Clone Read LE_HBa0146E01_T7_72001

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0146E01_T7_72001
Primer
T7
ID from Seq. Facility
LE_HBa_146_E01-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
580 trimmed for vector and quality, 853 raw
Sequence
>LE_HBa0146E01_T7_72001 (raw untrimmed)
GGTTAGGCGGGGCCCGGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTTGATCATATGATGTAATCATTCGACAAAATTTTTTTAAATTCA
ATGTATTATTCGATCTCTTTCAAAGTGTAACGTATTATATGAATCTTAAATAAAGTTAGTTGAAATATTCTTATAAATATTTGTTATTGTTCTCTTCATT
TGCTTTCGTTTAATTATTTGGGAACTAGTTCTCTTCTCTTTTCTTTTCCCCCTTTTTCTTTGTGGGAAATTGATTATTCTCCTACTTTAATTCAATTAAG
ATAAAAAGAAAACTATTCGTTTTTAAAAGCTTTTTTTTATGTTTTAATTTTATTATTTTATATTTTCTCAAATAACGAAGTTGTATCTCTTCTTGAGTTC
TTAAATTTTGTTCTAAATTTAATTTAATTATGTGATTAAAGAATCTCTTTTTAATTGCTCTGAAACAATTGAGCGATTTTCTAGAAGAAATATAGGATTC
TGCTTCTGGAGGACACATGATTGATCCTGCTTATGGGGTCAAGCATAACTCTTTTTATGGTCTTTCTGCGTTATTATGGTCTTTATGCGCATGTAATATT
CAATGATATTTAAATGGAAAAGGGTCTGATATACCCCTCAACGTTGTCGTTTGGAAGCTGATATACCTCTTGTTATGAAAGTGGGCTCATATATACCCCT
ACTTGTGACAAATGACCCTCGTATACCCTCTTTTTTCCTAACAGAAAGGAAAATAAAATTTTTGTCTGAATTTTTTATTTTTTTCCTTAAAATGTAACCT
TTTTGAGTCAATTTATGTCCCCCCCTTTACCGATTTGTGGCCTTTTCTTGATT
>LE_HBa0146E01_T7_72001 (trimmed for quality and cloning vector)
AGCTTTTTTGATCATATGATGTAATCATTCGACAAAATTTTTTTAAATTCAATGTATTATTCGATCTCTTTCAAAGTGTAACGTATTATATGAATCTTAA
ATAAAGTTAGTTGAAATATTCTTATAAATATTTGTTATTGTTCTCTTCATTTGCTTTCGTTTAATTATTTGGGAACTAGTTCTCTTCTCTTTTCTTTTCC
CCCTTTTTCTTTGTGGGAAATTGATTATTCTCCTACTTTAATTCAATTAAGATAAAAAGAAAACTATTCGTTTTTAAAAGCTTTTTTTTATGTTTTAATT
TTATTATTTTATATTTTCTCAAATAACGAAGTTGTATCTCTTCTTGAGTTCTTAAATTTTGTTCTAAATTTAATTTAATTATGTGATTAAAGAATCTCTT
TTTAATTGCTCTGAAACAATTGAGCGATTTTCTAGAAGAAATATAGGATTCTGCTTCTGGAGGACACATGATTGATCCTGCTTATGGGGTCAAGCATAAC
TCTTTTTATGGTCTTTCTGCGTTATTATGGTCTTTATGCGCATGTAATATTCAATGATATTTAAATGGAAAAGGGTCTGA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.886699
Expected error %
0.918682
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 86 E Value 1e-18 Identity 85.00% Align. Length 106bp (18.28%) Frame 1
Nicotiana tabacum plastid, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0146E01
Clone
Clone name
hba-146e1
LE_HBa0146E01
P146E01
Clone type
BAC
Contamination
none
Estimated length
86,418
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found