Sol Genomics Network

SGN Marker C2_At2g25950

SGN-M4714
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303864 (SGN) 123150 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201087	-	-	-
Lycopersicon Combined	Single	SGN-U221057	-	-	-
Solanum tuberosum	Single	SGN-U264694	-	-	-
Arabidopsis	Single	At2g25950 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38328)
Chromosome 8
Position 23.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39586

Forward primer (5'-3')

TGGTGGTGCTGATGGAACAAGTCC

Accessions and product sizes

Solanum pennellii TA56

350

Solanum lycopersicum TA209

350

Approximate temperature

55°C

Reverse primer (5'-3')

TCGCTTGCATAGATTGAGCATCTG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

110+240

Solanum lycopersicum TA209

350

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25717)
Chromosome 2
Position 11.08 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10665
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 10666
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 10667
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 12988
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 350	Approximate temperature 55°C

PCR data Exp. ID 12989
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 400	Approximate temperature 55°C

PCR data Exp. ID 12990
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 350	Approximate temperature 55°C

PCR data Exp. ID 12991
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 12992
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 300	Approximate temperature 55°C

PCR data Exp. ID 12993
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C

PCR data Exp. ID 12994
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 300	Approximate temperature 55°C

PCR data Exp. ID 12995
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Physalis TA1367: 350	Approximate temperature 55°C

PCR data Exp. ID 12996
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 350	Approximate temperature 55°C

PCR data Exp. ID 12997
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 350	Approximate temperature 55°C

PCR data Exp. ID 12998
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 350	Approximate temperature 55°C

PCR data Exp. ID 32926
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 32927
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 300	Approximate temperature 55°C

PCR data Exp. ID 32928
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C

PCR data Exp. ID 10668
Forward primer (5'-3') TGGTGGTGCTGATGGAACAAGTCC	Digested band sizes (using AluI) Solanum lycopersicum LA925: 350 Solanum pennellii LA716: 240+110	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCGCTTGCATAGATTGAGCATCTG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, HinfI
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 55°C