SGN Marker CT232

SGN-M567

SynonymsNone
Locus associationsNone
RFLP details 
Insert size
500
Vector
pBLUESC
Cutting Site
EcoR1
Drug Resistance
AMP
Image
Forward sequence
CTTTTTTTTTTTTTTTGTAGTTGTCCACTGTGGTTGGTGATGTTGTTTTGGCAGCTTGAAGCTATGGGGTTTGATCGAGCTTTAGTATTG
GAAGTATACTTCGCCTGCAACAAAAATGAGGAGCTGGCTGCTAACTATCTGTTAGATCACTTGCATGAGTTTGACGAGTGACATGGATAT
AACATAGTGCTATTGACCTGTTATAGTTTCCATTCTTAGTTGTTTTACTGCTCTTCATTTTAAATCTGCACGACTTGCTATTTATCTTGC
AATGTGGAGTACATTTTATTTTACTCATTCGCAGCAGTAAGCCTTGGATACTCGGCCCCTTTTGTATAATGGATCATATTATATCAGTCA
AACCGTCCTGCAAGCTCTTTTGTCCTTGGACCCTTTTGTAAAAAANAGAAAAAAAAAAAAAA
Reverse sequence
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAAAAGGGGCC
CAGGAAAAAAAAACTTGCGGGGAGGGTTGACCGAAAAAAAATGGCCCCTTTAACAAAAGGGGGCCAAAATCCCAGGGTTTCCGGGGGGAA
AGAAAAAAAAAAAAA
RFLP sequence matches to unigenes 
Unigenee valueMatch length (bp)Strand
SGN-U1486623e-0536 (8.5% of RFLP seq)+
SGN-U1486743e-0544 (10.4% of RFLP seq)+
SGN-U1435792.1e-1569 (16.4% of RFLP seq)+
SGN-U1486751.6e-31124 (29.4% of RFLP seq)+
SGN-U148662319 (75.6% of RFLP seq)+
Mapped locations  
Map: Eggplant-RAD 2012   
Map version
174 (location: 132210)
Chromosome
2
Position
85.12 cM
Confidence
uncalculated
Protocol
CAPS


Eggplant-RAD 2012

Map: Eggplant-COSII   
Map version
63 (location: 42333)
Chromosome
2
Position
75.70 cM
Confidence
F(LOD3)
Protocol
RFLP


Eggplant-COSII

Map: Pepper-COSII   
Map version
58 (location: 41575)
Chromosome
2
Position
43.40 cM
Confidence
I(LOD2)
Protocol
dCAPS


Pepper-COSII

PCR data   Exp. ID 43546
Forward primer (5'-3')
TGTCTACCCTGCTGCACAAC
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
GAGTATCAGGTACTTTTGTGGAATGT
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39027)
Chromosome
2
Position
90.10 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

Map: Eggplant-LXM 2002   
Map version
29 (location: 9498)
Chromosome
2
Position
71.00 cM
Confidence
F(LOD3)
Protocol
RFLP


Eggplant-LXM 2002

Map: Tomato-EXPEN 1992   
Map version
28 (location: 9497)
Chromosome
2
Position
76.10 cM
Confidence
I(LOD2)
Protocol
RFLP


Tomato-EXPEN 1992
Overgo hybridization and physical mapping 
CT232 was used as an overgo probe on plate 16 [well F9]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
ATCAGTCAAACCGTCCTGCAAGCT
B sequence
GGTCCAAGGACAAAAGAGCTTGCA
AB sequence
ATCAGTCAAACCGTCCTGCAAGCTCTTTTG
Marker sequence
CTTTTTTTTTTTTTTTGTAGTTGTCCACTGTGGTTGGTGATGTTGTTTTG
GCAGCTTGAAGCTATGGGGTTTGATCGAGCTTTAGTATTGGAAGTATACT
TCGCCTGCAACAAAAATGAGGAGCTGGCTGCTAACTATCTGTTAGATCAC
TTGCATGAGTTTGACGAGTGACATGGATATAACATAGTGCTATTGACCTG
TTATAGTTTCCATTCTTAGTTGTTTTACTGCTCTTCATTTTAAATCTGCA
CGACTTGCTATTTATCTTGCAATGTGGAGTACATTTTATTTTACTCATTC
GCAGCAGTAAGCCTTGGATACTCGGCCCCTTTTGTATAATGGATCATATT
ATATCAGTCAAACCGTCCTGCAAGCTCTTTTGTCCTTGGACCCTTTTGTA
AAAAANAGAAAAAAAAAAAAAA
Genomic location of CT232  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D567 marker 567
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