SGN Marker C2_At1g10030

SGN-M6570
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
Capsicum annuumSingleSGN-U197900---
Lycopersicon CombinedSingleSGN-U223854---
Solanum tuberosumSingleSGN-U264912---
ArabidopsisSingleAt1g10030 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38084)
Chromosome
4
Position
88.70 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39379
Forward primer (5'-3')
AGCTGTTAGGATGGTGGTTAATGC
Accessions and product sizes
Solanum lycopersicum TA209
1650
Solanum pennellii TA56
1650
Approximate temperature
53°C
Reverse primer (5'-3')
ACTCTGTCAAGAAATGACCGAAGGC
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
500+1150
Solanum pennellii TA56
400+1150
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 24968)
Chromosome
1
Position
3.27 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 9984
Forward primer (5'-3')
AGCTGTTAGGATGGTGGTTAATGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTCTGTCAAGAAATGACCGAAGGC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 1500
Approximate temperature
55°C
  
PCR data   Exp. ID 9985
Forward primer (5'-3')
AGCTGTTAGGATGGTGGTTAATGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTCTGTCAAGAAATGACCGAAGGC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 1500
Approximate temperature
55°C
  
PCR data   Exp. ID 9986
Forward primer (5'-3')
AGCTGTTAGGATGGTGGTTAATGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTCTGTCAAGAAATGACCGAAGGC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 1500
Approximate temperature
55°C
  
PCR data   Exp. ID 9987
Forward primer (5'-3')
AGCTGTTAGGATGGTGGTTAATGC
Digested band sizes (using DpnII)
Solanum pennellii LA716: 1100
Solanum lycopersicum LA925: 1500
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
ACTCTGTCAAGAAATGACCGAAGGC
Enzymes
All possible enzymes for pennellii and and lycopersicum: AdeI/DraIII,AluI,ApaLI,BanII,BsiHKAI,BslI,BsoBI,BstUI,BstYI,BtgI,DpnII,HinfI,PflFI,PflMI,RsaI,SsPI,SwaI,TspRI
All possible enzymes for lycopersicum and pimpinellifolium: ApoI,BstZ17I,DraI,EcoRV,SwaI
  
Accessions and product sizes
Solanum pennellii LA716: 1500
Solanum lycopersicum LA925: 1500
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At1g10030F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text
Forward amplicon sequence for S. hirtum 120062 plant #28, AB1 chromatogram - [View]
Forward amplicon sequence for S. hirtum 120062 plant #28, plain text
Forward amplicon sequence for S. quitoense plant #3, AB1 chromatogram - [View]
Forward amplicon sequence for S. quitoense plant #3, plain text
Forward amplicon sequence for S. betaceum 6002061 plant #4, AB1 chromatogram - [View]
Forward amplicon sequence for S. betaceum 6002061 plant #4, plain text
Forward amplicon sequence for S. uniloba 6002078 plant #6, AB1 chromatogram - [View]
Forward amplicon sequence for S. uniloba 6002078 plant #6, plain text

Genomic location of C2_At1g10030  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D6570 marker 6570
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