3
68414.m08810 vacuolar protein sorting-associated protein 35 family protein / VPS35 family protein similar to vacuolar protein sorting 35 [Mus musculus] GI:11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g17790.1 Score 69 E Value 7e-27 Identity 51.20% Align. Length 445bp (68.46%) Frame 3
68415.m02062 vacuolar protein sorting-associated protein 35 family protein / VPS35 family protein similar to vacuolar protein sorting 35 [Mus musculus] GI:11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_469973.1| Score 75 E Value 2e-27 Identity 82.50% Align. Length 307bp (47.23%) Frame 3
putative vacuolar protein sorting-associated protein [Oryza sativa (japonica cultivar-group)] gb|AAO72382.1| putative vacuolar protein sorting-associated protein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF26771.2| Score 74 E Value 4e-27 Identity 86.50% Align. Length 307bp (47.23%) Frame 3
T4O12.9 [Arabidopsis thaliana] pir||G96787 protein T4O12.9 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0048G23
Clone
Clone name
hba-48g23
LE_HBa0048G23
C02HBa0048G23
P048G23
Clone type
BAC
Contamination
none
Estimated length
43,482
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found