Sol Genomics Network

SGN-M6692
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301070 (SGN) 121897 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U204056	-	-	-
Lycopersicon Combined	Multiple	SGN-U213459	-	-	-
Solanum tuberosum	Single	SGN-U246571	-	-	-
Arabidopsis	Single	At1g23740 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41056

Forward primer (5'-3')

TGAAGTTGGAGTCCAATGTTGCAG

Accessions and product sizes

Solanum lycopersicum TA209

1350

Solanum pennellii TA56

1350

Approximate temperature

55°C

Reverse primer (5'-3')

ACCGCCAGCACCTCCCAAAAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10087
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 10088
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 10089
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 12514
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 650	Approximate temperature 55°C

PCR data Exp. ID 12515
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1350	Approximate temperature 55°C

PCR data Exp. ID 12516
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12517
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 12518
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 950	Approximate temperature 55°C

PCR data Exp. ID 12519
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 850	Approximate temperature 55°C

PCR data Exp. ID 12520
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12521
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1100	Approximate temperature 55°C

PCR data Exp. ID 12522
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1100	Approximate temperature 55°C

PCR data Exp. ID 12523
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 1100	Approximate temperature 55°C

PCR data Exp. ID 32596
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 32729
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 32730
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 32731
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 32732
Forward primer (5'-3') TGAAGTTGGAGTCCAATGTTGCAG		Mg⁺² concentration 3mM
Reverse primer (5'-3') ACCGCCAGCACCTCCCAAAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 950	Approximate temperature 55°C