SGN Marker SSR526

SGN-M1199

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 45 High: 50
Repeat motif: AT   Repeat number: 11
Repeat motif: AAAAC   Repeat number: 2
Repeat motif:    Repeat number:
Forward primer: AGGGTCCTTCGTTTGGAACT
Reverse primer: GCATTCCACTTGTGAAGCAT
Predicted size: 230
Mapped locations  
Map: Tomato-LXCHM 2007   
Map version
68 (location: 42997)
Chromosome
10
Position
23.00 cM
Confidence
uncalculated
Protocol
SSR


Tomato-LXCHM 2007

PCR data   Exp. ID 43997
Forward primer (5'-3')
AGGGTCCTTCGTTTGGAACT
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
GCATTCCACTTGTGAAGCAT
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39259)
Chromosome
10
Position
33.70 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 127
Forward primer (5'-3')
AGGGTCCTTCGTTTGGAACT
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
GCATTCCACTTGTGAAGCAT
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 230+320
Solanum lycopersicum LA925: 230+230
Approximate temperature
60°C
  
Overgo hybridization and physical mapping 
SSR526 was used as an overgo probe on plate 6 [well B1]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
AATCCCGTATTCTTCCGTAGGATG
B sequence
CGCGGGTGGATCTTTTCATCCTAC
AB sequence
AATCCCGTATTCTTCCGTAGGATGAAAAGA
Marker sequence
AAAATCCCGTATTCTTCCGTAGGATGAAAAGATCCACCCGCGGCCGCTCT
AGAAGTACTCTCGAGTTTTTTTTTTTTTTTTTTTATTGGGCAAAAAATGA
ATCAAACATTACTGGGTTGGGTGTTTACACAATAACTCAACCATTGATTG
TAAGAAAAAAATCACTTGTGTGCTGCAAGCACAAGCAATACAAATTGATT
GAAAGCTCTTACTGTGGGATGTATTCATTTTTTTCTTAGGACACGATACT
CAAACCTAGATTCACTCAAATCTAGATTCTTGTAATTATGATTCAAGTGA
AGAAAAACTAAGCCTATTTATTTCAGGCCCCTCGGGACTTGACGCTTGGA
CACCGGGAAGCTTGTCCCAGGGTCCTTCGTTTGGAACTGCCTTTTTCTTT
TCCAAAGTGGAATTCCTCCTTTCTTTCCCCTTTGACCTGTGCCAAAAAAC
AAAACAATATATATATATATATATATATATATATATACATATATATATAT
ACCGAATGCCTCCAACAAAATTGCTGAATCAACCATGTAGGTTCACATTA
AACAATCTTTTTTTTTAATTCAGGTCTGATGCTTCACAAGTGGAATGCCN
GATTGTCGTGGCAATTGGCATACGATAATTTACACAGCTGCAGATGCTTA
ATACCTGTTGGAAAAAGATAGTATTCTTCAATGAGTGGNTGAGGTGAAAA
TTGCAAGCTGCTGATAGACTTTGCTTGCGTGGTCAAA
Genomic location of SSR526  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D1199 marker 1199
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