Clone Read LE_HBa0145N22_T7_71707

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0145N22_T7_71707
Primer
T7
ID from Seq. Facility
LE_HBa_145_N22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
758 trimmed for vector and quality, 843 raw
Genbank Accession
DU063902
Sequence
>LE_HBa0145N22_T7_71707 (raw untrimmed)
TCTAAATCTGGCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTATTCGTATATCACTCTATGTACTCCGATTTCAATAGTACTCAAAG
TCGATATATTAAAACATTAAACGTTATTTCAAAGAAAAATTATTGTTTCAATGTACAAGAATTTTATGTTTTGTCGTGACAAGGATTAATAAAGTATAGT
TTTAGAACTTTTCAGCAAATTGGAAAAGTTCAATAGGGGAAAATATTTTAAACTTTTAAGCAAACTTAATTAATTGTATTGCAGGACACTTTGAAATCTC
TACCCTAGAAGATTAAAATGCAAAATGAATCGAAGAATCTTTGGAGGATATAGAAAGCAACATGAATGTAGTTGTACCTTTATTCCTCAATTAGTTGATC
AAGAATTAAAAGAGACTTGCATCGGTATGAAATTTCAATCACTTGATATTGAATTTAAATTTTATCTTGAGTATGCTTATTGTAATGGTTTTAGTGTGCA
CAAAAATCAAATTAGTAGATTAAGAAAAGATAAATCAATTATTGGTCAAGAGTTGTTTGTTCAAAAAAAAGATTTCGTTCAAAGAAAAGTCTTGACAGTA
ATAAGCAATGAGATGAGACTAGAGAAGGTGCAAAGTAATGATATGTATGTCAAAAAACGAAGAAGAAAAATGGGTTATAACTAGACTTGTCTCAAATCAT
AATGATGAACTTGCTTCTTTCAATAGTCAGAAATTCTTGCGATCAAAAAGAAAAAAAAATCAGAGGCTCTGAATAATCTTATTGATGTCTTAAATAATTT
TGATATTCGTCCAAGTAAAATACATTAGTGTTGATTACTCAGG
>LE_HBa0145N22_T7_71707 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTTTATTCGTATATCACTCTATGTACTCCGATTTCAATAGTACTCAAAGTCGATATATTAAAACATTAAACG
TTATTTCAAAGAAAAATTATTGTTTCAATGTACAAGAATTTTATGTTTTGTCGTGACAAGGATTAATAAAGTATAGTTTTAGAACTTTTCAGCAAATTGG
AAAAGTTCAATAGGGGAAAATATTTTAAACTTTTAAGCAAACTTAATTAATTGTATTGCAGGACACTTTGAAATCTCTACCCTAGAAGATTAAAATGCAA
AATGAATCGAAGAATCTTTGGAGGATATAGAAAGCAACATGAATGTAGTTGTACCTTTATTCCTCAATTAGTTGATCAAGAATTAAAAGAGACTTGCATC
GGTATGAAATTTCAATCACTTGATATTGAATTTAAATTTTATCTTGAGTATGCTTATTGTAATGGTTTTAGTGTGCACAAAAATCAAATTAGTAGATTAA
GAAAAGATAAATCAATTATTGGTCAAGAGTTGTTTGTTCAAAAAAAAGATTTCGTTCAAAGAAAAGTCTTGACAGTAATAAGCAATGAGATGAGACTAGA
GAAGGTGCAAAGTAATGATATGTATGTCAAAAAACGAAGAAGAAAAATGGGTTATAACTAGACTTGTCTCAAATCATAATGATGAACTTGCTTCTTTCAA
TAGTCAGAAATTCTTGCGATCAAAAAGAAAAAAAAATCAGAGGCTCTGAATAATCTTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.918449
Expected error %
0.269686
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02430.1:1751-1832 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.09%) Frame 2
Cloning vector pMAMneoBlue
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U02437.1:4662-4674-49 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.09%) Frame 2
Cloning vector pT3/T7-LUC
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||S50324 Score 93 E Value 7e-18 Identity 21.60% Align. Length 212bp (27.97%) Frame 3
hypothetical protein - tomato gb|AAA62272.1| ORF
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0145N22
Clone
Clone name
hba-145n22
LE_HBa0145N22
P145N22
Clone type
BAC
Contamination
none
Estimated length
104,355
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found