Sol Genomics Network

SGN Marker C2_At2g22570

SGN-M4717
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	307072 (SGN) 128866 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200076	-	-	-
Solanum tuberosum	Single	SGN-U248441	-	-	-
Arabidopsis	Single	At2g22570 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38317)
Chromosome 11
Position 31.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39578

Forward primer (5'-3')

ACTGAAGAGTGAGATTCCGGTGGAG

Accessions and product sizes

Solanum pennellii TA56

1200

Solanum lycopersicum TA209

1200

Approximate temperature

55°C

Reverse primer (5'-3')

TCTGTTCCAGTGATACAATGAGGAGG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

750

Solanum lycopersicum TA209

1200

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25691)
Chromosome 2
Position 9.60 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10634
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1200	Approximate temperature 55°C

PCR data Exp. ID 10635
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1200	Approximate temperature 55°C

PCR data Exp. ID 10636
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1200	Approximate temperature 55°C

PCR data Exp. ID 12962
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1500	Approximate temperature 55°C

PCR data Exp. ID 12963
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1300	Approximate temperature 55°C

PCR data Exp. ID 12964
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1200	Approximate temperature 55°C

PCR data Exp. ID 12965
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 12966
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 12967
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 12968
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850+1200	Approximate temperature 55°C

PCR data Exp. ID 12969
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1200	Approximate temperature 55°C

PCR data Exp. ID 12970
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Physalis TA1367: 1200	Approximate temperature 55°C

PCR data Exp. ID 12971
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1200	Approximate temperature 55°C

PCR data Exp. ID 12972
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Coffea canephora BP409: 150+350	Approximate temperature 55°C

PCR data Exp. ID 32915
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 32916
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1200	Approximate temperature 55°C

PCR data Exp. ID 32917
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 32918
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850+1200	Approximate temperature 55°C

PCR data Exp. ID 10637
Forward primer (5'-3') ACTGAAGAGTGAGATTCCGGTGGAG	Digested band sizes (using DraI) Solanum lycopersicum LA925: 1200 Solanum pennellii LA716: 750	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTCCAGTGATACAATGAGGAGG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, DraI, EcoRV, DpnII All possible enzymes for lycopersicum and pimpinellifolium: DpnII
Accessions and product sizes Solanum lycopersicum LA925: 1200 Solanum pennellii LA716: 1200	Approximate temperature 55°C