SGN Marker TG554

SGN-M196

SynonymsNone
Locus associationsNone
RFLP details 
Insert size
950
Vector
pGEM4Z
Cutting Site
PST1
Drug Resistance
AMP
Image
Forward sequence
AAAAAATCTGTTTCTCGGGAATTGAAGAGTCAGTGATAGAACCCTGCTGTTGCTGATACTCAGAAAATAGTACAAATACTTTGCTCTGGG
GGGTTCCTTTAACCCAGCCATCTCTGAAATGACCCTTTGTATGGCCTGCTATGGCCTGGCTTGAAGGGAAGTTTTTTTTACAATGGATGC
ATTGGTACATCCACATACCCTGCTGCCCATTTTGTCCCTGTATTGTTGTTACTTCCATGTTAATCGAAGAAGAGGAATACATATAAAGGA
GCTCTAACCAAGTAATTTTTAAAAACAGAGTTGGCATAAAAAAAGTCATTGCATCTTAAAGATATAACAACAAGGAGTACACAATTGCAA
CTCCGTTATTTTGGTTTTATCTTTTTCTATTTGACCATAAATAATGGAACTCTTAAGTTTCTTTTTTTCAATTGATTTTGTCCTAATAAA
TAAATAGTTGAAATATTTGCTTTTAA
Reverse sequence
ATATTTACTACAGTTGTCTTCTTCTCTTCATTTAATGAGATATCAGTGATGCCTCATCAGAACCAGGTATTATTGTCATCAGGGAATAGA
GTGAATTGTGAAACAAATAGGAAATAATGCAATACTTGGGTAGAGTTTCAGTGAATATAATATCCCTTCTCTTATAAGGTAATTAGGGAA
TGAAAATCCTACATATGACAAGTAAGAAGAATCATTGGAAGGAATGAACAATATATGTCACATCCTAATTCTATTCTGTGATAAGGTTCC
CTGGTTCATAAACTCTAAGCTGACTGGTCATATCTCTCTACCAAGAGAAAATAGGGAAAATACCATACTCTTAAGATTCTATAAACCCTT
GATTGGTACATATACTTTTTAAGTTTGTAACTCTACCTGCTTTCTTAACCTAGTGATGGAGTTACCTAATGTGTTGAACCCCTGAAGGAT
CAAGGACCATAAATAGTTGGAACAAGTCATCATTGCCTTTTA
RFLP sequence matches to unigenes 
Unigenee valueMatch length (bp)Strand
SGN-U1521528e-108220 (46.2% of RFLP seq)+
SGN-U1540501e-125268 (56.3% of RFLP seq)+
SGN-U1521527.6e-3167 (13.6% of RFLP seq)-
SGN-U154050324 (65.9% of RFLP seq)-
Mapped locations  
Map: Tomato QTL map   
Map version
137 (location: 53902)
Chromosome
2
Position
22.30 cM
Confidence
uncalculated
Protocol
RFLP

Dry_matter_weight_dmw2_1 Titratable_acidity_ta2_1 Eugenol_content_eug2_1 Fruit_weight_fw2_1Soluble_solid_content_ssc2_1 Sugar_content_suc2_1 3_Methylthio_propanal_content_mta2_1 Hex_3_en_1_ol_content__x3o2_1 Fruit_diameter_fd2_1 Color_a_a2_1 Dry_matter_weight_dmw2_2 Sugar_content_suc2_2 Soluble_solid_content_ssc2_2 Color_L_L2_1 Fruit_weight_fw2_2Titratable_acidity_ta2_2 Dry_matter_weight_dmw2_3 Color_b_b2_1Fruit_diameter_fd2_2 Elasticity_ela2_1 Carotene_content_car2_1 2_Isobutylthiazole_content_ibt2_1
Tomato QTL map

Map: Tomato-EXPIMP 2009   
Map version
126 (location: 53566)
Chromosome
2
Position
31.13 cM
Confidence
uncalculated
Protocol
RFLP


Tomato-EXPIMP 2009

Map: Eggplant-COSII   
Map version
63 (location: 42486)
Chromosome
2
Position
39.00 cM
Confidence
F(LOD3)
Protocol
RFLP


Eggplant-COSII

Map: Tomato-EXPEN 2000   
Map version
52 (location: 40084)
Chromosome
2
Position
35.00 cM
Confidence
I(LOD2)
Protocol
RFLP


Tomato-EXPEN 2000

Map: Tomato-EXHIR 1997   
Map version
32 (location: 8540)
Chromosome
2
Position
28.50 cM
Confidence
I(LOD2)
Protocol
RFLP


Tomato-EXHIR 1997

Map: Eggplant-LXM 2002   
Map version
29 (location: 8542)
Chromosome
2
Position
33.00 cM
Confidence
F(LOD3)
Protocol
RFLP


Eggplant-LXM 2002

Map: Tomato-EXPEN 1992   
Map version
28 (location: 8541)
Chromosome
2
Position
38.00 cM
Confidence
I(LOD2)
Protocol
RFLP


Tomato-EXPEN 1992
Overgo hybridization and physical mapping 
TG554 was used as an overgo probe on plate 15 [well F11]

Plausible BAC Matches:   P282O02,   P146P12

 Overgo Sequences

A sequence
CCAGCCATCTCTGAAATGACCCTT
B sequence
CATAGCAGGCCATACAAAGGGTCA
AB sequence
CCAGCCATCTCTGAAATGACCCTTTGTATG
Marker sequence
AAAAAATCTGTTTCTCGGGAATTGAAGAGTCAGTGATAGAACCCTGCTGT
TGCTGATACTCAGAAAATAGTACAAATACTTTGCTCTGGGGGGTTCCTTT
AACCCAGCCATCTCTGAAATGACCCTTTGTATGGCCTGCTATGGCCTGGC
TTGAAGGGAAGTTTTTTTTACAATGGATGCATTGGTACATCCACATACCC
TGCTGCCCATTTTGTCCCTGTATTGTTGTTACTTCCATGTTAATCGAAGA
AGAGGAATACATATAAAGGAGCTCTAACCAAGTAATTTTTAAAAACAGAG
TTGGCATAAAAAAAGTCATTGCATCTTAAAGATATAACAACAAGGAGTAC
ACAATTGCAACTCCGTTATTTTGGTTTTATCTTTTTCTATTTGACCATAA
ATAATGGAACTCTTAAGTTTCTTTTTTTCAATTGATTTTGTCCTAATAAA
TAAATAGTTGAAATATTTGCTTTTAA
Genomic location of TG554  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D196 marker 196
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